Publications by authors named "Huafeng Liu"

127 Publications

miR-584 and miR-146 are candidate biomarkers for acute respiratory distress syndrome.

Exp Ther Med 2021 May 1;21(5):445. Epub 2021 Mar 1.

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China.

MicroRNAs (miRNAs/miRs) have important roles in inflammation and infections, which are common manifestations of acute respiratory distress syndrome (ARDS). The present study aimed to assess whether serum miRNAs are potential diagnostic biomarkers for human ARDS. For this, two sets of serum samples from healthy individuals and patients with ARDS were analysed by high-throughput sequencing to identify differentially expressed genes in ARDS. A total of 679 valid sequences were identified as differentially expressed (P<0.05). Of these, five differentially expressed miRNAs were subjected to reverse transcription-quantitative PCR validation. Finally, two miRNAs (miR-584 and miR-146a) were successfully verified. These two miRNAs were significantly downregulated in the serum of patients with ARDS. Gene Ontology annotations and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that their target transcripts were implicated in a broad range of biological processes and various metabolic pathways, including involvement in the regulation of various inflammatory factors. The present study provided a framework for understanding the molecular mechanisms of ARDS and suggested that miR-584 and miR-146a are associated with ARDS and may be potential therapeutic targets.
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http://dx.doi.org/10.3892/etm.2021.9873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967848PMC
May 2021

Rapid transport of organochlorine pesticides (OCPs) in multimedia environment from karst area.

Sci Total Environ 2021 Jun 6;775:145698. Epub 2021 Feb 6.

State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; School of Environmental Studies, China University of Geosciences, Wuhan 430078, China; Institute of Geological Survey, China University of Geosciences, Wuhan 430074, China; Key Laboratory of Karst Dynamics, MNR & Guangxi, Institute of Karst Geology, CAGS, Guilin 541004, China; State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430078, China; Hubei Provincial Engineering Research Center of Systematic Water Pollution Control, China University of Geosciences, Wuhan 430078, China; Ecological Environment Monitoring Station, Ninth Division, Xinjiang Production and Construction Corps, Tacheng, Xinjiang 834601, China. Electronic address:

Karst groundwater is crucial, but particularly vulnerable to contaminants. Anthropologically derived pollutants on the surface-environment in karst areas could easily and rapidly enter groundwater through highly developed transmissible structures and threaten water safety. To investigate such transport, we analyzed 24 organochlorine pesticides (OCPs) in the multimedia environment from the Zigui karst area of China, where agriculture is the predominant human activity. OCPs were frequently detected with the total OCP concentrations ranged from 228 to 7970 pg/g, 300 to 32,200 pg/L, 318 to 2250 pg/L, 149 to 2760 pg/g, and 752 to 12,000 pg/g in the soil, spring water, river water, spring sediment, and river sediment, respectively. HCB and p,p'-DDT were the most dominant OCP species. Isomeric and metabolic ratios indicated fresh inputs of Lindane, technical DDT, and Aldrin, although they have been banned in China. The spatial distributions, correlation analysis, and regression analysis suggested rapid OCP transport from the soil to the spring water, and from the soil and spring water to river water. OCPs in the soil and springs explained 92.3% and 89.0% of those in the spring water and river water, respectively. The solid transport with the fast-moving water was predominant for OCPs in sediments. Highly dynamic water systems and rapid OCP transport in the intro- and inter-medium suggested by our results substantiate the groundwater's vulnerability in karst areas. More studies on levels and transport of organic contaminants in karst systems and policy for protecting the karst groundwater are urgently required to control contaminant sources and ensure groundwater sustainability, since the karst water resources may suffer a potentially bleak future consisted of the decreased groundwater quantity and low water quality.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145698DOI Listing
June 2021

Lansoprazole promotes cisplatin-induced acute kidney injury via enhancing tubular necroptosis.

J Cell Mol Med 2021 Mar 18;25(5):2703-2713. Epub 2021 Feb 18.

Key Laboratory of Prevention and Management of Chronic Kidney Disease of Zhanjiang City, Institute of Nephrology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Acute kidney injury (AKI) is the main obstacle that limits the use of cisplatin in cancer treatment. Proton pump inhibitors (PPIs), the most commonly used class of medications for gastrointestinal complications in cancer patients, have been reported to cause adverse renal events. However, the effect of PPIs on cisplatin-induced AKI remains unclear. Herein, the effect and mechanism of lansoprazole (LPZ), one of the most frequently prescribed PPIs, on cisplatin-induced AKI were investigated in vivo and in vitro. C57BL/6 mice received a single intraperitoneal (i.p.) injection of cisplatin (18 mg/kg) to induce AKI, and LPZ (12.5 or 25 mg/kg) was administered 2 hours prior to cisplatin administration and then once daily for another 2 days via i.p. injection. The results showed that LPZ significantly aggravated the tubular damage and further increased the elevated levels of serum creatinine and blood urea nitrogen induced by cisplatin. However, LPZ did not enhance cisplatin-induced tubular apoptosis, as evidenced by a lack of significant change in mRNA and protein expression of Bax/Bcl-2 ratio and TUNEL staining. Notably, LPZ increased the number of necrotic renal tubular cells compared to that by cisplatin treatment alone, which was further confirmed by the elevated necroptosis-associated protein expression of RIPK1, p-RIPK3 and p-MLKL. Furthermore, LPZ deteriorated cisplatin-induced inflammation, as revealed by the increased mRNA expression of pro-inflammatory factors including, NLRP3, IL-1β, TNF-α and caspase 1, as well as neutrophil infiltration. Consistently, in in vitro study, LPZ increased HK-2 cell death and enhanced inflammation, compared with cisplatin treatment alone. Collectively, our results demonstrate that LPZ aggravates cisplatin-induced AKI, and necroptosis may be involved in the exacerbation of kidney damage.
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http://dx.doi.org/10.1111/jcmm.16302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933939PMC
March 2021

The miR172/IDS1 signaling module confers salt tolerance through maintaining ROS homeostasis in cereal crops.

New Phytol 2021 05 18;230(3):1017-1033. Epub 2021 Feb 18.

State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.

Salt stress triggers the overdose accumulation of reactive oxygen species (ROS) in crop plants, leading to severe oxidative damage to living tissues. MicroRNAs (miRNAs) act as master regulators orchestrating the stress responsive regulatory networks as well as salt tolerance. However, the fundamental roles of miRNAs in modulating salt tolerance in cereal crops, especially in salt-triggered ROS scavenging remain largely unknown. Through small RNA sequencing, a salt-responsive miRNA, miR172 was identified in rice. Further, by generating the miR172-overexpression or MIR172 gene loss-of-function mutant lines, the biological significance of miR172 and its downstream signaling pathways related to salt tolerance were defined. We demonstrated that miR172 is a positive regulator of salt tolerance in both rice and wheat. More interestingly, miR172a and miR172b, but not miR172c or miR172d are involved in salt stress response, emphasizing the functional differentiation within miR172 family members. Further evidence uncovers a novel miR172/IDS1 regulatory module that functions as a crucial molecular rheostat in maintaining ROS homeostasis during salt stress, mainly through balancing the expression of a group of ROS-scavenging genes. Our findings establish a direct molecular link between miRNAs and detoxification response in cereal crops for improving salt tolerance.
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http://dx.doi.org/10.1111/nph.17211DOI Listing
May 2021

Mechanism and restoration strategy of lysosomal abnormalities induced by urinary protein overload in proximal tubule epithelial cells.

Dev Dyn 2021 Jan 6. Epub 2021 Jan 6.

Institute of Nephrology, and Key Laboratory of Prevention and Management of Chronic Kidney Disease of Zhanjiang City, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.

Background: Persistent elevated concentrations of urinary protein can destroy proximal tubule epithelial cells (PTECs) by inducing lysosomal abnormalities, thereby aggravating PTEC damage and renal fibrosis. However, the specific mechanisms of these serial biochemical events and methods for treating or preventing PTEC damage upon proteinuria need further investigation.

Results: In this study, electron microscopy and dual-labeled immunofluorescence analysis for identifying lysosome type revealed inadequate primary lysosome biogenesis and secondary lysosome accumulation in the PTECs of patients with minimal change nephrotic syndrome or membranous nephropathy who suffered from proteinuria. In vitro studies on HK-2 cells indicated that this abnormality was associated with decreased expression of transcription factor EB (TFEB). In contrast, TFEB overexpressing HK-2 cells under urinary protein overload exhibited significantly reduced accumulation of secondary lysosomes and increased proportion and quantity of primary lysosomes as indicated by dual-labeled immunofluorescence. Further, these cells could upregulate lysosomal degradation functions, as determined using Cathepsin L activity assays and flow cytometry for dye quenched-albumin.

Conclusions: These results indicate that abnormal TFEB expression is a key mechanism of lysosomal dyshomeostasis caused by protein overload in PTECs. TFEB is thus a potential therapeutic target for the treatment of urinary protein-related kidney disease.
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http://dx.doi.org/10.1002/dvdy.297DOI Listing
January 2021

Penalized-likelihood PET Image Reconstruction Using 3D Structural Convolutional Sparse Coding.

IEEE Trans Biomed Eng 2020 Dec 7;PP. Epub 2020 Dec 7.

Positron emission tomography (PET) is widely used for clinical diagnosis. As PET suffers from low resolution and high noise, numerous efforts try to incorporate anatomical priors into PET image reconstruction, especially with the development of hybrid PET/CT and PET/MRI systems. In this work, we proposed a cube-based 3D structural convolutional sparse coding (CSC) concept for penalized-likelihood PET image reconstruction, named 3D PET-CSC. The proposed 3D PET-CSC takes advantage of the convolutional operation and manages to incorporate anatomical priors without the need of registration or supervised training. As 3D PET-CSC codes the whole 3D PET image, instead of patches, it alleviates the staircase artifacts commonly presented in traditional patch-based sparse coding methods. Compared with traditional coding methods in Fourier domain, the proposed method extends the 3D CSC to a straightforward approach based on the pursuit of localized cubes. Moreover, we developed the residual-image and order-subset mechanisms to further reduce the computational cost and accelerate the convergence for the proposed 3D PET-CSC method. Experiments based on computer simulations and clinical datasets demonstrate the superiority of 3D PET-CSC compared with other reference methods.
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http://dx.doi.org/10.1109/TBME.2020.3042907DOI Listing
December 2020

CLA4 regulates leaf angle through multiple hormone signaling pathways in maize.

J Exp Bot 2021 Feb;72(5):1782-1794

College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.

Leaf angle is an important agronomic trait in cereals and shares a close relationship with crop architecture and grain yield. Although it has been previously reported that ZmCLA4 can influence leaf angle, the underlying mechanism remains unclear. In this study, we used the Gal4-LexA/UAS system and transactivation analysis to demonstrate in maize (Zea mays) that ZmCLA4 is a transcriptional repressor that regulates leaf angle. DNA affinity purification sequencing (DAP-Seq) analysis revealed that ZmCLA4 mainly binds to promoters containing the EAR motif (CACCGGAC) as well as to two other motifs (CCGARGS and CDTCNTC) to inhibit the expression of its target genes. Further analysis of ZmCLA4 target genes indicated that ZmCLA4 functions as a hub of multiple plant hormone signaling pathways: ZmCLA4 was found to directly bind to the promoters of multiple genes including ZmARF22 and ZmIAA26 in the auxin transport pathway, ZmBZR3 in the brassinosteroid signaling pathway, two ZmWRKY genes involved in abscisic acid metabolism, ZmCYP genes (ZmCYP75B1, ZmCYP93D1) related to jasmonic acid metabolism, and ZmABI3 involved in the ethylene response pathway. Overall, our work provides deep insights into the ZmCLA4 regulatory network in controlling leaf angle in maize.
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http://dx.doi.org/10.1093/jxb/eraa565DOI Listing
February 2021

FBP-Net for direct reconstruction of dynamic PET images.

Authors:
Bo Wang Huafeng Liu

Phys Med Biol 2020 Oct 13. Epub 2020 Oct 13.

the State Key Laboratory of Modern Optical Instrumentation, Zhejiang University College of Optical Science and Engineering, Hangzhou, Zhejiang, CHINA.

Dynamic positron emission tomography (PET) imaging can provide information about metabolic changes over time, used for kinetic analysis and auxiliary diagnosis. Existing deep learning-based reconstruction methods have too many trainable parameters and poor generalization, and require mass data to train the neural network. However, obtaining large amounts of medical data is expensive and time-consuming. To reduce the need for data and improve the generalization of network, we combined the filtered back-projection (FBP) algorithm with neural network, and proposed FBP-Net which could directly reconstruct PET images from sinograms instead of post-processing the rough reconstruction images obtained by traditional methods. The FBP-Net contained two parts: the filtered back-projection (FBP) part and the denoiser part. The FBP part adaptively learned the frequency filter to realize the transformation from the detector domain to the image domain, and normalized the coarse reconstruction images obtained. The denoiser part merged the information of all time frames to improve the quality of dynamic PET reconstruction images, especially the early time frames. The proposed FBP-Net was performed on simulation and real dataset, and the results were compared with the state-of-art U-net and DeepPET. The results showed that FBP-Net did not tend to overfit the training set and had a stronger generalization.
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http://dx.doi.org/10.1088/1361-6560/abc09dDOI Listing
October 2020

Micromachined Accelerometers with Sub-µg/√Hz Noise Floor: A Review.

Sensors (Basel) 2020 Jul 21;20(14). Epub 2020 Jul 21.

ESAT-MICAS, University of Leuven, 3001 Leuven, Belgium.

This paper reviews the research and development of micromachined accelerometers with a noise floor lower than 1 µg/√Hz. Firstly, the basic working principle of micromachined accelerometers is introduced. Then, different methods of reducing the noise floor of micromachined accelerometers are analyzed. Different types of micromachined accelerometers with a noise floor below 1 µg/√Hz are discussed. Such sensors can mainly be categorized into: (i) micromachined accelerometers with a low spring constant; (ii) with a large proof mass; (iii) with a high quality factor; (iv) with a low noise interface circuit; (v) with sensing schemes leading to a high scale factor. Finally, the characteristics of various micromachined accelerometers and their trends are discussed and investigated.
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http://dx.doi.org/10.3390/s20144054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411979PMC
July 2020

Deciphering the toxic effects of metals in gold mining area: Microbial community tolerance mechanism and change of antibiotic resistance genes.

Environ Res 2020 10 7;189:109869. Epub 2020 Jul 7.

School of Energy & Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, 100083, Beijing, China.

Mine tailing dumps represent significant threats to ecological environments due to the presence of toxic substances. The present work investigated the relationship among microbial activity, the community, antibiotic resistance genes (ARGs) and trace metals in soil surrounding gold mine tailings. Using microbial metabolic activity and high-throughput sequencing analysis, we found the trace metals Cd and Hg could be main factors influencing the microbial community. According to bacterial co-occurrence pattern analysis, the effects of total cadmium and total mercury on bacterial diversity are potentially mediated by influencing bacteria community in the keystone module II. Additionally, most of metal-resistant bacteria belong to Actinobacteria and Proteobacteria, and the metal tolerance suggested to be linked with various functions including replication, recombination and repair, as well as inorganic ion transport and metabolism based on PICRUSt2 analysis. We also found that metals generated by mining activity may trigger the co-selection of antibiotic resistance in the phyla Actinobacteria and Proteobacteria due to co-resistance or cross resistance. Additionally, PLS-PM analysis revealed that metals could indirectly affect ARGs by influencing bacterial diversity in gold mining areas.
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http://dx.doi.org/10.1016/j.envres.2020.109869DOI Listing
October 2020

Low-dose real-time X-ray imaging with nontoxic double perovskite scintillators.

Light Sci Appl 2020 30;9:112. Epub 2020 Jun 30.

State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang China.

X-rays are widely used in probing inside information nondestructively, enabling broad applications in the medical radiography and electronic industries. X-ray imaging based on emerging lead halide perovskite scintillators has received extensive attention recently. However, the strong self-absorption, relatively low light yield and lead toxicity of these perovskites restrict their practical applications. Here, we report a series of nontoxic double-perovskite scintillators of CsAgNaInBiCl. By controlling the content of the heavy atom Bi, the X-ray absorption coefficient, radiative emission efficiency, light yield and light decay were manipulated to maximise the scintillator performance. A light yield of up to 39,000 ± 7000 photons/MeV for CsAgNaInBiCl was obtained, which is much higher than that for the previously reported lead halide perovskite colloidal CsPbBr (21,000 photons/MeV). The large Stokes shift between the radioluminescence (RL) and absorption spectra benefiting from self-trapped excitons (STEs) led to a negligible self-absorption effect. Given the high light output and fast light decay of this scintillator, static X-ray imaging was attained under an extremely low dose of ∼1 μGy, and dynamic X-ray imaging of finger bending without a ghosting effect was demonstrated under a low-dose rate of 47.2 μGy s. After thermal treatment at 85 °C for 50 h followed by X-ray irradiation for 50 h in ambient air, the scintillator performance in terms of the RL intensity and X-ray image quality remained almost unchanged. Our results shed light on exploring highly competitive scintillators beyond the scope of lead halide perovskites, not only for avoiding toxicity but also for better performance.
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http://dx.doi.org/10.1038/s41377-020-00353-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327019PMC
June 2020

An Integrated Gold-Film Temperature Sensor for In Situ Temperature Measurement of a High-Precision MEMS Accelerometer.

Sensors (Basel) 2020 Jun 29;20(13). Epub 2020 Jun 29.

MOE Key Laboratory of Fundamental Physical Quantities Measurement & Hubei Key Laboratory of Gravitation and Quantum Physics, PGMF and School of Physics, Huazhong University of Science and Technology, Wuhan 430074, China.

Temperature sensors are one of the most important types of sensors, and are employed in many applications, including consumer electronics, automobiles and environmental monitoring. Due to the need to simultaneously measure temperature and other physical quantities, it is often desirable to integrate temperature sensors with other physical sensors, including accelerometers. In this study, we introduce an integrated gold-film resistor-type temperature sensor for in situ temperature measurement of a high-precision MEMS accelerometer. Gold was chosen as the material of the temperature sensor, for both its great resistance to oxidation and its better compatibility with our in-house capacitive accelerometer micro-fabrication process. The proposed temperature sensor was first calibrated and then evaluated. Experimental results showed the temperature measurement accuracy to be 0.08 °C; the discrepancies among the sensors were within 0.02 °C; the repeatability within seven days was 0.03 °C; the noise floor was 1 mK/√Hz@0.01 Hz and 100 μK/√Hz@0.5 Hz. The integration test with a MEMS accelerometer showed that by subtracting the temperature effect, the bias stability within 46 h for the accelerometer could be improved from 2.15 μg to 640 ng. This demonstrates the capability of measuring temperature in situ with the potential to eliminate the temperature effects of the MEMS accelerometer through system-level compensation.
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http://dx.doi.org/10.3390/s20133652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374507PMC
June 2020

Rapid image deconvolution and multiview fusion for optical microscopy.

Nat Biotechnol 2020 11 29;38(11):1337-1346. Epub 2020 Jun 29.

Laboratory of High-Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.

The contrast and resolution of images obtained with optical microscopes can be improved by deconvolution and computational fusion of multiple views of the same sample, but these methods are computationally expensive for large datasets. Here we describe theoretical and practical advances in algorithm and software design that result in image processing times that are tenfold to several thousand fold faster than with previous methods. First, we show that an 'unmatched back projector' accelerates deconvolution relative to the classic Richardson-Lucy algorithm by at least tenfold. Second, three-dimensional image-based registration with a graphics processing unit enhances processing speed 10- to 100-fold over CPU processing. Third, deep learning can provide further acceleration, particularly for deconvolution with spatially varying point spread functions. We illustrate our methods from the subcellular to millimeter spatial scale on diverse samples, including single cells, embryos and cleared tissue. Finally, we show performance enhancement on recently developed microscopes that have improved spatial resolution, including dual-view cleared-tissue light-sheet microscopes and reflective lattice light-sheet microscopes.
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http://dx.doi.org/10.1038/s41587-020-0560-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642198PMC
November 2020

Functions and regulatory framework of ZmNST3 in maize under lodging and drought stress.

Plant Cell Environ 2020 09 23;43(9):2272-2286. Epub 2020 Jul 23.

College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, China.

The growth and development of maize are negatively affected by various abiotic stresses including drought, high salinity, extreme temperature, and strong wind. Therefore, it is important to understand the molecular mechanisms underlying abiotic stress resistance in maize. In the present work, we identified that a novel NAC transcriptional factor, ZmNST3, enhances maize lodging resistance and drought stress tolerance. ChIP-Seq and expression of target genes analysis showed that ZmNST3 could directly regulate the expression of genes related to cell wall biosynthesis which could subsequently enhance lodging resistance. Furthermore, we also demonstrated that ZmNST3 affected the expression of genes related to the synthesis of antioxidant enzyme secondary metabolites that could enhance drought resistance. More importantly, we are the first to report that ZmNST3 directly binds to the promoters of CESA5 and Dynamin-Related Proteins2A (DRP2A) and activates the expression of genes related to secondary cell wall cellulose biosynthesis. Additionally, we revealed that ZmNST3 directly binds to the promoters of GST/GlnRS and activates genes which could enhance the production of antioxidant enzymes in vivo. Overall, our work contributes to a comprehensive understanding of the regulatory network of ZmNST3 in regulating maize lodging and drought stress resistance.
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http://dx.doi.org/10.1111/pce.13829DOI Listing
September 2020

Emerging role of transcription factor EB in mitochondrial quality control.

Biomed Pharmacother 2020 Aug 22;128:110272. Epub 2020 May 22.

Key Laboratory of Prevention and Management of Chronic Kidney Disease of Zhanjiang City, Institute of Nephrology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China. Electronic address:

Mitochondria are energy producers that play a vital role in cell survival. Mitochondrial dysfunction is involved in many diseases, including metabolic syndrome, neurodegenerative disorders, cardiomyopathies, cancer, obesity, and diabetic kidney disease, and challenges still remain in terms of treatments for these diseases. Mitochondrial quality control (MQC), which is defined as the maintenance of the quantity, morphology, and function of mitochondria, plays a pivotal role in maintaining cellular metabolic homeostasis and cell survival. Recently, growing evidence suggests that the transcription factor EB (TFEB) plays a pivotal role in MQC. Here, we systemically investigate the potential role and mechanisms of TFEB in MQC, which include the activation of mitophagy, regulation of mitochondrial biogenesis, reactive oxygen species (ROS) clearance, and the balance of mitochondria fission-fusion cycle. Importantly, we further discuss the therapeutic measures and effects aimed at TFEB on mitochondrial dysfunction-related diseases. Taken together, targeting TFEB to regulate MQC may represent an appealing therapeutic strategy for mitochondrial dysfunction related-diseases.
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http://dx.doi.org/10.1016/j.biopha.2020.110272DOI Listing
August 2020

Regulation of TFEB activity and its potential as a therapeutic target against kidney diseases.

Cell Death Discov 2020 1;6:32. Epub 2020 May 1.

Institute of Nephrology, and Key Laboratory of Prevention and Management of Chronic Kidney Disease of Zhanjiang City, Affiliated Hospital of Guangdong Medical University, 524001 Zhanjiang, Guangdong China.

The transcription factor EB (TFEB) regulates the expression of target genes bearing the Coordinated Lysosomal Expression and Regulation (CLEAR) motif, thereby modulating autophagy and lysosomal biogenesis. Furthermore, TFEB can bind to the promoter of autophagy-associated genes and induce the formation of autophagosomes, autophagosome-lysosome fusion, and lysosomal cargo degradation. An increasing number of studies have shown that TFEB stimulates the intracellular clearance of pathogenic factors by enhancing autophagy and lysosomal function in multiple kidney diseases, such as cystinosis, acute kidney injury, and diabetic nephropathy. Taken together, this highlights the importance of developing novel therapeutic strategies against kidney diseases based on TFEB regulation. In this review, we present an overview of the current data on TFEB and its implication in kidney disease.
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http://dx.doi.org/10.1038/s41420-020-0265-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195473PMC
May 2020

Historical residues of organochlorine pesticides (OCPs) and polycyclic aromatic hydrocarbons (PAHs) in a flood sediment profile from the Longwang Cave in Yichang, China.

Ecotoxicol Environ Saf 2020 Jun 7;196:110542. Epub 2020 Apr 7.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430078, China.

Historical residual of persistent organic pollutants (POPs) in flood sediment from a karst cave were investigated. Fifteen vertical sediment samples were collected from a 6 m-deep flood sediment profile in the Longwang Cave, and the concentrations of organochlorine pesticides (OCPs), polycyclic aromatic hydrocarbons (PAHs) and particle size distribution in the sediments were analysed. The concentrations of OCPs and PAHs varied from 0.85 ng g to 63.1 ng g (mean 8.11 ng g) and 5.05 ng g to 82.6 ng g (mean 13.9 ng g), respectively; major PAHs in the profile were 2- and 3-ringed PAHs and a few were 5- or 6- ringed PAHs, which indicated less influence from industry but a high impact from the local combustion of coal and biomass; HCHs and DDTs in the profile were historically residual in this region, and HCHs mainly originated from the application of Lindane, while DDTs originated from the application of dicofol and technical DDTs; no significant correlation between the concentrations of OCPs and PAHs and the sedimental particle size in the sediments was found.
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http://dx.doi.org/10.1016/j.ecoenv.2020.110542DOI Listing
June 2020

Comparative analysis of electrocardiographic imaging and ECG in predicting the origin of outflow tract ventricular arrhythmias.

J Int Med Res 2020 Mar;48(3):300060520913132

Department of Cardiology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.

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http://dx.doi.org/10.1177/0300060520913132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132561PMC
March 2020

Interactions between dicyandiamide and periphytic biofilms in paddy soils and subsequent effects on nitrogen cycling.

Sci Total Environ 2020 May 19;718:137417. Epub 2020 Feb 19.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address:

Dicyandiamide (DCD) is commonly used as nitrification inhibitors which has the potential to reduce nitrogen loss from paddy soils. In paddy systems, periphytic biofilms are commonly presented at the soil/water interface and show significant effects on nutrient cycling. However, the interaction between DCD and periphytic biofilms in paddy and subsequent effects on nitrogen cycling is unclear. In this work, microcosm experiments were carried out to study the interaction between the periphytic biofilms and DCD and the potential influence on nitrogen cycling from in paddy. Results showed that DCD affected the development of periphytic biofilms, while the presence of periphytic biofilms accelerated DCD degradation. Results also showed DCD application reduced nitrification potential mainly by inhibiting ammonia-oxidizing bacteria (AOB). Higher DCD dosage increased NH volatilization loss. However, presence of periphytic biofilm reduced the NH volatilization loss but increased denitrification. Our work contributes to a better understanding on the nitrogen cycling processes in paddy, and provides useful information for the improvement of nitrogen utilization efficiency and the control of non-point source pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2020.137417DOI Listing
May 2020

Temperature Gradient Method for Alleviating Bonding-Induced Warpage in a High-Precision Capacitive MEMS Accelerometer.

Sensors (Basel) 2020 Feb 21;20(4). Epub 2020 Feb 21.

MOE Key Laboratory of Fundamental Physical Quantities Measurement, School of Physic, Huazhong University of Science and Technology, Wuhan 430074, China.

Capacitive MEMS accelerometers with area-variable periodic-electrode displacementtransducers found wide applications in disaster monitoring, resource exploration and inertialnavigation. The bonding-induced warpage, due to the difference in the coefficients of thermalexpansion of the bonded slices, has a negative influence on the precise control of the interelectrodespacing that is essential to the sensitivity of accelerometers. In this work, we propose the theory,simulation and experiment of a method that can alleviate both the stress and the warpage byapplying different bonding temperature on the bonded slices. A quasi-zero warpage is achievedexperimentally, proving the feasibility of the method. As a benefit of the flat surface, the spacing ofthe capacitive displacement transducer can be precisely controlled, improving the self-noise of theaccelerometer to 6 ng/√Hz @0.07 Hz, which is about two times lower than that of the accelerometerusing a uniform-temperature bonding process.
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http://dx.doi.org/10.3390/s20041186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071001PMC
February 2020

Non-invasive reconstruction of dynamic myocardial transmembrane potential with graph-based total variation constraints.

Healthc Technol Lett 2019 Dec 26;6(6):181-186. Epub 2019 Nov 26.

State Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.

Non-invasive reconstruction of electrophysiological activity in the heart is of great significance for clinical disease prevention and surgical treatment. The distribution of transmembrane potential (TMP) in three-dimensional myocardium can help us diagnose heart diseases such as myocardial ischemia and ectopic pacing. However, the problem of solving TMP is ill-posed, and appropriate constraints need to be added. The existing state-of-art method total variation minimisation only takes advantage of the local similarity in space, which has the problem of over-smoothing, and fails to take into account the relationship among frames in the dynamic TMP sequence. In this work, the authors introduce a novel regularisation method called graph-based total variation to make up for the above shortcomings. The graph structure takes the TMP value of a time sequence on each heart node as the criterion to establish the similarity relationship among the heart. Two sets of phantom experiments were set to verify the superiority of the proposed method over the traditional constraints: infarct scar reconstruction and activation wavefront reconstruction. In addition, experiments with ten real premature ventricular contractions patient data were used to demonstrate the accuracy of the authors' method in clinical applications.
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http://dx.doi.org/10.1049/htl.2019.0065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945684PMC
December 2019

ZmIBH1-1 regulates plant architecture in maize.

J Exp Bot 2020 05;71(10):2943-2955

College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.

Leaf angle (LA) is a critical agronomic trait in maize, with more upright leaves allowing higher planting density, leading to more efficient light capture and higher yields. A few genes responsible for variation in LA have been identified by map-based cloning. In this study, we cloned maize ZmIBH1-1, which encodes a bHLH transcription factor with both a basic binding region and a helix-loop-helix domain, and the results of qRT-PCR showed that it is a negative regulator of LA. Histological analysis indicated that changes in LA were mainly caused by differential cell wall lignification and cell elongation in the ligular region. To determine the regulatory framework of ZmIBH1-1, we conducted RNA-seq and DNA affinity purification (DAP)-seq analyses. The combined results revealed 59 ZmIBH1-1-modulated target genes with annotations, and they were mainly related to the cell wall, cell development, and hormones. Based on the data, we propose a regulatory model for the control of plant architecture by ZmIBH1-1 in maize.
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http://dx.doi.org/10.1093/jxb/eraa052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260713PMC
May 2020

Imaging mitochondrial complex I activation during a vibrotactile stimulation: A PET study using [F]BCPP-EF in the conscious monkey brain.

J Cereb Blood Flow Metab 2020 12 16;40(12):2521-2532. Epub 2020 Jan 16.

State Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou, China.

In order to evaluate the capability of 2--butyl-4-chloro-5-{6-[2-(2-[F]fluoroethoxy)-ethoxy]-pyridin-3-ylmethoxy}-2H-pyridazin-3-one ([F]BCPP-EF), a novel positron emission tomography (PET) probe for mitochondrial complex I (MC-I) activity, to assess neuronal activation, an activation PET study was conducted in the conscious monkey brain with a continuous unilateral vibrotactile stimulation. PET scans with [O]HO, [F]BCPP-EF, or 2-deoxy-2-[F]fluoroglucose ([F]FDG) were conducted under: (1) resting conditions; (2) a continuous vibration stimulation; (3) a continuous vibration stimulation after 15-min pre-vibration; and (4) a continuous vibration stimulation after 30-min pre-vibration. The contralateral/ipsilateral ratio (CIR) in the somatosensory cortex showed significant increases in the uptake of [O]HO, [F]BCPP-EF, and [F]FDG with the vibration stimulation. The longer pre-vibration duration induced significantly lower CIR in regional cerebral blood flow (rCBF) measured using [O]HO, whereas it did not affect the CIR in [F]BCPP-EF or the regional cerebral metabolic rate of glucose (rCMRglc) measured using [F]FDG 30-60 min after the injection. These results suggest that the [F]BCPP-EF response in the later phase of scans was not influenced by the increase in rCBF, indicating the capability of [F]BCPP-EF to detect acute changes in MC-I activity induced by neuronal activation. However, the metabolic shift from glycolysis to oxidation was not observed under the stimulation used here.
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http://dx.doi.org/10.1177/0271678X19900034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820687PMC
December 2020

Timing calibration for PET based on low rank constraint.

Annu Int Conf IEEE Eng Med Biol Soc 2019 Jul;2019:4836-4839

Timing calibration in PET is of great importance for improving time resolution. In this paper, we present a new computational strategy for the framework based upon the low rank principles, solved through the ADMM. The linear equations of time delay is constructed using a point source or a phantom. The low rank property captures spatiotemporal correlation of noise-corrupted measurement. Experiments with Monte Carlo simulation and real data have been conducted with very promising results, as validated through comparison to some existing methods.
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http://dx.doi.org/10.1109/EMBC.2019.8857624DOI Listing
July 2019

3D Tensor Based Nonlocal Low Rank Approximation in Dynamic PET Reconstruction.

Sensors (Basel) 2019 Dec 1;19(23). Epub 2019 Dec 1.

State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, China.

Reconstructing images from multi-view projections is a crucial task both in the computer vision community and in the medical imaging community, and dynamic positron emission tomography (PET) is no exception. Unfortunately, image quality is inevitably degraded by the limitations of photon emissions and the trade-off between temporal and spatial resolution. In this paper, we develop a novel tensor based nonlocal low-rank framework for dynamic PET reconstruction. Spatial structures are effectively enhanced not only by nonlocal and sparse features, but momentarily by tensor-formed low-rank approximations in the temporal realm. Moreover, the total variation is well regularized as a complementation for denoising. These regularizations are efficiently combined into a Poisson PET model and jointly solved by distributed optimization. The experiments demonstrated in this paper validate the excellent performance of the proposed method in dynamic PET.
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http://dx.doi.org/10.3390/s19235299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928938PMC
December 2019

Modular segregation of task-dependent brain networks contributes to the development of executive function in children.

Neuroimage 2020 02 5;206:116334. Epub 2019 Nov 5.

State Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou, 310027, China. Electronic address:

Executive function (EF) refers as to a set of high-level cognitive abilities that are critical to many aspects of daily life. Despite its importance in human daily life, the neural networks responsible for the development of EF in childhood are not well understood. The present study thus aimed to examine the development of task-dependent brain network organization and its relationship to age-related improvements in EF. To address this issue, we recruited eighty-eight Chinese children ranging in age from 7 to 12 years old, and collected their functional magnetic resonance imaging (fMRI) data when they performed an EF task. By utilizing graph theory, we found that the task-dependent brain network modules became increasingly segregated with age. Specifically, the intra-module connections within the default-mode network (DMN), frontal-parietal network (FPN) and sensorimotor network (SMN) increased significantly with age. In contrast, the inter-module connections of the visual network to both the FPN/SMN decreased significantly with age. Most importantly, modular segregation of the FPN significantly mediated the relationship between age and EF performance. These findings add to our growing understanding of how development changes in task-dependent brain network organization support vast behavioral improvements in EF observed during childhood.
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http://dx.doi.org/10.1016/j.neuroimage.2019.116334DOI Listing
February 2020

A high-sensitivity MEMS gravimeter with a large dynamic range.

Microsyst Nanoeng 2019 7;5:45. Epub 2019 Oct 7.

1MOE Key Laboratory of Fundamental Physical Quantities Measurement & Hubei Key Laboratory of Gravitation and Quantum Physics, PGMF and School of Physics, Huazhong University of Science and Technology, 430074 Wuhan, PR China.

Precise measurement of variations in the local gravitational acceleration is valuable for natural hazard forecasting, prospecting, and geophysical studies. Common issues of the present gravimetry technologies include their high cost, high mass, and large volume, which can potentially be solved by micro-electromechanical-system (MEMS) technology. However, the reported MEMS gravimeter does not have a high sensitivity and a large dynamic range comparable with those of the present commercial gravimeters, lowering its practicability and ruling out worldwide deployment. In this paper, we introduce a more practical MEMS gravimeter that has a higher sensitivity of 8 μGal/√Hz and a larger dynamic range of 8000 mGal by using an advanced suspension design and a customized optical displacement transducer. The proposed MEMS gravimeter has performed the co-site earth tides measurement with a commercial superconducting gravimeter GWR iGrav with the results showing a correlation coefficient of 0.91.
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http://dx.doi.org/10.1038/s41378-019-0089-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799805PMC
October 2019

ZmILI1 regulates leaf angle by directly affecting liguleless1 expression in maize.

Plant Biotechnol J 2020 04 1;18(4):881-883. Epub 2019 Oct 1.

College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key, Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, China.

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http://dx.doi.org/10.1111/pbi.13255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061864PMC
April 2020

PET image denoising using unsupervised deep learning.

Eur J Nucl Med Mol Imaging 2019 Dec 29;46(13):2780-2789. Epub 2019 Aug 29.

Center for Advanced Medical Computing and Analysis, Massachusetts General Hospital, 55 Fruit St, White 427, Boston, MA, 02114, USA.

Purpose: Image quality of positron emission tomography (PET) is limited by various physical degradation factors. Our study aims to perform PET image denoising by utilizing prior information from the same patient. The proposed method is based on unsupervised deep learning, where no training pairs are needed.

Methods: In this method, the prior high-quality image from the patient was employed as the network input and the noisy PET image itself was treated as the training label. Constrained by the network structure and the prior image input, the network was trained to learn the intrinsic structure information from the noisy image and output a restored PET image. To validate the performance of the proposed method, a computer simulation study based on the BrainWeb phantom was first performed. A Ga-PRGD2 PET/CT dataset containing 10 patients and a F-FDG PET/MR dataset containing 30 patients were later on used for clinical data evaluation. The Gaussian, non-local mean (NLM) using CT/MR image as priors, BM4D, and Deep Decoder methods were included as reference methods. The contrast-to-noise ratio (CNR) improvements were used to rank different methods based on Wilcoxon signed-rank test.

Results: For the simulation study, contrast recovery coefficient (CRC) vs. standard deviation (STD) curves showed that the proposed method achieved the best performance regarding the bias-variance tradeoff. For the clinical PET/CT dataset, the proposed method achieved the highest CNR improvement ratio (53.35% ± 21.78%), compared with the Gaussian (12.64% ± 6.15%, P = 0.002), NLM guided by CT (24.35% ± 16.30%, P = 0.002), BM4D (38.31% ± 20.26%, P = 0.002), and Deep Decoder (41.67% ± 22.28%, P = 0.002) methods. For the clinical PET/MR dataset, the CNR improvement ratio of the proposed method achieved 46.80% ± 25.23%, higher than the Gaussian (18.16% ± 10.02%, P < 0.0001), NLM guided by MR (25.36% ± 19.48%, P < 0.0001), BM4D (37.02% ± 21.38%, P < 0.0001), and Deep Decoder (30.03% ± 20.64%, P < 0.0001) methods. Restored images for all the datasets demonstrate that the proposed method can effectively smooth out the noise while recovering image details.

Conclusion: The proposed unsupervised deep learning framework provides excellent image restoration effects, outperforming the Gaussian, NLM methods, BM4D, and Deep Decoder methods.
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http://dx.doi.org/10.1007/s00259-019-04468-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814987PMC
December 2019

The Effect of Antioxidant Vitamins on Patients With Diabetes and Albuminuria: A Meta-Analysis of Randomized Controlled Trials.

J Ren Nutr 2020 03 26;30(2):101-110. Epub 2019 Aug 26.

Key Laboratory of Prevention and Management of Chronic Kidney Disease of Zhanjiang City, Affiliated Hospital of Guangdong Medical University, Zhanjiang, People's Republic of China. Electronic address:

Objective: This study aimed to investigate the effect of antioxidant vitamins, including vitamins E and C, on patients with diabetes and albuminuria by conducting a meta-analysis of randomized controlled trials.

Design: The PubMed, Embase, CENTRAL (the Cochrane Central Register of Controlled Trials at the Cochrane Library), Web of Science, OVID, and www.clinicaltrials.gov (latest search: December 10, 2018) databases were searched. This study was limited to randomized controlled trials. Patients with diabetes and albuminuria were included regardless of diabetic type, and patients must have received treatment with vitamins C or E.

Results: Ten studies, representing 445 participants, were identified for analysis. Antioxidant vitamins had significant effects on serum creatinine levels (mean difference = -0.11 mg/dL, 95% confidence interval -0.19 to -0.03, P = .007) and systolic pressure (mean difference = -6.02 mm Hg, 95% confidence interval -9.65 to -2.40, P = .001) with low heterogeneity. Antioxidant vitamins had no effect on albuminuria or proteinuria, diastolic blood pressure, glucose, or lipid metabolism.

Conclusion: This meta-analysis indicated that antioxidant vitamins can benefit kidney function and systolic blood pressure in patients with diabetes and albuminuria. Further studies with larger sample sizes and longer follow-up are needed to completely understand the effect of antioxidant vitamins in these patients.
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http://dx.doi.org/10.1053/j.jrn.2019.06.011DOI Listing
March 2020