Department of BiologyPayame Noor University TehranTehranIran
Tehran, Tehran | Iran (Islamic Republic of)
Main Specialties: Biology, Biotechnology, Molecular Genetic Pathology, Rheumatology
Additional Specialties: Signal transduction
Primary Affiliation: Department of BiologyPayame Noor University TehranTehranIran - Tehran, Tehran , Iran (Islamic Republic of)
4PubMed Central Citations
J Cell Mol Med 2018 12 15;22(12):6401-6404. Epub 2018 Oct 15.
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
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Clin Rheumatol 2018 Sep 16;37(9):2471-2478. Epub 2018 Apr 16.
Rheumatology Research Center, Firoozgar Hospital, Iran University of Medical Science, Tehran, Iran.
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Journal of Medicinal Plants
Investigation of Punicic Acid Effects on Matrix Metalloproteinase Genes Expression in Bovine Fibroblast like-Synoviocytes as a Model of Osteoarthritis Taherian (M.Sc.)1, 2, Maghsoudi H (Ph.D.)1*, Vaziri A (Ph.D.)3, Alebouyeh M (Ph.D.)4 1- Biotechnology Research Center, Payame Noor University, Tehran, Iran 2- Food and Drug Research Institute, Iran Food and Drug Organization (FDO), Ministry of Health and Medical Education (MOH), Tehran, Iran 3- Department of Biology, Payame Noor University, Tehran, Iran 4- Center of Food and Drug Control References Laboratories (CFDCRL), Food and Drug Organization (FDO), Ministry of Health and Medical Education (MOH), Tehran, Iran * Corresponding author: Biotechnology Research Center, Payame Noor University Tehran, Iran Tel: +98 – 9198318489, Fax: 021-33416831 E-mail: email@example.com Received: 23 Jan. 2018 Accepted: 28 May 2018 Abstract Background: Osteoarthritis (OA) is a progressive, age-associated disease that is characterized with cartilage destruction, subchondral bone remodeling and inflammation of the synovial membrane. Considering the complications of common treatments of OA, including non-steroidal anti-inflammatory drug (NSAIDs) and corticosteroids, investigating new treatments for this disorder is crucial. Recently, the role of matrix metalloproteinases (MMPs) expression in pathogenesis of OA has attracted attention. Objective: This study aimed to explore the effect of punicic acid (PA) in inhibition of MMPs gene expression in LPS-stimulated Bovine Fibroblast-like synoviocytes (BFLS) as a model of OA. 30 Methods: In the first stage, the toxicity of PA was measured using MTT assay on BFLS cells. Afterward, the cells were stimulated by LPS (Lipopolysaccharide) and MMPs (Matrix Metalloproteinase) expression level in the BFLS cells were investigated using Real-Time PCR, in vitro Migration and Gelatin Zymography, Western Blot Analysis, ELISA Assay and Invasion Assay. Results: The results showed that PA significantly decreased MMP-9 expression levels in LPS-stimulated BFLS cells; also, it suppressed migration and invasion of the mentioned cells. However, PA had no significant effect on MMP-1-2-3. 30 Conclusion: Based on our results PA could significantly reduce the activity and inflammatory effect of MMP-9 in OA, its potential role as a supplementary agent to common NSAIDs and corticosteroids was confirmed. Nonetheless, cellular modeling does not significantly confirm the beneficial effect of OA in patients. 31 Keywords: Bovine Fibroblast-like synoviocyte, Lipopolysaccharide, Metalloproteinase, Osteoarthritis, Punicic acid 1 1 1 Introduction
Journal of medicinal plants
URL: http://jmp.ir/article-1-2179-en.htmlBackground: Osteoarthritis (OA) is one of the main causes of physical disability worldwide. Considering the complications of common treatments of OA, including non-steroidal anti-inflammatory drug (NSAIDs) and corticosteroids, establishment of new treatments is crucial.Objective: This study aimed to explore the effect of Echinops cephalotes extract on the main inflammatory biomarkers in OA.Methods: Hydroalcoholic extract of Echinops cephalotes, Ibuprofen and betamethasone were prepared to investigate their effects on inflammatory biomarkers. Human monocyte/macrophage (THP-1) cells and chondrocytes cells were used as a model of monocyte/macrophage and human cartilage cells in osteoarthritis. Lipopolysaccharide (LPS) was used to induce production of inflammatory cytokines in both cells. After RNA extraction and production of cDNA, RT_PCR & PCR were done. Then Real Time-PCR was used to investigate the amount of expression of proinflammatory genes.Results: Echinops cephalotes extract reduced mRNA expression level of proinflammatory cytokines in the cells induced by LPS. Moreover, production of PGE2 and NO in in the LPS-induced THP-1 cells was reduced by this extract. Ibuprofen and betamethasone were more effective in reducing above inflammatory agents than the extract.Conclusion: Echinops cephalotes extract can be used as a supplementary treatment option in osteoarthritis to reduce NSAIDs and corticosteroids dose in treatment of this disease.
Health Biotechnology and Biopharma
Due to the side effects of current therapies for osteoarthritis one of the alternative medicine is using herbal medicine such as Nigella sativa L. We examined that alcoholic extract of Nigella sativa (AENS) has an anti-inflammatory activity. Cells were activated with 100 ng/ml lipopolysaccharide for 24 h and cell supernatants were analyzed for PGE2 and nitrite content. One set of cells was activated for 1 h with LPS (100 ng/ml) for both reverse-transcriptase PCR and real-time PCR analysis of TNF-a, IL-1b , COX-2, and iNOS expression. AENS also reduced TNF-α and IL-1b expression in LPS-activated THP-1 cells. The present study demonstrates that the anti-inflammatory activity of AENS is not restricted to synoviocytes, but also affects monocyte macrophage-like cells that serve as a prototype for macrophages in the synovial membrane. These observations provide a scientific rationale for the pain-reducing and antiinflammatory effects of AENS observed in osteoarthritis patients. Keywords: Nigella sativa .L, bovine fibroblast-like, osteoarthritis, proinflammatory cytokine
Tehran Univ Med J
Background: Most of colorectal cancers (CRC) have originated from intestinal polyps. Evaluating of the expression level of genes that are involved in tumors growth and development, may consider as diagnostic factor of malignancy in the polyps. AXIN2 regulates the level of nuclear β-catenin in a negative-feedback loop there by being a negative regulator and target gene at the same time. The aims of current study were to examine the expression level of the AXIN2 in the colonic polyps and its linkage with the pathological features of the polyps.Methods: In the present analytical-descriptive study, the investigated population was chosen from the cases with colonic polyps that referred to the Gastroenterology and Liver Diseases Research Center, Taleghani Hospital, Tehran, Iran, from October 2014 to April 2015. Forty four biopsy polyp samples and 10 normal tissue samples were collected, as well as the demographic and clinical properties of the patients and the expression level of AXIN2 gene was quantified by Real-time PCR. The outcomes were analyzed by the ABI Prism 7500 Sequence Detection System (SDS) software, version 2.1.0 (Applied Biosystems Inc., Foster City, CA, USA) and GraphPad Prism, version 3 (GraphPad Software Inc., La Jolla, CA, USA) Also, the expression changes of the intended gene in target groups were compared with the normal tissues using the 2-ΔΔCtequation.Results: The data showed enhanced level of the expression of AXIN2 gene in the colonic polyps in comparison to the normal tissues (RQ>2), which was significantly upper in adenoma polyps compared to the hyperplastic group (P=0.015). Also, unlike the rectum, the AXIN2 gene activity in colon area was higher than normal tissue.Conclusion: The results of the current study show that the expression pattern of AXIN2 gene, was markedly changed during the transformation of the normal tissue to polyp. The increased expression level of this gene could be applied as a diagnostic marker in dissociation of the adenoma polyps from hyperplastic ones. On the other hand, the location of the polyps modulates the AXIN2 gene function. Taking together, evaluating the changes of AXIN2, has a precise diagnostic value in the CRC related studies.
Objective: Fetal hemoglobin is the predominant hemoglobin expressed by gamma globin. However, in adults, fetal hemoglobin normally reduces to very low levels of the total hemoglobin. The increase in levels of fetal hemoglobin can ameliorate the severity of βhemoglobin disorders such as sickle cell disease and beta-thalassemia. Currently, drugs that have been used for induction of Fetal hemoglobin (HbF) have short-term effects. Dendrosomal nano-curcumin (DNC), which has high solubility and absorption, is able to detect different targets in the cell and affect gene expression. LSD1 is one of the most important gamma globin inhibitors. In this study, we examine the capability of DNC to inhibit the expression of LSD1, GATA1, and FOG1 as well as the increase in gamma globin expression. Methods: We used the K562 cell line for the MTT assay and treatment by DNC. Then the effect of DNC on the increase in expression level of γ-globin, decrease expression level of LSD1 and transcription factors, GATA1 and FOG1was investigated by Real time PCR. Results: Data acquired from gene expression assays indicated that DNC induced gamma globin expression and decreased expressions of LSD1, GATA1, and FOG1 in a time and dose-dependent manner. Conclusion: Inhibition of LSD1, GATA1, and FOG1 expressions via DNC led to increased gamma globin expression. These results showed that DNC could be a promising treatment for beta-thalassemia and sickle cell disorders, and possibly reduce the severity of symptoms of these patients through the induction of fetal hemoglobin. Keywords: Dendrosomal nano-curcumin, γ-globin, LSD1, K562 cell line
Gastrointest Tumors 2016 Sep 17;3(1):44-58. Epub 2016 Mar 17.
Department of Biotechnology, Faculty of Science, Payame Noor University, Tehran.
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Arak Medical University Journal (AMUJ)
Abstract Background: Streptokinase is one of the most common and cost effective fibrinolytic drugs for treatment of heart attacks and vein thrombosis. Unlike many advantages over other thrombolytic drugs, administration of streptokinase can produce some complications such as immunologic reactions, hemorrhage and incomplete treatment due to relative short half life. Pegylation is one of the most common methods for improving of these shortcomings. Materials and Methods: In this study, designing a proper candidate for specific pegylation with cysteine was done by means of SPDBviewer software. After a meaning ful mutation by SOEing PCR method, mutated (sk45cys) and intact SK (ski) genes were cloned in pET26-b vector and the structures were transformed in E.coli. Clones, Afrer growing, were expressed by IpTG and exptression of proteins was confirmed by SDS-PAGE and western blotting. The proteins were purified by affinity chromatography with NiNTA columns and amidolytic activity of purified proteins was assayed using chromogenic method and different concentrations of S2251 substrate. Results: Results of activity assays showed that amidolytic activity of SK45cys had about 10% increase in comparison to Ski, after 30 minutes of complex formation with plasminogen. Conclusion: Generally, it was concluded that, considering cys45 as a superficial aminoacid and also relative increase of activity, SK45cys can be considered a suitable protein for specific pegylation
International Journal of Contemporary Research and Review
www.ijcrr.inIn this study, we expressed and purified the recombinant baculovirus 373 K/E p53 protein in a baculovirus expression system to characterize this mutant and compare it with wild type p53. Gel- filtration chromatography and chemical cross-linking experiments indicated that purified recombinant baculovirus 373 K/E p53 protein assembles into multimeric forms ranging from tetramers to polymers. Gel-mobility shift assays and protein-DNA cross-linking studies demonstrated that the recombinant protein binds, to a consensus DNA target as a dimer but that additional p53 mutant molecules may then associate with the preformed p53-dimer-DNA complexes to form a larger p53_DNA complexes. These observations suggest that the p53 mutant tetramers and polymers that forms the minimal p53 mutant complex in solution dissociated upon DNA binding to form p53 mutant dimmer DNA complexes. The DNA binding activity of this mutant was then investigated using electrophoretic mobility shift assays as well as supershift assay with anti-p53 antibodies. Binding of the anti-p53 antibody PAb421to the oligomerization promoting domain on p53 stimulated the sequential formation of both the p53_dimer DNA and largerp53-DNA complexes