Publications by authors named "Hongzhi Wang"

312 Publications

The Incidence, Risk Factors, and Patterns of Peripherally Inserted Central Catheter-Related Venous Thrombosis in Cancer Patients Followed Up by Ultrasound.

Cancer Manag Res 2021 1;13:4329-4340. Epub 2021 Jun 1.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), ICU, Peking University Cancer Hospital & Institute, Beijing, People's Republic of China.

Purpose: A peripherally inserted central catheter (PICC) is associated with venous thromboembolism (VTE) especially in patients suffering from cancer. We analyzed the incidence, risk factors, and patterns of PICC-related VTE in cancer patients.

Patients And Methods: Patients with cancer who underwent PICC placement were evaluated retrospectively. Routine, prospective ultrasound post-PICC placement was used for asymptomatic and symptomatic patients to identify VTE. Multivariable logistic regression models with odds ratios (ORs) were used to examine VTE risk factors.

Results: Of 2353 PICCs placed, 165 patients (7.01%) developed PICC-related VTE with a median thrombosis time of 12 days. After adjustment of multivariable analysis, patients with PICC-related VTE were more likely to have a ratio of PICC diameter:vein diameter >0.35 (adjusted OR, 1.689; 95% CI, 1.023-2.789) and high level of triglycerides (1.561; 1.096-2.223). The prevalence of A (adjusted OR, 1.680; 95% CI, 1.009-2.798), B (1.835; 1.137-2.961), and AB (3.275; 1.840-5.829) blood group was significantly higher than that of the O blood group in VTE patients. Venous recanalization was observed in 44.8% (74/165) patients after anticoagulation therapy, and more often in patients with combined deep VTE than in patients with isolated superficial VTE (OR, 17.942; 95% CI, 5.427-59.316). The recanalization time was 20±5 (range, 10-31) days.

Conclusion: The non-O blood group, larger ratio of PICC diameter:vein diameter, and high level of triglycerides were significantly associated with PICC-related VTE. Almost half of cases of PICC-related deep VTE could be reversed by anticoagulation treatment.
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http://dx.doi.org/10.2147/CMAR.S301458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179741PMC
June 2021

Core-shell structured [email protected]@SiO filler for radiopacity and ultra-low shrinkage dental composite resins.

J Mech Behav Biomed Mater 2021 May 28;121:104593. Epub 2021 May 28.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, PR China.

To overcome the interfacial problem between X-ray radiopaque ZrO fillers and polymer resin in dental composites, monodispersed [email protected]@SiO (SZS) microspheres with narrow size distribution were prepared by a controlled sol-gel method. In the presence of SiO coating layer over [email protected] (SZ) microspheres, they were easily silanized same as SiO microspheres. Ethoxylated bisphenol A dimethacrylate (EBPADMA) with a higher molecular weight and a lower viscosity was used as base resin monomer mixed with a low amount of diluent triethylene glycol dimethacrylate (TEGDMA). Additionally, the addition of a small amount of pore agent acetone dicarboxylic acid (ADCA) produced some voids, thereby effectively reducing the polymerization shrinkage of the resin. The prepared dental composites combining 52 wt% monodispersed silica microsphere, 20 wt% SZS microspheres, exhibited significantly enhanced capacity in radiopacity (higher than tooth enamel) and very low shrinkage (<0.1%). It also has better mechanical properties than resin composites filled with SiO microspheres, and its strength can meet practical applications. The properties of the radiopaque dental composite were to be further tuned by varying the amount of SZS microspheres contents, and the radiopaque resin has an advantage over the commercial one in that it is clinically nondestructive.
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http://dx.doi.org/10.1016/j.jmbbm.2021.104593DOI Listing
May 2021

Positively charged collective oscillations induce efficient Aβ1-42 fibril degradation in the presence of novel [email protected] core/shell nanorods.

Chem Commun (Camb) 2021 Jun 3. Epub 2021 Jun 3.

Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications, Experimental Center of Advanced Materials, School of Materials Science and Engineering, Beijing Institute of Technology, China. and Key Laboratory of Medical Molecular Science and Pharmaceutical Engineering, MIIT, Institute for Engineering Medicine, BIT, Beijing 100081, China.

We synthesized [email protected] core/shell nanorods (NRs) that have synergistic surface plasmon resonance (SPR) effects using a new cation exchange process in ethylene glycol (EG) phase. The dual effect - NIR photothermal and surface positive charge plasmon - of [email protected] NRs, promote the capability to degrade Aβ1-42 fibrils into amorphous protein.
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http://dx.doi.org/10.1039/d1cc01470fDOI Listing
June 2021

Brain natriuretic peptide and cardiac troponin I for prediction of the prognosis in cancer patients with sepsis.

BMC Anesthesiol 2021 May 24;21(1):159. Epub 2021 May 24.

Department of Hepato-Pancreato-Biliary Surgery, Peking University Cancer Hospital & Institute, Beijing, People's Republic of China.

Background: This article aimed to study the value of brain natriuretic peptide (BNP) and cardiac troponin I(cTnI) for predicting the prognosis in cancer patients with sepsis.

Methods: A cohort of 233 cancer patients with sepsis admitted to our ICU from January 2017 to October 2020 was included in this retrospective study. The data of BNP and cTnI on the first day (d1) and the third day(d3) after entering ICU, blood lactate (Lac), procalcitonin (PCT), Leucocyte and Sequential Organ failure assessment (SOFA) scores within 24 hr of entering ICU, the incidence of septic shock, acute kidney injury(AKI), acute respiratory failure (ARF) or sepsis-induced myocardial dysfunction(SIMD) in ICU, fluid balance in 24 hr and 72 hr after entering ICU, time of mechanical ventilation(MV), length of stay, emergency surgery were collected. According to 28-day mortality, patients were divided into survival group (190 cases) and death group (43 cases). All the above variables were compared.

Results: BNP was an independent predictor for the mortality in these patients (P < 0.05).While cTnI was not. BNP on d3 in 681.5 pg/ml predicted the mortality with a sensitivity of 91.5 % and a specificity of 88.7 %. All patients were divided into the new two groups following the cutoff value of BNP on d3(681.5pg/ml), and the survival curve showed a significant difference with Kaplan-Meier analysis (P < 0.05). BNP had statistical differences between four groups based on the comorbidities(septic shock, AKI, ARF or SIMD), but cTnI was not.

Conclusions: BNP was a great predictor for the prognosis of cancer patients with sepsis, while cTnI was not.
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http://dx.doi.org/10.1186/s12871-021-01384-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142482PMC
May 2021

Abrasion Resistant/Waterproof Stretchable Triboelectric Yarns Based on Fermat Spirals.

Adv Mater 2021 May 24:e2100782. Epub 2021 May 24.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, P. R. China.

Emerging energy harvesting yarns, via triboelectric effects, have wide application prospects in new-generation wearable electronics. However, few studies have been carried out regarding simultaneously achieving high electrical performance, mechanical robustness, and comfortability in industrial-scalable yarn. Here, an electronic yarn twisted into Fermat spiral, which has outstanding dynamic structure stability, is reported. The Fermat-spiral-based energy yarns (FSBEY) can simultaneously realize ultrahigh abrasion resistance (over 5000 Martindale standard abrasion cycles), stable reversible strain (100%), and excellent electrical output. Considerably high output (105 V, ≈1.2 µA under 2 Hz) can be attained upon contacting a single yarn (30 cm) with latex material, which is superior to most state-of-the-art stretchable triboelectric yarns. The application of these FSBEY in wireless gesture recognition, smart screen information protection, and harvesting of energy from water dropletsis demonstrated. Moreover, textiles knitted from the FSBEY have distinguished waterproof nature and are breathable. This work shows a feasible proposal for building future "energy garments".
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http://dx.doi.org/10.1002/adma.202100782DOI Listing
May 2021

Non-Small-Cell Lung Cancer Regression by siRNA Delivered Through Exosomes That Display EGFR RNA Aptamer.

Nucleic Acid Ther 2021 May 17. Epub 2021 May 17.

Center for RNA Nanobiotechnology and Nanomedicine, College of Pharmacy, James Comprehensive Cancer Center, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio, USA.

Lung cancer is the second most common cancer in both men and women and is the leading cause of cancer death in the United States. The development of drug resistance to commonly used chemotherapeutics in non-small-cell lung cancer (NSCLC) poses significant health risks and there is a dire need to improve patient outcomes. In this study, we report the use of RNA nanotechnology to display ligand on exosome that was loaded with small interfering RNA (siRNA) for NSCLC regression in animal trials. Cholesterol was used to anchor the ligand targeting epidermal growth factor receptor on exosomes that were loaded with siRNA to silence the antiapoptotic factor survivin. The cytosolic delivery of siRNA overcame the problem of endosome trapping, leading to potent gene knockdown, chemotherapy sensitization, and tumor regression, thus achieving a favorable IC of 20 nmol/kg siRNA encapsulated by exosome particles in the gene knockdown assessment.
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http://dx.doi.org/10.1089/nat.2021.0002DOI Listing
May 2021

Liquid biopsies to distinguish malignant from benign pulmonary nodules.

Thorac Cancer 2021 Jun 7;12(11):1647-1655. Epub 2021 May 7.

Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital, Anhui Medical University, Hefei, China.

Over the past decades, low-dose computed tomography (LD-CT) screening has been widely used for the early detection of lung cancer. Increasing numbers of indeterminate pulmonary nodules are now being discovered. However, it remains challenging to distinguish malignant from benign pulmonary nodules, especially those considered to be small or ground-glass (GGN) nodules. Liquid biopsies have been successfully applied in the diagnosis of advanced lung cancer, and the potential value for early detection of lung cancer has made great progress. Recent studies have demonstrated the value of various blood-based tumor biomarkers in determining the nature of pulmonary nodules, including cell-free DNA (cfDNA), microRNAs (miRNAs), circulating tumor cells (CTCs) and tumor-associated autoantibodies (AAbs). In this review, we summarize the latest progress of liquid biopsies, and their potential applications and challenges in the diagnosis of malignant pulmonary nodules.
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http://dx.doi.org/10.1111/1759-7714.13982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169297PMC
June 2021

Hybrid Plasmonic Nanodumbbells Engineering for Multi-Intensified Second Near-Infrared Light Induced Photodynamic Therapy.

ACS Nano 2021 05 7;15(5):8694-8705. Epub 2021 May 7.

Beijing Key Laboratory of Construction-Tailorable Advanced Functional Materials and Green Applications, School of Materials Science & Engineering, Institute of Engineering Medicine, Beijing Institute of Technology, Beijing 100081, China.

Photodynamic therapy (PDT) has shown great potential in infection treatment. However, the shallow depth of the short wavelength light and the low reactive oxygen species (ROS) production hinder its development. A strategy that can achieve a second near-infrared (NIR-II) light that is a long wavelength induced multi-intensified antibacterial PDT is most critical. Herein, hybrid plasmonic Au/CdSeS with precise Ag doping (ACA) nanodumbbells are rationally designed for ideal NIR-II light induced antibacterial PDT. Plasmonic Au nanorods extend the photocatalytic activity of ACA to NIR-II regions, which provides a basis for NIR-II light induced PDT. More importantly, multi-intensified PDT can be realized by the following creativities: (i) elaborate design of as-synthesized nanodumbbells that allows for electron holes to be redistributed in different regions simultaneously, (ii) the efficient hot-electrons injection that benefits from the ratio tailoring of anions ratio of Se and S, and (iii) the dopant Ag level inhibiting the combination of electron holes. The nanodumbbells create effective hot-electrons injection and a separation of electron holes, which provides great convenience for the production of ROS and allows NIR-II light induced PDT for the inhibition of bacteria and biofilms. As a result, comparably, our well-defined ACA hybrid nanodumbbells can generate about 40-fold superoxide radicals (·O) and more hydroxyl radicals (·OH). Therefore, the MIC value of the as-synthesized nanodumbbells is lower than the value of 1/16 of core-shell ACA. results further demonstrate that our nanodumbbells exhibit excellent PDT efficacy.
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http://dx.doi.org/10.1021/acsnano.1c00772DOI Listing
May 2021

Efficient Framework Analysis for Targeted Drug Delivery Based on Internet of Bio-NanoThings.

Arab J Sci Eng 2021 Apr 22:1-16. Epub 2021 Apr 22.

Department of Electronics and Electrical Communications Engineering, Menoufia University, Menouf, 32952 Egypt.

The Internet of Bio-NanoThings (IoBNTs) is a novel paradigm that derives from synthetic biology and advances in nanotechnology for controlling the embedded nanodevices in various medical applications. However, numerous studies have focused on communication efficiency among the nanodevices in a given network, the challenges such as the design and the development of the nanodevices, and the coordination of molecular communication within the wireless body area network (BAN), and the interface connection between the BAN and the Internet are yet to be addressed. Therefore, in this study, we present a framework analysis comprising of the compartmental model, for studying the effects and variances in drug concentration that occur inside intra-body nanonetworks through IoBNT, while taking into account the properties of target cells as well as the ligand-receptor binding mechanism. A performance analysis of the proposed framework for the forward link (i.e., from the Internet to the intra-body nanonetwork) and reverse link (i.e., from the intra-body nanonetwork to the Internet) is presented. The simulation results of the developed framework reveal its ability to enhance the delivery of therapeutic drugs to the target cell while minimizing the side effects in healthy cells.
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http://dx.doi.org/10.1007/s13369-021-05651-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061466PMC
April 2021

Continuous preparation of dual-responsive sensing fibers for smart textiles.

J Colloid Interface Sci 2021 Sep 9;597:215-222. Epub 2021 Apr 9.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.

Continuous preparation of sensing fibers that respond to multiple stimuli is of great significance to the development of smart textile and clothing. However, in most cases, the production of sensing fibers is restricted to laboratory scale by factors of equipment and technology, thus it is still challenging to achieve industrial-scale fibers. In this study, continuous preparation of dual-responsive sensing fibers (DRSF) by a set of process designs with custom-built equipment, which consists of core fibers, functional layers, parallel electrodes and protective layers are reported. Combining vanadium pentoxide (VO) nanobelts with a unique device design, DRSFs exhibited a significant electrical output when stimulated by heat or blue light (460 nm), where the factors of the aspect ratio of one-dimensional nanostructures have also been explored. Furthermore, proof-of-concept electronic textiles with DRSFs woven into fabric were demonstrated.
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http://dx.doi.org/10.1016/j.jcis.2021.04.015DOI Listing
September 2021

K-SEIR-Sim: A simple customized software for simulating the spread of infectious diseases.

Comput Struct Biotechnol J 2021 7;19:1966-1975. Epub 2021 Apr 7.

Department of Endocrinology, Shanghai Gongli Hospital, The Second Military Medical University, Shanghai 200135, China.

Infectious disease is a great enemy of humankind. The ravages of COVID-19 are leading to profound crises across the world. There is an urgent requirement for analyzing the current pandemic situation, predicting trends over time, and assessing the effectiveness of containment measures. Thus, numerous statistical models, primarily based on the susceptible-exposed-infected-recovered or removed (SEIR) model, have been established. However, these models are highly technical, which are difficult for the public and governing bodies to understand and use. To address this issue, we developed a simple operating software based on our improved K-SEIR model termed as the kernelkernel SEIR simulator (K-SEIR-Sim). This software includes natural propagation parameters, containment measure parameters, and certain characteristic parameters that can deduce the effects of natural propagation and containment measures. Further, the applicability of the proposed software was demonstrated using the example of the COVID-19 outbreak in the United States and the city of Wuhan, China. Operating results verified the potency of the proposed software in evaluating the epidemic situation and human intervention during COVID-19. Importantly, the software can perform real-time, backward-looking, and forward-looking analysis by functioning in data-driven and model-driven ways. All of them have considerable practical values in their applications according to the actual needs of personal use. Conclusively, K-SEIR-Sim is the first simple customized operating software that is highly valuable for the global fight against COVID-19 and other infectious diseases.
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http://dx.doi.org/10.1016/j.csbj.2021.04.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025586PMC
April 2021

Correlation between early brain natriuretic peptide level and mortality in cancer patients with septic shock.

Ann Palliat Med 2021 Apr 23;10(4):4214-4219. Epub 2021 Mar 23.

Department of Hepato-Pancreato-Biliary Surgery, Peking University Cancer Hospital & Institute, Beijing, China.

Background: This study aimed to examine the correlation between early brain natriuretic peptide (BNP) levels and mortality in cancer patients with septic shock.

Methods: A retrospective analysis of 159 cancer patients with septic shock admitted to the intensive care unit (ICU) from Dec. 2012 to Dec. 2019 was performed. BNP levels and other variables, including blood lactate (Lac), procalcitonin (PCT), white blood cell (WBC) counts, acute physiology and chronic health status system II scores (APACHE-II scores) were collected within 24 hours after ICU admission. According to 28-day mortality, patients were divided into a death group (60 cases) and a survival group (99 cases). All variables were compared by univariate analysis, and then a multiple logistic regression analysis was performed on the variables that showed significant differences. Receiver operating characteristic curve (ROC curve) analysis was used to evaluate the predictive value of BNP on mortality in cancer patients with septic shock.

Results: BNP, APACHE-II score, Lac, and PCT in the death group were significantly higher than those in the survival group (P<0.05). Multiple logistic regression analysis of these four variables indicated that BNP, APACHE-II score and Lac were independent risk predictors of mortality in these patients (P<0.05). The BNP level at 899.6 pg/mL predicted mortality with a sensitivity of 76.7% and a specificity of 84.7%. The area under the ROC curve was 0.86±0.03 (P<0.05) for BNP, which was significantly larger than that of the APACHE-II score (P<0.05) and Lac (P<0.05).

Conclusions: BNP was an independent risk factor for mortality in cancer patients with septic shock, and had a higher predictive value than the APACHE-II score and Lac.
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http://dx.doi.org/10.21037/apm-20-2212DOI Listing
April 2021

The changes in bone turnover markers of female systemic lupus erythematousus patients without glucocorticoid.

Lupus 2021 May 9;30(6):965-971. Epub 2021 Mar 9.

Department of Rheumatology and Immunology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

Objectives: SLE is a chronic autoimmune disease, which can affect the level of bone metabolism and increase the risk of osteoporosis and fracture. The purpose of this research is to study the effect of SLE on bone turnover markers without the influence of glucocorticoids.

Methods: A total of 865 female subjects were recruited from Zhejiang Provincial People's Hospital and the First Hospital of Jiaxing, including 391 SLE patients without the influence of glucocorticoids and 474 non-SLE people. We detected Bone turnover markers including amino-terminal propeptide of type 1 procollagen (P1NP), C-terminal turnover of β - I collagen (β-CTX), N-terminal midfragment of osteocalcin (NMID) and 25(OH)D, and analyzed the difference in Bone turnover markers between the SLE group and the control group, as well as the influence of age and season on bone metabolism in female SLE patients.

Results: In the SLE group, the average age was 43.93±13.95 years old. In the control group, the average age was 44.84±11.42 years old. There was no difference between the two groups (t = 1.03, P = 0.30). P1NP, NMID and 25(OH)D in the SLE group were significantly lower than those in the control group (Z = 8.44, p < 0.001; Z = 14.41, p < 0.001; Z = 2.19, p = 0.029), and β-CTX in the SLE group was significantly higher than that in the control group (Z = 2.61, p = 0.009). In addition, the levers of β-CTX, NMID, P1NP and 25(OH)D in older SLE female patients were statistically significantly higher than those in younger (ρ = 0.104, p = 0.041; ρ = 0.223, p < 0.001; ρ = 0.105, p = 0.038; ρ = 0.289, p < 0.001). Moreover, β-CTX reached a high value in summer and PINP reached a low value in winter.

Conclusion: The bone formation markers of female SLE patients without glucocorticoid were lower than those of normal people and the bone resorption marker was higher than that of normal people. The 25 (OH) D of female SLE patients without glucocorticoid was lower than that of normal people. The risk of osteoporosis and fracture may be higher in elderly women with SLE. The bone resorption level of female SLE patients is high in summer and the bone formation level is low in winter.
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http://dx.doi.org/10.1177/09612033211000126DOI Listing
May 2021

Flexible and high-performance electrochromic devices enabled by self-assembled 2D TiO/MXene heterostructures.

Nat Commun 2021 Mar 11;12(1):1587. Epub 2021 Mar 11.

Department of Chemistry, Boston University, Boston, MA, USA.

Transition metal oxides (TMOs) are promising electrochromic (EC) materials for applications such as smart windows and displays, yet the challenge still exists to achieve good flexibility, high coloration efficiency and fast response simultaneously. MXenes (e.g. TiCT) and their derived TMOs (e.g. 2D TiO) are good candidates for high-performance and flexible EC devices because of their 2D nature and the possibility of assembling them into loosely networked structures. Here we demonstrate flexible, fast, and high-coloration-efficiency EC devices based on self-assembled 2D TiO/TiCT heterostructures, with the TiCT layer as the transparent electrode, and the 2D TiO layer as the EC layer. Benefiting from the well-balanced porosity and connectivity of these assembled nanometer-thick heterostructures, they present fast and efficient ion and electron transport, as well as superior mechanical and electrochemical stability. We further demonstrate large-area flexible devices which could potentially be integrated onto curved and flexible surfaces for future ubiquitous electronics.
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http://dx.doi.org/10.1038/s41467-021-21852-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952574PMC
March 2021

Simultaneous Integrated Boost Intensity-Modulated Radiation Therapy Can Benefit the Locally Advanced Rectal Cancer Patients With Clinically Positive Lateral Pelvic Lymph Node.

Front Oncol 2020 22;10:627572. Epub 2021 Feb 22.

Department of Radiation Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, Beijing, China.

Background And Purpose: The optimal treatment modality for clinically positive lateral pelvic lymph node (LPLN) from locally advanced rectal cancer (LARC) is unknown. Thus, we aimed to analyze the optimal radiotherapy dose for clinically positive LPLN from LARC.

Materials And Methods: We retrospectively evaluated distal LARC (i.e., within 8 cm from the anal verge) patients with clinically positive LPLN (i.e., ≥7 mm in the short axis). They were divided into two groups based on whether or not they received simultaneous integrated boost intensity-modulated radiation therapy (SIB-IMRT)-based chemoradiotherapy. The total radiotherapy dose on LPLN were 56-60Gy for SIB-IMRT group and 41.8Gy for non-SIB-IMRT group. The clinical parameters and regrowth rate of LPLN were then compared between the two groups.

Results: A total of 151 patients were evaluated, and 83 and 68 patients were classified to the SIB-IMRT and non-SIB-IMRT group, respectively. The median follow-up period was 22.6 months, and the 2-year LPLN regrowth rate was significantly different between the SIB-IMRT group and the non-SIB-IMRT group (0% vs 10.8%, P=0.024). Further, SIB-IMRT yielded a significantly lower 2-year LPLN regrowth rate in patients whose LPLN measured ≥8 mm in the short axis (0% vs. 15.9%, P=0.019) or ≥10 mm in the long axis (0% vs. 17.6%, P=0.024) compared to patients who were in non-SIB-IMRT group. Meanwhile, there was no significant difference in grade II radiation-related toxicity (30.1% vs. 39.1%, P=0.217) and surgical complications (21.8% vs. 12.2%, P=0.198) between the two groups.

Conclusion: SIB-IMRT-based neoadjuvant chemoradiotherapy is beneficial for eliminating clinically positive LPLN from LARC without increasing the incidence of radiotherapy-related toxicity and surgical complications, and patients with larger LPLN may gain benefit from this technique.
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http://dx.doi.org/10.3389/fonc.2020.627572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937798PMC
February 2021

Identification of FPR3 as a Unique Biomarker for Targeted Therapy in the Immune Microenvironment of Breast Cancer.

Front Pharmacol 2020 11;11:593247. Epub 2021 Feb 11.

Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.

Although research into immunotherapy is growing, its use in the treatment of breast cancer remains limited. Thus, identification and evaluation of prognostic biomarkers of tissue microenvironments will reveal new immune-based therapeutic strategies for breast cancer. Using an in silico bioinformatic approach, we investigated the tumor microenvironmental and genetic factors related to breast cancer. We calculated the Immune score, Stromal score, Estimate score, Tumor purity, TMB (Tumor mutation burden), and MATH (Mutant-allele tumor heterogeneity) of Breast cancer patients from the Cancer Genome Atlas (TCGA) using the ESTIMATE algorithm and Maftools. Significant correlations between Immune/Stromal scores with breast cancer subtypes and tumor stages were established. Importantly, we found that the Immune score, but not the Stromal score, was significantly related to the patient's prognosis. Weighted correlation network analysis (WGCNA) identified a pattern of gene function associated with Immune score, and that almost all of these genes (388 genes) are significantly upregulated in the higher Immune score group. Protein-protein interaction (PPI) network analysis revealed the enrichment of immune checkpoint genes, predicting a good prognosis for breast cancer. Among all the upregulated genes, FPR3, a G protein-coupled receptor essential for neutrophil activation, is the sole factor that predicts poor prognosis. Gene set enrichment analysis analysis showed FRP3 upregulation synergizes with the activation of many pathways involved in carcinogenesis. In summary, this study identified FPR3 as a key immune-related biomarker predicting a poor prognosis for breast cancer, revealing it as a promising intervention target for immunotherapy.
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http://dx.doi.org/10.3389/fphar.2020.593247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928373PMC
February 2021

Wicking-Polarization-Induced Water Cluster Size Effect on Triboelectric Evaporation Textiles.

Adv Mater 2021 Apr 3;33(15):e2007352. Epub 2021 Mar 3.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, P. R. China.

Sweating during exercise, physical labor, or hot weather leads to a feeling of discomfort. The stuffiness, stickiness, and heaviness brought by sweat may promote negative emotions or disease. Clothing, textiles, and wearable devices exacerbate these problems by restricting evaporation of sweat. Here, a textile that can promote and enhance sweat evaporation by coupling wicking and polarization is reported. The wicking is produced by the wettability gradient and pore size, which make the surface moisture content of the textile in contact with the skin strictly 0%. The polarization is driven by a ferroelectric-enhanced triboelectric textile. This textile degrades large-sized water clusters into small-sized water clusters or water monomers, so that the textiles have an excellent moisture evaporation rate (4.4 and 3.6 times faster than the cotton and polyester textiles, respectively). This work provides a new source of inspiration for quick-drying textiles and also finds an attractive application for triboelectric technology.
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http://dx.doi.org/10.1002/adma.202007352DOI Listing
April 2021

Arsenic Dispensing Powder Promotes Erythropoiesis in Myelodysplastic Syndromes via Downregulation of HIF1A and Upregulation of GATA Factors.

Am J Chin Med 2021 25;49(2):461-485. Epub 2021 Feb 25.

Divisions of Pathology and Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, OH, USA.

Traditional Chinese Medicine (TCM) is a practical medicine based on thousands of years of medical practice in China. Arsenic dispensing powder (ADP) has been used as a treatment for MDS patients with a superior efficacy on anemia at Xiyuan Hospital of China Academy of Chinese Medical Sciences. In this study, we retrospectively analyzed MDS patients that received ADP treatment in the past 9 years and confirmed that ADP improves patients' anemia and prolongs overall survival in intermediate-risk MDS patients. Then, we used the MDS transgenic mice model and cell line to explore the drug mechanism. In normal and MDS cells, ADP does not show cellular toxicity but promotes differentiation. In mouse MDS models, we observed that ADP showed significant efficacy on promoting erythropoiesis. In the BFU-E and CFU-E assays, ADP could promote erythropoiesis not only in normal clones but also in MDS clones. Mechanistically, we found that ADP could downregulate HIF1A in MDS clones through upregulation of VHL, P53 and MDM2, which is involved in two parallel pathways to downregulate HIF1A. We also confirmed that ADP upregulates GATA factors in normal clones. Thus, our clinical and experimental studies indicate that ADP is a promising drug to promote erythropoiesis in both MDS and normal clones with a superior outcome than current regular therapies. ADP promotes erythropoiesis in myelodysplastic syndromes via downregulation of HIF1A and upregulation of GATA factors.
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http://dx.doi.org/10.1142/S0192415X2150021XDOI Listing
February 2021

Stereotactic body radiotherapy prolongs the progression-free survival and delays the change of systemic therapy regimen in patients with lung oligoprogressive metastatic colorectal cancer.

Asia Pac J Clin Oncol 2021 Feb 25. Epub 2021 Feb 25.

Department of Radiation Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, Beijing, China.

Purpose: To analyze the effect of stereotactic body radiotherapy (SBRT) on colorectal cancer (CRC) patients with lung oligoprogression (OP).

Method: Patients with lung OP from CRC treated by SBRT at our center were included in this retrospective analysis. The progression-free survival (PFS), change of systemic therapy (CST), local control (LC), and overall survival (OS) were analyzed. Cumulative incidence was used to report CST, and the Kaplan-Meier method was used to evaluate PFS and LC.

Results: A total of 17 patients with 38 lung OP lesions treated by SBRT from October 2012 to December 2018 were involved. All patients had undergone radical resection for primary CRC and administered with standard systemic therapy regimens (seven for the first line and 10 for the second line). Among them, nine (52.9%) had received targeted therapy before SBRT, 14 (82.4%) patients underwent chemotherapy, and 12 received targeted therapy after SBRT. Six patients (35.3%) underwent CST after a median time of 5.2 months (range: 1.7-27.5 months). The median follow-up was 9.9 months, and the 1-year OS rate for all patients was 73.5%. Progression was observed in of 14 of 17 patients (82.4%), and the 6-month PFS for all patients was 25.9%. Univariate analysis indicated that only targeted therapy before SBRT was a beneficial prognostic indicator for 6-month PFS (P = .026) and N-PFS (P = .013). The 1-year LC for all 38 lesions was 77.8%, and during and after SBRT, no grade 3 or higher toxicities were observed.

Conclusion: SBRT combined with systemic therapy made partial CRC patients with lung OP avoid the progress within 6 months and delayed the need for CST to 5.2 months, and targeted therapy before SBRT was a positive indicator of PFS.
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http://dx.doi.org/10.1111/ajco.13557DOI Listing
February 2021

Cartilage oligomeric matrix protein affects the biological behavior of papillary thyroid carcinoma cells by activating the PI3K/AKT/Bcl-2 pathway.

J Cancer 2021 15;12(6):1623-1633. Epub 2021 Jan 15.

Department of Endocrine and Metabolism, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China.

To explore the effect of cartilage oligomeric matrix protein (COMP) on papillary thyroid carcinoma (PTC). COMP expression levels in PTC tissues and matched adjacent normal tissues were measured using tissue microarrays. Human PTC cells were cultured and transduced with lentiviral short hairpin RNA against COMP (COMP-shRNA), a negative control (NC) shRNA, or mock transfected (Control). We used the Cell Counting Kit-8, performed colony formation assays, wound healing assays, Transwell invasion assays, flow cytometry, and measured the expression of apoptosis-related proteins at the mRNA and protein levels to explore the effects of COMP on the biological behavior of PTC cells and to discover the specific signaling pathway involved in these processes. COMP expression was significantly higher in PTC tissues than in adjacent normal tissues. At the cellular level, COMP promoted cell migration, increased the invasiveness of PTC cells, and inhibited apoptosis. However, differences in cell proliferation were only observed within 72 hours. At the same time, colony formation assays showed that silencing COMP inhibited the proliferation of PTC cells. We also found that COMP regulated the behavior of PTC cells by activating the PI3K/AKT/Bcl-2 pathway. COMP is upregulated in PTC, which enhances cancer cell invasion and inhibits apoptosis, contributing to the development and progression of PTC. Thus, COMP may serve as a new biomarker for PTC.
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http://dx.doi.org/10.7150/jca.49144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890313PMC
January 2021

Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway.

J Orthop Surg Res 2021 Feb 20;16(1):150. Epub 2021 Feb 20.

Department of Geriatrics, Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Nangang District, Harbin, 150086, China.

Objective: We aimed to investigate the therapeutic effects of Moringa oleifera leaf extracts on osteogenic induction of rat bone marrow mesenchymal stem cells (BMSCs) following peroxidative damage and to explore the underlying mechanisms.

Methods: Conditioned medium was used to induce osteogenic differentiation of BMSCs, which were treated with HO, Moringa oleifera leaf extracts-containing serum, or the phosphatidyl inositol-3 kinase (PI3K) inhibitor wortmannin, alone or in combination. Cell viability was measured using the MTT assay. Cell cycle was assayed using flow cytometry. Expression levels of Akt, phosphorylated (p)Akt, Foxo1, and cleaved caspase-3 were analyzed using western blot analysis. The mRNA levels of osteogenesis-associated genes, including alkaline phosphatase (ALP), collagen І, osteopontin (OPN), and Runx2, were detected using qRT-PCR. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and ALP activity were detected using commercially available kits. Osteogenic differentiation capability was determined using alizarin red staining.

Results: During osteogenic induction of rat BMSCs, HO reduced cell viability and proliferation, inhibited osteogenesis, increased ROS and MDA levels, and decreased SOD and GSH-PX activity. HO significantly reduced pAkt and Foxo1 expression, and increased cleaved caspase-3 levels in BMSCs. Additional treatments with Moringa oleifera leaf extracts partially reversed the HO-induced changes. Wortmannin partially attenuated the effects of Moringa oleifera leaf extracts on protein expression of Foxo1, pAkt, and cleaved caspase-3, as well as mRNA levels of osteogenesis-associated genes.

Conclusion: Moringa oleifera leaf extracts ameliorate peroxidative damage and enhance osteogenic induction of rat BMSCs by activating the PI3K/Akt/Foxo1 pathway.
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http://dx.doi.org/10.1186/s13018-021-02284-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896384PMC
February 2021

Integrated analysis of transcriptome and proteome to explore the genes related to steroid-induced femoral head necrosis.

Exp Cell Res 2021 Apr 7;401(1):112513. Epub 2021 Feb 7.

Department of Orthopaedic, The First Affiliated Hospital of Soochow University, No. 899, Pinghai Road, Suzhou City, Jiangsu Province, 215006, China.

Purpose: Femoral head necrosis (FHN) is a common disease of hip. However, the pathogenesis of FHN is not well understood. This study attempted to explore the potentially important genes and proteins involved in FHN.

Methods: We integrated the transcriptomic and proteomic methods to quantitatively screen the differentially expressed genes (DEGs) and proteins (DEPs) between Control and FHN groups. Gene ontology (GO) terms and KEGG pathway enrichment analysis were used to assess the roles of DEGs and DEPs. qRT-PCR and western blot were performed to verify the key genes/proteins in FHN. CCK-8 assay was performed to measure cell viability. The protein expression of Bax and Bcl-2 were used to evaluate cell apoptosis.

Results: Transcriptome and proteome studies indicated 758 DEGs and 1097 DEPs between Control and FHN groups, respectively. Cell division, extracellular exosome, and serine-type endopeptidase activity were the most common terms in biological process (BP), cellular component (CC), and molecular function (MF) enrichment, respectively. DEPs were mainly enriched in cellular process, cell, and binding for BP, CC, and MF categories, respectively. DEGs were mainly involved in PI3K-Akt pathway and DEPs were mainly focused in glycolysis/gluconeogenesis pathway. Notably, 14 down-regulated and 22 up-regulated genes/proteins were detected at both the transcript and protein level. LRG1, SERPINE2, STMN1, COL14A1, SLC37A2, and MMP2 were determined as the key genes/proteins in FHN. SERPINE2/STMN1 overexpression increased viability and decreased apoptosis of dexamethasone-treated MC3T3-E1 cells.

Conclusions: Our study investigated some pivotal regulatory genes/proteins in the pathogenesis of FHN, providing novel insight into the genes/proteins involved in FHN.
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http://dx.doi.org/10.1016/j.yexcr.2021.112513DOI Listing
April 2021

Cytogenetic characteristics of 665 patients with myelodysplastic syndrome in China: A single-center report.

Oncol Lett 2021 Feb 17;21(2):126. Epub 2020 Dec 17.

Department of Hematology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, P.R. China.

The karyotype is highly important for diagnosis and prognosis in myelodysplastic syndrome (MDS). The objective of the present study was to investigate the cytogenetic characteristics of patients with MDS in China. The karyotypes of 665 Chinese patients with MDS were analyzed, and it was identified that 298 cases (298/665, 44.8%) had abnormal karyotypes. Among the 298 patients with abnormal karyotypes, the 75 patients with trisomy 8 (+8) constituted the most common subset (75/298, 25.2%). The incidence of abnormal karyotypes was significantly higher in patients who were ≥51 years old compared with those <51 years old, (54.8 vs. 34.7%, respectively; P<0.05). Based on World Health Organization (WHO) classification-based Prognostic Scoring System (WPSS) criteria, the incidence of poor-prognosis karyotypes was significantly higher (17.4 vs. 5.4%; P<0.05) in the older patient group, and based on the Revised International Prognostic Scoring System (IPSS-R) criteria, the incidence of poor-/very poor-prognosis karyotypes was also significantly higher (17.4 vs. 6.6%; P<0.05) in patients ≥51 years old compared with younger ones. Based on the WHO classification of MDS subtypes, the incidence of abnormal karyotypes in patients with high percentages of bone marrow (BM) blasts [excess blasts (EB)-I + EB-II, ≥5% blasts] was significantly higher than that in patients with low percentages of BM blasts (those with single lineage dysplasia + multilineage dysplasia, <5% blasts) (62.5 vs. 36.0%; P<0.05). The incidence of poor-prognosis karyotypes based on WPSS criteria was significantly higher in patients with high percentages of BM blasts than those with low percentages (22.0 vs. 6.9%, respectively; P<0.05), and the incidence of poor-/very poor-prognosis karyotypes based on IPSS-R criteria was also significantly higher (23.0 vs. 7.4%, respectively; P<0.05). These results demonstrate that +8 is the most common abnormal karyotype in Chinese patients with MDS. Age and the percentage of BM blasts are associated with the incidence of both abnormal karyotypes and karyotypes with poor prognosis. The results of cytogenetic abnormalities in this study will supplement the data on patients of MDS in China.
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http://dx.doi.org/10.3892/ol.2020.12387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798047PMC
February 2021

Effect of Simultaneous Integrated Boost Intensity Modulated Radiation Therapy (SIB-IMRT) and Non-Operative Strategy on Outcomes of Distal Rectal Cancer Patients with Clinically Positive Lateral Pelvic Lymph Node.

Cancer Manag Res 2021 22;13:537-546. Epub 2021 Jan 22.

Department of Radiation Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, Beijing 100142, People's Republic of China.

Background: We aimed to analyze the effect of simultaneous integrated boost intensity modulated radiation therapy (SIB-IMRT) and non-operative treatment on the clinical outcomes of distal rectal cancer patients exhibiting clinically positive lateral pelvic lymph nodes (LPLNs).

Methods: We reviewed the medical records of patients diagnosed as having distal rectal adenocarcinoma with clinically positive LPLNs (≥7 mm, with irregular borders or mixed signal intensity) using primary pelvic magnetic resonance imaging (MRI). These patients had received SIB-IMRT-based neoadjuvant chemoradiotherapy (NCRT) and non-operative treatment according to the heterogeneity of the disease or personal preference. Chi-square tests were used to compare data between the two groups. Progression-free survival (PFS) and local regrowth were evaluated using the Kaplan-Meier method.

Results: Between 2016 and 2019, we analyzed 75 patients diagnosed as having clinically positive LPLNs using primary MRI. SIB-IMRT was delivered to the planning positive LPLNs (PGTVn) at a total dose of 56-60 Gy. After NCRT, 23 patients underwent non-operative treatment. Among these patients, the median short axis of LPLNs was 8 mm (range: 7-21 mm). Fifteen patients were categorized into the mesorectal fascia (MRF)-positive group. The median follow-up duration for these patients was 19.8 months, and no patient exhibited LPLN regrowth. The 2-year PFS rate was 85.6% for non-operative patients, 74.6% for operative patients, and 90.0% for the pathological complete response (pCR) subgroup. Eighteen patients who underwent non-operative treatment were included in the clinical complete response (cCR) subgroup. The 2-year PFS and local regrowth rates in this group were similar to those in patients with clinically negative LPLN who achieved cCR. During NCRT, 21 (28.0%) patients experienced grade 2-3 acute reversible toxicity.

Conclusions: SIB-IMRT could eliminate metastases in LPLNs in a safe and effective manner, and non-operative strategies may be promising for cCR patients.
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http://dx.doi.org/10.2147/CMAR.S286796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837555PMC
January 2021

Effect of Qizhitongluo capsule on lower limb rehabilitation after stroke: A randomized clinical trial.

Pharmacol Res 2021 Mar 27;165:105464. Epub 2021 Jan 27.

Luohe Hospital of Chinese Medicine, No. 649 Jiao Tong Road, Luohe, 462000 Henan, China.

Background: An individual's level of lower limb motor function is associated with his or her disability level after stroke, and motor improvement may lead to a better prognosis and quality of life. Data from animal models show that Qizhitongluo (QZTL) capsule facilitates recovery after focal brain injury. We aimed to validate the efficacy and safety of the QZTL capsule for promoting lower limb motor recovery in poststroke patients.

Methods: In this randomized, multicenter, double-blind, placebo- and active-controlled trial from 13 sites in China, participants with ischemic stroke and Fugl-Meyer motor scale (FMMS) scores of <95 were eligible for inclusion. Patients were randomly assigned in a 2:1:1 ratio to the QZTL group, Naoxintong (NXT) group or placebo group for 12 weeks at 15-28 days after the onset of stroke. The primary outcome was the change in the Lower Limb FMMS (FMMS-LL) score from baseline over the 12-week intervention period.

Results: 622 participants were randomly assigned to the QZTL group (309), NXT group (159), or placebo group (154). The FMMS-LL score increased by 4.81 points (95 % CI, 4.27-5.35) in the QZTL group, by 3.77 points (95 % CI, 3.03-4.51) in the NXT group and by 3.00 points (95 % CI, 3.03-4.51) in the placebo group at week 12. The QZTL group showed significantly larger improvements compared with the placebo group at each interview from weeks 4-12 (difference, 0.89 [0.30,1.49] at week 4, P = 0.0032; difference, 1.83[1.01,2.66] at 90 days poststroke, P < 0.0001; difference, 1.81[0.88,2.74] at week 12, P = 0.0001).

Conclusion: The QZTL capsule is an effective treatment for lower limb motor impairment. The finding indicates that the QZTL capsule may be used as a potential new strategy for stroke rehabilitation.
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http://dx.doi.org/10.1016/j.phrs.2021.105464DOI Listing
March 2021

An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar.

BMC Plant Biol 2021 Jan 21;21(1):54. Epub 2021 Jan 21.

Beijing Agro-Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Sciences, No. 9, Shuguang Huayuan Middle Road, Haidian District, Beijing, 100097, People's Republic of China.

Background: Forest trees have important economic and ecological value. As a model tree, poplar has played a significant role in elucidating the molecular mechanisms underlying tree biology. However, a lack of mutant libraries and time-consuming stable genetic transformation processes severely limit progress into the functional characterization of poplar genes. A convenient and fast transient transformation method is therefore needed to enhance progress on functional genomics in poplar.

Methods: A total of 11 poplar clones were screened for amenability to syringe infiltration. Syringe infiltration was performed on the lower side of the leaves of young soil-grown plants. Transient expression was evaluated by visualizing the reporters β-glucuronidase (GUS) and green fluorescent protein (GFP). The experimental parameters of the syringe agroinfiltration were optimized based on the expression levels of the reporter luciferase (LUC). Stably transformed plants were regenerated from transiently transformed leaf explants through callus-induced organogenesis. The functions of Populus genes in secondary cell wall-thickening were characterized by visualizing lignin deposition therein after staining with basic fuchsin.

Results: We greatly improved the transient transformation efficiency of syringe Agrobacterium infiltration in poplar through screening for a suitable poplar clone from a variety of clones and optimizing the syringe infiltration procedure. The selected poplar clone, Populus davidiana × P. bolleana, is amenable to Agrobacterium syringe infiltration, as indicated by the easy diffusion of the bacterial suspension inside the leaf tissues. Using this technique, we localized a variety of poplar proteins in specific intracellular organelles and illustrated the protein-protein and protein-DNA interactions. The transiently transformed leaves could be used to generate stably transformed plants with high efficiency through callus induction and differentiation processes. Furthermore, transdifferentiation of the protoxylem-like vessel element and ectopic secondary wall thickening were induced in the agroinfiltrated leaves via the transient overexpression of genes associated with secondary wall formation.

Conclusions: The application of P. davidiana × P. bolleana in Agrobacterium syringe infiltration provides a foundation for the rapid and high-throughput functional characterization of Populus genes in intact poplar plants, including those involved in wood formation, and provides an effective alternative to Populus stable genetic transformation.
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http://dx.doi.org/10.1186/s12870-021-02833-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818742PMC
January 2021

Isolation, identification and genotyping of Candida albicans from Landes geese.

Transbound Emerg Dis 2021 Jan 8. Epub 2021 Jan 8.

College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.

In May 2018, Landes geese raised in Weifang, Shandong Province, China, developed a disease characterized by thickened oesophageal mucosa and white, round ulcers. Based on pathogen isolation and identification, differential culture and morphological observations, Candida albicans (C. albicans) was identified as the causative pathogen from the oesophagus of infected geese, and artificial infection experiments were then performed using the isolated strains. In experimental reproduction, the symptoms of infected geese were consistent with those of natural infection, and gosling morbidity and mortality were 75% and 60%, respectively. Re-isolation of the strain from the dead goslings confirmed C. albicans as the causative pathogen of oesophageal ulcers. We further performed internal transcribed space rDNA sequence analysis, ABC genotyping and multi-locus sequence typing analysis. We observed 100% sequence similarity between the two strains, designated as WFCL and WFLQ, which were isolated from different regions, with 100% homology between the strains isolated in the present study and the human-origin C. albicans strains isolated previously from China. The goose-origin strains isolated in this study and the human-origin C. albicans isolates were included in the same branch in phylogenetic trees analysis, indicating that the strain responsible for oesophageal ulcer in geese is closely related to human-origin C. albicans. In addition, based on eBURST analysis of sequence types, goose-origin C. albicans strains were relatively independent in terms of population evolution. To the best of our knowledge, this is the first detailed report on goose oesophageal ulceration caused by C. albicans infection in geese. Considering that C. albicans is an important zoonotic pathogen, this study provides a reference for further studies on avian C. albicans infections and is important for ensuring public health and safety.
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http://dx.doi.org/10.1111/tbed.13985DOI Listing
January 2021

Advance directives and end-of-life care: knowledge and preferences of patients with brain Tumours from Anhui, China.

BMC Cancer 2021 Jan 5;21(1):25. Epub 2021 Jan 5.

Department of Oncology, Hefei Cancer Hospital; Chinese Academy of Sciences, Hefei, 230031, Anhui, China.

Background: In Mainland China, advance directives (ADs) and end-of-life care for patients with tumours, especially patients with brain tumours who may have lost consciousness or the ability to speak at the early stage of their illness, have been poorly acknowledged. Thus, this study aimed to clarify the knowledge and preferences of ADs and end-of-life care in patients with brain tumours and to investigate predictors of patient preferences.

Methods: This was a population-based cross-sectional survey that was conducted via face-to-face interviews. Information on sociodemographic factors, brain tumour illness, knowledge and preferences of the advanced decisions and end-of-life care of the patients was collected.

Results: A total of 88.61% of participants had never heard of ADs, but 65.18% reported that they would like to make ADs. Knowledge of ADs, receiving surgical treatment or radiotherapy, being younger than 70 years old, being male, having educational qualifications of college or beyond, being childless, having medical insurance for nonworking or working urban residents and self-paying medical expenses were predictors of preference for making ADs. A total of 79.43% of participants wanted to discuss end-of-life arrangements with medical staff, and 63.29% of participants were willing to receive end-of-life care, even though it would not delay death. A total of 65.82% of patients with brain tumours wanted resuscitation, and as many as 45.45% of the patients thought that they did not need life support if they were in a persistent vegetative state. Brain primary tumours, being younger than 70 years old, male sex, educational qualification of junior middle school or below, having children, having new rural cooperative medical insurance and having medical expenses paid by children or spouses were predictors of choosing appropriate palliative care.

Conclusions: ADs and end-of-life care have been poorly acknowledged among patients with brain tumours in mainland China. Additional efforts should be encouraged amongst patients with primary brain tumours, those who are undergoing surgery and radiotherapy and those who have low socioeconomic status. A longitudinal and comprehensive study is encouraged to promote disease-specific ADs among Chinese patients with brain tumours.
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http://dx.doi.org/10.1186/s12885-020-07775-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786498PMC
January 2021

The PAD4 inhibitor GSK484 enhances the radiosensitivity of triple-negative breast cancer.

Hum Exp Toxicol 2021 Jul 23;40(7):1074-1083. Epub 2020 Dec 23.

Department of Oncology, The Second Nanning People's Hospital, Nanning, Guangxi, China.

Triple-negative breast cancer (TNBC) accounts for approximately 10-20% of all breast cancers and is one of the leading causes of mortality among females. Radiotherapy is essential during the treatment of breast cancer. Growing evidence has indicated that peptidyl arginine deiminase-4 (PAD4) inhibitor can alleviate the development of multiple cancers, including breast cancer, through inhibiting cell proliferation. GSK484 is considered to be a highly potent PAD4-selective inhibitors. However, the potential role and mechanism of GSK484 in TNBC remain unclear. In this study, we intended to explore the effects of GSK484 on the radiosensitivity of TNBC cell lines (MDA-MB-231 and BT-549). We found that the pretreatment of GSK484 enhanced irradiation (IR)-induced inhibitory effects on cell proliferation, migration and invasion. Besides, our findings revealed that GSK484 facilitated TNBC cell apoptosis. IR treatment-induced increase of the protein level of ATG5 and ATG7, and decrease of p62 protein level were countervailed by GSK484. In addition, GSK484 enhanced DNA damage induced by IR. Moreover, in vivo experiments demonstrated that the combined treatment of IR and GSK484 showed an obvious decline of tumor growth in contrast to IR-alone or GSK484-alone treatment. Overall, GSK484 may serve as a radiosensitizer of TNBC through inhibiting IR-induced autophagy.
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http://dx.doi.org/10.1177/0960327120979028DOI Listing
July 2021

Genetic characterization and pathogenicity of a divergent broiler-origin orthoreovirus causing arthritis in China.

Transbound Emerg Dis 2020 Dec 20. Epub 2020 Dec 20.

College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong Province, China.

Since 2013, there have been an increasing number of cases of arthritis in broilers caused by avian orthoreovirus (ARV) in China, and the virus remains highly virulent in chicks with high-level maternal antibodies. However, little information is available about the complete gene analysis and pathogenicity of the epidemic ARVs. In the study, the ARV strain (V-ARV-SD26) was isolated from broilers associated with arthritis in Shandong Province. To genetically characterize the ARV strain, the whole-genome sequencing was conducted by next-generation sequencing (NGS) technique. Sequence analysis demonstrated that V-ARV-SD26 might have acquired its current genomic composition through several homologous and, in case of the λC, μA and σB, divergent reassortment events. To further investigate the pathogenicity of the strain, 160 one-day-old Ross broilers with maternal antibodies were equally divided into four groups (foodpad-, eye mucosa- and intramuscular-inoculated groups and the negative control group), three experimental groups were inoculated separately with the low-dose virus fluid, and the negative control was equally inoculated with sterile PBS. The results showed that the symptoms of broilers in foodpad inoculation group were the most serious, while that of the eye mucosa infection group were the mildest. Meanwhile, the cloacal cotton swabs and organs were collected for qRT-PCR detection to evaluate the infection status. In conclusion, these findings indicate that V-ARV-SD26 is a divergent ARV strain, which provide experimental data for the prevention and control of newly emerged reovirus, and have a certain reference value for the preparation and evaluation of new vaccines.
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http://dx.doi.org/10.1111/tbed.13961DOI Listing
December 2020