Publications by authors named "Hongye Yang"

42 Publications

Effect of aging on color stability and bond strength of dual-cured resin cement with amine or amine-free self-initiators.

Dent Mater J 2021 Aug 19. Epub 2021 Aug 19.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedical Ministry of Education, School and Hospital of Stomatology, Wuhan University.

This study aimed to evaluate the effect of aging on the color stability and bond strengths of dual-cured resin cements containing amine or amine-free self-initiators. Three dual-cured and one light-cured resin cements were used. The covered (by lithium disilicate ceramic disks) and uncovered groups (n=10) were included. Color measurements were tested after 24 h, 10,000 and 20,000 thermal cycles (TCs). Micro-shear bond strengths (µSBS) were tested after 24 h, 10,000 and 20,000 TCs, and failure modes were analyzed (n=14). Two-way ANOVA and Tukey's test were implemented for color difference (ΔE*) and µSBS (α=0.05). The mean ΔE* difference was significant among groups (p<0.001). The lowest ΔE* values were obtained for dual-cured resin cement with amine-free self-initiators dual-cured cement after aging in all dual-cured resin cements, and the µSBS of the dual-cured resin cements on ceramic was significantly higher than that of the light-cured ones after aging (p<0.001).
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http://dx.doi.org/10.4012/dmj.2020-306DOI Listing
August 2021

An MSN-based synergistic nanoplatform for root canal biofilm eradication Fenton-enhanced sonodynamic therapy.

J Mater Chem B 2021 Jul 29. Epub 2021 Jul 29.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.

The validity and biocompatibility of irrigating agents are imperative for the success of root canal therapy. The imperfections in the currently available irrigants highlight the fact that more advanced technologies and strategies are required for complete disinfection in endodontic treatments. In the present study, a Fenton reaction-enhanced antimicrobial sonodynamic therapy (SDT) platform was fabricated for root canal disinfection. Firstly, mesoporous silica nanoparticles (MSNs) were synthesized, grafted with an amino group and then conjugated with sonosensitizer protoporphyrin IX (PpIX). Iron ions were then anchored ([email protected]) to initiate a Fenton reaction. Nanoparticle characterization by size and zeta potential measurements, scanning electron microscopy, transmission electron microscopy and thermogravimetric analysis confirmed that the platform was successfully developed. Reactive oxygen species (ROS) generation assessment, methylene blue degradation and electron spin resonance assays illustrated upon ultrasound (US) irradiation, that augmented ROS, can be produced by US activated PpIX and iron mediated Fenton reactions from low concentration H2O2 (0.01%). In vitro anti-Enterococcus faecalis efficacy was demonstrated by growth curve and colony forming unit measurements. Confocal laser scanning microscopy and scanning electron microscopy observations illustrated the effectiveness of the platform on in situ biofilm eradication in root canal. Owing to the stronger oxidizing capability and short lifetime of ROS, the Fenton reaction-enhanced SDT can induce detrimental oxidative damage to bacteria upon activation of US while avoiding nonspecific toxicity to cells, which was verified by cytotoxicity evaluations using CCK-8 assay and morphology observation of MC3T3-E1 cells. Compared to commonly used NaClO, this nanoplatform displayed desirable anti-bacterial, anti-biofilm abilities and better biocompatibility. These results highlight that the integrated [email protected] + US + H2O2 platform is a promising candidate for US enhanced root canal irrigation and disinfection.
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http://dx.doi.org/10.1039/d1tb01031jDOI Listing
July 2021

In vitro evaluation of the anti-proteolytic and cross-linking effect of mussel-inspired monomer on the demineralized dentin matrix.

J Dent 2021 Aug 10;111:103720. Epub 2021 Jun 10.

Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 2nd Floor, Prince Philip Dental Hospital 34 Hospital Road, Sai Ying Pun, Hong Kong, PR China. Electronic address:

Objectives: To evaluate the anti-proteolytic and cross-linking effect of N-(3,4-dihydroxyphenethyl)methacrylamide (DMA) on the demineralized dentin matrix in vitro.

Methods: Four experimental solutions were selected: 50% ethanol/water solution (Control); 1, 5, and 10 mmol/L DMA dissolved in 50% ethanol/water solution. Sound human molars were sectioned to produce dentin beams with dimension of 1×1×6 mm. The dentin beams were demineralized with 10% phosphoric acid for 8 h to remove the apatite. The demineralized specimens were randomly separated into four groups and immersed in the four experimental solutions for 1 h. After the treatment, the ultimate tensile strength (UTS), loss of dry mass and the release of hydroxyproline by storing the treated specimens in 0.1 mg/mL collagenase solution for 24 h were assessed. The swelling ratio of another ten specimens from each group were evaluated. The interaction between DMA with dentin matrix was observed under Field Emission Scanning Electron Microscopy (FESEM). UTS data was analyzed by two-way ANOVA followed by Tukey test, and the other data was analyzed by one-way ANOVA followed by Tukey test (α = 0.05).

Results: The two-way ANOVA factors, different solutions (p < 0.001), collagenase degradation (p < 0.001) and their interactions (p < 0.001) all significantly affected the UTS. The 10 mM DMA treatment significantly decreased the percentage of loss of dry mass, release of hydroxyproline and swelling ratio of demineralized dentin matrix compared to other treatment groups (p < 0.05). The FESEM observation depicted that with increasing concentration of DMA, the structure of dentin matrix was protected and the porosity within dentin collagen network was decreased.

Conclusions: The treatment by 10 mM DMA/ethanol solution for 1 hour is capable of enhancing the mechanical properties of demineralized dentin matrix against collagenase degradation and may be clinically useful to improve the durability of hybrid layer.

Clinical Significance: The 10 mM DMA/ethanol primer may offer an alternative choice for dentists to strengthen the mechanical properties of demineralized dentin matrix and resist its degradation by collagenase.
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http://dx.doi.org/10.1016/j.jdent.2021.103720DOI Listing
August 2021

The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition.

Int J Nanomedicine 2021 27;16:3041-3057. Epub 2021 Apr 27.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.

Background: The dentin exposure always leads to dentin hypersensitivity and/or caries. Given the dentin's tubular structure and low mineralization degree, reestablishing an effective biobarrier to stably protect dentin remains significantly challenging. This study reports a versatile dentin surface biobarrier consisting of a mesoporous silica-based epigallocatechin-3-gallate (EGCG)/nanohydroxyapatite delivery system and evaluates its stability on the dentinal tubule occlusion and the () biofilm inhibition.

Materials And Methods: The mesoporous delivery system was fabricated and characterized. Sensitive dentin discs were prepared and randomly allocated to three groups: 1, control group; 2, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) group; and 3, the mesoporous delivery system group. The dentin permeability, dentinal tubule occlusion, acid and abrasion resistance, and biofilm inhibition were determined for 1 week and 1 month. The in vitro release profiles of EGCG, Ca, and P were also monitored.

Results: The mesoporous delivery system held the ability to sustainably release EGCG, Ca, and P and could persistently occlude dentinal tubules with acid and abrasion resistance, reduce the dentin permeability, and inhibit the biofilm formation for up to 1 month compared with the two other groups. The system provided prolonged stability to combat oral adverse challenges and served as an effective surface biobarrier to protect the exposed dentin.

Conclusion: The establishment of the dentin surface biobarrier consisting of a mesoporous delivery system indicates a promising strategy for the prevention and the management of dentin hypersensitivity and caries after enamel loss.
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http://dx.doi.org/10.2147/IJN.S290254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088303PMC
May 2021

Effect of universal adhesive and silane pretreatment on bond durability of metal brackets to dental glass ceramics.

Eur J Oral Sci 2021 04;129(2):e12772

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

This study aimed to investigate the effect of universal adhesive and silane pretreatment on the bond durability of metal brackets to dental glass ceramics. Eighty lithium disilicate glass ceramic specimens were randomly assigned to one of four groups (n = 20) defined by the pretreatment and adhesive used: (i) Adper Single Bond 2; (ii) silane + Adper Single Bond 2; (iii) Single Bond Universal; and (iv) silane +Single Bond Universal. Maxillary central incisor metal brackets were bonded on the ceramic surfaces with resin composite. A shear bond strength test was conducted after 24 h of water storage and after 10,000 thermocycles. Adhesive remnant index scoring and field-emission scanning electron microscopy were performed to determine adhesives remaining on the ceramic surfaces and the ceramic ultrastructure following bracket debonding, respectively. After 10,000 thermocycles, specimens treated with Single Bond Universal preserved an appropriate bond strength between brackets and glass ceramics and showed minimum ceramic surface damage following bracket debonding, which was not the case in the other three groups. The application of a silane-containing universal adhesive without silane pretreatment achieves adequate durability of the bond of metal brackets to dental glass ceramics and allows safe debonding, which may aid in optimizing the effectiveness for orthodontic treatment.
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http://dx.doi.org/10.1111/eos.12772DOI Listing
April 2021

Epigallocatechin-3-gallate/nanohydroxyapatite platform delivery approach to adhesive-dentin interface stability.

Mater Sci Eng C Mater Biol Appl 2021 Mar 29;122:111918. Epub 2021 Jan 29.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address:

Current adhesive techniques allow clinicians to bond composite resin to dentin for esthetic restoration of defected tooth. However, a vulnerable adhesive-dentin interface remains clinically challenging resulting in frequent replacement of the restorations. The inappropriate management of exposed dentin plays a major role in jeopardizing the bond stability of the adhesive-dentin interface. To overcome this problem, this paper highlights an epigallocatechin-3-gallate/nanohydroxyapatite (EGCG/nHAp) platform (mesoporous silica-based) delivery approach to the adhesive-dentin interface and investigates its effectiveness on dentin bonding durability. Microtensile bond strength, interfacial nanoleakage, and in situ zymography were determined. The inhibition of Streptococcus mutans (S. mutans) biofilm formation along the adhesive-dentin interface was assessed by confocal-laser scanning microscopy, colony forming units counts, and field-emission scanning electron microscopy. Results revealed that applying the EGCG/nHAp delivery platform on exposed dentin could preserve the dentin bond strength and reduce interfacial nanoleakage after collagenase ageing; moreover, it could inactivate the activity of matrix metalloproteinase within the hybrid layer and inhibit the adhesion and biofilm formation of S. mutans. The proposed approach demonstrates great potential for stabilizing the adhesive-dentin interface to improve dentin bonding durability and prevent secondary caries progression, thereby indicating a promising strategy to prolong the service life of dental restorations.
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http://dx.doi.org/10.1016/j.msec.2021.111918DOI Listing
March 2021

Effects of Desensitizers on Adhesive-Dentin Bond Strength: A Systematic Review and Meta-analysis.

J Adhes Dent 2021 ;23(1):7-19

Purpose: To systematically review in vitro studies that evaluated adhesive-dentin bond strength with or without the addition of desensitizers.

Materials And Methods: A search was conducted in four databases (PubMed, Embase, Web of Science, and Cochrane Library) without publication date or language limits in accordance with the Preferred Reporting Items for Systematic Reviews (PRISMA). Two reviewers selected the studies, extracted data, and assessed the risk of bias independently. The inclusion criterion was the evaluation of the effect of immediate or aged bond strength after applying desensitizer in the bonding step. The risk of bias was assessed following the method used by Sarkis-Onofre et al.41 Meta-analysis was conducted using Review Manager 5.3.

Results: A total of 1636 articles were found using the search strategy above; 59 articles were selected for full-text analysis, and 32 were systematically reviewed, with 18 considered in the meta-analysis. Results showed a significant difference among various kinds of desensitizers. The subgroup comparison showed high heterogeneity among the different kinds of desensitizers. The use of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and arginine-CaCO3 showed no negative effect on the immediate bond strength, whereas other desensitizers showed some influence.

Conclusion: Different desensitizers have various effects on microtensile bond strength. Desensitizers may be selected based on the bond strength and especially used to relieve tooth sensitivity after tooth preparation.
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http://dx.doi.org/10.3290/j.jad.b916811DOI Listing
February 2021

Injectable chitin hydrogels with self-healing property and biodegradability as stem cell carriers.

Carbohydr Polym 2021 Mar 30;256:117574. Epub 2020 Dec 30.

College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-based Medical Materials, Wuhan University, Wuhan, 430072, China. Electronic address:

To meet the demands of various therapeutic tasks, injectable hydrogels with tunable mechanical properties and degradability are highly desired. Herein, we developed an injectable chitin hydrogel system with well-manipulated mechanical properties and degradability through dynamic acylhydrazone crosslinking catalyzed by 4-amino-DL-phenylalanine (Phe-NH). The mechanical properties and degradability of the hydrogels could be easily adjusted by varying the solid content, while their gelation time could be maintained at a constant level (∼130 s) by altering Phe-NH content, thereby ensuring the good injectability of hydrogels. Moreover, the chitin hydrogels showed excellent self-healing capacity with a healing efficiency up to 95 %. Owing to their superior biocompatibility and biodegradability, the chitin hydrogels could support the proliferation and multi-potent differentiations of rat bone marrow-derived stem cells, serving as a beneficial 3D scaffold for stem cell encapsulation and delivery. This work provides a promising injectable delivery vehicle of therapeutic drugs or cells for tissue regenerative medicine.
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http://dx.doi.org/10.1016/j.carbpol.2020.117574DOI Listing
March 2021

Effect of apigenin on surface-associated characteristics and adherence of Streptococcus mutans.

Dent Mater J 2020 Dec 7;39(6):933-940. Epub 2020 Oct 7.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University.

Apigenin is a type of flavonols that exhibits anti-caries properties. Bacterial adherence is the initial step in the forming of a stable biofilm that leads to caries. Bacterial adherence is affected by surface characteristics, including hydrophobicity and bacterial aggregation. However, the effect of apigenin on surface characteristics of cariogenic bacteria has not been reported. We aimed to examine the effects of apigenin on adherence and biofilm formation of Streptococcus mutans UA159. Hydrophobicity and bacterial aggregation, pac and gbpC gene expressions, and cytotoxicity on human dental pulp cells were also determined. Apigenin significantly inhibited the adherence and biofilm formation of S. mutans. Hydrophobicity decreased, whereas the aggregation rate was significantly increased compared with the control. Apigenin significantly suppressed pac and gbpC gene expressions. Apigenin exhibited acceptable biocompatibility on hDPCs. Thus, apigeinin may affect adherence and biofilm formation by altering the surface properties of S. mutans without obvious adverse effect on hDPCs.
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http://dx.doi.org/10.4012/dmj.2019-255DOI Listing
December 2020

Effects of resveratrol/ethanol pretreatment on dentin bonding durability.

Mater Sci Eng C Mater Biol Appl 2020 Sep 23;114:111000. Epub 2020 Apr 23.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, China. Electronic address:

To determine the effects of resveratrol/ethanol solution on the durability of resin-dentin bonding interfaces. Sixty-four non-caries third molars were randomly divided into four groups (n = 16) after sectioning, and then pretreated with one of the following concentrations of resveratrol/ethanol solutions: 0 (control group), 1, 10 and 20 mg/mL, followed by a universal adhesive and resin composites. All microtensile samples were divided into three subgroups: immediate group, collagenase ageing group and thermocycled group. The microtensile bond strength (MTBS), failure modes, interfacial nanoleakage and in situ zymography were measured, whereas the inhibitory effects of pretreated dentin slices on S. mutans biofilms were determined by confocal laser scanning microscopy and MTT assay. The results indicated that bonding strength was not only influenced by pretreatment factors (P < 0.05) but also ageing factors (P < 0.05). Regardless of collagenase ageing or thermocycling, the 10 mg/mL resveratrol/ethanol pretreatment group presented significantly higher (P < 0.05) MTBS and lower (P < 0.05) expression of nanoleakage than the control group, showed better inhibitory effect of matrix metalloproteinases and S. mutans activity with acceptable cytotoxicity. Meanwhile, cohesive failure in dentin decreased gradually with increasing resveratrol concentration. Therefore, the resveratrol/ethanol solution had the potential to serve as a versatile dentin primer, which can effectively improve dentin bonding durability and prevent secondary caries.
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http://dx.doi.org/10.1016/j.msec.2020.111000DOI Listing
September 2020

New perspective to improve dentin-adhesive interface stability by using dimethyl sulfoxide wet-bonding and epigallocatechin-3-gallate.

Dent Mater 2020 11 14;36(11):1452-1463. Epub 2020 Sep 14.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address:

Objectives: To determine whether dentin-adhesive interface stability would be improved by dimethyl sulfoxide (DMSO) wet-bonding and epigallocatechin-3-gallate (EGCG).

Methods: Etched dentin surfaces from sound third molars were randomly assigned to five groups according to different pretreatments: group 1, water wet-bonding (WWB); group 2, 50% (v/v) DMSO wet-bonding (DWB); groups 3-5, 0.01, 0.1, and 1 wt% EGCG-incorporated 50% (v/v) DMSO wet-bonding (0.01%, 0.1%, and 1%EGCG/DWB). Singlebond universal adhesive was applied to the pretreated dentin surfaces, and composite buildups were constructed. Microtensile bond strength (μTBS) and interfacial nanoleakage were respectively examined after 24 h water storage or 1-month collagenase ageing. In situ zymography andStreptococcus mutans (S. mutans) biofilm formation were also investigated.

Results: After collagenase ageing, μTBS of groups 4 (0.1%EGCG/DWB) and 5 (1%EGCG/DWB) did not decrease (p > 0.05) and was higher than that of the other three groups (p < 0.05). Nanoleakage expression of groups 4 and 5 was less than that of the other three groups (p < 0.05), regardless of collagenase ageing. Metalloproteinase activities within the hybrid layer in groups 4 and 5 were suppressed. Furthermore, pretreatment with 1%EGCG/DWB (group 5) efficiently inhibited S. mutans biofilm formation along the dentin-adhesive interface.

Significance: This study suggested that the synergistic action of DMSO wet-bonding and EGCG can effectively improve dentin-adhesive interface stability. This strategy provides clinicians with promising benefits to achieve desirable dentin bonding performance and to prevent secondary caries, thereby extending the longevity of adhesive restorations.
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http://dx.doi.org/10.1016/j.dental.2020.08.009DOI Listing
November 2020

Deep learning detection of prostate cancer recurrence with F-FACBC (fluciclovine, Axumin®) positron emission tomography.

Eur J Nucl Med Mol Imaging 2020 12 17;47(13):2992-2997. Epub 2020 Jun 17.

Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Stanford University, 300 Pasteur Dr, Stanford, CA, 94305, USA.

Purpose: To evaluate the performance of deep learning (DL) classifiers in discriminating normal and abnormal F-FACBC (fluciclovine, Axumin®) PET scans based on the presence of tumor recurrence and/or metastases in patients with prostate cancer (PC) and biochemical recurrence (BCR).

Methods: A total of 251 consecutive F-fluciclovine PET scans were acquired between September 2017 and June 2019 in 233 PC patients with BCR (18 patients had 2 scans). PET images were labeled as normal or abnormal using clinical reports as the ground truth. Convolutional neural network (CNN) models were trained using two different architectures, a 2D-CNN (ResNet-50) using single slices (slice-based approach) and the same 2D-CNN and a 3D-CNN (ResNet-14) using a hundred slices per PET image (case-based approach). Models' performances were evaluated on independent test datasets.

Results: For the 2D-CNN slice-based approach, 6800 and 536 slices were used for training and test datasets, respectively. The sensitivity and specificity of this model were 90.7% and 95.1%, and the area under the curve (AUC) of receiver operating characteristic curve was 0.971 (p < 0.001). For the case-based approaches using both 2D-CNN and 3D-CNN architectures, a training dataset of 100 images and a test dataset of 28 images were randomly allocated. The sensitivity, specificity, and AUC to discriminate abnormal images by the 2D-CNN and 3D-CNN case-based approaches were 85.7%, 71.4%, and 0.750 (p = 0.013) and 71.4%, 71.4%, and 0.699 (p = 0.053), respectively.

Conclusion: DL accurately classifies abnormal F-fluciclovine PET images of the pelvis in patients with BCR of PC. A DL classifier using single slice prediction had superior performance over case-based prediction.
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http://dx.doi.org/10.1007/s00259-020-04912-wDOI Listing
December 2020

Biocompatible cellulose-based supramolecular nanoparticles driven by host-guest interactions for drug delivery.

Carbohydr Polym 2020 Jun 3;237:116114. Epub 2020 Mar 3.

College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-based Medical Materials, Wuhan University, Wuhan 430072, China. Electronic address:

To extend the applications of natural products in nanomedicine, novel cellulose-based supramolecular nanoparticles (SNPs) were fabricated via a host-guest driven self-assembly strategy here. The adamantane-grafted carboxyethyl hydroxyethyl cellulose and β-cyclodextrin-grafted glycerol ethoxylate were synthesized to self-assemble into the SNPs. Furthermore, doxorubicin (DOX)-functionalized β-cyclodextrin was encapsulated into SNPs via an in situ co-assembly process to generate DOX-loaded SNPs (DOX-SNPs). The SNPs exhibited a quasi-spherical morphology with an average diameter of ∼25 nm. The DOX-SNPs with relatively larger diameter possessed a high DOX loading efficiency (∼94 %) and the pH-responsive drug release behaviors, which made them suitable as a drug delivery system. In vitro cytotoxicity assays demonstrated the excellent cytocompatibility of SNPs and the efficient inhibition of Hela cell proliferation of DOX-SNPs. Moreover, the DOX-SNPs could effectively enter Hela cells via endocytosis and release DOX under endo/lysosome pH. Thus, this nanocarrier has promising translational potential in cancer therapy and personalized nanomedicine.
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http://dx.doi.org/10.1016/j.carbpol.2020.116114DOI Listing
June 2020

In vitro remineralization of enamel white spot lesions with a carrier-based amorphous calcium phosphate delivery system.

Clin Oral Investig 2020 Jun 8;24(6):2079-2089. Epub 2019 Nov 8.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Rd, Wuhan, 430079, China.

Objectives: To achieve in vitro remineralization of enamel white spot lesions (WSLs) via a mesoporous delivery system of amorphous calcium phosphate (ACP) precursors.

Materials And Methods: Amine-functionalized expanded pore mesoporous silica (aMSN) was loaded with polyacrylic acid-stabilized amorphous calcium phosphate (PAA-ACP) to develop a carrier-based delivery system ([email protected]). Thirty-six artificial WSLs samples were created and randomly assigned to three treatments: artificial saliva solution (negative control, n = 12), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) slurry (n = 12), and [email protected] slurry (n = 12). Surface microhardness, Raman intensity, and color were measured before/after artificial demineralization and after remineralization treatments to evaluate the remineralization level of each sample. SEM images were taken on the surface and cross-section of samples to observe microstructure changes.

Results: The surface microhardness recovery ratio (%SMHRR), Raman intensity change ratio (%ICR), and color recovery ratio (%CRR) were not significantly different between CPP-ACP and [email protected] groups (P > 0.05), but both of them had significantly higher %SMHRR, %ICR, and %CRR values than negative control (P < 0.01). SEM images showed that apparent enamel prism imprints and inter-prism gaps in negative control were masked by mineral deposition in the [email protected] and CPP-ACP groups.

Conclusions: [email protected] has an ability to remineralize enamel WSLs.

Clinical Relevance: The carrier-based amorphous calcium phosphate delivery system has great potential to serve as a remineralizing agent for the treatment of WSLs.
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http://dx.doi.org/10.1007/s00784-019-03073-xDOI Listing
June 2020

Combination of baicalein and ethanol-wet-bonding improves dentin bonding durability.

J Dent 2019 11 3;90:103207. Epub 2019 Oct 3.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address:

Objectives: This study aimed to investigate the potential of baicalein combined with ethanol-wet bonding (EWB) in improving dentin bonding durability.

Methods: Sixty caries-free human third molars were randomly allocated into four groups and pretreated with solutions after sectioning and polishing. The pretreatments were prepared via dissolving baicalein in ethanol at concentrations of 0, 0.01%, 0.05% and 0.1% (w/v). Microtensile bond strength (MTBS) test, failure mode analysis and interfacial nanoleakage evaluation were conducted immediately or after thermocycling or 1 month of collagenase aging. In situ zymography, contact angle, antibacterial activity and bioactivity were comprehensively assessed.

Results: Results demonstrated that the three experimental groups exhibited higher MTBS and lower nanoleakage expression regardless of aging. MMP activity within hybrid layer and Streptococcus. mutans biofilm formation were inhibited in the experimental groups in a dose-dependent manner. Baicalein also reduced reactive oxygen species (ROS) expression in human dental pulp cells and resisted adhesive-induced cytotoxicity. Baicalein exhibited remarkable capabilities at concentrations higher than 0.05% (w/v).

Conclusion: Baicalein is a prospective candidate as bioactive dentin bonding agent. Combined with EWB, baicalein may form a functional bonding interface, thereby enhancing dentin bond strength and durability.

Significance: Joint efforts by baicalein and EWB provides a novel therapeutic strategy for obtaining ideal adhesive-dentin interface and prolonging the longevity of restorations.
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http://dx.doi.org/10.1016/j.jdent.2019.103207DOI Listing
November 2019

NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro.

Int J Nanomedicine 2019 23;14:787-798. Epub 2019 Jan 23.

Center for Smart Materials and Devices, State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, Hubei, China,

Purpose: In this study, we aimed to develop a unique -acetyl cysteine (NAC)-loaded polylactic-co-glycolic acid (PLGA) electrospun system with separate compartments for the promotion of osteogenesis.

Materials And Methods: We first prepared solutions of NAC-loaded mesoporous silica nanoparticles (MSNs), PLGA, and NAC in , -dimethylformamide and tetrahydrofuran for the construction of the electrospun system. We then fed solutions to a specific injector for electrospinning. The physical and chemical properties of the scaffold were characterized through scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The release of NAC and Si from different PLGA scaffolds was estimated. The cell viability, cell growth, and osteogenic potential of rat bone marrow-derived stroma cell (rBMSCs) on different PLGA scaffolds were evaluated through MTT assay, live/dead staining, phalloidin staining, and Alizarin red staining. The expression levels of osteogenic-related markers were analyzed through real-time PCR (qRT-PCR).

Results: NAC was successfully loaded into MSNs. The addition of MSNs and NAC decreased the diameters of the electrospun fibers, increased the hydrophilicity and mechanical property of the PLGA scaffold. The release kinetic curve indicated that NAC was released from (PLGA + NAC)/([email protected]) in a biphasic pattern, that featured an initial burst release stage and a later sustained release stage. This release pattern of NAC encapsulated on the (PLGA + NAC)/([email protected]) scaffolds enabled to prolong the high concentrations of release of NAC, thus drastically affecting the osteogenic differentiation of rBMSCs.

Conclusion: A PLGA electrospun scaffold was developed, and MSNs were used as separate nanocarriers for recharging NAC concentration, demonstrating the promising use of (PLGA + NAC)/([email protected]) for bone tissue engineering.
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http://dx.doi.org/10.2147/IJN.S183233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361317PMC
March 2019

Current Enamel Remineralization Therapies Have Limited Effects on Postorthodontic White Spot Lesions.

J Evid Based Dent Pract 2018 12 16;18(4):339-342. Epub 2018 Oct 16.

Article Title And Bibliographic Information: Enamel remineralization therapies for treating postorthodontic white spot lesions: a systematic review. Fernández-Ferrer L, Vicente-Ruíz M, García-Sanz V, Montiel-Company JM, Paredes-Gallardo V, Almerich-Silla JM, Bellot-Arcís C. J Am Dent Assoc 2018; 149(9):778-86.e2.

Source Of Funding: No financial support was reported.

Type Of Study/design: Systematic review.
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http://dx.doi.org/10.1016/j.jebdp.2018.10.002DOI Listing
December 2018

A novel thermostable β-1,3-1,4-glucanase from Thermoascus aurantiacus and its application in oligosaccharide production from oat bran.

Carbohydr Res 2018 Nov 5;469:31-37. Epub 2018 Sep 5.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. Electronic address:

Fermentation conditions for β-1,3-1,4-glucanase (TaGlu34) production in submerged culture by a thermophilic fungus, Thermoascus aurantiacus CAU830 were optimized. The highest enzyme activity of 3741 U/mL was obtained, and the crude enzyme was purified to homogeneity with a purification fold of 7.3 and a recovery yield of 11.6%. The molecular mass of the purified enzyme was estimated to be approximately 34 kDa on SDS-PAGE. TaGlu34 was most active at pH 6.0 and 75 °C, respectively. It showed excellent thermostability with thermal denaturing half-lives of 209, 130 and 69 min at 50, 60 and 70 °C, respectively. TaGlu34 exhibited strict substrate specificity towards barley β-glucan (13,527 U/mg), oat β-glucan (12,502 U/mg) and lichenan (9225 U/mg), but displayed no activity on other tested polysaccharides including laminarin, xylan, pullulan, CMC and starch. TaGlu34 hydrolyzed barley β-glucan and lichenan to yield both mainly disaccharide and trisaccharide, suggesting that it should be an endo type β-1,3-1,4-glucanase. Furthermore, TaGlu34 efficiently degraded the β-glucan component in oat bran to produce mainly oligosaccharides with degrees of polymerization (DP) 3-5, with the highest conversion ratio of 47.1%. The high yield and excellent enzymatic properties of TaGlu34 may make it a good candidate in industries.
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http://dx.doi.org/10.1016/j.carres.2018.08.017DOI Listing
November 2018

Chlorhexidine-encapsulated mesoporous silica-modified dentin adhesive.

J Dent 2018 11 25;78:83-90. Epub 2018 Aug 25.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address:

Objectives: This work aims to explore the feasibility of chlorhexidine-encapsulated mesoporous silica ([email protected]) as a modifier of a commercial dental adhesive via the evaluation of physicochemical properties and antibacterial capabilities of adhesive-dentin interface.

Methods: Therapeutic adhesives were developed in the present study by incorporating [email protected] into a commercial adhesive at four mass fractions (0, 1, 5 and 10 wt.%). The antibacterial capability on Streptococcus mutans (S. mutans) biofilm, conversion degree, adhesive morphology, microtensile bond strength (MTBS) and nanoleakage expression were evaluated comprehensively.

Results: MTT and CLSM evaluation showed that [email protected] adhesive inhibits S. mutans biofilm growth, while CHX is released from the modified adhesive continuously. The incorporation of [email protected] did not affect immediate bond strength at the concentration of 1% and 5% (P >  0.05). Moreover, these bonds were mainly preserved in 5% [email protected] group after one month of collagenase ageing. Meanwhile, [email protected] adhesive groups exhibited similar nanoleakage distribution compared with the control.

Conclusion: This study showed that the 5% [email protected] adhesive achieved balance amongst unaffected immediate bonding strength, well-preserved bonds against collagenase ageing and effective inhibition of S. mutans biofilm growth.

Clinical Significance: [email protected] dentin adhesive can potentially extend the service life of adhesive restoration in clinic.
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http://dx.doi.org/10.1016/j.jdent.2018.08.012DOI Listing
November 2018

Influence of naringenin on the biofilm formation of Streptococcus mutans.

J Dent 2018 09 18;76:24-31. Epub 2018 Apr 18.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address:

Objectives: To evaluate the effect of naringenin on the biofilm formation of Streptococcus mutans (S. mutans), and to investigate its mechanisms of action and biological toxicity.

Methods: Minimum inhibitory concentrations, growth curves, and biofilm inhibition rates of naringenin were determined to assess its antimicrobial effect on S. mutans. The morphology of S. mutans and the structure of biofilm were observed by FESEM and CLSM. Bacterial aggregation, bacterial surface hydrophobicity, and real-time PCR for gtfB, gtfC, comD, comE, and luxS mRNA expression were assessed to preliminarily investigate the mechanisms of action. MTT test using human dental pulp cells (HDPCs) was also performed to investigate cytotoxicity.

Results: The S.mutans growth curves, FESEM, CLSM showed that both 100 and 200 μg/mL of naringenin obviously inhibited S. mutans growth and biofilm formation, increased S. mutans surface hydrophobicity, reduced bacterial aggregation, and downregulated the mRNA expression of gtfB, gtfC, comD, comE, and luxS. However, naringenin at 200 μg/mL slightly decreased the growths of HDPCs compared with 100 μg/mL.

Conclusion: Naringenin at 100 and 200 μg/mL suppressed the second (bacterial adhesion) and third stages (biofilm maturation) of S. mutans biofilm formation.

Clinical Significance: Naringenin is promising for dental clinic promotion to prevent the biofilm formation of S. mutans, serving as a safe anti-caries agent at an appropriate concentration.
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http://dx.doi.org/10.1016/j.jdent.2018.04.013DOI Listing
September 2018

Attenuation of EGFL7 Expression Inhibits Growth Hormone-Producing Pituitary Adenomas Growth and Invasion.

Hum Gene Ther 2018 Apr 2. Epub 2018 Apr 2.

1 Beijing Neurosurgical Institute, Capital Medical University , Beijing, China.

Invasiveness of growth hormone-producing pituitary adenomas (GHPAs) causes difficulties in safe and complete adenoma removal during surgery and often leads to high recurrence. Epidermal growth factor-like domain 7 (EGFL7) has been shown to be able to promote tumor angiogenesis, growth, invasiveness, and metastasis through the Notch signaling pathway. It was previously demonstrated that EGFL7 was overexpressed in GHPAs. This study reports that EGFL7 and Notch2 (positive correlation with EGFL7) are overexpressed in invasive GHPA. A long-rank test (Kaplan-Meier method) shows that invasive GHPAs with EGFL7 strong expression results in reduced recurrence-free survival. Multivariate Cox regression analysis reveals that weak EGFL7 expression is an independent prognostic factor for recurrence-free survival. In addition, knockdown of EGFL7 expression suppresses proliferation and invasion of GH3 and GT1-1 cells in vitro. Moreover, attenuation of EGFL7 inhibits human GHPA growth in vivo. The data suggest that as a Notch agonist, EGFL7 may potentially be an appropriate novel molecular target for future development of GHPA medical therapy.
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http://dx.doi.org/10.1089/hum.2017.200DOI Listing
April 2018

Application of electrophoretic deposition to occlude dentinal tubules in vitro.

J Dent 2018 04 31;71:43-48. Epub 2018 Jan 31.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address:

Objectives: This study aims to apply electrophoretic deposition (EPD) for occlusion of dentinal tubules in vitro and investigate its effect on tubule occlusion and shear bond strength (SBS).

Methods: Charged mesoporous silica nanoparticles (MSNs) were synthesized and characterized through field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and Fourier transform infrared (FT-IR) spectroscopy analyses. Thirty-nine sensitive dentin specimens were modeled and assigned randomly to three groups with different treatments (n = 13 each): group 1, immersion in the MSN suspension; and groups 2 and 3, anodic EPD with the specimen on the negative and positive electrode respectively. The effect of dentinal tubule occlusion was evaluated by dentin permeability test (n = 10 each) and FESEM examination (n = 3 each). Moreover, 18 specimens were grouped (n = 6 each) and treated in the same method. A resin stick was bonded onto each of the specimen using a self-etch adhesive (single bond universal) for SBS testing.

Results: Negatively-charged MSNs were synthesized and characterized as small and well-dispersed particles. After the EPD treatment (group 3), the dentinal tubules were effectively occluded by MSNs, which infiltrated into the tubules at a depth of approximately 7-8 μm and tightly associated with the tubular inwalls. SBS was not significantly different among the three groups (P > 0.05).

Conclusions: Synthesized MSNs were deposited into dentinal tubules by EPD treatment without compromising dentin bond strength.

Clinical Significance: Application of EPD is a new approach for occlusion of dentinal tubules and exhibits potential in the study of dentin hypersensitivity.
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http://dx.doi.org/10.1016/j.jdent.2018.01.012DOI Listing
April 2018

Effects of calcium-containing desensitizers on the bonding stability of an etch-and-rinse adhesive against long-term water storage and pH cycling.

Dent Mater J 2018 Jan 23;37(1):122-129. Epub 2017 Nov 23.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedical Ministry of Education, School and Hospital of Stomatology, Wuhan University.

This study aimed to evaluate the effects of two calcium-containing desensitizing pastes on the bonding stability of an etch-and-rinse (E&R) adhesive to dentine. After dentine hypersensitivity model established, dentine surfaces were assigned one of the following pretreatment: Group 1, no desensitizer; Group 2, CPP-ACP; and Group 3, Novamin. Specimens were then bonded with an E&R adhesive. Beams from each tooth were randomly divided into three subgroups and then subjected to microtensile bond strength (MTBS) test after 24 h; 12 months of water storage; or 15 runs of pH cycling. Failure modes, nanoleakage, and tubule-occluding effectiveness were analyzed. Results showed that CPP-ACP- or Novamin-pretreated specimens mainly preserved the bonding strength after 12 months of water storage, while effective tubule occlusion could be observed. The results suggested that the calcium-containing desensitizers were compatible pretreatment for bonding with E&R adhesives to obtain reliable long-term bonding strength and prevention of post-operative sensitivity.
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http://dx.doi.org/10.4012/dmj.2017-006DOI Listing
January 2018

High-performance therapeutic quercetin-doped adhesive for adhesive-dentin interfaces.

Sci Rep 2017 08 15;7(1):8189. Epub 2017 Aug 15.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.

Almost half of dental restorations have failed in less than 10 years, and approximately 60% of practice time has been consumed to replace these dental restorations. As such, contemporary dentin adhesives should be modified to treat secondary caries and prevent the degradation of adhesive-dentin interfaces. To achieve this goal, we developed a versatile therapeutic adhesive in the present study by incorporating quercetin, which is a naturally derived plant extract, into a commercial adhesive at three concentrations (100, 500 and 1000 µg/mL). An unmodified adhesive served as a control. The antibacterial ability on Streptococcus mutans biofilm, conversion degree, microtensile bond strength, failure modes, in situ zymography, nanoleakage expression and cytotoxicity of quercetin-doped adhesive were comprehensively evaluated. Results showed that the quercetin-doped adhesive (500 µg/mL) preserved its bonding properties against collagenase ageing and inhibited the growth of S. mutans biofilm. Efficient bonding interface sealing ability, matrix metalloproteinase inhibition and acceptable biocompatibility were also achieved. Thus, a simple, safe and workable strategy was successfully developed to produce therapeutic adhesives for the extension of the service life of adhesive restorations.
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http://dx.doi.org/10.1038/s41598-017-08633-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5558009PMC
August 2017

Effects of Chlorhexidine-Encapsulated Mesoporous Silica Nanoparticles on the Anti-Biofilm and Mechanical Properties of Glass Ionomer Cement.

Molecules 2017 Jul 21;22(7). Epub 2017 Jul 21.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.

One of the primary causes for the failure of glass ionomer cement (GIC) is secondary caries. To enhance the anti-microbial performance of GIC without affecting its mechanical properties, chlorhexidine (CHX) was encapsulated in expanded-pore mesoporous silica nanoparticles (pMSN) to synthesize [email protected] [email protected] was added at three mass fractions (1%, 5%, and 10% ()) to GIC powder as the experimental groups. Pure GIC was set as the control group. The mechanical and anti-biofilm properties of GIC from each group were tested. The results demonstrated that CHX was successfully encapsulated on/into pMSN, and the encapsulating efficiency of CHX was 44.62% in [email protected] The anti-biofilm ability was significantly enhanced in all experimental groups ( < 0.001) compared with that in the control group. CHX was continuously released, and anti-biofilm ability was maintained up to 30 days. In addition, the mechanical properties (compressive strength, surface hardness, elastic modulus, water sorption, and solubility) of 1% () group were maintained compared with those in the control group ( > 0.05). In conclusion, adding 1% () [email protected] to GIC led to conspicuous anti-biofilm ability and had no adverse effect on the mechanical properties of this restorative material. This study proposes a new strategy for preventing secondary caries by using [email protected] GIC.
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http://dx.doi.org/10.3390/molecules22071225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152133PMC
July 2017

Development of Epigallocatechin-3-gallate-Encapsulated Nanohydroxyapatite/Mesoporous Silica for Therapeutic Management of Dentin Surface.

ACS Appl Mater Interfaces 2017 Aug 28;9(31):25796-25807. Epub 2017 Jul 28.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University , Wuhan 430079, China.

In dental clinic, unsatisfactory management of the dentin surface after dentin exposure often leads to the occurrence of dentin hypersensitivity and caries. Current approaches can occlude the tubules on the dentin surface to relieve dentin hypersensitivity; however, the blocked tubules are generally weak in combating daily tooth erosion and abrasion. Moreover, cariogenic bacteria, such as Streptococcus mutans, produce biofilm on the dentin surface, causing caries and compromising the tubules' sealing efficacy. To overcome this problem, the present study focused on establishing a versatile biomaterial, epigallocatechin-3-gallate-encapsulated nanohydroxyapatite/mesoporous silica nanoparticle ([email protected]@MSN), for therapeutic management of the dentin surface. The effectiveness of the biomaterial on dentinal tubule occlusion, including resistances against acid and abrasion, was evaluated by field-emission scanning electron microscopy (FESEM) and dentin permeability measurement. The inhibitory capability of the biomaterial on S. mutans biofilm formation was investigated by confocal laser scanning microscopy (CLSM), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony forming units (CFU) counts, and FESEM. Results demonstrated for the first time that the use of [email protected]@MSN on the dentin surface was capable of effectively occluding dentinal tubules, reducing dentin permeability, and achieving favorable acid- and abrasion-resistant stability. Furthermore, [email protected]@MSN held the capability to continuously release EGCG, Ca, and P, and significantly inhibit the formation and growth of S. mutans biofilm on the dentin surface. Thus, the development of [email protected]@MSN bridges the gap between multifunctional concept and dental clinical practice and is promising in providing dentists a therapeutic strategy for the management of the dentin surface to counter dentin hypersensitivity and caries.
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http://dx.doi.org/10.1021/acsami.7b06597DOI Listing
August 2017

Dimethyl Sulfoxide Wet-bonding Technique May Improve the Quality of Dentin Bonding.

J Adhes Dent 2017 Jun 8:229-237. Epub 2017 Jun 8.

Purpose: To investigate the effect of the dimethyl-sulfoxide wet-bonding technique on composite-dentin bonds and to explore its potential mechanism.

Materials And Methods: Thirty human third molars were segmented, ground, etched, and randomly divided into three groups according to the following pretreatments: 1. water; 2. ethanol; 3. 50% (v/v) dimethyl sulfoxide (DMSO). Then, Single Bond 2 was applied and composite buildups were constructed. After 24 h of water storage or 10,000 cycles of thermocycling, the microtensile bond strength (MTBS) and nanoleakage were measured. Contact angle measurement, Masson's trichrome staining, and in situ zymography were used to explore the possible action mechanism of DMSO on adhesive-dentin interfaces.

Results: DMSO pretreatment prevented the decline of thermocycled MTBS (p < 0.05) without affecting the immediate MTBS (p > 0.05) compared to the water wet-bonded group. Nanoleakage expression in the thermocycled DMSO wet-bonded group was also less than that in the thermocycled water-wet group (p < 0.05). Moreover, DMSO decreased the contact angle of the dentin surfaces (p < 0.05), reduced the amount of collagen exposure (p < 0.05), and decreased the collagenolytic activity in the hybrid layer (p < 0.05).

Conclusion: The 50% DMSO pretreatment was effective in increasing the wettability of the etched dentin surface, promoting the penetration of the adhesive monomer, and enhancing the stability of the dentin collagen at the adhesive- dentin interface. All these changes may lead to higher quality dentin bonds, suggesting that DMSO wet bonding is a viable alternative to improve the durability of dentin bonding.
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http://dx.doi.org/10.3290/j.jad.a38438DOI Listing
June 2017

Biochemical properties and application of a novel β-1,3-1,4-glucanase from Paenibacillus barengoltzii.

Food Chem 2017 Nov 27;234:68-75. Epub 2017 Apr 27.

Beijing Huiyuan Food & Beverage Co., Ltd., Beijing 101305, China.

A novel endo-β-1,3-1,4-glucanase gene (PbBglu16A) was cloned from Paenibacillus barengoltzii and heterogeneously expressed in Escherichia coli. The recombinant β-1,3-1,4-glucanase (PbBglu16A) was purified to homogeneity with a recovery yield of 78.6% and a specific activity of 431.8Umg. The molecular mass of PbBglu16A was estimated to be 44.0kDa by SDS-PAGE. The optimal pH and temperature of PbBglu16A were 6.0 and 55°C, respectively. The enzyme was stable within pH 3.5-9.0 and up to 55°C. PbBglu16A exhibited high substrate specificity towards barley β-glucan, oat β-glucan and lichenin. PbBglu16A showed an endo-type cleavage pattern and hydrolyzed endogenous enzyme-deactivated oat bran into β-gluco-oligosaccharides with a yield of 7.0%, which mainly consisted of trioligosaccharide and tetraoligosaccharide. Further, PbBglu16A could promote mashing with a reduced filtration time (14.0%) and viscosity (3.4%). Thus, PbBglu16A might be a promising candidate for the production of β-gluco-oligosaccharides and in brewing industry.
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http://dx.doi.org/10.1016/j.foodchem.2017.04.162DOI Listing
November 2017

Effect of silane pretreatment on the immediate bonding of universal adhesives to computer-aided design/computer-aided manufacturing lithium disilicate glass ceramics.

Eur J Oral Sci 2017 04 24;125(2):173-180. Epub 2017 Feb 24.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

The aim of this study was to investigate the effect of silane pretreatment on the universal adhesive bonding between lithium disilicate glass ceramic and composite resin. IPS e.max ceramic blocks etched with hydrofluoric acid were randomly assigned to one of eight groups treated with one of four universal adhesives (two silane-free adhesives and two silane-containing adhesives), each with or without silane pretreatment. Bonded specimens were stored in water for 24 h. The shear bond strength (SBS) of the ceramic-resin interface was measured to evaluate bond strength, and the debonded interface after the SBS test was analysed using field-emission scanning electron microscopy to determine failure mode. Light microscopy was performed to analyse microleakage and marginal sealing ability. Silane pretreatment significantly and positively influenced SBS and marginal sealing ability. For all the universal adhesive groups, SBS increased and the percentage of microleakage decreased after the pretreatment. Without the pretreatment, SBS and the percentage of microleakage were not significantly different between the silane-containing universal adhesive groups and the silane-free groups. Cohesive failure was the main fracture pattern. The results suggest that additional silane pretreatment can effectively improve the bonding strength and marginal sealing of adhesives to lithium disilicate glass ceramics. The bonding performance of silane-containing universal adhesives without pretreatment is similar to that of silane-free adhesives.
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http://dx.doi.org/10.1111/eos.12340DOI Listing
April 2017

Pannexin3 inhibits TNF-α-induced inflammatory response by suppressing NF-κB signalling pathway in human dental pulp cells.

J Cell Mol Med 2017 03 29;21(3):444-455. Epub 2016 Sep 29.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.

Human dental pulp cells (HDPCs) play a crucial role in dental pulp inflammation. Pannexin 3 (Panx3), a member of Panxs (Pannexins), has been recently found to be involved in inflammation. However, the mechanism of Panx3 in human dental pulp inflammation remains unclear. In this study, the role of Panx3 in inflammatory response was firstly explored, and its potential mechanism was proposed. Immunohistochemical staining showed that Panx3 levels were diminished in inflamed human and rat dental pulp tissues. In vitro, Panx3 expression was significantly down-regulated in HDPCs following a TNF-α challenge in a concentration-dependent way, which reached the lowest level at 10 ng/ml of TNF-α. Such decrease could be reversed by MG132, a proteasome inhibitor. Unlike MG132, BAY 11-7082, a NF-κB inhibitor, even reinforced the inhibitory effect of TNF-α. Quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to investigate the role of Panx3 in inflammatory response of HDPCs. TNF-α-induced pro-inflammatory cytokines, interleukin (IL)-1β and IL-6, were significantly lessened when Panx3 was overexpressed in HDPCs. Conversely, Panx3 knockdown exacerbated the expression of pro-inflammatory cytokines. Moreover, Western blot, dual-luciferase reporter assay, immunofluorescence staining, qRT-PCR and ELISA results showed that Panx3 participated in dental pulp inflammation in a NF-κB-dependent manner. These findings suggested that Panx3 has a defensive role in dental pulp inflammation, serving as a potential target to be exploited for the intervention of human dental pulp inflammation.
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http://dx.doi.org/10.1111/jcmm.12988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323855PMC
March 2017
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