Publications by authors named "Hualin Wang"

197 Publications

Non-structural Proteins of Severe Fever With Thrombocytopenia Syndrome Virus Suppress RNA Synthesis in a Transcriptionally Active cDNA-Derived Viral RNA Synthesis System.

Front Microbiol 2021 16;12:709517. Epub 2021 Aug 16.

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by the tick-borne SFTS bunyavirus (SFTSV) resulting in a high fatality rate up to 30%. SFTSV is a negative-strand RNA virus containing three single-stranded RNA genome segments designated as L, M, and S, which respectively, encode the RNA-dependent RNA polymerase (RdRp), glycoproteins Gn and Gc, and nucleoprotein (N) and non-structural proteins (NSs). NSs can form inclusion bodies (IBs) in infected and transfected cells. A previous study has provided a clue that SFTSV NSs may be involved in virus-like or viral RNA synthesis; however, the details remain unclear. Our work described here reveals that SFTSV NSs can downregulate virus-like RNA synthesis in a dose-dependent manner within a cDNA-derived viral RNA synthesis system, i.e., minigenome (-) and minigenome (+) systems based on transfection, superinfection, and luciferase reporter activity determination; meanwhile, NSs also show a weak inhibitory effect on virus replication. By using co-immunoprecipitation (Co-IP) and RT-PCR combined with site-directed mutagenesis, we found that NSs suppress virus-like RNA or virus replication through interacting with N but not with RdRp, and the negative regulatory effect correlates closely with the IB structure it formed but is not associated with its role of antagonizing host innate immune responses. When the cytoplasmic structure of IB formed by SFTSV NSs was deprived, the inhibitory effect of NSs on virus-like RNA synthesis would weaken and even disappear. Similarly, we also evaluated other bandavirus NSs that cannot form IB in neither infected nor transfected cells, and the results showed that the NSs of Heartland bandavirus (HRTV) did not show a significant inhibitory effect on virus-like RNA synthesis within a minigenome system. Our findings provide experimental evidence that SFTSV NSs participate in regulating virus-like or viral RNA synthesis and the negative effect may be due to the NSs-N interaction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.709517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415556PMC
August 2021

pH-responsive antibacterial film based polyvinyl alcohol/poly (acrylic acid) incorporated with aminoethyl-phloretin and application to pork preservation.

Food Res Int 2021 09 17;147:110532. Epub 2021 Jun 17.

School of Food and Biological Engineering, Hefei University of Technology, 230009 Hefei, Anhui, PR China; Anhui Institute of Agro-Products Intensive Processing Technology, 230009 Hefei, Anhui, PR China.

This study demonstrates a pH-responsive antibacterial film based on polyvinyl alcohol/poly (acrylic acid) incorporated with aminoethyl-phloretin (PVA/PAA-AEP) for intelligent food packaging. The thermal, mechanical, barrier and light transmittance properties of PVA/PAA are enhanced by PAA presence of ≤6%. The interactions between PVA and PAA were hydrogen and ester bonds. The pH-responsive characteristic is dependent on the protonation/deprotonation tendency of the carboxylic groups on PAA in acidic/alkaline environment. The PVA/PAA3 is selected for the incorporation of AEP and its pH-responsive swelling follows Ritger-Peppas and Schott second-order models. The AEP is hydrogen bonded with the matrix of PVA/PAA3 and the release of AEP is pH-responsive and a rate-limiting step following the First-order model. With pH decrease, the predominant release control was gradually changing from polymer relaxation to Fick diffusion. The PVA/PAA3-AEP films demonstrate AEP content dependent antioxidant and antimicrobial activities. Furthermore, the antibacterial efficiency against Listeria monocytogenes and Staphylococcus aureus is significantly better than Escherichia coli. The target film PVA/PAA3-AEP3 can effectively prolong the shelf-life of pork (TVB-N < 25 mg/100 g) by 4 days at 25 °C, suggesting its great potential in intelligent food packaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodres.2021.110532DOI Listing
September 2021

Simultaneously achieving high-effective oil-water separation and filter media regeneration by facile and highly hydrophobic sand coating.

Sci Total Environ 2021 Aug 5;800:149488. Epub 2021 Aug 5.

National Engineering Laboratory for Industrial Wastewater Treatment, East China University of Science and Technology, Shanghai 200237, China.

Efficient oil-water separation, including of emulsified oil separation, is one of the problems restricting the green development of the petrochemical industry. Herein, highly hydrophobic sand was fabricated in one-step, followed by an investigation of adsorption capacity for various oils of hexane, petroleum ether, diesel, tetrachloroethylene and tetrachloromethane. The modified sand (MS) filter bed was subsequently set up to investigate the oil separation efficiency for oil-water mixtures, emulsions and actual petroleum refinery wastewater, respectively. Moreover, the capture process of the oil droplet by the MS was observed by a high-speed camera system, and the oil removal mechanism was explored. The removal feasibility of the oil adhered to the MS in a hydrocyclone was also investigated. The oil could be quickly adsorbed by the MS, and the adsorption capacity was positively correlated with oil density. A high flux of 14,436 L·m·h and a considerable separation efficiency of 99% were obtained when the MS was applied for oil-water mixture separation. Additionally, the highest separation efficiency of various emulsions was up to 99.3%. Regrading actual petroleum refinery wastewater, the oil removal efficiency of the MS reached 90% rather than 57.8% of raw sand. The oil droplets in the wastewater were efficiently separated by the MS based on the mechanism of adsorption and coalescence. Additionally, the oil adhered on the MS could be removed, and the oil concentration decreased from 17.6% to 5.2%, which was ascribed to the MS spinning in a hydrocyclone. A novel oil-water separation method of hydrocyclone-intensified filtration by facile and highly hydrophobic sand coating was proposed, and simultaneously the filter media can be effectively regenerated. It is believed that this work might provide a low cost, recyclable and efficient strategy for oil removal, which shows high promise for industrial oily wastewater treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.149488DOI Listing
August 2021

Immune evasion of SARS-CoV-2 from interferon antiviral system.

Comput Struct Biotechnol J 2021 26;19:4217-4225. Epub 2021 Jul 26.

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071/430207, China.

The on-going pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to unprecedented medical and socioeconomic crises. Although the viral pathogenesis remains elusive, deficiency of effective antiviral interferon (IFN) responses upon SARS-CoV-2 infection has been recognized as a hallmark of COVID-19 contributing to the disease pathology and progress. Recently, multiple proteins encoded by SARS-CoV-2 have been shown to act as potential IFN antagonists with diverse possible mechanisms. Here, we summarize and discuss the strategies of SARS-CoV-2 for evasion of innate immunity (particularly the antiviral IFN responses), understanding of which will facilitate not only the elucidation of SARS-CoV-2 infection and pathogenesis but also the development of antiviral intervention therapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.csbj.2021.07.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310780PMC
July 2021

Functional effectiveness of double essential [email protected] starch/microcrystalline cellulose as active antibacterial packaging.

Int J Biol Macromol 2021 Sep 19;186:873-885. Epub 2021 Jul 19.

School of Food and Biological Engineering, Hefei University of Technology, 230009 Hefei, Anhui, PR China; Anhui Institute of Agro-Products Intensive Processing Technology, 230009 Hefei, Anhui, PR China.

In this work, two combinations of double EOs, i.e., α-terpineol: eugenol (α-T:Eu) and carvacrol:eugenol (CA:Eu), are used to develop the active antibacterial films of double [email protected] starch/microcrystalline cellulose ([email protected]). The hydrogen-bonded networks in SC matrix are conducive to thermostability enhancement and the film of SC25 is determined for EO incorporation. The interactions between EOs and SC matrix are also hydrogen bonds and the double [email protected] are smooth at ratio of ≤2:2 for α-T:Eu or CA:Eu. The ultimate film properties are dependent on the incorporated EOs. The release of EOs is well controlled by two mechanisms of diffusion (predominant) and swelling (secondary). Synergetic antibacterial activity occurs on double [email protected] The shelf life of pork can be extended by 1 day at 25 °C by the two typical films of α-T:[email protected] and CA:[email protected] Moreover, [email protected] can be well degraded in humus soil. Thereby, the target films will have great potential in active packaging to extend the shelf life of food.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.07.094DOI Listing
September 2021

Correction to: Establishment of a Reverse Genetic System of Severe Fever with Thrombocytopenia Syndrome Virus Based on a C4 Strain.

Virol Sin 2021 Jul 7. Epub 2021 Jul 7.

State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12250-021-00420-9DOI Listing
July 2021

Crimean-Congo Hemorrhagic Fever Virus: Current Advances and Future Prospects of Antiviral Strategies.

Viruses 2021 06 22;13(7). Epub 2021 Jun 22.

State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

Crimean-Congo hemorrhagic fever virus (CCHFV) is a widespread, tick-borne pathogen that causes Crimean-Congo hemorrhagic fever (CCHF) with high morbidity and mortality. CCHFV is transmitted to humans through tick bites or direct contact with patients or infected animals with viremia. Currently, climate change and globalization have increased the transmission risk of this biosafety level (BSL)-4 virus. The treatment options of CCHFV infection remain limited and there is no FDA-approved vaccine or specific antivirals, which urges the identification of potential therapeutic targets and the design of CCHF therapies with greater effort. In this article, we discuss the current progress and some future directions in the development of antiviral strategies against CCHFV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/v13071195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310003PMC
June 2021

Exosomal ncRNAs profiling of mycobacterial infection identified miRNA-185-5p as a novel biomarker for tuberculosis.

Brief Bioinform 2021 Jun 25. Epub 2021 Jun 25.

Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: There are ever increasing researches implying that noncoded RNAs (ncRNAs) specifically circular RNAs (circRNAs) and microRNAs (miRNAs) in exosomes play vital roles in respiratory disease. However, the detailed mechanisms persist to be unclear in mycobacterial infection.

Methods: In order to detect circRNAs and miRNAs expression pattern and potential biological function in tuberculosis, we performed immense parallel sequencing for exosomal ncRNAs from THP-1-derived macrophages infected by Mycobacterium tuberculosis H37Ra, Mycobacterium bovis BCG and control Streptococcus pneumonia, respectively and uninfected normal cells. Besides, THP-1-derived macrophages were used to verify the validation of differential miRNAs, and monocytes from PBMCs and clinical plasma samples were used to further validate differentially expressed miR-185-5p.

Results: Many exosomal circRNAs and miRNAs associated with tuberculosis infection were recognized. Extensive enrichment analyses were performed to illustrate the major effects of altered ncRNAs expression. Moreover, the miRNA-mRNA and circRNA-miRNA networks were created and expected to reveal their interrelationship. Further, significant differentially expressed miRNAs based on Exo-BCG, Exo-Ra and Exo-Control, were evaluated, and the potential target mRNAs and function were analyzed. Eventually, miR-185-5p was collected as a promising potential biomarker for tuberculosis.

Conclusion: Our findings provide a new vision for exploring biological functions of ncRNAs in mycobacterial infection and screening novel potential biomarkers. To sum up, exosomal ncRNAs might represent useful functional biomarkers in tuberculosis pathogenesis and diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/bib/bbab210DOI Listing
June 2021

Chemical compositions evolution of groundwater and its pollution characterization due to agricultural activities in Yinchuan Plain, northwest China.

Environ Res 2021 09 8;200:111449. Epub 2021 Jun 8.

School of Hydropower & Information Engineering, Huazhong University of Science and Technology, Wuhan, PR China.

Yinchuan Plain is a typically intensive cultivated region in the northwest of China. The irrigation return infiltration from Yellow River is the main source of groundwater recharge. Deep soil layers, sandy vadose zones, and dense irrigation canals make the groundwater susceptible to the return flow which contains pollutants originating mainly from agriculture applications, particularly from the extensive use of nitrogen fertilizer and manure. The pollution levels of phreatic water and confined water in NWS areas (non-water source areas) and WS areas (water source areas) of Yinchuan Plain in 2004 and 2014 were evaluated by the single-factor evaluation method, fuzzy comprehensive evaluation method, and average benchmark coefficient method, respectively. Piper trilinear diagram and scatter plots of major ions were used to classify water types and chemical facies, and further analyze the causes of groundwater pollution and the variation tendency of agricultural pollution. The results show that in 2014, about 50% of the groundwater samples were heavily polluted in Yinchuan Plain, the pollution level of phreatic water and confined water in NWS areas was up to level 5. And the groundwater within the standard in 2004 was heavily polluted in 2014 in WS areas, three-nitrogen pollution was the most serious pollutant, and the organic pollution level was grade IV. From the scatter plots of ions, it can be seen that the increase in concentrations of major ions was affected by evaporation-condensation and cation exchange reaction, but the complex ion contents indicated that groundwater was affected by human activities. The intensive agricultural activities, such as over fertilization, artificial irrigation, have led to concentrations increase of some chemical composition in groundwater.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2021.111449DOI Listing
September 2021

The kinetics and release behaviour of curcumin loaded pH-responsive PLGA/chitosan fibers with antitumor activity against HT-29 cells.

Carbohydr Polym 2021 Aug 15;265:118077. Epub 2021 Apr 15.

School of Chemistry and Chemical Engineering, Hefei University of Technology, 230009, Hefei, Anhui, PR China; Anhui Institute of Agro-Products Intensive Processing Technology, 230009, Hefei, Anhui, PR China. Electronic address:

The bioavailability and clinical effect of curcumin (Cur) are greatly restricted due to its physicochemical instability and high hydrophobicity. To overcome the disadvantages, the nanofibers of poly(lactide-glycolide)/chitosan loaded with Cur (PLGA/CS/Cur) was developed here by electrospinning technique for controlled Cur delivery. The incorporated Cur was well-dispersed and maintained crystalline form in PLGA/CS fiber matrix by hydrogen bonding. The incorporation of Cur had no obvious influence on the fiber size and morphology but exerted impacts on thermal stability. At pH 7.4, the release followed Fickian diffusion mechanism; while at pH 2.0, the release followed the coexistence of diffusion and erosion mechanisms. In addition, the amount of Cur released at pH 2.0 was much higher than that at pH 7.4. As a result, the nanofibers demonstrated higher anticancer activity at acidic environment. Therefore, the PLGA/CS/Cur nanofibers may be served as a potential pH responsive vehicle for the controlled drug delivery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2021.118077DOI Listing
August 2021

Performance of the graphite felt flow-through electrode in hexavalent chromium reduction using a single-pass mode.

J Hazard Mater 2021 Aug 1;416:125768. Epub 2021 Apr 1.

State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, China; National Engineering Laboratory for Industrial Wastewater Treatment, East China University of Science and Technology, Shanghai 200237, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Flow-through electrodes generally outcompete traditional parallel-plate electrodes in current efficiency and mass transfer. High-performance electrode materials can be costly and complicated to fabricate, hindering their wide application. In this study, we used commercial graphite felt (GF) as the cathode of a flow-through electrochemical cell to investigate its potential in treating Cr(VI) solution through electroreduction. The flow-through design with the porous GF electrode allowed sufficient contact surface with Cr(VI) and single-pass tests demonstrated a high reduction efficiency (95~100%) [117 mg/L~3 mg/L Cr(VI)] under acidic conditions. Slow flow rate and high current promoted electroreduction of Cr(VI). The presence of other metal ions could further improve Cr(VI) reduction at low flow rates due to enhanced conductivity in dilute solutions and generation of low valent ions as reducing agents. At fast flow rates, competition of these ions for reduction decreased Cr(VI) reduction efficiency. Moreover, an acidic environment prevented the coating of an insoluble layer on the GF surface and promoted durable performance, with a lower energy consumption [0.46 kWh for treating 100 L 117 mg/L Cr(VI) solution per unit area of GF]. This work demonstrated the potential of Cr(VI) detoxification using GF cathodes in flow-through electrochemical cell.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.125768DOI Listing
August 2021

Influence of oil droplet behavior in electrochemical micromembrane cells on treating oil/water emulsions with low-salt concentrations.

Sci Total Environ 2021 Aug 20;781:146633. Epub 2021 Mar 20.

National Engineering Laboratory for Industrial Wastewater Treatment, East China University of Science and Technology, Shanghai 200237, China; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Although flow-through electrode has demonstrated its potential in treating oily wastewater, few studies noted influence of oil droplet behavior on treating oil/water emulsions. In order to explore the influence of oil droplet behavior in a flow-through electrode cell on treating oil/water emulsions with low-salt concentrations, an electrochemical micromembrane cell was applied to treat oil/water emulsions with 0-0.8 g/L NaCl. High chemical oxygen demand (COD) reduction (80-90%) was obtained in treating Sodium dodecylbenzene sulfonate (SDBS) or Tween 80 emulsion by flow-through electrode, while the later had the higher permeate flux (900 mL/min around). The low salt concentration (0.5 g/L NaCl) achieved high COD reduction (87%) and good permeate flux (600 mL/min). Observations using optical microscopy revealed severe deformation of the shape of the charged oil droplet at the flow-through electrode interface. The wetting of oil droplets at the electrode interface occurred when the membrane acted as an anode, which resulted in flow-through electrode fouling, and subsequently, the reduction in permeate flux and treatment efficiency. The results of this study offer an attractive option when using flow-through electrode to treat oil-in-water emulsions under low-salinity conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.146633DOI Listing
August 2021

Establishment of a Reverse Genetic System of Severe Fever with Thrombocytopenia Syndrome Virus Based on a C4 Strain.

Virol Sin 2021 Mar 15. Epub 2021 Mar 15.

State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China.

Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne bunyavirus that causes hemorrhagic fever-like disease (SFTS) in humans with a case fatality rate up to 30%. To date, the molecular biology involved in SFTSV infection remains obscure. There are seven major genotypes of SFTSV (C1-C4 and J1-J3) and previously a reverse genetic system was established on a C3 strain of SFTSV. Here, we reported successfully establishment of a reverse genetics system based on a SFTSV C4 strain. First, we obtained the 5'- and 3'-terminal untranslated region (UTR) sequences of the Large (L), Medium (M) and Small (S) segments of a laboratory-adapted SFTSV C4 strain through rapid amplification of cDNA ends analysis, and developed functional T7 polymerase-based L-, M- and S-segment minigenome assays. Then, full-length cDNA clones were constructed and infectious SFTSV were recovered from co-transfected cells. Viral infectivity, growth kinetics, and viral protein expression profile of the rescued virus were compared with the laboratory-adapted virus. Focus formation assay showed that the size and morphology of the foci formed by the rescued SFTSV were indistinguishable with the laboratory-adapted virus. However, one-step growth curve and nucleoprotein expression analyses revealed the rescued virus replicated less efficiently than the laboratory-adapted virus. Sequence analysis indicated that the difference may be due to the mutations in the laboratory-adapted strain which are more prone to cell culture. The results help us to understand the molecular biology of SFTSV, and provide a useful tool for developing vaccines and antivirals against SFTS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12250-021-00359-xDOI Listing
March 2021

Systematic Analysis of 42 Autographa Californica Multiple Nucleopolyhedrovirus Genes Identifies An Additional Six Genes Involved in the Production of Infectious Budded Virus.

Virol Sin 2021 Aug 8;36(4):762-773. Epub 2021 Mar 8.

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China.

Baculoviruses have been widely used as a vector for expressing foreign genes. Among numerous baculoviruses, Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the most frequently used and it encodes 155 open reading frames (ORFs). Here, we systematically investigated the impact of 42 genes of AcMNPV on the production of infectious budded viruses (BVs) by constructing gene-knockout bacmids and subsequently conducting transfection and infection assays. The results showed that among the 39 functionally unverified genes and 3 recently reported genes, 36 are dispensable for infectious BV production, as the one-step growth curves of the gene-knockout viruses were not significantly different from those of the parental virus. Three genes (ac62, ac82 and ac106/107) are essential for infectious BV production, as deletions thereof resulted in complete loss of infectivity while the repaired viruses showed no significant difference in comparison to the parental virus. In addition, three genes (ac13, ac51 and ac120) are important but not essential for infectious BV production, as gene-knockout viruses produced significantly lower BV levels than that of the parental virus or repaired viruses. We then grouped the 155 AcMNPV genes into three categories (Dispensable, Essential, or Important for infectious BV production). Based on our results and previous publications, we constructed a schematic diagram of a potential mini-genome of AcMNPV, which contains only essential and important genes. The results shed light on our understanding of functional genomics of baculoviruses and provide fundamental information for future engineering of baculovirus expression system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12250-021-00355-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379328PMC
August 2021

A review of treatment technologies for produced water in offshore oil and gas fields.

Sci Total Environ 2021 Jun 30;775:145485. Epub 2021 Jan 30.

School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address:

Offshore oil and gas production is increasingly growing popular globally. Produced water (PW), which is the largest byproduct of oil and gas production, is a complex mixture of dissolved and undissolved organic and inorganic substances. PW contributes considerably to oil pollution in the offshore petroleum and gas industry owing to the organic substances, which mainly include hydrocarbons; this is a major concern to researchers because of the long-term adverse effects on the ecosystem. Since the development of offshore petroleum and gas industry, the PW treatment process has been classified into pretreatment, standard-reaching treatment, and advanced purification treatment based on the characteristics of PW and has been coupled with the environmental, economic, and regulatory considerations. The mechanism, design principle, application, and development of conventional technologies for PW treatment, such as gravity and enhanced gravity sedimentation, hydrocyclone, gas flotation, and medium filtration, are summarized in this study. Novel methods for further application, such as tubular separation, combined fibers coalescence, and membrane separation, are also discussed. Enhancement of treatment with multiple physical fields and environmentally friendly chemical agents, coupled with information control technology, would be the preferred PW treatment approach in the future. Moreover, the PW treatment system should be green, efficient, secure, and intelligent to satisfy the large-scale, unmanned, and abyssal exploration of offshore oil and gas production in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.145485DOI Listing
June 2021

Fiber coalescence treatment of oily wastewater: A new theory and application.

J Hazard Mater 2021 06 21;412:125188. Epub 2021 Jan 21.

National Engineering Laboratory for Industrial Wastewater Treatment, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address:

Oil pollution from produced water in the offshore petroleum industry is one of the most serious marine pollutants worldwide, and efficient separation technology is crucial for the control of oil pollutant emission. Medium coalescence is an efficient oil-water separation technology, but its theory is lacking and the development is slow. In this work, the microscopic mechanism of fiber coalescence was revealed, and found that the effective collision positions were the three-phase contact line and the exposed fiber surface. Further, a theoretical model for calculating the separation performance of a fiber bed was established. For a given inlet droplet size distribution and bed geometric parameters, the outlet droplet size distribution and the total separation efficiency of the fiber bed can be predicted. Then, an Ω-shaped woven method composed of oil-wet fibers and oil-phobic fibers was designed and the separation performance of the fiber beds prepared by the method and the influence law of various parameters were clarified through macroscopic experiment. Finally, the novel technology achieved its first engineering application on an offshore platform, with the average oil content of the outlet was less than 25 mg/L, which could reform the current treatment process of produced water.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.125188DOI Listing
June 2021

Systematic analysis of nuclear localization of Autographa californica multiple nucleopolyhedrovirus proteins.

J Gen Virol 2021 03 18;102(3). Epub 2021 Jan 18.

University of Chinese Academy of Sciences, Beijing 100049, PR China.

Baculoviruses are large DNA viruses that replicate within the nucleus of infected host cells. Therefore, many viral proteins must gain access to the nucleus for efficient viral genome replication, gene transcription and virion assembly. To date, the global protein localization pattern of baculoviral proteins is unknown. In this study, we systematically analysed the nuclear localization of 154 ORFs encoded by the prototypic baculovirus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), either during transient expression or with super-infection of the virus. By transient expression of vectors containing -fused ORFs, we found that in the absence of virus infection, 25 viral proteins were localized in the nucleus. Most of these, which we called 'auto-nuclear localization' proteins, are related to virus replication, transcription or virion structure, and 20 of them contain predicted classical nuclear localization signal. Upon virus infection, 11 proteins, which originally localized in the cytoplasm or both cytoplasm and nucleus in the transfection assays, were completely translocated into the nucleus, suggesting that their nuclear import is facilitated by other viral or host proteins. Further co-transfection experiments identified that four of the 11 proteins, including P143, P33, AC73 and AC114, were imported into the nucleus with the assistance of the auto-nuclear localization proteins LEF-3 (for P143), TLP (for P33) and VP80 (for both AC73 and AC114). This study presents the first global nuclear localization profile of AcMNPV proteins and provides useful information for further elucidation of the mechanisms of baculovirus nuclear entry and gene functions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/jgv.0.001517DOI Listing
March 2021

Antibacterial Activity of Polyvinyl Alcohol/WO Films Assisted by Near-Infrared Light and Its Application in Freshness Monitoring.

J Agric Food Chem 2021 Jan 15;69(3):1068-1078. Epub 2021 Jan 15.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009 Anhui, P. R. China.

Nowadays, films with antibacterial activity and applied for freshness monitoring by colorimetric response have been drawing growing attention in food packaging. However, the development of versatile antibacterial and colorimetric agents is still highly desirable. Herein, WO nanorods are incorporated in a polyvinyl alcohol (PVA) matrix to develop a novel composite film with photothermal antibacterial activity and freshness monitoring faculty. The interaction between WO nanorods and PVA is due to hydrogen bonds. Compared with the PVA film, the presence of WO nanorods can significantly enhance the mechanical and barrier properties; typically, the target film (WO/PVA) shows an increase in tensile strength by 52.7% and Young's modulus by 400.0% and a decrease in oxygen permeability by 72.4% and water vapor permeability by 66.9%. The films demonstrate a WO content-dependent antibacterial activity. Under irradiation of near-infrared light (NIR808), the synergistic effect of physical damage, oxidative stress, and temperature increase markedly improves the antibacterial activity of (WO/PVA), showing an antibacterial efficiency of ∼90% against or beyond 90% against . The incorporated WO nanorods demonstrate lower cytotoxicity toward the model cells of human colon cancer cell line HT-29. The (WO/PVA) film exhibits colorimetric response to HS and can also be used for pork freshness monitoring as an indicator.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.0c06961DOI Listing
January 2021

Host restriction of emerging high-pathogenic bunyaviruses via MOV10 by targeting viral nucleoprotein and blocking ribonucleoprotein assembly.

PLoS Pathog 2020 12 7;16(12):e1009129. Epub 2020 Dec 7.

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.

Bunyavirus ribonucleoprotein (RNP) that is assembled by polymerized nucleoproteins (N) coating a viral RNA and associating with a viral polymerase can be both the RNA synthesis machinery and the structural core of virions. Bunyaviral N and RNP thus could be assailable targets for host antiviral defense; however, it remains unclear which and how host factors target N/RNP to restrict bunyaviral infection. By mass spectrometry and protein-interaction analyses, we here show that host protein MOV10 targets the N proteins encoded by a group of emerging high-pathogenic representatives of bunyaviruses including severe fever with thrombocytopenia syndrome virus (SFTSV), one of the most dangerous pathogens listed by World Health Organization, in RNA-independent manner. MOV10 that was further shown to be induced specifically by SFTSV and related bunyaviruses in turn inhibits the bunyaviral replication in infected cells in series of loss/gain-of-function assays. Moreover, animal infection experiments with MOV10 knockdown corroborated the role of MOV10 in restricting SFTSV infection and pathogenicity in vivo. Minigenome assays and additional functional and mechanistic investigations demonstrate that the anti-bunyavirus activity of MOV10 is likely achieved by direct impact on viral RNP machinery but independent of its helicase activity and the cellular interferon pathway. Indeed, by its N-terminus, MOV10 binds to a protruding N-arm domain of N consisting of only 34 amino acids but proving important for N function and blocks N polymerization, N-RNA binding, and N-polymerase interaction, disabling RNP assembly. This study not only advances the understanding of bunyaviral replication and host restriction mechanisms but also presents novel paradigms for both direct antiviral action of MOV10 and host targeting of viral RNP machinery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.ppat.1009129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746268PMC
December 2020

The Impact of Peroxymonocarbonate (HCO ) on the Transformation of Organic Contaminants during Hydrogen Peroxide (HO) Chemical Oxidation (ISCO).

Environ Sci Technol Lett 2019 Dec 12;6(12):781-786. Epub 2019 Nov 12.

Department of Civil and Environmental Engineering, University of California, Berkeley, Berkeley, CA 94720-1716, USA.

Under the conditions employed when in situ chemical oxidation is used for contaminant remediation, high concentrations of HO (e.g., up to ~10 M) are typically present. Using C NMR, we show that in carbonate-rich systems, these high concentrations of HO result in a reaction with HCO to produce peroxymonocarbonate (HCO ). After formation, HCO reacts with phenol to produce di- and tri-hydroxyl phenols. HCO reacts with substituted phenols in a manner consistent with its electrophilic character. Exchanging an electron-donating substituent in the position of a phenolic compound with an electron-withdrawing group decreased the reaction rate. Results of this study indicate that HCO is a potentially important but previously unrecognized oxidative species generated during HO Chemical Oxidation (ISCO) that selectively reacts with electron-rich organic compounds. Under conditions in which HO· formation is inefficient (e.g., relatively high concentration of HCO , low total Fe and Mn concentrations), the fraction of the phenolic compounds that are transformed by HCO could be similar to or greater than the fraction transformed by HO·. It may be possible to adjust treatment conditions to enhance the formation of HCO as a means of accelerating rates of contaminant removal.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.estlett.9b00682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643861PMC
December 2019

SARS-CoV-2 nsp1: Bioinformatics, Potential Structural and Functional Features, and Implications for Drug/Vaccine Designs.

Front Microbiol 2020 29;11:587317. Epub 2020 Sep 29.

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.

The emerging coronavirus disease (COVID-19) caused by SARS-CoV-2 has led to social and economic disruption globally. It is urgently needed to understand the structure and function of the viral proteins for understanding of the viral infection and pathogenesis and development of prophylaxis and treatment strategies. Coronavirus non-structural protein 1 (nsp1) is a notable virulence factor with versatile roles in virus-host interactions and exhibits unique characteristics on sequence, structure, and function mode. However, the roles and characteristics of SARS-CoV-2 nsp1 are currently unclear. Here, we analyze the nsp1 of SARS-CoV-2 from the following perspectives: (1) bioinformatics analysis reveals that the novel nsp1 is conserved among SARS-CoV-2 strains and shares significant sequence identity with SARS-CoV nsp1; (2) structure modeling shows a 3D α/β-fold of SARS-CoV-2 nsp1 highly similar to that of the SARS-CoV homolog; (3) by detailed, functional review of nsp1 proteins from other coronaviruses (especially SARS-CoV) and comparison of the protein sequence and structure, we further analyzed the potential roles of SARS-CoV-2 nsp1 in manipulating host mRNA translation, antiviral innate immunity and inflammation response and thus likely promoting viral infection and pathogenesis, which are merited to be tested in the future. Finally, we discussed how understanding of the novel nsp1 may provide valuable insights into the designs of drugs and vaccines against the unprecedented coronavirus pandemic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.587317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550470PMC
September 2020

Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes.

Virol Sin 2021 Apr 22;36(2):300-310. Epub 2020 Sep 22.

State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Severe fever with thrombocytopenia syndrome virus (SFTSV), the causative agent of a febrile human disease, was first identified from central and eastern provinces in China, and later in Japan and South Korea. Hubei Province is one of the major SFTS epidemic areas in the central part of China. This study reported the isolation of 11 new SFTSV strains from patients in Hubei Province collected in 2017. Extensive phylogenetic analyses were conducted based on the complete coding sequences of SFTSV segments including the new strains. It was suggested that five different SFTSV genotypes were circulating in Hubei, and 15 reassortment patterns and migration pathways correlated with each genotype were identified, which was more than previously recognized. Hubei Province was more involved in the evolutionary events of SFTSV than that previously thought in which the evolutionary events of SFTSV were reported to be independent from those in other epidemic regions. Further divergence of SFTSV strains was suggested by pairwise comparison of SFTSV sequences from each genotype and sequence identity normalized to representative strain in genotype C1. Subsequently, amino acid variations specific for genotype(s), strain(s), or cluster(s) were inspected, which may be related to differential biological activity of SFTSV strains/genotypes. In conclusion, we analyzed the current status of SFTSV phylogeny in Hubei Province and discussed the possible events correlated to SFTSV evolution. It provided an in-depth insight into SFTSV evolution, raising concerns for the use of proper SFTSV strains in future studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12250-020-00289-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087752PMC
April 2021

N-3 PUFAs inhibited hepatic ER stress induced by feeding of a high-saturated fat diet accompanied by the expression LOX-1.

J Nutr Biochem 2021 02 25;88:108481. Epub 2020 Aug 25.

Hubei Province Engineering Research Center of Healthy Food, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address:

Excessive consumption of saturated fat leads to non-alcoholic fatty liver disease (NAFLD), which is attenuated by supplementation of n-3 polyunsaturated fatty acids (PUFAs). Endoplasmic reticulum (ER) stress is crucial in the development of NAFLD, but how high-saturated fat diet (HFD) causes ER stress and NAFLD remains unclear. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is involved in hepatic ER stress. We aimed to explore the roles of LOX-1 in HFD-induced ER stress. Male Sprague-Dawley rats were fed an HFD without or with supplementation of fish oil for 16 weeks. The effects of n-3 PUFAs on hepatic ER stress degrees and the expression levels of LOX-1 were examined. Then human L02 hepatoma cells were treated with palmitate or palmitate and DHA to determine the ER stress and LOX-1 expression levels in vitro. After that the expression of LOX-1 in L02 cells was either knocked-down or overexpressed to analyze the roles of LOX-1 in palmitate-induced ER stress. The feeding of HFD induced NAFLD development and ER stress in the liver, and LOX-1 expressing level, which were all reversed by fish oil supplementation. In vitro, DHA treatment reduced the expression of LOX-1, and palmitate-induced ER stress. SiRNA-mediated knock-down of LOX-1 inhibited palmitate-induced ER stress, whereas overexpression of LOX-1 dramatically induced ER stress in L02 cells.LOX-1 is critical for HFD-induced ER stress, and inhibition of its expression under the treatment of n-3 PUFAs could ameliorate HFD-induced NAFLD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jnutbio.2020.108481DOI Listing
February 2021

Synergistic antibacterial activity of streptomycin sulfate loaded PEG-MoS/rGO nanoflakes assisted with near-infrared.

Mater Sci Eng C Mater Biol Appl 2020 Nov 20;116:111221. Epub 2020 Jun 20.

School of Food and Biological Engineering, Hefei University of Technology, 230009 Hefei, Anhui, PR China; Anhui Institute of Agro-Products Intensive Processing Technology, 230009, Hefei, Anhui, PR China.

Synergistic antibacterial strategies have received growing attention due to their significantly enhanced antibacterial activity. Herein, we demonstrated a synergistic antibacterial strategy based on streptomycin sulfate (SS) loaded polyethylene glycol-MoS/reduced graphene oxide (PEG-MoS/rGO) nanoflakes assisted with near-infrared (NIR). The nanoflakes of PEG-MoS/rGO were ultrasonically exfoliated well from the nanoflowers of PEG-MoS/rGO fabricated by hydrothermal method, which was of antibacterial activity against Staphylococcus aureus and Escherichia coli after loading of SS. Under the irradiation of NIR, the antibacterial activity was significantly enhanced by the synergistic effects of physical damage, protein synthesis inhibition, thermal injury and oxidative stress. Moreover, the cytotoxicity of the nanoflakes was very low. The results suggest the great potential of PEG-MoS/rGO-SS as a photothermal antibacterial agent.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.msec.2020.111221DOI Listing
November 2020

Immunological detection of serum antibodies in pediatric medical workers exposed to varying levels of SARS-CoV-2.

J Infect 2021 01 25;82(1):159-198. Epub 2020 Jul 25.

State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jinf.2020.07.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382336PMC
January 2021

Identification, Isolation, and Characterization of an Ectromelia Virus New Strain from an Experimental Mouse.

Virol Sin 2021 Feb 21;36(1):155-158. Epub 2020 Jul 21.

State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12250-020-00263-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973332PMC
February 2021

A RIG-I-like receptor directs antiviral responses to a bunyavirus and is antagonized by virus-induced blockade of TRIM25-mediated ubiquitination.

J Biol Chem 2020 07 29;295(28):9691-9711. Epub 2020 May 29.

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China

The RIG-I-like receptors (RLRs) retinoic acid-inducible gene I protein (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) are cytosolic pattern recognition receptors that recognize specific viral RNA products and initiate antiviral innate immunity. Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic member of the RIG-I, but not MDA5, has been suggested to sense some bunyavirus infections; however, the roles of RLRs in anti-SFTSV immune responses remain unclear. Here, we show that SFTSV infection induces an antiviral response accompanied by significant induction of antiviral and inflammatory cytokines and that RIG-I plays a main role in this induction by recognizing viral 5'-triphosphorylated RNAs and by signaling via the adaptor mitochondrial antiviral signaling protein. Moreover, MDA5 may also sense SFTSV infection and contribute to IFN induction, but to a lesser extent. We further demonstrate that the RLR-mediated anti-SFTSV signaling can be antagonized by SFTSV nonstructural protein (NSs) at the level of RIG-I activation. Protein interaction and MS-based analyses revealed that NSs interacts with the host protein tripartite motif-containing 25 (TRIM25), a critical RIG-I-activating ubiquitin E3 ligase, but not with RIG-I or Riplet, another E3 ligase required for RIG-I ubiquitination. NSs specifically trapped TRIM25 into viral inclusion bodies and inhibited TRIM25-mediated RIG-I-Lys-63-linked ubiquitination/activation, contributing to suppression of RLR-mediated antiviral signaling at its initial stage. These results provide insights into immune responses to SFTSV infection and clarify a mechanism of the viral immune evasion, which may help inform the development of antiviral therapeutics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1074/jbc.RA120.013973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363118PMC
July 2020

Infectivity Factor 5 Identified as a Substrate of P33 in the Baculoviral Disulfide Bond Formation Pathway.

J Virol 2020 07 16;94(15). Epub 2020 Jul 16.

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People's Republic of China

Disulfide bonds are critical for the structure and function of many proteins. Some large DNA viruses encode their own sulfhydryl oxidase for disulfide bond formation. Previous studies have demonstrated that the baculovirus-encoded sulfhydryl oxidase P33 is necessary for progeny virus production, and its enzymatic activity is important for morphogenesis and oral infectivity of baculoviruses. However, the downstream substrates of P33 in the putative redox pathway of baculoviruses are unknown. In this study, we showed that PIF5, one of the infectivity factors (PIFs), contained intramolecular disulfide bonds and that the disulfide bond formation was interrupted in the absence of P33. pulldown and colocalization analyses revealed that PIF5 and P33 interacted with each other during virus infection. Further, assays validated that the reduced PIF5 proteins could be oxidized by P33. To understand the contribution of disulfide bonds to the function of PIF5, several cysteine-to-serine mutants were constructed, which all interfered with the disulfide bond formation of PIF5 to different extents. All the mutants lost their oral infectivity but had no impact on infectious budding virus (BV) production or virus morphogenesis. Taken together, our results indicated PIF5 as the first identified substrate of P33. Further, the disulfide bonds in PIF5 play an essential role in its function in oral infection. Similar to some large DNA viruses that encode their own disulfide bond pathway, baculovirus encodes a viral sulfhydryl oxidase, P33. Enzyme activity of P33 is related to infectious BV production, occlusion-derived virus (ODV) envelopment, occlusion body morphogenesis, and oral infectivity, suggesting that P33 is involved in disulfide bond formation of multiple proteins. A complete disulfide bond formation pathway normally contains a sulfhydryl oxidase, a disulfide-donating enzyme, and one or more substrates. In baculovirus, apart from P33, other components of the putative pathway remain unknown. In this study, we identified PIF5 as the first substrate of P33, which is fundamental for revealing the complete disulfide bond formation pathway in baculovirus. PIF5 is essential for oral infection and is absent from the PIF complex. Our study demonstrated that native disulfide bonds in PIF5 are required for oral infection, which will help us to reveal its mode of action.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/JVI.00615-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7375385PMC
July 2020

Combinatorial Minigenome Systems for Emerging Banyangviruses Reveal Viral Reassortment Potential and Importance of a Protruding Nucleotide in Genome "Panhandle" for Promoter Activity and Reassortment.

Front Microbiol 2020 8;11:599. Epub 2020 Apr 8.

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.

is a new genus ( family, order) that comprises a group of emerging tick-borne viruses with severe fever with thrombocytopenia syndrome virus (SFTSV) and Heartland virus (HRTV) as virulent representatives. As segmented RNA viruses, bunyaviruses may have genome reassortment potential, increasing the concern about new life-threatening bunyavirus emergence. Using a series of combinatory minigenome reporter assays based on transfection and superinfection, we showed that replication machinery proteins of designated banyangviruses can recognize genomic untranslated regions (UTRs) of other banyangviruses and assemble heterogenous minigenomes into functional ribonucleoproteins (RNPs). Moreover, both heterogenous and heterozygous RNPs were efficiently packaged by viral glycoproteins into infectious virus-like particles, manifesting remarkable reassortment potential of banyangviruses. Meanwhile, UTR promoter strength of the three banyangvirus segments appeared to be M > L > S. Secondary structure analysis revealed a conservative non-basepairing protruding nucleotide in the terminal UTR panhandles of M and L (but not S) segments of all banyangviruses and some related phleboviruses ( genus). Furthermore, not only a conserved panhandle region but also the protruding nucleotide proved important for UTR function. Removal of the protruding nucleotide abated M and L UTR activities and compatibilities with heterogenous viral proteins, and introduction of a protruding nucleotide into S panhandle, conversely, enhanced UTR promoter strength and compatibility, revealing the significance of the protruding nucleotide as a new signature of the genomic panhandle structure in both UTR activity and reassortment potential. The study demonstrates not only banyangvirus reassortment potential but also the notable role of the protruding nucleotide in UTR function and reassortment, providing clues to viral evolution and replication mechanisms and perhaps benefiting disease control and prevention in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.00599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156889PMC
April 2020

DHA substitution overcomes high-fat diet-induced disturbance in the circadian rhythm of lipid metabolism.

Food Funct 2020 Apr 15;11(4):3621-3631. Epub 2020 Apr 15.

Hubei Province Engineering Research Center of Healthy Food, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China.

Disruptions to circadian rhythm have been associated with an increased risk of nonalcoholic fatty liver disease (NAFLD). DHA has been found to affect both circadian rhythm and lipid metabolism. In this study, the relationship between DHA substitution and improvements in lipid metabolism and circadian clock regulation was studied. Male C57BL/6 mice were fed a control, a high fat or a DHA substituted diet for 12 weeks. Biochemical analysis and H&E staining showed that the high-fat diet (HFD) could induce NAFLD, and DHA substitution (AOH) could attenuate NAFLD. The qPCR results showed that the expressions of core clock genes Clock and Bmal1 were significantly higher at zeitgeber (ZT) 0 (7:00 am) than those at ZT12 (7:00 pm) in the control group, while this difference in day and night disappeared in the HFD group, but was observed in the AOH group. Western blotting results indicated that the expressions of rhythm output molecules (RORα and REV-ERBα) and their downstream protein INSIG2 all showed the corresponding circadian changes. SREBP-regulated proteins were significantly increased in the HFD group at both ZT0 and ZT12, but decreased in the AOH group accompanied by the corresponding changes in the protein expressions of HMGCR, LXR, CYP7A1 and CYP27A1. Altogether, HFD can decrease or disrupt circadian rhythm fluctuation by up-regulating the expression of core circadian rhythm genes Clock and Bmal1 at ZT12, and induce metabolic abnormalities through the INSIG2-SREBP pathway regulated by RORα and REV-ERBα. DHA substitution seems to restore circadian rhythm similar to the normal circadian rhythm of "night-high, day-low" through the metabolic pathway regulated by rhythmic nuclear receptors, improving the lipid metabolism rhythm and reducing liver fat.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9fo02606aDOI Listing
April 2020
-->