Publications by authors named "Hua Zhang"

3,394 Publications

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The Mediating Roles of Core Self-Evaluation and Career Exploration in the Association Between Proactive Personality and Job Search Clarity.

Front Psychol 2021 4;12:609050. Epub 2021 Jun 4.

School of International Nursing, Hainan Medical University, Haikou, China.

In recent years, university students' employment has become an increasingly prominent problem worldwide. Improving the job search clarity of students is a great way to boost job-hunting success. Proactive personality may predict job search clarity through the mediating effects of core self-evaluation and career exploration. However, few studies have explored this relationship and the mediating roles of core self-evaluation and career exploration. To identify the relationship between a proactive personality and job search clarity and the mediating roles of core self-evaluation and career exploration, a cross-sectional survey was conducted. A total of 495 students majoring in nursing completed the questionnaire which consisted of the proactive personality scale, core self-evaluation scale, career exploration survey, and job search clarity scale. Correlation analyses and mediation analyses were conducted using SPSS 24.0 and AMOS 24.0 respectively. This study showed that students higher on the proactive personality scale were more likely to perform better on job search clarity. Core self-evaluation and career exploration have fully mediating effects on the relationship between a proactive personality and job search clarity. Therefore, interventions concentrated on cultivating proactive personality, improving core self-evaluation, and strengthening career exploration would be necessary for increasing job search clarity.
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http://dx.doi.org/10.3389/fpsyg.2021.609050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211878PMC
June 2021

Wet-Chemical Synthesis and Applications of Semiconductor Nanomaterial-Based Epitaxial Heterostructures.

Nanomicro Lett 2019 Oct 15;11(1):86. Epub 2019 Oct 15.

Center for Programmable Materials, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.

Semiconductor nanomaterial-based epitaxial heterostructures with precisely controlled compositions and morphologies are of great importance for various applications in optoelectronics, thermoelectrics, and catalysis. Until now, various kinds of epitaxial heterostructures have been constructed. In this minireview, we will first introduce the synthesis of semiconductor nanomaterial-based epitaxial heterostructures by wet-chemical methods. Various architectures based on different kinds of seeds or templates are illustrated, and their growth mechanisms are discussed in detail. Then, the applications of epitaxial heterostructures in optoelectronics, catalysis, and thermoelectrics are described. Finally, we provide some challenges and personal perspectives for the future research directions of semiconductor nanomaterial-based epitaxial heterostructures.
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http://dx.doi.org/10.1007/s40820-019-0317-6DOI Listing
October 2019

Minute Cu coupling with HCO for efficient degradation of acetaminophen via HO activation.

Ecotoxicol Environ Saf 2021 Jun 16;221:112422. Epub 2021 Jun 16.

School of Geography and Environment, Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China.

Homogeneous Cu-mediated activation of HO has been widely applied for the removal of organic contaminants, but fairly high dosage of Cu is generally required and may cause secondary pollution. In the present study, minute Cu (2.5 μM) catalyzed HO exhibited excellent efficiency in degradation of organic pollutants with the assistant of naturally occurring level HCO (1 mM). In a typical case, acetaminophen (ACE) was completely eliminated within 10 min which followed the pseudo-first-order kinetics. Singlet oxygen and superoxide radical rather than traditionally identified hydroxyl radical were the predominant reactive oxygen species (ROS) responsible for ACE degradation. Meanwhile, Cu was deduced through Cu and p-hydroxybenzoic acid formation analysis. CuCO(aq) was the main complex with high reactivity for the activation of HO to form ROS and Cu. The removal efficiency of ACE depended on the operating parameters, such as Cu, HCO and HO dosage, solution initial pH. The presence of Cl, HPO, humic acid were found to retard ACE removal while other anions such as SO and NO had no obvious effect. ACE exhibited lower degradation efficiency in real water matrices than that in ultra-pure water. Nevertheless, 58-100% of ACE was removed from domestic wastewater, lake water and tap water within 60 min. Moreover, eight intermediate products were identified and the possible degradation pathways of ACE were proposed. Additionally, other typical organic pollutants including bisphenol A, norfloxacin, lomefloxacin hydrochloride and sulfadiazine, exhibited great removal efficiency in the Cu/HO/HCO system.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112422DOI Listing
June 2021

Effects of temperature on the growth and carbohydrate production of three benthic dinoflagellate species from Hainan Island, South China Sea.

Ecotoxicology 2021 Jun 17. Epub 2021 Jun 17.

College of Life Science and Technology, Jinan University, Guangzhou, China.

Dinoflagellates in the genera Ostreopsis and Gambierdiscus are typical members of the marine benthic community particularly in tropical areas. Their geographic distribution has shown an increasing expansion towards temperate waters recently due to the global warming and climate changes; however, the knowledge is little of how the increasing temperatures might influence the physiological characteristics of Ostreopsis spp. and Gambierdiscus spp. Here, we carried out experiments to understand the effect of different temperatures on the growth, chlorophyll a content, and carbohydrate yield of Chinese strains of Ostreopsis cf. ovata, O. lenticularis, and Gambierdiscus caribaeus. Specifically, seven temperatures (15, 17.5, 20, 25, 30, 32.5, and 35 °C) were set for the two Ostreopsis species and five temperatures (15, 20, 25, 30, and 35 °C) were set for G. caribaeus. Our results suggested that both Ostreopsis (both species were 17.5-32.5 °C) and Gambierdiscus (20-35 °C) could survive a wide range of temperatures, consistent with the record worldwide. Cell density and chlorophyll a content were observed to be higher at high temperatures (30 and 32.5 °C) for both Ostreopsis species whereas G. caribaeus reached the maximum cell density and highest growth rate at 20 °C. Higher carbohydrate yield was detected in the suboptimal temperatures for all three dinoflagellates especially during the decaying phase. Our study reveals the optimal temperatures for the growth of three benthic harmful dinoflagellate species and provides insight into how the increasing temperature will affect their abundance as well as distribution.
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http://dx.doi.org/10.1007/s10646-021-02428-zDOI Listing
June 2021

Chemical constituents from Tinospora sagittata and their biological activities.

Fitoterapia 2021 Jun 12;153:104963. Epub 2021 Jun 12.

School of Biological Science and Technology, University of Jinan, Jinan 250022, China. Electronic address:

Six undescribed low-polarity compounds including three rare 14-methylergostane steroids (1-3), one euphane triterpenoid (4) and two octadecanoic acid ethyl esters (5 and 6), along with ten previously reported terpenyl cometabolites (7-16), were isolated from the stems of Tinospora sagittata. Their structures were determined by detailed spectroscopic analyses and comparison with structurally related known compounds, and all of them have been reported from T. sagittata for the first time. Compounds 4-6 and 16 showed potent in vitro inhibitory activity against the diabetes target α-glucosidase, while compounds 10 and 14 displayed promising antibacterial effect toward Staphylococcus aureus ATCC 25923.
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http://dx.doi.org/10.1016/j.fitote.2021.104963DOI Listing
June 2021

Expression-EEG Bimodal Fusion Emotion Recognition Method Based on Deep Learning.

Comput Math Methods Med 2021 25;2021:9940148. Epub 2021 May 25.

Department of Electrical & Information Engineering, Sichuan Engineering Technical College, Deyang, Sichuan 618000, China.

As one of the key issues in the field of emotional computing, emotion recognition has rich application scenarios and important research value. However, the single biometric recognition in the actual scene has the problem of low accuracy of emotion recognition classification due to its own limitations. In response to this problem, this paper combines deep neural networks to propose a deep learning-based expression-EEG bimodal fusion emotion recognition method. This method is based on the improved VGG-FACE network model to realize the rapid extraction of facial expression features and shorten the training time of the network model. The wavelet soft threshold algorithm is used to remove artifacts from EEG signals to extract high-quality EEG signal features. Then, based on the long- and short-term memory network models and the decision fusion method, the model is built and trained using the signal feature data extracted under the expression-EEG bimodality to realize the final bimodal fusion emotion classification and identification research. Finally, the proposed method is verified based on the MAHNOB-HCI data set. Experimental results show that the proposed model can achieve a high recognition accuracy of 0.89, which can increase the accuracy of 8.51% compared with the traditional LSTM model. In terms of the running time of the identification method, the proposed method can effectively be shortened by about 20 s compared with the traditional method.
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http://dx.doi.org/10.1155/2021/9940148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172283PMC
May 2021

Trophic interactions of reef-associated predatory fishes (Hexagrammos otakii and Sebastes schlegelii) in natural and artificial reefs along the coast of North Yellow Sea, China.

Sci Total Environ 2021 Jun 6;791:148250. Epub 2021 Jun 6.

Research and Development Center for Efficient Utilization of Coastal Bioresources, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, People's Republic of China; Muping Coastal Environment Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, People's Republic of China. Electronic address:

Large reef-associated predatory fishes play important roles in aquatic ecosystem along coast because of their ecological functions and economic values to recreational and commercial fisheries. This study was carried out to assess the function of artificial reefs as alternative habitats for two common reef-associated predatory fishes in the north of Yellow Sea, China, Fat Greenling Hexagrammos otakii and Korean rockfish Sebastes schlegelii. According to the catch per unit effort (CPUE), the biomass of predatory fishes at the artificial reef was comparable (H. otakii) to or higher (S. schlegelii) than the natural reef, highlighting the environmental fitness of the artificial reef. Gut content analysis (GCA) showed that H. otakii preyed primarily on Decapod and Amphipoda, while S. schlegelii exhibited higher dependence on fish (Blinniidae and Gobiidae) and Decapod. Collectively, prey richness and diversity were greater at the natural reef relative to the artificial reef, and prey availability may be different between the two reef types. Stable isotopic analysis (SIA) in conjunction with the Bayesian mixing model (MixSIAR) revealed spatial and interspecific difference on the diet composition of H. otakii and S. schlegelii as well. Based on GCA and MixSIAR result, the habitat-specific effect on the prey availability was confirmed. Additionally, comparisons on trophic niche breadth and niche overlap indicated higher trophic diversity but relatively lower food resources partitioning degree for both species at the natural reef than at the artificial reef. Our results suggest that artificial reefs may harbor a different prey assemblage comparing to natural reef but can support large populations of predatory reef-associated fishes and accommodate their coexistence.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148250DOI Listing
June 2021

Identification of Novel Biomarkers for Evaluating Disease Severity in House-Dust-Mite-Induced Allergic Rhinitis by Serum Metabolomics.

Dis Markers 2021 19;2021:5558458. Epub 2021 May 19.

Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, Hunan, China.

The aim of this study was to identify differences in serum metabolomics profiles of house-dust-mite (HDM)-induced allergic rhinitis (AR) patients compared to controls and to explore novel biomarkers reflecting disease severity. Serum samples were collected from 29 healthy controls and HDM-induced 72 AR patients, including 30 mild patients (MAR) and 42 moderate to severe AR patients (MSAR). Metabolomics detection was performed, and orthogonal partial least square discriminate analysis was applied to assess the differences between AR patients and controls and for subgroups based on disease severity. These analysis results successfully revealed distinct metabolite signatures which distinguished MAR patients and MSAR patients from controls. MSAR patients also could be discriminated from MAR patients based on their metabolic fingerprints. Most observed metabolite changes were related to glycine, serine, and threonine metabolism, pyrimidine metabolism, sphingolipid metabolism, arginine and proline metabolism, and fatty acid metabolism. Levels of sarcosine, sphingosine-1-phosphate, cytidine, and linoleic acid significantly correlated with the total nasal symptom score and visual analogue scale in AR patients. These results suggest that metabolomics profiling may provide novel insights into the pathophysiological mechanisms of HDM-induced AR and contribute to its evaluation of disease severity.
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http://dx.doi.org/10.1155/2021/5558458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154289PMC
May 2021

LncRNAs serve as novel biomarkers for diagnosis and prognosis of childhood ALL.

Biomark Res 2021 Jun 10;9(1):45. Epub 2021 Jun 10.

Institute of Laboratory Medicine, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Key Laboratory of Big Data Mining and Precision Drug Design, School of Medical Technology, Guangdong Medical University, Guangdong Medical University, 523808, Dongguan, China.

Background: Although some studies have demonstrated that lncRNAs are dysregulated in hematopoietic malignancies and may regulate the progression of leukemia, the detailed mechanism underlying tumorigenesis is still unclear. This study aimed to investigate lncRNAs that are differentially expressed in childhood B-cell acute lymphoblastic leukemia (B-ALL) and T-cell acute lymphoblastic leukemia (T-ALL) and their potential roles in the progression of childhood ALL.

Methods: Microarrays were used to detect differentially expressed lncRNAs and mRNAs. Several aberrantly expressed lncRNAs were validated by qRT-PCR. Leukemia-free survival was analyzed using the Kaplan-Meier method with a log-rank test. The co-expression correlations of lncRNAs and mRNAs were determined by Spearman's correlation coefficient. CCK-8 assays and flow cytometry were performed to measure cell proliferation and apoptosis.

Results: We revealed that many lncRNAs were abnormally expressed in B-ALL and T-ALL. LncRNA/mRNA co-expression and the gene locus network showed that dysregulated lncRNAs are involved in diverse cellular processes. We also assessed the diagnostic value of the differentially expressed lncRNAs and confirmed the optimal combination of TCONS_00026679, uc002ubt.1, ENST00000411904, and ENST00000547644 with an area under the curve of 0.9686 [95 % CI: 0.9369-1.000, P < 0.001], with 90.7 % sensitivity and 92.19 % specificity, at a cut-off point of -0.5700 to distinguish childhood B-ALL patients from T-ALL patients, implying that these specific lncRNAs may have potential to detect subsets of childhood ALL. Notably, we found that the 8-year leukemia-free survival of patients with high TCONS_00026679 (p = 0.0081), ENST00000522339 (p = 0.0484), ENST00000499583 (p = 0.0381), ENST00000457217 (p = 0.0464), and ENST00000451368 (p = 0.0298) expression levels was significantly higher than that of patients with low expression levels of these lncRNAs, while patients with high uc002ubt.1 (p = 0.0499) and ENST00000547644 (p = 0.0451) expression levels exhibited markedly shorter 8-year leukemia-free survival. In addition, some lncRNAs were found to play different roles in cell proliferation and apoptosis in T-ALL and B-ALL.

Conclusions: Dysregulated lncRNAs involved in different regulatory mechanisms underlying the progression of childhood T-ALL and B-ALL might serve as novel biomarkers to distinguish ALL subsets and indicate poor outcomes.
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http://dx.doi.org/10.1186/s40364-021-00303-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193891PMC
June 2021

Comprehensive Analysis of the Diagnosis and Treatment of Tracheobronchial Foreign Bodies in Children.

Ear Nose Throat J 2021 Jun 10:1455613211023019. Epub 2021 Jun 10.

Department of Otolaryngology-Head and Neck Surgery, 117747Yantai Yuhuangding Hospital, Qingdao University, Yantai, Shandong, China.

Objective: To analyze the factors influencing the diagnosis and treatment of tracheobronchial foreign bodies (TFBs) in children.

Methods: The clinical data of 300 consecutive children with suspected TFBs who were admitted to our department between January 2016 and December 2019 were retrospectively collected, including demographics, diagnosis, history of foreign body inhalation, preoperative chest computed tomography (CT) findings, duration of foreign body retention, time from admission to operation, operation duration, duration of hospitalization, and complications.

Results: Among the 300 cases, the male:female ratio was 193:107, and the age range was 6 months to 12 years (median age: 19 months). A total of 291 cases (97.0%) involved TFBs confirmed by rigid bronchoscopy, while the other 9 cases (3.0%) involved bronchopneumonia. The diagnostic accuracy, sensitivity, and specificity of a history of foreign body inhalation and chest CT were 96.0%, 98.6%, and 11.1% and 97.7%, 97.6%, and 100%, respectively. The duration of hospitalization, time from admission to operation, and operation duration were all related to bronchopneumonia ( < .05).

Conclusions: A detailed history, adequate physical examination, and preoperative imaging examination help improve the diagnostic accuracy. Preoperative bronchopneumonia in children with TFBs will increase the surgical risks and treatment costs, prolonging the duration of hospitalization.
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http://dx.doi.org/10.1177/01455613211023019DOI Listing
June 2021

Does liver cirrhosis affect the surgical outcome of primary colorectal cancer surgery? A meta-analysis.

World J Surg Oncol 2021 Jun 9;19(1):167. Epub 2021 Jun 9.

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Purpose: The purpose of this meta-analysis was to evaluate the effect of liver cirrhosis (LC) on the short-term and long-term surgical outcomes of colorectal cancer (CRC).

Methods: The PubMed, Embase, and Cochrane Library databases were searched from inception to March 23, 2021. The Newcastle-Ottawa Scale (NOS) was used to assess the quality of enrolled studies, and RevMan 5.3 was used for data analysis in this meta-analysis. The registration ID of this current meta-analysis on PROSPERO is CRD42021238042.

Results: In total, five studies with 2485 patients were included in this meta-analysis. For the baseline information, no significant differences in age, sex, tumor location, or tumor T staging were noted. Regarding short-term outcomes, the cirrhotic group had more major complications (OR=5.15, 95% CI=1.62 to 16.37, p=0.005), a higher re-operation rate (OR=2.04, 95% CI=1.07 to 3.88, p=0.03), and a higher short-term mortality rate (OR=2.85, 95% CI=1.93 to 4.20, p<0.00001) than the non-cirrhotic group. However, no significant differences in minor complications (OR=1.54, 95% CI=0.78 to 3.02, p=0.21) or the rate of intensive care unit (ICU) admission (OR=0.76, 95% CI=0.10 to 5.99, p=0.80) were noted between the two groups. Moreover, the non-cirrhotic group exhibited a longer survival time than the cirrhotic group (HR=2.96, 95% CI=2.28 to 3.85, p<0.00001).

Conclusion: Preexisting LC was associated with an increased postoperative major complication rate, a higher rate of re-operation, a higher short-term mortality rate, and poor overall survival following CRC surgery.
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http://dx.doi.org/10.1186/s12957-021-02267-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191032PMC
June 2021

Chemical Vapor Deposition of Superconducting FeTeSe Nanosheets.

Nano Lett 2021 Jun 9. Epub 2021 Jun 9.

CINTRA CNRS/NTU/THALES, UMI 3288, Research Techno Plaza, Singapore, 637553, Singapore.

FeTeSe, a promising layered material used to realize Majorana zero modes, has attracted enormous attention in recent years. Pulsed laser deposition (PLD) and molecular-beam epitaxy (MBE) are the routine growth methods used to prepare FeTeSe thin films. However, both methods require high-vacuum conditions and polished crystalline substrates, which hinder the exploration of the topological superconductivity and related nanodevices of this material. Here we demonstrate the growth of the ultrathin FeTeSe superconductor by a facile, atmospheric pressure chemical vapor deposition (CVD) method. The composition and thickness of the two-dimensional (2D) FeTeSe nanosheets are well controlled by tuning the experimental conditions. The as-prepared FeTeSe nanosheet exhibits an onset superconducting transition temperature of 12.4 K, proving its high quality. Our work offers an effective strategy for preparing the ultrathin FeTeSe superconductor, which could become a promising platform for further study of the unconventional superconductivity in the FeTeSe system.
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http://dx.doi.org/10.1021/acs.nanolett.1c01577DOI Listing
June 2021

Ferrous hemoglobin and hemoglobin-based oxygen carriers acting as a peroxidase can inhibit oxidative damage to endothelial cells caused by hydrogen peroxide.

Artif Organs 2021 Jun 8. Epub 2021 Jun 8.

College of Life Science, Northwest University, Xi'an, P.R. China, 710069.

Oxidative damage caused by the ferryl hemoglobin is one of the major clinical adverse reactions of hemoglobin-based oxygen carriers (HBOCs), while the production of reactive oxygen species in a pathological state can oxidize hemoglobin (HbFe ) to ferryl Hb, which can then enter the pseudoperoxidase cycle, making hemoglobin highly toxic. In this study, we found that ferrous hemoglobin and polymerized porcine hemoglobin (one of the HBOCs) have the peroxidase activity different from the pseudoperoxidase activity of ferric hemoglobin. Ferrous hemoglobin can catalyze the reaction of tyrosine (Tyr) with hydrogen peroxide. In addition, the results also indicated that ferrous hemoglobin and pPolyHb have a strong inhibitory effect on the pseudoperoxidase activity of ferric hemoglobin. Therefore, hydrogen peroxide was consumed in a large amount, which greatly prevented hemoglobin from becoming oxidized and entering the pseudoperoxidase cycle, thus inhibiting ferryl Hb toxicity. We further cultured human umbilical vein endothelial cells and monitored cell morphology, viability, cell cycle, apoptosis, LDH release, and MDA formation when incubated with H O , Tyr, and HbFe . HbFe and pPolyHb reduced cell cycle arrest, apoptosis, LDH release, and MDA formation. These results showed that reducing oxidative damage induced by H O and converted hemoglobin from a molecule that is toxic to one that inhibits oxidative damage, suggesting a new strategy for development of a safer HBOCs.
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http://dx.doi.org/10.1111/aor.14009DOI Listing
June 2021

MiR-27a-3p enhances the cisplatin sensitivity in hepatocellular carcinoma cells through inhibiting PI3K/Akt pathway.

Biosci Rep 2021 Jun 7. Epub 2021 Jun 7.

Xinjiang Medical University Affiliated First Hospital, Urumqi, China.

MicroRNAs (miRNAs) play an important role in drug-resistance, and it's reported that MiR-27a-3p regulated the sensitivity of cisplatin in breast cancer, lung cancer and ovarian cancer. However, the relationship between miR-27a-3p and chemosensitivity of cisplatin in HCC was unclear, especially the underlying mechanism was unknown. In present study, we analyzed miR-27a-3p expression levels in 372 tumor tissues and 49 adjacent tissues in HCC samples from TCGA database, and found that the miR-27a-3p was downregulated in HCC tissues. The level of miR-27a-3p was associated with metastasis, Child-Pugh grade and race. MiR-27a-3p was regarded as a favorable prognosis indicator for HCC patients. Then, miR-27a-3p was overexpressed in HepG2 cell, and was knockdown in PLC cell. Next, we conducted a series of vitro assays, including MTT, apoptosis and cell cycle assays to observe the biological changes. Further, inhibitor rate and apoptosis rate were detected with pre- and post-cisplatin treatment in HCC. The results showed that overexpression of miR-27a-3p repressed the cell viability, promoted apoptosis and increased the percentage of cells in phase G0/G1 phase. Importantly, overexpression of miR-27a-3p significantly increased the inhibitor rate and apoptosis rate with cisplatin intervention. Besides, we found that miR-27a-3p added cisplatin sensitivity potentially through regulating PI3K/Akt signaling pathway. Taken together, MiR-27a-3p acted as a tumor suppressor gene in HCC cells, and it could be useful for modulating cisplatin sensitivity in chemotherapy therapy.
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http://dx.doi.org/10.1042/BSR20192007DOI Listing
June 2021

Oral Microbiome Characteristics in Patients With Autoimmune Hepatitis.

Front Cell Infect Microbiol 2021 19;11:656674. Epub 2021 May 19.

Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Autoimmune hepatitis (AIH) is a common cause of liver cirrhosis. To identify the characteristics of the oral microbiome in patients with AIH, we collected 204 saliva samples including 68 AIH patients and 136 healthy controls and performed microbial MiSeq sequencing after screening. All samples were randomly divided into discovery cohorts (46 AIH and 92 HCs) and validation cohorts (22 AIH and 44 HCs). Moreover, we collected samples of 12 AIH patients from Hangzhou for cross-regional validation. We described the oral microbiome characteristics of AIH patients and established a diagnostic model. In the AIH group, the oral microbiome diversity was significantly increased. The microbial communities remarkably differed between the two groups. Seven genera, mainly and , were dominant in the HC group, while 51 genera, and , were enriched in the AIH group. Notably, we found 23 gene functions, including Membrane Transport, Carbohydrate Metabolism, and Glycerolipid metabolism that were dominant in AIH and 31 gene functions that prevailed in HCs. We further investigated the correlation between the oral microbiome and clinical parameters. The optimal 5 microbial markers were figured out through a random forest model, and the distinguishing potential achieved 99.88% between 46 AIH and 92 HCs in the discovery cohort and 100% in the validation cohort. Importantly, the distinguishing potential reached 95.55% in the cross-regional validation cohort. In conclusion, this study is the first to characterize the oral microbiome in AIH patients and to report the successful establishment of a diagnostic model and the cross-regional validation of microbial markers for AIH. Importantly, oral microbiota-targeted biomarkers may be able to serve as powerful and noninvasive diagnostic tools for AIH.
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http://dx.doi.org/10.3389/fcimb.2021.656674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170700PMC
May 2021

Recurrence Plot-Based Approach for Cardiac Arrhythmia Classification Using Inception-ResNet-v2.

Front Physiol 2021 17;12:648950. Epub 2021 May 17.

School of Information Technology and Electrical Engineering, University of Queensland, Brisbane, QLD, Australia.

The present study addresses the cardiac arrhythmia (CA) classification problem using the deep learning (DL)-based method for electrocardiography (ECG) data analysis. Recently, various DL techniques have been utilized to classify arrhythmias, with one typical approach to developing a one-dimensional (1D) convolutional neural network (CNN) model to handle the ECG signals in the time domain. Although the CA classification in the time domain is very prevalent, current methods' performances are still not robust or satisfactory. This study aims to develop a solution for CA classification in two dimensions by introducing the recurrence plot (RP) combined with an Inception-ResNet-v2 network. The proposed method for nine types of CA classification was tested on the 1st China Physiological Signal Challenge 2018 dataset. During implementation, the optimal leads (lead II and lead aVR) were selected, and then 1D ECG segments were transformed into 2D texture images by the RP approach. These RP-based images as input signals were passed into the Inception-ResNet-v2 for CA classification. In the CPSC, Georgia, and the PTB_XL ECG databases of the PhysioNet/Computing in Cardiology Challenge 2020, the RP-based method achieved an average F1-score of 0.8521, 0.8529, and 0.8862, respectively. The results suggested the excellent generalization ability of the proposed method. To further assess the performance of the proposed method, we compared the 2D RP-image-based solution with the published 1D ECG-based works on the same dataset. Also, it was compared with two traditional ECG transform into 2D image methods, including the time waveform of the ECG recordings and time-frequency images based on continuous wavelet transform (CWT). The proposed method achieved the highest average F1-score of 0.844, with only two leads of the 12-lead ECG original data, which outperformed other works. Therefore, the promising results indicate that the 2D RP-based method has a high clinical potential for CA classification using fewer lead ECG signals.
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http://dx.doi.org/10.3389/fphys.2021.648950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165394PMC
May 2021

Lateral flow immunoassay based on gold magnetic nanoparticles for the protein quantitative detection: Prostate-specific antigen.

Anal Biochem 2021 Aug 29;627:114265. Epub 2021 May 29.

College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China. Electronic address:

Point-of-care testing (POCT) demands for rapidly obtaining test results by means of portable analytical instruments and auxiliary reagents at the sampling site. It's important for tumor marker to be recognized and detected in early clinical diagnosis. Many studies focused on producing small portable devices that would allow fast, accurate, and on-site detection. This study aimed to report a magnetic quantitative lateral flow immunoassay (LFIA) system based on poly (acrylic acid) (PAA)-modified gold magnetic nanoparticles (PGMNs) for detecting prostate-specific antigen (PSA) qualitatively and quantitatively. The result was easily achievable with a portable magnetic reader within 15 min. Under optimal conditions, as low as 0.17 ng/mL PSA could be detected. The method was validated using a well-established Solin electrochemiluminescence immunoassay and showed high consistency in detecting 84 serum samples (R = 0.98). The quantitative LFIA based on PGMNs established in this study was proven to be rapid, accurate, sensitive, and inexpensive. As a POCT, it can be potentially developed for the quantitative diagnosis of other disease-related protein biomarkers.
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http://dx.doi.org/10.1016/j.ab.2021.114265DOI Listing
August 2021

Identification of the Core MicroRNAs and Potential Molecular Mechanismsin Sarcoidosis Using Bioinformatics Analysis.

Front Mol Biosci 2021 13;8:644232. Epub 2021 May 13.

Clinical Laboratory, The Affiliated Children Hospital of Xi'an Jiaotong University, Xi'an, China.

Sarcoidosis is a systemic heterogeneous inflammatory disease; however, the etiology and pathogenesis of sarcoidosis are still unknown. Herein, we investigated the core microRNAs and potential molecular mechanisms in sarcoidosis. The DE-miRNAs were diagnosed using the LIMMA software package. DIANA-mirPath was employed to perform pathway and GO enrichment analysis of the DE-miRNAs. PPI networks and miRNA-target gene regulatory networks were used to obtain insight into the actions of DE-miRNAs. Expression of the hub genes along with miRNAs was validated in clinical specimens. Overall, 266 DE-miRNAs were screened. Among these DE-miRNAs, hsa-miR-144, hsa-miR-126, as well as hsa-miR-106a were the upmost upregulated miRNAs; hsa-miR-151-3p, hsa-miR-320d, and hsa-miR-324-3p were the top downregulated miRNAs. , , , , , and were mapped as the most targeted hub genes in the upregulation of miRNAs, and and were the most targeted hub genes in the downregulation of miRNA. and were selected and potentially modulated by hsa-miR-20b, hsa-miR-126, and hsa-miR-106a. In sarcoidosis pathological tissue, hsa-miR-126 was highly expressed, and VEGFA and NR3C1 were overexpressed. In conclusion, our results revealed the dysregulation of hsa-miR-126 and a potential regulatory mechanism for pathogenesis in sarcoidosis.
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http://dx.doi.org/10.3389/fmolb.2021.644232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155597PMC
May 2021

A Novel Graphene-Based Nanomaterial Modified Electrochemical Sensor for the Detection of Cardiac Troponin I.

Front Chem 2021 14;9:680593. Epub 2021 May 14.

Department of Cardiology, Chest Hospital of Henan Provincial, Zhengzhou, China.

Acute myocardial infarction has a high clinical mortality rate. The initial exclusion or diagnosis is important for the timely treatment of patients with acute myocardial infarction. As a marker, cardiac troponin I (cTnI) has a high specificity, high sensitivity to myocardial injury and a long diagnostic window. Therefore, its diagnostic value is better than previous markers of myocardial injury. In this work, we propose a novel aptamer electrochemical sensor. This sensor consists of silver nanoparticles/MoS/reduced graphene oxide. The combination of these three materials can provide a synergistic effect for the stable immobilization of aptamer. Our proposed aptamer electrochemical sensor can detect cTnl with high sensitivity. After optimizing the parameters, the sensor can provide linear detection of cTnl in the range of 0.3 pg/ml to 0.2 ng/ml. In addition, the sensor is resistant to multiple interferents including urea, glucose, myoglobin, dopamine and hemoglobin.
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http://dx.doi.org/10.3389/fchem.2021.680593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162784PMC
May 2021

HDAC6 regulates primordial follicle activation through mTOR signaling pathway.

Cell Death Dis 2021 May 29;12(6):559. Epub 2021 May 29.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, 100193, Beijing, China.

Primordial follicle pool established perinatally is a non-renewable resource which determines the female fecundity in mammals. While the majority of primordial follicles in the primordial follicle pool maintain dormant state, only a few of them are activated into growing follicles in adults in each cycle. Excessive activation of the primordial follicles accelerates follicle pool consumption and leads to premature ovarian failure. Although previous studies including ours have emphasized the importance of keeping the balance between primordial follicle activation and dormancy via molecules within the primordial follicles, such as TGF-β, E-Cadherin, mTOR, and AKT through different mechanisms, the homeostasis regulatory mechanisms of primordial follicle activation remain unclear. Here, we reported that HDAC6 acts as a key negative regulator of mTOR in dormant primordial follicles. In the cytoplasm of both oocytes and granulosa cells of primordial follicles, HDAC6 expressed strong, however in those activated primordial follicles, its expression level is relatively weaker. Inhibition or knockdown of HDAC6 significantly promoted the activation of limited primordial follicles while the size of follicle pool was not affected profoundly in vitro. Importantly, the expression level of mTOR in the follicle and the activity of PI3K in the oocyte of the follicle were simultaneously up-regulated after inhibiting of HDAC6. The up-regulated mTOR leads to not only the growth and differentiation of primordial follicles granulosa cells (pfGCs) into granulosa cells (GCs), but the increased secretion of KITL in these somatic cells. As a result, inhibition of HDAC6 awaked the dormant primordial follicles of mice in vitro. In conclusion, HDAC6 may play an indispensable role in balancing the maintenance and activation of primordial follicles through mTOR signaling in mice. These findings shed new lights on uncovering the epigenetic factors involved physiology of sustaining female reproduction.
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http://dx.doi.org/10.1038/s41419-021-03842-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164630PMC
May 2021

Oocyte meiosis-coupled poly(A) polymerase α phosphorylation and activation trigger maternal mRNA translation in mice.

Nucleic Acids Res 2021 06;49(10):5867-5880

MOE Key Laboratory for Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute, Zhejiang University, Hangzhou 310058, China.

Mammalian oocyte maturation is driven by strictly regulated polyadenylation and translational activation of maternal mRNA stored in the cytoplasm. However, the poly(A) polymerase (PAP) that directly mediates cytoplasmic polyadenylation in mammalian oocytes has not been determined. In this study, we identified PAPα as the elusive enzyme that catalyzes cytoplasmic mRNA polyadenylation implicated in mouse oocyte maturation. PAPα was mainly localized in the germinal vesicle (GV) of fully grown oocytes but was distributed to the ooplasm after GV breakdown. Inhibition of PAPα activity impaired cytoplasmic polyadenylation and translation of maternal transcripts, thus blocking meiotic cell cycle progression. Once an oocyte resumes meiosis, activated CDK1 and ERK1/2 cooperatively mediate the phosphorylation of three serine residues of PAPα, 537, 545 and 558, thereby leading to increased activity. This mechanism is responsible for translational activation of transcripts lacking cytoplasmic polyadenylation elements in their 3'-untranslated region (3'-UTR). In turn, activated PAPα stimulated polyadenylation and translation of the mRNA encoding its own (Papola) through a positive feedback circuit. ERK1/2 promoted Papola mRNA translation in a 3'-UTR polyadenylation signal-dependent manner. Through these mechanisms, PAPα activity and levels were significantly amplified, improving the levels of global mRNA polyadenylation and translation, thus, benefiting meiotic cell cycle progression.
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http://dx.doi.org/10.1093/nar/gkab431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191758PMC
June 2021

A Photoluminescent Lead Bromide Hybrid Perovskite Molecular Ferroelastic Semiconductor with Sequential High- Phase Transitions.

J Phys Chem Lett 2021 Jun 27;12(21):5221-5227. Epub 2021 May 27.

Ordered Matter Science Research Center, Nanchang University, Nanchang 330031, People's Republic of China.

Organic-inorganic hybrid lead halide perovskites have attracted great interest for their use in promising optoelectronic applications. However, reports of photoluminescent perovskite molecular ferroelastic semiconductors with sequential high- phase transitions have been scarce. In this work, a one-dimensional lead bromide hybrid perovskite [-dimethylethanolammonium]PbBr has been synthesized, undergoing high- sequential phase transitions at around 351 and 444 K, higher than those of most previously discovered hybrid perovskite phase transition materials. The specific intermolecular hydrogen bond between cationic molecules provides the greatest contribution to its high by increasing the barrier of molecular motion under the temperature stimuli. The prominent ferroelastic domain evolution is visually observed under orthogonally polarized light. In addition, [-dimethylethanolammonium]PbBr exhibits semiconducting and orange light emission characteristics. This finding opens up an avenue for designing high-performance ferroelastic materials and provides great motivation for discovering new multifunctional materials for the next generation of smart devices.
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http://dx.doi.org/10.1021/acs.jpclett.1c01473DOI Listing
June 2021

Identification of significant genes as prognostic markers and potential tumor suppressors in lung adenocarcinoma via bioinformatical analysis.

BMC Cancer 2021 May 26;21(1):616. Epub 2021 May 26.

Department of Pathology, General Hospital of Western Theater Command, NO.270 Tianhui Road, Rongdu Avenue, Jinniu District, Chengdu, 610083, China.

Background: Lung adenocarcinoma (LAC) is the predominant histologic subtype of lung cancer and has a complicated pathogenesis with high mortality. The purpose of this study was to identify differentially expressed genes (DEGs) with prognostic value and determine their underlying mechanisms.

Methods: Gene expression data of GSE27262 and GSE118370 were acquired from the Gene Expression Omnibus database, enrolling 31 LAC and 31 normal tissues. Common DEGs between LAC and normal tissues were identified using the GEO2R tool and Venn diagram software. Next, the Database for Annotation, Visualization, and Integrated Discovery (DAVID) was used to analyze the Gene Ontology and Kyoto Encyclopedia of Gene and Genome (KEGG) pathways. Then, protein-protein interaction (PPI) network of DEGs was visualized by Cytoscape with Search Tool for the Retrieval of Interacting Genes and central genes were identified via Molecular Complex Detection. Furthermore, the expression and prognostic information of central genes were validated via Gene Expression Profiling Interactive Analysis (GEPIA) and Kaplan-Meier analysis, respectively. Finally, DAVID, real-time PCR and immunohistochemistry were applied to re-analyze the identified genes, which were also further validated in two additional datasets from ArrayExpress database.

Results: First, 189 common DEGs were identified among the two datasets, including 162 downregulated and 27 upregulated genes. Next, Gene Ontology and KEGG pathway analysis of the DEGs were conducted through DAVID. Then, PPI network of DEGs was constructed and 17 downregulated central genes were identified. Furthermore, the 17 downregulated central genes were validated via GEPIA and datasets from ArrayExpress, and 12 of them showed a significantly better prognosis. Finally, six genes were identified significantly enriched in neuroactive ligand-receptor interactions (EDNRB, RXFP1, P2RY1, CALCRL) and Rap1 signaling pathway (TEK, P2RY1, ANGPT1) via DAVID, which were further validated to be weakly expressed in LAC tissues via RNA quantification and immunohistochemistry analysis.

Conclusions: The low expression pattern and relation to prognosis indicated that the six genes were potential tumor suppressor genes in LAC. In conclusion, we identified six significantly downregulated DEGs as prognostic markers and potential tumor suppressor genes in LAC based on integrated bioinformatics methods, which could act as potential molecular markers and therapeutic targets for LAC patients.
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http://dx.doi.org/10.1186/s12885-021-08308-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157630PMC
May 2021

High-resolution magnetic resonance and mass spectrometry imaging of the human larynx.

J Anat 2021 May 25. Epub 2021 May 25.

Department of Entomology, University of Wisconsin-Madison, Madison, WI, USA.

High-resolution, noninvasive and nondestructive imaging of the subepithelial structures of the larynx would enhance microanatomic tissue assessment and clinical decision making; similarly, in situ molecular profiling of laryngeal tissue would enhance biomarker discovery and pathology readout. Towards these goals, we assessed the capabilities of high-resolution magnetic resonance imaging (MRI) and matrix-assisted laser desorption/ionisation-mass spectrometry (MALDI-MS) imaging of rarely reported paediatric and adult cadaveric larynges that contained pathologies. The donors were a 13-month-old male, a 10-year-old female with an infraglottic mucus retention cyst and a 74-year-old female with advanced polypoid degeneration and a mucus retention cyst. MR and molecular imaging data were corroborated using whole-organ histology. Our MR protocols imaged the larynges at 45-117 μm in-plane resolution and capably resolved microanatomic structures that have not been previously reported radiographically-such as the vocal fold superficial lamina propria, vocal ligament and macula flavae; age-related tissue features-such as intramuscular fat deposition and cartilage ossification; and the lesions. Diffusion tensor imaging characterised differences in water diffusivity, primary tissue fibre orientation, and fractional anisotropy between the intrinsic laryngeal muscles, mucosae and lesions. MALDI-MS imaging revealed peptide signatures and putative protein assignments for the polypoid degeneration lesion and the N-glycan constituents of one mucus retention cyst. These imaging approaches have immediate application in experimental research and, with ongoing technology development, potential for future clinical application.
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http://dx.doi.org/10.1111/joa.13451DOI Listing
May 2021

The complete mitogenome and phylogenetic analysis of Indian driftfish, (Scombriformes: Nomeidae).

Mitochondrial DNA B Resour 2021 May 10;6(5):1606-1607. Epub 2021 May 10.

School of Fishery, Zhejiang Ocean University, Zhoushan, China.

The Indian driftfish () is one of the most important commercial fish species in China, Japan and India. The complete mitogenome of was determined in this study. The assembled mitogenome was 16,507 bp and consisted of 13 protein-coding genes, 22 tRNAs, two rRNAs, and a control region. Nucleotide composition of the complete mitogenome was 27.5% A, 28.5% C, 17.5% G, and 26.5% T, with an A + T bias of 53.9%. The maximum-likelihood tree based on 13 protein-coding genes showed that and were the closest to .
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http://dx.doi.org/10.1080/23802359.2021.1914235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118399PMC
May 2021

The Prognostic Value of Non-Predominant Micropapillary Pattern in a Large Cohort of Resected Invasive Lung Adenocarcinoma Measuring ≤3 cm.

Front Oncol 2021 7;11:657506. Epub 2021 May 7.

Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin Lung Cancer Center, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

The aim of this study was to analyze the influence of non-predominant micropapillary pattern in small sized invasive lung adenocarcinoma. A total of 986 lung adenocarcinoma patients with tumor size ≤3 cm were identified and classified according to the IALSC/ATS/ERS classification. Emphasis was placed on the impact of non-predominant micropapillary pattern on disease-free survival (DFS) and overall survival (OS). The relationship between lung adenocarcinoma subtype and lymph node involvement, EGFR mutation and KRAS mutation was also evaluated. A nomogram was developed to predict the probability of 3- and 5-year OS for these patients. The concordance index and calibration plot were used to validate this model. Among all 986 patients, the percentages of lymph node involvement were: 58.1, 50.0, 33.5, 21.4, 21.1, 10.9, 0, and 0% for micropapillary predominant, solid predominant, acinar predominant, papillary predominant, invasive mucinous adenocarcinoma (IMA), lepidic predominant, minimally invasive adenocarcinoma (MIA), adenocarcinoma (AIS), respectively. The frequency of EGFR mutation in the cases of lepidic predominant, acinar predominant, MIA, micropapillary predominant, papillary predominant, solid predominant, IMA, and AIS were 51.1, 45.2, 44.4, 36.8, 29.3, 26.8, 8.3, and 0%, respectively. A non-predominant micropapillary pattern was observed in 344 (38.4%) invasive adenocarcinoma (IAC), and its presence predicted a poorer DFS (median: 56.0 months vs. 66.0 months, 0.001) and OS (median: 61.0 months vs. 70.0 months, 0.001). After propensity score matching, non-predominant micropapillary pattern retained its unfavorable effect on DFS ( = 0.007) and OS ( = 0.001). Multivariate analysis showed that non-predominant micropapillary pattern was identified as an independent prognostic factor for DFS ( = 0.003) and OS (0.001) in IAC. The nomogram showed good calibration and reliable discrimination ability (C-index = 0.775) to evaluated the 3- and 5-year OS. This retrospective analysis of patients with small sized IAC suggests the value of non-predominant micropapillary pattern to predict poor prognosis. A reliable nomogram model was constructed to provide personalized survival predictions.
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http://dx.doi.org/10.3389/fonc.2021.657506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137894PMC
May 2021

Effect of trachea stiffness on tumor distribution in papillary thyroid microcarcinoma.

Oncol Lett 2021 Jul 7;22(1):518. Epub 2021 May 7.

Department of Maxillofacial and Ear Nose and Throat Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300020, P.R. China.

Biomechanical factors play an important role in tumor distribution, epithelial-mesenchymal transition (EMT), invasion and other important processes. Despite fewer reports investigating biomechanical function in papillary thyroid carcinoma (PTC), a large number of PTC cases are located close to the trachea and the majority of advanced cases of PTC have been associated with invasion of the trachea. However, the effect of trachea stiffness on PTC distribution and growth remains unknown. To clarify this issue, two types of PTC cells (TPC-1 and KTC-1) were seeded on a substrate with different stiffness to observe cell proliferation and movement. To identify the effect of trachea stiffness on the thyroid, two thyroid lobes (left and right) were evenly divided into interior (close to the trachea) and lateral (away from the trachea) parts, based on the vertical line between the trachea and thyroid lateral margin with different von Mises stress values. As PTC originates from papillary thyroid microcarcinoma (PTMC) with a maximum diameter of <1 cm, the present study selected PTMC as the study subject to reflect initial PTC distribution in the thyroid. The association between the percentage of PTMC distribution in different parts of the thyroid and von Mises stress values was analyzed. Both PTC cells exhibited stronger proliferation and mobility on the stiff substrate compared with that on the soft substrate. Furthermore, the results of finite element analysis revealed that the von Mises stress values of the interior parts of the trachea were notably higher compared with that in the lateral parts. PTMC distribution in the interior trachea was notably greater compared with that in the lateral section. There was also an observed association between von Mises stress values and PTMC distribution. In addition, the results of RNA-sequencing and reverse transcription-quantitative PCR demonstrated that three biomechanical genes were overexpressed in PTMC located in the interior section compared with that in adjacent normal tissue, and the related signaling pathways were also activated in these tissues. On the whole, these results indicated that trachea stiffness may supply a suitable biomechanical environment for PTMC growth, and the related biomechanical genes may serve as novel targets for PTMC diagnosis and prognostic estimation.
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http://dx.doi.org/10.3892/ol.2021.12779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130054PMC
July 2021

C1orf63 silencing affects breast cancer cell proliferation, apoptosis, and cycle distribution by NF-κB signaling pathway.

Am J Transl Res 2021 15;13(4):2599-2607. Epub 2021 Apr 15.

Department of Thyroid, Breast and Vascular Surgery, Renmin Hospital, Hubei University of Medicine Shiyan, Hubei Province, China.

Objective: To investigate the effect of C1orf63 on breast cancer cell (BCC) proliferation, apoptosis, and cycle distribution and related mechanisms.

Methods: The expression of C1orf63 was interfered with in BCC line MCF and cells were divided into a C1orf63 overexpression group, C1orf63 silence group, blank group, and empty group. The mRNA expression of C1orf63 and the proliferation, apoptosis, and cycle distribution of BCCs were detected. The mRNA expression levels of NF-κB signaling pathway factors (p-IκBα, CyclinD1, CDK4, Bcl-2, and Bax) in each group were also detected.

Results: There was no significant difference between the blank group and empty group in the expression level of C1orf63 mRNA, cell proliferation rate, apoptosis rate, cell distribution rate, or mRNA expression levels of the NF-κB signaling pathway factors (all P>0.05). The expression levels of C1orf63 mRNA in the C1orf63 silenced group were lower than those in the other two groups (P<0.05). The cell proliferation rate, cell distribution in S phase and G2/M phase, and the mRNA expression levels of NF-κB signaling pathway factors (p-IκBα, CyclinD1, CDK4, and Bcl-2) in the C1orf63 silenced group at each time point were lower than those in the other two groups (all P<0.05). The apoptosis rate, cells in G1 phase, and the Bax mRNA expression level in C1orf63 silenced group at each time point were higher than those in the other two groups (all P<0.05).

Conclusion: Down-regulation of C1orf63 acts on the NF-κB signaling pathway to regulate the expression of p-IκBα, CyclinD1, and CDK4, so as to inhibit BCC proliferation, promote cell apoptosis, and block the cell cycle.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129316PMC
April 2021

Autophagy induced by a novel triazol derivative promotes angiogenesis via decreasing interferon-inducible protein 10 level in vascular endothelial cells.

J Cardiovasc Pharmacol 2021 Apr 17. Epub 2021 Apr 17.

School of Biological Science and Technology, University of Jinan, Jinan 250022, China. Shandong Provincial Hospital Affiliated to Shandong University.

Abstract: Autophagy plays an important role in angiogenesis, while the mechanisms of vascular endothelial cell (VEC) autophagy associated with angiogenesis remain unclear. In this study, we identified a novel triazol derivative (JL025) that significantly promoted angiogenesis both in vitro and in vivo. Moreover, JL025 had no effects on cell proliferation but dramatically increased the autophagy level of VEC. The suppression of autophagy inhibited JL025-induced angiogenesis in vitro and in vivo, suggesting that JL025-induced angiogenesis was dependent on the enhanced autophagy. Mechanistic studies indicated that JL025-induced VEC autophagy was related to the Akt/mTOR signaling pathway. Meanwhile, JL025 decreased the anti-angiogenic chemokine interferon-inducible protein 10 (IP10) protein level in HUVECs. Importantly, suppression of autophagy inhibited JL025-induced decrease of IP10 protein level, indicating that autophagy mediated the degradation of IP10. Taken together, our findings provide new insights into the relationship of VEC autophagy with angiogenesis, and JL025 may have a therapeutic potential in related diseases.
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http://dx.doi.org/10.1097/FJC.0000000000001034DOI Listing
April 2021

The Effects of Inactive Platelet-rich Plasma at Different Injection Time on Prefabricated Flap Viability in Rabbits.

Ann Plast Surg 2021 Jun;86(6):701-706

From the Ninth Department of Plastic Surgery, Plastic Surgery Hospital (Institute), Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.

Purpose: To evaluate the effect of inactive form of platelet-rich plasma (PRP) at different injection time on the prefabricated flap viability in rabbits.

Methods: A thoracodorsal artery vascular bundle prefabricated flap was established on the back of 30 healthy male New Zealand white rabbits by 2 phases of operations. A total of 60 sides were randomly divided into the experimental side and the control side. The experimental side was injected with autologous inactive PRP around the thoracodorsal vascular bundle, and the control side was injected with the same amount of normal saline. According to preoperative (group A), intraoperative (group B), and postoperative (group C) injection of PRP, 30 rabbits were divided into 3 groups of 10 each. Histological and immunohistochemical observation, vascular density measurement, and lead oxide angiography were used to compare the differences in the survival of prefabricated flaps between the 3 groups of rabbits injected with inactive PRP at different injection time.

Results: The flap survival ratio, microvessels density, vascular endothelial growth factor and vascular endothelial growth factor receptor 2 expression of PRP injection side were significantly improved compared with the control side. In the comparison of 3 injection time groups, we found that group A and group B could achieve better results, and especially group B had the most significant effect on the revascularization and flap viability.

Conclusion: Autologous inactive PRP can promote the survival of the prefabricated flap, and the intraoperative injection can significantly improve the survival rate of the prefabricated flap. It provides a theoretical guidance for obtaining a larger area of prefabricated axial flap in clinical practice.
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http://dx.doi.org/10.1097/SAP.0000000000002541DOI Listing
June 2021