Publications by authors named "Hu Li"

1,170 Publications

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FoxM1 insufficiency hyperactivates Ect2-RhoA-mDia1 signaling to drive cancer.

Nat Cancer 2020 Oct 12;1(10):1010-1024. Epub 2020 Oct 12.

Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, MN, USA.

FoxM1 activates genes that regulate S-G2-M cell-cycle progression and, when overexpressed, is associated with poor clinical outcome in multiple cancers. Here we identify FoxM1 as a tumor suppressor in mice that, through its N-terminal domain, binds to and inhibits Ect2 to limit the activity of RhoA GTPase and its effector mDia1, a catalyst of cortical actin nucleation. FoxM1 insufficiency impedes centrosome movement through excessive cortical actin polymerization, thereby causing the formation of non-perpendicular mitotic spindles that missegregate chromosomes and drive tumorigenesis in mice. Importantly, low expression correlates with RhoA GTPase hyperactivity in multiple human cancer types, indicating that suppression of the newly discovered Ect2-RhoAmDia1 oncogenic axis by FoxM1 is clinically relevant. Furthermore, by dissecting the domain requirements through which FoxM1 inhibits Ect2 GEF activity, we provide mechanistic insight for the development of pharmacological approaches that target protumorigenic RhoA activity.
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http://dx.doi.org/10.1038/s43018-020-00116-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623810PMC
October 2020

Ultrastructural Variations of Antennae and Labia Are Associated with Feeding Habit Shifts in Stink Bugs (Heteroptera: Pentatomidae).

Biology (Basel) 2021 Nov 10;10(11). Epub 2021 Nov 10.

Department of Entomology, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

The family Pentatomidae (stink bugs) is one of the largest groups in Heteroptera, containing many important pests and natural enemies. They exhibit highly diversified feeding habits and related structural modifications, but the key morphological characteristics associated with feeding habit radiation remain unclear. In the current study, we address this question by analyzing morphological variations of feeding related organs. We compare the ultrastructures of antennae and mouthparts across the chosen 17 species in Pentatomidae, representing both plant feeders and predators from four subfamilies. A strong association between ultrastructural adaptation and feeding habit transition has been revealed. The long, sharp, and hook-like mandibular teeth and maxillary barbs are exclusively present in predatory Pentatomidae, suggesting their tight association with the shift of feeding habit from phytophagy to predation. Significant differences between phytophagous and predatory species are also found in antennal and labial sensilla types and arrangements, implying their important function in food selection. Our data identify a series of key morphological structures associated with feeding habit variations among stink bugs, which will facilitate future studies on adaptive evolution of feeding habits, utilization, and population control of economic species in Pentatomidae as well as in other heteropteran lineages.
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http://dx.doi.org/10.3390/biology10111161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615146PMC
November 2021

Serum and glucocorticoid-regulated kinase 1 regulates transforming growth factor β1-connective tissue growth factor pathway in chronic rhinosinusitis.

Clin Immunol 2021 Nov 24;234:108895. Epub 2021 Nov 24.

Department of Otolaryngology-Head and Neck Surgery, Eye and ENT Hospital, Fudan University, No. 83, Fenyang Road, Xuhui District, Shanghai, China. Electronic address:

Purpose: Serum/glucocorticoid-regulated kinase 1 (SGK1) has been identified as a crucial regulator in fibrotic disorders. Herein, we explored SGK1 role in tissue remodeling of chronic rhinosinusitis (CRS).

Methods: Lentivirus was employed to generate an SGK1-overexpressing human bronchial epithelial cell (16HBE) line. To screen SGK1 downstream genes, RNA sequencing was performed on SGK1-overexpressing and control cell lines. To determine protein and gene expression levels, immunohistochemistry, western blotting, and quantitative real-time polymerase chain reaction were employed. Correlation analysis was performed using mRNA expression levels of SGK1, transforming growth factor β1 (TGF-β1), and connective tissue growth factor (CTGF) derived from CRS mucosal tissue and GEO database. Gene set enrichment analysis was conducted using gene sets from Molecular Signatures Database. The severity of symptoms in CRS patients was assessed using the 22-Item Sinonasal Outcome Test.

Results: SGK1 overexpression significantly increased the expression of connective tissue growth factor (CTGF) in 16HBE cells (P < 0.01). Consistently, CTGF protein level was considerably greater in mucosal tissue of CRS without nasal polyps (CRSsNP) than in CRS with nasal polyps (CRSwNP) (P < 0.05) or in control subjects (P < 0.01). TGF-β1 protein level was higher in mucosal tissue of CRSsNP patients than in CRSwNP patients (P < 0.001) or in the control group (P < 0.01). mRNA levels of SGK1 and CTGF (P < 0.05, r = 0.668; P = 0.001, r = 0.630), TGF-β1 and CTGF (P < 0.05, r = 0.560; P < 0.05, r = 0.420), as well as SGK1 and TGF-β1(P < 0.05, r = 0.612; P < 0.05, r = 0.524) were significantly correlated in CRS mucosal tissue and GSE36830 dataset, respectively. TGF-β1-induced upregulated genes were significantly enriched in SGK1 overexpression group. In vitro assays, TGF-β1 promoted SGK1 and CTGF expression in a concentration- and time-dependent manner. Administrating an SGK1 inhibitor, GSK650394, significantly inhibited TGF-β1-induced CTGF expression in 16HBE and dispersed primary nasal polyp cells.

Conclusions: TGF-β1 stimulation significantly increases SGK1 and CTGF expression. By regulating TGF-β1-CTGF pathway, SGK1 may participate in tissue remodeling in the pathological mechanism of CRS.
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http://dx.doi.org/10.1016/j.clim.2021.108895DOI Listing
November 2021

High Channel Temperature Mapping Electronics in a Thin, Soft, Wireless Format for Non-Invasive Body Thermal Analysis.

Biosensors (Basel) 2021 Nov 2;11(11). Epub 2021 Nov 2.

Department of Biomedical Engineering, City University of Hong Kong, Hong Kong 999077, China.

Hemodynamic status has been perceived as an important diagnostic value as fundamental physiological health conditions, including decisive signs of fatal diseases like arteriosclerosis, can be diagnosed by monitoring it. Currently, the conventional hemodynamic monitoring methods highly rely on imaging techniques requiring inconveniently large numbers of operation procedures and equipment for mapping and with a high risk of radiation exposure. Herein, an ultra-thin, noninvasive, and flexible electronic skin (e-skin) hemodynamic monitoring system based on the thermal properties of blood vessels underneath the epidermis that can be portably attached to the skin for operation is introduced. Through a series of thermal sensors, the temperatures of each subsection of the arrayed sensors are observed in real-time, and the measurements are transmitted and displayed on the screen of an external device wirelessly through a Bluetooth module using a graphical user interface (GUI). The degrees of the thermal property of subsections are indicated with a spectrum of colors that specify the hemodynamic status of the target vessel. In addition, as the sensors are installed on a soft substrate, they can operate under twisting and bending without any malfunction. These characteristics of e-skin sensors exhibit great potential in wearable and portable diagnostics including point-of-care (POC) devices.
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http://dx.doi.org/10.3390/bios11110435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615861PMC
November 2021

Exposure to duloxetine during pregnancy and risk of congenital malformations and stillbirth: A nationwide cohort study in Denmark and Sweden.

PLoS Med 2021 Nov 22;18(11):e1003851. Epub 2021 Nov 22.

Copenhagen Phase IV Unit (Phase4CPH), Department of Clinical Pharmacology and Center for Clinical Research and Prevention, Copenhagen University Hospital Bispebjerg and Frederiksberg, Copenhagen, Denmark.

Background: The prevalence of depression and the exposure to antidepressants are high among women of reproductive age and during pregnancy. Duloxetine is a selective serotonin-norepinephrine reuptake inhibitor (SNRI) approved in the United States and Europe in 2004 for the treatment of depression. Fetal safety of duloxetine is not well established. The present study evaluates the association of exposure to duloxetine during pregnancy and the risk of major and minor congenital malformations and the risk of stillbirths.

Methods And Findings: A population-based observational study was conducted based on data from registers in Sweden and Denmark. All registered births and stillbirths in the medical birth registers between 2004 and 2016 were included. Malformation diagnoses were identified up to 1 year after birth. Logistic regression analyses were used. Potential confounding was addressed through multiple regression, propensity score (PS) matching, and sensitivity analyses. Confounder variables included sociodemographic information (income, education, age, year of birth, and country), comorbidity and comedication, previous psychiatric contacts, and birth-related information (smoking during pregnancy and previous spontaneous abortions and stillbirths). Duloxetine-exposed women were compared with 4 comparators: (1) duloxetine-nonexposed women; (2) selective serotonin reuptake inhibitor (SSRI)-exposed women; (3) venlafaxine-exposed women; and (4) women exposed to duloxetine prior to, but not during, pregnancy. Exposure was defined as redemption of a prescription during the first trimester and throughout pregnancy for the analyses of malformations and stillbirths, respectively. Outcomes were major and minor malformations and stillbirths gathered from the national patient registers. The cohorts consisted of more than 2 million births with 1,512 duloxetine-exposed pregnancies. No increased risk for major malformations, minor malformations, or stillbirth was found across comparison groups in adjusted and PS-matched analyses. Duloxetine-exposed versus duloxetine-nonexposed PS-matched analyses showed odds ratio (OR) 0.98 (95% confidence interval [CI] 0.74 to 1.30, p = 0.909) for major malformations, OR 1.09 (95% CI 0.82 to 1.45, p = 0.570) for minor malformation, and 1.18 (95% CI 0.43 to 3.19, p = 0.749) for stillbirths. For the individual malformation subtypes, some findings were statistically significant but were associated with large statistical uncertainty due to the extremely small number of events. The main limitations for the study were that the indication for duloxetine and a direct measurement of depression severity were not available to include as covariates.

Conclusions: Based on this observational register-based nationwide study with data from Sweden and Denmark, no increased risk of major or minor congenital malformations or stillbirth was associated with exposure to duloxetine during pregnancy.
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http://dx.doi.org/10.1371/journal.pmed.1003851DOI Listing
November 2021

Innovative application of internal carotid artery embolization in salvage endoscopic nasopharyngectomy for recurrent nasopharyngeal carcinoma: A case-matched comparison.

Int Forum Allergy Rhinol 2021 Nov 13. Epub 2021 Nov 13.

ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.

Background: The efficacy and safety of internal carotid artery (ICA) embolization as a treatment strategy in recurrent nasopharyngeal carcinoma (rNPC) patients with tumors invading the ICA remain unclear.

Methods: We enrolled all rNPC patients with tumors invading the ICA, who underwent salvage endoscopic surgery. Using propensity scores to adjust for specific potential prognostic factors, a well-balanced cohort of 42 patients with limited rNPC was formed by matching each patient who underwent ICA embolization (study group) with one who did not (control group). The survival rates and common treatment-related complications were compared between the two groups.

Results: The cohort included patients with the following tumor stages: rT2 (n = 3), rT3 (n = 24), and rT4 (n = 15). During a median follow-up of 15 months (2-63), the 3-year overall survival and progression-free survival rates were significantly higher in the ICA embolization group than in the ICA non-embolization group (72.4% vs. 17.8% and 53.4% vs. 33.0%, respectively; P = 0.022 and P = 0.006, respectively). Additionally, the incidence of treatment-related complications such as nasal obstruction, nasopharyngeal hemorrhage, and nasopharyngeal necrosis was significantly lower in the ICA embolization group than in the non-embolization group (P = 0.001, P = 0.014, and P = 0.038, respectively).

Conclusions: The innovative application of ICA embolization in endoscopic surgery in patients with rNPC invading the ICA significantly improved patient survival and reduced the risk of treatment-related complications. Therefore, this may be a safe and effective method with potential to improve the outcomes in rNPC patients. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/alr.22927DOI Listing
November 2021

Individualized Prediction of Females' Empathic Concern from Intrinsic Brain Activity within General Network of State Empathy.

Cogn Affect Behav Neurosci 2021 Nov 8. Epub 2021 Nov 8.

Research Center of Brain and Cognitive Neuroscience, Liaoning Normal University, Dalian, China.

Empathy can be measured based on behavioral tasks and self-report scales, which have been used to characterize the state and trait empathy, respectively, in previous studies. The neural correlates of state empathy have been deeply investigated, whereas the association between trait empathy and brain activity remains unclear. Thus, this study employed multiple variate pattern analysis (MVPA) to explore whether intrinsic brain activity (IBA) within state-empathy-related regions was associated with trait empathy. Meta-analysis of empathy-related fMRI experiments identified a general network underlying state empathy, which is located in the bilateral supplementary motor area (SMA) extending to the middle cingulate cortex (MCC) and left anterior insula (AI) and extending to the inferior frontal gyrus (IFG). The subsequent MVPA found that empathic concern can be predicted through the IBA of the general network at the female individual level (i.e., the fractional amplitude of low-frequency fluctuations and regional homogeneity). Based on the resting state fMRI (rs-fMRI), these results further support the involvement of SMA/MCC and AI/IFG in empathy. Meanwhile, the significant predictive association between IBA and trait empathy offers new insights into the general component of empathy, which may indicate the potential of using rs-fMRI to achieve the objective measurement of empathic ability.
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http://dx.doi.org/10.3758/s13415-021-00964-zDOI Listing
November 2021

Senescent cells suppress innate smooth muscle cell repair functions in atherosclerosis.

Nat Aging 2021 Aug 2;1(8):698-714. Epub 2021 Aug 2.

Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester MN, United States.

Senescent cells (SNCs) degenerate the fibrous cap that normally prevents atherogenic plaque rupture, a leading cause of myocardial infarction and stroke. Here we explored the underlying mechanism using pharmacological or transgenic approaches to clear SNCs in the mouse model of atherosclerosis. SNC clearance reinforced fully deteriorated fibrous caps in highly advanced lesions, as evidenced by restored vascular smooth muscle cell (VSMC) numbers, elastin content, and overall cap thickness. We found that SNCs inhibit VSMC promigratory phenotype switching in the first interfiber space of the arterial wall directly beneath atherosclerotic plaque, thereby limiting lesion entry of medial VSMCs for fibrous cap assembly or reinforcement. SNCs do so by antagonizing IGF-1 through the secretion of insulin-like growth factor-binding protein 3 (Igfbp3). These data indicate that the intermittent use of senolytic agents or IGFBP-3 inhibition in combination with lipid lowering drugs may provide therapeutic benefit in atherosclerosis.
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http://dx.doi.org/10.1038/s43587-021-00089-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570576PMC
August 2021

Effect of Epidural Dexmedetomidine as an Adjuvant to Local Anesthetics for Labor Analgesia: A Meta-Analysis of Randomized Controlled Trials.

Evid Based Complement Alternat Med 2021 27;2021:4886970. Epub 2021 Oct 27.

Department of Anesthesia, Sichuan Jinxin Women and Children Hospital, Chengdu 610000, China.

Background: This study aims to determine the analgesic effect and safety of dexmedetomidine as an adjuvant to epidural local anesthetics during labor.

Methods: Randomized controlled trials comparing epidural blocks with or without dexmedetomidine for labor analgesia were comprehensively searched. Review manager 5.4 was used to analyze the extracted data.

Results: Compared with placebo and opioids, dexmedetomidine relieved labor pain of 15 min (=0.002), 30 min (=0.01), and 120 min (=0.02) after block and at the moment of fetal disengagement (=0.0002), decreased mean arterial pressure of 120 min (=0.01), heart rate of 30 min (=0.003), 60 min ( < 0.00001), and 120 min ( < 0.00001) after block, blood loss (=0.02), and the incidence of nausea/vomiting (=0.006), and increased the incidence of maternal bradycardia (=0.04). However, sensitivity analysis only found that the incidence of nausea/vomiting was significantly different. Compared with placebo, dexmedetomidine relieved labor pain of 30 min after block ( < 0.00001) and did not increase the incidences of side effects, but only two studies were enrolled. Compared with opioids, dexmedetomidine decreased the incidence of nausea/vomiting (=0.002), increased the incidence of maternal bradycardia (=0.04), and had a similar effect on labor pain relief; however, sensitivity analysis found that significant difference existed only at the incidence of nausea/vomiting. Other outcomes from meta-analysis or subgroup analysis were not different.

Conclusions: Epidural dexmedetomidine has the potential to offer a better analgesic effect than placebo, similar labor pain control to opioids, and has no definite adverse effects on the parturient or fetus, but more high-quality studies are needed to confirm these conclusions.
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http://dx.doi.org/10.1155/2021/4886970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568549PMC
October 2021

The role of negative emotions in sex differences in pain sensitivity.

Neuroimage 2021 Nov 3;245:118685. Epub 2021 Nov 3.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Pain perception varies widely among individuals due to the varying degrees of biological, psychological, and social factors. Notably, sex differences in pain sensitivity have been consistently observed in various experimental and clinical investigations. However, the neuropsychological mechanism underlying sex differences in pain sensitivity remains unclear. To address this issue, we quantified pain sensitivity (i.e., pain threshold and tolerance) using the cold pressure test and negative emotions (i.e., pain-related fear, pain-related anxiety, trait anxiety, and depression) using well-established questionnaires and collected magnetic resonance imaging (MRI) data (i.e., high-resolution T1 structural images and resting-state functional images) from 450 healthy subjects. We observed that, as compared to males, females exhibited lower pain threshold and tolerance. Notably, sex differences in pain sensitivity were mediated by pain-related fear and anxiety. Specifically, pain-related fear and anxiety were the complementary mediators of the relationship between sex and pain threshold, and they were the indirect-only mediators of the relationship between sex and pain tolerance. Besides, structural MRI data revealed that the amygdala subnuclei (i.e., the lateral and basal nuclei in the left hemisphere) volumes were the complementary mediators of the relationship between sex and pain-related fear, which further influenced pain sensitivity. Altogether, our results provided a comprehensive picture of how negative emotions (especially pain-related negative emotions) and related brain structures (especially the amygdala) contribute to sex differences in pain sensitivity. These results deepen our understanding of the neuropsychological underpinnings of sex differences in pain sensitivity, which is important to tailor a personalized method for treating pain according to sex and the level of pain-related negative emotions for patients with painful conditions.
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http://dx.doi.org/10.1016/j.neuroimage.2021.118685DOI Listing
November 2021

Efficacy of Acupuncture Combined with Rehabilitation Training for Intensive Care Unit-Acquired Muscle Weakness: A Protocol for a Randomized, Sham-Procedure-Controlled Clinical Trial.

Evid Based Complement Alternat Med 2021 20;2021:3539651. Epub 2021 Oct 20.

Department of Acupuncture-Moxibustion, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

To evaluate the efficacy of acupuncture combined with rehabilitation training in patients with intensive care unit (ICU)-acquired muscle weakness (ICUAW), a single-blinded, randomized, sham-controlled clinical trial is designed for execution. In total, 56 participants with ICUAW will be randomly assigned to the treatment and control groups with 28 participants in each group. The participants will be treated with acupunctures or sham procedures at LI15, LI11, ST36, GB34, and ST31, 5 times per week for a total of 20 sessions in 4 weeks while they will receive rehabilitation training. Patients will be followed up every month for 3 months after treatment. The primary outcomes include changes in quadriceps femoris muscle area, thickness, vastus intermediate muscle thickness, subcutaneous tissue thickness, and ultrasonic intensities of the rectus femoris. The secondary outcomes consist of the modified Barthel index score and the Medical Research Council total score. Participants' mechanical ventilation, the rate of detachment at the second week, the 28-day survival rate, and the occurrence of adverse reactions will be measured, and any side effects will be reported and recorded. Patient outcomes between the treatment and control groups will be compared and statistically tested. We anticipate that the therapeutic regimen of acupuncture combined with rehabilitation training would be more effective than the rehabilitation training alone for the treatment of the ICUAW. The findings of this study could help develop a better strategy for the treatment of the ICUAW disease and explore a clinical application of an acupuncture technique. Trial registration: Chinese Clinical Trial Register ChiCTR2000038779. Registered 30 September, 2020, https://www.chictr.org.cn/showproj.aspx?proj=62284.
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http://dx.doi.org/10.1155/2021/3539651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550834PMC
October 2021

Photoluminescent Semiconducting Graphene Nanoribbons via Longitudinally Unzipping Single-Walled Carbon Nanotubes.

ACS Appl Mater Interfaces 2021 Oct 30. Epub 2021 Oct 30.

Department of Electrical and Electronic Engineering, University of Manchester, M13 9PL Manchester, U.K.

The lack of a sizeable band gap has so far prevented graphene from building effective electronic and optoelectronic devices despite its numerous exceptional properties. Intensive theoretical research reveals that a band gap larger than 1 eV can only be achieved in sub-3 nm wide graphene nanoribbons (GNRs), but real fabrication of such ultranarrow GNRs still remains a critical challenge. Herein, we demonstrate an approach for the synthesis of ultranarrow and photoluminescent semiconducting GNRs by longitudinally unzipping single-walled carbon nanotubes. Atomic force microscopy reveals the unzipping process, and the resulting 2.2 nm wide GNRs are found to emit strong and sharp photoluminescence at ∼685 nm, demonstrating a very desirable semiconducting nature. This band gap of 1.8 eV is further confirmed by follow-up photoconductivity measurements, where a considerable photocurrent is generated, as the excitation wavelength becomes shorter than 700 nm. More importantly, our fabricated GNR field-effect transistors (FETs), by employing the hexagonal boron nitride-encapsulated heterostructure to achieve edge-bonded contacts, demonstrate a high current on/off ratio beyond 10 and carrier mobility of 840 cm/V s, approaching the theoretical scattering limit in semiconducting GNRs at room temperature. Especially, highly aligned GNR bundles with lengths up to a millimeter are also achieved by prepatterning a template, and the fabricated GNR bundle FETs show a high on/off ratio reaching 10, well-defined saturation currents, and strong light-emitting properties. Therefore, GNRs produced by this method open a door for promising applications in graphene-based electronics and optoelectronics.
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http://dx.doi.org/10.1021/acsami.1c14597DOI Listing
October 2021

Sustainable catalyst-free N-formylation using CO2 as a carbon source.

Curr Org Synth 2021 Oct 22. Epub 2021 Oct 22.

State Key Laboratory Breeding Base of Green Pesticide & Agricultural Bioengineering, Key Laboratory of Green Pesticide & Agricultural Bioengineering, Ministry of Education, State-Local Joint Laboratory for Comprehensive Utilization of Biomass, Center for R&D of Fine Chemicals, Guizhou University, Guiyang550025, Guizhou. China.

The development of new sustainable catalytic conversion methods of carbon dioxide (CO2) is of great interest in the synthesis of valuable chemicals. N-formylation of CO2 with amine nucleophiles as substrates has been studied in depth. The key to benign formylation is to select a suitable reducing agent to activate CO2. This paper showcases the activation modes of CO2 and the construction strategies of sustainable and catalyst-free N-formylation systems. The research progress of catalyst-free N-formylation of amines and CO2 is reviewed. There are two broad prominent categories, namely reductive amidation of CO2 facilitated by organic solvents and ionic liquids in the presence of hydrosilane. Attention is also paid to discussing the involved reaction mechanism with practical applications and identifying the remaining challenges in this field.
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http://dx.doi.org/10.2174/1570179418666211022160149DOI Listing
October 2021

Intensive Care Nurses' Knowledge, Attitude, and Practice of Pressure Injury Prevention in China: A Cross-Sectional Study.

Risk Manag Healthc Policy 2021 11;14:4257-4267. Epub 2021 Oct 11.

Faculty of Nursing, Prince of Songkla University, Hatyai, Songkhla, Thailand.

Background: Pressure injury (PI) remains a critical health issue worldwide. The global incidence of hospital-acquired PI is 8.4%, and among intensive-care unit (ICU) patients, it is even higher, ranging from 6.60% to 36.80%. It is important to investigate ICU nurses' PI prevention knowledge, attitudes, and practices (KAP).

Aim: The aim of this study was to describe the level of Chinese ICU nurses' knowledge, attitudes, and self-reported practices of PI prevention.

Materials And Methods: From March 31 to April 30, 2019, we recruited a total of 510 ICU nurses into the current study using convenience sampling. The Modified Pressure Ulcer Knowledge Assessment Tool version 2.0, Attitudes toward Pressure Ulcer Prevention instrument, and Questionnaire to evaluate nurses' Adherence to Recommendations for Preventing Pressure Ulcers were used for data collection. We used one-way analysis of variance, Student's -test, and the Mann-Whitney -test to assess differences in knowledge, attitudes, and self-reported practices of PI prevention among ICU nurses, whom we categorized by their demographic characteristics.

Results: Mean scores of participants' knowledge, attitude, and their self-report practice were 65.82 ± 9.29, 76.65 ± 8.62, and 83.35 ± 13.55, respectively. Participants with bachelor's degrees or higher, who worked in tertiary hospitals, and who had received training on PI prevention over the last year had higher scored on PI prevention knowledge (all < 0.05). Participants who had received training on PI prevention over the last year scored higher on attitude than those who had not received such training over the last year ( = 0.001).

Conclusion: ICU nurses surveyed in this study did not demonstrate an acceptable level of knowledge about PI prevention, while their attitudes and self-reported practices were acceptable. Continuing education or in-service training should be provided to enhance ICU nurses' knowledge of PI prevention.
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http://dx.doi.org/10.2147/RMHP.S323839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524251PMC
October 2021

Feasibility of standardized procedures of white light gastroscopy for clinical practice: A multicenter study in China.

J Dig Dis 2021 Nov;22(11):656-662

Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Objective: We aimed to establish a standardized procedure for white light gastroscopy (WLG) to screen gastric lesions including early gastric cancer (EGC) in China and to verify its efficacy and feasibility in clinical practice.

Methods: A standardized WLG procedure for outpatients at nine tertiary hospitals in Beijing was established. Clinical information of the participants and details of the endoscopic procedures were recorded.

Results: A total of 1051 participants were enrolled in a baseline conventional endoscopic survey between March 2014 and December 2015, while 2156 patients were enrolled in the standardized WLG operation from January 2016 to June 2017. The procedure time of the standardized procedure was significantly longer than that of the baseline conventional procedure (P = 0.003). More images were obtained during the standardized procedure compared with the baseline conventional procedure (P < 0.001). The overall detection rate of gastric lesions in the standardized procedure group was significantly higher than that in the baseline procedure group (52.5% vs 38.4%, P < 0.01). The satisfaction scores of both participants and endoscopists in the standardized procedure group were significantly higher than in the baseline procedure group.

Conclusions: Compared with the conventional procedure, standardized WLG procedure significantly improves the detection rate of gastric lesions as well as the satisfaction score of participants and endoscopists despite its longer procedure time. It is effective and feasible in clinical practice in China for the use of currently available endoscopic equipment.
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http://dx.doi.org/10.1111/1751-2980.13061DOI Listing
November 2021

Hierarchical Porous MIL-101(Cr) Solid Acid-Catalyzed Production of Value-Added Acetals from Biomass-Derived Furfural.

Polymers (Basel) 2021 Oct 12;13(20). Epub 2021 Oct 12.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide &Agricultural Bioengineering, Ministry of Education, State-Local Joint Laboratory for Comprehensive Utilization of Biomass, Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, China.

Considering economic and environmental impacts, catalytic biomass conversion to valuable compounds has attracted more and more attention. Of particular interest is furfural, a versatile biorefinery platform molecule used as a feedstock for the production of fuels and fine chemicals. In this study, the Cr-based metal-organic frameworks (MOFs) MIL-101 were modified by chlorosulfonic acid, and MIL-101 was changed into a hierarchical MOF structure with smaller particles and lower particle crystallinity by CTAB, which significantly improved the acidic sites of the MOFs. The original and modified MIL-101(Cr) catalysts were characterized by XRD, N adsorption-desorption, SEM, TEM, and FT-IR. The effects of different catalysts, reaction temperature, catalyst amount, and alcohol type on the reaction were studied. Under the action of the MOFs catalyst, a new mild route for the condensation of furfural with various alkyl alcohols to the biofuel molecules (acetals) was proposed. The conversion route includes the conversion of furfural up to 91% yield of acetal could be obtained within 1 h solvent-free and in room-temperature reaction conditions. The sulfonic acid-functionalized MIL-101(Cr) is easy to recover and reuse, and can still maintain good catalytic activity after ten runs.
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http://dx.doi.org/10.3390/polym13203498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539236PMC
October 2021

Selenomethionine alleviated Ochratoxin A induced pyroptosis and renal fibrotic factors expressions in MDCK cells.

J Biochem Mol Toxicol 2021 Oct 21:e22933. Epub 2021 Oct 21.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Ochratoxin A (OTA) is universally known to induce nephrotoxicity via inducing oxidative stress and apoptosis, inhibiting protein synthesis and activating autophagy. Our previous studies have proved that OTA induces nephrotoxicity in vitro and in vivo by adjusting the NOD-like receptor protein 3 (NLRP3) inflammasome activation and caspase-1-dependent pyroptosis. Based on these findings, we further investigated the protective role of selenomethionine (SeMet) on OTA-caused nephrotoxicity using the Madin-Darby canine kidney (MDCK) epithelial cells as an in vitro model, proposing to offer a new way for remedying OTA-induced nephrotoxicity by nutritional manipulation. We measured the cell vitality, lactate dehydrogenase (LDH) activity and the expression of renal fibrotic genes, NLRP3 inflammasome and pyroptosis related genes. MTT and LDH results indicated that SeMet supplementation significantly mitigated 2.0 μg/ml OTA-induced cytotoxicity in MDCK cells (p < 0.05). Meanwhile, SeMet alleviated OTA induced increase of reactive oxygen species in MDCK cells. Then, the expressions of α-SMA, Vimentin, and TGF-β were detected both in mRNA and protein levels. The results indicated 8 μM SeMet supplementation could significantly downregulate the expression of OTA-induced renal fibrosis-related genes (p < 0.05). In addition, the upregulation of OTA-induced NLRP3 inflammasome and pyroptosis downstream genes was also significantly inhibited by 8 μM of SeMet (p < 0.05). In summary, SeMet could alleviate OTA-induced renal fibrotic genes expression and reduce NLRP3-caspase-1-dependent pyroptosis. Therefore, SeMet supplementation may become an effective approach for preserving animals from renal injury exposed to OTA.
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http://dx.doi.org/10.1002/jbt.22933DOI Listing
October 2021

Anastrozole regulates fatty acid synthase in breast cancer.

Mol Cancer Ther 2021 Oct 19. Epub 2021 Oct 19.

Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic

Our previous matched case-control study of postmenopausal women with resected early stage breast cancer revealed that only anastrozole, but not exemestane or letrozole, showed a significant association between the six-month estrogen concentrations and risk of breast cancer event. Anastrozole, but not exemestane or letrozole, is a ligand for estrogen receptor α (ERα). The mechanisms of endocrine resistance are heterogenous and with the new mechanism of anastrozole, we have found that treatment of anastrozole maintains fatty acid synthase (FASN) protein level by limiting the ubiquitin-mediated FASN degradation, leading to increased breast cancer cell growth. Mechanistically, anastrozole decreases guided entry of tail-anchored proteins factor 4 (GET4) expression, resulting in decreased BCL2-associated athanogene cochaperone 6 (BAG6) complex activity, which in turn, prevents RNF126-mediated degradation of FASN. Increased FASN protein level can induce a negative feedback loop mediated by the mitogen-activated protein kinase (MAPK) pathway. High levels of FASN are associated with poor outcome only in anastrozole-treated breast cancer patients, but not in patients treated with exemestane or letrozole. Repressing FASN causes regression of breast cancer cell growth. The anastrozole-FASN signaling pathway is eminently targetable in endocrine-resistant breast cancer.
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http://dx.doi.org/10.1158/1535-7163.MCT-21-0509DOI Listing
October 2021

Metabolic remodelling during early mouse embryo development.

Nat Metab 2021 10 14;3(10):1372-1384. Epub 2021 Oct 14.

Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University, Hangzhou, China.

During early mammalian embryogenesis, changes in cell growth and proliferation depend on strict genetic and metabolic instructions. However, our understanding of metabolic reprogramming and its influence on epigenetic regulation in early embryo development remains elusive. Here we show a comprehensive metabolomics profiling of key stages in mouse early development and the two-cell and blastocyst embryos, and we reconstructed the metabolic landscape through the transition from totipotency to pluripotency. Our integrated metabolomics and transcriptomics analysis shows that while two-cell embryos favour methionine, polyamine and glutathione metabolism and stay in a more reductive state, blastocyst embryos have higher metabolites related to the mitochondrial tricarboxylic acid cycle, and present a more oxidative state. Moreover, we identify a reciprocal relationship between α-ketoglutarate (α-KG) and the competitive inhibitor of α-KG-dependent dioxygenases, L-2-hydroxyglutarate (L-2-HG), where two-cell embryos inherited from oocytes and one-cell zygotes display higher L-2-HG, whereas blastocysts show higher α-KG. Lastly, increasing 2-HG availability impedes erasure of global histone methylation markers after fertilization. Together, our data demonstrate dynamic and interconnected metabolic, transcriptional and epigenetic network remodelling during early mouse embryo development.
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http://dx.doi.org/10.1038/s42255-021-00464-xDOI Listing
October 2021

Sensitivity of SARS-CoV-2 Variants to Neutralization by Convalescent Sera and a VH3-30 Monoclonal Antibody.

Front Immunol 2021 23;12:751584. Epub 2021 Sep 23.

Institute of Immunology, Third Military Medical University, Chongqing, China.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic of novel coronavirus disease (COVID-19). Though vaccines and neutralizing monoclonal antibodies (mAbs) have been developed to fight COVID-19 in the past year, one major concern is the emergence of SARS-CoV-2 variants of concern (VOCs). Indeed, SARS-CoV-2 VOCs such as B.1.1.7 (UK), B.1.351 (South Africa), P.1 (Brazil), and B.1.617.1 (India) now dominate the pandemic. Herein, we found that binding activity and neutralizing capacity of sera collected from convalescent patients in early 2020 for SARS-CoV-2 VOCs, but not non-VOC variants, were severely blunted. Furthermore, we observed evasion of SARS-CoV-2 VOCs from a VH3-30 mAb 32D4, which was proved to exhibit highly potential neutralization against wild-type (WT) SARS-CoV-2. Thus, these results indicated that SARS-CoV-2 VOCs might be able to spread in convalescent patients and even harbor resistance to medical countermeasures. New interventions against these SARS-CoV-2 VOCs are urgently needed.
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http://dx.doi.org/10.3389/fimmu.2021.751584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495157PMC
October 2021

Association between 15 known or potential breast cancer susceptibility genes and breast cancer risks in Chinese women.

Cancer Biol Med 2021 Oct 5. Epub 2021 Oct 5.

Department of Breast Surgery, Peking University International Hospital, Beijing 102206, China.

Objective: There are many hereditary breast cancer patients in China, and multigene panel testing has been a new paradigm of genetic testing for these patients and their relatives. However, the magnitude of breast cancer risks related to multiple breast cancer susceptibility genes are largely unknown in Chinese women.

Methods: We screened pathogenic variants in 15 established or potential breast cancer susceptibility genes from 8,067 consecutive Chinese female breast cancer patients and 13,129 Chinese cancer-free female controls. These breast cancer patients were unselected for age at diagnosis or family history.

Results: We found that pathogenic variants in [odds ratio (OR): 16.9, 95% confidence interval (CI): 5.2-55.2]; (OR: 10.4, 95% CI: 7.6-14.2); (OR: 9.7, 95% CI: 6.3-14.8); and (OR: 5.2, 95% CI: 3.0-8.8) were associated with a high risk of breast cancer. , , , and were associated with a moderate risk of breast cancer with ORs ranging from 2-fold to 4-fold. In contrast, pathogenic variants of , , , and were not associated with increased risk of breast cancer in Chinese women. The pathogenic variants of , , and were very rare, so they had a limited contribution to Chinese breast cancer. Patients with pathogenic variants of , , , and more often had early-onset breast cancer, bilateral breast cancer, and a family history of breast cancer and/or any cancer.

Conclusions: This study provided breast cancer risk assessment data for multiple genes in Chinese women, which is useful for genetic testing and clinical management of Chinese hereditary breast cancer.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2021.0358DOI Listing
October 2021

Sigma-1 Receptor Activation Suppresses Microglia M1 Polarization via Regulating Endoplasmic Reticulum-Mitochondria Contact and Mitochondrial Functions in Stress-Induced Hypertension Rats.

Mol Neurobiol 2021 Oct 2. Epub 2021 Oct 2.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.

Exposure to stress plays a detrimental role in the pathogenesis of hypertension via neuroinflammation pathways. Microglial neuroinflammation in the rostral ventrolateral medulla (RVLM) exacerbates stress-induced hypertension (SIH) by increasing sympathetic hyperactivity. Mitochondria of microglia are the regulators of innate immune response. Sigma-1R (σ-1R) localizes to the mitochondria-associated membranes (MAMs) and regulates endoplasmic reticulum (ER) and mitochondria communication, in part through its chaperone activity. The present study aims to investigate the protective role of σ-1R on microglial-mediated neuroinflammation. Stress-induced hypertension (SIH) was induced in rats using electric foot shocks and intermittent noise. Arterial blood pressure (ABP), heart rate (HR), and renal sympathetic nerve activity (RSNA) were measured to evaluate the sympathetic nervous system (SNS) activities. SKF10047 (100 µM), an agonist of σ-1R, was administrated to rats, then σ-1R localization and MAM alterations were detected by immuno-electron microscopy. Mitochondrial calcium homeostasis was examined in primary microglia and/or BV-2 microglia cells. The effect of SKF10047 treatment on the mitochondrial respiratory function of cultured microglia was measured using a Seahorse Extracellular Flux Analyzer. Confocal microscopic images were performed to indicate mitochondrial dynamics. Stress reduces σ-1R's localization at the MAMs, leading to decreased ER-mitochondria contact and IP3R-GRP75-VDAC calcium transport complexes expression in the RVLM of rats. SKF10047 promotes the length and coverage of MAMs in the prorenin-treated microglia. Prorenin treatment increases mitoROS levels, and inhibits Ca signalling between the two organelles, therefore negatively affects ATP production in BV2 cells, and these effects are reversed by SKF10047 treatment. We found mitochondrial hyperfusion and microglial M1 polarization in prorenin-treated microglia. SKF10047 suppresses microglial M1 polarization and RVLM neuroinflammation, subsequently ameliorates sympathetic hyperactivity in stress-induced hypertensive rats. Sigma-1 receptor activation suppresses microglia M1 polarization and neuroinflammation via regulating endoplasmic reticulum-mitochondria contact and mitochondrial functions in stress-induced hypertension rats.
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http://dx.doi.org/10.1007/s12035-021-02488-6DOI Listing
October 2021

Manifold medicine: A schema that expands treatment dimensionality.

Drug Discov Today 2021 Sep 29. Epub 2021 Sep 29.

Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic College of Medicine and Science, Rochester, MN 55905, USA. Electronic address:

Drug discovery currently focuses on identifying new druggable targets and drug repurposing. Here, we illustrate a third domain of drug discovery: the dimensionality of treatment regimens. We formulate a new schema called 'Manifold Medicine', in which disease states are described by vectorial positions on several body-wide axes. Thus, pathological states are represented by multidimensional 'vectors' that traverse the body-wide axes. We then delineate the manifold nature of drug action to provide a strategy for designing manifold drug cocktails by design using state-of-the-art biomedical and technological innovations. Manifold Medicine offers a roadmap for translating knowledge gained from next-generation technologies into individualized clinical practice.
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http://dx.doi.org/10.1016/j.drudis.2021.09.016DOI Listing
September 2021

Cysteine-rich intestinal protein 1 enhances the progression of hepatocellular carcinoma via Ras signaling.

Authors:
Hong-Yu He Li Hu

Kaohsiung J Med Sci 2021 Sep 29. Epub 2021 Sep 29.

Physical Examination Center, Tai'an Medical District, 960 Hospital of PLA, Tai'an, China.

The present study aimed to explore the expression and clinical significance of cysteine-rich intestinal protein 1 (CRIP1) mRNA in the serum of patients with hepatocellular carcinoma (HCC). Reverse transcription polymerase chain reaction (RT-PCR) was performed to explore the level of CRIP1 mRNA in the tissues and serum of patients with HCC. Our data showed that the mRNA level of CRIP1 was significantly elevated in the serum and tissues of HCC patients. Moreover, serum CRIP1 mRNA was significantly elevated in HCC patients with larger tumor sizes and higher tumor node metastasis (TNM) stages. Receiver operating characteristic analysis showed that compared with a single marker, the combined detection of alpha-fetoprotein, carcinoembryonic antigen, and CRIP1 had the highest accuracy, sensitivity, and specificity. Further study showed that the overexpression of CRIP1 enhanced the proliferation and migration of HepG2 cells, but the inhibition of CRIP1 decreased the proliferation and migration of HepG2 cells. Microarray assays and KyotoEncyclopedia of Genes and Genomes (KEGG) pathway analysis showed that overexpression of CRIP1 induced the activation of Ras signaling. Co-immunoprecipitation (Co-IP) assays indicated that CRIP1 could interact with Ras. To further evaluate whether CRIP1 interacts with Ras, a specific siRNA targeting Ras was selected. We found that Ras knockdown reduced the activation of Ras/AKT signaling even in HepG2 cells transfected with CRIP1. Moreover, elevated expression of CRIP1 increased the proliferation of HepG2 cells, but such effects could be abolished by silencing Ras. In summary, elevated CRIP1 levels enhanced the progression of CRIP1 via Ras signaling.
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http://dx.doi.org/10.1002/kjm2.12445DOI Listing
September 2021

Multi-species single-cell transcriptomic analysis of ocular compartment regulons.

Nat Commun 2021 09 28;12(1):5675. Epub 2021 Sep 28.

Cell Fate Engineering and Therapeutics Laboratory, A*STAR Institute of Molecular and Cell Biology, Singapore, 138673, Singapore.

The retina is a widely profiled tissue in multiple species by single-cell RNA sequencing studies. However, integrative research of the retina across species is lacking. Here, we construct the first single-cell atlas of the human and porcine ocular compartments and study inter-species differences in the retina. In addition to that, we identify putative adult stem cells present in the iris tissue. We also create a disease map of genes involved in eye disorders across compartments of the eye. Furthermore, we probe the regulons of different cell populations, which include transcription factors and receptor-ligand interactions and reveal unique directional signalling between ocular cell types. In addition, we study conservation of regulons across vertebrates and zebrafish to identify common core factors. Here, we show perturbation of KLF7 gene expression during retinal ganglion cells differentiation and conclude that it plays a significant role in the maturation of retinal ganglion cells.
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http://dx.doi.org/10.1038/s41467-021-25968-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478974PMC
September 2021

Design, Synthesis, and Structure-Activity Relationship Studies of Magnolol Derivatives as Antifungal Agents.

J Agric Food Chem 2021 Oct 28;69(40):11781-11793. Epub 2021 Sep 28.

School of Pharmacy, Lanzhou University, Lanzhou 730000, People's Republic of China.

Plant pathogenic fungi seriously affect agricultural production and are difficult to control. The discovery of new leads based on natural products is an important way to innovate fungicides. In this study, 30 natural-product-based magnolol derivatives were synthesized and characterized on the basis of NMR and mass spectroscopy. Bioactivity tests on phytopathogenic fungi (, , , and ) in vitro of these compounds were performed systematically. The results showed that 11 compounds were active against four kinds of phytopathogenic fungi with EC values in the range of 1.40-20.00 μg/mL, especially compound that exhibited excellent antifungal properties against with an EC value of 2.86 μg/mL, approximately 2.8-fold more potent than magnolol (EC = 8.13 μg/mL). Moreover, compound showed the highest antifungal activity against and with EC values of 4.39 and 1.40 μg/mL, respectively, and compound showed good antifungal activity against . Then, an in vivo experiment of compound against was further investigated in vivo using infected tomatoes (curative effect, 50/200 and 36%/100 μg/mL). The physiological and biochemical studies illustrated that the primary action mechanism of compound on might change the mycelium morphology, increase cell membrane permeability, and destroy the function of mitochondria. Furthermore, structure-activity relationship (SAR) studies revealed that hydroxyl groups play a key role in antifungal activity. To sum up, this study provides a reference for understanding the application of magnolol-based antifungal agents in crop protection.
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http://dx.doi.org/10.1021/acs.jafc.1c01838DOI Listing
October 2021

Comparative Mitogenomics and Phylogenetic Analyses of Pentatomoidea (Hemiptera: Heteroptera).

Genes (Basel) 2021 Aug 25;12(9). Epub 2021 Aug 25.

Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Pentatomoidea is the largest superfamily of Pentatomomorpha; however, the phylogenetic relationships among pentatomoid families have been debated for a long time. In the present study, we gathered the mitogenomes of 55 species from eight common families (Acanthosomatidae, Cydnidae, Dinidoridae, Scutelleridae, Tessaratomidae, Plataspidae, Urostylididae and Pentatomidae), including 20 newly sequenced mitogenomes, and conducted comparative mitogenomic studies with an emphasis on the structures of non-coding regions. Heterogeneity in the base composition, and contrasting evolutionary rates were encountered among the mitogenomes in Pentatomoidea, especially in Urostylididae, which may lead to unstable phylogenetic topologies. When the family Urostylididae is excluded in taxa sampling or the third codon positions of protein coding genes are removed, phylogenetic analyses under site-homogenous models could provide more stable tree topologies. However, the relationships between families remained the same in all PhyloBayes analyses under the site-heterogeneous mixture model CAT + GTR with different datasets and were recovered as (Cydnidae + (((Tessaratomidae + Dinidoridae) + (Plataspidae + Scutelleridae)) + ((Acanthosomatidae + Urostylididae) + Pentatomidae)))). Our study showed that data optimizing strategies after heterogeneity assessments based on denser sampling and the use of site-heterogeneous mixture models are essential for further analysis of the phylogenetic relationships of Pentatomoidea.
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http://dx.doi.org/10.3390/genes12091306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471585PMC
August 2021

Anammox Bacteria Are Potentially Involved in Anaerobic Ammonium Oxidation Coupled to Iron(III) Reduction in the Wastewater Treatment System.

Front Microbiol 2021 10;12:717249. Epub 2021 Sep 10.

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences (CAS), Xiamen, China.

Anaerobic ammonium oxidation coupled to nitrite reduction (termed as Anammox) was demonstrated as an efficient pathway to remove nitrogen from a wastewater treatment system. Recently, anaerobic ammonium oxidation was also identified to be linked to iron(III) reduction (termed Feammox) with dinitrogen, nitrite, or nitrate as end-product, reporting to enhance nitrogen removal from the wastewater treatment system. However, little is known about the role of Anammox bacteria in the Feammox process. Here, slurry from wastewater reactor amended with ferrihydrite was employed to investigate activity of Anammox bacteria in the Feammox process using the N isotopic tracing technique combined with 16S rRNA gene amplicon sequencing. A significantly positive relationship between rates of N production and iron(III) reduction indicated the occurrence of Feammox during incubation. Relative abundances of Anammox bacteria including , , , and unclassified Brocadiaceae were detected with low relative abundances, whereas Geobacteraceae dominated in the treatment throughout the incubation. N production rates significantly positively correlated with relative abundances of , unclassified Geobacteraceae, and Anammox bacteria, revealing their contribution to nitrogen generation Feammox. Overall, these findings suggested Anammox bacteria or cooperation between Anammox bacteria and iron(III) reducers serves a potential role in Feammox process.
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http://dx.doi.org/10.3389/fmicb.2021.717249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461334PMC
September 2021

Prediction of Mental Health in Medical Workers During COVID-19 Based on Machine Learning.

Front Public Health 2021 7;9:697850. Epub 2021 Sep 7.

Northeast Asian Research Center, Jilin University, Changchun, China.

Mental health prediction is one of the most essential parts of reducing the probability of serious mental illness. Meanwhile, mental health prediction can provide a theoretical basis for public health department to work out psychological intervention plans for medical workers. The purpose of this paper is to predict mental health of medical workers based on machine learning by 32 factors. We collected the 32 factors of 5,108 Chinese medical workers through questionnaire survey, and the results of Self-reporting Inventory was applied to characterize mental health. In this study, we propose a novel prediction model based on optimization algorithm and neural network, which can select and rank the most important factors that affect mental health of medical workers. Besides, we use stepwise logistic regression, binary bat algorithm, hybrid improved dragonfly algorithm and the proposed prediction model to predict mental health of medical workers. The results show that the prediction accuracy of the proposed model is 92.55%, which is better than the existing algorithms. This method can be used to predict mental health of global medical worker. In addition, the method proposed in this paper can also play a role in the appropriate work plan for medical worker.
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http://dx.doi.org/10.3389/fpubh.2021.697850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452905PMC
September 2021
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