Publications by authors named "Huabin Chen"

26 Publications

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Metabolome Analysis Revealed the Mechanism of Exogenous Glutathione to Alleviate Cadmium Stress in Maize ( L.) Seedlings.

Plants (Basel) 2021 Jan 6;10(1). Epub 2021 Jan 6.

Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Yu-Hang-Tang Road No 866, Hangzhou 310058, China.

Cadmium (Cd) is one of the major heavy metal pollutants in the environment and imposes severe limitations on crop growth and production. Glutathione (GSH) plays an important role in plant Cd tolerance which is able to scavenge stresses-induced reactive oxygen species (ROS) and is involved in the biosynthesis of phytochelatins (PCs). Our previous study revealed that Cd stress affects maize growth, and the GSH treatment could relieve Cd stress in maize seedlings. In this study, we attempted to characterize the metabolomics changes in maize leaves and roots under Cd stress and exogenous GSH conditions. We identified 145 and 133 metabolites in the leaves and roots, respectively. Cd stress decreased the tricarboxylic acid cycle (TCA cycle) metabolism and increased the amino acid contents in the leaves, while it decreased the amino acid contents, increased the TCA cycle metabolism, the sugar contents, and shikimic acid metabolism in the roots. On the other hand, exogenous GSH increased the GSH content, changed the production of metabolites related to antioxidant systems (such as ascorbic acid-related metabolites and flavonoid-related metabolites), and alleviated lipid peroxidation, thereby alleviating the toxic effect of Cd stress on maize. These findings support the idea that GSH alleviates Cd-induced stress in maize and may help to elucidate the mechanism governing Cd-induced stress and the GSH-driven alleviation effect.
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http://dx.doi.org/10.3390/plants10010105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825527PMC
January 2021

Clinical outcomes of acute pulmonary embolectomy as the first-line treatment for massive and submassive pulmonary embolism: a single-centre study in China.

J Cardiothorac Surg 2020 Oct 21;15(1):321. Epub 2020 Oct 21.

Fujian Medical University, Fuzhou, 350001, Fujian, China.

Background: Acute pulmonary embolism (PE) is one of the most critical cardiovascular diseases. PE treatment ranges from anticoagulation, and systemic thrombolysis to surgical embolectomy and catheter embolectomy. Surgical pulmonary embolectmy (SPE) indications and outcomes are still controversial. Although there have been more favourable SPE reports over the past decades, SPE has not yet been considered broadly as an initial PE therapy and is still considered as a reserve or rescue treatment for acute massive PE when systemic thrombolysis fails. This study aimed to evaluate the early and midterm outcomes of SPE, which was a first-line therapy for acute central major PE in one Chinese single centre.

Methods: A retrospective review of patients who underwent SPE for acute PE was conducted.Patients with chronic thrombus or who underwent thromboendarterectomy were excluded. SPE risk factors for morbidity and mortality were reviewed, and echocardiographic examination were conducted for follow-up studies to access right ventricular function.

Results: Overall, 41 patients were included; 17 (41.5%) had submassive PE, and 24 (58.5%) had massive PE. Mean cardiopulmonary bypass time was 103.2 ± 48.9 min, and 10 patients (24.4%) underwent procedures without aortic cross-clamping. Ventilatory support time was 78 h (range, 40-336 h), intensive care unit stay was 7 days (range, 3-13 days), and hospital stay was 16 days (range, 12-23 days). Operative mortalities occurred in 3 massive PE patients, and no mortality occurred in submassive PE patients. The overall SPE mortality rate was 7.31% (3/41). If two systemic thrombolysis cases were excluded, SPE mortality was low (2.56%,1/39), evenlthough there were 2 cases of cardiac arrest preoperatively. Patients' right ventricle function improved postoperatively in follow-ups.There were no deaths related to recurrent PE and chronic pulmonary hypertension in follow-ups, though 3 patients died of cerebral intracranial bleeding, gastric cancer,and brain cancer at 1 year, 3 years, and 8 years postoperatively, respectively.

Conclusions: SPE presented with a low mortality rate when rendered as a first-line treatment in selected massive and submassive acute PE patients. Favorable outcomes of right ventricle function were also observed in the follow-ups. SPE should play the same role as ST in algorithmic acute PE treatment.
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http://dx.doi.org/10.1186/s13019-020-01364-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576708PMC
October 2020

Treadmill running initiation times and bone-tendon interface repair in a murine rotator cuff repair model.

J Orthop Res 2020 Sep 16. Epub 2020 Sep 16.

Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, People's Republic of China.

Postoperative exercise has been demonstrated to be beneficial for bone-tendon interface (BTI) healing, yet the debate regarding the optimal time to initiate exercise after tendon enthesis repair is ongoing. This study aimed to evaluate the initiation times for exercise after enthesis repair. A total of 192 C57BL/6 mice underwent acute supraspinatus tendon injury repair. The animals were then randomly assigned to four groups: free cage activity after repair (control group); treadmill running started on postoperative day 2 (2-day delayed group); treadmill running started on postoperative day 7 (7-day delayed group), and treadmill running started on postoperative day 14 (14-day delayed group). Mice were euthanized at 4 and 8 weeks postoperatively, and histological, biomechanical, and bone morphometric tests were performed. Higher failure loads and bone volume fractions were found for the 7-day delayed group and the 14-day delayed group at 4 weeks postoperatively. The 7-day delayed group had better biomechanical properties and higher bone volume fractions than the 2-day delayed group at 4 weeks postoperatively. Histologically, the 7-day delayed group exhibited lower modified tendon-to-bone maturity scores than the control group and the 2-day delayed group at 4 and 8 weeks postoperatively. Quantitative reverse-transcription polymerase chain reaction results showed that the 7-day delayed group had higher expressions of chondrogenic- and osteogenic-related genes. Statement of clinical significance: Postoperative treadmill running initiated on postoperative day 7 had a more prominent effect on BTI healing than other treatment regimens in this study and could accelerate BTI healing and rotator cuff repair.
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http://dx.doi.org/10.1002/jor.24863DOI Listing
September 2020

Periosteum progenitors could stimulate bone regeneration in aged murine bone defect model.

J Cell Mol Med 2020 10 15;24(20):12199-12210. Epub 2020 Sep 15.

Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.

Periosteal stem cells are critical for bone regeneration, while the numbers will decrease with age. This study focused on whether Prx1 cell, a kind of periosteal stem cell, could stimulate bone regeneration in aged mice. Four weeks and 12 months old Prx1CreER-GFP; Rosa26 mice were used to reveal the degree of Prx1 cells participating in the femoral fracture healing procedure. One week, 8 weeks, 12 and 24 months old Prx1CreER-GFP mice were used to analyse the real-time distribution of Prx1 cells. Twelve months old C57BL/6 male mice (n = 96) were used to create the bone defect model and, respectively, received hydrogel, hydrogel with Prx1 mesenchymal stem cells and hydrogel with Prx1 cells. H&E staining, Synchrotron radiation-microcomputed tomography and mechanical test were used to analyse the healing results. The results showed that tdTomato cells were involved in bone regeneration, especially in young mice. At the same time, GFP cells decreased significantly with age. The Prx1 cells group could significantly improve bone regeneration in the murine bone defect model via directly differentiating into osteoblasts and had better osteogenic differentiation ability than Prx1 mesenchymal stem cells. Our finding revealed that the quantity of Prx1 cells might account for decreased bone regeneration ability in aged mice, and transplantation of Prx1 cells could improve bone regeneration at the bone defect site.
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http://dx.doi.org/10.1111/jcmm.15891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579685PMC
October 2020

Comparison of bone surface and trough fixation on bone-tendon healing in a rabbit patella-patellar tendon injury model.

J Orthop Translat 2020 Mar 18;21:49-56. Epub 2020 Jan 18.

Department of Sports Medicine & Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.

Background: Many orthopedic surgical procedures involve reattachment between tendon and bone. Whether bone-tendon healing is better facilitated by tendon fixation on a bone surface or within a tunnel is unknown. The purpose of this study was to comparatively evaluate the effects of bone surface versus bone trough fixation on bone-tendon healing in a rabbit patella-patellar tendon (PPT) injury model.

Methods: The rabbits underwent partial patellectomy with patellar-tendon fixation on the osteotomy surface (bone surface fixation, BSF group) (n = 28) or within a bone trough (bone trough fixation, BTF group) (n = 28). The PPT interface was evaluated by macroscopic observation, micro-computed tomography scanning, histological analysis, and biomechanical testing at postoperative week 8 or week 16.

Results: Macroscopically, no signs of infection or osteoarthritis were observed, and the regenerated tissue bridging the residual patella and patellar tendon showed no obvious difference between the two groups. There were significantly higher bone mineral density ​and trabecular thickness ​in BSF group compared with BTF group at week 8 ( ​< ​0.05 for both). However, the bone volume fraction (BVF), bone mineral density and trabecular thickness in BSF group were significantly lower than those in BTF group ( ​< ​0.05 for all) at week 16. Histological analysis demonstrated that new bone was formed at the proximal patella and reattached to the residual patellar tendon through a regenerated fibrocartilage-like tissue in both groups. There was more formation and better remodelling of fibrocartilage-like tissue in BTF group than BSF group at week 8 and week 16 ( ​< ​0.05 for both). Biomechanical testing revealed that there was higher failure load and stiffness at the PPT interface in BTF group than BSF group at week 16 ( ​< ​0.05 for both).

Conclusions: These results suggested that raptured tendon fixation in a bone trough resulted in superior bone-tendon healing in comparison with tendon fixation on bone surface in a rabbit PPT injury model.

The Translational Potential Of This Article: Although the structural and functional difference of knee joint between human and rabbit limit the results to be directly used in clinical, our research does offer a valuable reference for the improvement of reattachment between bone and tendon.
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http://dx.doi.org/10.1016/j.jot.2019.12.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029051PMC
March 2020

Autologous Freeze-Dried, Platelet-Rich Plasma Carrying Icariin Enhances Bone-Tendon Healing in a Rabbit Model.

Am J Sports Med 2019 07 31;47(8):1964-1974. Epub 2019 May 31.

Department of Sports Medicine & Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.

Background: Tendon-bone interface (TBI) injuries are common in sports activities. Owing to the limited regenerative ability of the TBI, its functional healing remains a difficulty in clinical practice. Icariin (ICA) provides strong stimulation for osteogenesis. Platelet-rich plasma (PRP) can be used as a carrier for bioactive molecules, although its ability to provide sustained release for such molecules needs improvement.

Hypothesis: Freeze-dried PRP (FD-PRP) as a carrier for ICA can provide sustained release of ICA into the tendon-bone (T-B) healing site, thus accelerating T-B healing.

Study Design: Controlled laboratory study.

Methods: A total of 84 New Zealand rabbits with partial patellectomy in the hindlimb were randomly allocated into 3 different treatments: ICA incorporated with FD-PRP (ICA/FD-PRP), FD-PRP alone (FD-PRP), or saline control (CTL). The rabbit patella-patellar tendon (PP) interfaces were postoperatively harvested at postoperative week 8 or 16 for gross, radiological, histological, and mechanical evaluations.

Results: Our results showed that FD-PRP can act as a carrier for sustained release of ICA into the T-B healing site. Macroscopically, no signs of infection or osteoarthritis were shown in the regenerated PP interfaces, and the area of cartilaginous metaplasia in the FD-PRP and ICA/FD-PRP groups at postoperative week 16 was significantly larger than that of the CTL group ( < .05 for all). Radiologically, micro-computed tomography showed that new bone which formed at the healing site in the ICA/FD-PRP group was significantly increased, remodeled, and mineralized in comparison with the CTL group ( < .05 for all). Histologically, the ICA/FD-PRP group exhibited a significant native PP interface, as shown by the enlargement and remodeling of new bone, well-organized collagen fibers, and robust production of proteoglycans in the regenerated fibrocartilage. The mechanical strength of the regenerated PP interface was significantly improved in the ICA/FD-PRP group. Significantly higher failure load and stiffness were shown in the ICA/FD-PRP group compared with the CTL and FD-PRP groups, respectively ( < .05 for all).

Conclusion: FD-PRP is a suitable sustained-release carrier for ICA, and ICA/FD-PRP can provide sustained release of ICA into the T-B healing site, thus effectively accelerating T-B healing.

Clinical Relevance: Findings of this study demonstrate the feasibility of using FD-PRP as a carrier for ICA to improve T-B healing and provide a foundation for future clinical application.
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http://dx.doi.org/10.1177/0363546519849657DOI Listing
July 2019

Deriving a Ultraviolet-Visible-Near-Infrared-Active Photocatalyst from Calcination of an Mg/Zn/Al/Er-Hydrotalcite-Like Compound.

J Nanosci Nanotechnol 2019 Nov;19(11):7169-7177

Key Laboratory of Pulp & Paper Science and Technology (Ministry of Education) Qilu University of Technology, Jinan, Shandong 250353, China.

Developing full-spectrum photocatalysts that harvests solar light from ultraviolet to near-infrared light has aroused great interest in photodegradation of organic pollutants, due to the imminent energy crisis and growing pollution issues. Herein, we report an excellent full-spectrum photocatalyst derived from calcination of an Mg/Zn/Al/Er-hydrotalcite-like compound. The photocatalyst is a stable multi-phase oxide consisting of various syntrophic Er-doped metal oxides with different particle sizes and morphology. Its ultraviolet (UV) photocatalytic activity is maximized by increasing the fraction of Zn and sustaining the pure hydrotalcite-like phase with an appropriate fraction of Mg in preparing the Mg/Zn/Al/Er-hydrotalcite-like precursor. The visible and NIR photocatalytic activities are triggered by an indirect excitation involving an up-conversion process. The major active species of the photocatalyst in the photodegradation of methyl orange are superoxide anions and photogenerated holes. Nevertheless, hydroxyl radicals also play a moderate role in the photodegradation process. This work finds a new way to prepare full-spectrum photocatalysts with tunable chemical compositions via an environmentally friendly hydrotalcite-like precursor.
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http://dx.doi.org/10.1166/jnn.2019.16614DOI Listing
November 2019

Effect of Low-Intensity Pulsed Ultrasound After Autologous Adipose-Derived Stromal Cell Transplantation for Bone-Tendon Healing in a Rabbit Model.

Am J Sports Med 2019 03;47(4):942-953

Department of Sports Medicine & Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.

Background: Low-intensity pulsed ultrasound (LIPUS), as a safe biophysiotherapy, can enhance bone-tendon (B-T) healing in vivo and induce osteogenic or chondrogenic differentiation of mesenchymal stromal cells in vitro. This study aimed to determine whether LIPUS can improve the efficacy of transplanted mesenchymal stromal cells on B-T healing.

Hypothesis: LIPUS can induce lineage-specific differentiation of transplanted adipose-derived stromal cells (ASCs) at the B-T healing site, thus resulting in superior healing quality when compared with LIPUS or ASCs alone.

Study Design: Controlled laboratory study.

Methods: A total of 112 mature rabbits with partial patellectomy in the hindlimb were randomly assigned into mock sonication without ASCs (control), ultrasonication without ASCs (LIPUS), mock sonication with ASCs (ASCs), and ultrasonication with ASCs (LIPUS + ASCs). The treatment time of the mock sonication or ultrasonication was 20 minutes per day. Autologous ASCs were transplanted to the healing site by fibrin glue during the operation, and LIPUS was delivered daily starting at postoperative day 3 until euthanasia. The patella-patellar tendon junctions were postoperatively harvested at 8 and 16 weeks for radiological, histological, and mechanical evaluations. Additionally, 9 animals were used for ASC tracking with mCherry protein.

Results: Radiologically, there was more new bone formation and remodeling in the LIPUS + ASCs group as compared with the other groups. Synchrotron radiation micro-computed tomography showed that the LIPUS + ASCs group significantly increased bone volume fraction, trabecular thickness, and trabecular number at the healing site as compared with the other groups at postoperative 8 weeks ( P < .05 for all). Histologically, immunohistochemical staining confirmed that the transplanted mCherry-ASCs can differentiate into osteoblasts and fibrochondrocytic-like cells. Meanwhile, as compared with the other groups, the LIPUS + ASCs group showed more formation and maturity of the fibrocartilage layer and new bone at postoperative weeks 8 and 16 ( P < .05 for all). Biomechanically, the LIPUS + ASCs group showed significantly higher failure load and stiffness versus the other groups at postoperative weeks 8 and 16 ( P < .05 for all).

Conclusion: Autologous ASC transplantation stimulated with LIPUS can result in superior B-T healing quality when compared with LIPUS or ASCs alone.

Clinical Relevance: This study demonstrates the effectiveness of using ASC transplantation stimulated with LIPUS for B-T healing and provides a foundation for future clinical studies.
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http://dx.doi.org/10.1177/0363546518820324DOI Listing
March 2019

Cluster Synchronization for Neutral Stochastic Delay Networks via Intermittent Adaptive Control.

IEEE Trans Neural Netw Learn Syst 2019 Nov 15;30(11):3246-3259. Epub 2019 Feb 15.

This paper studies the problem of cluster synchronization at exponential rates in both the mean square and almost sure senses for neutral stochastic coupled neural networks with time-varying delay via a periodically intermittent pinning adaptive control strategy. The network topology can be symmetric or asymmetric, with each network node being described by neutral stochastic delayed neural networks. When considering the exponential stabilization in the mean square sense for neutral stochastic delay system, the delay integral inequality approach is used to circumvent the obstacle arising from the coexistence of random disturbance, neutral item, and time-varying delay. The almost surely exponential stabilization is also analyzed with the nonnegative semimartingale convergence theorem. Sufficient criteria on cluster synchronization at exponential rates in both the mean square and almost sure senses of the underlying networks under the designed control scheme are derived. The effectiveness of the obtained theoretical results is illustrated by two examples.
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http://dx.doi.org/10.1109/TNNLS.2018.2890269DOI Listing
November 2019

Three-dimensional characterization of the microstructure in rabbit patella-patellar tendon interface using propagation phase-contrast synchrotron radiation microtomography.

J Synchrotron Radiat 2018 Nov 25;25(Pt 6):1833-1840. Epub 2018 Oct 25.

Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, People's Republic of China.

Understanding the three-dimensional ultrastructure morphology of tendon-to-bone interface may allow the development of effective therapeutic interventions for enhanced interface healing. This study aims to assess the feasibility of propagation phase-contrast synchrotron radiation microtomography (PPC-SRµCT) for three-dimensional characterization of the microstructure in rabbit patella-patellar tendon interface (PPTI). Based on phase retrieval for PPC-SRµCT imaging, this technique is capable of visualizing the three-dimensional internal architecture of PPTI at a cellular high spatial resolution including bone and tendon, especially the chondrocytes lacuna at the fibrocartilage layer. The features on the PPC-SRµCT image of the PPTI are similar to those of a histological section using Safranin-O staining/fast green staining. The three-dimensional microstructure in the rabbit patella-patellar tendon interface and the spatial distributions of the chondrocytes lacuna and their quantification volumetric data are displayed. Furthermore, a color-coding map differentiating cell lacuna in terms of connecting beads is presented after the chondrocytes cell lacuna was extracted. This provides a more in-depth insight into the microstructure of the PPTI on a new scale, particularly the cell lacuna arrangement at the fibrocartilage layer. PPC-SRµCT techniques provide important complementary information to the conventional histological method for characterizing the microstructure of the PPTI, and may facilitate in investigations of the repair mechanism of the PPTI after injury and in evaluating the efficacy of a different therapy.
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http://dx.doi.org/10.1107/S160057751801353XDOI Listing
November 2018

Pinning impulsive synchronization for stochastic reaction-diffusion dynamical networks with delay.

Neural Netw 2018 Oct 1;106:281-293. Epub 2018 Aug 1.

School of Electrical and Electronic Engineering, The University of Adelaide, SA 5005, Australia. Electronic address:

This paper considers the problem of the asymptotic synchronization in mean square for stochastic reaction-diffusion complex dynamical networks with infinite delay driven by the Wiener processes in the infinite dimensional space under the pinning impulsive control. Two types of the impulsive controllers are proposed: the first is a single pinning impulsive controller on the first node, and the second is the pinning impulsive controller on a small portion of the network nodes. By using the variation-of-constant formula and the fixed point theorem, the asymptotic behavior of impulsive differential equations with infinite delay is first analyzed. Then, by introducing some operators in the abstract space, the networks are transformed into a set of stochastic coupled impulsive partial differential equations in Hilbert space. Under these two pinning impulsive control types, the asymptotic stability in mean square of stochastic coupled partial differential equations is examined by Lyapunov function approach and the comparison principle. The asymptotic synchronization in mean square of stochastic reaction-diffusion dynamical networks can be realized for these two pinning impulsive control schemes. One example is provided to present the potential application of the theoretic results obtained.
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http://dx.doi.org/10.1016/j.neunet.2018.07.009DOI Listing
October 2018

Oxidation of β-blockers by birnessite: Kinetics, mechanism and effect of metal ions.

Chemosphere 2018 Mar 6;194:588-594. Epub 2017 Dec 6.

Program for the Environment and Sustainability, Department of Environmental and Occupational Health, School of Public Health, Texas A&M University, College Station, TX 77843, USA.

Manganese dioxides are ubiquitous in natural waters, soils, and sediments and play an important role in oxidative transformation of organic pollutants. This work presents the kinetics of the oxidation of selected β-blockers, betaxolol, metoprolol, and atenolol by birnessite (δ-MnO) as a function of concentration of the β-blocker, dosage of δ-MnO, and solution pH. The values of pseudo-first-order rate constants (k) of β-blockers decreased in the order betaxolol > atenolol > metoprolol, which was positively correlated with their acid dissociation constants (K). Effect of series of metal ions (Fe, Cr, Al, Pb, Cu, Zn, Ni, Cd, Mg, and Ca) on the degradation of β-blockers by δ-MnO was systematically examined. All of these metal ions inhibited the oxidation reaction under the same constant ionic strength. The inhibition efficiency was positively correlated with the logarithm of stability constant of metal ions in aqueous solution (logK). By LC-ESI-MS/MS analyses, the oxidation of β-blockers primarily involved hydroxylation and cleavage of the parent molecules to the short branched chain compounds. An electron transfer mechanism for the oxidation of β-blockers by δ-MnO was proposed. The oxidation was initiated by the electron transfer from the nonbonding electrons on nitrogen (N-electrons) of β-blockers to δ-MnO, followed by transformation of radical intermediates. These findings will help to understand the oxidation processes of β-blockers and predict the effect of metal ions on the removal of pollutants by δ-MnO in the environment.
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http://dx.doi.org/10.1016/j.chemosphere.2017.12.015DOI Listing
March 2018

A full-spectrum photocatalyst with strong near-infrared photoactivity derived from synergy of nano-heterostructured Er-doped multi-phase oxides.

Nanoscale 2017 Dec;9(47):18940-18950

Key Laboratory of Pulp & Paper Science and Technology (Qilu University of Technology), Ministry of Education, Jinan, Shandong 250353, China.

The development of full-spectrum photocatalysts active in the near-infrared (NIR) region has gained increasing attention in the photodegradation of organic pollutants. Herein, we designed a full-spectrum photocatalyst with strong NIR photoactivity based on the synergy of Er-doped ZnO-CuO-ZnAlO multi-phase oxides (Er-doped Zn/Cu/Al-MPO) via the formation of n-p-n double heterojunctions. The photocatalyst was prepared by synthesizing nanosheets of a Zn/Cu/Al/Er hydrotalcite-like compound (Zn/Cu/Al/Er-HLC) with a co-precipitation method followed by calcination of the nanosheets at 800 °C. The as-prepared Er-doped Zn/Cu/Al-MPO inherits the nanosheet morphology of Zn/Cu/Al/Er-HLC, and displays over-doubled photoactivity in the entire ultraviolet (UV), visible and NIR regions compared to undoped Zn/Cu/Al-MPO. The excellent photocatalytic activity of Er-doped Zn/Cu/Al-MPO, especially its strong NIR photoactivity, is ascribed to its Er-doped CuO-involved multi-crystalline phase heterostructure, i.e., n-p-n double heterojunctions, which does not only offer an enhanced NIR absorption but also promotes the separation of photogenerated charge carriers. Importantly, the synergy of all the parts of the n-p-n double heterojuctions plays an important role in interface band structure regulation for the enhancement of the photocatalytic properties of Er-doped Zn/Cu/Al-MPO. This work has demonstrated the feasibility of utilizing hydrotalcite-like precursors in the design of full-spectrum photocatalysts active in the NIR region.
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http://dx.doi.org/10.1039/c7nr08090eDOI Listing
December 2017

Anthraquinones from the saline-alkali plant endophytic fungus Eurotium rubrum.

J Antibiot (Tokyo) 2017 12 11;70(12):1138-1141. Epub 2017 Oct 11.

College of Life Sciences, Shandong Normal University, Jinan, China.

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http://dx.doi.org/10.1038/ja.2017.121DOI Listing
December 2017

Low-Intensity Pulsed Ultrasound Stimulation for Tendon-Bone Healing: A Dose-Dependent Study.

Am J Phys Med Rehabil 2018 04;97(4):270-277

From the Department of Sports Medicine, Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China (HL, FL, CC, ZW, HC, JQ, TZ, DX); and Department of Spine Surgery, Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China (JH).

Objective: The aim of the study was to evaluate the dosage effect of low-intensity pulsed ultrasound stimulation on tendon-bone healing.

Design: Standard partial patellectomies were performed on 120 mature New Zealand rabbits randomly assigned into the following three groups: a control group (daily mock sonication, 20 mins), a qd group (daily ultrasonication, 20 mins), and a bid group (ultrasonication twice a day, 20 mins each time). The rabbits were killed 8 or 16 wks postoperatively, and the microarchitectural, histological, and mechanical properties of the patella-patellar tendon interface were evaluated.

Results: Microcomputed tomography analysis showed that the bid group exhibited more new bone formation and mineralization than the other groups in the tendon-bone healing position at both 8 and 16 wks postoperatively. Histological assessments confirmed that the bid group exhibited a significantly better patella-patellar tendon interface than the other groups, as shown by the increased formation and remodeling of newly formed bone and a fibrocartilage layer. The biomechanical properties of the regenerated patella-patellar tendon interface significantly improved in the bid group.

Conclusions: Low-intensity pulsed ultrasound stimulation treatment twice a day was more effective than the once-a-day treatment on tendon-bone healing.
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http://dx.doi.org/10.1097/PHM.0000000000000844DOI Listing
April 2018

Initiation Timing of Low-Intensity Pulsed Ultrasound Stimulation for Tendon-Bone Healing in a Rabbit Model.

Am J Sports Med 2016 Oct 29;44(10):2706-2715. Epub 2016 Jun 29.

Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China Department of Spine Surgery, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China

Background: Low-intensity pulsed ultrasound stimulation (LIPUS) has been proven to be a beneficial biophysical therapy for tendon-bone (T-B) healing. However, the optimal time to initiate LIPUS treatment has not been determined yet. LIPUS initiated at different stages of the inflammatory phase may profoundly affect T-B healing.

Purpose: An established rabbit model was used to preliminarily investigate the effect of LIPUS initiation timing on T-B healing.

Study Design: Controlled laboratory study.

Methods: A total of 112 mature rabbits that underwent partial patellectomy were randomly assigned to 4 groups: daily mock sonication (control group) and daily ultrasonication started immediately postoperatively (immediate group), on postoperative day 7 (7-day delayed group), or on postoperative day 14 (14-day delayed group). Peripheral leukocyte counts at the inflammatory phase were used to assess postoperative inflammation. The rabbits were sacrificed at 8 or 16 weeks postoperatively for microarchitectural, histological, and mechanical evaluations of the patella-patellar tendon (PPT) junction.

Results: The biomechanical properties of the PPT junction were significantly improved in the LIPUS-treated groups. Significantly higher ultimate strength and stiffness were seen in the 7-day delayed group compared with the other groups at 8 weeks postoperatively (P < .05 for all). Newly formed bone expansion from the remaining patella in the ultrasonic treatment groups was significantly increased and remodeled compared with the control group. Micro-computed tomography analysis showed that the 7-day delayed group had significantly more bone volume and bone mineral content at the interface as compared with the other groups at 8 weeks postoperatively (P < .05 for all). Histologically, the ultrasonic treatment groups exhibited a significantly better PPT junction, as shown by more formation and remodeling of the fibrocartilage layer and newly formed bone. Additionally, peripheral leukocyte counts displayed a significant increase from postoperative day 1 to day 3 in the immediate group as compared with the other groups. Furthermore, postoperative hydrarthrosis was more likely in the immediate group.

Conclusion: LIPUS started at postoperative day 7 had a more prominent effect on T-B healing compared with the other treatment regimens in this study.

Clinical Relevance: The findings of the study may help optimize the initiation timing of LIPUS for T-B healing.
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http://dx.doi.org/10.1177/0363546516651863DOI Listing
October 2016

Exponential Stability for Neutral Stochastic Markov Systems With Time-Varying Delay and Its Applications.

IEEE Trans Cybern 2016 06;46(6):1350-62

In this paper, the exponential stability in p th( p > 1 )-moment for neutral stochastic Markov systems with time-varying delay is studied. The derived stability conditions comprise two forms: 1) the delay-independent stability criteria which are obtained by establishing an integral inequality and 2) the delay-dependent stability criteria which are captured by using the theory of the functional differential equations. As its applications, the obtained stability results are used to investigate the exponential stability in p th( p > 1 )-moment for the neutral stochastic neural networks with time-varying delay and Markov switching, and the globally exponential adaptive synchronization for the neutral stochastic complex dynamical systems with time-varying delay and Markov switching, respectively. On the delay-independent criteria, sufficient conditions are given in terms of M -matrix and thus are easy to check. The delay-dependent criteria are presented in the forms of the algebraic inequalities, and the least upper bound of the time-varying delay is also provided. The primary advantages of these obtained results over some recent and similar works are that the differentiability or continuity of the delay function is not required, and that the difficulty stemming from the presence of the neutral item and the Markov switching is overcome. Three numerical examples are provided to examine the effectiveness and potential of the theoretic results obtained.
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http://dx.doi.org/10.1109/TCYB.2015.2442274DOI Listing
June 2016

Exponential Synchronization for Markovian Stochastic Coupled Neural Networks of Neutral-Type via Adaptive Feedback Control.

IEEE Trans Neural Netw Learn Syst 2017 07 12;28(7):1618-1632. Epub 2016 Apr 12.

In this paper, we investigate the adaptive exponential synchronization in both the mean square and the almost sure senses for an array of N identical Markovian stochastic coupled neural networks of neutral-type with time-varying delay and random coupling strength. The generalized Lyapunov theorem of the exponential stability in the mean square for the neutral stochastic Markov system with the time-varying delay is first established. The time-varying delay in the system is assumed to be a bounded measurable function. Then, sufficient conditions to guarantee the exponential synchronization in the mean square for the underlying system are developed under an adaptive feedback controller, which are given in terms of the M -matrix and the algebraic inequalities. Under the same conditions, the almost sure exponential synchronization is also presented. A numerical example is given to show the effectiveness and potential of the proposed theoretical results.
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http://dx.doi.org/10.1109/TNNLS.2016.2546962DOI Listing
July 2017

The effect of low-intensity pulsed ultrasound on bone-tendon junction healing: Initiating after inflammation stage.

J Orthop Res 2016 10 18;34(10):1697-1706. Epub 2016 Feb 18.

Department of Spine Surgery, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.

The purpose of this study was to explore the effect of low-intensity pulsed ultrasound (LIPUS) treatment initiating after inflammation stage on the process of bone-tendon junction (BTJ) healing in a rabbit model. Thirty-six rabbits undergoing partial patellectomy were randomly divided into two groups: control and LIPUS. The period of initial inflammatory stage is 2 weeks. So LIPUS treatment was initiated at postoperative week 2 and continued until the patella-patellar tendon (PPT) complexes were harvested at postoperative weeks 4, 8, and 16. At each time point, the PPT complexes were harvested for qRT-PCR, histology, radiographs, synchroton radiation micro computed tomography (SR-µCT), and biomechanical testing. The qRT-PCR results showed that LIPUS treatment beginning at postoperative week 2 played an anti-inflammatory role in BTJ healing. Histologically, the LIPUS group showed more advanced remodeling of the lamellar bone and marrow cavity than the control group. The area and length of the new bone in the LIPUS group were significantly greater than the control group at postoperative weeks 8 and 16. SR-µCT demonstrated that new bone formation and remodeling in the LIPUS group were more advanced than the control group. Biomechanical test results demonstrated that the failure load, ultimate strength and energy at failure were significantly higher than those of the control group. In conclusion, LIPUS treatment beginning at postoperative week 2 was able to accelerate bone formation during the bone-tendon junction healing process and significantly improved the healing quality of BTJ injury. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1697-1706, 2016.
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http://dx.doi.org/10.1002/jor.23180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6084317PMC
October 2016

Up-regulated Circulating miR-106a by DNA Methylation Promised a Potential Diagnostic and Prognostic Marker for Gastric Cancer.

Anticancer Agents Med Chem 2016 ;16(9):1093-100

Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou, 215006, China.

Previous studies suggested that abnormal miRNA expression was a significant characteristic of malignant tumors. We aimed to explore the role of miR-106a as the potential diagnostic and prognostic biomarker in gastric cancer (GC). Firstly, the expression level of miR-106a was detected by qPCR in 28 pairs of GC cancer tissues and adjacent tissues, 48 pairs of plasma samples before and after operation from GC patients, and 22 plasma samples from healthy controls. It had revealed that the level of miR-106a in tumor tissues (2.700±2.565) was significantly higher compared to adjacent tissues (1.321±0.904) (p<0.05). Besides, the expression level of miR-106a in plasma of GC (9.479±5.595) was significantly up-regulated compared with healthy controls (2.594±2.329) (p<0.05), while a remarkable decline of miR- 106a expression was observed in plasma of GC patients after gastrectomy. Further statistic data showed high miR-106a expression was closely related to the degree of lymphatic metastasis and TNM staging of GC. We also applied ROC curve in order to evaluate miR-106a as a diagnostic marker for GC patients. As a result, the sensitivity and specificity were 60.4%, 68.2% in tissue samples and 72.9%, 63.6% in plasma samples, respectively. At last, we explored the methylation status of miR-106a promoter in 28 paired GC tissues through methylation-specific PCR (MSP), the result showed that the methylation rate was 53.6% in cancer tissues and 85.7% in adjacent tissues. Moreover, the result indicated that promoter hypomethylation of miR- 106a is related to its high expression. Our research indicated that miR-106a might serve as a potential prognostic indicator in progressive GC and up-regulated circulating miR-106a by promoter hypomethylation, might be proposed as a candidate diagnostic and prognostic indicator for GC.
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http://dx.doi.org/10.2174/1871520615666150716110657DOI Listing
June 2017

Effects of low-intensity pulsed ultrasound on new trabecular bone during bone-tendon junction healing in a rabbit model: a synchrotron radiation micro-CT study.

PLoS One 2015 15;10(4):e0124724. Epub 2015 Apr 15.

Department of Spine Surgery, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.

This study was designed to evaluate the effects of low-intensity pulsed ultrasound on bone regeneration during the bone-tendon junction healing process and to explore the application of synchrotron radiation micro computed tomography in three dimensional visualization of the bone-tendon junction to evaluate the microarchitecture of new trabecular bone. Twenty four mature New Zealand rabbits underwent partial patellectomy to establish a bone-tendon junction injury model at the patella-patellar tendon complex. Animals were then divided into low-intensity pulsed ultrasound treatment (20 min/day, 7 times/week) and placebo control groups, and were euthanized at week 8 and 16 postoperatively (n = 6 for each group and time point). The patella-patellar tendon specimens were harvested for radiographic, histological and synchrotron radiation micro computed tomography detection. The area of the newly formed bone in the ultrasound group was significantly greater than that of control group at postoperative week 8 and 16. The high resolution three dimensional visualization images of the bone-tendon junction were acquired by synchrotron radiation micro computed tomography. Low-intensity pulsed ultrasound treatment promoted dense and irregular woven bone formation at week 8 with greater bone volume fraction, number and thickness of new trabecular bone but with lower separation. At week 16, ultrasound group specimens contained mature lamellar bone with higher bone volume fraction and thicker trabeculae than that of control group; however, there was no significant difference in separation and number of the new trabecular bone. This study confirms that low-intensity pulsed ultrasound treatment is able to promote bone formation and remodeling of new trabecular bone during the bone-tendon junction healing process in a rabbit model, and the synchrotron radiation micro computed tomography could be applied for three dimensional visualization to quantitatively evaluate the microarchitecture of new bone in bone-tendon junction.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124724PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4398446PMC
January 2016

A promising gene delivery system developed from PEGylated MoS2 nanosheets for gene therapy.

Nanoscale Res Lett 2014 27;9(1):587. Epub 2014 Oct 27.

Department of Radiology, the First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, China.

A new class of two-dimensional (2D) nanomaterial, transition metal dichalcogenides (TMDCs) such as MoS2, MoSe2, WS2, and WSe2 which have fantastic physical and chemical properties, has drawn tremendous attention in different fields recently. Herein, we for the first time take advantage of the great potential of MoS2 with well-engineered surface as a novel type of 2D nanocarriers for gene delivery and therapy of cancer. In our system, positively charged MoS2-PEG-PEI is synthesized with lipoic acid-modified polyethylene glycol (LA-PEG) and branched polyethylenimine (PEI). The amino end of positively charged nanomaterials can bind to the negatively charged small interfering RNA (siRNA). After detection of physical and chemical characteristics of the nanomaterial, cell toxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Polo-like kinase 1 (PLK1) was investigated as a well-known oncogene, which was a critical regulator of cell cycle transmission at multiple levels. Through knockdown of PLK1 with siRNA carried by novel nanovector, qPCR and Western blot were used to measure the interfering efficiency; apoptosis assay was used to detect the transfection effect of PLK1. All results showed that the novel nanocarrier revealed good biocompatibility, reduced cytotoxicity, as well as high gene-carrying ability without serum interference, thus would have great potential for gene delivery and therapy.
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http://dx.doi.org/10.1186/1556-276X-9-587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4216190PMC
November 2014

Five-electrode direct current suppressor-detector combiner for ion chromatography: an integration of eluent suppression and resistance detection.

Analyst 2011 Mar 6;136(5):901-3. Epub 2011 Jan 6.

The Key Laboratory of Analytical Science of Xiamen University, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.

An integration of eluent suppressor and resistance detector is proposed based upon the application of a common anode (or cathode), a common ion-exchange membrane, and two independent constant current sources.
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http://dx.doi.org/10.1039/c0an00839gDOI Listing
March 2011

A four-electrode microconstant direct current resistance detector for ion chromatography applying ion-exchange membrane and porous electrode.

Talanta 2010 Sep 4;82(4):1364-70. Epub 2010 Aug 4.

The Key Laboratory of Analytical Science of Xiamen University, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

A four-electrode microconstant direct current resistance detector for ion chromatography not sensitive to the effects of electrode polarization, capacitance, and electrolysis by-products is proposed. A constant current of microampere magnitude is applied across the current electrodes of the four-electrode device, and the voltage responses between the detection probes are directly picked up by a high input impedance instrumentation amplifier. The ion-exchange membranes, which separate the detection chamber from the electrolysis chambers, enable the measurement of solution resistance free of the interference of electrolysis by-products. Two resin beds in the detection chamber serve as ion conductors while reduce the dead volume of the detector. Recycled detection effluent supplies water for the electrolysis reactions at the current electrodes to sustain constant current in solution. The porous detection probes provide microchannel for the flowing solution while indicating signals. Owing to the constant current excitation, the electronics setup becomes simple. The cell configuration, operating principle, electronics, and error analysis of this detection mode are discussed along with their use for suppressed anion chromatography. Experimental data show that this four-electrode direct current detection mode is comparable to conventional two-electrode alternating current method.
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http://dx.doi.org/10.1016/j.talanta.2010.07.003DOI Listing
September 2010

Motivations and attitudes affecting high school students' choice of foreign language.

Adolescence 2003 ;38(149):161-70

Department of Modern Languages and Literature, Eastern Washington University, Cheney, Washington, USA.

There are some foreign languages that enjoy great status in the United States, while other foreign languages are rarely represented at the high school level. The present study explored the following questions: Why do students choose to take a particular foreign language? Do students gravitate toward it because it is widely thought to be the easiest language to learn or because they perceive greater career opportunities with proficiency in this particular language, or is it simply because there are more classes offered? As long as foreign language study is elective in high schools and as long as a variety of languages are offered, the answers to these questions will remain important for foreign language educators, especially in schools where the various language programs compete with one another for student enrollments and the programs' ultimate survival.
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October 2003