Publications by authors named "Hua Cheng"

315 Publications

Spatial Coherence Manipulation on the Disorder-Engineered Statistical Photonic Platform.

Nano Lett 2022 Aug 25;22(15):6342-6349. Epub 2022 Jul 25.

The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Renewable Energy Conversion and Storage Center, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, China.

Coherence, similar to amplitude, polarization, and phase, is a fundamental characteristic of the light fields and is dominated by the statistical optical property. Although spatial coherence is one of the pivotal optical dimensions, it has not been significantly manipulated on the photonic platform. Here, we theoretically and experimentally manipulate the spatial coherence of light fields by loading different random phase distributions onto the wavefront with a metasurface. We achieve the generation of partially coherent light with a predefined degree of coherence and continuously modulate it from coherent to incoherent by controlling the phase fluctuation ranges or the beam sizes. This design strategy can be easily extended to manipulate arbitrary phase-only special beams with the same degree of coherence. Our approach provides straightforward rules to manipulate the coherence of light fields in an extra-cavity-based manner and paves the way for further applications in ghost imaging and information transmission in turbulent media.
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http://dx.doi.org/10.1021/acs.nanolett.2c02115DOI Listing
August 2022

and Synergistically Regulate the Transformation from Completely Mixed Flower Buds to Female Flower Buds in .

Int J Mol Sci 2022 Jun 9;23(12). Epub 2022 Jun 9.

School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China.

Chestnut () is an important woody food crop, but its yield has been low in cultivation, mainly due to the problems of fewer female flowers and more male flowers. Therefore, regulating the transition of chestnut flowers and effectively balancing the proportion of male and female to improve the yield are key factor to be solved in production. In this study, the chestnut floral buds in pre- and post-winter were used as materials. The data of metabolites, hormones, and gene expression during flower bud differentiation of chestnut were analyzed by transcriptomics and metabolomics to preliminarily reveal the possible reason of male and female flower bud transformation in pre- and post-winter. The analysis of Differentially Expressed Genes (DEGs) showed that there were 6323 DEGs in the Complete mixed flower bud (CMF) group in pre- and post-winter, of which 3448 genes were up-regulated and 2875 genes were down-regulated. There were 8037 DEGs in the Incomplete mixed flower bud (IMF) in pre- and post-winter, of which 4546 genes were up-regulated and 3491 genes were down-regulated. A total of 726 genes from the two flower buds were enriched into 251 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in post winter, of which plant hormone signal transduction accounted for 4.13%. The analysis results of differential metabolites showed that the differential metabolites of the two flower buds were mainly concentrated in the secondary metabolic synthesis pathway. The difference of hormone content showed that the content of Gibberellin 9 (GA9) and GA19 in CMF was higher than that in IMF in pre-winter, but the opposite in post-winter. Methyl jasmonate (MeJA) content was only very high in CMF in pre-winter, while Jasmonoyl-(l)-Isoleucine (JA-ILE) showed high content in CMF in post-winter. In post-winter, higher concentration of JA-ILE was positively correlated with the expression of Flowering Locus T (), and gene was significantly positively correlated with the expression levels of , and (LEAFY 3). The higher concentration of JA-ILE was negatively correlated with the transcription level of . In vitro experiments further verified that Jasmonate-Zim 1-3 (JAZ 1-3) combined with MYC2-1 inhibited the transcription of gene, while MYC2-1 alone promoted the expression of . The results suggested that a higher concentration of GA is conducive to breaking the dormancy of flower buds and promoting the development of male flower buds, while a lower concentration of GA and a higher concentration of JA-ILE are conducive to the differentiation and formation of female flower buds in post-winter, in which and play a key role in the differentiation of female flower buds of chestnut.
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http://dx.doi.org/10.3390/ijms23126452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224291PMC
June 2022

Copper isotope ratios allowed for quantifying the contribution of coal mining and combustion to total soil copper concentrations in China.

Environ Pollut 2022 Sep 12;308:119613. Epub 2022 Jun 12.

School of Resources and Environmental Engineering, Anhui University, Anhui Province Engineering Laboratory for Mine Ecological Remediation, Hefei, 230601, Anhui, China.

The most prominent source of Cu contamination in soils is metal mining and processing, partly since the Middle Age. However, coal mining and combustion can also cause (some) Cu contamination. We studied the distribution of Cu concentrations and isotope ratios in soils of the Huaibei coal mining area. The contribution of the coal mining and combustion to total Cu concentrations in soil was determined with a two-end-member mixing model based on the distinct δCu values of the Cu emitted from coal mining and combustion and in native soil. The mean Cu concentration of 75 mg kg exceeded the local soil background value (round to 22.13 mg kg). The similar δCu value of grass near the coal mining and combustion operation as in gangue and flying ash indicated a superficial Cu contamination. Mining input was the dominant source of Cu in the contaminated soils, contributing up to 95% and on average 72% of the total Cu in the topsoils. The mining-derived Cu was leached to a depth of 65 cm, where still 29% of the Cu could be attributed to the mining emissions. Grasses showed lower δCu values than the topsoils, because of the preferential uptake of light Cu isotopes. However, the ΔCu was lower in the contaminated than the uncontaminated area because of superficial adsorption of isotopically heavy Cu from the mining emissions. Overall, in this study the distinct δCu values of the mining-derived Cu emissions and the native soil allowed for the quantification of the mining-derived Cu and had already reached the subsoil and contaminated the grass by superficial adsorption in only 60 years of mining operation.
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http://dx.doi.org/10.1016/j.envpol.2022.119613DOI Listing
September 2022

Analysis of the Global Disease Burden of Down Syndrome Using YLDs, YLLs, and DALYs Based on the Global Burden of Disease 2019 Data.

Front Pediatr 2022 29;10:882722. Epub 2022 Apr 29.

Department of Pharmacy, Xi'an Children's Hospital, Xi'an, China.

Purpose: This study aimed to determine Down syndrome (DS) burden using years lived with disability (YLDs), years of life lost (YLLs), disability-adjusted life years (DALYs), and the trends in these parameters.

Methods: We obtained the annual YLDs, YLLs, DALYs, and age-standardized rates (ASRs) of DS from 2010 to 2019 using the Global Health Data Exchange tool. The estimated annual percentage changes (EAPCs) in ASR were used to quantify and evaluate DS burden trends. Gaussian-process regression and Pearson's correlation coefficient were used to assess the relationship between DS burden and socio-demographic index (SDI).

Results: Global DALYs decreased by 2.68% from 2010 to 2019 but the ASR was stable, which was mostly explained by the stability in the ASR for YLLs. The ASR of YLDs showed an increasing trend (EAPC = 1.07, 95% CI = 0.45 to 1.69). There was notable regional imbalance, with most of the DALYs or ASRs in areas with relatively low SDI. The DALY rates of DS were mostly from the YLLs of children younger than 1 year. Lower SDI areas tended to have higher DS burdens (ρ = -0.3, < 0.001).

Conclusion: This systematic analysis of the global disease burden of DS from 2010 to 2019 revealed that although the global DS DALY and YLL rate is stable, the YLD rate is increasing. And the DS burden varies significantly differences among regions or countries. The present results suggest that future strategies should focus on DS-related deaths in children younger than 1 year and the DS burden in low-SDI regions or countries, since this may be effective in further reducing DS burden.
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http://dx.doi.org/10.3389/fped.2022.882722DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099075PMC
April 2022

Significance of Nerve Plane for Inferior Mesenteric Plexus Preservation in Laparoscopic Rectal Cancer Surgery.

Front Oncol 2022 22;12:853662. Epub 2022 Apr 22.

Department of Gastrointestinal Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Station 253 node dissection with high ligation of the inferior mesenteric artery (IMA) is difficult to perform without damage to the surrounding autonomic nerve plexuses. This study aimed to investigate the significance of the nerve plane for inferior mesenteric plexus (IMP) preservation in laparoscopic rectal cancer surgery.

Methods: A total of 56 consecutive rectal patients underwent laparoscopic en bloc station 253 node dissection with high ligation of the IMA. Station 253 nodes were divided into the extra- and intra-nerve plane station 253 nodes for further H&E staining and immunohistochemical analysis. Based on IMP nerve plane-based evidence and histopathological results, a novel nerve-sparing technique, IMP nerve plane orientation, was proposed and performed on 68 rectal cancer patients. Urinary and sexual functions in all patients were evaluated at 6 months postoperatively.

Results: Lymph node metastasis was not found, but abundant nerve bundles containing gangliocytes were observed in extra-nerve plane station 253 nodes. The nerve plane was identified intraoperatively and then confirmed by both postoperative gross specimen evaluation and histopathological analysis. The novel nerve-sparing technique (IMP nerve plane orientation) was successfully performed with no postoperative complications, and the operated patients had improved postoperative urinary and sexual functions.

Conclusion: The nerve plane is helpful for IMP preservation and station 253 node dissection. This novel nerve-sparing technique of nerve plane orientation is technically feasible and safe, which could result in faster recovery of urinary and sexual functions.
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http://dx.doi.org/10.3389/fonc.2022.853662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072964PMC
April 2022

Protective effect of laparoscopic functional total mesorectal excision on urinary and sexual functions in male patients with mid-low rectal cancer.

Asian J Surg 2022 Mar 28. Epub 2022 Mar 28.

Department of Gastrointestinal Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, China. Electronic address:

Background: Urinary and sexual dysfunctions are among the most common complications in rectal cancer surgery. This study aimed to investigate the protective effect of laparoscopic functional total mesorectum excision (TME) on urinary and sexual functions in male patients.

Methods: A total of 248 male patients with mid-low rectal cancer were recruited in this study between February 2017 and July 2020. To overcome selection bias, we performed a 1:1 match using six variables, including age, BMI, ASA score, tumor distance, clinical T stage, and tumor size. The urinary function was assessed by the International Prostate Symptom Score (IPSS), sexual function was assessed by a 5-item version of the International Index of Erectile Function (IIEF-5) and ejaculation grading at postoperative 3 and 12 months.

Results: 79 patients received functional TME surgery (FTME group), and 169 patients received routine TME surgery (RTME group). After the propensity score, 79 pairs were balanced and analyzed. Patients in the FTME group showed a lower IPSS score and higher IIEF-5 score than patients in the RTME group at postoperative 3 and 12 months. The incidence of ejaculation dysfunction for patients in the FTME group was lower than patients in the RTME group at postoperative 3 and 12 months.

Conclusion: Laparoscopic functional total mesorectal excision was beneficial to faster recovery of urinary and sexual function for patients with rectal cancer, and it could be used as a superior surgical technique for pelvic autonomic nerve preservation in mid-low rectal cancer.
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http://dx.doi.org/10.1016/j.asjsur.2022.03.050DOI Listing
March 2022

Flattened Structural Network Changes and Association of Hyperconnectivity With Symptom Severity in 2-7-Year-Old Children With Autism.

Front Neurosci 2021 14;15:757838. Epub 2022 Feb 14.

Department of Radiology, Children's Hospital of Philadelphia, Philadelphia, PA, United States.

Understanding the brain differences present at the earliest possible diagnostic age for autism spectrum disorder (ASD) is crucial for delineating the underlying neuropathology of the disorder. However, knowledge of brain structural network changes in the early important developmental period between 2 and 7 years of age is limited in children with ASD. In this study, we aimed to fill the knowledge gap by characterizing age-related brain structural network changes in ASD from 2 to 7 years of age, and identify sensitive network-based imaging biomarkers that are significantly correlated with the symptom severity. Diffusion MRI was acquired in 30 children with ASD and 21 typically developmental (TD) children. With diffusion MRI and quantified clinical assessment, we conducted network-based analysis and correlation between graph-theory-based measurements and symptom severity. Significant age-by-group interaction was found in global network measures and nodal efficiencies during the developmental period of 2-7 years old. Compared with significant age-related growth of the structural network in TD, relatively flattened maturational trends were observed in ASD. Hyper-connectivity in the structural network with higher global efficiency, global network strength, and nodal efficiency were observed in children with ASD. Network edge strength in ASD also demonstrated hyper-connectivity in widespread anatomical connections, including those in default-mode, frontoparietal, and sensorimotor networks. Importantly, identified higher nodal efficiencies and higher network edge strengths were significantly correlated with symptom severity in ASD. Collectively, structural networks in ASD during this early developmental period of 2-7 years of age are characterized by hyper-connectivity and slower maturation, with aberrant hyper-connectivity significantly correlated with symptom severity. These aberrant network measures may serve as imaging biomarkers for ASD from 2 to 7 years of age.
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http://dx.doi.org/10.3389/fnins.2021.757838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882907PMC
February 2022

Random Network and Non-rich-club Organization Tendency in Children With Non-syndromic Cleft Lip and Palate After Articulation Rehabilitation: A Diffusion Study.

Front Neurol 2022 2;13:790607. Epub 2022 Feb 2.

Department of Radiology, National Center for Children's Health, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Objective: The neuroimaging pattern in brain networks after articulation rehabilitation can be detected using graph theory and multivariate pattern analysis (MVPA). In this study, we hypothesized that the characteristics of the topology pattern of brain structural network in articulation-rehabilitated children with non-syndromic cleft lip and palate (NSCLP) were similar to that in healthy comparisons.

Methods: A total of 28 children with NSCLP and 28 controls with typical development were scanned for diffusion tensor imaging on a 3T MRI scanner. Structural networks were constructed, and their topological properties were obtained. Besides, the Chinese language clear degree scale (CLCDS) scores were used for correlation analysis with topological features in patients with NSCLP.

Results: The NSCLP group showed a similar rich-club connection pattern, but decreased small-world index, normalized rich-club coefficient, and increased connectivity strength of connections compared to controls. The univariate and multivariate patterns of the structural network in articulation-rehabilitated children were primarily in the feeder and local connections, covering sensorimotor, visual, frontoparietal, default mode, salience, and language networks, and orbitofrontal cortex. In addition, the connections that were significantly correlated with the CLCDS scores, as well as the weighted regions for classification, were chiefly distributed in the dorsal and ventral stream associated with the language networks of the non-dominant hemisphere.

Conclusion: The average level rich-club connection pattern and the compensatory of the feeder and local connections mainly covering language networks may be related to the CLCDS in articulation-rehabilitated children with NSCLP. However, the patterns of small-world and rich-club structural organization in the articulation-rehabilitated children exhibited a random network and non-rich-club organization tendency. These findings enhanced the understanding of neuroimaging patterns in children with NSCLP after articulation rehabilitation.
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http://dx.doi.org/10.3389/fneur.2022.790607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847279PMC
February 2022

Dynamic Characteristics and Damage Constitutive Model of Mudstone under Impact Loading.

Materials (Basel) 2022 Jan 31;15(3). Epub 2022 Jan 31.

School of Civil Engineering and Architecture, Anhui University of Science and Technology, Huainan 232001, China.

The mechanical response characteristics of mudstone from the ingate roadway of the west ventilation shaft in Yuandian No. 2 coal mine, Huaibei City, Anhui Province, China to dynamic loads were quantified in single- and cyclic-impact compression tests, using the split-Hopkinson pressure bar test device. The dynamic stress-strain relationships and the failure characteristics of mudstone samples under different impact loads were analyzed systematically. Considering the "rate effect" of the mudstone dynamic strength, the dynamic strength criterion of mudstone was proposed, and the dynamic damage constitutive model of mudstone was established, based on the statistical damage theory. In response to single-impact loads, with increasing impact pressure, the mudstone peak stress and strain gradually increased, and the peak stress and average strain rate increased nonlinearly. In response to cyclic-impact loads, with an increasing number of impacts, the mudstone peak stress first increased and then decreased, and the peak strain increased gradually. With increasing impact pressure, the number of impacts to the samples' failure decreased gradually. By parameter identification and comparative analysis of the test results, the proposed dynamic damage constitutive model of mudstone was validated. The model can be used for stability analysis of roadway-surrounding rock under dynamic loads.
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http://dx.doi.org/10.3390/ma15031128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839729PMC
January 2022

Leigh Syndrome: A Study of 209 Patients at the Beijing Children's Hospital.

Ann Neurol 2022 04 6;91(4):466-482. Epub 2022 Mar 6.

Department of Neurology, National Center for Children's Health, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Objective: Leigh syndrome (LS) is a heterogeneous neurodegenerative disease and the most frequent pediatric manifestation of mitochondrial disease. In the largest patient collection to date, this study aimed to provide new insights into the clinical and genetic spectrum of LS, defect-specific associations, and predictors of disease course and survival.

Methods: Clinical, metabolic, neuroimaging, onset, and survival data were collected from the medical records of 209 patients referred to the Beijing Children's Hospital with symmetrical basal ganglia and/or brainstem neuroimaging changes indicative of LS by 30 centers from the Chinese network of mitochondrial disease (mitoC-NET) between January 2013 and July 2021 for exploratory analysis.

Results: Pathogenic variants were identified in 52 genes, most frequently MT-ATP6, SURF1, and PDHA1. Maternally inherited variants accounted for 42% (heteroplasmy level ≥90% in 64%). Phenotypes spanned 92 Human Phenotype Ontology terms. Elevated serum lactate (144/195), global developmental delay (142/209), and developmental regression (103/209) were most frequent. Discriminating neuroimaging and/or clinical features were identified for MT-ATP6 (m.9176T>C), MT-ND5, PDHA1, SUCLG1, and SURF1. Poorest survival was associated with MT-ND5, MT-ATP6 (m.8993T>C and m.9176T>C), SURF1, and ALDH5A1 (≤50% 3 year's survival), in contrast to milder defects with specific treatment (ECHS1 and SLC19A3, 100% 3 year's survival).

Interpretation: Our data define phenotype, onset, and survival of LS in a defect-specific manner, identifying features discriminating between genetic defects and predictive of disease outcome. These findings are essential to early diagnosis, in optimizing family counseling, and to the design and monitoring of future clinical trials, the next frontier of LS research. ANN NEUROL 2022;91:466-482.
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http://dx.doi.org/10.1002/ana.26313DOI Listing
April 2022

Cs CO -Promoted C-O Coupling Protocol Enables Solventless (Hetero)aryl Ether Synthesis under Air Atmosphere.

Chem Asian J 2022 Mar 28;17(6):e202101370. Epub 2022 Jan 28.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P. R. China.

In this work, a Cs CO -promoted synthetic approach was identified for (hetero)aryl ether synthesis via the C-O coupling of various (hetero)aryl chlorides and alcohols/phenol. To our delight, the reactions could be carried out under transition-metal-free and solvent-free conditions. Moreover, analytical-grade reagents and air atmosphere were readily tolerated. To showcase the practical usefulness of the present protocol, the assembly of a bioactive molecule was facilely realized and the gram-scale production of selected ether products was also efficiently accomplished. In addition, density functional theory (DFT) studies, along with a few mechanistic experiments, were conducted to elucidate a proposed reaction pathway and rationalize the pivotal role of Cs CO in promoting this process. Hopefully, this work could provide useful information for researchers who are engaging in C-O cross-coupling reactions.
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http://dx.doi.org/10.1002/asia.202101370DOI Listing
March 2022

Learning curve of uniportal video-assisted thoracoscopic lobectomy: an analysis of the proficiency of 538 cases from a single centre.

Interact Cardiovasc Thorac Surg 2022 05;34(5):799-807

Department of Thoracic Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, China.

Objectives: Uniportal video-assisted thoracoscopic surgery (UniVATS) is widely used as a minimally invasive thoracic operation. The goal of our study was to analyse the effect of long-term experience with the UniVATS lobectomy on the learning curve.

Methods: The learning curves were quantitatively evaluated by the unadjusted cumulative sum, and they were segmented using joinpoint linear regression analysis. The variables were compared between subgroups using trend analysis, and linear regression analysis was applied to correlate clinical characteristics at different stages of the learning curve with the duration of the operation.

Results: The learning curve for the UniVATS lobectomy can be divided into 3 phases of proficiency at ∼200-300 procedures, with a fourth phase as the number of procedures increases. The 1st-52nd, 52nd-156th, 156th-244th and 244th-538th procedures comprised the preliminary learning stage, preliminary proficiency stage, proficiency stage and advanced proficiency stage, respectively. Surgical outcomes and their variability between stages improved with increasing case numbers, with the most significant addition of an auxiliary operating port and conversions. In multivariable analysis, as stages progressed, influences other than surgical experience increased the operative time, with male and extensive pleural adhesions in the preliminary proficiency stage; male and incomplete pulmonary fissures in the proficiency stage; and male, extensive pleural adhesions and incomplete pulmonary fissures in the advanced proficiency stage.

Conclusions: As the number of procedures increases, there may be 4 different proficiency stages in the UniVATS lobectomy learning curve. The surgeon enters the fourth stage at approximately the 244th procedure. Moreover, at stage 4, the perioperative indicators tend to stabilize, and influences other than surgical experience become more significant.
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http://dx.doi.org/10.1093/icvts/ivab378DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070520PMC
May 2022

Study on Durability and Pore Characteristics of Concrete under Salt Freezing Environment.

Materials (Basel) 2021 Nov 26;14(23). Epub 2021 Nov 26.

School of Civil Engineering, Xijing University, Xi'an 710123, China.

The macroscopic mechanical properties and frost resistance durability of concrete are closely related to the changes in the internal pore structure. In this study, the two-dimensional and three-dimensional ICT (Industrial Computerized Tomography) pore characteristics of C30 concrete specimens before and after freezing and thawing in clean water, 5 wt.% NaCl, 5 wt.% CaCl, and 5 wt.% CHCOOK solution environments are obtained through concrete frost resistance durability test and ICT scanning technology. The effects of pore structure changes on concrete frost resistance, durability, and compressive strength mechanical properties after freezing and thawing cycles in different salt solution environments are analyzed. This paper provides new means and ideas for the study of concrete pores. The results show that with the increase in the freezing and thawing times, the concrete porosity, two-dimensional pore area, three-dimensional pore volume, and pore number generally increase in any solution environment, resulting in the loss of concrete compressive strength, mortar spalling, and the decrease in the relative dynamic elastic modulus. Among them, the CHCOOK solution has the least influence on the concrete pore changes; the NaCl solution has the greatest influence on the change in the concrete internal porosity. The damage of CaCl solution to concrete is second only to the NaCl solution, followed by clean water. The increase in the concrete internal porosity from high to low is NaCl, CaCl, clean water, and CHCOOK. The change in the pore volume of 0.1 to 1 mm after the freeze-thaw cycle is the main factor for reducing concrete strength. The test results have certain guiding value for the selection of deicing salt in engineering.
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http://dx.doi.org/10.3390/ma14237228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658295PMC
November 2021

Long noncoding RNA TUG1 upregulates VEGFA to enhance malignant behaviors in stomach adenocarcinoma by sponging miR-29c-3p.

J Clin Lab Anal 2021 Dec 11;35(12):e24106. Epub 2021 Nov 11.

Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

Background: Long noncoding RNA (lncRNA) TUG1 has been reported to display a pivotal role in the tumorigenesis and malignant progression of various types of cancers, including stomach adenocarcinoma (STAD). However, the contribution of aberrant expression of TUG1 and the mechanism by which it serves as a competing endogenous RNA (ceRNA) in STAD remains largely obscure.

Methods: The human STAD cell lines (MGC-803 and AGS), human normal gastric epithelial cell line (GES-1), human umbilical vein endothelial cells (HUVECs), and human embryonic kidney cells (HEK293T) were purchased and cultured to investigate the roles of TUG1 in STAD. Twenty BALB/c nude mice were purchased to establish a xenograft model to explore the roles of TUG1 in vivo.

Results: Bioinformatics analysis revealed that TUG1 was upregulated in STAD, of which expression was negatively and positively correlated with miR-29c-3p and VEGFA, respectively. Functional analyses indicated that TUG1 functioned as an oncogene to promote malignant behaviors (proliferation, migration, and angiogenesis) of STAD cells; whereas miR-29c-3p exerted the opposite role. Mechanistically, the interaction between miR-29c-3p with TUG1 and VEGFA was demonstrated. It was observed that miR-29c-3p could reverse the TUG1-induced promotion effect on cell proliferation, migration, and angiogenesis in STAD. Furthermore, TUG1 overexpression promoted STAD cell proliferation, metastasis, and angiogenesis, whereas VEGFA silence restored these effects, both in vitro and in vivo.

Conclusion: This finding confirmed that lncRNA TUG1 acts as a ceRNA for miR-29c-3p to promote tumor progression and angiogenesis by upregulating VEGFA, indicating TUG1 as a therapeutic target in STAD management.
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http://dx.doi.org/10.1002/jcla.24106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649340PMC
December 2021

Metal-Free Synthesis of Heteroaryl Amines or Their Hydrochlorides via an External-Base-Free and Solvent-Free C-N Coupling Protocol.

J Org Chem 2021 11 16;86(21):14627-14639. Epub 2021 Oct 16.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, PR China.

Herein, a metal-free and solvent-free protocol was developed for the C-N coupling of heteroaryl halides and amines, which afforded numerous heteroaryl amines or their hydrochlorides without any external base. Further investigations elucidated that the basicity of amines and specific interactions derived from the X-ray crystallography analysis of played pivotal roles in the reactions. Moreover, this protocol was scalable to gram scales and applicable to drug molecules, which demonstrated its practical value for further applications.
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http://dx.doi.org/10.1021/acs.joc.1c01467DOI Listing
November 2021

Effective Structure Control of Colloidal Molecules and the Morphology Evolution Mechanism Investigation.

Langmuir 2021 Oct 14;37(42):12429-12437. Epub 2021 Oct 14.

Key Laboratory of Photovoltaic and Energy Conservation Materials, Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, People's Republic of China.

Colloidal molecules (CMs), nonspherical clusters of a small number of particles, can be used as building blocks for self-assembly applications. Here, we propose a novel one pot method for CMs synthesis. First, poly(-isopropylacrylamide--acrylic acid) (P(NIPAM--AA)) microgels were prepared by soap-free emulsion polymerization as seed particles, then monomer styrene and cross-linking agent divinylbenzene (DVB) were added, which could be polymerized by the remaining free radicals on the seed surface in situ. P(NIPAM--AA)-PS colloidal molecules with a series of morphologies such as popcorn-like, CO-like, NH-like, CH-like and so on could be obtained. The effects of satellite colloid viscosity, interfacial tension, and polymer chain mobility on the number of satellite colloid have been investigated, and the formation mechanism of CMs is proposed based on morphology evolution investigation. Compared with the existing CM synthesis techniques, our method enables fabricating CMs from vinyl monomer in a facile and efficient way, and the scientific finding regarding the CMs formation will guide the CMs fabrication toward salable and reliable direction.
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http://dx.doi.org/10.1021/acs.langmuir.1c02089DOI Listing
October 2021

Tunable dual-band and high-quality-factor perfect absorption based on VO-assisted metasurfaces.

Opt Express 2021 Sep;29(20):31488-31498

Perfect absorbers with high quality factors (Q-factors) are of great practical significance for optical filtering and sensing. Moreover, tunable multiwavelength absorbers provide a multitude of possibilities for realizing multispectral light intensity manipulation and optical switches. In this study, we demonstrate the use of vanadium dioxide (VO)-assisted metasurfaces for tunable dual-band and high-quality-factor perfect absorption in the mid-infrared region. In addition, we discuss the potential applications of these metasurfaces in reflective intensity manipulation and optical switching. The Q-factors of the dual-band perfect absorption in the proposed metasurfaces are greater than 1000, which can be attributed to the low radiative loss induced by the guided-mode resonances and low intrinsic loss from the constituent materials. By utilizing the insulator-metal transition in VO, we further proved that a continuous tuning of the reflectance with a large modulation depth (31.8 dB) can be realized in the designed metasurface accompanied by a dual-channel switching effect. The proposed VO-assisted metasurfaces have potential applications in dynamic and multifunctional optical devices, such as tunable multiband filters, mid-infrared biochemical sensors, optical switches, and optical modulators.
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http://dx.doi.org/10.1364/OE.436046DOI Listing
September 2021

Early-Age Hydration Reaction and Strength Formation Mechanism of Solid Waste Silica Fume Modified Concrete.

Molecules 2021 Sep 17;26(18). Epub 2021 Sep 17.

Shaanxi Key Laboratory of Safety and Durability of Concrete Structures, Xijing University, Xi'an 710123, China.

Solid waste silica fume was used to replace fly ash by different ratios to study the early-age hydration reaction and strength formation mechanism of concrete. The change pattern of moisture content in different phases and micro morphological characteristics of concrete at early age were analyzed by low field nuclear magnetic resonance (LF-NMR) and scanning electron microscope (SEM). The results showed that the compressive strength of concrete was enhanced optimally when the replacement ratio of solid waste silica fume was 50%. The results of LF-NMR analysis showed that the water content of modified concrete increased with the increase of solid waste silica fume content. The compressive strength of concrete grew faster within the curing age of 7 d, which means the hydration process of concrete was also faster. The micro morphological characteristics obtained by SEM revealed that the concrete was densest internally when 50% fly ash was replaced by the solid waste silica fume, which was better than the other contents.
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http://dx.doi.org/10.3390/molecules26185663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467122PMC
September 2021

Optimization of Burgers creep damage model of frozen silty clay based on fuzzy random particle swarm algorithm.

Sci Rep 2021 Sep 23;11(1):18974. Epub 2021 Sep 23.

School of Civil Engineering, Nantong Vocational University, Nantong, 226001, China.

The creep characteristics of frozen rock and soil are crucial for construction safety in cases of underground freezing. Uniaxial compression tests and uniaxial creep tests were performed at temperatures of - 10, - 15, - 20, and - 25 °C for silty clay used in Nantong metro freezing construction to investigate the effect law of the stress-strain curves and creep curves. However, owing to the complex effects of factors such as temperature and ground pressure, the mechanical properties of underground frozen silty clay are uncertain. The Burgers creep damage model was established by using an elastic damage element to simulate the accelerated creep stage. The traditional particle swarm optimization algorithm was improved using the inertia weight and the fuzzy random coefficient. The creep parameters of the Burgers damage model were optimized using the improved fuzzy random particle swarm algorithm at different temperatures and pressure levels. Engineering examples indicated that the optimized creep model can more effectively characterize the creep stages of frozen silty clay in Nantong metro freezing construction. The improved fuzzy random particle swarm algorithm has wider engineering applicability and faster convergence than the traditional algorithm.
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http://dx.doi.org/10.1038/s41598-021-98374-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460797PMC
September 2021

Increased modularity of the resting-state network in children with nonsyndromic cleft lip and palate after speech rehabilitation.

Brain Behav 2021 08 3;11(8):e02094. Epub 2021 Aug 3.

Department of Radiology, Beijing Children's Hospital, National Center for Children's Health, Capital Medical University, Beijing, China.

Introduction: Speech therapy is the primary management followed the physical management through surgery for children with nonsyndromic cleft lip and palate (NSCLP). However, the topological pattern of the resting-state network after rehabilitation remains poorly understood. We aimed to explore the functional topological pattern of children with NSCLP after speech rehabilitation compared with healthy controls.

Methods: We examined 28 children with NSCLP after speech rehabilitation (age = 10.0 ± 2.3 years) and 28 healthy controls for resting-state functional MRI. We calculated functional connections and the degree strength, betweenness centrality, network clustering coefficient (Cp), characteristic path length (Lp), global network efficiency (Eg), local network efficiency (Eloc), modularity index (Q), module number, and participation coefficient for the between-group differences using two-sample t tests (corrected p < .05). Additionally, we performed a correlation analysis between the Chinese language clear degree scale (CLCDS) scores and topological properties in children with NSCLP.

Results: We detected significant between-group differences in the areas under the curve (AUCs) of degree strength and betweenness centrality in language-related brain regions. There were no significant between-group differences in module number, participation coefficient, Cp, Lp, Eg, or Eloc. However, the Q (density: 0.05-0.30) and Q (t = 2.46, p = .02) showed significant between-group differences. Additionally, there was no significant correlation between topological properties of statistical between-group differences and CLCDS scores.

Conclusions: Although nodal metric differences existed in the language-related brain regions, the children with NSCLP after speech rehabilitation had similar global network properties, module numbers, and participation coefficient, but increased modularity. Our results suggested that children with NSCLP achieved speech rehabilitation through function specialization in the language-related brain regions. The resting-state topology pattern could be of substantive neurobiological importance and potential imaging biomarkers for speech rehabilitation.
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http://dx.doi.org/10.1002/brb3.2094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413807PMC
August 2021

Multi-band on-chip photonic spin Hall effect and selective excitation of whispering gallery modes with metasurface-integrated microcavity.

Opt Lett 2021 Aug;46(15):3528-3531

We propose an approach to realize a multi-band on-chip photonic spin Hall effect and selective excitation of whispering gallery modes (WGMs) by integrating metasurfaces with microcavities. Free-space circularly polarized light with opposite spin angular momentum can effectively excite WGMs with opposite propagation directions at fixed wavelengths. Moreover, the different WGMs with different propagation directions and polarizations can be selectively excited by manipulating the number of antennas. We demonstrate that the optical properties (i.e., coupling efficiency, peak positions, and peak widths) of the proposed metasurface-integrated microcavities can be easily tailored by adjusting different geometric parameters. This study enables the realization of chiral microcavities with exciting novel functionalities, which may provide a further step in the development of photonic integrated circuits, optical sensing, and chiral optics.
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http://dx.doi.org/10.1364/OL.429940DOI Listing
August 2021

Myricitrin inhibits vascular endothelial growth factor-induced angiogenesis of human umbilical vein endothelial cells and mice.

Biomed Pharmacother 2020 Oct 17;130:110726. Epub 2020 Sep 17.

Department of Medical Cardiology, The General Hospital of NingXia Medical University, Yinchuan, 750004, China. Electronic address:

In the present study, the protective effects of myricitrin against vascular endothelial growth factor (VEGF)-induced angiogenesis of vascular endothelial cells were characterized. Cells were induced with 50 ng/mL VEGF in the presence or absence of various concentrations of myricitrin for 24 h. Myricitrin treatment significantly reduced cell proliferation by more than 50 %. Cells treated with myricitrin showed significantly increased caspase 3/7 activity and apoptosis in a dose-dependent manner. Treatment with 1, 10, or 100 μM myricitrin significantly reduced matrix metalloproteinase (MMP) activity by 23.3 %, 46.2 %, or 64.3 %, respectively. Myricitrin significantly reduced MMP1 and MMP2 mRNA expression. Similarly, treatment with 1, 10, or 100 μM myricitrin reduced MMP1 protein expression by 10.5 %, 31.6 %, or 52.6 %, respectively, and MMP2 protein expression by 10.9 %, 28.2 %, or 43.5 %, respectively. Cells treated with myricitrin showed significant inhibition of cell migration as well as capillary tube and sprouting formation. Myricitrin treatment significantly reduced the VEGF level. Immune-deficient nude mice bearing U251 xenograft tumors were used to investigate the antiangiogenic effects of myricitrin in vivo. The results demonstrated that myricitrin treatment in vivo significantly inhibited U251 cell xenograft tumor growth, as confirmed by the decreases in tumor volume and tumor weight. VEGF expression is a key proangiogenic factor. Myricitrin treatment significantly reduced mRNA and protein VEGF expression. Taken together, these results indicate that myricitrin is a potential inhibitor of VEGF-induced angiogenesis.
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http://dx.doi.org/10.1016/j.biopha.2020.110726DOI Listing
October 2020

Second-line regimen for CNS-involved pediatric Langerhans cell histiocytosis.

Pituitary 2022 Feb 24;25(1):108-115. Epub 2021 Jul 24.

Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, National Center for Children's Health, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.

Purpose: Langerhans cell histiocytosis (LCH) can affect any organ. Central nervous system (CNS) involvement is rare, and its management is poorly understood. This study aimed to analyze the clinical response and prognosis of pediatric LCH with central diabetes insipidus (CDI) treated with second-line therapy with cytarabine (Ara-c), cladribine (2-cdA), dexamethasone, and vindesine.

Methods: This retrospective case series study included pediatric LCH with CDI treated at Beijing Children's Hospital affiliated with Capital Medical University (11/2012-01/2018). After the first-line 2009-LCH regimen, patients with active disease/worse response, relapse, or no significant improvement in risk organs, pituitary, or lung were given the second-line therapy. Baseline characteristics, clinical response and adverse reactions were observed.

Results: Twenty-six children with CDI and disappearance of hyperintensity in the posterior pituitary were included. They received "Regimen A" Ara-c + dexamethasone + vindesine (n = 7) or "Regimen B" Ara-c + dexamethasone + vindesine + 2-cdA (n = 19) as second-line therapy. There were 14 patients with CDI but without pituitary stalk thickening (PST) and 12 with CDI and PST. In patients with CDI alone, 4/4 patients receiving Regimen A and 3/10 receiving Regimen B improved. All patients with CDI and PST showed improvement for PST. The reappearance of hyperintensity at the posterior pituitary was observed in 10 patients with CDI. All 26 children were alive after a median follow-up of 40.5 months. There were no chemotherapy-related deaths.

Conclusion: A combined therapy with Ara-c, 2-cdA, dexamethasone, and vindesine could partially alleviate pituitary disease conditions in pediatric LCH with CNS involvement, with good tolerance.
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http://dx.doi.org/10.1007/s11102-021-01176-xDOI Listing
February 2022

Switchable and Scalable Heteroarylation of Primary Amines with 2-Chlorobenzothiazoles under Transition-Metal-Free and Solvent-Free Conditions.

J Org Chem 2021 08 21;86(15):10288-10302. Epub 2021 Jul 21.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, P. R. China.

2-Aminobenzothiazoles comprise a valuable structural motif, which prevails in versatile natural products and biologically active compounds. Herein, a switchable and scalable C-N coupling protocol was developed for the synthesis of these compounds from 2-chlorobenzothiazoles and primary amines. Gratifyingly, this protocol was achieved under transition-metal-free and solvent-free conditions. Moreover, introducing an appropriate amount of NaH completely switched the selectivity from mono- toward di-heteroarylation, and further investigations provided a rationale for this new finding. Furthermore, gram-scale synthesis of representative products and was realized by applying operationally simple and glovebox-free procedures, which revealed the practical usefulness of this work. Finally, evaluation of the quantitative green metrics provided evidence that our protocol was superior over the literature ones in terms of green chemistry and sustainability.
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http://dx.doi.org/10.1021/acs.joc.1c01019DOI Listing
August 2021

Rapid capturing of oil-degrading bacteria by engineered attapulgite and their synergistic remediation for oil spill.

J Colloid Interface Sci 2021 Dec 28;604:272-280. Epub 2021 Jun 28.

Frontiers Science Center for Deep Ocean Multispheres and Earth System/Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, 266100, Qingdao, P.R. China. Electronic address:

Hypothesis: High-efficiency dispersion and enhanced biodegradation play important roles in the treatment of oily wastewater. Due to the flaws of chemical surfactants, it is necessary to study the alternative dispersants that are eco-friendly and sustainable. Therefore, applying natural attapulgite (ATP) to coat Brevibacillus parabrevis for dispersion and biodegradation was studied.

Experiments: To capture negatively charged bacteria in water, ATP was modified by positively charged Poly (allylamine hydrochloride) (PAH). The capturing capability of Poly (allylamine hydrochloride)-attapulgite (PAH-ATP) particles for bacterial cells, emulsification of PAH-ATP particles and bacteria on oil, toxicity of PAH-ATP to bacteria, biodegradation of oil, etc., were comprehensively investigated.

Findings: PAH-ATP modified bacteria show a highly effective emulsification for oil due to the synergism of PAH-ATP and bacteria. The emulsion stabilized by (PAH-ATP)@bacteria presents small and stable oil droplets in one month, which is benefit for the following biodegradation. Compared with bare bacteria and PAH-ATP, PAH-ATP can capture bacteria to the surface of the oil droplets which can greatly improve the degradation of oil pollution. Importantly, the presence of PAH-ATP does not inhibit the reproduction and activity of bacteria. Treatment of oily wastewater by combining natural nanoparticles and oil-degrading bacteria has the advantages of economy, environmental protection, and sustainability.
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http://dx.doi.org/10.1016/j.jcis.2021.06.137DOI Listing
December 2021

The application of SFDI and LSI system to evaluate the blood perfusion in skin flap mouse model.

Lasers Med Sci 2022 Mar 2;37(2):1069-1079. Epub 2021 Jul 2.

Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, 31116, Republic of Korea.

The aim of this study is to evaluate whether the blood perfusion to tissues for detecting ischemic necrosis can be quantitatively monitored by spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI) in a skin flap mouse model. Skin flaps were made on Institute of Cancer Research (ICR) mice. Using SFDI and LSI, the following parameters were estimated: oxyhemoglobin (HbO), deoxyhemoglobin (Hb), total hemoglobin (THb), tissue oxygen saturation (StO), and speckle flow index (SFI). Histologically, epithelium thickness, collagen deposition, and blood vessel count of skin flap tissues were analyzed. Then, the correlation of SFDI and histological results was assessed by application of Spearman rank correlation method. As the result, the number of blood vessels and the percentage of collagen areas showed significant difference between the necrotic tissue group and the non-necrotic one. Especially, the necrotic tissue had a complete epithelial loss and loses its normal structure. We identified that SFDI/LSI parameters were significantly different between non-necrotic and necrotic tissue groups. Especially, all SFDI and LSI parameters measured on the 1st day after surgery showed significant difference between necrotic tissue and non-necrotic tissue. In addition, the number of blood vessel and percentage of collagen area were positively correlated with HbO and StO among SFDI/LSI parameters. Meanwhile, the number of blood vessel and percentage of collagen area showed the negative correlation with Hb. By applying SFDI and LSI simultaneously to the skin flap, we could quantitatively monitor the blood perfusion and the tissue condition which can help us to detect ischemic necrosis objectively in early stage.
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http://dx.doi.org/10.1007/s10103-021-03354-6DOI Listing
March 2022

Modified strict sperm morphology threshold aids in the clinical selection of conventional fertilization (IVF) or intracytoplasmic sperm injection (ICSI).

Asian J Androl 2022 Jan-Feb;24(1):62-66

Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.

For infertility treatment, the selection of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) is decided by multiplying indicators (including fallopian tube factors, semen count, and semen motility), except for sperm morphology. In this study, we conducted a retrospective analysis, from implantation to birth, over a period of 5 years. A total of 1873 couples with primary or secondary fallopian tube factors and an increased defective sperm morphology rate (DSMR) were divided into different groups to receive IVF or ICSI cycles. By comparing the outcomes, we found that the F1 group (DSMR <96%, IVF group 1) had higher cleavage rate, biochemical pregnancy rate, clinical pregnancy rate, and live birth rate than the F3 group (DSMR >98%, IVF group 3; P < 0.05). In contrast, there was no significant difference in the ICSI subgroups. Furthermore, a comparison of the outcomes between IVF and ICSI showed that the S3 group (DSMR >98%, ICSI group 3) had higher cleavage rate (P < 0.001), biochemical pregnancy rate (P < 0.05), clinical pregnancy rate (P < 0.05) and live birth rate (P < 0.05) than the F3 group. However, the ICSI subgroup had a lower two pronuclei fertilization rate than the IVF subgroup (P < 0.05). Our data suggest that the sperm morphology should also be considered when selecting IVF or ICSI combined with other semen parameters before the first assisted reproductive technologies (ART) cycle, especially for males with severe sperm defects.
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http://dx.doi.org/10.4103/aja.aja_45_21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788610PMC
January 2022

Immunoglobulin (Ig) G4-related sclerosing cholangitis in patients resected for presumed perihilar cholangiocarcinoma: a 10-year experience.

Ann Transl Med 2021 Mar;9(5):415

Department of Biliary Surgery, West China Hospital, Sichuan University, Chengdu, China.

Background: This study aimed to investigate the incidence of immunoglobulin (Ig) G4-related sclerosing cholangitis (IgG4-SC) in patients resected for perihilar cholangiocarcinoma (PHC) in a designated hospital from 2010 to 2019. We also aimed to evaluate the diagnostic dilemma of IgG4-SC clinically.

Methods: Between January 2010 and December 2019, all patients who underwent radical resection due to presumed PHC were included. Independent pathologists scored bile duct samples based on the International Consensus Pathology Criteria for IgG4-related Disease (ICPD).

Results: Of the 289 patients who underwent radical resection of primary liver cancer, 26 (9%) were diagnosed as benign, without histological evidence of malignancy, among them, 23 had sclerosing inflammation, 1 had cystadenoma, and 2 had xanthogranulomatous cholangitis. Additionally, 18 had a definitive diagnosis of IgG4-SC. The misdiagnosis rate was 19% (54/289), of which, 26 patients had benign disease, and 28 patients had malignancies.

Conclusions: It is difficult to distinguish IgG-SC from PHC. The misdiagnosis has resulted in a large number of ineffective hepatectomies. Improving the detection rate of serum IgG4 (sIgG4) may therefore avoid misdiagnosis, surgery, and life-threatening complications.
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http://dx.doi.org/10.21037/atm-21-140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033389PMC
March 2021

Overexpression of an Phosphopyruvate Carboxylase Improves Plant Growth and Stress Tolerance.

Cells 2021 03 6;10(3). Epub 2021 Mar 6.

Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.

It has been challenging to simultaneously improve photosynthesis and stress tolerance in plants. Crassulacean acid metabolism (CAM) is a CO-concentrating mechanism that facilitates plant adaptation to water-limited environments. We hypothesized that the ectopic expression of a CAM-specific phosphopyruvate carboxylase (PEPC), an enzyme that catalyzes primary CO fixation in CAM plants, would enhance both photosynthesis and abiotic stress tolerance. To test this hypothesis, we engineered a CAM-specific gene (named ) from into tobacco. In comparison with wild-type and empty vector controls, transgenic tobacco plants constitutively expressing showed a higher photosynthetic rate and biomass production under normal conditions, along with significant carbon metabolism changes in malate accumulation, the carbon isotope ratio δC, and the expression of multiple orthologs of CAM-related genes. Furthermore, overexpression enhanced proline biosynthesis, and improved salt and drought tolerance in the transgenic plants. Under salt and drought stress conditions, the dry weight of transgenic tobacco plants overexpressing was increased by up to 81.8% and 37.2%, respectively, in comparison with wild-type plants. Our findings open a new door to the simultaneous improvement of photosynthesis and stress tolerance in plants.
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http://dx.doi.org/10.3390/cells10030582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999111PMC
March 2021

CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum.

Hortic Res 2021 Apr 1;8(1):79. Epub 2021 Apr 1.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

The CmBBX8-CmFTL1 regulatory module is a key determinant in the transition from vegetative growth to reproductive development in summer-flowering chrysanthemum. However, the detailed regulatory mechanism of CmBBX8-mediated flowering remains elusive. In this study, we revealed that RADICAL-INDUCED CELL DEATH 1 (CmRCD1) physically associated with CmBBX8 through bimolecular fluorescence complementation (BiFC), pulldown and Coimmunoprecipitation (CoIP) assays. Furthermore, the RCD1-SRO1-TAF4 (RST) domain of CmRCD1 and the B-box of CmBBX8 mediated their interaction. In addition, Luciferase (LUC) assays and electrophoretic mobility shift assay (EMSAs) showed that CmRCD1 repressed the transcriptional activity of CmBBX8 and interfered with its binding to the CmFTL1 promoter, thereby leading to delayed flowering in the summer chrysanthemum 'Yuuka'. These results provide insight into the molecular framework of CmRCD1-CmBBX8-mediated flowering in chrysanthemum.
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http://dx.doi.org/10.1038/s41438-021-00516-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012346PMC
April 2021
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