Publications by authors named "Hua Yang"

1,977 Publications

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Severe deformity in long-term Kaschin-Beck disease.

Authors:
Qin Shao Hua Yang

Rheumatology (Oxford) 2021 Jun 15. Epub 2021 Jun 15.

Department of Radiology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, China.

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http://dx.doi.org/10.1093/rheumatology/keab486DOI Listing
June 2021

Promoting effect of the Maillard reaction products produced during the stir-frying process of on the intestinal absorption of active ingredients in .

Food Sci Biotechnol 2021 May 23;30(5):631-642. Epub 2021 Apr 23.

School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.

This study was designed to evaluate the absorption promoting capacity of Maillard Reaction Products (MRPs) produced during the stir-frying process of on catechin, ferulic acid, quercetin and kaempferol by the ex vivo rat everted gut sac model, in situ single-pass intestinal perfusion model and the whole animal model. Moreover, verapamil, EDTA and mannitol were used for determining the transport mechanism of catechin, ferulic acid, quercetin and kaempferol. The tight junction (TJ) proteins including zonula occudens-1(ZO-1) and claudin-1 were chosen to investigate the promoting mechanism of MRPs by quantitative real-time PCR (qRT-PCR) and western blot analyses. The results showed that the MRPs produced during the stir-frying process of could improve the intestinal absorption of catechin, ferulic acid, quercetin and kaempferol. And the absorption-promoting effect of MRPs was related to chelating effect and the reduced expression of claudin-1 and ZO-1. Our results suggested that MRPs could be promising oral absorption promoters, which might be another processing mechanism of .
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http://dx.doi.org/10.1007/s10068-021-00911-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144268PMC
May 2021

The association of triglyceride and glucose index, and triglyceride to high-density lipoprotein cholesterol ratio with prehypertension and hypertension in normoglycemic subjects: A large cross-sectional population study.

J Clin Hypertens (Greenwich) 2021 Jun 12. Epub 2021 Jun 12.

Tianjin Chest Hospital, Tianjin, China.

Insulin resistance (IR) plays an important role in the development of hypertension. Triglyceride and glucose index (TyG index), and triglyceride to high-density lipoprotein cholesterol ratio (TG/HDL-c) as effective IR surrogate indexes have been verified in numerous studies. Therefore, the authors conducted a large cross-sectional study to explore the association of TyG index and TG/HDL-c with prehypertension and hypertension in the same normoglycemic subjects from Tianjin, China. A total of 32 124 adults were eligible for this study. According to the level of blood pressure, the enrolled individuals were divided into three groups, which were normotension, prehypertension, and hypertension. In multiple logistic regression analysis, there was associated with prehypertension and hypertension when comparing the highest TyG index to the lowest TyG index and corresponding ORs were 1.795 (1.638, 1.968) and 2.439 (2.205, 2.698), respectively. For TG/HDL-c, the corresponding ORs were 1.514 (1.382, 1.658) and 1.934 (1.751, 2.137), respectively. Furthermore, when comparing the fourth quartile to the first quartile of TyG index and TG/HDL-c, respectively, both corresponding ORs of hypertension were higher than prehypertension. Elevated TyG index and TG/HDL-c levels were associated with prehypertension and hypertension in normoglycemic individuals. Moreover, the TyG index was more significant than TG/HDL-c in distinguishing hypertension. They have the potential to become cost-effective monitors in the hierarchical management of prehypertension and hypertension.
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http://dx.doi.org/10.1111/jch.14305DOI Listing
June 2021

Interactive Effects of Lanthanum and Calcium on Cadmium Accumulation in Wheat with Special Reference to Expression Regulated by Calmodulin.

J Agric Food Chem 2021 Jun 8. Epub 2021 Jun 8.

School of Resources and Environmental Science, Wuhan University, Wuhan, Hubei 430079, People's Republic of China.

Lanthanum (La), calcium (Ca), and cadmium (Cd) have similar physical and chemical properties because of their similar ionic radius. Although the interactions between La, Ca, and Cd have been frequently reported in plants, few studies have investigated the interactive effects of La and Ca on the growth and Cd accumulation in plants. Therefore, we investigated the interactive effects of La and Ca on the growth and Cd accumulation in wheat under Cd exposure by a hydroponic experiment. The results indicated that wheat growth was significantly affected by La-Cd and La-Ca interactions. The accumulation of Cd in wheat was significantly affected by La-Ca and La-Cd interactions and La-Ca-Cd interplay. Correlation analysis indicated that Ca deficiency stimulated La to promote wheat growth and mitigate Cd toxicity. Simultaneously, a low Ca supply stimulated La to decrease Cd accumulation in wheat and induce expression. In addition, Cd accumulation in wheat was significantly affected by the W7-La interaction and W7-La-Ca interplay. All of the results suggested that La, Ca, and Cd probably share the same binding sites in calmodulin (TaCaM) and La could affect Cd accumulation in wheat by interacting with TaCaM and then downregulating the expression of .
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http://dx.doi.org/10.1021/acs.jafc.1c00365DOI Listing
June 2021

Involves in the Modulation of Intestinal ANGPTLT4 Expression in Mice Treated by High-Fat Diet.

Front Nutr 2021 19;8:690138. Epub 2021 May 19.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Increasing studies have shown that obesity is the primary cause of cardiovascular diseases, non-alcoholic fatty liver diseases, type 2 diabetes, and a variety of cancers. The dysfunction of gut microbiota was proved to result in obesity. Recent research indicated ANGPTL4 was a key regulator in lipid metabolism and a circulating medium for gut microbiota and fat deposition. The present study was conducted to investigate the alteration of gut microbiota and ANGPTL4 expression in the gastrointestinal tract of mice treated by the high-fat diet. Ten C57BL/6J mice were randomly allocated to two groups and fed with a high-fat diet (HFD) containing 60% fat or a normal-fat diet (Control) containing 10% fat. The segments of ileum and colon were collected for the determination of ANGPTL4 expression by RT-qPCR and immunohistochemical analysis while the ileal and colonic contents were collected for 16S rRNA gene sequencing. The results showed HFD significantly increased mice body weight, epididymal fat weight, perirenal fat weight, liver weight, and the lipid content in the liver ( < 0.05). The relative expression of ANGPTL4 and the ANGPTL4-positive cells in the ileum and colon of mice was significantly increased by HFD treatment. Furthermore, 16S rRNA gene sequencing of the ileal and colonic microbiota suggested that HFD treatment changed the composition of the gut microbiota. The ratio of Firmicutes to Bacteroidetes and the abundance of was significantly higher in the HFD group than in the Control group while the abundance of , and was significantly decreased. Interestingly, the abundance of was positively correlated with the expression of ANGPTL4. These findings provide a theoretical foundation for the development of strategies to control the obesity and related diseases by the regulation of ANGPTL4 and gut microbiota.
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http://dx.doi.org/10.3389/fnut.2021.690138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171929PMC
May 2021

A Novel Role of AR in the Maintenance of Intestinal Barrier Function of Enteric Glia from Hypoxia-Induced Injury by Combining with mGluR5.

Front Pharmacol 2021 10;12:633403. Epub 2021 May 10.

Department of General Surgery, Xinqiao Hospital, Army Medical University, Chongqing, China.

During acute intestinal ischemia reperfusion (IR) injury, the intestinal epithelial barrier (IEB) function is often disrupted. Enteric glial cells (EGCs) play an important role in maintaining the integrity of IEB functions. However, how EGCs regulate IEB function under IR stimulation is unknown. The present study reveals that the adenosine A receptor (AR) is important for mediating the barrier-modulating roles of EGCs. AR knockout (KO) experiments revealed more serious intestinal injury in AR KO mice than in WT mice after IR stimulation. Moreover, AR expression was significantly increased in WT mice when challenged by IR. To further investigate the role of AR in IEB, we established an EGC-Caco-2 co-culture system. Hypoxia stimulation was used to mimic the process of IR. Treating EGCs with the CGS21680 AR agonist attenuated hypoxia-induced intestinal epithelium damage through up-regulating ZO-1 and occludin expression in cocultured Caco-2 monolayers. Furthermore, we showed that AR and metabotropic glutamate receptor 5 (mGluR5) combine to activate the PKCα-dependent pathway in conditions of hypoxia. This study shows, for the first time, that hypoxia induces AR-mGluR5 interaction in EGCs to protect IEB function via the PKCα pathway.
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http://dx.doi.org/10.3389/fphar.2021.633403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173626PMC
May 2021

Sequence of the supernumerary B chromosome of maize provides insight into its drive mechanism and evolution.

Proc Natl Acad Sci U S A 2021 Jun;118(23)

Department of Electrical Engineering and Computer Science, University of Missouri, Columbia, MO 65211.

B chromosomes are enigmatic elements in thousands of plant and animal genomes that persist in populations despite being nonessential. They circumvent the laws of Mendelian inheritance but the molecular mechanisms underlying this behavior remain unknown. Here we present the sequence, annotation, and analysis of the maize B chromosome providing insight into its drive mechanism. The sequence assembly reveals detailed locations of the elements involved with the and functions of its drive mechanism, consisting of nondisjunction at the second pollen mitosis and preferential fertilization of the egg by the B-containing sperm. We identified 758 protein-coding genes in 125.9 Mb of B chromosome sequence, of which at least 88 are expressed. Our results demonstrate that transposable elements in the B chromosome are shared with the standard A chromosome set but multiple lines of evidence fail to detect a syntenic genic region in the A chromosomes, suggesting a distant origin. The current gene content is a result of continuous transfer from the A chromosomal complement over an extended evolutionary time with subsequent degradation but with selection for maintenance of this nonvital chromosome.
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http://dx.doi.org/10.1073/pnas.2104254118DOI Listing
June 2021

Relationship between free fatty acids, body mass index and depressive symptoms in patients with chronic heart failure.

Nurs Open 2021 Jun 3. Epub 2021 Jun 3.

Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Aim: To explore the prevalence of depressive symptoms among patients with chronic heart failure (CHF) and analyse the relationship between free fatty acids (FFAs), body mass index (BMI) and depressive symptoms among patients with CHF.

Design: A cross-sectional study.

Methods: Questionnaires were distributed to 200 patients with CHF in the department of Cardiology at a tertiary first-class hospital in Shandong province. A total of 195 hospitalized patients completed the survey. Data collected from February 2017-November 2017 were analysed by using Pearson's correlation and multiple linear regression. The depressive status of the patients was assessed by the Zung Self-rating Depression Scale (SDS), and the relationships between FFA, BMI and depressive symptoms were analysed using SPSS 20.0.

Results: The results showed that 71.28% CHF patients have depressive symptoms. The serum FFA level was significantly higher in CHF patients with depressive symptoms than those without depressive symptoms (p = .003), and FFA level was significantly positively correlated with the SDS score (r = .242, p = .001). The differences in SDS scores were statistically significant between two different BMI groups (p < .01), and BMI was negatively correlated with the SDS score (r = -.139, p = .040). Regression analysis showed that high FFAs (β = 0.184, p = .009) and low BMI (β = -0.116, p = .049) were risk factors for depressive symptoms in CHF patients. Gender, age, FFA and BMI were associated with depressive symptoms, accounting for 7.1% of the variance in depressive symptoms.

Conclusions: The prevalence of depressive symptoms in CHF patients is very high, and high FFAs and low BMI are risk factors for depressive symptoms. Targeted interventions to strengthen nutritional support in CHF patients may be benefit to improve depression-related outcomes.
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http://dx.doi.org/10.1002/nop2.877DOI Listing
June 2021

Photocatalytic Cyclization/Defluorination Domino Sequence to Access 3-Fluoro-1,5-dihydro-2-pyrrol-2-one Scaffold.

Org Lett 2021 Jun 1;23(12):4754-4758. Epub 2021 Jun 1.

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China.

We herein report an unprecedented photoinduced cyclization/defluorination domino process of -allylbromodifluoroacetamide with cyclic secondary amines. Consequently, a wide array of valuable 3-fluoro-1,5-dihydro-2-pyrrol-2-ones were facilely prepared from readily available starting materials under mild conditions. Preliminary mechanistic investigations suggest that a radical chain propagation and amine-promoted defluorination pathway are presumably involved in this transformation.
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http://dx.doi.org/10.1021/acs.orglett.1c01477DOI Listing
June 2021

Whole-genome resequencing provides insights into the population structure and domestication signatures of ducks in eastern China.

BMC Genomics 2021 May 31;22(1):401. Epub 2021 May 31.

Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Background: Duck is an ancient domesticated animal with high economic value, used for its meat, eggs, and feathers. However, the origin of indigenous Chinese ducks remains elusive. To address this question, we performed whole-genome resequencing to first explore the genetic relationship among variants of these domestic ducks with their potential wild ancestors in eastern China, as well as understand how the their genomes were shaped by different natural and artificial selective pressures.

Results: Here, we report the resequencing of 60 ducks from Chinese spot-billed ducks (Anas zonorhyncha), mallards (Anas platyrhnchos), Fenghua ducks, Shaoxing ducks, Shanma ducks and Cherry Valley Pekin ducks of eastern China (ten from each population) at an average effective sequencing depth of ~ 6× per individual. The results of population and demographic analysis revealed a deep phylogenetic split between wild (Chinese spot-billed ducks and mallards) and domestic ducks. By applying selective sweep analysis, we identified that several candidate genes, important pathways and GO categories associated with artificial selection were functionally related to cellular adhesion, type 2 diabetes, lipid metabolism, the cell cycle, liver cell proliferation, and muscle functioning in domestic ducks.

Conclusion: Genetic structure analysis showed a close genetic relationship of Chinese spot-billed ducks and mallards, which supported that Chinese spot-billed ducks contributed to the breeding of domestic ducks. During the long history of artificial selection, domestic ducks have developed a complex biological adaptation to captivity.
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http://dx.doi.org/10.1186/s12864-021-07710-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165772PMC
May 2021

Nanosecond pulsed electric fields impair viability and mucin expression in mucinous colorectal carcinoma cell.

Bioelectrochemistry 2021 May 20;141:107844. Epub 2021 May 20.

School of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.

Nanosecond pulsed electric fields (nsPEFs) are a non-thermal technology that can induce a myriad of biological responses and changes in cellular physiology. nsPEFs have gained significant attention as a novel cancer therapy. However, studies investigating the application of nsPEF in mucinous carcinomas are scarce. In this study, we explored several biological responses in two mucinous colorectal adenocarcinoma cell lines, LS 174T and HT-29, to nsPEF treatment. We determined the overall cell survival and viability rates following nsPEF treatment using CCK-8 and colony formation assays. We measured the intracellular effects of nsPEF treatment by analyzing cell cycle distribution, cell apoptosis and mitochondrial potential. We also analyzed mucin production at both mRNA and protein levels. Our results showed that nsPEF treatment significantly reduced mucinous cell viability in a dose-dependent manner. nsPEF treatment increased cell cycles arrest at G/G while the proportion of G/M cells gradually decreased. Cell apoptosis increased following nsPEF treatment with a clear loss in mitochondrial membrane potential. Furthermore, the protein expression of functional mucin family members decreased after nsPEF treatment. In conclusion, nsPEF treatment reduced MCRC cell viability, cell proliferation, and mucin protein production while promoted apoptosis. Our work is a pilot study that projects some insights into the potential clinical applications of nsPEFs in treating mucinous colorectal carcinoma.
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http://dx.doi.org/10.1016/j.bioelechem.2021.107844DOI Listing
May 2021

CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis.

Invest Ophthalmol Vis Sci 2021 May;62(6):28

Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao, China.

Purpose: C-type lectin-like receptor-1 (CLEC-1) is a member of the Dectin-1 cluster of pattern recognition receptors (PRRs). It is involved in host immunity, has immunoregulatory function, and supports allograft tolerance. Our study aimed to describe the role of CLEC-1 in response to fungal keratitis, in situ, in vivo, and in vitro.

Methods: Quantitative polymerase chain reaction (qRT-PCR) and immunofluorescence were used to detect the expression of CLEC-1 in corneas of patients with Aspergillus fumigatus (A. fumigatus) keratitis. In vitro and in vivo experiments were designed in THP-1 macrophages and C57BL/6 mouse models, respectively. The expression of CLEC-1 in corneas of mice model was determined by qRT-PCR, Western blot, and immunofluorescence. CLEC-1 overexpression in mouse corneas was achieved by intrastromal injection of adeno-associated virus (AAV) vectors. Disease response was evaluated by slit-lamp photography, clinical score, and colony forming unit (CFU). Bioluminescence imaging system image acquisition, myeloperoxidase (MPO) assays, immunofluorescence staining, qRT-PCR, and Western blot were used to investigate the role of CLEC-1. To further define the role of CLEC-1, we used lentivirus vectors to overexpress CLEC-1 or/and Dectin-1 in THP-1 macrophages.

Results: The expression of CLEC-1 was increased in corneas of patients with A. fumigatus keratitis. In corneas of mice from the A. fumigatus keratitis model, the expression of CLEC-1 was decreased in the acute inflammatory stage and increased during convalescence. Following Natamycin treatment, CLEC-1 was upregulated in A. fumigatus keratitis mice. Compared with normal C57BL/6 mice, overexpression of CLEC-1 converted the characteristic susceptible response to resistance, as demonstrated by slit-lamp photography and clinical score. In vivo studies revealed decreased MPO levels and neutrophils recruitment and higher fungal load after the upregulation of CLEC-1. Compared with control corneas, CLEC-1 overexpression impaired corneal pro-inflammatory cytokine IL-1β production.

Conclusions: These findings demonstrate that CLEC-1 may act as a negative regulator of Dectin-1 induced host inflammatory response via suppressing neutrophils recruitment and production of pro-inflammatory cytokine IL-1β production in response to A. fumigatus keratitis.
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http://dx.doi.org/10.1167/iovs.62.6.28DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164365PMC
May 2021

Similar Antimicrobial Resistance of Strains Isolated from Retail Chickens and Poultry Farms.

Foodborne Pathog Dis 2021 May 25. Epub 2021 May 25.

Institute of Agro-Product Safety and Nutrition, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Antimicrobial resistance (AMR) is a major public health challenge and spreads through humans, animals, and the environment. Many reports show that AMR genes (ARGs) or phenotypes can be transferred from food animals to humans. However, the level and correlation of AMR in different nodes of the poultry meat supply chain are still poorly understood. Herein, 225 isolates were recovered from chilled chicken samples from markets (123) and chicken fecal samples from farms (102) in Zhejiang Province, China. The dominant sequence types (STs) were ST155 (8.89%), ST48 (7.56%), and ST10 (7.11%), which are common in chicken and fecal samples. Antimicrobial susceptibility testing (AST) analysis showed that the isolates from fecal samples and retail chickens were resistant to ampicillin (61.77% and 63.42%, respectively) and trimethoprim (56.87% and 52.85%). Moreover, 36.59% of the isolates from chilled chickens and 39.22% of the isolates from fecal samples were resistant to three or more antimicrobial agents. A total of 59 ARGs were identified in sequenced genomes, including the gene involved in colistin resistance. The from farms and markets could be clustered in the same branch according to core single nucleotide polymorphisms. In addition, toxin genes and were also predicted in 86.5% (32/37) and 13.5% (5/37) of the above genomes, respectively. Taken together, these findings demonstrated that isolates from markets and farms showed similar AMR patterns, suggesting that strains in markets may originate from farms.
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http://dx.doi.org/10.1089/fpd.2021.0019DOI Listing
May 2021

Effects of donepezil on the amplitude of low-frequency fluctuations in the brain of patients with Alzheimer's disease: evidence from resting-state functional magnetic resonance imaging.

Neuroreport 2021 May 20. Epub 2021 May 20.

Department of Geriatrics Department of Pharmacy, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China.

Objectives: To monitor the effects of donepezil on spontaneous neuronal activity (SNA), and the mechanisms underlying these effects in patients with mild-to-moderate Alzheimer's disease, using the amplitude of low-frequency fluctuations (ALFFs), a metric of resting-state functional MRI (rs-fMRI).

Methods: Eleven patients with Alzheimer's disease were treated with donepezil for 6 months. Before and after treatment, the Mini-Mental State Examination (MMSE), Alzheimer's Disease Assessment Scale-Cognitive Subscale (ADAS-cog), Clinical Dementia Rating (CDR), Neuropsychiatric Inventory and Activities of Daily Living scores, along with rs-fMRI of patients were assessed. Eleven age-, sex-, and education-matched controls underwent MMSE and CDR assessments and rs-fMRI at enrollment. The ALFFs of the whole brain were obtained and compared between the groups.

Results: Following donepezil treatment, MMSE scores increased (P = 0.043) and ADAS-cog scores decreased (P = 0.010). Regarding SNA post-treatment, ALFF increased significantly in the right triangular part of the inferior frontal gyrus (IFGtriang; P = 0.030; d = -0.595) and the right orbital part of the inferior frontal gyrus (P = 0.044; d = -0.628) and decreased significantly in the left medial orbital part of the superior frontal gyrus (P = 0.039; d = 0.606) and the right gyrus rectus (P = 0.010; d = 0.609). Furthermore, the changes in ADAS-cog scores from before to after treatment were positively correlated with the changes in ALFF in the right IFGtriang (r = 0.645; P = 0.032).

Conclusions: Donepezil improved SNA in the frontal lobe of patients with Alzheimer's disease. Therefore, ALFF was demonstrated to be a potential tool for assessing the effectiveness of Alzheimer's disease treatment.
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http://dx.doi.org/10.1097/WNR.0000000000001659DOI Listing
May 2021

Identification of key genes in calcific aortic valve disease via weighted gene co-expression network analysis.

BMC Med Genomics 2021 May 21;14(1):135. Epub 2021 May 21.

Department of Cardiology, Liyang People's Hospital, Liyang, 213300, China.

Background: Calcific aortic valve disease (CAVD) is the most common subclass of valve heart disease in the elderly population and a primary cause of aortic valve stenosis. However, the underlying mechanisms remain unclear.

Methods: The gene expression profiles of GSE83453, GSE51472, and GSE12644 were analyzed by 'limma' and 'weighted gene co-expression network analysis (WGCNA)' package in R to identify differentially expressed genes (DEGs) and key modules associated with CAVD, respectively. Then, enrichment analysis was performed based on Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, DisGeNET, and TRRUST database. Protein-protein interaction network was constructed using the overlapped genes of DEGs and key modules, and we identified the top 5 hub genes by mixed character calculation.

Results: We identified the blue and yellow modules as the key modules. Enrichment analysis showed that leukocyte migration, extracellular matrix, and extracellular matrix structural constituent were significantly enriched. SPP1, TNC, SCG2, FAM20A, and CD52 were identified as hub genes, and their expression levels in calcified or normal aortic valve samples were illustrated, respectively.

Conclusions: This study suggested that SPP1, TNC, SCG2, FAM20A, and CD52 might be hub genes associated with CAVD. Further studies are required to elucidate the underlying mechanisms and provide potential therapeutic targets.
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http://dx.doi.org/10.1186/s12920-021-00989-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138987PMC
May 2021

Impact of COL6A4P2 gene polymorphisms on the risk of lung cancer: A case-control study.

PLoS One 2021 21;16(5):e0252082. Epub 2021 May 21.

Key Laboratory of Molecular Mechanism and Intervention Research for Plateau Diseases of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, China.

Lung cancer (LC) is a malignant tumor that poses the greatest threat to human health and life. Most studies suggested that the occurrence of LC is associated with environmental and genetic factors. We aimed to explore the association between COL6A4P2 single nucleotide polymorphisms (SNPs) and CHD risk in the Chinese Southern Han population. Based on the 'case-control' experimental design (510 cases and 495 controls), we conducted an association study between five candidate COL6A4P2 SNPs and the corresponding LC risk. Odds ratio (OR) and 95% confidence intervals (CIs) were calculated by logistic regression to analyze the LC susceptibility under different genetic models. The results showed that COL6A4P2 rs34445363 was significantly associated with LC risk under alleles model (OR = 1.26, 95%CI: 1.01-1.58, p = 0.038). In addition, rs34445363 was also significantly associated with LC risk under the log-additive model (OR = 1.26, 95%CI: 1.01-1.58, p = 0.041). The results of subgroup analysis showed that rs34445363 (OR = 1.42, 95%CI: 1.03-1.95, p = 0.033) and rs61733464 (OR = 0.72, 95%CI: 0.52-0.99, p = 0.048) were both significantly associated with LC risk in the log-additive model among participants who were ≤ 61 years old. We also found that the variation of rs34445363 (GA vs. GG, OR = 1.73, 95%CI: 1.04-2.86, p = 0.034) and rs77941834 (TA vs. TT, OR = 1.88, 95%CI: 1.06-3.34, p = 0.032) were associated with LC risk in the codominant model among female participants. Our study is the first to find that COL6A4P2 gene polymorphism is associated with LC risk in the Chinese Han population. Our study provides a basic reference for individualized LC prevention.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252082PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139505PMC
May 2021

Recent progress in the nitration of arenes and alkenes.

Org Biomol Chem 2021 Jun;19(22):4835-4851

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China.

Nitro compounds are a predominant class of synthetic intermediates and building blocks for the preparation of a wide range of nitrogen-containing compounds in the chemical industry. As such, impressive progress has been currently made in the nitration of aromatics and olefins with excellent functional group tolerance and site-selectivity. In this mini review, we intend to highlight the regiospecific nitration of arenes and alkenes in various reaction systems. The involved mechanisms are discussed as well.
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http://dx.doi.org/10.1039/d1ob00384dDOI Listing
June 2021

A validated UHPLC-MS/MS method for pharmacokinetic and brain distribution studies of twenty constituents in rat after oral administration of Jia-Wei-Qi-Fu-Yin.

J Pharm Biomed Anal 2021 Aug 13;202:114140. Epub 2021 May 13.

State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, China. Electronic address:

A rapid ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UHPLC-QqQ MS/MS) approach with high sensitivity and selectivity was developed for the quantification of twenty compounds, including 9 saponins, 8 flavonoids, 2 oligosaccharide esters and 1 phenolic acid, in rat plasma and brain, which was administrated intragastrically with Jia-Wei-Qi-Fu-Yin (JWQFY), Mass spectrometric detection was operated under multiple reaction monitoring (MRM) mode. All calibration curves possessed good linearity with correlation coefficients ( r) higher than 0.9916 in their respective linear ranges. For intra- and inter-day precision, all the relative standard deviations (RSDs) at different levels were less than 14.68 %. Based on the UHPLC-QqQ MS/MS quantitative results, pharmacokinetic study and brain distribution of multiple components in JWQFY was then successfully performed. As a result, constituents with discrepancy structures showed diverse pharmacokinetic and distribution characteristics. For instance, ferulic acid (phenolic acid), 3, 6'-disinapoyl sucrose and tenuifoliside A (oligosaccharide esters) showed short T (< 10 min), whereas the T of ginsenosides Rb, Rb and Rc (ppd-type terpenoid saponins) were much longer (> 4 h). Besides, ferulic acid, epimedin C, icariin, glycyrrhizin, ginsenoside Rb and ginsenoside Rg were considered as the potential effective ingredients of JWQFY because of their relatively high exposure to blood and brain. Our study would provide relevant information for discovery of pharmacodynamic ingredients, as well as further action mechanisms investigations of JWQFY.
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http://dx.doi.org/10.1016/j.jpba.2021.114140DOI Listing
August 2021

Colorectal cancer in pregnancy: a case report and literature review.

J Gastrointest Oncol 2021 Apr;12(2):885-891

Department of Gynecology and Obstetrics, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Colorectal cancer (CRC) in pregnancy is rare. The clinical manifestations of CRC during pregnancy are not specific, and diagnosis and treatment pose a significant challenge. Patients are often found to have advanced tumors, and have a poor prognosis. In this case study, the patient was 36 years old, and had no obvious clinical manifestations in the first and second trimesters. Since gestational week 38, she experienced left upper abdominal pain and constipation, with no nausea or vomiting. Imaging examinations revealed malignant tumors of the sigmoid colon (colon cancer was highly suspected), multiple liver metastases, omental metastases, and multiple swollen lymph nodes in the abdominal cavity. After discussion, the patient received lower cesarean section. A large amount of hematochezia with a volume of approximately 1,000 mL occurred 8 days after the operation. In the emergency department, superior and inferior mesenteric angiography was performed, and the inferior mesenteric artery was continuously pumped with pituitrin to stop bleeding. After the condition was stabilized, she underwent surgery for radical sigmoid resection and colon single-cavity fistula. During the operation, light bloody ascites were observed inside the abdominal pelvis, approximately 500 mL, with multiple touchable stiff metastatic nodules in the liver. At the side of the sigmoid mesocolon, a giant tumor of approximately 12 cm diameter was found, continued by the intestinal wall, and the sigmoid mesocolon was almost occupied by the tumor. The mesentery was hard to recognize, with possible movement of the tumor, and no sign of infiltration into the abdominal pelvic wall or adnexa. The surgical pathological stage was Dukes' stage D, which was an advanced tumor. Postoperative radiotherapy and chemotherapy were not performed, and the patient died of systemic multiple organ failure 32 days after colon cancer surgery (postpartum day 43). So for patients with digestive system symptoms during pregnancy, CRC should be considered in differential diagnosis. Auxiliary examinations should be actively carried out to strive early diagnosis and treatment to improve patient prognosis.
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http://dx.doi.org/10.21037/jgo-21-31DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107627PMC
April 2021

Sensing of mycobacterial arabinogalactan by galectin-9 exacerbates mycobacterial infection.

EMBO Rep 2021 May 13:e51678. Epub 2021 May 13.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Mycobacterial arabinogalactan (AG) is an essential cell wall component of mycobacteria and a frequent structural and bio-synthetical target for anti-tuberculosis (TB) drug development. Here, we report that mycobacterial AG is recognized by galectin-9 and exacerbates mycobacterial infection. Administration of AG-specific aptamers inhibits cellular infiltration caused by Mycobacterium tuberculosis (Mtb) or Mycobacterium bovis BCG, and moderately increases survival of Mtb-infected mice or Mycobacterium marinum-infected zebrafish. AG interacts with carbohydrate recognition domain (CRD) 2 of galectin-9 with high affinity, and galectin-9 associates with transforming growth factor β-activated kinase 1 (TAK1) via CRD2 to trigger subsequent activation of extracellular signal-regulated kinase (ERK) as well as induction of the expression of matrix metalloproteinases (MMPs). Moreover, deletion of galectin-9 or inhibition of MMPs blocks AG-induced pathological impairments in the lung, and the AG-galectin-9 axis aggravates the process of Mtb infection in mice. These results demonstrate that AG is an important virulence factor of mycobacteria and galectin-9 is a novel receptor for Mtb and other mycobacteria, paving the way for the development of novel effective TB immune modulators.
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http://dx.doi.org/10.15252/embr.202051678DOI Listing
May 2021

Global alternative splicing landscape of skeletal muscle atrophy induced by hindlimb unloading.

Ann Transl Med 2021 Apr;9(8):643

Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, China.

Background: Long-term exposure to microgravity will cause skeletal muscle atrophy, which can cause serious harm to astronauts in space travel. Therefore, it is important to explore skeletal muscle atrophy's molecular mechanism for its prevention and treatment. However, as an important regulatory approach of skeletal muscle physiology, the role of alternative splicing in skeletal muscle atrophy, especially skeletal muscle atrophy caused by disuse, is unclear.

Methods: We established a rat hindlimb unloading model and performed RNA sequencing on soleus muscle, which was seriously atrophied during unloading. Several bioinformatics methods were used to identify alternative splicing events and determine their gene functions.

Results: Many alternative splicing events were found to occur at different time points (12 h, 24 h, 36 h, 3 days, and 7 days) after hindlimb unloading. These differential alternative splicing events mainly occurred in the gene's coding domain sequence region, and 59% of the alternative splicing events caused open reading frameshift. Bioinformatics analysis results showed that genes with different alternative splicing events were enriched in multiple pathways related to muscle atrophy, including the insulin signaling pathway, endocytosis, mitophagy, and ubiquitin-proteasome pathway. Moreover, alternative splicing of several deubiquitinase genes persisted during skeletal muscle atrophy induced by unloading. Additionally, we identified 10 differentially expressed RNA binding proteins during skeletal muscle atrophy induced by unloading, mainly containing Xpo4, Eif4e2, P4ha1, Lrrfip1, Zc3h14, Emg1, Hnrnp h1, Mbnl2, RBfox1, and Mbnl1. Hnrnp h1 and Mbnl2 were significantly downregulated, and RBfox1 and Mbnl1 were significantly upregulated during skeletal muscle atrophy caused by unloading.

Conclusions: To the best of our knowledge, the present study is the first to propose alternative splicing alterations related to disuse-induced muscle atrophy, emphasizing that alternative splicing is a new focus of attention in the occurrence of muscle atrophy.
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http://dx.doi.org/10.21037/atm-20-5388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106077PMC
April 2021

Coexistence of and in Campylobacter.

mSphere 2021 05 12;6(3). Epub 2021 May 12.

Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory of Food Quality and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China

Previous studies indicated that has developed several mechanisms that confer resistance to florfenicol, which is used in food animal production. This study describes the coexistence of and in and isolates from pigs and poultry. Moreover, whole-genome sequencing data showed that the two genes are located in various multidrug resistance genomic islands within different regions of the genomes. The emergence of and may support the spread of florfenicol-resistant strains of animal origin. Florfenicol is widely used for the treatment of respiratory infections and as a feed additive in food animal production. As a foodborne pathogen, is constantly exposed to florfenicol, and resistance to this antimicrobial agent has increased in recent years. Previous studies indicated that has developed several mechanisms that confer resistance to florfenicol. This study describes for the first time the coexistence of the florfenicol exporter FexA and the ribosomal protective protein OptrA in isolated from pigs. The two genes were located in various multidrug resistance genomic islands within different regions of the genomes. Although phenicols are not commonly used for the treatment of infections, the extensive use of florfenicol in food animals may play a role in the coselection of multidrug resistance genomic island (MDRGI)-carrying isolates which also exhibited resistance to critically important antimicrobial agents (macrolides, aminoglycosides, and tetracyclines) commonly used for the treatment of human campylobacteriosis.
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http://dx.doi.org/10.1128/mSphere.00125-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125047PMC
May 2021

Serum MicroRNA Transcriptomics and Acute Rejection or Recurrent Hepatitis C Virus in Human Liver Allograft Recipients: A Pilot Study.

Transplantation 2021 May 11. Epub 2021 May 11.

Division of Nephrology and Hypertension, Joan and Sanford I. Weill Department of Medicine and Department of Transplantation Medicine, New York Presbyterian-Weill Cornell Medicine, New York, NY. Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY. Department of Psychiatry and Behavioral Science, Stony Brook University, Stony Brook, NY. Division of Biostatistics, Department of Public Health Sciences, University of California at Davis, Davis, CA. Rho Federal Systems, Chapel Hill, NC. Division of Transplantation Pathology, Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA.

Background: Acute rejection (AR) and recurrent HCV (R-HCV) are significant complications in liver allograft recipients. Noninvasive diagnosis of intragraft pathologies may improve their management.

Methods: We performed small RNA sequencing and miRNA microarray profiling of RNA from sera matched to liver allograft biopsies from patients with nonimmune, nonviral (NINV) native liver disease. Absolute levels of informative miRNAs in 91 sera matched to 91 liver allograft biopsies were quantified using customized RT-qPCR assays: 30 biopsy-matched sera from 26 unique NINV patients and 61 biopsy-matched sera from 41 unique R-HCV patients. The association between biopsy diagnosis and miRNA abundance was analyzed by logistic regression and calculating the area under the receiver operating characteristic curve.

Results: Nine miRNAs- miR-22, miR-34a, miR-122, miR-148a, miR-192, miR-193b, miR-194, miR-210 and miR-885-5p- were identified by both sRNA-seq and TLDA to be associated with NINV-AR. Logistic regression analysis of absolute levels of miRNAs and goodness-of-fit of predictors identified a linear combination of miR-34a + miR-210 (P<0.0001) as the best statistical model and miR-122 + miR-210 (P<0.0001) as the best model that included miR-122. A different linear combination of miR-34a + miR-210 (P<0.0001) was the best model for discriminating NINV-AR from R-HCV with intragraft inflammation, and miR-34a + miR-122 (P<0.0001) was the best model for discriminating NINV-AR from R-HCV with intragraft fibrosis.

Conclusions: Circulating levels of miRNAs, quantified using customized RT-qPCR assays, may offer a rapid and noninvasive means of diagnosing AR in human liver allografts and for discriminating AR from intragraft inflammation or fibrosis due to recurrent HCV.Supplemental Visual Abstract; http://links.lww.com/TP/C231.
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http://dx.doi.org/10.1097/TP.0000000000003815DOI Listing
May 2021

The role of positive nerve root sedimentation sign in the treatment of patients undergoing lumbar disc herniation.

Br J Neurosurg 2021 May 12:1-6. Epub 2021 May 12.

Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, PR China.

Background: To investigate the efficacy of percutaneous transforaminal endoscopic discectomy (PTED) in the treatment of patients who were diagnosed with lumbar disc herniation (LDH) with positive nerve root sedimentation sign (NRSS).

Methods: A total of 86 patients who underwent LDH were recruited and divided into NRSS-positive group ( = 49) and NRSS-negative group ( = 37). The visual analog scale (VAS), Japanese Orthopaedic Association (JOA), and Oswestry disability index (ODI) were used to evaluate the low back pain and functional recovery and were compared between the two groups.

Results: There were no significant differences in the demographic parameters between the two groups. The average area of the dural sac compression in the NRSS-positive group was significantly higher than that in the NRSS-negative group. Patients with a positive NRSS showed a better low back pain relief than those with a negative NRSS at 1 week and 1 month after surgery. The JOA and ODI in the NRSS-positive group were better than those in the NRSS-negative group at 3 months and 6 months postoperatively. The apparent efficiency of JOA and the excellent and good rate of ODI in the negative group was lower than that in the positive group at 6 months after surgery.

Conclusions: Patients undergoing LDH with a positive NRSS showed better pain relief and functional recovery than those with a negative NRSS. The present study suggested that NRSS might be a valuable sign and associated with better clinical outcomes in patients undergoing LDH with the treatment of PTED.
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http://dx.doi.org/10.1080/02688697.2021.1923652DOI Listing
May 2021

Metal enhanced chemiluminescence nanosensor for ultrasensitive bioassay based on silver nanoparticles modified functional DNA dendrimer.

Anal Chim Acta 2021 Jun 21;1165:338541. Epub 2021 Apr 21.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, PR China. Electronic address:

A novel metal enhanced chemiluminescence (MEC) nanosensor was developed for ultrasensitive biosensing and imaging, based on functional DNA dendrimer (FDD), proximity-dependent DNAzyme and silver nanoparticles (AgNPs). The FDD containing two split G-quadruplex structures was prepared through an enzyme-free and step-by-step assembly strategy, and then reacted with AgNPs and hemin molecules to form the FDD/hemin/AgNPs facilely. Such a MEC nanosensor consisted of three modules: FDD (scaffold), the generated G-quadruplex/hemin DNAzyme (signal reporter) and AgNPs (chemiluminescence enhancer). The MEC effect was achieved by controlling the length of DNA sequences between AgNPs on the periphery of FDD and DNAzymes inside it. Such nanosensor exhibited 9-fold amplification and another 6.4-fold metal enhancement in chemiluminescence intensity, which can be easily applied into trace detection of multiple protein markers using a disposable protein immunoarray. The FDD/hemin/AgNPs-based multiplex MEC imaging assay showed wide linear ranges over 5 orders of magnitude and detection limits down to 5× 10 ng L and 1.8 × 10 U mL for cardiac troponin T and carcinoma antigen 125, demonstrating a promising potential in application to protein analysis and clinical diagnosis. Moreover, the MEC nanosensor can be effectively delivered into cells with excellent biocompatibility and outstanding stability, offering a new tool for detection of intracellular targets and suggesting wide applications in bioassay.
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http://dx.doi.org/10.1016/j.aca.2021.338541DOI Listing
June 2021

Targeted resequencing showing novel common and rare genetic variants increases the risk of asthma in the Chinese Han population.

J Clin Lab Anal 2021 Jun 9;35(6):e23813. Epub 2021 May 9.

Division of Cardiology, Departments of Internal Medicine and Genetic Diagnosis Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Although studies have identified hundreds of genetic variants associated with asthma risk, a large fraction of heritability remains unexplained, especially in Chinese individuals.

Methods: To identify genetic risk factors for asthma in a Han Chinese population, 211 asthma-related genes were first selected based on database searches. The genes were then sequenced for subjects in a Discovery Cohort (284 asthma patients and 205 older healthy controls) using targeted next-generation sequencing. Bioinformatics analysis and statistical association analyses were performed to reveal the associations between rare/common variants and asthma, respectively. The identified common risk variants underwent a validation analysis using a Replication Cohort (664 patients and 650 controls).

Results: First, we identified 18 potentially functional rare loss-of-function (LOF) variants in 21/284 (7.4%) of the asthma cases. Second, using burden tests, we found that the asthma group had nominally significant (p < 0.05) burdens of rare nonsynonymous variants in 10 genes. Third, 23 common single-nucleotide polymorphisms were associated with the risk of asthma, 7/23 (30.4%) and 9/23 (39.1%) of which were modestly significant (p < 9.1 × 10 ) in the Replication Cohort and Combined Cohort, respectively. According to our cumulative risk model involving the modestly associated alleles, middle- and high-risk subjects had a 2.0-fold (95% CI: 1.621-2.423, p = 2.624 × 10 ) and 6.0-fold (95% CI: 3.623-10.156, p = 7.086 × 10 ) increased risk of asthma, respectively, compared with low-risk subjects.

Conclusion: This study revealed novel rare and common genetic risk factors for asthma, and provided a cumulative risk model for asthma risk prediction and stratification in Han Chinese individuals.
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http://dx.doi.org/10.1002/jcla.23813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183914PMC
June 2021

DDX19A Promotes Metastasis of Cervical Squamous Cell Carcinoma by Inducing NOX1-Mediated ROS Production.

Front Oncol 2021 22;11:629974. Epub 2021 Apr 22.

Department of Gynecology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.

The major obstacle to treat cervical squamous cell carcinoma (CSCC) is the high prevalence of metastasis, which severely affects 5-year survival rate and quality of life for cancer patients. The DEAD-box helicase family has been reported to be a critical mediator in the development and metastasis of various cancers. DEAD-box helicase 19A (DDX19A) is a member of the DEAD-box helicase family; however, its functional role in CSCC is unclear. In this study, bioinformatics analysis of clinical samples from public databases demonstrated that the expression of DDX19A was elevated in CSCC tissues and that high expression of DDX19A was positively correlated with metastasis and poor clinical outcome. Functionally, we found that DDX19A promoted CSCC cell migration and invasion and lung metastasis . Mechanistically, overexpression of DDX19A increased NADPH oxidase 1 (NOX1) expression, enhanced reactive oxygen species (ROS) production, and induced the migration and invasion of CSCC cells. Rescue experiments revealed that DDX19A-induced CSCC functional alterations were dependent on NOX1 and that DDX19A-promoted CSCC metastasis was abrogated upon the inhibition of ROS. Our results demonstrated that DDX19A could promote CSCC metastasis by inducing NOX1-mediated ROS production and that blockage of the NOX1/ROS axis might serve as a potential therapeutic target for patients with DDX19A-overexpressed CSCC.
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http://dx.doi.org/10.3389/fonc.2021.629974DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100682PMC
April 2021

Transcriptome Analysis Reveals the Genes Involved in Growth and Metabolism in Muscovy Ducks.

Biomed Res Int 2021 17;2021:6648435. Epub 2021 Apr 17.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Muscovy ducks are among the best meat ducks in the world. The objective of this study was to identify genes related to growth metabolism through transcriptome analysis of the ileal tissue of Muscovy ducks. Duck ileum samples with the highest (H group, = 5) and lowest (L group, = 5) body weight were selected from two hundred 70-day-old Muscovy ducks for transcriptome analysis by RNA sequencing. In the screening of differentially expressed genes (DEGs) between the H and L groups, a total of 602 DEGs with a fold change no less than 2 were identified, among which 285 were upregulated and 317 were downregulated. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that glutathione metabolism, pyrimidine metabolism, and protein digestion and absorption processes played a vital role in regulating growth and metabolism. The results showed that 7 genes related to growth and metabolism, namely, , , , , , , and , were significantly expressed in group H, which was consistent with the phenotype results. The validation of these 7 genes using real-time quantitative PCR results indicated that the expression level of was significantly different between the H and L groups ( < 0.05). This study provides a theoretical basis for exploring the influence of the ileum on growth and metabolism in ducks.
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http://dx.doi.org/10.1155/2021/6648435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077732PMC
May 2021

Roles of kinesin superfamily proteins in colorectal cancer carcinogenesis (Review).

Oncol Rep 2021 Jul 6;46(1). Epub 2021 May 6.

The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Beijing Hospital, National Center of Gerontology, National Health Commission; Institute of Geriatric Medicine, Chinese Academy of Medical Science, Beijing 100730, P.R. China.

Colorectal cancer (CRC), a commonly occurring carcinoma, now ranks the second in terms of cancer‑associated deaths around the world. Among the numerous factors that contribute to CRC tumor progression, a class of motor proteins known as the kinesins has been found to play a vital role. Kinesins are responsible for the intracellular trafficking of functional proteins, organelles and biomacromolecules along microtubules. Dysregulation of kinesins has been revealed to influence the cell cycle to cause abnormal cell growth and affect cell adhesion to promote epithelial‑mesenchymal transition in breast, bladder, ovarian and prostate cancer. Studies on the function of kinesins in CRC have also been performed, although, to the best of our knowledge, little is known about the underlying mechanisms of kinesins in CRC progression. The present review outlines the roles played by different kinesins in CRC carcinogenesis, mainly discussing the most studied subfamilies (kinesin 3‑6, 8, 10, 11 and 13), This review aims to illustrate the functions of kinesins in CRC cell growth, cancer metastasis and chemoresistance to provide insights regarding kinesins as potential targets for determining CRC prognosis and selecting therapy.
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http://dx.doi.org/10.3892/or.2021.8072DOI Listing
July 2021

Recent Progress in the Diagnosis and Precise Nanocarrier-Mediated Therapy of Inflammatory Bowel Disease.

J Inflamm Res 2021 29;14:1701-1716. Epub 2021 Apr 29.

Department of General Surgery, Xinqiao Hospital, Army Medical University, Chongqing, People's Republic of China.

The effective colon drug delivery remains to be an international frontier research in inflammatory bowel disease (IBD) therapy. The exploration and research of nanocarrier-based nanomedicine with great potential brings new opportunities for IBD therapy and diagnoses. Functional nanocarriers with varying morphology and characteristics can not only effectively avoid the destruction of the complex gastrointestinal (GI) tract microenvironment but also endow drugs with target therapy and improved bioavailability, thus elevating therapeutic efficacy. In this review, we illustrated several challenges in IBD therapy, then emphasis on some latest research progress of nanoparticles based therapy of oral administration, rectal administration and parenteral administration, as well as IBD diagnoses. Finally, we described the future perspective of nanocarriers in the treatment and diagnoses of IBD.
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http://dx.doi.org/10.2147/JIR.S304101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092629PMC
April 2021