Publications by authors named "Hua Li"

223 Publications

Superwettable cellulose acetate-based nanofiber membrane with spider-web structure for highly efficient oily water purification.

Authors:
Doudou Ning Zhaoqing Lu Cuiyu Tian Ning Yan Fan Xie Nan Li Li Hua

Int J Biol Macromol 2023 Sep 17;253(Pt 3):126865. Epub 2023 Sep 17.

College of Bioresources Chemical and Materials Engineering, Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper Development, National Demonstration Center for Experimental Light Chemistry Engineering Education, Shaanxi University of Science & Technology, Xi'an 710021, China.

Electrospinning nanofibers membrane has received much attention to remove the insoluble oil from the sewage, while the poor mechanical strength and low oil/water separation efficiency of membranes limit their practical application. Here, we prepared a superwettable deacetylated cellulose acetate (d-CA)-based electrospinning nanofibers membrane simply dipped by bacterial cellulose (BC) and cross-linked with citric acid (CCA) to construct the spider-web structure spontaneously. Compared with the pristine d-CA membrane, the obtained d-CA/BC@CCA membrane exhibits the remarkable oil/water separation performance. The flux and separation efficiency of n-hexane/water emulsion without (SFE) and with (SSE) emulsifier for d-CA/BC@CCA membrane are 9364 L·m·h·bar, 98.34 % and 5479 L·m·h·bar, 99.39 %, respectively, which are mainly attributed to the improved hydrophilicity of its surface and the decreased pore sizes caused by the unique spider-web structure. In addition, d-CA/BC@CCA membrane also possesses the outstanding mechanical properties, the better cycle stability, as well as the excellent durability. This study provides a novel strategy for the construction of the high-performance oil/water separation membrane.
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http://dx.doi.org/10.1016/j.ijbiomac.2023.126865DOI Listing
September 2023

Preparation of sludge-based biochar loaded with ferromanganese and its removal mechanism of tetracycline hydrochloride.

Authors:
Li Hua Lumengfei Yu Fanglin Dang Hui Zhao Ting Wei

Environ Sci Pollut Res Int 2023 Aug 30. Epub 2023 Aug 30.

College of Environmental Science and Technology, Shaanxi University of Science and Technology, Xi'an, 710021, Shaanxi, China.

To remove the serious contamination caused by tetracycline hydrochloride, this paper uses the method of impregnation followed by pyrolysis to prepare ferromanganese-loaded sludge-based biochar and investigate its effectiveness in removing tetracycline hydrochloride. The material was characterized by field emission SEM, FTIR, and X-ray diffraction analysis. The possible reaction mechanisms involved in the removal of tetracycline were deduced based on the determination of Mn during the reaction process and XPS characterization of materials before and after the reaction, and analysis of degradation intermediates and reaction pathways during tetracycline hydrochloride degradation was discussed. The results showed that the highest removal rate of 90.71% was achieved at a Fe-to-Mn ratio of 2:1 for the Fe-to-Mn-loaded sludge-based biochar. XPS characterization before and after the reaction showed that the valence state of Fe did not change significantly and was stable, while Mn partially changed to Mn and a redox reaction occurred. The changes in Mn concentration during the reaction showed that the degradation of tetracycline hydrochloride was mainly dominated by MnO. The LC-MS analysis revealed eight intermediates in the degradation of tetracycline, and two possible reaction pathways existed.
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http://dx.doi.org/10.1007/s11356-023-29558-7DOI Listing
August 2023

The efficacy and safety of patent Foramen Ovale Closure for Refractory Epilepsy (PFOC-RE): a prospectively randomized control trial of an innovative surgical therapy for refractory epilepsy patients with PFO of high-grade right-to-left shunt.

Authors:
Ji Shuming Li Hua Tang Yusha Chen Lei

BMC Neurol 2023 Jul 27;23(1):282. Epub 2023 Jul 27.

Department of Neurology, West China Hospital, Joint Research Institution of Altitude Health, Sichuan University, Chengdu, 610044, China.

Background: A significant proportion of patients with epilepsy have an unknown etiology and lack effective targeted therapeutic drugs. Patent Foramen Ovale (PFO) induces hypoxia and microembolism, leading to cerebral neurological dysfunction and increased epilepsy risk. This study aims to assess the efficacy and safety of PFO closure for relieving epileptic seizures in patients with refractory epilepsy associated with PFO.

Methods/design: Recruitment takes place at the West China Hospital of Sichuan University, China, for an open-label, randomized controlled clinical trial. The trial will include 110 patients with refractory epilepsy and PFO. Disease diagnoses will conform to the diagnostic criteria of the International League Against Epilepsy (ILAE) for refractory epilepsy and the American Society of Echocardiography (ASE) for PFO. Refractory epilepsy and high-grade right-to-left shunt (RLS) of the PFO will be further diagnosed using 24-hour video electroencephalogram and transthoracic echocardiography with contrast injection, respectively. Eligible participants require a secondary or higher volume of RLS.

Trial Registration: Chinese Clinical Trial Registry (ChiCTR2200065681). Registered on November 11, 2022.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373383PMC
http://dx.doi.org/10.1186/s12883-023-03317-0DOI Listing
July 2023

MALDI-TOF MS Identification of some Clinically-Relevant Filamentous Fungi with the Direct Smear Method, a Simple Sample Preparation Method.

Authors:
Li Hua Zhitao Yang Chenlu Xiao

Clin Lab 2023 Jul;69(7)

Background: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful technique for the identification of microorganisms. The technique normally requires a sample preparation procedure before instrumental analysis, which can be somewhat labor-intensive when numbers of samples are large. The direct smear method, in which samples are directly smeared on the sample plates and subsequently subjected to instrumental analyses, can save time and is less labor-intensive. However, the method has rarely been tested on filamentous fungi, although it has been successfully used in the identification of bacteria and yeasts. In the present study, we examined the method using clinically-collected filamentous fungi.

Methods: Three hundred forty-eight isolates of filamentous fungi representing 9 species collected from body fluids of patients were analyzed on the VITEK MS version 3.0 system, a popular commercial MALDI-TOF MS system, using the direct smear method. For those misidentified or unidentified, the samples were retested. All fungal species were determined through DNA sequencing.

Results: Among 334 isolates that were included in the database of the VITEK system, 286 (85.6%) samples were correctly identified. After retesting, the rate of correct identification increased to 91.0%. Aspergillus fumigatus exhibited a 95.2% rate of correct identification before retesting, whereas Aspergillus niger showed the rate at only 46.5% (58.1% even with retesting).

Conclusions: The direct smear method could be used in the identification of filamentous fungi found in body fluids of patients by MALDI-TOF MS at good rates of correct identification. The method is simple and time-saving, and deserves further evaluation.
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http://dx.doi.org/10.7754/Clin.Lab.2022.221003DOI Listing
July 2023

Arsenic Inhibits Proliferation and Induces Autophagy of Tumor Cells in Pleural Effusion of Patients with Non-Small Cell Lung Cancer Expressing EGFR with or without Mutations via PI3K/AKT/mTOR Pathway.

Authors:
Jianhua Mao Xiaoqian Shi Li Hua Menghang Yang Yan Shen Zheng Ruan Bing Li Xiaodong Xi

Biomedicines 2023 Jun 15;11(6). Epub 2023 Jun 15.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, Collaborative Innovation Center of Hematology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

To clarify whether arsenic could exert inhibitory effects on tumor cells in pleural effusions of patients with non-small cell lung cancer (NSCLC), 36 NSCLC pleural effusion samples were collected from Changzheng Hospital and Ruijin Hospital, from 2019 to 2022. The genotype of epidermal growth factor receptor () was identified. Tumor cells were isolated and treated with arsenic trioxide (ATO) or/and gefitinib. Additionally, six patients were intrapleurally administrated with ATO. Results showed that 25 samples bore wild type (WT) and 11 harbored mutations, including 6 with L858R, 3 with ΔE746-A750, and 2 with T790M. ATO diminished the number of tumor cells from patients with WT and mutant , down-regulated the expression or phosphorylation of EGFR, pmTOR, PI3K, PTEN, and p4E-BP1, and up-regulated the expression of LC3. Immunofluorescent experiments showed that ATO enhanced LC3 and P62. By contrast, gefitinib was only effective in those harboring sensitizing mutations. Notably, in patients with intrapleural ATO injection, the pleural effusion underwent a bloody to pale yellow color change, the volume of the pleural effusion was reduced, and the number of the tumor cells was significantly reduced. In conclusion, arsenic is effective against NSCLC with various genotypes in vitro and in vivo, and potentially circumvents gefitinib resistance.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295848PMC
http://dx.doi.org/10.3390/biomedicines11061721DOI Listing
June 2023

Performance of heavy metal-immobilizing bacteria combined with biochar on remediation of cadmium and lead co-contaminated soil.

Authors:
Ting Wei Han Gao Fengqiu An Xiulian Ma Li Hua Junkang Guo

Environ Geochem Health 2023 Aug 19;45(8):6009-6026. Epub 2023 May 19.

School of Environmental Science and Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, People's Republic of China.

Heavy metal pollution of soil has become a public concern worldwide since it threats food safety and human health. Sustainable and environmental-friendly remediation technology is urgently needed. Therefore, we investigated the properties and heavy metal removal ability of Enterobacter asburiae G3 (G3), Enterobacter tabaci I12 (I12), and explored the feasibility of remediation Cd, Pb co-contaminated soil by the combination of G3/I12 and biochar. Our results indicated that both strains are highly resistant to Cd, Pb and maintain plant growth-promoting properties. The removal efficiency of G3 for Cd and Pb were 76.79-99.43%, respectively, while the removal efficiency of I12 for Cd and Pb were 62.57-99.55%, respectively. SEM-EDS and XRD analysis revealed that the morphological and structural changes occurred upon heavy metal exposure, metal precipitates were also detected on cell surface. FTIR analysis indicated that functional groups (-OH, -N-H, -C = O, -C-N, -PO) were involved in Cd/Pb immobilization. Application of the bacteria, biochar, or their combination decreased the acid-extractable Cd, Pb in soil while increased the residual fractions, meanwhile, the bioavailability of both metal elements declined. Besides, these treatments increased soil enzyme (sucrase, catalase and urease) activity and accelerated pakchoi growth, heavy metal accumulation in pakchoi was depressed upon bacteria and/or biochar application, and a synergistic effect was detected when applying bacteria and biochar together. In BC + G3 and BC + I12 treated plants, the Cd and Pb accumulation decreased by 24.42% and 52.19%, 17.55% and 47.36%, respectively. Overall, our study provides an eco-friendly and promising in situ technology that could be applied in heavy metal remediation.
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http://dx.doi.org/10.1007/s10653-023-01605-9DOI Listing
August 2023

Association of serum anti-carbamylated protein antibodies with disease activity and bone loss in rheumatoid arthritis.

Authors:
Liuqing Wang Li Hua Xuelian Hong Fang Chen Hongwei Du

Clin Chim Acta 2023 Jun 29;546:117371. Epub 2023 Apr 29.

Department of Rheumatology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jin Hua 321000, China. Electronic address:

Background: We investigated the association of serum anti-carbamylated protein (anti-CarP) antibodies with disease activity and bone loss in rheumatoid arthritis (RA).

Methods: The serum anti-CarP antibody concentrations of RA and non-RA patients and healthy controls were determined by enzyme-linked immunosorbent assay (ELISA) and then compared. The diagnostic value of anti-CarP antibodies in RA was determined by the receiver operating characteristic curve. Patients with RA and bone erosions were evaluated using ultrasound examinations. Ultrasonography was performed using a semiquantitative scale. The serum receptor activator of nuclear factor Κ-Β ligand (RANKL) concentrations were measured by ELISA to focus on bone loss.

Results: Peripheral serum anti-CarP antibody concentrations in patients with RA were significantly higher than those in patients without RA and in healthy controls and were positively correlated with disease activity. Anti-CarP antibody concentrations were significantly increased in patients with anti-CCP-positive RA. Positive correlation were found between anti-CarP and RANKL. Increased serum anti-CarP antibodies in women with postmenopausal osteoporosis (OP).

Conclusions: Anti-CarP antibodies are associated with RA disease activity and may play an important role in bone loss associated with RA. The concentration of anti-CarP antibodies may be beneficial in the early diagnosis of RA, thus supporting its potential as a novel disease biomarker.
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http://dx.doi.org/10.1016/j.cca.2023.117371DOI Listing
June 2023

Efficacy of Children Neuropsychological and Behavioral Scale in Screening for Autism Spectrum Disorders through a Combination of Developmental Surveillance.

Authors:
Jin-Hui Li Jin-Zhu Zhao Li Hua Xiao-Lin Hu Li-Na Tang Ting Yang Tian-Yi He Chen Hu Xiao-Qun Yu Ting-Ting Xiong Dan-Dan Wu Yan Hao

Curr Med Sci 2023 Jun 28;43(3):592-601. Epub 2023 Apr 28.

Division of Child Healthcare, Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Objective: This study aimed to explore the clinical value of Children Neuropsychological and Behavioral Scale-Revision 2016 (CNBS-R2016) for Autism Spectrum Disorder (ASD) screening in the presence of developmental surveillance.

Methods: All participants were evaluated by the CNBS-R2016 and Gesell Developmental Schedules (GDS). Spearman's correlation coefficients and Kappa values were obtained. Taking GDS as a reference assessment, the performance of the CNBS-R2016 for detecting the developmental delays of children with ASD was analyzed with receiver operating characteristic (ROC) curves. The efficacy of the CNBS-R2016 to screen for ASD was explored by comparing Communication Warning Behavior with Autism Diagnostic Observation Schedule, Second Edition (ADOS-2).

Results: In total, 150 children aged 12-42 months with ASD were enrolled. The developmental quotients of the CNBS-R2016 were correlated with those of the GDS (r=0.62-0.94). The CNBS-R2016 and GDS had good diagnostic agreement for developmental delays (Kappa=0.73-0.89), except for Fine Motor. There was a significant difference between the proportions of Fine Motor, delays detected by the CNBS-R2016 and GDS (86.0% vs. 77.3%). With GDS as a standard, the areas under the ROC curves of the CNBS-R2016 were above 0.95 for all the domains except Fine Motor, which was 0.70. In addition, the positive rate of ASD was 100.0% and 93.5% when the cut-off points of 7 and 12 in the Communication Warning Behavior subscale were used, respectively.

Conclusion: The CNBS-R2016 performed well in developmental assessment and screening for children with ASD, especially by Communication Warning Behaviors subscale. Therefore, the CNBS-R2016 is worthy of clinical application in children with ASD in China.
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http://dx.doi.org/10.1007/s11596-023-2698-5DOI Listing
June 2023

Self efficacy associated with regression from pregnancy-related pelvic girdle pain and low back pain following pregnancy.

Authors:
Dai Chunmei Chen Yong Gong Long Tan Mingsheng Li Hua Yi Ping

BMC Pregnancy Childbirth 2023 Feb 21;23(1):122. Epub 2023 Feb 21.

Department of Orthopedic, China-Japan Friendship Hospital, No. 2 Yinhuayuan East Street, Chaoyang, Beijing, 100029, China.

Background: Self-efficacy, one's ability to deal with pain, disability, and other symptoms through self-management techniques, positively affect the quality of life in patients with chronic diseases. Pregnancy-related back pain is a common musculoskeletal disorder pre- and postnatally. Hence, the study aimed to determine whether self-efficacy is associated with the development of back pain during pregnancy.

Methods: Between February 2020 and February 2021, a prospective case-control study was performed. Women with back pain were included. The self efficacy was assessed by the Chinese version of the General Self-efficacy Scale (GSES). Pregnancy-related back pain was measured using a self-reported scale. No regression from pregnancy-related back pain is defined as a recurrent or persistent pain score ≥ 3 over a week around 6 months postpartum. Women experiencing back pain during pregnancy are classified according to whether having a regression. This problem can be divided into pregnancy-related low back pain (LBP) and posterior girdle pain (PGP). The differences in variables were compared between groups.

Results: A total of 112 subjects have completed the study finally. These patients were followed up with an average of 7.2 months after childbirth ranging from six to 8 months. 31 subjects (27.7%) of the included women did not report regression 6 months postpartum. The mean self efficacy was 25.2 (SD:10.6). Patients with no regression tended to be older (LBP:25.9 ± 7.2 vs.31.8 ± 7.9, P = 0.023; PGP: 27.2 ± 7.9 vs. 35.9 ± 11.6, P < 0.001*), have a lower self efficacy (LBP:24.2 ± 6.6 vs.17.7 ± 7.1, P = 0.007; PGP: 27.6 ± 6.8 vs. 22.5 ± 7.0, P = 0.010), and need high daily physical demand in their vocations (LBP:17.4% vs. 60.0%, P = 0.019; PGP: 10.3% vs. 43.8%, P = 0.006) when compared to those with regression. Multivariate logistic analysis shows that risk factors for no regression from pregnancy-related back pain included LBP (OR = 2.36, 95%CI = 1.67-5.52, P < 0.001), pain ratings of the onset of back pain during pregnancy≥3(OR = 2.23, 95%CI = 1.56-6.24, P = 0.004), low self efficacy (OR = 2.19, 95%CI = 1.47-6.01, P < 0.001), and high daily physical demand in their vocations (OR = 2.01, 95%CI = 1.25-6.87, P = 0.001).

Conclusions: Low self efficacy makes the women experience about two-fold risk to experience no regression from pregnancy-related back pain. Evaluation for self efficacy is simple enough to be used to improve perinatal health.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942289PMC
http://dx.doi.org/10.1186/s12884-023-05393-zDOI Listing
February 2023

Immunosuppressive reprogramming of neutrophils by lung mesenchymal cells promotes breast cancer metastasis.

Authors:
Zheng Gong Qing Li Jiayuan Shi Peishan Li Li Hua Leonard D Shultz Guangwen Ren

Sci Immunol 2023 Feb 17;8(80):eadd5204. Epub 2023 Feb 17.

Jackson Laboratory, Bar Harbor, ME 04609, USA.

Neutrophils, the most abundant innate immune cells, function as crucial regulators of the adaptive immune system in diverse pathological conditions, including metastatic cancer. However, it remains largely unknown whether their immunomodulatory functions are intrinsic or acquired within the pathological tissue environment. Here, using mouse models of metastatic breast cancer in the lungs, we show that, although neutrophils isolated from bone marrow (BM) or blood are minimally immunosuppressive, lung-infiltrating neutrophils are robustly suppressive of both T cells and natural killer (NK) cells. We found that this tissue-specific immunosuppressive capacity of neutrophils exists in the steady state and is reinforced by tumor-associated inflammation. Acquisition of potent immunosuppression activity by lung-infiltrating neutrophils was endowed by the lung-resident stroma, specifically CD140a mesenchymal cells (MCs) and largely via prostaglandin-endoperoxide synthase 2 (PTGS2), the rate-limiting enzyme for prostaglandin E (PGE) biosynthesis. MC-specific deletion of or pharmacological inhibition of PGE receptors reversed lung neutrophil-mediated immunosuppression and mitigated lung metastasis of breast cancer in vivo. These lung stroma-targeting strategies substantially improved the therapeutic efficacy of adoptive T cell-based immunotherapy in treating metastatic disease in mice. Collectively, our results reveal that the immunoregulatory effects of neutrophils are induced by tissue-resident stroma and that targeting tissue-specific stromal factors represents an effective approach to boost tissue-resident immunity against metastatic disease.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067025PMC
http://dx.doi.org/10.1126/sciimmunol.add5204DOI Listing
February 2023

Analysis of the clinical features and risk factors of device-related pressure injuries in the operating room.

Authors:
Ling-Yu Ma Hong-Lin Chen Hai-Yan Gu Li Hua Xiao-Mei Gao

Int Wound J 2023 Mar 19;20(3):706-715. Epub 2022 Aug 19.

Department of Operating Room, Second Affiliated Hospital of Nantong University, Nantong, China.

To describe the clinical features and risk factors of device-related pressure injuries (DRPIs) in the operating room. The clinical features of the DRPIs in patients undergoing elective surgery in a tertiary hospital in 2020 were investigated through prospective data collection. A DRPI-related questionnaire was designed for the patients, and those who did not experience any DRPI were selected according to a ratio of 1:2. Logistic regression analysis was performed in terms of the independent risk factors of operating-room DRPIs. A P-value of <.05 indicated a statistically significant difference. The incidence of operating-room DRPIs was 0.56%, and the proportion of stage I injuries was 73.53%. The injury-related devices included vital monitoring devices (31.62%), auxiliary therapy devices (27.94%), therapy devices (19.12%), and dressings (3.67%). Non-bone protuberances, such as the upper arms and thighs, were common injury sites. The patients' body mass index, mean arterial pressure, and instrument action time were independent risk factors for the operating-room DRPIs. To reduce the incidence of operating-room DRPIs, it is of great clinical significance to focus on the characteristics of the surgical patients and the types of surgery-related devices used and to take personalised preventive measures based on the relevant risk factors.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9927895PMC
http://dx.doi.org/10.1111/iwj.13912DOI Listing
March 2023

Research on outpatient capacity planning combining lean thinking and integer linear programming.

Authors:
Li Hua Mu Dongmei Yang Xinyu Zhang Xinyue Wang Shutong Wang Dongxuan Peng Hao Wang Ying

BMC Med Inform Decis Mak 2023 Feb 13;23(1):32. Epub 2023 Feb 13.

School of Public Health, Jilin University, Changchun, Jilin, China.

Background: The size and cost of outpatient capacity directly affect the operational efficiency of a whole hospital. Many scholars have faced the study of outpatient capacity planning from an operations management perspective.

Objective: The outpatient service is refined, and the quantity allocation problem of each type of outpatient service is modeled as an integer linear programming problem. Thus, doctors' work efficiency can be improved, patients' waiting time can be effectively reduced, and patients can be provided with more satisfactory medical services.

Methods: Outpatient service is divided into examination and diagnosis service according to lean thinking. CPLEX is used to solve the integer linear programming problem of outpatient service allocation, and the maximum working time is minimized by constraint solution.

Results: A variety of values are taken for the relevant parameters of the outpatient service, using CPLEX to obtain the minimum and maximum working time corresponding to each situation. Compared with no refinement stratification, the work efficiency of senior doctors has increased by an average of 25%. In comparison, the patient flow of associate senior doctors has increased by an average of 50%.

Conclusion: In this paper, the method of outpatient capacity planning improves the work efficiency of senior doctors and provides outpatient services for more patients in need; At the same time, it indirectly reduces the waiting time of patients receiving outpatient services from senior doctors. And the patient flow of the associate senior doctors is improved, which helps to improve doctors' technical level and solve the problem of shortage of medical resources.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9924205PMC
http://dx.doi.org/10.1186/s12911-023-02106-6DOI Listing
February 2023

Thymus transplantation regulates blood pressure and alleviates hypertension-associated heart and kidney damage via transcription factors FoxN1 pathway.

Authors:
Xianliang Dai Jian Zhao Li Hua Hui Chen Chun Liang

Int Immunopharmacol 2023 Mar 2;116:109798. Epub 2023 Feb 2.

Department of Cardiology, Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China. Electronic address:

Previous studies have found that thymus is involved in the process of hypertension. However, whether thymus transplantation alleviates target organ damage in hypertensive mice remains unknown. The aim of this study was to evaluate the effects of thymus transplantation on blood pressure and target organ changes in mice with hypertension. Mice were randomly divided into normal control group (Con), hypertensive group (HTN) and thymus transplantation group (HTN + Trans). Thymus of neonatal mice was transplanted into the renal capsule of the transplantation group. After transplantation, the mouse tail noninvasive pressure was measured and heart function was evaluated weekly. Then mice were euthanized and organs or tissues were harvested at 4 weeks post-transplantation. The blood pressure of HTN + Trans group was lower than that in the HTN group. The expression of FoxN1, Aire, ATRAP, thymosin β4 and the content of sjTREC in thymus of HTN group was decreased and the number of naïve T cells in HTN group was lower compared with other two groups. The ratio of cTEC/mTEC in HTN group was higher than that in Con group and lower than that in HTN + Trans group. Cardiac pathology showed cardiac hypertrophy and fibrosis in HTN group whereas thymus transplantation improved heart function and structure. Altogether, our findings demonstrated thymus transplantation could improve thymus function of hypertensive mice, which increased the expression of thymus transcription factor FoxN1, affected the proportion of T cell subsets, and increased thymosin β4 thereby reducing blood pressure and reversing the progression of target organ damage.
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http://dx.doi.org/10.1016/j.intimp.2023.109798DOI Listing
March 2023

Efficacy and safety of belimumab in lupus nephritis: A retrospective study.

Authors:
Zhaowei Zhang Jiayin Chen Hongwei Du Li Hua

Int J Clin Pharmacol Ther 2023 Jan 28. Epub 2023 Jan 28.

Objective: This retrospective analysis was to examine the efficacy and safety of belimumab in lupus nephritis in China.

Materials And Methods: 25 patients who were regularly followed up every 3 months in our hospital in China were included. All patients were diagnosed as having lupus nephritis and received belimumab to complement standard therapy. The primary outcomes 24-hour proteinuria, complement level, estimated glomerular filtration rate (eGFR), SELENA-SLEDAI scores, prednisone daily dose, and adverse reactions were recorded at 12, 24, 36, and 48 weeks.

Results: The SELENA-SLEDAI scores and 24-hour urine protein of the 25 patients decreased visibly from baseline 15.96 ± 3.02, 1.52 ± 1.72 to 9.52 ± 2.66 (p < 0.01), 0.5 ± 0.2 (p < 0.05) at 48 weeks, the eGFR of the 25 patients increased from 76.45 ± 22.2 to 85.48 ± 19.26 (p < 0.01) at 48 weeks, complement levels also showed a trend to increase. One patient experienced renal flare at 48 weeks, and the level of the patient's 24-hour proteinuria had been > 0.7 g at 6 months; this may be a strong predictive factor for further renal response at 12 months. Belimumab added to the standard therapy also improved the hematologic complications caused by systemic lupus erythematosus in 2 patients with a lower glucocorticoid dose. There were no serious adverse events.

Conclusion: Belimumab may be effective and safe in lupus nephritis even with regard to hematologic complications.
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http://dx.doi.org/10.5414/CP204323DOI Listing
January 2023

What's the role of thymus in diabetes mellitus?

Authors:
Xianliang Dai Li Hua Hui Chen Qiheng Li Wansheng Chen Chun Liang

Int Immunopharmacol 2023 Mar 24;116:109765. Epub 2023 Jan 24.

Department of Cardiology, Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China. Electronic address:

Diabetes mellitus is considered as an autoimmune inflammatory and age-related disease. As an important immune organ, the thymus is involved in the immune response and inflammatory response process. Therefore, there may be a link between changes in thymus function and diabetes. Based on previous studies, we hypothesized that thymus dysfunction due to aging and other reasons leads to changes in the generation of various inflammatory-immune cells and inflammatory cytokines that regulate insulin resistance, and then participates in the development of diabetes and its complications. Therefore, thymus may be a key factor in diabetes and complications, and it may be a promising therapeutic strategy to improve the thymus function for patients with diabetes. The purpose of this review is to summarize and discuss recent advances in the influence of thymus function on diabetes, especially its potential mechanisms.
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http://dx.doi.org/10.1016/j.intimp.2023.109765DOI Listing
March 2023

Correction to: Clinical features and high-risk indicators of central nervous system involvement in primary Sjögren's syndrome.

Authors:
Wei Fan Jennefer Par-Young Kaiyan Li Yi Zhang Pingping Xiao Li Hua Lin Leng Xuyan Chen Richard Bucala

Clin Rheumatol 2023 Feb;42(2):643

Department of Internal Medicine, Section of Rheumatology, Allergy & Immunology, Yale University School of Medicine, CT, 06520, New Haven, USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873696PMC
http://dx.doi.org/10.1007/s10067-022-06488-2DOI Listing
February 2023

Walking on inclines alters the gait characteristics in patients with acute stroke.

Authors:
Zhang Xin Chi Zhang Hu Gongwei Zhou Zhe Li Hua

Gait Posture 2023 Feb 25;100:49-56. Epub 2022 Nov 25.

Rehabilitation Center, Shanghai First Rehabilitation Hospital, Shanghai/ School of Medicine, China.

Background: Stroke leads to severe difficulties in daily activities, even when performing a simple task, such as walking from one point to another. The first apparent compensatory strategy in stroke survivors during walking is to slow down the walking speed. Slowing down the walking speed directly reduces the step length and cadence and further increases the stance phase, double, and support time. These alterations are to develop a compensatory strategy; however, this strategy generally leads to joint injuries and increases the potential risks of falls.

Research Question: A review strongly suggests that walking on the inclines may enhance this compensatory strategy. Therefore, this study attempted to extend the current knowledge to understand the fundamental gait control in patients with acute stroke during walking on inclines.

Results: These results showed that (1) compared to healthy control, patients with acute stroke demonstrated different gait controls during walking on inclines, (2) the gait performance was enhanced when stroke patients were instructed on different inclines, and (3) the asymmetric gait pattern was reduced by walking on inclines.

Significance: This study concluded that walking on inclines enhances gait performance (greater spatial but smaller temporal gait characteristics) and reduces spatial gait asymmetry.
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http://dx.doi.org/10.1016/j.gaitpost.2022.11.011DOI Listing
February 2023

A Validated UHPLC-MS/MS Method for Determination of Nalbuphine in Human Plasma and Application for Pharmacokinetic Study of Patients Undergoing General Anesthesia.

Authors:
Xiaonan Gao Xuyang Nie Jinglin Gao Tianfang Heng Yuqi Zhang Li Hua Yaqi Sun Zhangying Feng Li Jia Mingxia Wang

J Chromatogr Sci 2022 Dec 3. Epub 2022 Dec 3.

Department of Clinical Pharmacology, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Shijiazhuang 050011, People's Republic ofChina.

Nalbuphine was a semisynthetic opioid analgesic widely used in the treatment of both acute and chronic pain. We developed and validated a rapid, simple and sensitive method by ultra-performance liquid chromatography-tandem mass spectrometry (MS/MS) for the simultaneous quantitation of nalbuphine in human plasma, and we reported the pharmacokinetic features of patients during general anesthesia for abdominal surgery. Sample separation was achieved on a Kinetex Phenyl-Hexyl column (50 × 2.1 mm, 1.7 μm) after simple protein precipitation with acetonitrile. The mobile phase was composed of acetonitrile and 3 mM of ammonium acetate aqueous solution with 0.1% formic acid. Gradient elution was used in 4.5 min with a flow rate of 0.5 mL/min at 40°C. MS detection using AB Sciex QTRAP 5500 mass spectrometer was characterized by electrospray ionization for positive ions in multiple reaction monitoring mode. Quantitative ion pairs were m/z 358.4 → 340.1 for nalbuphine and m/z 340.0 → 268.3 for nalmefene, which were used as the internal standard (IS). The calibration curves showed good linearity (r2>0.99) over concentration range of 0.1-500 ng/mL. The intra-and inter-batch precisions were within 10.67%, and accuracy ranged from 94.07 to 105.34%. The IS-normalized matrix factors were 1.02-1.03 with RSD% (≤5.82%). The recoveries ranged from 101.09 to 106.30%. In conclusion, a rapid, simple, sensitive and economical analytical method was developed and validated to detect the concentration in plasma samples obtained from patients receiving nalbuphine intravenous injection and was successfully applicated to human pharmacokinetic studies of nalbuphine.
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http://dx.doi.org/10.1093/chromsci/bmac094DOI Listing
December 2022

Directed evolution engineering to improve activity of glucose dehydrogenase by increasing pocket hydrophobicity.

Authors:
Li Hua Bao Qianqian Zhao Jianfeng Xu Yinbiao Yang Shengyu Xue Weishi Sun Yang Liu Yupeng

Front Microbiol 2022 9;13:1044226. Epub 2022 Nov 9.

School of Life Sciences, Institute of Microbial Engineering, Henan University, Kaifeng, China.

Glucose dehydrogenase (GDH) is a NAD(P) dependent oxidoreductase, which is useful in glucose determination kits, glucose biosensors, cofactor regeneration, and biofuel cells. However, the low efficiency of the catalysis hinders the use of GDH in industrial applications. In this study, an analysis of interactions between eight GDH mutants and NADP is powered by AlphaFold2 and Discovery Studio 3.0. The docking results showed that more hydrogen bonds formed between mutants, such as P45A and NADP, which indicated that these mutants had the potential for high catalytic efficiency. Subsequently, we verified all the mutants by site-directed mutagenesis. It was notable that the enzyme activity of mutant P45A was 1829 U/mg, an improvement of 28-fold compared to wild-type GDH. We predicted the hydrophobicity of the protein-ligand complexes, which was confirmed by an 8-anilino-1-naphthalenesulphonic acid fluorescent probe. The following order of increasing hydrophobicity index was deduced: GDH < N46E < F155Y < P45A, which suggested that the enzyme activity of GDH is positively related to its pocket hydrophobicity. Furthermore, P45A still showed better catalytic ability in organic solvents, reaching 692 U/mg in 10% isopropanol, which was 19-fold that of the wild-type GDH. However, its substrate affinity was affected by organic solvents. This study provides a good theoretical foundation for further improving the catalytic efficiency of GDH.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9681798PMC
http://dx.doi.org/10.3389/fmicb.2022.1044226DOI Listing
November 2022

Prognostic and Clinical Value of the Systemic Immune-Inflammation Index in Biliary Tract Cancer: A Meta-Analysis.

Authors:
Xiulan Peng Xia Wang Li Hua Rui Yang

J Immunol Res 2022 17;2022:6988489. Epub 2022 Nov 17.

Department of Vascular Surgery, The Second Affiliated Hospital of Jianghan University, the Fifth Hospital of Wuhan, Wuhan, Hubei 430000, China.

Previous studies that explored the prognostic and clinical value of the systemic immune-inflammation index (SII) in biliary tract cancer (BTC) had inconsistent results. We conducted this meta-analysis to evaluate the prognostic and clinicopathological role of the SII in biliary tract cancer. Combined analysis demonstrated that high SII levels had worse overall survival (HR = 1.92, 95% CI: 1.66-2.21, < 0.001) than those with low SII levels. And an elevated SII was associated with lymph node metastasis (OR = 1.44, 95% CI = 1.18-1.76; < 0.001), TNM stage (OR = 1.49, 95% CI = 1.05-2.13; = 0.028), and vascular invasion (OR = 1.49, 95% CI = 1.05-2.13; = 0.028). Conversely, no significant association between a high SII and sex or tumor differentiation was found. Our findings demonstrate that high SII levels were correlated with unfavorable survival outcomes among patients with BTC and that they were also correlated with some higher malignancy features of BTC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691295PMC
http://dx.doi.org/10.1155/2022/6988489DOI Listing
November 2022

Clinical features and high-risk indicators of central nervous system involvement in primary Sjögren's syndrome.

Authors:
Wei Fan Jennefer Par-Young Kaiyan Li Yi Zhang Pingping Xiao Li Hua Lin Leng Xuyan Chen Richard Bucala

Clin Rheumatol 2023 Feb 19;42(2):443-451. Epub 2022 Nov 19.

Department of Internal Medicine, Section of Rheumatology, Allergy & Immunology, Yale University School of Medicine, New Haven, CT, 06520, USA.

Background: Evidence for central nervous system involvement in primary Sjögren's syndrome (pSS) patients is controversial and extremely limited. We aimed to describe the clinical profiles and high-risk indicators of primary Sjögren's syndrome (pSS) patients with central nervous system (CNS) involvement (pSS-CNS).

Methods: A total of 412 participants with pSS from a hospital in China from January 2012 to December 2019 were enrolled in the retrospective study. 42 pSS-CNS patients were compared with 370 pSS patients without CNS involvement. The clinical features, laboratory examinations, imaging characteristics, and treatment of the pSS-CNS cases were systematically analyzed. Potential risk factors related to pSS-CNS patients were identified by multivariate logistic regression analysis.

Results: The prevalence of central nervous system involvement in the studied pSS patients was 10.2% (42/412), with 31.3% (14/42) of pSS patients having neurological manifestations as the initial symptom. The manifestations of hemiparesis (35.7%, 15/42), paraparesis (28.6%, 12/42), dysphonia (31.0%, 13/42), blurred vision (21.4%, 9/42), and dysfunctional proprioception (23.8%, 10/42) were more common in the pSS-CNS patients. Cerebral infarction (57.1%, 24/42), demyelination (31.0%, 13/42), myelitis (23.8%, 11/42), and angiostenosis (21.4%, 9/42) were most often found on MRI or CT scan imaging in the pSS-CNS patients. Intrathecal IgG level and total protein of cerebrospinal fluid were increased in 50% (8/16) of the pSS-CNS group. In comparison with patients without CNS involvement, the pSS-CNS patients were found to also have kidney and lung involvement, hematologic abnormalities, positive ANA and anti-SSA antibody tests, and reduced complement 3 (C3) and complement 4 (C4) levels (all p < 0.05). The prevalence of lung involvement, immune thrombocytopenia, and high-titer ANA (1:1000) were significantly higher in pSS-CNS disease activity compared to those in the moderately active group. Multivariate analysis identified lung involvement, anti-SSA positivity, and low C3 levels as prognostic factors for pSS-CNS. After high-dose glucocorticoids and immunosuppressive therapy, 60.5% (26/38) of pSS-CNS patients improved, 36.8% (14/38) were unresponsive to treatment, and 2.6% (1/38) died.

Conclusion: Clinical features are diverse in pSS-CNS patients, and the morbidity rate is low. CNS involvement was the initial presentation in state percentage here pSS patients. Pulmonary involvement, a positive anti-SSA antibody test, and reduced C3 levels are potential risk factors for CNS involvement in pSS. Treatment with high-dose glucocorticoids and immunosuppressive therapy appeared effective in 60% of pSS-CNS patients. Key Points • The CNS manifestations of pSS are diverse, and CNS imaging and CSF analysis are important for the diagnosis. • Pulmonary involvement, positive anti-SSA, and reduced C3 levels are potential risk factors of pSS-CNS. • About 60% of pSS-CNS patients were responsive to high-dose glucocorticoid administration and immunosuppressive therapy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873757PMC
http://dx.doi.org/10.1007/s10067-022-06448-wDOI Listing
February 2023

LncRNA DUXAP8 induces breast cancer radioresistance by modulating the PI3K/AKT/mTOR pathway and the EZH2-E-cadherin/RHOB pathway.

Authors:
Changjiang Lei Shaoting Li Ying Fan Li Hua Qingyun Pan Yuan Li Zhixiong Long Rui Yang

Cancer Biol Ther 2022 Dec;23(1):1-13

Department of General Surgery, the Fifth Hospital of Wuhan, Wuhan, China.

Radiation resistance poses a major clinical challenge in breast cancer (BC) treatment, but little is known about how long noncoding RNA (lncRNA) may regulate this phenomenon. Here, we reported that DUXAP8 was highly expressed in radioresistant BC tissues, and high expression of DUXAP8 was associated with poor prognosis. We found that the overexpression of DUXAP8 promoted radioresistance, while the knockdown of DUXAP8 expression increased radiosensitivity. Further studies revealed that DUXAP8 enhanced the radioresistance of BC cells by activating the PI3K/AKT/mTOR pathway and by repressing the expression of E-cadherin and RHOB through interaction with EZH2. Together, our work demonstrates that the overexpression of DUXAP8 promotes the resistance of BC cells toward radiation through modulating PI3K/AKT/mTOR pathway and EZH2-E-cadherin/RHOB axis. Targeting DUXAP8 may serve as a potential strategy to overcome radioresistance in BC treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635553PMC
http://dx.doi.org/10.1080/15384047.2022.2132008DOI Listing
December 2022

A New Product of Bilirubin Degradation by HO and Its Formation in Activated Neutrophils and in an Inflammatory Mouse Model.

Authors:
Fei-Fei Yu Yao Yuan Yan Ao Li Hua Wu Wang Yiyi Cao Jing Xi Yang Luan Shangwei Hou Xin-Yu Zhang

Biomolecules 2022 Sep 4;12(9). Epub 2022 Sep 4.

School of Public Health, Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Bilirubin (BR) is a tetrapyrrolic compound stemming from heme catabolism with diverse physiological functions. It can be oxidized by HO to form several degradation products, some of which have been detected in vivo and may contribute to the pathogenesis of certain diseases. However, the oxidative degradation of BR is complex and the conditions that BR degradation occurs pathophysiologically remain obscure. Neutrophils are known to generate large amounts of reactive oxygen species, including HO, upon activation and they are mobilized to inflammatory sites; therefore, we hypothesized that activated neutrophils could cause BR degradation, which could occur at inflammatory sites. In the present study, we investigated BR degradation by HO and identified hematinic acid (BHP1) and a new product BHP2, whose structure was characterized as 2,5-diformyl-4-methyl-1-pyrrole-3-propanoic acid. An LC-MS/MS method for the quantitation of the two compounds was then established. Using the LC-MS/MS method, we observed the concentration-dependent formation of BHP1 and BHP2 in mouse neutrophils incubated with 10 and 30 μM of BR with the yields being 16 ± 3.2 and 31 ± 5.9 pmol/10 cells for BHP1, and 25 ± 4.4 and 71 ± 26 pmol/10 cells for BHP2, respectively. After adding phorbol 12-myristate 13-acetate, a neutrophil agonist, to 30 μM of BR-treated cells, the BHP1 yield increased to 43 ± 6.6 pmol/10 cells, whereas the BHP2 one decreased to 47 ± 9.2 pmol/10 cells. The two products were also detected in hemorrhagic skins of mice with dermal inflammation and hemorrhage at levels of 4.5 ± 1.9 and 0.18 ± 0.10 nmol/g tissue, respectively, which were significantly higher than those in the non-hemorrhagic skins. BHP2 was neurotoxic starting at 0.10 μM but BHP1 was not, as assessed using as the animal model. Neutrophil-mediated BR degradation may be a universally pathophysiological process in inflammation and can be particularly important under pathological conditions concerning hemorrhage.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496627PMC
http://dx.doi.org/10.3390/biom12091237DOI Listing
September 2022

Analysis of the Construction of Big Data Platform in Scenic Spots to Increase the Number of Tourists: Taking Sports Group Performance as an Example.

Authors:
Lin Yu Li Hua Jiaran Ding

J Environ Public Health 2022 18;2022:3562778. Epub 2022 Aug 18.

Jiangxi University of Technology, Nanchang 330098, China.

The construction of a big data platform is the basis for improving the service level of scenic spots, and it is also a new media way to increase the number of tourists. At present, the scenic spot platform lacks effective evaluation methods and cannot analyze massive data, resulting in an insufficient increase in the number of tourists. Therefore, this paper analyzes the construction of the big data platform from the perspective of sports group performance, aiming at promoting the increase in the number of tourists in scenic spots. Firstly, the continuous clustering sampling method is used to make statistics on the massive tourist data in the platform. Secondly, the equidistant sampling coefficient is added to the sample data to ensure the validity of tourist data.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410818PMC
http://dx.doi.org/10.1155/2022/3562778DOI Listing
August 2022

Comparative analysis of arthroscopic-assisted Tight-rope technique and clavicular hook plate fixation in the treatment of Neer type IIB distal clavicle fractures.

Authors:
Si Nie Hong-Bo Li Li Hua Zhi-Ming Tang Min Lan

BMC Musculoskelet Disord 2022 Aug 6;23(1):756. Epub 2022 Aug 6.

Department of Orthopedics SurgeryDonghu DistrictJiangxi Province, Jiangxi Provincial People's Hospital, (The First Affiliated Hospital of Nanchang Medical College), 330006. No. 92 Aiguo Road, Nanchang, People's Republic of China.

Background: The aim of the present study was to compare the clinical efficacy of arthroscopic-assisted fixation using the Tight-rope system and clavicular hook plate fixation in the treatment of Neer IIB distal clavicle fractures.

Methods: We enrolled 48 consecutive patients with Neer IIB distal clavicle fractures who were treated at our institution from February 2016 and August 2020. These patients were divided into 2 groups based on the fixation method (16 cases with Tight-rope system and 32 cases with clavicular hook plate), and demographics and clinical characteristics of patients in different groups were compared.

Results: All 48 patients had functional outcome scores of the affected shoulder available at a mean of 23.8 ± 5.1 months, and there was a statistically significant improvement in the constant score, American shoulder and elbow surgeons (ASES) score, visual analogue scale (VAS) score at the end of follow-up (p < 0.001 respectively). However, the smaller length of skin incision, less estimated blood loss and shorter hospital stay were detected in the Tight-rope technique group patients than those of clavicular hook plate group patients (p < 0.001, respectively). Furthermore, the constant score, ASES score and VAS score were significantly improved in the Tight-rope technique group patients than those of clavicular hook plate group patients (p < 0.05, respectively).

Conclusions: Both Tight-rope technique and clavicular hook plate fixation can provide satisfactory clinical and radiological results in the treatment of distal clavicular Neer IIB fracture. However, arthroscopic-assisted fixation using the Tight-rope technique showed better results in terms of length of hospital stay, surgical trauma, clinical scores, and diagnose and treat concomitant glenohumeral pathologies.

Levels Of Evidence: III, Case-control study Retrospective comparative study.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356490PMC
http://dx.doi.org/10.1186/s12891-022-05724-9DOI Listing
August 2022

Pharmacokinetic Study of Nalbuphine in Surgical Patients Undergoing General Anesthesia with Varying Degrees of Liver Dysfunction.

Authors:
Xiao-Nan Gao Xu-Yang Nie Jing-Lin Gao Tian-Fang Heng Yu-Qi Zhang Li Hua Ya-Qi Sun Zhang-Ying Feng Ming-Xia Wang Li Jia

Drug Des Devel Ther 2022 26;16:2383-2393. Epub 2022 Jul 26.

Department of Anesthesiology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.

Purpose: This study aimed to characterize the pharmacokinetics of nalbuphine in patients undergoing general anesthesia with varying degrees of liver dysfunction.

Patients And Methods: Twenty-four patients were enrolled and divided into three cohorts based on liver function: normal liver function (n = 13), mild liver dysfunction (n = 5), and moderate/severe liver dysfunction (n = 6). During the induction of anesthesia, they received 15 mg of nalbuphine intravenously. Venous blood samples were collected from each patient. The plasma concentration of nalbuphine was determined using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The pharmacokinetic parameters of nalbuphine were calculated by non-compartmental analysis (NCA) using Phoenix WinNonlin software.

Results: Compared with the normal liver function group, the plasma elimination half-life (T) of nalbuphine was increased by approximately 33% in the moderate/severe liver dysfunction group (2.66 h vs 3.54 h, <0.05), and the volume of distribution (V) increased by approximately 85% (100.08 L vs 184.95 L, <0.05). Multivariate analysis revealed that weight and platelet were associated with clearance (CL); total bilirubin as an independent factor was associated with T, and weight associated with area under the curve (AUC) independently.

Conclusion: The T, mean residence time, and V of nalbuphine in patients with moderate/severe liver dysfunction were prolonged or increased significantly compared with those in the normal liver function group. These data suggest that it may need to be used with caution when nalbuphine is administered to patients with moderate or severe liver dysfunction.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341258PMC
http://dx.doi.org/10.2147/DDDT.S371596DOI Listing
August 2022

Analysis of the Influence of Sports on College Students' Music Vocalization Training Based on Monte Carlo and Dynamic Adjustment Factor Algorithm.

Authors:
Li Hua Lin Yu

J Environ Public Health 2022 16;2022:3443404. Epub 2022 Jul 16.

Jiangxi University of Technology, Nanchang 330098, China.

With the continuous improvement of people's living standards, the requirements of music majors for their training standards are also increasing, which leads to the development of music training in the direction of intelligence. This paper discusses the problems of breathing, coordination, and muscle control ability in vocal training and puts forward a vocal training method based on dynamic adjustment factor and Monte Carlo algorithm to solve the difficult problem of vocal training for college students and understand the relationship between vocal training and exercise. Firstly, the sports training set and vocal pronunciation training set are constructed in the form of clustering, and the samples in the set are analyzed discretely to ensure that the samples conform to normal distribution; then, using the Monte Carlo algorithm analyzes the two sample sets and finds out the relationship between exercise and vocal training. Finally, according to breathing, coordination, muscle control ability, and other indicators, calculate the impact of exercise on vocal sound. MATLAB simulation shows that the method proposed in this paper can analyze the influence of exercise on vocal vocalization from the perspective of breathing, coordination, muscle control ability, and other indicators. The accuracy of judgment results is more than 95%, and the time is less than 1 min. All indicators are better than traditional vocal training methods (90%, 2 min), which shows the effectiveness of the method proposed in this paper.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308556PMC
http://dx.doi.org/10.1155/2022/3443404DOI Listing
July 2022

APOA1 mRNA and protein in kidney renal clear cell carcinoma correlate with the disease outcome.

Authors:
Wei Zeng Guoguang Xiong Li Hua Yugang Hu Xufeng Guo Xiulan Peng

Sci Rep 2022 Jul 20;12(1):12406. Epub 2022 Jul 20.

Department of Oncology, The Second Affiliated Hospital of Jianghan University, 122 Xianzheng Road, Wuhan, 430050, Hubei Province, People's Republic of China.

Renal cancer is one of the most common malignant tumors with high mortality, and kidney renal clear cell carcinoma (KIRC) is the most common type of renal cancer. We attempted to evaluate the clinical and prognostic significance of Apolipoprotein A1 (APOA1) mRNA and protein in KIRC patients. Clinical data along with RNA-sequencing data were downloaded from UCSC Xena. The Human Protein Atlas database was searched to reveal APOA1 protein expression profiles in KIRC and normal renal tissues. The TIMER database was applied to determine the correlations of APOA1 with immune cells and PD-1 and PD-L1 in KIRC. Ninety-one cases of KIRC patients and 93 healthy controls from our hospital were enrolled for clinical validation. Levels of APOA1 mRNA in KIRC tissues (N = 535) are not only lower than the levels in normal renal tissues (N = 117), but also in paired normal renal tissues (N = 72). High expression of APOA1 mRNA at the time of surgery was correlated with worse overall survival (OS) (HR 1.66; p = 0.037) and disease-free survival (DFS) (HR 1.65; p = 0.047), and APOA1 DNA methylation was linked to worse OS (HR 2.1; p = 0.001) rather than DFS (HR 1.12; p = 0.624) in KIRC patients. Concentrations of preoperative serum APOA1 protein were markedly decreased in KIRC patients compared to healthy controls (p < 0.01), and low levels of APOA1 protein predicted less favorable OS than those with high levels (HR = 2.84, p = 0.0407). APOA1 negatively correlated with various immune cell infiltrates and PD-L1 expression (r = - 0.283, p = 2.74e-11) according to the TIMER database. Low levels of APOA1 mRNA at the time of surgery predict favorable survival in KIRC patients. Our results provide insights to identify a novel prognostic index with great clinical utility.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300670PMC
http://dx.doi.org/10.1038/s41598-022-16434-6DOI Listing
July 2022

Research on the Development Support Strategy of Cultural Enterprises Based on Fish Swarm Algorithm under the Background of Public Health.

Authors:
Lin Yu Li Hua Jiaran Ding

J Environ Public Health 2022 27;2022:6470147. Epub 2022 Jun 27.

Chengdu Jincheng College, Chengdu 611731, China.

Under the background of public health events, the government needs to adopt reasonable support strategies to help cultural enterprises tide over the crisis. However, the original analytic hierarchy process cannot be comprehensive and accurate analysis, resulting in that the government support strategy cannot play a role. Fish swarm algorithm is a comprehensive intelligent algorithm, which can comprehensively analyze the factors affecting the formulation of supporting strategies for cultural enterprises, and has the advantages of simple operation and strong analysis ability. Under this background, this paper puts forward a fish swarm model; this method is a comprehensive intelligent analysis method, which can help the government find the best support strategy from the perspectives of politics, economy, and society. In order to verify the effectiveness of the fish school model, this paper uses MATLAB software for verification; the results show that, in the support strategy classification, selection accuracy, and selection time, fish school model is superior to the analytic hierarchy process. Therefore, the fish swarm model can help the government to better choose supporting policies and help cultural enterprises tide over the crisis and improve the accuracy of formulating supporting strategies for cultural enterprises.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252644PMC
http://dx.doi.org/10.1155/2022/6470147DOI Listing
July 2022

Down-regulation of MLLT1 super elongation complex subunit impairs the anti-tumor activity of natural killer cells in esophageal cancer.

Authors:
Chong Liu Xueman Li Fei Xiong Lingying Wang Kang Chen Pingshang Wu Li Hua Zhuo Zhang

Immunobiology 2022 Jul 16;227(4):152238. Epub 2022 Jun 16.

The Department of Thoracic Surgery, Tongren Hospital of Wuhan University, 241 Pengliuyang Road, Wuchang District, Wuhan, Hubei Province 430060, China. Electronic address:

Natural killer (NK) cells actively participate in anti-tumor immunity and are thus regarded as a promising tool in immunotherapy against esophageal cancer (EC). However, the mechanisms regulating NK cell activation and exhaustion have not been completely elucidated. In this study, we characterized the expression and function of MLLT1 super elongation complex subunit (MLLT1) in esophageal NK cells in a mouse EC model. MLLT1 was down-regulated in esophageal NK cells, especially NK cells expressing both T cell immunoglobulin and mucin-domain containing-3 (TIM-3) and lymphocyte activation gene3(LAG-3). In vitro knockdown of MLLT1 in NK cells resulted in significant decreases in the expression of IFN-γ and perforin, as well as impaired NK cell cytotoxicity on tumor cells. Adoptive transfer of MLLT-deficient NK cells into EC-bearing mice showed consistent impairment of NK cell anti-tumor activity, as evidenced by decreases in IFN-γ and perforin but not granzyme B. Furthermore, EC tissue cells, which were enriched from the esophagus of EC-bearing mice, induced down-regulation of MLLT1 in splenic NK cells. This down-regulation was partially restored by a TIM-3 blocking antibody. Therefore, this study indicated that TIM-3 signaling down-regulated MLLT1 in esophageal NK cells, and MLLT1 down-regulation undermined the tumoricidal function of NK cells in EC. Our study unveils a novel mechanism underlying NK cell exhaustion/dysfunction in the EC microenvironment. MLLT1 could be a potential target in future NK cell-mediated immunotherapy against EC.
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http://dx.doi.org/10.1016/j.imbio.2022.152238DOI Listing
July 2022
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