Publications by authors named "Huaixin Wang"

7 Publications

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Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF-κB pathway.

Exp Ther Med 2021 Nov 16;22(5):1309. Epub 2021 Sep 16.

Department of Emergency Medicine, Yidu Central Hospital of Weifang, Weifang, Shandong 262500, P.R. China.

Atherosclerosis is a chronic inflammatory disease associated with inflammatory responses and the uncontrolled proliferation and excessive apoptosis of vascular smooth muscle cells. However, the effects of matrine on the inflammatory response, abnormal lipid metabolism and cell proliferation and apoptosis marker proteins in human aortic vascular smooth muscle cells (HAVSMCs) have not been elucidated. Therefore, the present study aimed to investigate the effect of matrine on an model of atherosclerosis using HAVSMCs. The HAVSMCs were divided into normal, model and matrine groups. The model group was treated with oxidized low-density lipoprotein (oxLDL), the matrine group was treated with oxLDL and matrine and the normal group was treated with physiological saline. Total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) levels were measured in the cell supernatant. In addition, the relative mRNA levels of inflammatory factors were quantified using reverse transcription-quantitative PCR, and the cell proliferation and apoptosis rates were evaluated using Cell Counting Kit-8 and flow cytometry assays, respectively. The expression levels of proteins associated with proliferation and apoptosis were also determined using western blotting. The levels of TC, FC and CE and the mRNA levels of IL-1β, IL-6, and TNF-α in the matrine group were lower than those in the model group, but higher than those in the normal group. After 48 and 96 h of treatment, the cell proliferation and apoptotic rates were lower in the matrine group compared with the model group. The relative expression levels of Ki-67, proliferating cell nuclear antigen and Bax were decreased, while that of Bcl-2 was increased in the matrine group compared with the model group. In addition, the relative protein expression of nuclear factor κB (NF-κB) in the matrine group was lower than that in the model group, but higher than that in the normal group. In summary, matrine inhibited activation of the NF-κB pathway and reduced cell proliferation and apoptosis in the oxLDL-induced atherosclerosis model, and exhibited anti-inflammatory effects. These results suggest that matrine attenuated abnormal biological reactions in HAVSMCs through the NF-κB pathway.
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http://dx.doi.org/10.3892/etm.2021.10744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461614PMC
November 2021

Diagnostic value and prognostic significance of CTRP9 combined with pentraxin-3 in acute coronary syndrome.

Exp Ther Med 2021 Mar 25;21(3):254. Epub 2021 Jan 25.

Department of Cardiology, Dongying Hospital of Traditional Chinese Medicine, Dongying, Shandong 257000, P.R. China.

The present study aimed to explore the diagnostic value and prognostic significance of C1q/tumor necrosis factor-related protein 9 (CTRP9) combined with pentraxin-3 (PTX-3) in acute coronary syndrome (ACS). A total of 137 patients with coronary heart disease and chest pain were included. Among them, seventy-nine patients with ACS were allocated into a study group and fifty-eight patients with non-cardiac chest pain (NCCP) were allocated into a control group. The serum CTRP9, PTX-3 levels were quantified by ELISA, and their correlation with other ACS-related indexes, diagnostic value for ACS and predictive significance for poor prognosis were analyzed. In addition, the risk factors of the poor prognosis of ACS patients were studied. CTRP9 was lowly expressed and PTX-3 was highly expressed in the serum of ACS patients. CTRP9 was negatively correlated with cardiac troponin I (cTnI), creatine kinase-MB (CK-MB) and high-sensitivity C-reactive protein (hs-CRP) (P<0.05), while PTX-3 was positively correlated with them (P<0.05). Combined detection of CTRP9 and PTX-3 was of high value in the diagnosis and prognosis of ACS patients. In addition, CTRP9 and PTX-3 were independent risk factors for the poor prognosis of ACS. Patients with ACS had lower CTRP9 expression and higher PTX-3 expression than those without ACS. Moreover, the combined detection of CTRP9 and PTX-3 can better evaluate the diagnosis and prognosis of ACS patients.
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http://dx.doi.org/10.3892/etm.2021.9685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851676PMC
March 2021

Characterization of the mitochondrial genome of (Cypriniformes) and its taxonomic status.

Mitochondrial DNA B Resour 2020 18;5(3):2101-2102. Epub 2020 May 18.

College of Animal Science, Guizhou University, Guiyang, P.R. China.

In the present study, the complete mitochondrial genome of has been amplified with 16 pairs of primers. There are 16 627 base pairs has been identified and deposited in the GenBank with accession numbers MN970213. The arrangement was similar to typical vertebrate mitochondrial, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding control region. The overall base composition of was G + C: 42.9%, A + T: 57.1%, apparently with a slight AT bias. Phylogenetic analysis showed that was close to .
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http://dx.doi.org/10.1080/23802359.2020.1763215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510660PMC
May 2020

A Meta-Analysis about the Screening Role of Pulse Oximetry for Congenital Heart Disease.

Biomed Res Int 2017 10;2017:2123918. Epub 2017 Dec 10.

Emergency Department, Yidu Central Affiliated Hospital of Weifang Medical University, 4138 Linglong South Road, Qingzhou, Shandong Province 262550, China.

Objective: The opinions about the application of pulse oximetry in diagnosis of congenital heart disease (CHD) were debatable. We performed this meta-analysis to confirm the diagnostic role of pulse oximetry screening for CHD.

Methods: Relevant articles were searched in the databases of Pubmed, Embase, Google Scholar, and Chinese National Knowledge Infrastructure (CNKI) up to April 2017. Data was processed in the MetaDiSc 1.4 software. Pooled sensitivity and specificity with 95% confidence interval (95% CI) were calculated to explain the diagnostic role of pulse oximetry screening for CHD. ⩾50% or < 0.05 indicated significant heterogeneity. Area under curve (AUC) of summary receiver operating characteristics (SROC) was calculated to assess its diagnostic accuracy. The robustness of overall results was evaluated by sensitivity analysis. Publication bias was evaluated by Deek's funnel plot.

Results: 22 eligible articles were selected. Pooled sensitivity and specificity were 0.69 (0.67-0.72) and 0.99 (0.99-0.99), respectively. The corresponding AUC was 0.9407, suggesting high diagnostic accuracy of pulse oximetry screening for CHD. Sensitivity analysis demonstrated that the pooled results were robust. Deek's funnel plot seemed to be symmetrical.

Conclusions: Pulse oximetry screening could be used to diagnose CHD. It shows high diagnosis specificity and accuracy.
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http://dx.doi.org/10.1155/2017/2123918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742437PMC
August 2018

Creation of antifouling microarrays by photopolymerization of zwitterionic compounds for protein assay and cell patterning.

Biosens Bioelectron 2018 Apr 7;102:63-69. Epub 2017 Nov 7.

School of Marine Science & Technology, Harbin Institute of Technology at Weihai, Weihai, Shandong Province 264209, PR China. Electronic address:

Nonspecific binding or adsorption of biomolecules presents as a major obstacle to higher sensitivity, specificity and reproducibility in microarray technology. We report herein a method to fabricate antifouling microarray via photopolymerization of biomimetic betaine compounds. In brief, carboxybetaine methacrylate was polymerized as arrays for protein sensing, while sulfobetaine methacrylate was polymerized as background. With the abundant carboxyl groups on array surfaces and zwitterionic polymers on the entire surfaces, this microarray allows biomolecular immobilization and recognition with low nonspecific interactions due to its antifouling property. Therefore, low concentration of target molecules can be captured and detected by this microarray. It was proved that a concentration of 10ngmL bovine serum albumin in the sample matrix of bovine serum can be detected by the microarray derivatized with anti-bovine serum albumin. Moreover, with proper hydrophilic-hydrophobic designs, this approach can be applied to fabricate surface-tension droplet arrays, which allows surface-directed cell adhesion and growth. These light controllable approaches constitute a clear improvement in the design of antifouling interfaces, which may lead to greater flexibility in the development of interfacial architectures and wider application in blood contact microdevices.
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http://dx.doi.org/10.1016/j.bios.2017.11.001DOI Listing
April 2018

Affinity Monolith-Integrated Microchips for Protein Purification and Concentration.

Methods Mol Biol 2016 ;1466:85-92

School of Marine Science and Technology, Harbin Institute of Technology at Weihai, 2 West Wenhua Road, Shandong, 264209, China.

Affinity chromatography is a valuable method to purify and concentrate minute amount of proteins. Monoliths with epoxy groups for affinity immobilization were prepared by direct in-situ photopolymerization of glycidyl methacrylate and ethylene glycol dimethacrylate in porogenic solvents consisting of 1-dodecanol and cyclohexanol. By integrating affinity monoliths onto a microfluidic system, targeted biomolecules can be captured and retained on affinity column, while other biomolecules having no specific interactions toward the immobilized ligands flow through the microchannel. Therefore, proteins which remain on the affinity column are purified and concentrated, and then eluted by appropriate solutions and finally, separated by microchip capillary electrophoresis. This integrated microfluidic device has been applied to the purification and separation of specific proteins (FITC-labeled human serum albumin and IgG) in a mixture.
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http://dx.doi.org/10.1007/978-1-4939-4014-1_7DOI Listing
January 2018

[A case report of coronary stenting induced psychiatric symptoms].

Zhonghua Xin Xue Guan Bing Za Zhi 2014 Jan;42(1):62-3

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January 2014
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