Publications by authors named "Hu Tao"

749 Publications

Association between glycated hemoglobin and ambulatory blood pressure or heart rate in hypertensive patients.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 May;46(5):488-496

Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

Objectives: To determine the association between glycosylated hemoglobin (HbA1c) and ambulatory blood pressure or heart rate in hypertensive patients.

Methods: A total of 585 patients, who performed ambulatory blood pressure monitoring (ABPM) from September 2018 to April 2019 in Xiangya Hospital, Central South University, were enrolled and assigned into 2 groups (470 in a hypertensive group and 115 in a normal group). HbA1c levels were compared. According to the HbA1c level, the hypertensive group was divided into 2 subgroups: A high HbA1c group (HbA1c≥6.1%, =142) and a normal HbA1c group (HbA1c<6.1%, =328). Whole basic data and ABPM parameter were compared among the groups. Pearson/Spearman correlation analysis was applied to study the association between HbA1c and BPV. Multivariate logistic regression was used to explore the influential factors for HbA1c (≥6.1%) and continuous increase of HbA1c in different hypertensive populations, respectively.

Results: The hypertensive group had higher HbA1c level than the normal group [(6.1±1.3)% vs (5.1±1.7)%, <0.05]. In hypertensive patients, nocturnal systolic blood pressure [(131.1±19.2) mmHg vs (122.5±19.2) mmHg], nocturnal systolic blood pressure load [62.5% (15.5%-100%) vs 28.6% (0-75%)], and daytime heart rate [(74.3±11.6) min vs (71.2±11.4) min] of the high HbA1c group were higher than those in the normal HbA1c group (all <0.05). Pearson/Spearman correlation analysis showed that HbA1c was positively correlated with systolic blood pressure and blood pressure load (both <0.05). Logistic regression analysis showed that nocturnal systolic pressure load was the risk factor for the increase of HbA1c level (OR=1.025, 95% CI 1.003 to 1.048, <0.05). Multiple linear regression showed that nocturnal systolic pressure load was still positively correlated with HbA1c in total, tertiary, and hypertensive patients without treatment (β=0.155, β=0.171, β=0.384, respectively, all <0.05).

Conclusions: In hypertensive patients, HbA1c is positively correlated with ambulate blood pressure, blood pressure load, and heart rate, and it has no correlation with blood pressure variability, heart rate variability, or morning blood pressure.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200750DOI Listing
May 2021

Identification of novel bat coronaviruses sheds light on the evolutionary origins of SARS-CoV-2 and related viruses.

Cell 2021 Jun 9. Epub 2021 Jun 9.

Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian 271000, China; School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian 271000, China. Electronic address:

Despite the discovery of animal coronaviruses related to SARS-CoV-2, the evolutionary origins of this virus are elusive. We describe a meta-transcriptomic study of 411 bat samples collected from a small geographical region in Yunnan province, China, between May 2019 and November 2020. We identified 24 full-length coronavirus genomes, including four novel SARS-CoV-2-related and three SARS-CoV-related viruses. Rhinolophus pusillus virus RpYN06 was the closest relative of SARS-CoV-2 in most of the genome, although it possessed a more divergent spike gene. The other three SARS-CoV-2-related coronaviruses carried a genetically distinct spike gene that could weakly bind to the hACE2 receptor in vitro. Ecological modeling predicted the co-existence of up to 23 Rhinolophus bat species, with the largest contiguous hotspots extending from South Laos and Vietnam to southern China. Our study highlights the remarkable diversity of bat coronaviruses at the local scale, including close relatives of both SARS-CoV-2 and SARS-CoV.
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http://dx.doi.org/10.1016/j.cell.2021.06.008DOI Listing
June 2021

Purification and characterization of the receptor-binding domain of SARS-CoV-2 spike protein from .

Eng Life Sci 2021 Jun 7;21(6):453-460. Epub 2021 May 7.

State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. China.

SARS-CoV-2 is responsible for a disruptive worldwide viral pandemic, and renders a severe respiratory disease known as COVID-19. Spike protein of SARS-CoV-2 mediates viral entry into host cells by binding ACE2 through the receptor-binding domain (RBD). RBD is an important target for development of virus inhibitors, neutralizing antibodies, and vaccines. RBD expressed in mammalian cells suffers from low expression yield and high cost. is a popular host for protein expression, which has the advantage of easy scalability with low cost. However, RBD expressed by (RBD-1) lacks the glycosylation, and its antigenic epitopes may not be sufficiently exposed. In the present study, RBD-1 was expressed by and purified by a Ni Sepharose Fast Flow column. RBD-1 was structurally characterized and compared with RBD expressed by the HEK293 cells (RBD-2). The secondary structure and tertiary structure of RBD-1 were largely maintained without glycosylation. In particular, the major β-sheet content of RBD-1 was almost unaltered. RBD-1 could strongly bind ACE2 with a dissociation constant (K) of 2.98 × 10 M. Thus, RBD-1 was expected to apply in the vaccine development, screening drugs and virus test kit.
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http://dx.doi.org/10.1002/elsc.202000106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182281PMC
June 2021

Single-Atom Cobalt-Based Electrochemical Biomimetic Uric Acid Sensor with Wide Linear Range and Ultralow Detection Limit.

Nanomicro Lett 2020 Oct 27;13(1). Epub 2020 Oct 27.

Institute of Materials Science and Devices, Suzhou University of Science and Technology, Suzhou, 215009, People's Republic of China.

Uric acid (UA) detection is essential in diagnosis of arthritis, preeclampsia, renal disorder, and cardiovascular diseases, but it is very challenging to realize the required wide detection range and low detection limit. We present here a single-atom catalyst consisting of Co atoms coordinated by an average of 3.4 N atoms on an N-doped graphene matrix (A-Co-NG) to build an electrochemical biomimetic sensor for UA detection. The A-Co-NG sensor achieves a wide detection range over 0.4-41,950 μM and an extremely low detection limit of 33.3 ± 0.024 nM, which are much better than previously reported sensors based on various nanostructured materials. Besides, the A-Co-NG sensor also demonstrates its accurate serum diagnosis for UA for its practical application. Combination of experimental and theoretical calculation discovers that the catalytic process of the A-Co-NG toward UA starts from the oxidation of Co species to form a Co-OH-UA*, followed by the generation of Co-OH + UA_H, eventually leading to N-H bond dissociation for the formation of oxidized UA molecule and reduction of oxidized Co to Co for the regenerated A-Co-NG. This work provides a promising material to realize UA detection with wide detection range and low detection limit to meet the practical diagnosis requirements, and the proposed sensing mechanism sheds light on fundamental insights for guiding exploration of other biosensing processes.
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http://dx.doi.org/10.1007/s40820-020-00536-9DOI Listing
October 2020

Mn as the "spearhead" preventing the trap of Zn in layered Mn inserted hydrated vanadium pentoxide enables high rate capacity.

J Colloid Interface Sci 2021 May 30;602:14-22. Epub 2021 May 30.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address:

Vanadium oxides attract much attention and are concerned as one of the most promising cathodes for aqueous zinc-ion batteries (AZIBs) owing to the layered structures. However, their intensive development is limited by the fragile structures and laggard ion-transferring. Herein, Mn inserted hydrated vanadium pentoxide nanobelts/reduced graphene oxide (MnVO·nHO/rGO, abbreviated as MnVOH/rGO) was prepared by a simple one-pot hydrothermal process, delivering excellent electrochemical properties for AZIBs. The Zn//MnVOH/rGO cell operates well even at changing current densities over 45 cycles, behaving 361 mAh·g at 0.1 A·g, 323 mAh·g as the current density gradually increasing to 2 A·g and 350 mAh·g when gradually back to 0.1 A·g (∼97% of initial capacity). Such a superb cycling and rate performance is ascribed to the unique stable structure with the compact electrostatic attraction between Mn and VO·nHO (VOH) laminate. On the one hand, Mn generates electrostatic network with [VO] polyhedrons and suppresses the following electrostatic trap for the moving Zn. On the other hand, rGO improves the conductivity, endowing the high capacity and energy density. The performance of the MnVOH/rGO cathode exceeds most of vanadium-based cathodes applying in AZIBs and paves the way to the ideal energy storage system.
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http://dx.doi.org/10.1016/j.jcis.2021.05.163DOI Listing
May 2021

Conjugation of haloalkane dehalogenase DhaA with arabinogalactan to increase its stability.

J Biotechnol 2021 Jul 9;335:47-54. Epub 2021 Jun 9.

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China. Electronic address:

Haloalkane dehalogenase DhaA can catalyze the hydrolytic cleavage of carbonhalogen bonds, along with production of the corresponding alcohol, a proton and a halide. However, DhaA suffers from poor environmental tolerance, such as sensitivity to high temperature, low pH and hypersaline. Arabinogalactan (AG) is a hydrophilic polysaccharide with highly branched long chains. DhaA was conjugated with AG to improve the environmental stability of DhaA in the present study. Each DhaA was averagely conjugated with 4∼5 AG molecules. Conjugation of AG essentially maintained the enzymatic activity of DhaA (91.4 %) without apparent structural alteration. The hydration layer formed by AG could reduce the solvent accessible area of DhaA and slow the protonation process, thereby improving the pH and high salt stability of DhaA. In particular, the remaining activities of the conjugate (AG-DhaA) were 35.3 % after treatment at pH4.0 for 1 h, and 80.8 % in 1 M NaCl after treatment for 16 h. As compared with DhaA, AG-DhaA showed slightly different kinetic parameters (K M of 1.90 μmol/L and k cat of 2.60 s -1).
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http://dx.doi.org/10.1016/j.jbiotec.2021.06.002DOI Listing
July 2021

A Novel Virus of Associated with Sexual Precocity in .

mSystems 2021 Jun 8:e0000321. Epub 2021 Jun 8.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Qingdao, China.

Since 2010, sexual precocity, a typical sign of the iron prawn syndrome (IPS), resulting in the reduced size of farmed giant freshwater prawns , has caused substantial production losses. However, the cause of IPS was not clear. We ran tests for eight major shrimp pathogens, but none were detected from IPS-affected prawns. We performed the histopathological examination of tissues and identified an eosinophilic inclusion in the perinuclear cytoplasm of cells in various tissues associated with nervous and endocrinal functions in the compound eyes. A subsequent bioassay with viral extracts of IPS-affected samples reproduced the gross signs of IPS. Metatranscriptomic sequencing identified a novel virus of in all IPS-affected prawns, which was not found in samples without IPS. This virus contains a positive-sense, single-stranded RNA genome of 12,630 nucleotides (nt). Phylogenetic analysis of the conserved RdRp and NS3 domains showed that it may belong to a new genus between Jingmenvirus and . Under transmission electron microscopy (TEM), putative virus particles showed as spherical with a diameter of 40 to 60 nm. hybridization found hybridization signals consistent with the histopathology in the compound eyes from IPS-affected . We provisionally name this virus infectious precocity virus (IPV) and propose the binominal Latin name gen. nov., sp. nov. We developed a nested reverse transcription-PCR diagnostic assay and confirmed that all IPS-affected prawns tested IPV positive but normal prawns tested negative. Collectively, our study revealed a novel virus of associated with sexual precocity in . The iron prawn syndrome (IPS), also described as sexual precocity, results in the reduced size of farmed prawns at harvest and significant economic losses. IPS has been frequently reported in populations of farmed since 2010, but the cause was heretofore unknown. Here, we reported a novel virus identified from prawns with IPS using infection experiments, metatranscriptomic sequencing, and transmission electron microscopy and provisionally named it infectious precocity virus (IPV). Phylogenetic analysis showed that IPV represents a new genus, proposed as gen. nov., in the family . This study provides novel insight that a viral infection may cause pathological change and sexual maturation and subsequently affect crustacean growth. Therefore, we call for quarantine inspection of IPV in transboundary trade of live and enhanced surveillance of IPV in aquaculture in the region and globally.
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http://dx.doi.org/10.1128/mSystems.00003-21DOI Listing
June 2021

Efficient synthesis of cyclic amidine-based fluorophores 6π-electrocyclic ring closure.

Chem Sci 2020 Mar 13;11(14):3586-3591. Epub 2020 Mar 13.

Guangdong Key Laboratory of Chiral Molecular and Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-Sen University Guangzhou 510006 China

Novel 10π-electron cyclic amidines with excellent fluorescence properties were synthesized by a general and efficient 6π-electrocyclic ring closure of ketenimine and imine starting from -sulfonyl triazoles and arylamines. The photophysical properties of cyclic amidine fluorophores have been studied in detail and have shown good properties of a large Stokes shift, pH insensitivity, low cytotoxicity and higher photostability, which have great potential for biological imaging. Furthermore, this novel fluorophore was successfully applied to the localization of the NK-1 receptor in living systems.
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http://dx.doi.org/10.1039/d0sc00798fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152618PMC
March 2020

Amelioration of Beta Interferon Inhibition by NS4B Contributes to Attenuating Tembusu Virus Virulence in Ducks.

Front Immunol 2021 17;12:671471. Epub 2021 May 17.

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, China.

Our previous studies reported that duck Tembusu virus nonstructural protein 2A (NS2A) is a major inhibitor of the IFNβ signaling pathway through competitively binding to STING with TBK1, leading to a reduction in TBK1 phosphorylation. Duck TMUV NS2B3 could cleave and bind STING to subvert the IFNβ signaling pathway. Here, we found that overexpression of duck TMUV NS4B could compete with TBK1 in binding to STING, reducing TBK1 phosphorylation and inhibiting the IFNβ signaling pathway by using the Dual-Glo Luciferase Assay System and the NanoBiT protein-protein interaction (PPI) assay. We further identified the E2, M3, G4, W5, K10 and D34 residues in NS4B that were important for its interaction with STING and its inhibition of IFNβ induction, which were subsequently introduced into a duck TMUV replicon and an infectious cDNA clone. We found that the NS4B M3A mutant enhanced RNA replication and exhibited significantly higher titer levels than WT at 48-72 hpi but significantly decreased mortality (80%) in duck embryos compared to WT (100%); the NS4B G4A and R36A mutants slightly reduced RNA replication but exhibited the same titer levels as WT. However, the NS4B R36A mutant did not attenuate the virulence in duck embryos, whereas the G4A mutant significantly decreased the mortality (70%) of duck embryos. In addition, the NS4B W5A mutant did not affect viral replication, whereas the D34A mutant slightly reduced RNA replication, and both mutants exhibited significantly lower titer levels than the WT and significantly decreased mortality (90% and 70%, respectively) in duck embryos. Hence, our findings provide new insight into the development of attenuated flaviviruses by targeting the disabling viral strategies used to evade the innate defense mechanisms.
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http://dx.doi.org/10.3389/fimmu.2021.671471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165282PMC
May 2021

Derivation of a Risk Score for High Caries Risk in 3- to 5-year-old Children in Sichuan Province.

Oral Health Prev Dent 2021 Jan;19(1):279-285

Purpose: To explore potential caries risk indicators in 3- to 5-year-old children, and develop a simple risk-score model to screen the children at high risk of caries with decayed, filled, and missing teeth (dmft) > 2.

Materials And Methods: A cross-sectional study involving 2746 children 3 to 5 years of age was conducted in Sichuan province. Children were examined for dmft index, and sociodemographic and behavioural factors were acquried through a questionnaire completed by their caregivers. A prediction model was developed by backward multivariate logistic regression, and its overfitting degree was examined with 5-fold cross-validation. A simple risk-score model was derived to screen the children with dmft > 2 at high risk of caries with the β regression coefficient obtained from the multivariate regression model.

Results: A child's oral health status was identified as the highest risk indicator with a β regression coefficient of 1.093. The mean area under curve (AUC) from the 5-fold cross-validation was 0.7408 (95% CI: 72.21%, 75.95%), with a bias of only ca 1%. This result allowed us to eliminate substantial overfitting of the prediction model. The AUC of the risk scoring system was 0.7455 (95% CI: 72.70%, 76.40%), which indicated good screenability.

Conclusions: This risk score model has the advantages of simplicity, low cost and relatively high accuracy, and is suitable for use in developing countries, especially for primary screening for high risk of caries. It shows that certain child behaviours and parental attitude play an important role in dental caries among preschool children.
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http://dx.doi.org/10.3290/j.ohpd.b1452865DOI Listing
January 2021

δ-Tocotrienol induces apoptosis and inhibits proliferation of nasopharyngeal carcinoma cells.

Food Funct 2021 May 31. Epub 2021 May 31.

Laboratory of Molecular Nutrition, College of Food Science and Engineering, National Engineering Laboratory for Deep Processing of Rice and Byproducts, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.

Nasopharyngeal carcinoma has a notably high incidence rate in Southern China, Southeast Asia, North Africa, Middle East, and the Arctic. δ-Tocotrienol is abundant in cereal and has some health benefits. In our recent study, we showed that δ-tocotrienol exerted anti-inflammatory effects in murine macrophages in vitro. The aim of this study was to further investigate the chemopreventive effects of δ-tocotrienol on human CNE1 cells. We showed that δ-tocotrienol induced apoptosis and cell cycle arrest at G0/G1 and M phases in nasopharyngeal carcinoma cells. Microarray analysis revealed that after CNE1 cells were treated with δ-tocotrienol, 169 genes were up-regulated and 167 down-regulated. ERK1/2 was shown to play a vital role in cell cycle arrest by gene chips. The results suggest that δ-tocotrienol induces cell cycle arrest in CNE1 cells via the p16/CDK4/cyclin D1 signaling pathway. Western blots showed that CNE1 apoptosis was related to dysregulated expression of Bax-2 and Bcl-2. Furthermore, caspase-3, -8, -9 up-regulation was related to the apoptotic effect of δ-tocotrienol; therefore, δ-tocotrienol triggers apoptosis in CNE1 cells through caspase-3 signaling. δ-Tocotrienol may potentially be developed as an anti-cancer agent in the management of nasopharyngeal carcinoma.
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http://dx.doi.org/10.1039/d1fo00461aDOI Listing
May 2021

TWIST2 inhibits EMT and induces oxidative stress in lung cancer cells by regulating the FGF21-mediated AMPK/mTOR pathway.

Exp Cell Res 2021 Aug 25;405(1):112661. Epub 2021 May 25.

Department of Thoracic Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, 264000, Shandong, People's Republic of China. Electronic address:

Twist related protein 2 (TWIST2) plays an important role in bone development, tumorigenesis, tumour progression and epithelial mesenchymal transition (EMT). At present, there are few reports about the role of TWIST2 in lung cancer, which need to be further explored. Therefore, the purpose of this study is to explore the role and molecular mechanism of TWIST2 in the occurrence and development of lung cancer. The expression of TWIST2 in tissues of patients and cell lines was measured using RT-qPCR and western blotting. MTT and CCK8 assays were used to detect cell proliferation and viability. Western blotting was used to measure the expression of EMT-related proteins, including E-cadherin, N-cadherin, Vimentin and Slug. The results revealed that TWIST2 is lowly expressed in the tissues of lung cancer patients and cell lines. Further studies found that overexpression of TWIST2 significantly induced apoptosis and promoted the expression of E-cadherin, as well as inhibiting the expression of N-cadherin, Vimentin and Slug. More importantly, TWIST2 induced oxidative stress in lung cancer cells. In addition, TWIST2 regulated the FGF21 and AMPK/mTOR signalling pathway, which is involved in the molecular mechanism of the gene in lung cancer cells. We suggest that the mechanism of TWIST2 inhibition of the progression of lung cancer is by regulating the FGF21-mediated AMPK/mTOR signalling pathway.
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http://dx.doi.org/10.1016/j.yexcr.2021.112661DOI Listing
August 2021

Learning spatiotemporal features of DSA using 3D CNN and BiConvGRU for moyamoya disease detection.

Int J Neurosci 2021 May 27:1-14. Epub 2021 May 27.

Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.

Moyamoya disease (MMD) is a serious intracranial cerebrovascular disease. Cerebral hemorrhage caused by MMD will bring life risk to patients. Therefore, MMD detection is of great significance in the prevention of cerebral hemorrhage. In order to improve the accuracy of digital subtraction angiography (DSA) in the diagnosis of MMD, in this paper, a deep network architecture combined with 3 D convolutional neural network (3 D CNN) and bidirectional convolutional gated recurrent unit (BiConvGRU) is proposed to learn the spatiotemporal features for MMD detection. Firstly, 2 D convolutional neural network (2 D CNN) is utilized to extract spatial features for each frame of DSA. Secondly, the long-term spatiotemporal features of DSA sequence are extracted by BiConvGRU. Thirdly, the short-term spatiotemporal features of DSA are further extracted by 3 D convolutional neural network (3 D CNN). In addition, different features are extracted when gray images and optical flow images pass through the network, and multiple features are extracted by features fusion. Finally, the fused features are utilized to classify. The proposed method was quantitatively evaluated on a data sets of 630 cases. The experimental results showed a detection accuracy of 0.9788, sensitivity and specificity were 0.9780 and 0.9796, respectively, and area under curve (AUC) was 0.9856. Compared with other methods, we can get the highest accuracy and AUC. The experimental results show that the proposed method is stable and reliable for MMD detection, which provides an option for doctors to accurately diagnose MMD.
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http://dx.doi.org/10.1080/00207454.2021.1929214DOI Listing
May 2021

Discovery of indoline derivatives as anticancer agents via inhibition of tubulin polymerization.

Bioorg Med Chem Lett 2021 Aug 20;45:128131. Epub 2021 May 20.

School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. Electronic address:

Human esophageal squamous cell carcinoma (ESCC) is one of the most lethal cancers in human digestive system. It is necessary to discover novel antitumor agents for the treatment of esophageal cancers because of its poor prognosis. Indoline has been reported as an efficient anticancer fragment to design novel anticancer agents. In this work, indoline derivatives were designed, synthesized and explored their anticancer activity. Compound 9d, which exhibited potent antiproliferative activity with IC values of 1.84 μM (MGC-803 cells), 6.82 μM (A549 cells), 1.61 μM (Kyse30 cells), 1.49 μM (Kyse450 cells), 2.08 μM (Kyse510 cells) and 2.24 μM (EC-109 cells), respectively. The most active compound 9d was identified as a tubulin inhibitor targeting colchicine binding site with an IC value of 3.4 µM. Compound 9d could strongly suppress the tubulin polymerization in Kyse450 cells. The results of molecular docking also suggested compound 9d could tightly bind into the colchicine binding site of β-tubulin. Besides, compound 9d inhibited the growth of KYSE450 cells in time and dose-dependent manners. All the results suggest that the indoline derivatives might be a class of novel tubulin inhibitors with potential anticancer activity and is worthy of further study.
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http://dx.doi.org/10.1016/j.bmcl.2021.128131DOI Listing
August 2021

Overexpression of FaHSP17.8-CII improves cadmium accumulation and tolerance in tall fescue shoots by promoting chloroplast stability and photosynthetic electron transfer of PSII.

J Hazard Mater 2021 May 5;417:125932. Epub 2021 May 5.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430074, China; Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan 430074, China. Electronic address:

Genetic improvement could play a significant role in enhancing the Cd accumulation, translocation and tolerance in plants. In this study, for the first time, we constructed transgenic tall fescue overexpressing a class II (CII) sHSP gene FaHSP17.8-CII, which enhanced Cd tolerance and the root-to-shoot Cd translocation. After exposed to 400 μM CdCl, two FaHSP17.8-CII overexpressing lines (OE#3 and OE#7) exhibited 30% and 40% more shoot fresh weight, respectively, relative to the wild-type (WT). Both transgenic lines showed higher tolerance to Cd, as evidenced by lower levels of electrolyte leakage and malondialdehyde compared to the WT plants under Cd stress. FaHSP17.8-CII overexpression increased shoot Cd contents 49-59% over the WT plants. The Cd translocation factor of root-to-shoot in OE grasses was 69-85% greater than WT under Cd stress. Furthermore, overexpression of FaHSP17.8-CII reduced Cd-induced damages of chloroplast ultra-structure and chlorophyll synthesis, and then improved photosystem II (PSII) function under Cd stress, which resulted in less reactive oxygen species (ROS) accumulation in OE grasses than that in WT exposed to Cd stress. The study suggests a novel FaHSP17.8-CII-PSII-ROS module to understand the mechanisms of Cd detoxification and tolerance, which provides a new strategy to improve phytoremediation efficiency in Cd-stressed grasses.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125932DOI Listing
May 2021

Stress memory gene FaHSP17.8-CII controls thermotolerance via remodeling PSII and ROS signaling in tall fescue.

Plant Physiol 2021 May 1. Epub 2021 May 1.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430074, China.

High-temperature is the most limiting factor in the growth of cool-season turfgrass. To cope with high-temperature stress, grass often adopt a memory response by remembering one past recurring stress and preparing a quicker and more robust reaction to the next stress exposure. However, little is known about how stress memory genes regulate the thermomemory response in cool-season turfgrass. Here, we characterized a transcriptional memory gene, Fa-heat shock protein 17.8 Class II (FaHSP17.8-CII) in a cool-season turfgrass species, tall fescue (Festuca arundinacea Schreb.). The thermomemory of FaHSP17.8-CII continued for more than four days and was associated with a high H3K4me3 level in tall fescue under heat stress (HS). Furthermore, heat acclimation or priming (ACC)-induced reactive oxygen species (ROS) accumulation and photosystem II (PSII) electron transport were memorable, and this memory response was controlled by FaHSP17.8-CII. In the fahsp17.8-CII mutant generated using CRISPR/Cas9, ACC+HS did not substantially block the ROS accumulation, the degeneration of chloroplast ultra-structure and the inhibition of PSII activity compared to HS alone. However, overexpression of FaHSP17.8-CII in tall fescue reduced ROS accumulation and chloroplast ultra-structure damage, and improved chlorophyll content and PSII activity under ACC+HS compared with that HS alone. These findings unveil a FaHSP17.8-CII-PSII-ROS module regulating transcriptional memory to enhance thermotolerance in cool-season turfgrass.
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http://dx.doi.org/10.1093/plphys/kiab205DOI Listing
May 2021

Discovery of indoline derivatives as anticancer agents via inhibition of tubulin polymerization.

Bioorg Med Chem Lett 2021 Jul 11;43:128095. Epub 2021 May 11.

School of Basic Medical Sciences, Zhengzhou University Zhengzhou 450001, China. Electronic address:

Human esophageal squamous cell carcinoma (ESCC) is one of the most lethal cancers in human digestive system. It is necessary to discover novel antitumor agents for the treatment of esophageal cancers because of its poor prognosis. Indoline has been reported as an efficient anticancer fragment to design novel anticancer agents. In this work, indoline derivatives were designed, synthesized and explored their anticancer activity. Compound 9d, which exhibited potent antiproliferative activity with IC values of 1.84 μM (MGC-803 cells), 6.82 μM (A549 cells), 1.61 μM (Kyse30 cells), 1.49 μM (Kyse450 cells), 2.08 μM (Kyse510 cells) and 2.24 μM (EC-109 cells), respectively. The most active compound 9d was identified as a tubulin inhibitor targeting colchicine binding site with an IC value of 3.4 µM. Compound 9d could strongly suppress the tubulin polymerization in Kyse450 cells. The results of molecular docking also suggested compound 9d could tightly bind into the colchicine binding site of tubulin. Besides, compound 9d inhibited the growth of KYSE450 cells in a time and dose-dependent manner. All the results suggest that the indoline derivatives may be a class of novel tubulin inhibitors with potential anticancer activity, and which is worthy of further study.
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http://dx.doi.org/10.1016/j.bmcl.2021.128095DOI Listing
July 2021

Semiparametric analysis of zero-inflated recurrent events with a terminal event.

Stat Med 2021 May 8. Epub 2021 May 8.

Global Statistical Sciences, Eli Lilly and Company, Indianapolis, Indiana.

Recurrent event data frequently arise in longitudinal studies and observations on recurrent events could be terminated by a major failure event such as death. In many situations, there exist a large fraction of subjects without any recurrent events of interest. Among these subjects, some are unsusceptible to recurrent events, while others are susceptible but have no recurrent events being observed due to censoring. In this article, we propose a zero-inflated generalized joint frailty model and a sieve maximum likelihood approach to analyze zero-inflated recurrent events with a terminal event. The model provides a considerable flexibility in formulating the effects of covariates on both recurrent events and the terminal event by specifying various transformation functions. In addition, Bernstein polynomials are employed to approximate the unknown cumulative baseline hazard (intensity) function. The estimation procedure can be easily implemented and is computationally fast. Extensive simulation studies are conducted and demonstrate that our proposed method works well for practical situations. Finally, we apply the method to analyze myocardial infarction recurrences in the presence of death in a clinical trial with cardiovascular outcomes.
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http://dx.doi.org/10.1002/sim.9013DOI Listing
May 2021

Promotes Proliferation and Metastasis by Targeting Hippo/YAP Signalling in Colorectal Cancer.

Front Cell Dev Biol 2021 15;9:656359. Epub 2021 Apr 15.

Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China.

The complex in which scribble planar cell polarity protein () is located is one of the three main polar protein complexes that play an important role in maintaining epithelial polarity and affecting tumour growth. However, the role of in colorectal cancer (CRC) remains largely unknown. This study used date from The Cancer Genome Atlas (TCGA) and clinical samples to determine the expression of in CRC and explored its mechanism through bioinformatics analysis and and experiments. In this study, was found to be highly expressed in CRC patients, and it was often associated with malignant characteristics, such as proliferation, apoptosis, and epithelial-mesenchymal transition (EMT). Furthermore, we found that may interact with the Hippo signalling pathway and affect the phosphorylation of YAP and its distribution inside and outside of the nucleus. We concluded that increased expression of is likely to inhibit the Hippo signalling pathway by promoting YAP phosphorylation. This role of in the progression of CRC provides an important information for the treatment of CRC.
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http://dx.doi.org/10.3389/fcell.2021.656359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084105PMC
April 2021

Three-in-one via syringe needle-based device: sampling, microextraction and peroxidase-like catalysis for colorimetric detection of the change of biogenic amines levels with time in meat.

Food Chem 2021 Oct 20;358:129900. Epub 2021 Apr 20.

School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China. Electronic address:

In this work, a syringe needle-based integrated method was designed for the detection of biogenic amines (BAs) in raw meat samples. Based on a sequential process, the needle-based sampling, micro liquid-phase extraction and peroxidase-like catalysis were adopted for the sample collection, target analytes extraction and colorimetric analysis, respectively. The proposed method exhibited high selectivity towards BAs (the total amount of histamine, putrescine and cadaverine was utilized to present the level of BAs), where the linear range is 5-50 μM and 50-1000 μM, and the limit of detection is 1.52 μM. Specifically, the whole process could be completed in a single syringe needle. In addition, due to the minimized sampling, the change of BAs levels with time in different area of real samples (fish) can be conveniently investigated. This method has the advantages of simplicity, low cost, high sensitivity and selectivity, endowing it a promising candidate for food analysis.
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http://dx.doi.org/10.1016/j.foodchem.2021.129900DOI Listing
October 2021

Simultaneous gene editing of three homoeoalleles in self-incompatible allohexaploid grasses.

J Integr Plant Biol 2021 Apr 29. Epub 2021 Apr 29.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, 430074, China.

CRISPR/Cas system has been widely used for precise gene editing in plants. However, simultaneous gene editing of multiple homoeoalleles remains challenging, especially in self-incompatible polyploid plants. Here, we simultaneously introduced targeted mutations in all three homoeoalleles of two genes in the self-incompatible allohexaploid tall fescue, using both CRISPR/Cas9 and LbCas12a (LbCpf1) systems. Loss-of-function mutants of FaPDS exhibited albino leaves, while knockout of FaHSP17.9 resulted in impaired heat resistance in T0 generation of tall fescue. Moreover, these mutations were inheritable. Our findings demonstrate the feasibility of generating loss-of-function mutants in a T0 generation polyploid perennial grasses using CRISPR/Cas systems. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13101DOI Listing
April 2021

The elevation of serum uric acid depends on insulin resistance but not fasting plasma glucose in hyperuricaemia.

Clin Exp Rheumatol 2021 Apr 15. Epub 2021 Apr 15.

Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, School of Medicine, Southeast University, Nanjing, and Shenzhen People's Hospital, The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China.

Objectives: The association between serum uric acid (SUA) and fasting plasma glucose (FPG) has not been fully outlined, in particular in hyperuricaemic population. This study aimed to address this issue, along with the exploration of the role of insulin resistance that was assessed by triglyceride-and-glucose (TyG) index.

Methods: A total of 16,297 participants without known diabetes from the SENSIBLE and SENSIBLE-Addition studies were included in the present analysis. Hyperuricaemia was defined as SUA ≥6 mg/dL. Generalised addictive model was applied to establish the relationship of SUA with FPG, and mediation analysis was performed to assess how insulin resistance affected the relationship.

Results: SUA showed an inverted U-shaped association with FPG, with the turning point of FPG at 6.1 mmol/L and 7.5 mmol/L in normouricaemic and hyperuricaemic participants, respectively. However, the significant relationship between SUA and FPG disappeared in hyperuricaemic participants (form B=3.3, 95% CI: 0.6-5.9, p=0.016 to B= -0.2, 95% CI: -3.1-2.7, p=0.894), and attenuated in normouricaemic participants (from B=9.8, 95% CI: 8.0-11.7, p<0.001 to B=7.3, 95% CI: 5.3-9.2, p<0.001) after controlling for TyG index. In the ascending segment, the relationship between SUA and FPG was partially mediated by TyG index in normouricaemic participants, but fully in hyperuricaemic participants.

Conclusions: SUA had an inverted U-shaped relationship with FPG, and their positive relationship was fully mediated by insulin resistance in participants with hyperuricaemia but not those without.
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April 2021

The mediating role of daytime sleepiness between problematic smartphone use and post-traumatic symptoms in COVID-19 home-refined adolescents.

Child Youth Serv Rev 2021 Jul 8;126:106012. Epub 2021 Apr 8.

Centre for Educational and Health Psychology, Sichuan University, Chengdu, China.

Background: COVID-19 was first recognized in late 2019 in China, at which time school closures forced most students to isolate at home or maintain social distance, both of which increased smartphone use, daytime sleepiness and post traumatic disorder (PTSD) risks. However, to date, no research has fully explored these behavioral risks or the consequences.

Methods: Two thousand and ninety home-confined students from two Chinese high schools participated in an online-based questionnaire battery that assessed their sociodemographic characteristics, COVID-19 related exposures, daytime sleepiness, problematic smartphone use, and PTSD. The subsequent data were subjected to mediation analysis, and structural equation models (SEM) were employed to explore the variable relationships.

Results: The problematic smartphone use, daytime sleepiness and PTSD prevalence were respectively 16.4%, 20.2% and 6.9%. The number of COVID-19 related exposure was directly associated with problematic smartphone use and PTSD symptoms. Problematic smartphone use was found to be a mediator between COVID-19 related exposure and PTSD symptoms, and daytime sleepiness was found to partially mediate the associations between problematic smartphone use and PTSD.

Conclusions: The more exposure associated with the pandemic, the more psychological and behavioral problems the adolescents had. The relatively high rate of problematic smartphone use in home isolated adolescents possibly increased the risk of daytime sleepiness and psychological problems. Therefore, targeted improvements are needed to reduce the risk of psychological problems and daytime sleepiness in adolescents.
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http://dx.doi.org/10.1016/j.childyouth.2021.106012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028598PMC
July 2021

Association between programmed cell death ligand 1 expression and thyroid cancer: A meta-analysis.

Medicine (Baltimore) 2021 Apr;100(14):e25315

Department of Nuclear Medicine.

Background: Programmed cell death ligand 1 (PD-L1), which is highly expressed in a variety of malignant tumors, is closely related to clinicopathological features and prognosis. However, there are few studies on the potential effects of PD-L1 on thyroid carcinoma, the incidence of which has shown an upward trend worldwide. This study aimed to explore the association between PD-L1 expression and clinicopathological features and prognosis of thyroid cancer.

Methods: An elaborate retrieval was performed using Medline, PubMed, Cochrane Library, EMBASE, Web of Science, WanFang databases, and China National Knowledge Infrastructure to determine the association between PD-L1 expression and disease-free survival (DFS), overall survival (OS), and clinicopathological features in patients with thyroid cancer. Study selection, data extraction, risk assessment, and data synthesis were performed independently by 2 reviewers. In this meta-analysis, RevMan 5.3 and Stata 15.1 were used for bias risk assessment and data synthesis.

Results: After a detailed search, 2546 cases reported in 13 articles were included in this meta-analysis. The outcomes revealed that high expression of PD-L1 in patients with thyroid cancer was associated with poor DFS (hazard ratio [HR] = 3.37, 95% confidence interval [CI] 2.54-4.48, P < .00001) and OS (HR = 2.52, 95% CI: 1.20-5.32, P = .01). High PD-L1 expression was associated with tumor size ≥2 cm, tumor recurrence, extrathyroidal extension, concurrent thyroiditis, unifocal tumor, and absence of psammoma body (P < .05). Subgroup analysis showed that positive expression of PD-L1 was related to poor prognosis for DFS of non-medullary thyroid carcinoma, and the overexpression of PD-L1 in differentiated thyroid carcinoma (DTC) was related to tumor recurrence, concurrent thyroiditis, extrathyroidal extension, unifocal DTC, late stage DTC, and BRAFV600E mutation in DTC.

Conclusion: PD-L1 is a significant predictor of prognosis and malignancy of thyroid cancer (especially DTC), and PD-L1 inhibitors may be a promising therapeutic option for refractory thyroid cancer in the future.
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http://dx.doi.org/10.1097/MD.0000000000025315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036129PMC
April 2021

The C4 protein encoded by Tomato leaf curl Yunnan virus interferes with mitogen-activated protein kinase cascade-related defense responses through inhibiting the dissociation of the ERECTA/BKI1 complex.

New Phytol 2021 07 4;231(2):747-762. Epub 2021 May 4.

State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310058, China.

Mitogen-activated protein kinase (MAPK) cascades are involved in host defense against pathogens and are often activated by upstream plasma membrane leucine-rich repeat receptor-like kinases (LRR-RLKs). ERECTA (ER) is an LRR-RLK that regulates plant developmental processes through activating MAPK cascades. Tomato leaf curl Yunnan virus (TLCYnV) C4 protein interacts with BKI1, stabilizes it at the plasma membrane and impairs ER autophosphorylation through suppressing the dissociation of the BKI1/ER complex, and then inhibits the activation of downstream MAPK cascades, which ultimately creates a favorable environment for TLCYnV infection. This study provides a novel viral strategy to impair MAPK activation.
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http://dx.doi.org/10.1111/nph.17387DOI Listing
July 2021

Li/Na substitution and Yb co-doping enabling tunable near-infrared emission in LiInSbO:Cr phosphors for light-emitting diodes.

iScience 2021 Apr 1;24(4):102250. Epub 2021 Mar 1.

State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and Devices, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.

Near-infrared (NIR) phosphor-converted light-emitting diode (pc-LED) has great potential in non-invasive detection, while the discovery of tunable broadband NIR phosphor still remains a challenge. Here, we report that Cr-activated LiInSbO exhibits a broad emission band ranging from 780 to 1400 nm with a full width at half maximum (FWHM) of 225 nm upon 492 nm excitation. The emission peaks are tuned from 970 to 1020 nm together with considerable broadening of FWHM (∼285 nm) via Li/Na substitution. Depending on Yb co-doping, a stronger NIR fluorescence peak of Yb appears with improved thermal resistance, which is ascribed to efficient energy transfer from Cr to Yb. An NIR pc-LED package has been finally designed and demonstrated a remarkable ability to penetrate pork tissues (∼2 cm) so that the insertion depth of a needle can be observed, indicating that the phosphor can be applied in non-destructive monitoring.
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http://dx.doi.org/10.1016/j.isci.2021.102250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995531PMC
April 2021

Complete mitochondrial genome and phylogenetic analysis of (Teleostei: Cypriniformes: Cyprinidae).

Mitochondrial DNA B Resour 2021 Mar 19;6(3):1118-1119. Epub 2021 Mar 19.

College of Life Sciences, Sichuan Normal University, Chengdu, China.

This study describes the first sequencing of the complete mitochondrial genome of , a species of cyprinid snowtrout from the Jialing River and Fujiang River basins in China's Sichuan Province. The total length is 16,571 base pairs. Similar to most Schizothoracinae mitochondrial genomes, there are 37 genes including 13 protein coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. In addition, it contains a control region rich in A-T nucleotides. The overall nucleotide composition is 29.6% for A, 27.1% for C, 17.9% for G and 25.4% for T, and the percentage of GC content is 45.0%. Phylogenetic analysis suggested that and clustered together in a clade. This work provides additional molecular information for studying conservation genetics and evolutionary relationships.
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http://dx.doi.org/10.1080/23802359.2021.1901622DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995835PMC
March 2021

Different Lipid Parameters in Predicting Clinical Outcomes in Chinese Statin-Naïve Patients After Coronary Stent Implantation.

Front Cardiovasc Med 2021 16;8:638663. Epub 2021 Mar 16.

Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, China.

Low-density lipoprotein cholesterol (LDL-C) is a critical surrogate outcome for cardiovascular disease (CVD). Recent observational studies identified different predictive lipid parameters, but these have not been fully validated in the Chinese population. This study aimed to compare the predictive value of lipid parameters for cardiovascular outcomes in Chinese statin-naïve patients who underwent percutaneous coronary intervention (PCI). We retrospectively recruited statin-naïve patients who underwent PCI for stable angina and acute coronary syndrome at Sichuan Provincial People's Hospital between 1 January 2016 and 31 December 2017. A follow-up was conducted via outpatient visits or telephone. We divided patients into three groups based on lipid parameter tertiles. We calculated the hazard ratios (HRs) of the highest and lowest tertiles for major adverse cardiovascular events (MACEs) using multivariate Cox proportional hazards regression. We compared the association strength of lipid parameters with MACEs using the HR of non-LDL-C lipid parameters relative to LDL-C. Among 445 included patients, the highest LDL-C, LDL-C/high-density lipoprotein cholesterol (HDL-C), atherosclerosis index, and non-HDL-C level tertiles were associated with an average increase of 165% (HR 2.65, confidence interval [CI] 1.26 to 5.61; = 0.01), 324% (HR 4.24, CI 1.89 to 9.52; < 0.001), 152% (HR 2.52, CI 1.22 to 5.22; = 0.01), and 125% (HR 2.25, CI 1.09 to 4.64; = 0.01) in the hazard of composite CVD, respectively. Lipoprotein (a) levels did not show a significant association with the endpoints. Except for LDL-C/HDL-C, different lipid parameter HR ratios were <1.0; none were statistically significant. Compared with non-LDL-C lipid parameters, LDL-C acts better predictive value for cardiovascular outcomes in general Chinese statin-naïve post-PCI patients.
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http://dx.doi.org/10.3389/fcvm.2021.638663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007761PMC
March 2021