Publications by authors named "Hongyan Liu"

430 Publications

Humanized CD19-Targeted Chimeric Antigen Receptor (CAR) T Cells in CAR-Naive and CAR-Exposed Children and Young Adults With Relapsed or Refractory Acute Lymphoblastic Leukemia.

J Clin Oncol 2021 Jun 22:JCO2003458. Epub 2021 Jun 22.

Division of Oncology, Children's Hospital of Philadelphia, Philadelphia, PA.

Purpose: CD19-targeted chimeric antigen receptor (CAR)-modified T cells demonstrate unprecedented responses in B-cell acute lymphoblastic leukemia (B-ALL); however, relapse remains a substantial challenge. Short CAR T-cell persistence contributes to this risk; therefore, strategies to improve persistence are needed.

Methods: We conducted a pilot clinical trial of a humanized CD19 CAR T-cell product (huCART19) in children and young adults with relapsed or refractory B-ALL (n = 72) or B-lymphoblastic lymphoma (n = 2), treated in two cohorts: with (retreatment, n = 33) or without (CAR-naive, n = 41) prior CAR exposure. Patients were monitored for toxicity, response, and persistence of huCART19.

Results: Seventy-four patients 1-29 years of age received huCART19. Cytokine release syndrome developed in 62 (84%) patients and was grade 4 in five (6.8%). Neurologic toxicities were reported in 29 (39%), three (4%) grade 3 or 4, and fully resolved in all cases. The overall response rate at 1 month after infusion was 98% (100% in B-ALL) in the CAR-naive cohort and 64% in the retreatment cohort. At 6 months, the probability of losing huCART19 persistence was 27% (95% CI, 14 to 41) for CAR-naive and 48% (95% CI, 30 to 64) for retreatment patients, whereas the incidence of B-cell recovery was 15% (95% CI, 6 to 28) and 58% (95% CI, 33 to 77), respectively. Relapse-free survival at 12 and 24 months, respectively, was 84% (95% CI, 72 to 97) and 74% (95% CI, 60 to 90) in CAR-naive and 74% (95% CI, 56 to 97) and 58% (95% CI, 37 to 90) in retreatment cohorts.

Conclusion: HuCART19 achieved durable remissions with long-term persistence in children and young adults with relapsed or refractory B-ALL, including after failure of prior CAR T-cell therapy.
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http://dx.doi.org/10.1200/JCO.20.03458DOI Listing
June 2021

Spatial-temporal analysis of China's carbon intensity: a ST-IDA decomposition based on energy input-output table.

Environ Sci Pollut Res Int 2021 Jun 21. Epub 2021 Jun 21.

Department of Economic Management, North China Electric Power University, Baoding, 071000, China.

It is of crucial importance to identify the driving factors for emission changes since China's commitment to reduce carbon intensity in 2009. Hence, the spatial-temporal variation of carbon intensity of China's 30 provinces from 2010 to 2017 is explored by applying a Spatial-temporal Index decomposition analysis (ST-IDA) model combined with energy input-output analysis. Industrial structure, energy intensity, energy structure, and carbon emission coefficient are identified as driving factors; simultaneously, a new factor, energy conversion efficiency, is also introduced based on the energy input-output analysis, which is of significance as China is vigourously pushing electricitification. The results show that the carbon intensity of economic sectors in most provinces declined from 2010 to 2017. Energy intensity is the biggest contributor to both the temporal decline of carbon intensity and its spatial difference for economic sectors, followed by industrial structure, energy conversion efficiency, energy structure and carbon emission coefficient, while the rank of inhibition of each factor is the same as above. Meanwhile, the carbon intensity of the residential sector is mainly affected by per capita GDP and per capita energy consumption. Related policy suggestions are given.
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http://dx.doi.org/10.1007/s11356-021-14877-4DOI Listing
June 2021

Exploring the toxic effects and mechanism of methoxylated polybrominated diphenyl ethers (MeO-PBDEs) on thyroxine-binding globulin (TBG): Synergy between spectroscopic and computations.

Luminescence 2021 Jun 9. Epub 2021 Jun 9.

College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, China.

The interaction mechanism between thyroxine-binding globulin (TBG) and three methoxylated polybrominated diphenyl ethers (MeO-PBDEs) was analyzed by steady-state fluorescence, ultraviolet-visible (UV-vis) spectroscopy, circular dichroism (CD), molecular docking and molecular dynamics simulation methods. The results of molecular docking technique revealed that 2'-MeO-BDE-3, 5-MeO-BDE-47, and 3-MeO-BDE-100 combined with TBG at the active site. The steady-state fluorescence spectra displayed that MeO-PBDEs quenched the endogenous fluorescence of TBG through static quenching mechanism, and complex formation between MeO-PBDEs and TBG was further indicated by UV-vis spectroscopy. The thermodynamic quantities showed that the binding process is spontaneous, and the major forces responsible for the binding are hydrogen bonding and hydrophobic interactions, which are consistent with the results of molecular docking to a certain extent. The results of CD confirmed that the secondary structure of TBG was changed after combining with MeO-PBDEs. The dynamic simulation results illustrated that the protein structure is more compact and changes in the secondary structure of TBG after binding to MeO-PBDEs. Additionally, we also utilized the MM-PBSA method to analyze the binding free energy of TBG and MeO-PBDEs. The results suggest that van der Waals force plays an essential role in the combination.
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http://dx.doi.org/10.1002/bio.4103DOI Listing
June 2021

Gibberellin Increases the Bud Yield and Theanine Accumulation in (L.) Kuntze.

Molecules 2021 May 29;26(11). Epub 2021 May 29.

Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410125, China.

Tea () is one of the most important cash crops in the world. Theanine, as an important amino acid component in tea, is a key quality index for excellent tea quality and high economic value. People increase theanine accumulation in tea mainly through the application of nitrogen fertilizer, shading and pruning. However, these methods are not effective. In this study, we treated tea buds with a 100 μM solution of GA containing 1‰ tween-20, investigated the effects of GA on theanine accumulation, bud yield, chlorophyll fluorescence parameters and expression level of theanine biosynthesis pathway genes in tea plant by qPCR, LC-MS/MS etc. Results showed that change trends of theanine and GA was extremely positively correlated with each other. Exogenous GA upregulated the expression level of theanine biosynthesis pathway genes, caused an increase of theanine content (mg·g) by 27% in tea leaves compared with Mock, and accelerated the germination of buds and elongation of shoots, which lead to a significant increase of tea yield by 56% (/). Moreover, the decrease of chlorophyll contents, photochemical quenching coefficient () and relative electron transport rate () under GA treatment suggested that GA reduced photosynthesis in the tender tea leaves, indicating that the decline of carbon assimilation in tea plants was conducive to the nitrogen metabolism, and it was beneficial to the accumulation of theanine. This study provided a new technical and theoretical support for the precise control of tea quality components and phenophase.
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http://dx.doi.org/10.3390/molecules26113290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198828PMC
May 2021

mir-126-5p Promotes Cisplatin Sensitivity of Non-Small-Cell Lung Cancer by Inhibiting ADAM9.

Biomed Res Int 2021 15;2021:6622342. Epub 2021 May 15.

Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan 430060, China.

Objective: The aim of the study was to investigate molecular mechanisms underlying the role of miR-126-5p in cisplatin (DDP) sensitivity of non-small-cell lung cancer (NSCLC).

Methods: The expression of miR-126-5p and ADAM9 in NSCLC cancer tissues and adjacent tissues, cisplatin-sensitive and drug-resistant NSCLC patient tissues, human normal lung epithelial cells (BESA-2B), human lung adenocarcinoma cell lines A549 and H1560, and cisplatin-resistant mutant cell lines A549/DDP and H1560/DDP was detected by qRT-PCR. After overexpression of miR-126-5p or ADAM9 in A549/DDP and H1560/DDP, MTT and clone formation were used to detect the cell proliferation ability of each treatment group. Flow cytometry was used to detect changes in cell apoptosis. The protein expression of ADAM9 and key molecules of PTEN/PI3K/Akt pathways in cells was measured by western blot.

Results: Compared with NSCLC adjacent tissues and NSCLC cisplatin-sensitive tissues, miR-126-5p expression was downregulated in NSCLC tissues and cisplatin-resistant NSCLC tissues and ADAM9 was upregulated. qRT-PCR further detected that miR-126-5p was downregulated in A549, H1560, and their cisplatin-resistant strains A549/DDP and H1560/DDP, while ADAM9 was upregulated. Moreover, overexpression of miR-126-5p inhibited A549/DDP and H1560/DDP cell proliferation and promoted cell apoptosis. The results of dual luciferase showed that miR-126-5p targeted and negatively regulated ADAM9. We also found that overexpression of ADAM9 could reverse the effects of miR-126-5p on NSCLC cell proliferation, apoptosis, and cisplatin sensitivity, and this effect may be achieved by inhibiting the activity of the PTEN/PI3K/Akt signaling pathway.

Conclusion: Our data indicated that miR-126-5p may negatively regulate ADAM9 to promote the sensitivity of clinical DDP treatment of NSCLC and be a potential therapeutic target for NSCLC treatment.
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http://dx.doi.org/10.1155/2021/6622342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147552PMC
May 2021

BKM120 sensitizes glioblastoma to the PARP inhibitor rucaparib by suppressing homologous recombination repair.

Cell Death Dis 2021 May 26;12(6):546. Epub 2021 May 26.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, China.

PARP inhibitors have been approved for the therapy of cancers with homologous recombination (HR) deficiency based on the concept of "synthetic lethality". However, glioblastoma (GBM) patients have gained little benefit from PARP inhibitors due to a lack of BRCA mutations. Herein, we demonstrated that concurrent treatment with the PARP inhibitor rucaparib and the PI3K inhibitor BKM120 showed synergetic anticancer effects on GBM U251 and U87MG cells. Mechanistically, BKM120 decreased expression of HR molecules, including RAD51 and BRCA1/2, and reduced HR repair efficiency in GBM cells, therefore increasing levels of apoptosis induced by rucaparib. Furthermore, we discovered that the two compounds complemented each other in DNA damage response and drug accumulation. Notably, in the zebrafish U87MG-RFP orthotopic xenograft model, nude mouse U87MG subcutaneous xenograft model and U87MG-Luc orthotopic xenograft model, combination showed obviously increased antitumor efficacy compared to each monotherapy. Immunohistochemical analysis of tumor tissues indicated that the combination obviously reduced expression of HR repair molecules and increased the DNA damage biomarker γ-H2AX, consistent with the in vitro results. Collectively, our findings provide new insight into combined blockade of PI3K and PARP, which might represent a promising therapeutic approach for GBM.
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http://dx.doi.org/10.1038/s41419-021-03805-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150626PMC
May 2021

Comparison of the efficacy of laser-activated and ultrasonic-activated techniques for the removal of tricalcium silicate-based sealers and gutta-percha in root canal retreatment: a microtomography and scanning electron microscopy study.

BMC Oral Health 2021 05 22;21(1):275. Epub 2021 May 22.

Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong Province, China.

Background: Tricalcium silicate-based sealers have been usually indicated for the single-cone technique and result in more residual filling materials in root canal retreatment. Passive ultrasonic irrigation and photon-initiated photoacoustic streaming have been reported to improve the removal efficacy of root canal filling materials. However, the abilities of both techniques combined with NiTi re-instrumentation to remove residual tricalcium silicate-based sealer and gutta-percha have not been compared. The aim of this study was to evaluate the efficacy of laser-activated and ultrasonic-activated techniques in vitro for the removal of the tricalcium silicate-based sealer iRoot SP and gutta-percha after standard canal retreatment procedures with the use of nickel-titanium (NiTi) rotary instruments.
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http://dx.doi.org/10.1186/s12903-021-01638-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141189PMC
May 2021

A study of breastfeeding practices, SARS-CoV-2 and its antibodies in the breast milk of mothers confirmed with COVID-19.

Lancet Reg Health West Pac 2020 Nov 10;4:100045. Epub 2020 Nov 10.

Department of Neonatology, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei 430070, China.

Background: The possibility of 2019 novel coronavirus disease (COVID-19) transmission to neonates through breast milk remains unverified.

Methods: This paper presents the interim results of a longitudinal study being carried out in Hubei province. As of 1 April 2020, 24 mothers confirmed with COVID-19, 19 mothers suspected with COVID-19 but Polymerase chain reaction negative, and 21 mothers without COVID-19 and their neonates have been recruited. Telephone follow-up was conducted to collect information on breastfeeding practices. Forty-four breast milk samples were collected from 16 of the 24 mothers with confirmed COVID-19 for the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) ribonucleic acid (RNA) and antibodies (IgM and IgG) testing.

Findings: The average mother-child separation time was 36•7 ± 21•1 days among mothers confirmed with COVID-19, significantly longer than that of the suspected group (16•6 ± 13•1 days) and control group (10•5 ± 8•2 days). Both the COVID-19 confirmed (58•3%) and suspected (52•6%) groups presented significantly lower rates of breastfeeding as compared with the control group (95•2%). All 44 breast milk samples tested negative for the SARS-CoV-2 nucleic acid. Thirty-eight breast milk samples underwent antibody testing and all tested negative for IgG. Twenty-one breast milk samples from 8 women tested positive for IgM, while the remaining samples from 11 women tested negative.

Interpretation: Considering the lack of evidence for SARS-CoV-2 transmission through breast milk, breastfeeding counselling along with appropriate hand hygiene precautions and facemasks should be provided to all pregnant women.

Funding: The study was funded by the Hong Kong Committee for UNICEF.
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http://dx.doi.org/10.1016/j.lanwpc.2020.100045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7654387PMC
November 2020

Application Value of Lipoprotein-Associated Phospholipase A2 Expression Level in Evaluating the Risk of Major Bleeding in Gestational Diabetes.

Clin Lab 2021 May;67(5)

Background: Studies have shown that obesity and lipid metabolism disorders can lead to increased Lp-PLA2 ac-tivity in the body. However, few studies have reported the correlation between Lp-PLA2 and postpartum hemorrhage in pregnant women with GMD.

Methods: Clinical data was collected from 43 pregnant women with gestational diabetes combined with postpartum hemorrhage and 50 pregnant women with postpartum diabetes without postpartum bleeding during the same period. Lp-PLA2 expression levels in the serum of pregnant women in both groups were detected. The predictive value of Lp-PLA2 expression level for postpartum hemorrhage was clarified.

Results: (1) From 20w to 32w pregnancy, the serum Lp-PLA2 levels of pregnant women in both groups showed a gradual increasing trend. (2) The serum Lp-PLA2 level of the pregnant women in the research group was significantly higher (p < 0.001). (3) Binary logistic regression analysis shows that Lp-PLA2 level has a good correlation with postpartum major bleeding. (4) Comparing of Lp-PLA2 levels in different gestational weeks with respect to its efficacy for predicting postpartum hemorrhage found that the sensitivity and specificity of 24w Lp-PLA2 levels are higher than other gestational weeks, and the area under the 24w parameter curve (0.955) > 32w area under the parameter curve (0.952) > area under the 20w parameter curve (0.940) > area under the 28w parameter curve (0.887). (5) The birth weight of the newborn in the research group was significantly heavier (p < 0.001).

Conclusions: Dynamic monitoring of the expression level of Lp-PLA2 in the serum of pregnant women can predict the risk of postpartum hemorrhage in pregnant women at an early stage and help optimize the rescue plan for postpartum hemorrhage.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200906DOI Listing
May 2021

Association Between Serum Irisin and Diabetic Nephropathy in Patients with Type 2 Diabetes Mellitus: A Meta-Analysis.

Horm Metab Res 2021 May 7;53(5):293-300. Epub 2021 May 7.

Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, China.

Irisin, an emerging adipokine, has been involved in the pathogenesis of type 2 diabetes mellitus (T2DM). However, previous studies evaluating the association between irisin and diabetic nephropathy (DN) showed inconsistent results. We performed a meta-analysis to evaluate the above association. Matched case-control studies evaluating the difference of serum irisin between T2DM patients with and without DN were identified via systematic search of PubMed, Embase, Cochranes' Library, China National Knowledge Infrastructure, and WanFang databases from inception to December 5, 2020. A random-effects model or a fixed-effects model was used to pool the results according to the heterogeneity. Overall, thirteen matched case-control studies including 1735 T2DM patients were included. Results of meta-analysis showed that compared to T2DM patients with normoalbuminuria, those with microalbuminuria [10 studies, standard mean difference (SMD): 1.12, 95% confidence interval (CI): 0.48-1.77, p<0.001; I=94%] and macroalbuminuria (10 studies, SMD: 1.86, 95% CI: 0.93-2.79, p<0.001; I=97%) had significantly lower serum irisin. Besides, the serum level of irisin was significantly lower in T2DM patients with macroalbuminuria than those with microalbuminuria (10 studies, SMD: 0.91, 95% CI: 0.44-1.38, p<0.001; I=90%). In addition, patients with estimated glomerular infiltration rate (eGFR)<60 ml/min 1.73 m had lower serum irisin compared to those with eGFR≥60 ml/min 1.73 m (4 studies, SMD: 0.89, 95% CI: 0.32-1.46, p=0.002; I=91%). In conclusion, serum irisin may be associated with albuminuria and reduced eGFR in T2DM patients.
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http://dx.doi.org/10.1055/a-1475-4444DOI Listing
May 2021

Tyrosine Kinase Inhibitor Cabozantinib Inhibits Murine Renal Cancer by Activating Innate and Adaptive Immunity.

Front Oncol 2021 19;11:663517. Epub 2021 Apr 19.

Cancer Institute, Xuzhou Medical University, Xuzhou, China.

Background: Advanced renal cell carcinoma (RCC) has a very dismal prognosis. Cabozantinib, a tyrosine kinase inhibitor, has been approved for the treatment of advanced RCC. However, the impact of cabozantinib on the immune microenvironment of RCC remains poorly understood.

Methods: Kaplan-Meier survival curves were constructed to examine the correlation between intratumor infiltration of neutrophils and patient prognosis in RCC. Infiltration and effector function of neutrophils and T cells in response to cabozantinib treatment were investigated in a murine RCC model.

Results: A retrospective study of 307 RCC patients indicated that neutrophils were recruited into tumor tissues, and increased neutrophil infiltration was associated with improved clinical outcomes. In a murine model of RCC, cabozantinib treatment significantly increased both intratumor infiltration and anti-tumor function of neutrophils and T cells. Mechanistically, we found that cabozantinib treatment induced expression of neutrophil-related chemokines (CCL11 and CXCL12) and T cell-related chemokines (CCL8 and CX3CL1) in the tumor microenvironment. Furthermore, depletion of neutrophils and CD8 T cells compromised the therapeutic efficacy of cabozantinib. Importantly, cabozantinib treatment induced long-term anti-tumor T cell response.

Conclusions: Our study revealed novel mechanisms of the therapeutic effects of cabozantinib on RCC by activating both neutrophil-mediated innate immunity and T cell-mediated adaptive immunity. These findings are of great significance for guiding the clinical use of cabozantinib and provide a good candidate for future combination therapy with T-cell therapies or other immunotherapies.
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http://dx.doi.org/10.3389/fonc.2021.663517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8089383PMC
April 2021

Fast and accurate modeling of transient-state, gradient-spoiled sequences by recurrent neural networks.

NMR Biomed 2021 Jul 5;34(7):e4527. Epub 2021 May 5.

Center for Image Sciences, University Medical Center Utrecht, Utrecht, the Netherlands.

Fast and accurate modeling of MR signal responses are typically required for various quantitative MRI applications, such as MR fingerprinting. This work uses a new extended phase graph (EPG)-Bloch model for accurate simulation of transient-state, gradient-spoiled MR sequences, and proposes a recurrent neural network (RNN) as a fast surrogate of the EPG-Bloch model for computing large-scale MR signals and derivatives. The computational efficiency of the RNN model is demonstrated by comparisons with other existing models, showing one to three orders of acceleration compared with the latest GPU-accelerated, open-source EPG package. By using numerical and in vivo brain data, two used cases, namely, MRF dictionary generation and optimal experimental design, are also provided. Results show that the RNN surrogate model can be efficiently used for computing large-scale dictionaries of transient-state signals and derivatives within tens of seconds, resulting in several orders of magnitude acceleration with respect to state-of-the-art implementations. The practical application of transient-state quantitative techniques can therefore be substantially facilitated.
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http://dx.doi.org/10.1002/nbm.4527DOI Listing
July 2021

Neurodevelopmental outcomes in mild and moderate isolated ventriculomegaly originating .

J Matern Fetal Neonatal Med 2021 May 4:1-8. Epub 2021 May 4.

Department of Pediatrics, Peking University First Hospital, Beijing, China.

Objective: To determine the differences in outcomes between mild and moderate isolated ventriculomegaly (IVM).

Methods: We conducted a prospective cohort study on 94 fetuses with IVM and evaluated the neurodevelopmental outcomes at 12 months of age using the ASQ-3 and BSID-I neurodevelopmental assessment tools. Progression of VM was defined as an increase in the width of the ventricular by at least 3 mm during sequential ultrasound monitoring. The population was divided into two groups according to ventricular width: mild (10-12 mm) and moderate (12.1-15 mm), which were further evaluated for VM progression in utero separately.

Results: Neurodevelopmental assessments at 12 months were the main form of evaluations. Neurodevelopmental impairment (NDI) was defined as a mental development index (MDI) or psychomotor development index (PDI) < 85. There were no significant differences in NDI values between the mild and moderate groups ( = .155). Compared with the non- progression group (7.6%), the rate of NDI was significantly higher ( = .004) in the group with progression (33.3%). Using linear regression and correlation, no negative correlation was found between the maximum value of atrial diameter (AD) in utero and the PDI ( = -0.021,  = .914) or MDI ( = -0.073,  = .703) score. However, the maximum change in the AD in utero was negatively correlated with both PDI ( = -0.460,  = .011) and MDI (=-0.422,  = .020) scores.

Conclusion: There were likely no differences in neurodevelopmental outcomes between mild and moderate IVM. In fetuses with mild to moderate VM, intrauterine progression may be a poor prognostic factor for neurodevelopmental outcomes.
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http://dx.doi.org/10.1080/14767058.2021.1919869DOI Listing
May 2021

The effect of introducing Ga on the ZSM-5-catalyzed methanol to aromatics reaction: taking methylcyclopentane to benzene as an example.

Phys Chem Chem Phys 2021 May 30;23(18):10988-11003. Epub 2021 Apr 30.

Key Laboratory of Coal Science and Technology of Ministry of Education and Shanxi Province, Taiyuan University of Technology, No. 79 Yingze West Street, Taiyuan 030024, Shanxi, P. R. China.

Naphthenes are key intermediates in the formation of aromatic compounds during the methanol to aromatics (MTA) reaction, and the dehydrogenation process is more important than the hydrogen transfer process. Theoretical studies were performed to investigate the methylcyclopentane, which represents a naphthene, to benzene MTA process catalyzed by ZSM-5 before and after introducing Ga, showing that Ga-ZSM-5 was more favorable for carrying out the reaction than two H-type ZSM-5 (H-Z and H-Z) models. H-Z and H-Z are favorable for the transfer of H during ring expansion reactions and the reformation of Brønsted acids, but the dehydrogenation reactions involving H-Z and H-Z require high free-energy barriers to be overcome. Although introducing Ga to ZSM-5 is not conducive to the transfer of H after dehydrogenation, it can reduce the extremely high dehydrogenation free-energy barrier compared with H-Z and H-Z; this is mainly because Ga at dehydrogenation active centers, [GaH], can accept electrons and donate them to the H atoms of [GaH], giving H negative charge and making it easy to combine with positive B-acid H atoms that come from methylcyclopentane, cyclohexene, and cyclohexadiene to produce H. Also, analysis of the transition state structures of all DH processes shows that Ga-ZSM-5 is more favorable for promoting the combination of H to produce H than H-Z and H-Z.
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http://dx.doi.org/10.1039/d0cp05778aDOI Listing
May 2021

An intracellular nanobody targeting T4SS effector inhibits infection.

Proc Natl Acad Sci U S A 2021 May;118(18)

Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210;

Infection with obligatory intracellular bacteria is difficult to treat, as intracellular targets and delivery methods of therapeutics are not well known. translocated factor-1 (Etf-1), a type IV secretion system (T4SS) effector, is a primary virulence factor for an obligatory intracellular bacterium, In this study, we developed Etf-1-specific nanobodies (Nbs) by immunizing a llama to determine if intracellular Nbs block Etf-1 functions and infection. Of 24 distinct anti-Etf-1 Nbs, NbD7 blocked mitochondrial localization of Etf-1-GFP in cotransfected cells. NbD7 and control Nb (NbD3) bound to different regions of Etf-1. Size-exclusion chromatography showed that the NbD7 and Etf-1 complex was more stable than the NbD3 and Etf-1 complex. Intracellular expression of NbD7 inhibited three activities of Etf-1 and : up-regulation of mitochondrial manganese superoxide dismutase, reduction of intracellular reactive oxygen species, and inhibition of cellular apoptosis. Consequently, intracellular NbD7 inhibited infection, whereas NbD3 did not. To safely and effectively deliver Nbs into the host cell cytoplasm, NbD7 was conjugated to cyclized cell-permeable peptide 12 (CPP12-NbD7). CPP12-NbD7 effectively entered mammalian cells and abrogated the blockade of cellular apoptosis caused by and inhibited infection by in cell culture and in a severe combined-immunodeficiency mouse model. Our results demonstrate the development of an Nb that interferes with T4SS effector functions and intracellular pathogen infection, along with an intracellular delivery method for this Nb. This strategy should overcome current barriers to advance mechanistic research and develop therapies complementary or alternative to the current broad-spectrum antibiotic.
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http://dx.doi.org/10.1073/pnas.2024102118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106314PMC
May 2021

Implementation of a Multidisciplinary Debriefing Process for Pediatric Ward Deterioration Events.

Hosp Pediatr 2021 May 15;11(5):454-461. Epub 2021 Apr 15.

Anesthesiology and Critical Care Medicine.

Objectives: Event debriefing has established benefit, but its adoption is poorly characterized among pediatric ward providers. To improve patient safety, our hospital restructured its debriefing process for ward deterioration events culminating in ICU transfer. The aim of this study was to describe this process' implementation.

Methods: In the restructured process, multidisciplinary ward providers are expected to debrief all ICU transfers. We conducted a multimethod analysis using facilitative guides completed by debriefing participants. Monthly debriefing completion served as an adoption metric.

Results: Between March 2019 and February 2020, providers across 9 wards performed debriefing for 134 of 312 PICU transfers (43%). Bedside nurses participated most frequently (117 debriefings [87%]). There was no significant difference in debriefing by unit, acuity, season, or nurse staffing. Compared with units fully staffed by rotational frontline clinicians (FLCs; eg, resident physicians), units with dedicated FLCs whose responsibilities are primarily limited to that unit (eg, oncology hospitalists) completed significantly more monthly debriefings (average [SD] 57% [30%] vs 33% [28%] of PICU transfers; = .004). FLC participation was also higher on these units (50% of debriefings [37%] vs 24% [37%]; = .014). Through qualitative analysis, we identified distinct debriefing themes, with teaming activities such as communication cited most often.

Conclusions: Implementation of a multidisciplinary debriefing process for ward deterioration events culminating in ICU transfer was associated with differential adoption across providers and FLC staffing models but not acuity or nurse staffing. Teaming activities were a debriefing priority. Future study will assess patient safety outcomes.
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http://dx.doi.org/10.1542/hpeds.2020-002014DOI Listing
May 2021

A multi-center survey on the postpartum mental health of mothers and attachment to their neonates during COVID-19 in Hubei Province of China.

Ann Transl Med 2021 Mar;9(5):382

Health, Nutrition and WASH, UNICEF China, Beijing, China.

Background: There is an emerging literature on the mental health of both pre- and post-partum mothers during the coronavirus disease 2019 (COVID-19) pandemic.

Methods: As of April 1, 2020, 23 mothers confirmed with COVID-19, 15 mothers suspected with COVID-19 but with negative polymerase chain reaction tests, and 33 mothers without COVID-19 (Control Group) were recruited for a study from Hubei Province in China. The Maternal Postnatal Attachment Scale (MPAS), the Zung Self-rating Anxiety Scale, and the Zung Self-rating Depression Scale were applied to investigate the attachment of mothers to their neonates and the postpartum mental health of mothers within the first 3 months after delivery (between 20 to 89 days).

Results: The period of mother-child separation among the confirmed group (33.9±20.9 days) was significantly longer than that of suspected group (16.7±12.2 days) and control group (10.7±8.4 days). The total score of the MPAS in mothers confirmed with COVID-19 (45.5±4.2) was significantly lower (indicating less mother-child attachment) than that in the suspected (50.5±4.7) and control (48.8±4.6) groups. A negative correlation was noted between the mother-child separation time and the MPAS scores, including the subscale scores of attachment (MPAS acore: Spearman's ρ =-0.33, 95% CI: -0.095 to -0.538, P=0.005; Subscale score of attachment: Spearman's ρ =-0.40, 95% CI: -0.163 to -0.592, P=0.001). The incidence of postpartum anxiety in the confirmed, suspected and control groups was 4.3%, 6.7% and 12.1%, respectively; and the incidence of postpartum depression was 39.1%, 33.3% and 30.3%, respectively. No significant difference was found with regards to maternal postpartum anxiety and depression among the three groups.

Conclusions: Decreased mother-child attachment found among mothers confirmed with COVID-19, indicates that further intervention is needed to ensure mother-child interaction to appropriately develop attachment. Mother-child attachment experienced disruption due to prolonged mother-child separation necessitated by the COVID-19 management protocol, which needs to be revised to reduce prolonged mother-child separation. Additionally, mothers with and without COVID-19 suffered a high incidence of depression, which warrants further mental health investment for pregnant mothers during the COVID-19 pandemic.
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http://dx.doi.org/10.21037/atm-20-6115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033344PMC
March 2021

Optimizing the SEM specimen preparation method for accurate microanalysis of carbon nanotube/nanocluster hybrids.

J Microsc 2021 Jun 14;282(3):267-273. Epub 2021 Apr 14.

State Key Laboratory of Automotive Simulation and Control, and School of Materials Science & Engineering, and Electron Microscopy Center, and International Center of Future Science, Jilin University, Changchun, China.

Scanning electron microscopy (SEM) integrated with energy-dispersive X-ray spectrometry (EDS) are scientifically used to characterise the morphology, chemical composition and elemental distribution of powder samples. Upon an accessible analytical instrument, the specimen preparation method directly affects the quality and accuracy of the observation and analysis. In this paper, three preparation methods were utilised to characterise the carbon nanotubes (CNTs) based materials, and their strengths as well as the limitations are discussed. Thus, a characterisation strategy was established by comparing the obtained three measurement together with the derived sample information. To the end, we proposed to acquire the backscattered electron (BSE) images of nanoscale heavier nanoclusters grafted CNTs, typically for wide functional applications such as energy conversion and storage. Our proposed optimum method works particularly on the clarification of powder samples with small particle sizes and low atomic numbers, which underscores the involved contribution of SEM backscattered electron images.
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http://dx.doi.org/10.1111/jmi.13008DOI Listing
June 2021

The mechanism of sesame resistance against Macrophomina phaseolina was revealed via a comparison of transcriptomes of resistant and susceptible sesame genotypes.

BMC Plant Biol 2021 Mar 29;21(1):159. Epub 2021 Mar 29.

School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.

Background: Sesame (Sesamum indicum) charcoal rot, a destructive fungal disease caused by Macrophomina phaseolina (Tassi) Goid (MP), is a great threat to the yield and quality of sesame. However, there is a lack of information about the gene-for-gene relationship between sesame and MP, and the molecular mechanism behind the interaction is not yet clear. The aim of this study was to interpret the molecular mechanism of sesame resistance against MP in disease-resistant (DR) and disease-susceptible (DS) genotypes based on transcriptomics. This is the first report of the interaction between sesame and MP using this method.

Results: A set of core genes that response to MP were revealed by comparative transcriptomics and they were preferentially associated with GO terms such as ribosome-related processes, fruit ripening and regulation of jasmonic acid mediated signalling pathway. It is also exhibited that translational mechanism and transcriptional mechanism could co-activate in DR so that it can initiate the immunity to MP more rapidly. According to weighted gene co-expression network analysis (WGCNA) of differentially expressed gene sets between two genotypes, we found that leucine-rich repeat receptor-like kinase (LRR-RLK) proteins may assume an important job in sesame resistance against MP. Notably, compared with DS, most key genes were induced in DR such as pattern recognition receptors (PRRs) and resistance genes, indicating that DR initiated stronger pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). Finally, the study showed that JA/ET and SA signalling pathways all play an important role in sesame resistance to MP.

Conclusions: The defence response to MP of sesame, a complex bioprocess involving many phytohormones and disease resistance-related genes, was illustrated at the transcriptional level in our investigation. The findings shed more light on further understanding of different responses to MP in resistant and susceptible sesame.
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http://dx.doi.org/10.1186/s12870-021-02927-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008628PMC
March 2021

Discrimination of goat, buffalo, and yak milk from different livestock, regions, and lactation using microelement contents.

J Food Sci 2021 Apr 24;86(4):1283-1295. Epub 2021 Mar 24.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.

This study aims at investigating whether the livestock, lactation stage, or geographical origin of specialty milk in China could be classified by the elemental contents. Samples of goat, buffalo, and yak milk were collected from main production provinces in China and the contents of nine elements in milk from different livestock, regions, and lactation stages were determined. Statistical analysis indicated that the contents of Mn, Cu, As, Se, Cs, and Mo in mature milk were significantly different between the three livestock (p < 0.05). The trends of Mn and Cs contents of the goat milk and buffalo milk increased first and then decreased gradually during lactation. The contents of Se, Cs, Ba, and Mo in mature milk of three livestock were significantly different between the three regions (p < 0.05). The result of linear discriminant analysis (LDA) and partial least squares discrimination analysis (PLS-DA) showed that the total correct classification rates in different livestock for mature milk reached 86.7 and 90%. For goat milk, the total correct classification rates of different lactations and regions reached 88.9 and 77.8% for LDA (100 and 87.5% for PLS-DA), and followed by yak milk of different regions which achieved 88.9% for LDA (88.9% for PLS-DA). In summary, the microelements in milk showed a significant correlation with livestock, region, and lactation of goat, buffalo, and yak milk. The elemental fingerprints combined with the LDA could be potential for classifying the livestock, lactation stage, and geographical origin of milk. PRACTICAL APPLICATION: According to study the contents of miroelements in milk from different livestock, regions, and lactation stages, provide evidence and support for classifying the livestock, lactation stage, and geographical origin of milk.
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http://dx.doi.org/10.1111/1750-3841.15685DOI Listing
April 2021

Finerenone-A New Frontier in Renin-Angiotensin-Aldosterone System Inhibition in Diabetic Kidney Disease.

Am J Kidney Dis 2021 Mar 19. Epub 2021 Mar 19.

Department of Medicine, Division of Nephrology, UHN, Toronto, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada; Department of Physiology, University of Toronto, Toronto, Ontario; Banting and Best Diabetes Centre, Toronto, Canada. Electronic address:

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http://dx.doi.org/10.1053/j.ajkd.2021.02.324DOI Listing
March 2021

Regiodivergent Desymmetrization Reaction of -Azabicycloheptene Providing Two Enantioenriched Structural Isomers.

Org Lett 2021 Apr 22;23(7):2411-2414. Epub 2021 Mar 22.

Department of Chemistry, Graduate School of Science, Kobe University, Nada, Kobe, 657-8501, Japan.

A novel catalytic asymmetric reaction is reported where the regiodivergent desymmetrisation of -azabicycloheptene via allylic oxidation using a single chiral copper catalyst produced two different, enantioenriched structural isomers in high optical purity starting from a single compound. The enantioselectivity of the two structurally isomeric compounds was enriched to >99.5% ee after derivatization.
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http://dx.doi.org/10.1021/acs.orglett.1c00036DOI Listing
April 2021

Old-growth forests show low canopy resilience to droughts at the southern edge of the taiga.

Glob Chang Biol 2021 Jun 27;27(11):2392-2402. Epub 2021 Mar 27.

College of Urban and Environmental Sciences and MOE Laboratory for Earth Surface Processes, Peking University, Beijing, China.

Forest mortality and resilience driven by drought disturbances have attracted tons of attention. However, the acquisition of continuous spatial-temporal data is generally enslaved to the conventional field investigations. In this study, the resilience of semiarid forest was characterized with canopy dynamics from remote sensing observations, combining the variations in canopy greenness and water content. We integrated dense normalized difference vegetation index (NDVI) and normalized difference infrared index (NDII) time series from Landsat datasets, intending to assess the canopy resilience in 24 conifer patches along a climatic aridity gradient at the southern edge of the taiga in northern Mongolia and southern Siberia of Russia. The results exhibited four patterns of coordinated NDVI-NDII variation trends, indicating that the canopy water content of coniferous forests may decrease at first during a drought period, and sustained water loss may, in turn, induce an accompanying reduction in canopy greenness. Meanwhile, the patches with canopy recovery growth after initial declines were considered to have resilience to climate change. We further observed the combined effects of aridity degree and tree age on canopy resilience, and all seven patches with no resilience corresponded to the old-tree group (the oldest trees reached or exceeded the age of 90). The observations indicated that the old-growth forests in semiarid regions were less likely to show canopy resilience, which corresponded to a higher risk of sustained decline.
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http://dx.doi.org/10.1111/gcb.15605DOI Listing
June 2021

Pulmonary surfactant-associated protein B regulates prostaglandin-endoperoxide synthase-2 and inflammation in chronic obstructive pulmonary disease.

Exp Physiol 2021 May 8;106(5):1303-1311. Epub 2021 Apr 8.

Key Laboratory of National Health Commission for the Diagnosis & Treatment of COPD, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China.

New Findings: What is the central question of this study? It is reported that polymorphism of the gene for pulmonary surfactant-associated protein B (SFTPB) is associated with chronic obstructive pulmonary disease (COPD): what are the function and mechanism of action of SFTPB in COPD? What is the main finding and its importance? Under stimulation of the risk factors of COPD, SFTPB expression is decreased, which may be involved in the formation of COPD. The progress of COPD induces an inflammatory response and reduces SFTPB expression. Levels of prostaglandin-endoperoxide synthase-2 (PTGS2) and inflammatory responses are changed by SFTPB, which indicates that SFTPB promotes the progression of COPD by PTGS2 and inflammation.

Abstract: Pulmonary surfactant-associated protein B (SFTPB) is a critical protein for lung homeostasis, and polymorphism of its gene is associated with chronic obstructive pulmonary disease (COPD). However, few studies have so far confirmed the functional involvement of SFTPB in COPD. Serum SFTPB and inflammatory cytokine levels were measured in 54 patients with acute exacerbation of COPD and 29 healthy controls. A549 cells were induced using 10% cigarette smoke extract (CSE) and treated with dexamethasone to investigate the effect of inflammation on SFTPB expression, and the effect of SFTPB overexpression and silencing on inflammatory cytokines was measured using real-time PCR and enzyme-linked immunosorbent assay. SFTPB expression was assessed in mouse lung tissues using immunofluorescence. Serum levels of SFTPB were significantly lower in COPD patients than in controls (P = 0.009). Conversely, levels of interleukin (IL)-6 and prostaglandin-endoperoxide synthase-2 (PTGS2) were increased in COPD patients (IL-6: P = 0.006; PTGS2: P = 0.043). After CSE treatment, SFTPB mRNA and protein levels were significantly decreased compared to controls (mRNA: P = 0.002; protein: P = 0.011), while IL-6, IL-8 and PTGS2 were elevated. Dexamethasone treatment increased SFTPB levels. Following overexpression of SFTPB in A549 cells, mRNA and protein levels of IL-6, IL-8 and PTGS2 were significantly reduced, while gene silencing induced the opposite effect. SFTPB levels were significantly reduced in the lung tissue of a mouse model of COPD compared to controls. Reduced SFTPB levels may induce PTGS2 and inflammatory responses in COPD and SFTPB could be a key protein for evaluation of COPD progression.
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http://dx.doi.org/10.1113/EP089244DOI Listing
May 2021

Targeting FGFR in non-small cell lung cancer: implications from the landscape of clinically actionable aberrations of FGFR kinases.

Cancer Biol Med 2021 Mar 12. Epub 2021 Mar 12.

Department of Oncology, The Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin 150086, China.

Objective: Dysfunction in fibroblast growth factor receptor (FGFR) signaling has been reported in diverse cancer types, including non-small cell lung cancer (NSCLC). The frequency of aberrations in Chinese NSCLC patients is therefore of great clinical significance.

Methods: A total of 10,966 NSCLC patients whose tumor specimen and/or circulating cell-free DNA (cfDNA) underwent hybridization capture-based next-generation sequencing were reviewed. Patients' clinical characteristics and treatment histories were also evaluated.

Results: aberrations, including mutations, fusions, and gene amplifications, were detected in 1.9% (210/10,966) of the population. abnormalities were more frequently observed in lung squamous cell carcinomas (6.8%, 65/954) than lung adenocarcinomas (1.3%, 128/9,596). oncogenic mutations were identified in 19 patients (~0.17%), of which, 68% were male lung squamous cell carcinoma patients. Eleven out of the 19 patients (58%) had concurrent altered PI3K signaling, thus highlighting a potential combination therapeutic strategy of dual-targeting FGFR and PI3K signaling in such patients. Furthermore, fusions retaining the intact kinase domain were identified in 12 patients (0.11%), including 9 , 1 , 1 novel , and 1 novel fusion between the and 5'-untranslated regions, which may have caused overexpressions. Concomitant mutations or amplifications were observed in 6 patients, and 4 patients received anti-EGFR inhibitors, in whom fusions may have mediated resistance to anti-EGFR therapies. amplification was detected in 24 patients, with the majority being amplifications. Importantly, oncogenic mutations, fusions, and gene amplifications were almost always mutually exclusive events.

Conclusions: We report the prevalence of anomalies in a large NSCLC population, including mutations, gene amplifications, and novel fusions.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0120DOI Listing
March 2021

Relationships between inflammation, hemodynamic function and RAAS in longstanding type 1 diabetes and diabetic kidney disease.

J Diabetes Complications 2021 May 2;35(5):107880. Epub 2021 Feb 2.

Division of Nephrology, Department of Medicine, University of Toronto, Toronto, Ontario, Canada; Banting and Best Diabetes Centre, Toronto, Canada. Electronic address:

The renin angiotensin aldosterone system (RAAS) is associated with renal disease and inflammation in a diabetes setting, however, little is known about the implicated mechanisms in individuals with long standing diabetes. Accordingly, our aim was to perform an observational study to quantify urinary excretion of inflammatory biomarkers in participants with long standing type 1 diabetes (T1D) (with and without diabetic kidney disease [DKD]) and controls, at baseline and in response to RAAS activation. GFR, ERPF, and 42 urine inflammatory biomarkers were measured in 74 participants with T1D for ≥50 years (21 with DKD and 44 without DKD [DKD resistors]) and 73 healthy controls. Additionally, inflammatory biomarkers were measured before and after an angiotensin II infusion (ANGII, 1 ng∙kg∙min). Significantly lower urinary excretion of cytokines (IL-18, IL-1RA, IL-8), chemokines (MCP1, RANTES) and growth factors (TGF-α, PDGFAA, PDGFBB, VEGF-A) was observed in participants with T1D at baseline compared to controls. Urinary IL-6 was higher in DKD than in DKD resistors in an exploratory analysis unadjusted for multiple comparisons. In T1D only, lower GFR correlated with greater excretion of proinflammatory biomarkers (IL-18, IP-10, & RANTES), growth factors (PDGF-AA & VEGFAA), and chemokines (eotaxin & MCP-1). ANGII increased 31 of 42 inflammatory biomarkers in T1D vs controls (p < 0.05), regardless of DKD resistor status. In conclusion, lower GFR and intra-renal RAAS activation were associated with increased inflammation even after longstanding T1D. The increased urinary IL-6 in patients with DKD requires further investigation to determine whether IL-6 is a candidate protective biomarker for prognostication or targeted therapy in DKD.
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http://dx.doi.org/10.1016/j.jdiacomp.2021.107880DOI Listing
May 2021

Transparent TiO/Cu/TiO Multilayer for Electrothermal Application.

Materials (Basel) 2021 Feb 22;14(4). Epub 2021 Feb 22.

Beijing Engineering Research Center of Advanced Structural Transparencies for the Modern Traffic System, Beijing Institute of Aeronautical Materials, Beijing 100095, China.

Highly transparent indium-free multilayers of TiO/Cu/TiO were obtained by means of annealing. The effects of Cu thickness and annealing temperature on the electrical and optical properties were investigated. The critical thickness of Cu mid-layer with optimal electrical and optical properties was 10 nm, with the figure of merit reaching as high as 5 × 10 Ω. Partial crystallization of the TiO layer enhanced the electrical and optical properties upon annealing. Electrothermal experiments showed that temperatures of more than 100 °C can be reached at a heating rate of 2 °C/s without any damage to the multilayers. The experimental results indicate that reliable transparent TiO/Cu/TiO multilayers can be used for electrothermal application.
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http://dx.doi.org/10.3390/ma14041024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926943PMC
February 2021

A preliminary study on pollution characteristics of surfactant substances in fine particles in the Beibu Gulf Region of China.

J Environ Sci (China) 2021 Apr 20;102:363-372. Epub 2020 Oct 20.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100021, China.

The pollution characteristics of surfactant substances in fine particles (PM) in spring were studied in the Beibu Gulf Region of China, 68 samples of PM were collected at Weizhou Island in Beihai City from March 12 to April 17, 2015. The Anionic Surfactant Substances (ASS) and Cationic Surfactant Substances (CSS) in the samples were analyzed using Byethyl Violet Spectrophotometry and Disulfide Blue Spectrophotometry, respectively. Combined with the data from backward trajectory simulation, the effects of air pollutants from remote transport on the pollution characteristics of surfactant substances in PM in the Beibu Gulf Region were analyzed and discussed. The results showed that the daily mean concentrations of ASS and CSS in spring in the Beibu Gulf Region were 165.20 pmol/m and 8.05pmol/m, and the variation ranges were 23.21-452.55 pmol/m and 0.65-31.31 pmol/m, accounting for 1.82‰ ± 1.65‰ and 0.12‰ ± 0.11‰ of the mass concentration of PM, respectively. These concentrations were lower than those in comparable regions around the world. There was no clear correlation between the concentrations of ASS and CSS in PM and the mass concentrations of PM. Tourism and air transport had a positive contribution on the concentrations of ASS. The concentration of surfactant substances in PM was significantly impacted by wind speed and wind direction. Atmospheric temperature, air pressure and precipitation had little effect on the concentrations of surfactant substances. Surfactant substances in PM significantly impacted visibility. Results also showed that the main sources of surfactant substances were from the southern China and Southeast Asia.
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http://dx.doi.org/10.1016/j.jes.2020.08.008DOI Listing
April 2021

Novel pH-Triggered Doxorubicin-Releasing Nanoparticles Self-Assembled by Functionalized β-Cyclodextrin and Amphiphilic Phthalocyanine for Anticancer Therapy.

ACS Appl Mater Interfaces 2021 Mar 23;13(9):10674-10688. Epub 2021 Feb 23.

Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Chemical Engineering College, Qingdao University of Science and Technology, Qingdao 266042, China.

Cyclodextrins (CDs), as pharmaceutical excipients with excellent biocompatibility, non-immunogenicity, and low toxicity in vivo, are widely used to carry drugs by forming inclusion complexes for improving the solubility and stability of drugs. However, the limited space of CDs' lipophilic central cavity affects the loading of many drugs, especially with larger molecules. In this study, β-CDs were modified by acetonization to improve the affinity for the chemotherapy drug doxorubicin (DOX), and doxorubicin-adsorbing acetalated β-CDs (Ac-CD:DOX) self-assembled to nanoparticles, followed by coating with the amphiphilic zinc phthalocyanine photosensitizer ZnPc-(PEG) for antitumor therapy. The final product ZnPc-(PEG):Ac-CD:DOX was demonstrated to have excellent stability and pH-sensitive drug release characteristics. The cell viability and apoptosis assay showed synergistic cytotoxic effects of chemotherapy and phototherapy. The mechanism of cytotoxicity was analyzed in terms of intracellular reactive oxygen species, mitochondrial membrane potential, and subcellular localization. More importantly, in vivo experiments indicated that ZnPc-(PEG):Ac-CD:DOX possessed significant tumor targeting, prominent antitumor activity, and less side effects. Our strategy expands the application of CDs as drug carriers and provides new insights into the development of CD chemistry.
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http://dx.doi.org/10.1021/acsami.0c19027DOI Listing
March 2021

Needs Assessment Survey for a Food Safety Education through We-Media: A Cross-Sectional Survey among Junior Students of an Education and a Medical University in Chongqing, China.

J Nutr Sci Vitaminol (Tokyo) 2020 ;66(Supplement):S267-S274

School of Public Health and Management, Chongqing Medical University.

Many studies on food safety cognition and practice intervention among university students exist, but only few conduct needs assessment surveys. In recent years, We-media has been applied in the field of health education and promotion, but its application in food safety intervention is limited at home and abroad. This study aimed to explore the current situation of We-media use and assess the needs for food safety information through We-media among junior students of an education and a medical university. A cross-sectional survey was conducted among junior students of an education and a medical university in Chongqing, China in 2016. A total of 1,250 education students and 1,434 medical students participated in the questionnaire survey. Findings revealed that 71.4% and 64.8% of the education and medical students were willing to accept food safety educational information by We-media, respectively. In addition, 47.6% and 48.8% of the education and medical students were willing to accept food safety information through WeChat official accounts, respectively. Among the education students, 83.8%, 63.9%, 59.6%, and 13.0% wanted to acquire food safety knowledge by picture, text, video, and voice, respectively. Of the medical students, 84.7%, 67.7%, 62.3%, and 11.9% wanted to acquire food safety knowledge by picture, text, video, and voice, respectively. Gender, school category, and whether food safety information is given attention were the influencing factors of participants' willingness to accept such information through We-media (p<0.05). This study indicated that We-media could be an appropriate intervention approach for the junior students of an education and a medical university to accept food safety intervention. WeChat was also revealed as the best platform. Pictures, text messages, and videos were observed the most popular means for students to acquire food safety knowledge.
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http://dx.doi.org/10.3177/jnsv.66.S267DOI Listing
January 2020