Publications by authors named "Hua Guo"

1,072 Publications

  • Page 1 of 1

Reactive and Nonreactive Collisions between NO(XΠ) and O(P) under Hyperthermal Conditions.

J Phys Chem A 2022 Jun 24. Epub 2022 Jun 24.

Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, United States.

Quasiclassical trajectory calculations are performed for hyperthermal collisions between NO(XΠ) and O(P) on recently developed potential energy surfaces for the lowest doublet and quartet states of the NO system. Three product channels are investigated, and their branching fractions are in reasonably good agreement with the recent crossed molecular beam study at 84 kcal/mol of collision energy. The dominant inelastic channel has a strong forward scattering bias and a high translational energy distribution with limited internal excitation in the scattered NO. The exchange channel has significantly higher NO internal excitation and is also forward biased. The abstraction channel producing internally excited O has the smallest branching fraction and a broader angular distribution also with a forward peak. The angular and translational energy distributions in the three channels are consistent with experiment, but the agreement is not always quantitative. The sources of the differences are discussed. Finally, the impact of NO vibrational excitation on the reactive channels and the corresponding rate coefficients are reported.
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http://dx.doi.org/10.1021/acs.jpca.2c02735DOI Listing
June 2022

Frustrated Lewis Pairs in Heterogeneous Catalysis: Theoretical Insights.

Molecules 2022 Jun 10;27(12). Epub 2022 Jun 10.

Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, NM 87131, USA.

Frustrated Lewis pair (FLP) catalysts have attracted much recent interest because of their exceptional ability to activate small molecules in homogeneous catalysis. In the past ten years, this unique catalysis concept has been extended to heterogeneous catalysis, with much success. Herein, we review the recent theoretical advances in understanding FLP-based heterogeneous catalysis in several applications, including metal oxides, functionalized surfaces, and two-dimensional materials. A better understanding of the details of the catalytic mechanism can help in the experimental design of novel heterogeneous FLP catalysts.
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http://dx.doi.org/10.3390/molecules27123734DOI Listing
June 2022

Does Digital Inclusive Finance Effectively Promote Agricultural Green Development?-A Case Study of China.

Int J Environ Res Public Health 2022 Jun 7;19(12). Epub 2022 Jun 7.

College of Economics, Sichuan Agricultural University, Chengdu 611130, China.

Agricultural green development is increasingly being discussed in sustainable development. This paper constructs agricultural green development from four dimensions: resource savings, environmental protection, ecological conservation, and quality industrialization. We apply the entropy-weighted Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS) method to measure agricultural green development and employ a panel dataset of provinces in China from 2011-2019. Then, the dynamic spatial Durbin model is adopted to estimate the spatial effect of digital inclusive finance on agricultural green development. The main findings are as follows: (1) digital inclusive finance effectively promotes agricultural green development, and the promotional effect shows temporary and spatial spillover; (2) regional heterogeneity exists in the spatial effect in the short and long term; and (3) education, digital infrastructure, and traditional finance are important factors influencing this spatial effect of digital inclusive finance on agricultural green development.
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http://dx.doi.org/10.3390/ijerph19126982DOI Listing
June 2022

A single-cell atlas of tumor-infiltrating immune cells in pancreatic ductal adenocarcinoma.

Mol Cell Proteomics 2022 Jun 16:100258. Epub 2022 Jun 16.

School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. Electronic address:

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies with limited treatment options. To guide the design of more effective immunotherapy strategies, mass cytometry was employed to characterize the cellular composition of the PDAC-infiltrating immune cells. The expression of 33 protein markers was examined at the single-cell level in more than two million immune cells from four types of clinical samples, including PDAC tumors, normal pancreatic tissues, chronic pancreatitis tissues, and peripheral blood. Based on the analyses, we identified 23 distinct T cell phenotypes, with some cell clusters exhibiting aberrant frequencies in the tumors. PD-1 was extensively expressed in CD4 and CD8 T cells and co-expressed with both stimulatory and inhibitory immune markers. In addition, we observed elevated levels of functional markers, such as CD137L and CD69, in PDAC-infiltrating immune cells. Moreover, the combination of PD-1 and CD8 was used to stratify PDAC tumors from The Cancer Genome Atlas database into three immune subtypes, with S1 (PD-1CD8) exhibiting the best prognosis. Further analysis suggested distinct molecular mechanisms for immune exclusion in different subtypes. Taken together, the single-cell protein expression data depicted a detailed cell atlas of the PDAC-infiltrating immune cells and revealed clinically relevant information regarding useful cell phenotypes and targets for immunotherapy development.
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http://dx.doi.org/10.1016/j.mcpro.2022.100258DOI Listing
June 2022

Culturing Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) for Diagnosis and Genome Sequencing.

Lab Med 2022 Jun 17. Epub 2022 Jun 17.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Objective: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid detection "re-positive" phenomenon is encountered clinically. The accuracy of a viral nucleic acid test is crucial to prevent reintroduction of the virus into the community. This study evaluated the effect of virus culturing on increasing the sensitivity and specificity of real-time polymerase chain reaction (RT-PCR) detection and viral genomic sequencing.

Methods: A series of tenfold dilutions of a SARS-CoV-2 viral stock were conducted and cultured for either 24 or 48 hours. The viral load of cultured samples was determined by RT-PCR. The cultured and non-cultured samples of 1x 50% tissue culture infectious dose (TCID50) were sequenced using metagenomic next-generation sequencing. The depth and coverage of SARS-CoV-2 genome were measured.

Results: The lowest viral load detectable in a sample with RT-PCR was 0.01 TCID50. After a 24-h culture, the viral ORF 1ab and N-gene cycle threshold (CT) values were reduced by 4.4 points and 1 point, respectively. One TCID50 viral load of post 24-h culture revealed the sequence depth reached an average of 752 reads, compared with 0.15 in the nonculture; furthermore, the coverage was 99.99% while 6.42% in the nonculture.

Conclusion: These results indicate that virus culturing can significantly increase the viral load, which can increase the certainty of true-positive detection of the viral nucleic acids, and improve the quality of virus genomic sequencing.
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http://dx.doi.org/10.1093/labmed/lmac060DOI Listing
June 2022

Epigenetic Alterations of DNA Methylation and miRNA Contribution to Lung Adenocarcinoma.

Front Genet 2022 31;13:817552. Epub 2022 May 31.

Department of Thoracic Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, China.

This study focused on the epigenetic alterations of DNA methylation and miRNAs for lung adenocarcinoma (LUAD) diagnosis and treatment using bioinformatics analyses. DNA methylation data and mRNA and miRNA expression microarray data were obtained from The Cancer Genome Atlas (TCGA) database. The differentially methylated genes (DMGs), differentially expressed genes (DEGs), and differentially expressed miRNAs were analyzed by using the limma package. The DAVID database performed GO and KEGG pathway enrichment analyses. Using STRING and Cytoscape, we constructed the protein-protein interaction (PPI) network and achieved visualization. The online analysis tool CMap was used to identify potential small-molecule drugs for LUAD. In LUAD, 607 high miRNA-targeting downregulated genes and 925 low miRNA-targeting upregulated genes, as well as 284 hypermethylated low-expression genes and 315 hypomethylated high-expression genes, were obtained. They were mainly enriched in terms of pathways in cancer, neuroactive ligand-receptor interaction, cAMP signaling pathway, and cytosolic DNA-sensing pathway. In addition, 40 upregulated and 84 downregulated genes were regulated by both aberrant alternations of DNA methylation and miRNAs. Five small-molecule drugs were identified as a potential treatment for LUAD, and five hub genes (, , , , and ) were found in PPI, and two of them ( and ) may be related to the prognosis of LUAD. In summary, our study identified a series of differentially expressed genes associated with epigenetic alterations of DNA methylation and miRNA in LUAD. Five small-molecule drugs and five hub genes may be promising drugs and targets for LUAD treatment.
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http://dx.doi.org/10.3389/fgene.2022.817552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194831PMC
May 2022

Internal conversion and intersystem crossing dynamics based on coupled potential energy surfaces with full geometry-dependent spin-orbit and derivative couplings. Nonadiabatic photodissociation dynamics of NH(A) leading to the NH(XΣ, aΔ) + H channel.

Phys Chem Chem Phys 2022 Jun 22;24(24):15060-15067. Epub 2022 Jun 22.

Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA.

We simulate the photodissociation of NH originating from its first excited singlet state S into the NH + H (radical) and NH + H (molecular) channels. The states considered are the ground singlet state S, the first excited singlet state S and the lowest-lying triplet state T, which permit for the first time a uniform treatment of the internal conversion and intersystem crossing. The simulations are based on a diabatic potential energy matrix (DPEM) of S, S coupled by a conical intersection seam, as well as a potential energy surface (PES) for T coupled by spin-orbit coupling (SOC) to the two singlet states. The DPEM and PES are fitted to electronic structure data (ESD) including energies, energy gradients, and derivative couplings. The DPEM also defines an adiabatic to diabatic state (AtD) transformation, which is used to transform the singular adiabatic SOC into a smooth function of the nuclear coordinates in the diabatic representation, allowing the diabatic SOC to be fit to an analytical functional form. ESD and SOC data obtained from these surfaces can serve as input for either quantum or semi-classical characterization of the nonadiabatic dynamics. Using the SHARC suite of programs, nonadiabatic simulations based on over 40 000 semi-classical trajectories assess the convergence of our results. The production of NH + H is not direct, but is only achieved through a quasi-statistical dissociation mechanism after internal conversion to the ground electronic state. This leads to a much lower yield comparing with the main NH + H channel. The NH(XΣ) radical produced through the intersystem crossing from S to T is rare (∼0.2%) compared to NH(aΔ) due to the process being spin forbidden.
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http://dx.doi.org/10.1039/d2cp01271eDOI Listing
June 2022

Application of Neurite Orientation Dispersion and Density Imaging to Evaluate and Predict the Surgical Outcome for Degenerative Cervical Myelopathy.

Orthop Surg 2022 Jun 10. Epub 2022 Jun 10.

Department of Spine Surgery, Beijing Jishuitan Hospital, Beijing, China.

Objectives: Although the neurite orientation dispersion and density imaging (NODDI) has been shown useful to evaluate the spinal cord dysfunction, there are few prospective studies on analyzing the operation recovery of degenerative cervical myelopathy (DCM) disease using NODDI. This study aims to investigate the preoperative evaluation and predictive ability of NODDI in DCM patients who received posterior cervical laminoplasty.

Methods: This prospective study included 55 patients with DCM from January to December 2017. NODDI metrics, including intracellular volume fraction (Vic), isotropic volume fraction (Viso), and orientation dispersion index (ODI) were measured at the maximally compressed (MC) level and the non-compressed C2 level in each patient at the preoperative and the 3- and 6-month postoperative follow-up stages. Neurological function was assessed using the modified Japanese Orthopaedic Association (mJOA) scoring system at each stage. Spearman's correlation and Kendall's tau-b correlation were used to analyze the relationship between NODDI metrics and mJOA scores. Wilcoxon signed rank test was used to examine the changes in the NODDI and mJOA scores between the preoperative and 6-month follow-up stages. ROC analysis was used to further evaluate the predictive capability.

Results: Preoperative Vic at the level of C2 has a significant correlation with the preoperative mJOA score (r = 0.278, p = 0.048). Vic and Viso at the MC level were significantly different between the preoperative period and 6-month follow-up. Viso at the MC level was correlated with the mJOA score at 6-month follow-up (r = -0.302, p = 0.044). Vic and ODI at the C2 level predicted the surgical prognosis, with areas under the receiver-operating characteristic curve of 0.663 (p = 0.042) and 0.716 (p = 0.014).

Conclusions: The preoperative NODDI metrics at the C2 level are capable of evaluating the severity of spinal cord dysfunction and predict the surgical outcome.
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http://dx.doi.org/10.1111/os.13326DOI Listing
June 2022

Multi-echo balanced SSFP with a sequential phase-encoding order for functional MR imaging at 7T.

Magn Reson Med 2022 Jun 3. Epub 2022 Jun 3.

State Key Laboratory of Brain and Cognitive Science, Beijing MRI Center for Brain Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Purpose: To develop a 2D multi-echo passband balanced SSFP (bSSFP) sequence using an echo-train readout with a sequential phase-encoding order (sequential multi-echo bSSFP), and evaluate its performance in fast functional brain imaging at 7 T.

Methods: As images of sequential multi-echo bSSFP exhibit multiple ghosts due to periodic k-space modulations, a GRAPPA-based reconstruction method was proposed to eliminate ghosting artifacts. MRI experiments were performed to compare the image quality of multi-echo bSSFP and conventional single-echo bSSFP. Submillimeter-resolution fMRI using a checkerboard visual stimulus was conducted to compare the activation characteristics of multi-echo bSSFP, conventional single-echo bSSFP and standard gradient-echo EPI (GE-EPI).

Results: A higher mean structural similarity index was found between images of single-echo bSSFP and multi-echo bSSFP with a shorter echo train length (ETL). Multi-echo bSSFP (ETL = 3) showed higher temporal SNR (tSNR) values than GRAPPA-accelerated single-echo bSSFP (R = 2). In submillimeter-resolution fMRI experiments, multi-echo bSSFP (ETL = 3) approached the imaging speed of GRAPPA-accelerated single-echo bSSFP (R = 2), but without tSNR penalty and reduced activation due to acceleration. The median t-value and the number of significantly activated voxels were comparable between GE-EPI and multi-echo bSSFP (ETL = 3) that provides virtually distortion-free functional images and inherits the activation patterns of conventional bSSFP.

Conclusion: Sequential multi-echo bSSFP (ETL = 3) is suitable for fast fMRI with submillimeter in-plane resolution, and offers an option to accelerate bSSFP imaging without tSNR penalty like parallel imaging.
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http://dx.doi.org/10.1002/mrm.29301DOI Listing
June 2022

Full-dimensional quantum studies of vibrational energy transfer dynamics between HO and Ar: theory assessing experiment.

Phys Chem Chem Phys 2022 Jun 8;24(22):13542-13549. Epub 2022 Jun 8.

Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico, 87131, USA.

We report the first full-dimensional quantum mechanical calculations of the ro-vibrational inelastic scattering dynamics between water molecules and argon atoms on an accurate potential energy surface, using a recently developed time-independent quantum method based on the close-coupling approach. The state-to-state integral cross-sections and rate coefficients show strong observance of gap laws. The calculated thermal rate coefficients for the relaxation of the stretching fundamental states of HO are in good agreement with experimental values, while those for the bending overtone state are approximately five times smaller than the values extracted through a previous kinetic modeling of fluorescence decay data. Our state-specific quantum scattering results suggest the need to reassess the kinetic modeling of the experimental data. This work advanced our understanding of the quantum dynamics of vibrationally inelastic energy transfer processes involving polyatomic molecules.
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http://dx.doi.org/10.1039/d2cp01230hDOI Listing
June 2022

[Rapid screening of 84 pesticide residues in dendrobium by Sin-QuEChERS Nano purification column with gas chromatography-tandem mass spectrometry].

Se Pu 2022 Jun;40(6):565-575

Experimental Center, Guizhou Center for Disease Control and Prevention, Guiyang 550004, China.

A rapid screening method for 84 pesticide residues in dendrobium perfringens parent material with different polarities was developed using a Sin-QuEChERS Nano clean-up column combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). The differences in extraction efficiency of the targets were compared with different extraction solvents (acetonitrile containing 1% acetic acid, acetone) and methods (immersion with or without water). The purification effect and extraction recoveries of Sin-QuEChERS Nano method and classical dispersive solid-phase extraction (dSPE), solid-phase extraction (SPE) and QuEChERS were systematically compared using samples. The differences in matrix effects between the Sin-QuEChERS Nano method, which was more effective in purification, and the dSPE method were also analyzed. The purification effects of three commercially available Sin-QuEChERS Nano purification columns (simple matrix purification column, complex matrix purification column and herbal purification column) were compared. The applicability of the purification methods were also verified by using different parts of samples (stems, leaves and flowers). From the results, it could be concluded that weighing 2.00 g and the samples in 5 mL of water for 20 min, followed by extraction with acetonitrile containing 1% acetic acid was more effective. The average extraction recovery of the target components by Sin-QuEChERS Nano purification method was 90.5%, which further identified Sin-QuEChERS Nano-Chinese medicine purification column as the preferred purification column for dendrobium purification. The target components were separated by a DB-1701MS quartz capillary column (30 m×0.25 mm×0.25 μm) with programmed temperature rise, detected by multiple reaction monitoring (MRM) mode, and quantified by matrix-matched solution external standard method. The GC-MS/MS assay was used for the methodological validation of the 84 representative pesticides within and was carried out by GC-MS/MS detection method. The results indicated that the targets showed excellent linear correlation in different scopes with correlation coefficients () >0. 990. The limits of detection (LODs, =3) of the method were 1.5 to 5.8 μg/kg, and the limits of quantification (LOQs, =10) ranged from 5.0 to 15.0 μg/kg. The spiked recoveries of the target pesticides under different spiked levels were 68.7%-116.2%, and the relative standard deviations (RSDs, =6) were less than 15%. Compared to other typical pretreatment methods, the Sin-QuEChERS Nano method provided better performance in terms of purification. The method not only effectively removed pigments, organic acids, and alkaline interferents, but also saved preparation time. Losses due to solvent transfer were also avoided and no further vortexing or centrifugation was required, making it a simplified and effective extraction and purification procedure. The method was sensitive, rapid, simple and reliable. It effectively improved the detection efficiency during the rapid screening of pesticides in dendrobium and presented a strong practical application value. In addition, the developed method could further expand the types of target pesticides and could be used to detect more pesticide residues in foods and Chinese herbal medicine. The established Sin-QuEChERS Nano method was used for the analysis of authentic samples. The applicability of the method was evaluated by analyzing a total of 80 samples collected from Anlong, Libo, Dushan, and Yanhe County in Guizhou Province. The types of samples included dendrobium maple, (flowers, stems, leaves) and (flowers, stems, leaves, powder, tablets). At least one pesticide residue was detected in 12 samples, with a detection rate of 15%. The five pesticides with higher detection rates and residues were chlorpyrifos (0.08-0.5 mg/kg), chlorothalonil (0.06-3.2 mg/kg), propanil zinc (0.03-0.15 mg/kg), methyl parathion (0.04-0.23 mg/kg) and cyhalothrin (0.10-2.68 mg/kg). Except for the pesticides in maximum residue limits (MRLs), the pesticide residues detected from dendrobium samples were below the limits set by Chinese national standard (GB 2763-2021) and local standard DBS 52/048-2020.
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http://dx.doi.org/10.3724/SP.J.1123.2021.12010DOI Listing
June 2022

Tumor Immune Microenvironment and Response to Neoadjuvant Chemotherapy in Hormone Receptor/HER2+ Early Stage Breast Cancer.

Clin Breast Cancer 2022 Apr 18. Epub 2022 Apr 18.

Division of Radiation Oncology, Columbia University Irving Medical Center, New York, NY. Electronic address:

Background: Pathologic response at the time of surgery after neoadjuvant therapy for HER2 positive early breast cancer impacts both prognosis and subsequent adjuvant therapy. Comprehensive descriptions of the tumor microenvironment (TME) in patients with HER2 positive early breast cancer is not well described. We utilized standard stromal pathologist-assessed tumor infiltrating lymphocyte (TIL) quantification, quantitative multiplex immunofluorescence, and RNA-based gene pathway signatures to assess pretreatment TME characteristics associated pathologic complete response in patients with hormone receptor positive, HER2 positive early breast cancer treated in the neoadjuvant setting.

Methods: We utilized standard stromal pathologist-assessed TIL quantification, quantitative multiplex immunofluorescence, and RNA-based gene pathway signatures to assess pretreatment TME characteristics associated pathologic complete response in 28 patients with hormone receptor positive, HER2 positive early breast cancer treated in the neoadjuvant setting.

Results: Pathologist-assessed stromal TILs were significantly associated with pathologic complete response (pCR). By quantitative multiplex immunofluorescence, univariate analysis revealed significant increases in CD3+, CD3+CD8-FOXP3-, CD8+ and FOXP3+ T-cell densities as well as increased immune cell aggregates in pCR patients. In subsets of paired pre/post-treatment samples, we observed significant changes in gene expression signatures in non-pCR patients and significant decreases in CD8+ densities after treatment in pCR patients. No RNA based pathway signature was associated with pCR.

Conclusion: TME characterization HER2 positive breast cancer patients revealed several stromal T-cell densities and immune cell aggregates associated with pCR. These results demonstrate the feasibility of these novel methods in TME evaluation and contribute to ongoing investigations of the TME in HER2+ early breast cancer to identify robust biomarkers to best identify patients eligible for systemic de-escalation strategies.
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http://dx.doi.org/10.1016/j.clbc.2022.04.002DOI Listing
April 2022

DNAJC24 is a potential therapeutic target in hepatocellular carcinoma through affecting ammonia metabolism.

Cell Death Dis 2022 May 24;13(5):490. Epub 2022 May 24.

Department of Tumor Cell Biology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China.

Evolutionarily conserved heat shock proteins are involved in the heat shock response of cells in response to changes in the external environment. In normal tissues, heat shock proteins can help cells survive in a rapidly changing environment. Likewise, in malignant tumors heat shock proteins may help tumor cells cope with external stresses as well as the stress of treatment. In this way they become accomplices of malignant tumors. Here we demonstrated for the first time that high expression of DNAJC24 (a heat shock protein) shortens survival in patients with HCC by immunohistochemical staining of 167 paired hepatocellular carcinomas and paraneoplastic tissues as well as data from public databases. In vitro experiments demonstrated that stimuli such as hypoxia, starvation and heat could upregulate DNAJC24 expression in HCC cells through transcriptional regulation of HSF2, and high expression of DNAJC24 in HCC cells could promote the proliferation and motility of HCC cells. In addition, we also verified that targeting DNAJC24 under normal culture conditions can affect the proliferation and autophagy of HCC cells by interfering with ammonia metabolism, thereby inhibiting the malignant progression of HCC. Overall, we suggested that DNAJC24 may become a new target for the treatment of HCC.
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http://dx.doi.org/10.1038/s41419-022-04953-zDOI Listing
May 2022

Differential Cross Sections for Cold, State-to-State Spin-Orbit Changing Collisions of NO( = 10) with Neon.

J Phys Chem A 2022 Jun 23;126(21):3338-3346. Epub 2022 May 23.

Department of Chemistry, University of Missouri, Columbia, Missouri 65211, United States.

Inelastic scattering processes have proven a powerful means of investigating molecular interactions, and much current effort is focused on the cold and ultracold regime where quantum phenomena are clearly manifested. Studies of collisions of the open shell nitric oxide (NO) molecule have been central in this effort since the pioneering work of Houston and co-workers in the early 1990s. State-to-state scattering of vibrationally excited molecules in the cold regime introduces challenges that test the suitability of current theoretical methods for determination of intermolecular potentials, and concomitant electronically nonadiabatic processes raise the bar further. Here we report measurements of differential cross sections for state-to-state spin-orbit changing collisions of NO ( = 10, Ω″ = 1.5, and ″ = 1.5) with neon from 2.3 to 3.5 cm collision energy using our recently developed near-copropagating beam technique. The experimental results are compared with those obtained from quantum scattering calculations on a high-level set of coupled cluster potential energy surfaces and are shown to be in good agreement. The theoretical results suggest that distinct backscattering in the 2.3 cm case arises from overlapping resonances.
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http://dx.doi.org/10.1021/acs.jpca.2c02698DOI Listing
June 2022

White matter regeneration induced by aligned fibrin nanofiber hydrogel contributes to motor functional recovery in canine T12 spinal cord injury.

Regen Biomater 2022 29;9:rbab069. Epub 2021 Nov 29.

State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

A hierarchically aligned fibrin hydrogel (AFG) that possesses soft stiffness and aligned nanofiber structure has been successfully proven to facilitate neuroregeneration and . However, its potential in promoting nerve regeneration in large animal models that is critical for clinical translation has not been sufficiently specified. Here, the effects of AFG on directing neuroregeneration in canine hemisected T12 spinal cord injuries were explored. Histologically obvious white matter regeneration consisting of a large area of consecutive, compact and aligned nerve fibers is induced by AFG, leading to a significant motor functional restoration. The canines with AFG implantation start to stand well with their defective legs from 3 to 4 weeks postoperatively and even effortlessly climb the steps from 7 to 8 weeks. Moreover, high-resolution multi-shot diffusion tensor imaging illustrates the spatiotemporal dynamics of nerve regeneration rapidly crossing the lesion within 4 weeks in the AFG group. Our findings indicate that AFG could be a potential therapeutic vehicle for spinal cord injury by inducing rapid white matter regeneration and restoring locomotion, pointing out its promising prospect in clinic practice.
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http://dx.doi.org/10.1093/rb/rbab069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089163PMC
November 2021

High efficiency of left superior frontal gyrus and the symptom features of major depressive disorder.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2022 Mar;47(3):289-300

Department of Psychiatry, Second Xiangya Hospital, Central South University, Changsha 410011.

Objectives: Major depressive disorder (MDD) patients with anhedonia tend to have a poor prognosis. The underlying imaging basis for anhedonia in MDD remains largely unknown. The relationship between nodal properties and anhedonia in MDD patients need to be further investigated. Herein, this study aims to explore differences of cerebral functional node characteristics in MDD patients with severe anhedonia (MDD-SA) and MDD patients with mild anhedonia (MDD-MA) before and after the antidepressant treatment.

Methods: Ninety participants with current MDD were recruited in this study. 24-Item Hamilton Depression Scale (HAMD-24) and Snaith-Hamilton Pleasure Scale (SHAPS) were used to assess the severity of depression and anhedonia at baseline and the end of 6-months treatment. The MDD patients who scored above the 25th percentile on the SHAPS were assigned to an MDD-SA group (19), while those who scored below the 25th percentile were assigned to an MDD-MA group (18). All patients in the 2 groups received antidepressant treatment. Functional magnetic resonance imaging (fMRI) images of all the patients were collected at baseline and the end of 6-months treatment. Graph theory was applied to analyze the patients' cerebral functional nodal characteristics, which were measured by efficiency (e) and degree (k).

Results: Repeated measures 2-factor ANCOVA showed significant main effects on group on the e and k values of left superior frontal gyrus (LSFG) (0.003 and 0.008, respectively), and on the e and k values of left medial orbital-frontal gyrus (LMOFG) (=0.004 and 0.008, respectively). Compared with the MDD-MA group, the significantly higher e and k values of the LSFG (0.015 and 0.021, respectively), and the significantly higher e and k values of the LMOFG (=0.015 and 0.037, respectively) were observed in the MDD-SA group at baseline. Meanwhile, higher SHAPS scores could result in higher e and k values of LSFG (=0.019 and 0.026, respectively), and higher e value of LMOFG (0.040) at baseline; higher SHAPS scores could result in higher e values of LSFG (0.049) at the end of 6-months treatment. The multiple linear regression analysis revealed that sex were negatively correlated with the e and k values of LSFG (= -0.014, =0.004; =-1.153, =0.001, respectively). The onset age of MDD was negatively correlated with the k value of LSFG (=-0.420, =0.034) at the end of 6-months treatment. We also found that SHAPS scores at baseline were positively correlated with the HAMD-24 scores (=0.387, =0.022) at the end of 6-months treatment.

Conclusions: There are obvious differences in nodal properties between the MDD-SA and the MDD-MA patients, such as the high e of LSFG in the MDD-SA patients, which may be associated with the severity of anhedonia. These nodal properties could be potential biomarkers for the prognosis of MDD. The increased e and k values in the LSFG of MDD-SA patients may underlie a compensatory mechanism or protective mechanism. The mechanism may be an important component of the pathological mechanism of MDD-SA. The poor prognosis in the MDD-SA patients suggests that anhedonia may predict a worse prognosis in MDD patients. Sex and onset age of MDD may affect the nodal properties of LSFG at baseline and the end of 6-months treatment.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2022.210743DOI Listing
March 2022

Acetylene hydrogenation catalyzed by bare and Ni doped CeO(110): the role of frustrated Lewis pairs.

Phys Chem Chem Phys 2022 May 11;24(18):11295-11304. Epub 2022 May 11.

Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, USA.

Ceria (CeO) has recently been found to catalyze the selective hydrogenation of alkynes, which has stimulated much discussion on the catalytic mechanism on various facets of reducible oxides. In this work, H dissociation and acetylene hydrogenation on bare and Ni doped CeO(110) surfaces are investigated using density functional theory (DFT). Similar to that on the CeO(111) surface, our results suggest that catalysis is facilitated by frustrated Lewis pairs (FLPs) formed by oxygen vacancies (Os) on the oxide surfaces. On bare CeO(110) with a single O (CeO(110)-O), two surface Ce cations with one non-adjacent O anion are shown to form (Ce-Ce)/O quasi-FLPs, while for the Ni doped CeO(110) surface with one (Ni-CeO(110)-O) or two (Ni-CeO(110)-2O) Os, one Ce and a non-adjacent O counterions are found to form a mono-Ce/O FLP. DFT calculations indicate that Ce/O FLPs facilitate the H dissociation a heterolytic mechanism, while the resulting surface O-H and Ce-H species catalyze the subsequent acetylene hydrogenation. With CeO(110)-O and Ni-CeO(110)-2O, our DFT calculations suggest that the first hydrogenation step is the rate-determining step with a barrier of 0.43 and 0.40 eV, respectively. For Ni-CeO(110)-O, the reaction is shown to be controlled by the H dissociation with a barrier of 0.41 eV. These barriers are significantly lower than that (about 0.7 eV) on CeO(111), explaining the experimentally observed higher catalytic efficiency of the (110) facet of ceria. The change of the rate-determining step is attributed to the different electronic properties of Ce in the Ce/O FLPs - the Ce f states closer to the Fermi level not only facilitate the heterolytic dissociation of H but also lead to a higher barrier of acetylene hydrogenation.
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http://dx.doi.org/10.1039/d2cp00925kDOI Listing
May 2022

Role of Necroptosis and Immune Infiltration in Human Stanford Type A Aortic Dissection: Novel Insights from Bioinformatics Analyses.

Oxid Med Cell Longev 2022 16;2022:6184802. Epub 2022 Apr 16.

Department of Cardiology, Shaanxi Provincial People's Hospital, Xi'an, China.

Background: Stanford type A aortic dissection (TAAD) is one of the most life-threatening cardiovascular emergencies with high mortality and morbidity, and necroptosis is a newly identified type of programmed cell death and contributes to the pathogenesis of various cardiovascular diseases. However, the role of necroptosis in TAAD has not been elucidated. This study was aimed at determining the role of necroptosis in TAAD using bioinformatics analyses.

Methods: The RNA sequencing dataset GSE153434 and the microarray dataset GSE52093 were obtained from Gene Expression Omnibus (GEO) database. Differentially expressed genes of necroptosis (NRDEGs) were identified based on differentially expressed genes (DEGs) and necroptosis gene set. Gene set enrichment analysis (GSEA) was applied to evaluate the gene enrichment signaling pathway in TAAD. The STRING database and Cytoscape software were used to establish and visualize protein-protein interaction (PPI) networks and identify the key functional modules of NRDEGs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of NRDEGs were also performed. Additionally, Spearman correlations were used to construct the necroptosis-related transcription factor-target genes regulatory network, immune infiltration patterns were analyzed using the ImmuCellAI algorithm, and the correlation between immune cell-type abundance and NRDEGs expression was investigated. The expression levels of NRDEGs and immune infiltration were additionally verified in the GSE52093 dataset.

Results: We found that the necroptosis pathway was considerably enriched and activated in TAAD samples. Overall, 25 NRDEGs were identified including MLKL, RIPK1, and FADD, and among them, 18 were verified in the validation set. Moreover, GO and KEGG enrichment analyses found that NRDEGs were primarily involved in the tumor necrosis factor signaling pathway, nucleotide-binding oligomerization domain-like receptor signaling pathway, and interleukin-17 signaling pathway. The imbalance of Th17/Treg cells was identified in the TAAD samples. Furthermore, correlation analysis indicated that expression of NRDEGs was positively associated with proinflammatory immune-cell infiltrations and negatively associated with anti-inflammatory or regulatory immune-cell infiltrations.

Conclusions: The present findings suggest that necroptosis phenomenon exists in TAAD and correlates with immune cell infiltration, which indicate necroptosis may promote the development of TAAD through activating immune infiltration and immune response. This study paves a new road to future investigation of the pathogenic mechanisms and therapeutic strategies for TAAD.
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http://dx.doi.org/10.1155/2022/6184802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036163PMC
April 2022

NRIP1 aggravates lung injury caused by in mice by increasing PIAS1 ubiquitination.

Aging (Albany NY) 2022 04 23;14(8):3529-3539. Epub 2022 Apr 23.

Department of Microbiology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an 710061, China.

Recently, evidence has shown that nuclear receptor interacting protein 1 (NRIP1) is involved in acute lung injury (ALI) progression, but the specific mechanism remains unclear. (PA)-treated TC-1 cells were transfected with pcDNA-NRIP1 or si-NRIP1, and we found that overexpression of NRIP1 inhibited cell viability and promoted cell apoptosis and secretion of inflammatory factors, and transfection of si-NRIP1 reversed these effects. Furthermore, online bioinformatics analysis and co-immunoprecipitation assay results indicated that NRIP1 could bind to Ubiquitin Conjugating Enzyme E2I (UBE2I), and promoted UBE2I expression. Next, the PA-treated TC-1 cells were transfected with si-NRIP1 alone or together with pcDNA-UBE2I, and we observed that transfection with si-NRIP1 inhibited UBE2I expression, promoted cell viability, and reduced cell apoptosis and inflammatory factor secretion, which could be reversed by UBE2I overexpression. Moreover, UBE2I could bind to protein inhibitor of activated signal transducer and activators of transcription 1 (PIAS1). Overexpression of NRIP1 promoted UBE2I expression and inhibited PIAS1 expression, and NRIP1 promoted PIAS1 ubiquitination and degradation by UBE2I. The PA-treated TC-1 cells were transfected with si-UBE2I alone or together with si-PIAS1, and the results indicated that transfection of si-UBE2I had the same effect as transfection of si-NRIP1. Finally, our findings indicated that the expression of NRIP1 and UBE2I was decreased, and PIAS1 expression was increased, in the lung tissues of mice with NRIP1 knocked-down, and the inflammatory infiltration in the lung tissue was reduced. In conclusion, our study demonstrates that NRIP1 aggravates PA-induced lung injury in mice by promoting PIAS1 ubiquitination.
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http://dx.doi.org/10.18632/aging.204027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085225PMC
April 2022

Analysis of clinical research publication patterns to characterize the cooperation among China's "double first-class" construction medical universities: based on social network analysis.

Authors:
Xue Jing Guo Hua

Ann Transl Med 2022 Mar;10(6):320

Research Department, Hospital Management Institute, Xiangya Hospital, Central South University, Changsha, China.

Background: The "Double First-Class" refers to world first-class university and first-class academic discipline construction proposed by China government. The "Double First-Class" construction medical universities have made many clinical research achievements, but the analysis and evaluation of research collaboration networks in the field of clinical research are still lacking.

Methods: Clinical research papers by 23 "Double First-Class" construction medical universities in China from 2000 to 2019 indexed in the databases of the China National Knowledge Infrastructure and Web of Science (WoS) databases were collected. Through the social network analysis (SNA) method and Ucinet software, the interuniversity research cooperation networks of domestic and international publications were comparatively analyzed at the overall, individual, and group levels in terms of number of publications, cooperation network matrix, centrality analysis, cohesive subgroup analysis, and core-periphery structure to understand the developmental status of the cooperative network of clinical research publications among China's "Double First-Class" construction medical universities.

Results: The cooperation among China's "Double First-Class" construction universities showed certain regional distribution characteristics, and they showed closer cooperation in publishing papers in international journals than in domestic ones. The overall density of the domestic-journal research collaboration network of the universities was 0.4229, mainly centered on Beijing University of Traditional Chinese Medicine and Shanghai University of Traditional Chinese Medicine. In contrast, the overall density of international-journal research collaboration network was 0.9052, mainly centered on Peking University, Central South University, and Zhengzhou University, with large differences in subgroup density and low integration.

Conclusions: To promote the development of clinical research, it is necessary to improve the construction of the interuniversity clinical research collaboration system, build a national clinical research network with a multilevel structure and sophisticated functions, and expand resource sharing as well as collaborative innovation capacity.
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http://dx.doi.org/10.21037/atm-22-516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011218PMC
March 2022

Effects of Jie Yu Wan on Generalized Anxiety Disorder: A Randomized Clinical Trial.

Evid Based Complement Alternat Med 2022 8;2022:9951693. Epub 2022 Apr 8.

The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders & Beijing Anding Hospital, Capital Medical University, Beijing 100088, China.

Objective: To systematically assess the clinical efficacy of the (JYW) formula in treating generalized anxiety disorder (GAD).

Methods: A multicenter, prospective, double-blind, double-dummy, randomized controlled trial (RCT) was conducted at four hospitals in China. A total of one hundred thirty-three patients with GAD were enrolled from 2017 to 2019. This study aimed to evaluate the effects of a Traditional Chinese Medicine (TCM) JYW formula on GAD at eight weeks, with the use of Buspirone as the comparator. A stepwise dosing protocol was used (JYW: high dose 24 g/day, low dose 12 g/day; Buspirone: high dose 30 mg/day, low dose 15 mg/day) and the dose was adjusted depending on whether the treatment response of Hamilton Anxiety Scale (HAMA) score was less than or equal to 25% after one week. The primary outcome was a change in total score on the HAMA. The secondary outcomes included the Hamilton Depression Scale (HAMD), Clinical Global Impression (CGI) scale, and TCM Syndrome Scale. Adverse events were recorded using the Treatment Emergent Symptom Scale (TESS). Assessments were conducted at the baseline and 1, 2, 4, and 8 weeks.

Results: A total of one hundred thirty-three participants were randomly assigned to the JYW group ( = 66) and the Buspirone group ( = 67). One hundred twenty-one patients (91%) completed at least one follow-up session. There were no significant differences between the two groups in terms of gender, age, disease course, HAMA, HAMD, CGI, and TCM Syndrome Scale scores at baseline (all > 0.05). Repeated-measures analysis of variance revealed statistically significant time effects for the HAMA (=0.002), HAMD ( = 0.018), and CGI (=0.001) in both groups. Sensitivity analyses supported the credibility of the main results ( > 0.05). The group effect was not significant for the HAMA (=0.43), HAMD (=0.27), CGI (=0.37), and TCM Syndrome Scale (=0.86). Furthermore, there were no significant interaction effects between time and group in terms of the HAMA (=0.47), HAMD (=0.79), CGI (=0.67), and TCM Syndrome Scale (=0.69). After one week, 53 patients (80%) of the JYW group and 52 patients (78%) of the Buspirone group were adjusted to high doses. The interaction effect between time, group, and the dose was determined by repeated measures ANOVA test, and the HAMA score served as the outcome measure. The interaction effect between time and dose was statistically significant (=0.04), which shows that high-dose JYW (24 g/day) was more effective in decreasing patients' HAMA scores than low-dose JYW (12 g/day), and Buspirone had the same effect, which means that high-dose Buspirone (30 mg/day) was more effective than low dose. (15 mg/day).

Conclusions: The conclusion of this study supports that JYW and Buspirone can effectively alleviate the anxiety symptoms of GAD patients, which are both effective and safe for treatment of mild to moderate GAD. Besides, high-dose JYW or Buspirone are more effective than low-dose, which is of great importance in assisting clinical medication choice.
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http://dx.doi.org/10.1155/2022/9951693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9012658PMC
April 2022

Development of Mini-Barcode Based on Chloroplast Genome and Its Application in Metabarcoding Molecular Identification of Chinese Medicinal Material Radix (Chishao).

Front Plant Sci 2022 31;13:819822. Epub 2022 Mar 31.

State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Radix (Chishao), a typical multi-origin Chinese medicinal material, originates from the dried roots of or . The previous study suggested that these two commonly used Chishao showed variation in their chemical compositions and clinical efficacies. Therefore, accurate identification of different Chishao species was of great significance for the guide of clinical medication, and timely treatment of patients. In this study, the chloroplast genome sequences of and were obtained by next-generation sequencing (NGS) technology, and then the hypervariable regions were selected to design two mini-barcode candidates for species identification. Combined with DNA metabarcoding technology, we performed qualitative and quantitative analysis on the artificially mixed samples of and and evaluated the identification ability of these mini-barcode candidates. Furtherly, the mini-barcode with good performance was applied to distinguish the Chinese patent medicine "cerebral thrombosis tablets" containing Chishao. The results indicated that the chloroplast genomes of and were 152,750 and 152,527 bp, respectively. As published previously, they exhibited a typical quadripartite structure including a large single-copy region (LSC), a small single-copy region (SSC) and a pair of inverted repeat regions (IRs). The nucleotide polymorphism analysis revealed seven variable protein-coding regions as , , , , , , and , and two mini-barcodes were developed from and respectively. The result suggested that both two mini-barcodes performed well distinguishing from . Besides, was the only raw material of Chishao in all collected "cerebral thrombosis tablets" samples. In general, this study has established a method to realize the qualitative and quantitative identification of Chishao as multi-origin Chinese medicinal materials, which can be applied to Chinese patent medicines containing Chishao.
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http://dx.doi.org/10.3389/fpls.2022.819822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009180PMC
March 2022

Sulfated alginate oligosaccharide exerts antitumor activity and autophagy induction by inactivating MEK1/ERK/mTOR signaling in a KSR1-dependent manner in osteosarcoma.

Oncogenesis 2022 Apr 13;11(1):16. Epub 2022 Apr 13.

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 200233, Shanghai, China.

Alginate oligosaccharide (AOS) has the function to inhibit tumor progression and the sulfated modification can enhance the antitumor activity. To date, the function and mechanism of sulfated AOS (AOS-SO) in tumors remain largely elusive. We prepared AOS by the enzymatic degradation of alginate, collected AOS-SO by sulfating following the canonical procedure. Using these materials, in vitro assays showed that both AOS and AOS-SO elicited antitumor effects in osteosarcoma cells. Sulfated modification significantly enhanced the antitumor activity. In addition, AOS-SO had obvious effects on cell cycle arrest, apoptosis, and autophagy induction in vitro and in vivo. Mechanistically, we observed that AOS-SO treatment triggered proapoptotic autophagy by inhibiting MEK1/ERK/mTOR signaling. The ERK activator reversed AOS-SO-induced autophagy. More importantly, we found that KSR1 interacted with MEK1 and functioned as a positive regulator of MEK1 protein in osteosarcoma cells. High KSR1 expression was significantly associated with poor survival in osteosarcoma patients. Together, these results suggest that AOS-SO has a better antitumor effect in osteosarcoma by inhibiting MEK1/ERK/mTOR signaling, which is KSR1-dependent; thus, AOS-SO can be a new potential therapeutic candidate for the treatment of osteosarcoma.
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http://dx.doi.org/10.1038/s41389-022-00390-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008062PMC
April 2022

First-Principles Insights into Adiabatic and Nonadiabatic Vibrational Energy-Transfer Dynamics during Molecular Scattering from Metal Surfaces: The Importance of Surface Reactivity.

J Phys Chem Lett 2022 Apr 12;13(15):3450-3461. Epub 2022 Apr 12.

Department of Chemical Physics, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui 230026, China.

Energy transfer is ubiquitous during molecular collisions and reactions at gas-surface interfaces. Of particular importance is vibrational energy transfer because of its relevance to bond forming and breaking. In this Perspective, we review recent first-principles studies on vibrational energy-transfer dynamics during molecular scattering from metal surfaces at the state-to-state level. Taking several representative systems as examples, we highlight the intrinsic correlation between vibrational energy transfer in nonreactive scattering and surface reactivity and how it operates in both electronically adiabatic and nonadiabatic pathways. Adiabatically, the presence of a dissociation barrier softens a bond in the impinging molecule and increases its couplings with other molecular modes and surface phonons. In the meantime, the stronger interaction between the molecule and the surface also changes the electronic structure at the barrier, resulting in an increase of nonadiabatic effects. We further discuss future prospects toward a more quantitative understanding of this important surface dynamical process.
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http://dx.doi.org/10.1021/acs.jpclett.2c00593DOI Listing
April 2022

Eliminating the original cargos of glioblastoma cell-derived small extracellular vesicles for efficient drug delivery to glioblastoma with improved biosafety.

Bioact Mater 2022 Oct 16;16:204-217. Epub 2022 Mar 16.

Department of Neurosurgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai, China.

Tumor derived small extracellular vesicles (TsEVs) display a great potential as efficient nanocarriers for chemotherapy because of their intrinsic targeting ability. However, the inherited risks of their original cargos (like loaded proteins or RNAs) from parent cancer cells in tumor progression severely hinder the practical application. In this study, a saponin-mediated cargo elimination strategy was established and practiced in glioblastoma (GBM) cell-derived small extracellular vesicles (GBM-sEVs). A high eliminating efficacy of the cargo molecules was confirmed by systematic analysis of the original proteins and RNAs in GBM-sEVs. In addition, the inherited functions of GBM-sEVs to promote GBM progression vanished after saponin treatment. Moreover, the results of cellular uptake analysis and imaging analysis demonstrated that saponin treatment preserved the homotypic targeting ability of GBM-sEVs. Thus, we developed an efficient nanocarrier with improved biosafety for GBM suppression. Furthermore, doxorubicin (DOX) transported by the saponin-treated GBM-sEVs (sa-GBM-sEVs) displayed an effective tumor suppression in both subcutaneous and orthotopic GBM models of mouse. Collectively, this study provides a feasible way to avoid the potential protumoral risks of TsEVs and can advance the clinical application of TsEVs in chemotherapy.
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http://dx.doi.org/10.1016/j.bioactmat.2022.02.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965721PMC
October 2022

General orientation transform for the estimation of fiber orientations in white matter tissues.

Magn Reson Med 2022 Aug 5;88(2):945-961. Epub 2022 Apr 5.

Center for Nano & Micro Mechanics, Department of Engineering Mechanics, Tsinghua University, Beijing, China.

Purpose: The orientation distribution function (ODF), which is obtained from the radial integral of the probability density function weighted by ( is the radial length), has been used to estimate fiber orientations of white matter tissues. Currently, there is no general expression of the ODF that is suitable for any n value in the HARDI methods.

Theory And Methods: A novel methodology is proposed to calculate the ODF for any through the Taylor series expansion and a generalized expression for is provided. Then a series of single-shell HARDI methods, termed the general orientation transform (GOT), is developed based on the obtained expression. By combining complementary GOTs, a composite estimator is obtained and further optimized via constrained optimization to take full advantage of individual merits. The final optimized HARDI method is termed the combined GOT with constrained optimization (coGOT). The proposed method is compared with other commonly used HARDI methods on the simulated data, the physical phantom data, the ISMRM 2015 Tractography challenge data, and in vivo HCP datasets.

Results: coGOT can resolve crossing fibers with higher resolution, performs better robustness, generates fewer spurious lobes in glyphs, and thus provides distinct improvement in the tractography. The evaluations show coGOT's superior capability in reconstructing the fiber orientations from dMRI signals.

Conclusions: Generalization of the ODF allows us to obtain a wide range of HARDI estimators to select suitable candidates for composite formulation. The optimized estimator coGOT has great potential for studying neural architecture and serving as fiber tracking tools.
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http://dx.doi.org/10.1002/mrm.29256DOI Listing
August 2022

Establish of an Initial Platinum-Resistance Predictor in High-Grade Serous Ovarian Cancer Patients Regardless of Homologous Recombination Deficiency Status.

Front Oncol 2022 18;12:847085. Epub 2022 Mar 18.

Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.

Backgrounds: Ovarian cancer (OC) is still the leading aggressive and lethal disease of gynecological cancers, and platinum-based regimes are the standard treatments. However, nearly 20%-30% of patients with OC are initial platinum resistant (IPR), and there is a lack of valid tools to predict whether they will be primary platinum resistant or not prior to chemotherapy.

Methods: Transcriptome data from The Cancer Genome Atlas (TCGA) was downloaded as the training data, and transcriptome data of GSE15622, GSE102073, GSE19829, and GSE26712 were retrieved from Gene Expression Omnibus (GEO) as the validation cohorts. Differentially expressed genes (DEGs) were selected between platinum-sensitive and platinum-resistant patients from the training cohort, and multiple machine-learning algorithms [including random forest, XGboost, and least absolute shrinkage and selection operator (LASSO) regression] were utilized to determine the candidate genes from DEGs. Then, we applied logistic regression to establish the IPR signature based on the expression. Finally, comprehensive clinical, genomic, and survival feature were analyzed to understand the application value of the established IPR signature.

Results: A total of 532 DEGs were identified between platinum-resistant and platinum-sensitive samples, and 11 of them were shared by these three-machine learning algorithms and utilized to construct an IPR prediction signature. The area under receiver operating characteristic curve (AUC) was 0.841 and 0.796 in the training and validation cohorts, respectively. Notably, the prediction capacity of this signature was stable and robust regardless of the patients' homologous recombination deficiency (HRD) and mutation burden status. Meanwhile, the genomic feature was concordant between samples with high- or low-IPR signature, except a significantly higher prevalence of gain at Chr19q.12 (regions including ) in the high-IPR signature samples. The efficacy of prediction of platinum resistance of IPR signature successfully transferred to the precise survival prediction, with the AUC of 0.71, 0.72, and 0.66 to predict 1-, 3-, and 5-year survival, respectively. At last, we found a significantly different tumor-infiltrated lymphocytes feature, including lower abundance of CD4+ naive T cells in the samples with high-IPR signature. A relatively lower tumor immune dysfunction and exclusion (TIDE) value and more sensitivity to multiple therapies including Gefitinib may suggest the potency to transfer from platinum-based therapy to immunotherapy or target therapies in patients with high-IPR signature.

Conclusion: Our study established an IPR signature based on the expression of 11 genes that could stably and robustly distinguish OC patients with IPR and/or poor outcomes, which may guide therapeutic regimes tailoring.
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http://dx.doi.org/10.3389/fonc.2022.847085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971787PMC
March 2022

Application Effect of Doctor-Nurse-Patient Integration Model Based on Heart Rate Management Strategies in Middle-Aged and Young Outpatients with Hypertension.

Comput Math Methods Med 2022 16;2022:7459518. Epub 2022 Mar 16.

Outpatient Department, HeJi Hospital, Changzhi Medical College, Changzhi, 046000 Shanxi Province, China.

Objective: In this study, a doctor-nurse-patient integration management scheme based on heart rate (HR) management strategies was constructed to explore its application effect in the health management of young and middle-aged outpatients with hypertension.

Methods: A total of 75 young and middle-aged patients with essential hypertension admitted to the Outpatient Department of Cardiology of Heji Hospital Affiliated to Changzhi Medical College, Shanxi Province, from October 2019 to July 2020 were selected and divided into an observation group ( = 37) and a control group ( = 38) according to different treatment methods. The control group was treated with routine health education, basis on which, the observation group was additionally intervened by the doctor-nurse-patient integration model established upon HR management strategies. The resting heart rate (RHR) awareness, medication compliance, RHR, systolic/diastolic blood pressure (SBP/DBP), and self-management ability were compared between the two groups before and 3 months after intervention. The diet control rate, hypertension awareness rate, and regular review rate were also compared.

Results: The RHR awareness and medication compliance were significantly higher in the observation group compared with the control group after intervention ( < 0.05). There were 29 patients with high compliance in the observation group and 19 in the control group, with a significant difference between the two groups ( < 0.05). The mean RHR, as well as the mean SBP and DBP in the observation group, were significantly lower than those in the control group ( < 0.05). In terms of health behavior assessment, the observation group outperformed the control group in the score of each dimension of self-actualization, health responsibility, stress management, interpersonal support, exercise, and nutrition ( < 0.05). In addition, the self-management ability of diet, exercise, medication, blood pressure (BP) monitoring, and disease awareness was significantly higher in the observation group compared with the control group.

Conclusions: For middle-aged and young outpatients with hypertension, the doctor-nurse-patient integration model based on HR management strategies can improve the RHR awareness of patients and improve their medication compliance and self-management ability, thus better controlling the levels of RHR and BP.
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http://dx.doi.org/10.1155/2022/7459518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8942681PMC
April 2022

HSPA6 is Correlated With the Malignant Progression and Immune Microenvironment of Gliomas.

Front Cell Dev Biol 2022 23;10:833938. Epub 2022 Feb 23.

Departments of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

Gliomas are primary intracranial space lesions with a high mortality rate. Current treatments for glioma are very limited. Recently, immunotargeted therapy of the glioma microenvironment has been developed. Members of the 70 kDa heat shock protein (HSP70) family are involved in the development of many tumors and immunity. HSPA6 protein belongs to the HSP70 family; However, the biological function of this protein in gliomas has yet to be evaluated. In the present study, a range of analyses, involving protein networks, survival, clinical correlation, and function, revealed that the expression of HSPA6 was negatively correlated with clinical prognosis and closely associated with immunity, invasion, and angiogenesis. Quantitative protein analysis confirmed that HSPA6 was expressed at high levels in patients with glioblastoma. Vitro experiments further verified that HSPA6 enhanced the malignant progression of glioma cells by promoting proliferation, invasion and anti-apoptosis. We also found that HSPA6 was closely correlated with genomic variations and tumor microenvironment. Collectively, we demonstrated that HSPA6 may represent a new therapeutic target to improve the prognosis of patients with gliomas.
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http://dx.doi.org/10.3389/fcell.2022.833938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904718PMC
February 2022

Author Correction: Characteristics of SARS-CoV-2 and COVID-19.

Nat Rev Microbiol 2022 May;20(5):315

CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, People's Republic of China.

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http://dx.doi.org/10.1038/s41579-022-00711-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864972PMC
May 2022
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