Publications by authors named "Hongwei Zhang"

821 Publications

Research on Family Violence in Greater China: Opportunities, Challenges, and Development.

J Fam Violence 2021 Jun 15:1-5. Epub 2021 Jun 15.

University of Macau, Avenida da Universidade, Taipa, Macau China.

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http://dx.doi.org/10.1007/s10896-021-00295-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204608PMC
June 2021

Exploring the eco-efficiency of cultivated land utilization and its influencing factors in China's Yangtze River Economic Belt, 2001-2018.

J Environ Manage 2021 Jun 8;294:112939. Epub 2021 Jun 8.

School of Public Administration, China University of Geosciences, Wuhan, 430074, China. Electronic address:

Rapid urbanization in China has worsened the sustainable utilization of limited cultivated land resources, which seriously threatens food security and ecological security. To realize maximum benefits and minimize environmental pollution, the eco-efficiency of cultivated land utilization (ECLU) is becoming a vital indicator in weighing the rationality of regional land use. However, conceptualization of the ECLU remains lacking, while assessments of this indicator are still incomplete. This lack of information may inhibit planning guideline for the sustainable development of cultivated land resources. Thus, this study attempts to fill this gap by customizing a new conceptual index system for the ECLU and measuring it using the slack-based measure with undesirable output (SBM-Undesirable) model in the Yangtze River Economic Belt (YREB) during the period 2001-2018. Spatial econometric models were used to further analyze the influencing factors of the ECLU. The average ECLU value in the YREB declined from 2001 to 2004, and then rapidly trended upward in 2005-2018. The lower reaches had the highest efficiency, followed by the middle and upper reaches, with respective values of 0.494, 0.628, and 0.683. The spatial-temporal pattern of the ECLU reveals that the number of areas with low and medium-low efficiency decreased gradually, while the number of areas with medium-high and high efficiency increased continuously. The magnitude and direction of influencing factors indicates that socioeconomic development level, agricultural science and technology investments, carbon emission reducing, and agricultural pollution control could effectively improve the ECLU. These findings have important implications for promoting high-efficient, low-carbon utilization of cultivated land resources and sustainable regional development in China.
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http://dx.doi.org/10.1016/j.jenvman.2021.112939DOI Listing
June 2021

Iron-Catalyzed, Site-Selective Difluoromethylthiolation (-SCFH) and Difluoromethylselenation (-SeCFH) of Unactivated C(sp)-H Bonds in -Fluoroamides.

Org Lett 2021 Jun 3;23(12):4721-4725. Epub 2021 Jun 3.

College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China.

The iron-catalyzed δ-C(sp)-H bond difluoromethylthiolation and difluoromethylselenation of aliphatic amides with high site selectivity are reported. Essential to the success is the employment of an amide radical formed in situ to activate the inert C(sp)-H bond and the utilization of the easily handled PhSOSCFH and PhSOSeCFH as coupling reagents under mild conditions. This scalable protocol exhibits a broad substrate scope bearing versatile functional groups. Mechanistic studies indicate that the reaction proceeds through -SCFH and -SeCFH radical transfer.
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http://dx.doi.org/10.1021/acs.orglett.1c01443DOI Listing
June 2021

miR-497 targets VEGF signal pathway to regulate proliferation, invasion and migration of hepatocellular carcinoma cells: a primary study using DEC-MRI.

J BUON 2021 Mar-Apr;26(2):418-428

CT Department, The Second Affiliated Hospital of Qiqihar Medical College, Qiqihar 161000, China.

Purpose: To quantify the expression of miR-497 and its target gene VEGF-B in patients with hepatocellular carcinoma (HCC), and microvascular invasion (MVI) to identify their relationship with clinicopathological characteristics and prognosis.

Methods: Imaging data of postoperative cancer and adjacent tissues of HCC patients with MVI diagnosed by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) were retrospectively analyzed. The expression of miR-497 in clinical samples and HepG2 and SMMC-7721 cell lines was quantified by quantitative PCR (Q-PCR). Correlations between miR-497 and patient survival and VEGF-B were explored in TCGA database. The invasion and migration of SMMC-7721 cells were tested by transwell assay. The binding sites between miR-497 and its target gene VEGF-B were verified by dual-luciferase reporter (DLR) assay, and VEGF-B levels were analyzed by western blot (WB).

Results: miR-497 showed a lower expression in HCC patients with MVI than those without MVI. It was also lowly expressed in HCC cell lines compared to normal liver cell lines. The proliferation and migration in HCC cells were inhibited by overexpression of miR-497, which were enhanced after transfection with miR-497 inhibitor. miR-497 had an effect on VEGF-B levels and there was a regulatory relationship between them. miR-497 was able to target VEGF-B and downregulate the receptor of VEGF-B (FLT-1).

Conclusion: miR-497 was lowly expressed in HCC tissues, and its overexpression inhibited invasion and metastasis in HCC cells by suppressing VEGF-B levels. MiR-497 and its target gene VEGF-B are closely associated with the biological function and may serve as prognostic factors of MVI in patients with HCC.
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June 2021

Post-Treatment Sevoflurane Protects Against Hypoxic-Ischemic Brain Injury in Neonatal Rats by Downregulating Histone Methyltransferase G9a and Upregulating Nuclear Factor Erythroid 2-Related Factor 2 (NRF2).

Med Sci Monit 2021 Jun 1;27:e930042. Epub 2021 Jun 1.

Department of Anesthesiology, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China (mainland).

BACKGROUND Perinatal hypoxia and subsequent reduction of cerebral blood flow leads to neonatal hypoxic-ischemic brain injury (HIBI), resulting in severe disability and even death. Preconditioning or post-conditioning with sevoflurane protects against cerebral injury. This study investigated the mechanism of sevoflurane in HIBI. MATERIAL AND METHODS The HIBI model of neonatal rats was established and the model rats were post-treated with sevoflurane. The oxygen-glucose deprivation (OGD) cell model was established, and the OGD cells were transfected with NRF2-siRNA plasmid and post-treated with sevoflurane. The Morris water maze test was used to detect the motor activity, spatial learning, and memory ability of HIBI rats. Histological stainings were performed to observe the area of cerebral infarction, record the number of neurons in the hippocampus, and assess neuron apoptosis. The levels of inflammatory factors were detected by ELISA. The protein levels of histone methyltransferase G9a and histone H3 lysine 9 (H3K9me2) were detected by western blot assay. The apoptosis was detected by flow cytometry. RESULTS Sevoflurane post-treatment significantly shortened the escape latency of HIBI neonatal rats, increased the density of neurons, reduced the area of cerebral infarction, and decreased the levels of inflammatory factors and neuronal apoptosis. Sevoflurane post-treatment decreased G9a and H3K9me2 levels, and G9a level was negatively correlated with NRF2 level. NRF2 silencing reversed the alleviation of sevoflurane post-treatment on OGD-induced cell injury. CONCLUSIONS Sevoflurane post-treatment promotes NRF2 expression by inhibiting G9a and H3K9me2, thus alleviating HIBI in neonatal rats.
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http://dx.doi.org/10.12659/MSM.930042DOI Listing
June 2021

The tyrosine phosphatase SHP2 promotes proliferation and oxaliplatin resistance of colon cancer cells through AKT and ERK.

Biochem Biophys Res Commun 2021 Jul 27;563:1-7. Epub 2021 May 27.

Cancer Institute, The Affiliated Hospital of Qingdao University, Qingdao 266061, Qingdao Cancer Institute, Qingdao University, Qingdao 266061, China. Electronic address:

The SH2 domain-containing phosphatase 2 (SHP2) is a widely expressed protein tyrosine phosphatase, and it is proposed to act as an oncogenic protein. SHP2 is also engaged in drug resistance of a variety of cancers. However, the role of SHP2 in the proliferation and drug resistance of colon cancer cells remains elusive. In this work we determined the effect of SHP2 expression on colon cancer cell proliferation and resistance to oxaliplatin (L-OHP), a commonly used drug in the clinic. Our results show that knockdown of SHP2 decreased and overexpression of SHP2 increased the proliferation of SW480 cells, respectively. Knockdown of SHP2 increased, and overexpression of SHP2 decreased apoptosis of the cells. We selected oxaliplatin-resistant SW480(SW480/L-OHP) and HCT116(HCT116/L-OHP) cells and found that the SHP2 protein level was raised in these drug-resistant cells. The upregulated SHP2 contributed to oxaliplatin resistance of the cells, as knockdown of SHP2 decreased the IC of oxaliplatin and abated proliferation and survival of SW480/L-OHP and HCT116/L-OHP cells in the presence of oxaliplatin. Also, SW480/L-OHP and HCT116/L-OHP cells had increased phosphorylation of AKT and ERK. Inhibition of AKT, ERK, or SHP2 sensitized SW480/L-OHP and HCT116/L-OHP cells to oxaliplatin. Our results indicate that SHP2 contributes oxaliplatin resistance through AKT and ERK. These results also suggest that SHP2-targeting is a potential strategy for overcoming oxaliplatin resistance of colon cancer cells.
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http://dx.doi.org/10.1016/j.bbrc.2021.05.068DOI Listing
July 2021

SEC61G promotes breast cancer development and metastasis via modulating glycolysis and is transcriptionally regulated by E2F1.

Cell Death Dis 2021 May 27;12(6):550. Epub 2021 May 27.

Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Breast cancer is the most common cancer in women and its incidence rates are rapidly increasing in China. Understanding the molecular mechanisms of breast cancer tumorigenesis enables the development of novel therapeutic strategies. SEC61G is a subunit of the endoplasmic reticulum translocon that plays critical roles in various tumors. We aimed to investigate the expression and function of SEC61G in breast cancer. By analyzing The Cancer Genome Atlas breast cancer cohort, we found that SEC61G was highly expressed in breast cancer and predicted poor prognosis of breast cancer patients. Overexpression of SEC61G and its prognostic role was also confirmed in the Nanjing Medical University (NMU) breast cancer cohort. Functionally, we demonstrated that knockdown of SEC61G suppressed breast cancer cell proliferation, migration, invasion, and promoted breast cancer cell apoptosis in vitro. Xenograft breast tumor model revealed that knockdown of SEC61G inhibited breast tumor development in vivo. Furthermore, we demonstrated that SEC61G positively regulated glycolysis in breast cancer cells. Mechanistically, we showed that transcription factor E2F1 directly bound to the promoter of SEC61G and regulated its expression in breast cancer cells. SEC61G overexpression antagonized the effect of E2F1 knockdown in regulating breast cancer cell proliferation, invasion, and apoptosis. Finally, we demonstrated that the E2F1/SEC61G axis regulated glycolysis and chemo-sensitivity of Herceptin in breast cancer cells. Taken together, these results of in vitro and in vivo studies demonstrate that SEC61G promotes breast cancer development and metastasis via modulating glycolysis and is transcriptionally regulated by E2F1, which might be utilized as a promising therapeutic target of breast cancer treatment.
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http://dx.doi.org/10.1038/s41419-021-03797-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155024PMC
May 2021

Association of Team-Based Care and Continuity of Care with Hospitalizations for Veterans with Comorbid Mental and Physical Health Conditions.

J Gen Intern Med 2021 May 23. Epub 2021 May 23.

Department of Acute and Tertiary Care, School of Nursing, University of Pittsburgh, 3500 Victoria Street 336 Victoria Building, Pittsburgh, PA, USA.

Background: Integrating mental health in primary care settings is associated with improved screening and detection of mental illness. In 2010, the Veterans Health Administration launched a patient-centered medical home (PCMH) model nationally across all clinical sites that integrated mental health into primary care-the Patient Aligned Care Team (PACT) initiative. Team-based delivery of continuous primary and mental health care, as found in effective collaborative care models, is thought to be crucial to managing veterans with mental health disorders. The association between clinic implementation of specific aspects of PACT and clinical outcomes of veterans with mental health disorders remains unknown.

Objective: To examine the association between clinic implementation of team-based care and continuity of care and subsequent hospitalizations among veterans with mental health disorders.

Design: Retrospective cohort study.

Patients: A total of 1,444,942 veterans with comorbid mental health disorders and physical health conditions receiving primary care in 831 VA PACT clinics in fiscal year (FY) 2015.

Main Measures: We examined the clinic-level implementation of team-based care and continuity of care in the clinic where veterans received their primary care. Our primary outcome was any hospitalization in the VA or fee-based service in FY2016. We examined the impact of clinic-level implementation of team-based care and continuity of care on having a hospitalization, adjusting for patient demographic, clinical characteristics, and facility characteristics.

Key Results: Veterans receiving care in clinics with the greatest versus lowest quartile of implementation of team-based care had lower rates of hospitalization (8.8% vs. 12.3%; adjusted OR = 0.92, 95% CI 0.85-0.99, p < 0.035). There was not a statistically significant association between clinic-level implementation of continuity of care and hospitalization.

Conclusions: Veterans receiving care in clinics with greater implementation of team-based care had statistically significant lower rates of hospitalization.
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http://dx.doi.org/10.1007/s11606-021-06884-5DOI Listing
May 2021

Surveillance of Antimalarial Drug-Resistance Genes in Imported Isolates From Nigeria in Henan, China, 2012-2019.

Front Cell Infect Microbiol 2021 23;11:644576. Epub 2021 Apr 23.

Department of Parasite Disease Control and Prevention, Henan Provincial Center for Disease Control and Prevention, Henan Key Laboratory of Infectious Disease Microbiology, Zhengzhou, China.

Malaria remains a major public health issue in Nigeria, and Nigeria is one of the main sources of imported malaria in China. Antimalarial drug resistance is a significant obstacle to the control and prevention of malaria globally. The molecular markers associated with antimalarial drug resistance can provide early warnings about the emergence of resistance. The prevalence of antimalarial drug resistant genes and mutants, including , , , , and , was evaluated among the imported isolates from Nigeria in Henan, China, from 2012 to 2019. Among the 167 imported isolates, the wild-type frequency of , , , , and was 98.7, 63.9, 34.8, 3.1, and 3.1%, respectively. The mutation of was rare, with just two nonsynonymous (S693F and Q613H) and two synonymous mutations (C469C and G496G) identified from four isolates. The prevalence of mutation at codon 74-76 decreased year-by-year, while the prevalence of 86Y also decreased significantly with time. The prevalence of and mutants was high. Combined mutations of and had a high prevalence of the quadruple mutant IRN-G (39.0%), followed by the octal mutant IRN-VAGGS (17.0%). These molecular findings update the known data on antimalarial drug-resistance genes and provide supplemental information for Nigeria.
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http://dx.doi.org/10.3389/fcimb.2021.644576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102827PMC
April 2021

Microarray analysis of the time-dependent expression profiles of long non-coding RNAs in the progression of vein graft stenotic disease.

Exp Ther Med 2021 Jun 15;21(6):635. Epub 2021 Apr 15.

Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

Long non-coding RNAs (lncRNAs) have been reported to be involved in various biological processes, including cell proliferation and apoptosis. However, the expression profiles of lncRNAs in patients with vein graft restenosis remain unknown. In the present study, the time-dependent expression profiles of genes in vein bypass grafting models were examined by microarray analysis. A total of 2,572 lncRNAs and 1,652 mRNAs were identified to be persistently significantly differentially expressed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed to investigate the functions of these lncRNAs. A total of 360 lncRNAs and 135 protein-coding genes were predicted to be involved in the vascular remodeling process. Co-expression network analysis revealed the association between 194 lncRNAs and seven associated protein-coding genes, including transforming growth factor-β1, Fes, Yes1 associated transcriptional regulator, sphingosine-1-phosphate receptor 1, Src, insulin receptor and melanoma cell adhesion molecule. Moreover, reverse transcription-quantitative PCR results supported those of the microarray data, and overexpression of AF062402, which regulates the transcription of Src, stimulated the proliferation of primary vascular smooth muscle cells. The findings of the present study may facilitate the development of novel therapeutic targets for vein graft restenosis and may help to improve the prognosis of patients following coronary artery bypass grafting.
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http://dx.doi.org/10.3892/etm.2021.10067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097238PMC
June 2021

Dasatinib inhibits proliferation of liver cancer cells, but activation of Akt/mTOR compromises dasatinib as a cancer drug.

Acta Biochim Biophys Sin (Shanghai) 2021 May 7. Epub 2021 May 7.

Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China.

Dasatinib is a multi-target protein tyrosine kinase inhibitor. Due to its potent inhibition of Src, Abl, the platelet-derived growth factor receptor (PDGFR) family kinases, and other oncogenic kinases, it has been investigated as a targeted therapy for a broad spectrum of cancer types. However, its efficacy has not been significantly extended beyond leukemia. The mechanism of resistance to dasatinib in a wide array of cancers is not clear. In the present study, we investigated the effect of dasatinib on hepatocellular carcinoma cell growth and explored the underlying mechanisms. Our results showed that dasatinib potently inhibited the proliferation of SNU-449 cells, but not that of other cell lines, such as SK-Hep-1, even though it inhibited the phosphorylation of Src on both negative and positive regulation sites in all these cells. Dasatinib activated the phosphoinositide-dependent protein kinase1 (PDK1)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway in SK-Hep-1 cells, but not in SNU-449 cells. Blocking the Akt/mTOR signaling pathway strongly promoted the efficacy of dasatinib in SK-Hep-1 cells. In SNU-449 cells, dasatinib promoted apoptosis and the cleavage of caspase-3 and caspase-7, induced cell cycle arrest in the G1 phase, and inhibited the expression of Cyclin-dependent kinase (CDK4)/6/CyclinD1 complex. These findings demonstrate that dasatinib exerts its anti-proliferative effect on hepatocellular cell proliferation by blocking the Src family kinases; however, it causes Akt activation, which compromises dasatinib as an anti-cancer drug.
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http://dx.doi.org/10.1093/abbs/gmab061DOI Listing
May 2021

[Corrigendum] High PLK4 expression promotes tumor progression and induces epithelial‑mesenchymal transition by regulating the Wnt/β‑catenin signaling pathway in colorectal cancer.

Int J Oncol 2021 Jun 6;58(6). Epub 2021 May 6.

Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.

Subsequently to the publication of the above article, the author realized that Fig. 5 on p. 486 contained some errors on account of the figure having been compiled incorrectly; essentially, the published version of the figure contained incorrect images for the panels presented in Fig. 5C and E. The authors were able to re‑examine their original data, and identify the data that was intended to have been shown for these figure parts. The corrected version of Fig. 5 is shown on the next page, featuring the correct data for Fig. 5C and E, including new bar charts showing the quantification of these data. The authors confirm that these data continue to support the main conclusions presented in their paper, and are grateful to the Editor of for allowing them this opportunity to publish a Corrigendum. They also apologize to the readership for any inconvenience caused. [the original article was published in 54: 479‑490, 2019; DOI: 10.3892/ijo.2018.4659].
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http://dx.doi.org/10.3892/ijo.2021.5213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104922PMC
June 2021

Comparison of GFAP and UCH-L1 Measurements from Two Prototype Assays: The Abbott i-STAT and ARCHITECT Assays.

Neurotrauma Rep 2021 7;2(1):193-199. Epub 2021 Apr 7.

Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Glial fibrillary acidic protein (GFAP) and ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) may aid in the evaluation of traumatic brain injury (TBI). The objective of this analysis was to compare GFAP and UCH-L1 values measured using a handheld device compared with a core laboratory platform. We analyzed plasma samples from patients with TBI and healthy controls enrolled in the Transforming Research and Clinical Knowledge in TBI (TRACK-TBI) cohort study. GFAP and UCH-L1 were measured twice in each subject using prototype assays, first with the Abbott i-STAT™ handheld device, and second with the Abbott ARCHITECT platform. We then quantified the agreement in biomarker values obtained using these two methods. GFAP and UCH-L1 were measured twice in 570 and 572 samples, respectively. GFAP values measured by the ARCHITECT platform (median 143.3 [interquartile range (IQR): 19.8-925.8] pg/mL) were higher than values measured by the i-STAT (median 116.0 [IQR: 9.2-856.5] pg/mL). GFAP values from the two platforms were strongly correlated ( = 0.985). Similarly, UCH-L1 values measured by the ARCHITECT platform (median 163.9 [IQR: 82.5-412.4] pg/mL) were higher than values measured by the i-STAT (median 122.5 [IQR: 63.0-297.3] pg/mL). UCH-L1 values from the two platforms were strongly correlated ( = 0.933). Passing-Bablok regression equations were developed to estimate the relationship between the two platforms, specifically to predict i-STAT values from the ARCHITECT platform. GFAP and UCH-L1 values measured using the prototype assays on the Abbott i-STAT and ARCHITECT platforms are strongly correlated and values from either platform may be converted to the other.
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http://dx.doi.org/10.1089/neur.2020.0037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086519PMC
April 2021

Surgical treatment of children with drug-resistant epilepsy involving the Rolandic area.

Epileptic Disord 2021 Apr;23(2):376-384

Pediatric Epilepsy Center, Peking University First Hospital, No. 1 Xi'an Men Street, Xicheng District, Beijing 100034, China, Department of Pediatrics, Peking University First Hospital, No. 1 Xi'an Men Street, Xicheng District, Beijing 100034, China.

We retrospectively analysed the clinical features and prognostic factors of surgery in children with drug-resistant epilepsy involving the Rolandic area, and the relationship between the stable compound muscle action potentials (CMAPs) of intraoperative neurophysiological monitoring (IONM) and good motor function outcomes postoperatively. A study was conducted on the clinical data of 91 patients with epilepsy who underwent epilepsy surgery involving the Rolandic area and IONM from November 2015 to February 2019. In total, 91 patients were included in this study. The median age at seizure onset was 1.3 years old. The median age at surgery was 4.4 years old. Twenty-seven patients (29.7%), with age at onset below three years old, had epileptic spasms. The central operculum was the most common surgical region in 52 patients (57.1%). The most common pathology was focal cortical dysplasia (FCD) in 67 patients. At the last follow-up visit, 69 patients (75.8%) were seizure-free. Interictal epileptiform discharges in the Rolandic area were associated with good seizure outcome (p=0.016). Out of 91 patients, successful IONM was performed in 88 patients (96.7%). Stable CMAP was seen in 79 of 88 patients (89.8%), and irreversible disappearance of CMAP was seen in nine patients (10.2%). New permanent motor deficit was observed in 13 of 88 patients (14.8%). There was a significant correlation between stable CMAP and good motor function outcome (p<0.001). This is the largest reported cohort of children with drug-resistant epilepsy involving the Rolandic area who received surgery from a single centre. Epileptic spasms were only observed in young children with age at onset below three years old. The major aetiology was FCD. The rate of seizure freedom was 75.8%. Epileptiform discharges in the Rolandic area were the main prognostic factor affecting surgical outcome. Stable CMAP can predict good motor function outcome postoperatively.
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http://dx.doi.org/10.1684/epd.2021.1279DOI Listing
April 2021

Perceiving Social-Emotional Volatility and Triggered Causes of COVID-19.

Int J Environ Res Public Health 2021 04 26;18(9). Epub 2021 Apr 26.

College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China.

Health support has been sought by the public from online social media after the outbreak of novel coronavirus disease 2019 (COVID-19). In addition to the physical symptoms caused by the virus, there are adverse impacts on psychological responses. Therefore, precisely capturing the public emotions becomes crucial to providing adequate support. By constructing a domain-specific COVID-19 public health emergency discrete emotion lexicon, we utilized one million COVID-19 theme texts from the Chinese online social platform Weibo to analyze social-emotional volatility. Based on computed emotional valence, we proposed a public emotional perception model that achieves: (1) targeting of public emotion abrupt time points using an LSTM-based attention encoder-decoder (LAED) mechanism for emotional time-series, and (2) backtracking of specific triggered causes of abnormal volatility in a cognitive emotional arousal path. Experimental results prove that our model provides a solid research basis for enhancing social-emotional security outcomes.
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http://dx.doi.org/10.3390/ijerph18094591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123597PMC
April 2021

The non-canonical functions of HIF prolyl hydroxylases and their dual roles in cancer.

Int J Biochem Cell Biol 2021 Jun 21;135:105982. Epub 2021 Apr 21.

Cancer Institute, The Affiliated Hospital of Qingdao University, Cancer Institute, Qingdao University, Qingdao, 266061, China. Electronic address:

The hypoxia-inducible factor (HIF) prolyl hydroxylases (PHDs) are dioxygenases using oxygen and 2-oxoglutarate as co-substrates. Under normoxia, PHDs hydroxylate the conserved prolyl residues of HIFα, leading to HIFα degradation. In hypoxia PHDs are inactivated, which results in HIFα accumulation. The accumulated HIFα enters nucleus and initiates gene transcription. Many studies have shown that PHDs have substrates other than HIFα, implying that they have HIF-independent non-canonical functions. Besides modulating protein stability, the PHDs-mediated prolyl hydroxylation affects protein-protein interaction and protein activity for alternative substrates. Increasing evidence indicates that PHDs also have hydroxylase-independent functions. They influence protein stability, enzyme activity, and protein-protein interaction in a hydroxylase-independent manner. These findings highlight the functional diversity and complexity of PHDs. Due to having inhibitory activity on HIFα, PHDs are proposed to act as tumor suppressors. However, research shows that PHDs exert either tumor-promoting or tumor-suppressing features. Here, we try to summarize the current understanding of PHDs hydroxylase-dependent and -independent functions and their roles in cancer.
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http://dx.doi.org/10.1016/j.biocel.2021.105982DOI Listing
June 2021

Manganese-deposited iron oxide promotes tumor-responsive ferroptosis that synergizes the apoptosis of cisplatin.

Theranostics 2021 13;11(11):5418-5429. Epub 2021 Mar 13.

Department of Pharmacy, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine; Department of Chemistry, University of Science and Technology of China, Hefei 230001, China.

Ferroptosis is a form of iron-dependent programmed cell death that differs from apoptosis with regards to both mechanism and cell morphology. Therefore, ferroptotic-based cancer therapy has shown significant potential to overcome the weaknesses of conventional therapeutics mediated by apoptosis pathways. Effective ferroptosis can be induced by the intracellular Fenton reaction that is dependent on the adequate supply of iron ions and HO in cells. However, these are often insufficient due to intrinsic cellular regulation. In this study, we designed a cisplatin prodrug-loaded manganese-deposited iron oxide nanoplatform (Pt-FMO) to trigger intracellular cascade reactions that lead to generation of reactive oxygen species (ROS) to enhance ferroptotic effect. The Pt-FMO causes the tumor microenvironment responsive to release manganese, iron ions and Pt-drugs. As manganese is an element that is able to catalyze the Fenton reaction more effectively than iron, coupled with the Pt-drugs that can promote generation of HO in cells, the Pt-FMO is expected to significantly strengthen catalysis of the Fenton reaction, which favors the ferroptotic effect. Moreover, the Pt-drugs will eventually function as cisplatin. Thus, Pt-FMO is an ideal candidate for tumor ferroptotic combined with apoptotic treatment. results demonstrated that, at a dosage of only 8.89% Pt content, Pt-FMO is able to achieve a similar treatment effect as cisplatin. Hence, Pt-FMO exhibited significantly lower systemic toxicity compared to cisplatin. Additionally, Pt-FMO exhibits effective -weighted MRI enhancement for tumor imaging. The Pt-FMO nanoplatform is designed to introduce mutual beneficial cascade reactions for promoting ferroptosis and apoptosis in combination with tumor MRI. The Pt-FMO system, which causes ferroptosis combined with apoptosis, can efficiently induce tumor cell death.
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http://dx.doi.org/10.7150/thno.53346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039957PMC
March 2021

Rapid responses of adipocytes to iron overload increase serum TG level by decreasing adiponectin.

J Cell Physiol 2021 Apr 14. Epub 2021 Apr 14.

Department of Nutrition, Second Military Medical University, Shanghai, China.

Iron overload is tightly connected with metabolic disorders. Excess iron in the adipose and its roles in dyslipidemia are of interest to be identified. In acute iron overload mice receiving intraperitoneal injection of 100 mg/kg/day dextran-iron for 5 days, the epididymis adipose showed a remarkable increase in iron. Serum triglyceride and low-density lipoprotein cholesterol (LDL-C) levels were increased and high-density lipoprotein cholesterol (HDL-C) level was decreased, while serum alkaline phosphatase, aspartate aminotransferase, glucose, and insulin were not affected. The serum-cytokine-microarray showed that adipocytokines, including adiponectin, leptin, and resistin were significantly decreased. Other serum cytokines, including pro-insulin cytokines, inflammatory cytokines, chemokines, and growth factors were not changed, except that ghrelin and chemokine RANTES were increased. Iron overload decreased expressions of adiponectin and leptin both in vivo and in vitro. Intraperitoneal injection of recombinant leptin at 1 μg/g in acute iron overload mice had no significant effects on serum levels of TC, TG, HDL-C, and LDL-C, while intraperitoneal injection of recombinant adiponectin at 3 μg/g partially restored serum TG level through improving activities of lipoprotein lipase and hepatic lipase, but abnormal serum LDL-C and HDL-C were not redressed, suggesting other mechanisms also existed. In conclusion, the adipose responds to iron overload at an early stage to interfere with lipid metabolism by secreting adipocytokines, which may further affect glucose metabolism, inflammation, and other iron overload-induced effects on the body.
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http://dx.doi.org/10.1002/jcp.30391DOI Listing
April 2021

Propofol modulates the proliferation, invasion and migration of bladder cancer cells through the miR‑145‑5p/TOP2A axis.

Mol Med Rep 2021 Jun 13;23(6). Epub 2021 Apr 13.

Department of Anesthesiology, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041, P.R. China.

Propofol‑based anesthesia has been reported to reduce the recurrence and metastasis of a number of cancer types following surgical resection. However, the effects of propofol in bladder cancer (BC) are yet to be fully elucidated. The aim of the present study was to investigate the functions of propofol in BC and their underlying mechanisms. In the study, the expression of microRNA (miR)‑145‑5p in BC tissues and cell lines was evaluated using reverse transcription‑quantitative PCR, and the effects of propofol on BC cells were determined using cell viability, wound healing and Transwell cell invasion assays, bioinformatics analysis, western blotting, immunohistochemistry and tumor xenograft models. It was found that propofol significantly suppressed the proliferation, migration and invasion of BC cells . In addition, propofol induced miR‑145‑5p expression in a time‑dependent manner, and miR‑145‑5p knockdown attenuated the inhibitory effects of propofol on the proliferation, migration and invasion of BC cells. Topoisomerase II α (TOP2A) was a direct target of miR‑145‑5p, and silencing TOP2A reversed the effects of miR‑145‑5p knockdown in propofol‑treated cells. Furthermore, propofol suppressed tumor xenograft growth, which was partially attenuated by miR‑145‑5p knockdown. The present study provided novel insight into the advantages of surgical intervention with propofol anesthesia in patients with BC.
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http://dx.doi.org/10.3892/mmr.2021.12078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060790PMC
June 2021

Rapid Detection of SARS-CoV-2 Virus Using Dual Reverse Transcriptional Colorimetric Loop-Mediated Isothermal Amplification.

ACS Omega 2021 Apr 22;6(13):8837-8849. Epub 2021 Mar 22.

Laboratory for Quality Control and Traceability of Food, Tianjin Normal University, Tianjin 300387, P. R. China.

The outbreak and pandemic of COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has developed into a public health emergency of international concern. The rapid and accurate detection of the virus is a critical means to prevent and control the disease. Herein, we provide a novel, rapid, and simple approach, named dual reverse transcriptional colorimetric loop-mediated isothermal amplification (dRT-cLAMP) assay, to accelerate the detection of the SARS-CoV-2 virus without using expensive equipment. The result of this assay is shown by color change and is easily detected by the naked eye. To improve the detection accuracy, we included two primer sets that specifically target the viral and genes in the same reaction mixture. Our assay can detect the synthesized SARS-CoV-2 and genes at a low level of 100 copies/μL. Sequence alignment analysis of the two synthesized genes and those of 9968 published SARS-CoV-2 genomes and 17 genomes of other pathogens from the same infection site or similar symptoms as COVID-19 revealed that the primers for the dRT-cLAMP assay are highly specific. Our assay of 27 clinical samples of SARS-CoV-2 virus and 27 standard-added environmental simulation samples demonstrated that compared to the commercial kits, the consistency of the positive, negative, and probable clinical samples was 100, 92.31, and 44.44%, respectively. Moreover, our results showed that the positive, but not negative, standard-added samples displayed a naked-eye-detectable color change. Together, our results demonstrate that the dRT-cLAMP assay is a feasible detection assay for SARS-CoV-2 virus and is of great significance since rapid onsite detection of the virus is urgently needed at the ports of entry, health care centers, and for internationally traded goods.
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http://dx.doi.org/10.1021/acsomega.0c05781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008787PMC
April 2021

Exosomal analysis: Advances in biosensor technology.

Clin Chim Acta 2021 Jul 31;518:142-150. Epub 2021 Mar 31.

Department of Blood Transfusion, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China. Electronic address:

Exosomes, a subtype of extracellular vesicle secreted by cells, have been a subject of intense research interest. Unfortunately, a simple and reliable method to separate exosomes has yet to be developed. As can be expected, the lack of a standardized method for extraction and purification has contributed to suboptimal inter-laboratory correlation and difficulty in comparison studies. Traditional techniques such as centrifugation, immunoaffinity and size exclusion chromatography, suffer from low purity and tend to be labor intensive thus making their use limited. To mitigate these drawbacks, an integrated biosensor-based exosome separation and detection has recently been developed. In this review, we examine five biosensors that use a variety of detection technology (colorimetric, fluorescent, surface plasmon resonance, surface-enhanced Raman scattering and electrochemical) and propose thoughts on standardization of exosomal analysis.
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http://dx.doi.org/10.1016/j.cca.2021.03.026DOI Listing
July 2021

Medication Treatment of Active Opioid Use Disorder in Veterans With Cirrhosis.

Am J Gastroenterol 2021 Mar 30. Epub 2021 Mar 30.

Center for Health Equity Research and Promotion, VA Pittsburgh Healthcare System, Pittsburgh, Pennsylvania, USA; Department of Medicine, Division of Gastroenterology, Hepatology, and Nutrition, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA; Department of Surgery, Division of Transplant Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA; Department of Biostatistics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA; Division of General Internal Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA; Center for Pharmaceutical Policy and Prescribing, Health Policy Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Department of Veterans Affairs, Palo Alto Health Care System, Palo Alto, California, USA; Gastroenterology Section, VA Long Beach Healthcare System, Long Beach, California, USA; Division of Gastroenterology, Department of Medicine, University of California, Irvine, California, USA; Centers for Value Based Pharmacy Initiatives and High Value Health Care, UPMC Health Plan Insurance Division, Pittsburgh, Pennsylvania.

Introduction: Although opioid use disorder (OUD) is common in patients with cirrhosis, it is unclear how medication treatment for OUD (MOUD) is used in this population. We aimed to assess the factors associated with MOUD and mortality in a cohort of Veterans with cirrhosis and OUD.

Methods: Within the Veterans Health Administration Corporate Data Warehouse, we developed a cohort of Veterans with cirrhosis and active OUD, using 2 outpatient or 1 inpatient International Classification of Diseases, ninth revision codes from 2011 to 2015 to define each condition. We assessed MOUD initiation with methadone or buprenorphine over the 180 days following the first OUD International Classification of Diseases, ninth revision code in the study period. We fit multivariable regression models to assess the association of sociodemographic and clinical factors with receiving MOUD and the associations between MOUD and subsequent clinical outcomes, including new hepatic decompensation and mortality.

Results: Among 5,600 Veterans meeting criteria for active OUD and cirrhosis, 722 (13%) were prescribed MOUD over 180 days of follow-up. In multivariable modeling, MOUD was significantly, positively associated with age (adjusted odds ratio [AOR] per year: 1.04, 95% confidence interval (CI): 1.01-1.07), hepatitis C virus (AOR = 2.15, 95% CI = 1.37-3.35), and other substance use disorders (AOR = 1.47, 95% CI = 1.05-2.04) negatively associated with alcohol use disorder (AOR = 0.70, 95% CI = 0.52-0.95), opioid prescription (AOR = 0.51, 95% CI = 0.38-0.70), and schizophrenia (AOR = 0.59, 95% CI = 0.37-0.95). MOUD was not significantly associated with mortality (adjusted hazards ratio = 1.20, 95% CI = 0.95-1.52) or new hepatic decompensation (OR = 0.57, CI = 0.30-1.09).

Discussion: Few Veterans with active OUD and cirrhosis received MOUD, and those with alcohol use disorder, schizophrenia, and previous prescriptions for opioids were least likely to receive these effective therapies.
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http://dx.doi.org/10.14309/ajg.0000000000001228DOI Listing
March 2021

Pyruvate accumulation may contribute to acceleration-induced impairment of physical and cognitive abilities: an experimental study.

Biosci Rep 2021 Apr;41(4)

Department of Nutrition and Food Hygiene, Faculty of Navy Medicine, Naval Medical University, 800 Xiangyin Road, Shanghai 200433, China.

Background: Fatigue can be induced after acceleration exposure, however its mechanism is still unclear. The aim of the present study was to examine whether metabolites' changes can decrease cognitive and physical function after acceleration.

Methods: Graybiel scale and Fatigue Self-rating scale were used to assess the seasickness and fatigue degrees of 87 male seafarers respectively after sailing. To test the effect of pyruvate on cognitive and physical functions, five different doses of pyruvate were administrated into rats. Insulin can reduce the accumulation of pyruvate. To observe the insulin effect on pyruvate, cognitive and physical functions after acceleration, insulin administration or treatment of promoting insulin secretion was used. Physical and cognitive functions were assessed using open field test (OFT), morris water maze (MWM) and loaded swimming test (LST) in animals.

Results: Physical and cognitive abilities were decreased obviously, and serum pyruvate increased mostly in human and rats after acceleration. Compared with vehicle group, physical and cognitive abilities were significantly decreased after pyruvate administration. Besides, we found a significant decline in adenosine triphosphate (ATP) concentration and pyruvate dehydrogenase (PDH) activity in the hippocampus, prefrontal cortex, liver, and muscle of rats treated with acceleration or pyruvate injection, while insulin administration or treatment of promoting insulin secretion markedly alleviated this decline and the impairment of physical and cognitive abilities, compared with the control group.

Conclusion: Our results indicate that pyruvate has a negative effect on physical and cognitive abilities after acceleration. Insulin can inhibit pyruvate accumulation and cognitive and physical function after acceleration exposure.
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http://dx.doi.org/10.1042/BSR20204284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047541PMC
April 2021

Lyophilized powder of mesenchymal stem cell supernatant attenuates acute lung injury through the IL-6-p-STAT3-p63-JAG2 pathway.

Stem Cell Res Ther 2021 Mar 29;12(1):216. Epub 2021 Mar 29.

Department of Pulmonary and Critical Care Medicine, Shanghai Respiratory Research Institute, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Background: Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are syndromes of acute respiratory failure with extremely high mortality and few effective treatments. Mesenchymal stem cells (MSCs) may reportedly contribute to tissue repair in ALI and ARDS. However, applications of MSCs have been restricted due to safety considerations and limitations in terms of large-scale production and industrial delivery. Alternatively, the MSC secretome has been considered promising for use in therapeutic approaches and has been advanced in pre-clinical and clinical trials. Furthermore, the MSC secretome can be freeze-dried into a stable and ready-to-use supernatant lyophilized powder (SLP) form. Currently, there are no studies on the role of MSC SLP in ALI.

Methods: Intratracheal bleomycin was used to induce ALI in mice, and intratracheal MSC SLP was administered as a treatment. Histopathological assessment was performed by hematoxylin and eosin, immunohistochemistry, and immunofluorescence staining. Apoptosis, inflammatory infiltration, immunological cell counts, cytokine levels, and mRNA- and protein-expression levels of relevant targets were measured by performing terminal deoxynucleotidyl transferase dUTP nick-end labeling assays, determining total cell and protein levels in bronchoalveolar lavage fluids, flow cytometry, multiple cytokine-detection techniques, and reverse transcriptase-quantitative polymerase chain reaction and western blot analysis, respectively.

Results: We found that intratracheal MSC SLP considerably promoted cell survival, inhibited epithelial cell apoptosis, attenuated inflammatory cell recruitment, and reversed immunological imbalances induced by bleomycin. MSC SLP inhibited the interleukin 6-phosphorylated signal transducer and activator of transcription signaling pathway to activate tumor protein 63-jagged 2 signaling in basal cells, suppress T helper 17 cell differentiation, promote p63 cell proliferation and lung damage repair, and attenuate inflammatory responses.

Conclusions: MSC SLP ameliorated ALI by activating p63 and promoting p63 cell proliferation and the repair of damaged epithelial cells. The findings of this study also shed insight into ALI pathogenesis and imply that MSC SLP shows considerable therapeutic promise for treating ALI and ARDS.
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http://dx.doi.org/10.1186/s13287-021-02276-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008635PMC
March 2021

Higher postoperative plasma EV PD-L1 predicts poor survival in patients with gastric cancer.

J Immunother Cancer 2021 Mar;9(3)

Department of Biochemistry and Molecular Biology, School of Basic Medicine, Shanxi Key Laboratory of Birth Defects and Cell Regeneration, Key Laboratory of Cellular Physiology (Shanxi Medical University) of Ministry of Education, Shanxi Medical University, Taiyuan, China

Background: The satisfactory prognostic indicator of gastric cancer (GC) patients after surgery is still lacking. Perioperative plasma extracellular vesicular programmed cell death ligand-1 (ePD-L1) has been demonstrated as a potential prognosis biomarker in many types of cancers. The prognostic value of postoperative plasma ePD-L1 has not been characterized.

Methods: We evaluated the prognostic value of preoperative, postoperative and change in plasma ePD-L1, as well as plasma soluble PD-L1, in short-term survival of GC patients after surgery. The Kaplan-Meier survival model and Cox proportional hazards models for both univariate and multivariate analyzes were used. And the comparison between postoperative ePD-L1 and conventional serum biomarkers (carcinoembryonic antigen (CEA), cancer antigen 19-9 (CA19-9) and CA72-4) in prognostic of GC patients was made.

Results: The prognostic value of postoperative ePD-L1 is superior to that of preoperative ePD-L1 on GC patients after resection, and also superior to that of conventional serum biomarkers (CEA, CA19-9 and CA72-4). The levels of postoperative ePD-L1 and ePD-L1 change are independent prognostic factors for overall survival and recurrence free survival of GC patients. High plasma level of postoperative ePD-L1 correlates significantly with poor survival, while high change in ePD-L1 level brings the significant survival benefit.

Conclusions: The level of plasma postoperative ePD-L1 could be considered as a candidate prognostic biomarker of GC patients after resection.
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http://dx.doi.org/10.1136/jitc-2020-002218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986771PMC
March 2021

New insight into quinones triggered ferrate in-situ synthesized polynuclear Fe-hydroxyl complex for enhancing interfacial adsorption in highly efficient removal of natural organic matter.

Sci Total Environ 2021 May 23;770:144844. Epub 2021 Jan 23.

School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; State Key Laboratory of Separation Membranes and Membrane Processes, Tiangong University, Tianjin 300387, China.

In this study, the effects of quinone on the formation of in-situ synthesized polynuclear Fe-hydroxide (PnFe-H) from ferrate activation and enhanced degradation of organics were investigated by in-situ UV linear differential absorbance spectra for the first time. Results indicated benzoquinone (BQ) efficiently activated ferrate for the flocculation of humic acid (HA) that the flocculation reactions rate constants in Fe(VI)-0.1 mM BQ was 3.3 times as much as the blank. Interestingly, quenching studies suggested PnFe-H derived from the high-valence iron species which were the active components by BQ activation, was proved the vital factor for removing of HA. According to the analysis of interaction energy, BQ promoted FeOH converted to Fe(OH) and Fe(OH) which weakened the polar property and increased hydrophobicity of compounds, further benefited for adsorption with lower Lifshitz-van del Waals (LW) and Lewis acid-base (AB) interfacial energy between PnFe-H-contaminant compounds. However, excessive BQ reduced freshly particulate Fe(III) to Fe(II), weakened the PnFe-H flocculation performance which retarded the transformation of iron species. In addition, the effects of HA concentration were also studied due to the existent of functional quinone-like moieties. The contribution of PnFe-H flocculation removal on the total removal (Re/Re) improved from 2.6% to 17.09% with Fe(VI)/HA from 0.1 to 1.12. Fe(VI) sufficient oxidized electron-rich moieties and decreased the aromaticity due to π bond was broken, further cooperated with PnFe-H captured small fragment particles by sweep flocculation that Fe(VI) self-accelerating decay produced more Fe(III). The research elucidated a new insight into of ferrate activation by quinone which could expand our knowledge of activation pathway, further regulate the relationship between oxidation and flocculation for enhancing organic and colloidal particle removal in practical application.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144844DOI Listing
May 2021

Adsorption of condensable particulate matter from coal-fired flue gas by activated carbon.

Sci Total Environ 2021 Jul 5;778:146245. Epub 2021 Mar 5.

National Engineering Laboratory for Reducing Emissions from Coal Combustion, Engineering Research Center of Environmental Thermal Technology of Ministry of Education, Shandong Key Laboratory of Energy Carbon Reduction and Resource Utilization, School of Energy and Power Engineering, Shandong University, Jinan, Shandong 250061, China.

Condensable particulate matter (CPM) is a special kind of primary particulate matter and is in a gaseous state before discharge. After discharge, it rapidly forms liquid or solid particles through atmospheric dilution and cooling, which are harmful to the environment and human health. However, current research on controlling CPM is lacking. Therefore, the adsorption effects of activated carbons (ACs) on CPM at different temperatures were studied using EPA Method 202. Results showed that the removal efficiency range of CPM at 90 °C by ACs could reach 19%-22%. The removal efficiency of the inorganic fraction was higher than that of the organic fraction. ACs had obvious adsorption effects on Cl, NH, and Hg in CPM but had marginal adsorption effects on SO, NO, and other metal elements in CPM. ACs had prominent adsorption effects on extremely toxic aromatic compounds in CPM. At a flue gas temperature of 35-170 °C, the efficiency of CPM removal through AC adsorption could increase with decreasing flue gas temperature, and this effect was more obvious during the adsorption of inorganic fractions. In addition, the efficiency of CPM removal through condensation and adsorption could reach up to 51% at 35 °C when flue gas at 130 °C was used as the initial flue gas.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146245DOI Listing
July 2021

The immune landscape during the tumorigenesis of cervical cancer.

Cancer Med 2021 04 10;10(7):2380-2395. Epub 2021 Mar 10.

Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.

Objective: Deciphering the determinants of the intralesional immune reaction in cervical carcinogenesis may be conducive to improving the understanding of the disease and then improve outcomes.

Methods: Public gene-expression data and full clinical annotation were searched in Gene Expression Omnibus in the joint analysis of the array-based four eligible cohorts. The infiltrating estimation was quantified using microenvironment cell populations-counter algorithm and absolute-mode CIBERSORT and verified by flow cytometry analysis. An unsupervised classification on immune genes strongly associated with progression, designated by linear mixed-effects regression. We determined immune response and signaling features of the different developmental stages and immune phenotypes by functional annotation and systematically correlated the expression of immune checkpoints with cell-infiltrating characteristics.

Results: We identified the lesion-intrinsic immunosuppression mechanism was triggered at precancerous stages, such as genome instability and mutation, aerobic glycolysis, activation of proto-oncogene pathways and so forth. Predominant innate and adoptive cells were increasing from normalcy to cancer (B cell, total T cell, regulatory T cells [Tregs], monocytes, neutrophils, and M2-like macrophages) together with the decrease of CD4 T cell and CD8 T cell through the development of cervical cancer. Immune escape initiated on the expression of immunosuppressive molecules from high-grade squamous intraepithelial lesions (HSIL) and culminated in squamous cell carcinoma (SCC). Of note, the expression of immune checkpoints was escalated in the immune-hot and immune-warm phenotype largely encompassed by HSIL and SCC under the stress of both activated and suppressive immune responses.

Conclusions: Immune surveillance is unleashing from low-grade squamous intraepithelial lesions onwards and immune-suppression mechanisms are triggered in HSIL. Thorough knowledge of the immune changing pattern during cervical tumorigenesis contributes to finding the potential therapeutic targets to susceptive patients towards immune checkpoints inhibitors.
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http://dx.doi.org/10.1002/cam4.3833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982625PMC
April 2021

Characterizing gangliosides in six sea cucumber species by HILIC-ESI-MS/MS.

Food Chem 2021 Aug 23;352:129379. Epub 2021 Feb 23.

College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao 266003, Shandong Province, China. Electronic address:

An HILIC-ESI-MS/MS method was established to analyze ganglioside (GLS) in sea cucumbers. In total, 17 GLS subclasses were detected in six sea cucumber species. The basic sea cucumber GLSs (SC-GLSs) were elucidated as NeuGc2-6Glc1-1Cer (SC-GM). The polymerization degree of the sialic acid (Sia) of SC-GLSs can be up to 4, and the linkage among Sias was mostly determined to be 2-8 or 2-11. Neu5Gc, sulfated and fucosylated NeuGc prevalently existed in SC-GLSs. Moreover, a new SC-GLSs structure with phosphoinositidyled Sia was first observed in Bohadschia marmorata. For the first time, we demonstrated that the content of SC-GD, which is the dominant GLS in sea cucumbers, was 27-67%. Minor GLSs characterized as SC-GT(Neu5GcMe) and SC-GQ(Neu5GcMe) were also discovered. Additionally, SC-GD and SC-GD(1S) could significantly promote the differentiation of PC12 cells with structure-selectivity (p < 0.05). Our results provide insights into SC-GLSs to elucidate their Sia substituent and core saccharide chain linkage.
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http://dx.doi.org/10.1016/j.foodchem.2021.129379DOI Listing
August 2021

Chidamide induces apoptosis in DLBCL cells by suppressing the HDACs/STAT3/Bcl‑2 pathway.

Mol Med Rep 2021 May 2;23(5). Epub 2021 Mar 2.

Department of Biochemistry and Molecular Biology, Key Laboratory of Cellular Physiology of Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.

Diffuse large B‑cell lymphoma (DLBCL) is a highly heterogeneous malignant tumor type, and epigenetic modifications such as acetylation or deacetylation serve vital roles in its development. Chidamide, a novel histone deacetylase inhibitor, exerts an anticancer effect against various types of cancer. The present study aimed to evaluate the cellular effect of chidamide on a number of DLBCL cell lines and to investigate its underlying mechanism. The results demonstrated that chidamide induced the death of these cells in a concentration‑(0‑30 µmol/l) and time‑dependent (24‑72 h) manner, as determined using the Cell Counting Kit‑8 cell viability assay. Moreover, chidamide promoted cellular apoptosis, which was identified via flow cytometry and western blot analysis, with an increase in cleaved caspase‑3 expression and a decrease in Bcl‑2 expression. Chidamide treatment also decreased the expression level of STAT3 and its phosphorylation, which was accompanied by the downregulation of a class‑I histone deacetylase (HDAC) inhibitor, chidamide. Collectively, these data suggested that chidamide can be a potent therapeutic agent to treat DLBCL by inducing the apoptotic death of DLBCL cells by inhibiting the HDACs/STAT3/Bcl‑2 pathway.
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http://dx.doi.org/10.3892/mmr.2021.11947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974270PMC
May 2021