Publications by authors named "Hongwei Xu"

219 Publications

Effect of Interventional Therapy on Iliac Venous Compression Syndrome Evaluated and Diagnosed by Artificial Intelligence Algorithm-Based Ultrasound Images.

J Healthc Eng 2021 22;2021:5755671. Epub 2021 Jul 22.

Department of Interventional Vascular Surgery, Chengde City Central Hospital, Chengde 067000, Hebei, China.

In order to explore the efficacy of using artificial intelligence (AI) algorithm-based ultrasound images to diagnose iliac vein compression syndrome (IVCS) and assist clinicians in the diagnosis of diseases, the characteristics of vein imaging in patients with IVCS were summarized. After ultrasound image acquisition, the image data were preprocessed to construct a deep learning model to realize the position detection of venous compression and the recognition of benign and malignant lesions. In addition, a dataset was built for model evaluation. The data came from patients with thrombotic chronic venous disease (CVD) and deep vein thrombosis (DVT) in hospital. The image feature group of IVCS extracted by cavity convolution was the artificial intelligence algorithm imaging group, and the ultrasound images were directly taken as the control group without processing. Digital subtraction angiography (DSA) was performed to check the patient's veins one week in advance. Then, the patients were rolled into the AI algorithm imaging group and control group, and the correlation between May-Thurner syndrome (MTS) and AI algorithm imaging was analyzed based on DSA and ultrasound results. Satisfaction of intestinal venous stenosis (or occlusion) or formation of collateral circulation was used as a diagnostic index for MTS. Ultrasound showed that the AI algorithm imaging group had a higher percentage of good treatment effects than that of the control group. The call-up rate of the DMRF-convolutional neural network (CNN), precision, and accuracy were all superior to those of the control group. In addition, the degree of venous swelling of patients in the artificial intelligence algorithm imaging group was weak, the degree of pain relief was high after treatment, and the difference between the artificial intelligence algorithm imaging group and control group was statistically considerable ( < 0.005). Through grouped experiments, it was found that the construction of the AI imaging model was effective for the detection and recognition of lower extremity vein lesions in ultrasound images. To sum up, the ultrasound image evaluation and analysis using AI algorithm during MTS treatment was accurate and efficient, which laid a good foundation for future research, diagnosis, and treatment.
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http://dx.doi.org/10.1155/2021/5755671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321720PMC
July 2021

Variants and Genotype-Phenotype Features in Chinese CADASIL Patients.

Front Genet 2021 15;12:705284. Epub 2021 Jul 15.

Department of Geriatric Neurology, Xiangya Hospital, Central South University, Changsha, China.

Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a cerebral small vessel disease caused by mutations in the gene. Archetypal disease-causing mutations are cysteine-affecting variants within the 34 epidermal growth factor-like repeat (EGFr) region of the Notch3 extracellular subunit. Cysteine-sparing variants and variants outside the EGFr coding region associated with CADASIL phenotype have been reported. However, the linkage between untypical variants and CADASIL is unclear. In this study, we investigated the spectrum of variants in a cohort of 38 probands from unrelated families diagnosed as CADASIL. All coding exons of the gene were analyzed, and clinical data were retrospectively studied. We identified 23 different variants including 14 cysteine-affecting pathogenic variants, five cysteine-sparing pathogenic variants, two reported cysteine-sparing variants of unknown significance (VUS), and two novel VUS outside EGFr region. In retrospective studies of clinical data, we found that patients carrying cysteine-sparing pathogenic variants showed later symptom onset (51.36 ± 7.06 vs. 44.96 ± 8.82, = 0.023) and milder temporal lobe involvement (1.50 ± 1.74 vs. 3.11 ± 2.32, = 0.027) than patients carrying cysteine-affecting pathogenic variants. Our findings suggested that untypical variants comprise a significant part of variants and may be associated with a distinctive phenotype.
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http://dx.doi.org/10.3389/fgene.2021.705284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320595PMC
July 2021

Expression of Rad51 and the histo-morphological evaluation of testis of the sterile male cattle-yak.

Theriogenology 2021 Jul 9;172:239-254. Epub 2021 Jul 9.

Laboratory of Animal Anatomy & Tissue Embryology, Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.

Meiotic recombination is key to the repair of DNA double-strand break damage, provide a link between homologs for proper chromosome segregation as well as ensure genetic diversity in organisms. Defects in recombination often lead to sterility. The ubiquitously expressed Rad51 and the meiosis-specific DMC1 are two closely related recombinases that catalyze the key strand invasion and exchange step of meiotic recombination. This study cloned and sequenced the coding region of cattle-yak Rad51 and determined its mRNA and protein expression levels, evaluated its molecular and evolutionary relationship as well as evaluated the histo-morphological structure of testes in the yellow cattle, yak and the sterile cattle-yak hybrid. The Rad51 gene was amplified using PCR, cloned and sequenced using testicular cDNA from yak and cattle-yak. Real-time PCR was used to examine the expression levels of Rad51/DMC1 mRNA in the cattle, yak and cattle-yak testis while western blotting, immunofluorescence and immunohistochemistry were used to assess the protein expression and localization of Rad51/DMC1 protein in the testicular tissue sections. The results revealed that the mRNA and protein expression of Rad51 and DMC1 are extremely low in the male cattle-yak testis with a corresponding higher incidence of germ cell apoptosis. There was also thinning of the germinal epithelium possibly due to the depletion of the germ cells leading to the widening of the lumen area of the cattle-yak seminiferous tubule. Our findings provide support for the hypothesis that the low expression of Rad51 and DMC1 may contribute to the male hybrid sterility in the cattle-yak.
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http://dx.doi.org/10.1016/j.theriogenology.2021.06.018DOI Listing
July 2021

Mechanistic understanding of interspecific interaction between a C4 grass and a C3 legume via arbuscular mycorrhizal fungi as influenced by soil phosphorus availability using a C and N dual-labelled organic patch.

Plant J 2021 Jul 22. Epub 2021 Jul 22.

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, 712100, PR China.

Arbuscular mycorrhizal fungi (AMF) can improve plant nutrient acquisition either by directly supplying nutrients to plants or by promoting soil organic matter mineralisation, thereby affecting interspecific plant relationships in natural communities. We examined the mechanism by which P addition affects interspecific interactions between a C4 grass (Bothriochloa ischaemum, a dominant species in natural grasslands) and C3 legume (Lespedeza davurica, a subordinate species in natural grasslands) via AMF for plant growth by continuous C and N labelling combined with soil enzyme analyses. The results of N labelling revealed that P addition differently affected shoot N uptake via AMF by B. ischaemum and L. davurica. Specifically, P addition significantly increased shoot N uptake via AMF by B. ischaemum, but significantly decreased that by L. davurica. Interspecific plant interactions via AMF significantly facilitated plant N uptake via AMF by B. ischaemum but significantly inhibited that by L. davurica under soil P-limited conditions, while the opposite effect was observed in case of excess P addition. This was consistent with the impact of interspecific plant interaction via AMF on arbuscular mycorrhizal (AM) benefit for plant growth. Our data indicated that the capability of plant N uptake via AMF is an important mechanism which influences interspecific relationships between C4 grasses and C3 legumes. Moreover, the effect of AMF on the activities of soil enzymes responsible for N and P mineralization substantially contributed to the consequence of interspecific plant interaction via AMF for plant growth.
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http://dx.doi.org/10.1111/tpj.15434DOI Listing
July 2021

The InDel variants of sheep gene are associated with growth traits.

Anim Biotechnol 2021 Jul 13:1-9. Epub 2021 Jul 13.

College of Animal Science and Technology, Northwest A&F University, Yangling, P. R. China.

Insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) plays positive roles in the growth, proliferation of cells and early embryos development by binding mRNA targets. Recently, it had been shown that some polymorphic loci within gene were associated with growth traits in animals, especially in goats. Therefore, it has been hypothesized that some variants within gene may be also involved in growth traits of sheep. Nine insertion/deletion (InDel) mutations within were identified and three loci were polymorphic. Meanwhile, the association analyses between three InDels and growth traits were carried out in 745 sheep. The results showed that all InDels included 5 bp InDel in downstream region, 9 bp InDel in intron 4 and 15 bp InDel in intron 2 within were significantly associated with growth traits (<.05). Furthermore, at 5 and 9 bp InDel loci, the individuals of heterozygous genotype (ID) had superior growing performance especially at body weight (BW). In all, three InDels were crucial variants correlated with growth traits and could be applied in marker-assisted selection (MAS) in sheep.
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http://dx.doi.org/10.1080/10495398.2021.1942029DOI Listing
July 2021

YAP Accelerates Notch Driven Cholangiocarcinogenesis via mTORC1 in Mice.

Am J Pathol 2021 Jun 12. Epub 2021 Jun 12.

Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California; School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China. Electronic address:

Intrahepatic cholangiocarcinoma (iCCA) is a lethal malignant neoplasm with limited therapeutic options. Previous studies have found that Notch1 overexpression alone suffices to induce iCCA in the mouse, albeit after long latency. This study found that activation of the Yes-associated protein (Yap) proto-oncogene occurs during Notch1-driven iCCA progression. After co-expressing activated Notch1 intracellular domain (Nicd) and Yap (YapS127A) in the mouse liver, rapid iCCA formation and progression occurred in Nicd/Yap mice. Mechanistically, an increased expression of amino acid transporters and activation of the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway was detected in Nicd/Yap mouse liver tumors. Significantly, the genetic deletion of Raptor, the major mTORC1 component, completely suppressed iCCA development in Nicd/Yap mice. Elevated expression of Notch1, YAP, amino acid transporters, and members of the mTORC1 pathway was also detected ubiquitously in a collection of human iCCA specimens. Their levels were associated with a poor patient outcome. This study demonstrates that Notch and YAP concomitant activation is frequent in human cholangiocarcinogenesis. Notch and YAP synergize to promote iCCA formation by activating the mTORC1 pathway.
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http://dx.doi.org/10.1016/j.ajpath.2021.05.017DOI Listing
June 2021

Serum pepsinogen levels in different regions of China and its influencing factors: a multicenter cross-sectional study.

BMC Gastroenterol 2021 Jun 12;21(1):264. Epub 2021 Jun 12.

Department of General Practice/Health Management Center, The Second Affiliated Hospital of Zhejiang University, School of Medicine, No. 88 Jiefang Road, Hangzhou, China.

Background: The aim of this study is to investigate the difference of serum pepsinogen (PG) baseline levels in different regions of China and its influencing factors.

Methods: From October 2016 to October 2018, asymptomatic health checkup people who underwent nasal endoscopy in nine health management centers in different regions of China were collected. Lifestyle questionnaires were conducted, and serum PG and gastroscopy were performed. The differences in PG levels in baseline population (OLGA-0 grade) were studied according to geographical subregions of China. SPSS software was used for statistical analysis.

Results: 1922 patients were included in the final analysis. Compared with the non-atrophy (OLGA-0) group, PGR levels in atrophy group (OLGA-I to IV) were significantly decreased with the atrophy degree (p < 0.05). A total of 1590 baseline people (OLGA-0) were included in the study, including 254 from South China, 574 from East China, 210 from Southwest China, 332 from Northeast China, and 220 from Central/Northern China. There were significant differences in baseline PGI levels among the five regions (p < 0.05). The PGII levels were also different among the five regions, except for Central/Northern versus Southern China. PGR (PGI/PGII ratio) levels in Southern China were higher than other four regions. Further studies were conducted on the related factors that might affect the baseline PG level, which was affected by nationality, dietary habits, smoking, Helicobacter pylori infection and other related factors.

Conclusion: Influenced by many factors, the baseline PG levels are different in different regions of China. In the follow-up studies of PG cut-off value, different PG cut-off value based on region may be more effective in the screening of gastric cancer and precancerous lesions in China.
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http://dx.doi.org/10.1186/s12876-021-01794-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199540PMC
June 2021

Premarital Fertility and Marital Timing in Malawi.

Stud Fam Plann 2021 Jun 9;52(2):195-216. Epub 2021 Jun 9.

In Malawi, Africa, the median age at first marriage is among the lowest on the continent and adolescent fertility rates are among the highest. Using high-frequency panel data from the country designed to follow single women and men into marriage, we examine the extent to which premarital fertility is associated with the timing of marriage. Two notable findings emerge. First, premarital fertility typically leads to a more rapid transition into marriage, compared to not having had a premarital conception or birth, and this effect is as strong for men as it is for women. Second, among women with premarital fertility, those who are wealthier, and those who have two parents alive, have lower odds of not marrying. Among men with premarital fertility, however, no patterns predict their subsequent marital outcomes. This study contributes to the literature on fertility and marriage in sub-Saharan Africa by including men in the analysis.
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http://dx.doi.org/10.1111/sifp.12158DOI Listing
June 2021

Monocarboxylate Transporter 4 Triggered Cell Pyroptosis to Aggravate Intestinal Inflammation in Inflammatory Bowel Disease.

Front Immunol 2021 19;12:644862. Epub 2021 May 19.

Department of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

NLRP3 inflammasome has emerged as a crucial regulator of inflammatory bowel disease (IBD) characterized by a chronic inflammatory disease of the gastrointestinal tract. The expression of MCT4 is significantly increased in intestinal mucosal tissue of IBD, which has been identified to regulate intestinal barrier function. However, the function of MCT4 in cell pyroptosis remained unknown. In this study, we have established a stable cell line with MCT4 overexpression in HT-29 and CaCO2 cells, respectively. Functional analysis revealed that ectopic expression of MCT4 in CaCO2 cells contributed to cell pyroptosis as evidenced by LDH assay, which is largely attributed to Caspase-1-mediated canonical pyroptosis, but not Caspase-4 and Caspase-5, leading to cleave pro-IL-1β and IL-18 into mature form and release mediated by cleaved GSDMD. Mechanically, MCT4 overexpression in HT-29 and CaCO2 cell triggered the phosphorylation of ERK1/2 and NF-B p65, while inhibition of MCT4 by MCT inhibitor -Cyano-4-hydroxycinnamic acid (-CHCA) in HT-29 and CaCO2 cells led to a significant downregulation of ERK1/2 and NF-B activity. What's more, blockade of ERK1/2-NF-B pathway could reverse the promotion effect of MCT4 on IL-1β expression. Importantly, both MCT4 and Caspase-1, GSDMD were significantly increased in patients with IBD, and a positive clinical correlation between MCT4 and Caspase-1 expression was observed (p < 0.001). Taken together, these findings suggested that MCT4 promoted Caspase-1-mediated canonical cell pyroptosis to aggravate intestinal inflammation in intestinal epithelial cells (IECs) through the ERK1/2-NF-B pathway.
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http://dx.doi.org/10.3389/fimmu.2021.644862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170300PMC
May 2021

A 7-nt nucleotide sequence variant within the sheep gene affects female reproduction traits.

Anim Biotechnol 2021 Jun 3:1-7. Epub 2021 Jun 3.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

Lysine demethylase 3B () gene is a histone demethylase, demonstrating specific demethylation of the histone H3 lysine 9. It was detected as a sheep reproductive candidate gene by genome-wide scans, and related studies also showed its significance in female reproductive process. However, rare study researched its polymorphism. Herein, we hypothesized that the polymorphisms of gene were associated with sheep reproduction traits. A 7-nt nucleotide sequence variant (rs1088697156) within gene was identified in a total of 888 individuals, including the Australian White (AUW) sheep and Lanzhou Fat-tailed (LFT) sheep. II (insertion/insertion) and ID (insertion/deletion) genotypes of 7-nt variant were detected, which were at Hardy-Weinberg equilibrium (HWE) in detected breeds. Association analysis illustrated the 7-nt variant was significantly associated with the litter size, duration of pregnancy, live lamb number, live lamb rate, stillbirth number, stillbirth rate of average and different parity ( < 0.05) in AUW sheep. Moreover, 'ID' was the dominant genotype with excellent consistency in reproductive traits. It is instrumental to select individuals with ID genotype for improving the sheep reproduction traits. These findings suggest that the 7-nt variant within gene can be used as a candidate marker of reproduction traits for sheep breeding improvement by marker-assisted selection.
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http://dx.doi.org/10.1080/10495398.2021.1929270DOI Listing
June 2021

TRIP13 modulates protein deubiquitination and accelerates tumor development and progression of B cell malignancies.

J Clin Invest 2021 Jul;131(14)

Myeloma Center, Winthrop P. Rockefeller Institute, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.

Multiple myeloma (MM), a terminally differentiated B cell malignancy, remains difficult to cure. Understanding the molecular mechanisms underlying the progression of MM may identify therapeutic targets and lead to a fundamental shift in treatment of the disease. Deubiquitination, like ubiquitination, is a highly regulated process, implicated in almost every cellular process. Multiple deubiquitinating enzymes (DUBs) have been identified, but their regulation is poorly defined. Here, we determined that TRIP13 increases cellular deubiquitination. Overexpression of TRIP13 in mice and cultured cells resulted in excess cellular deubiquitination by enhancing the association of the DUB USP7 with its substrates. We show that TRIP13 is an oncogenic protein because it accelerates B cell tumor development in transgenic mice. TRIP13-induced resistance to proteasome inhibition can be overcome by a USP7 inhibitor in vitro and in vivo. These findings suggest that TRIP13 expression plays a critical role in B cell lymphoma and MM by regulating deubiquitination of critical oncogenic (NEK2) and tumor suppressor (PTEN, p53) proteins. High TRIP13 identifies a high-risk patient group amenable to adjuvant anti-USP7 therapy.
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http://dx.doi.org/10.1172/JCI146893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279583PMC
July 2021

Focal adhesion kinase (FAK) promotes cholangiocarcinoma development and progression via YAP activation.

J Hepatol 2021 May 28. Epub 2021 May 28.

Institute of Pathology, University of Regensburg, Regensburg, Germany. Electronic address:

Background & Aims: Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that is upregulated in many tumor types and is a promising target for cancer therapy. Herein, we elucidated the functional role of FAK in intrahepatic cholangiocarcinoma (iCCA) development and progression.

Methods: Expression levels and activation status of FAK were determined in human iCCA samples. The functional contribution of FAK to Akt/YAP murine iCCA initiation and progression was investigated using conditional Fak knockout mice and constitutive Cre or inducible Cre mice, respectively. The oncogenic potential of FAK was further examined via overexpression of FAK in mice. In vitro cell line studies and in vivo drug treatment were applied to address the therapeutic potential of targeting FAK for iCCA treatment.

Results: FAK was ubiquitously upregulated and activated in iCCA lesions. Ablation of FAK strongly delayed Akt/YAP-driven mouse iCCA initiation. FAK overexpression synergized with activated AKT to promote iCCA development and accelerated Akt/Jag1-driven cholangiocarcinogenesis. Mechanistically, FAK was required for YAP(Y357) phosphorylation, supporting the role of FAK as a central YAP regulator in iCCA. Significantly, ablation of FAK after Akt/YAP-dependent iCCA formation strongly suppressed tumor progression in mice. Furthermore, a remarkable iCCA growth reduction was achieved when a FAK inhibitor and palbociclib, a CDK4/6 inhibitor, were administered simultaneously in human iCCA cell lines and Akt/YAP mice.

Conclusions: FAK activation contributes to the initiation and progression of iCCA by inducing the YAP proto-oncogene. Targeting FAK, either alone or in combination with anti-CDK4/6 inhibitors, may be an effective strategy for iCCA treatment.

Lay Summary: We found that the protein FAK (focal adhesion kinase) is upregulated and activated in human and mouse intrahepatic cholangiocarcinoma samples. FAK promotes intrahepatic cholangiocarcinoma development, whereas deletion of FAK strongly suppresses its initiation and progression. Combined FAK and CDK4/6 inhibitor treatment had a strong anti-cancer effect in in vitro and in vivo models. This combination therapy might represent a valuable and novel treatment against human intrahepatic cholangiocarcinoma.
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http://dx.doi.org/10.1016/j.jhep.2021.05.018DOI Listing
May 2021

Positive Crosstalk Between Hedgehog and NF-κB Pathways Is Dependent on KRAS Mutation in Pancreatic Ductal Adenocarcinoma.

Front Oncol 2021 11;11:652283. Epub 2021 May 11.

Department of Gastroenterology, Kunshan Affiliated Hospital of Nanjing University of Chinese Medicine, Suzhou, China.

It has been shown that aberrant activation of the Hedgehog (Hh) and nuclear factor-kappa B (NF-κB) signaling pathways plays an important role in the pancreatic carcinogenesis, and KRAS mutation is a hallmark of pancreatic ductal adenocarcinoma (PDAC). Until now, the role of KRAS mutation in the context of crosstalk between Hh and NF-κB signaling pathways in PDAC has not been investigated. This study was to determine whether the crosstalk between the Hh and NF-κB pathways is dependent on KRAS mutation in PDAC. The correlation between Gli1, Shh, NF-κB p65 expression and KRAS mutation in PDAC tissues was firstly examined by immunohistochemistry. Next, Western blotting, qPCR, and immunofluorescence were conducted to examine the biological effects of interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) as NF-κB signaling agonists, Shh as an Hh ligand alone or in combination with KRAS small interfering RNA (si-KRAS) in KRAS-mutant PDAC cells (MT-KRAS; SW1990 and Panc-1), wild-type KRAS PDAC cells (WT-KRAS; BxPC-3) and mutant KRAS knock-in BxPC-3 cells as well as tumor growth . KRAS mutation-dependent crosstalk between Hh and NF-κB in PDAC cells was further assessed by Ras activity and luciferase reporter assays. The aberrant Hh and NF-κB pathway activation was found in PDAC tissues with KRAS mutation. The same findings were confirmed in MT-KRAS PDAC cells and MT-KRAS knock-in BxPC-3 cells, whereas this activation was not observed in WT-KRAS PDAC cells. However, the activation was significantly down-regulated by KRAS silencing in MT-KRAS PDAC cells. Furthermore, MT-KRAS cancer cell proliferation and survival and tumor growth after inoculation with MT-KRAS cells were promoted by NF-κB and Hh signaling activation. The pivotal factor for co-activation of NF-κB and Hh signaling is MT-KRAS protein upregulation, showing that positive crosstalk between Hh and NF-κB pathways is dependent upon KRAS mutation in PDAC.
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http://dx.doi.org/10.3389/fonc.2021.652283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144522PMC
May 2021

Polyethylene glycol combined with linaclotide is an effective and well-tolerated bowel preparation regimen for colonoscopy: an endoscopist-blinded, randomized, controlled trial.

Eur J Gastroenterol Hepatol 2021 May 21. Epub 2021 May 21.

Shandong University Cheeloo College of Medicine Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Gastroenterology Shandong Academy of Clinical Medicine, Provincial Hospital Affiliated to Shandong First Medical University, Jinan, People's Republic of China.

Background And Aim: Bowel preparation is an important determinant of the quality of colonoscopy. The traditional split-dose regimen of 4 L polyethylene glycol (PEG) solutions for bowel preparation is effective but poorly tolerated. The aim of this was to study the efficacy and tolerability of using linaclotide as an adjunctive agent with low-volume PEG for bowel preparation.

Methods: This was an endoscopist-blinded, randomized, controlled trial of 432 patients randomly assigned to three groups: 2 L PEG, 4 L PEG and 2 L PEG + 290 µg linaclotide (2 L PEG + L group). The primary outcome measure was efficacy of bowel preparation according to the Boston Bowel Preparation Scale (BBPS), with secondary outcomes of patients' tolerance, defecating frequency, complications, sleeping quality, cecal intubation rate, preparation-to-colonoscopy interval, withdrawal time, cecal intubation time, and adenoma and polyp detection rates.

Results: The percentage of adequate bowel preparation in the 2 L PEG + L group was higher than that of the 2 L PEG group (87.9% vs. 77.0%; P = 0.017), but not the 4 L PEG group (87.9% vs. 91.4%; P = 0.339). In terms of the mean (SD) BBPS score for the total and segmental colons, the bowel cleansing efficacy of 2 L PEG + L was superior to that of 2 L PEG and similar to that of 4 L PEG. Patient's tolerance (including complications, willingness to repeat and sleeping quality) were compatible between the 2 L and 2 L + L group, and the 4 L group was the worst among these three groups.

Conclusion: Two liters of PEG combined with 290 µg linaclotide was an effective and well-tolerated bowel preparation regimen.
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http://dx.doi.org/10.1097/MEG.0000000000002184DOI Listing
May 2021

Does laparoscopic hepatectomy offer benefits for patients with COPD? A propensity score analysis.

HPB (Oxford) 2021 Apr 27. Epub 2021 Apr 27.

Department of Liver Surgery, Center of Liver Transplantation, West China Hospital of Sichuan University, Chengdu, Sichuan Province, China. Electronic address:

Background: To date, it remains unclear whether laparoscopic hepatectomy (LH) is safe and feasible for patients with chronic obstructive pulmonary disease (COPD). Thus, we compared the perioperative outcomes of LH versus open hepatectomy (OH) in this special cohort of patients.

Methods: Between February 2014 and October 2020, 162 patients who underwent hepatectomy met the inclusion and exclusion criteria of this study. Perioperative data were compared between the two groups by propensity score matching (PSM) analysis.

Results: After PSM, 55 patients with well-balanced baseline data were included in each group. Intraoperative blood loss, overall postoperative complications, and postoperative pulmonary complications (PPCs) were significantly lower in the LH group than in the OH group (P < 0.001, P = 0.047, and P = 0.020 after PSM, respectively). However, major complications, early readmission, and early mortality were comparable between the two groups. According to multivariate analysis, high stage of COPD, preoperative tobacco use, and long operative time were independent risk factors for PPCs, whereas treatment with LH was a protective factor.

Conclusion: LH is safe and feasible for selected patients with COPD when performed by experienced surgeons, and it has superior perioperative outcomes (especially regarding PPCs) when compared to OH.
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http://dx.doi.org/10.1016/j.hpb.2021.04.007DOI Listing
April 2021

Integration of electrotaxis and durotaxis in cancer cells: Subtle nonlinear responses to electromechanical coupling cues.

Biosens Bioelectron 2021 Apr 30;186:113289. Epub 2021 Apr 30.

Department of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing, 100871, China; Beijing Innovation Center for Engineering Science and Advanced Technology, Peking University, Beijing, 100871, China. Electronic address:

Cells in living organisms live in multiphysics-coupled environments. There is growing evidence indicating that both exogenous electric field (EEF) and extracellular stiffness gradient (ESG) can regulate directional movement of cells, which are known as electrotaxis and durotaxis, respectively. How single cells respond to the ubiquitous electromechanical coupling cues, however, remains mysterious. Using microfluidic chip-based methodology and finite element-based electromechanical coupling design strategies, we develope an electromechanical coupling microchip system, enabling us to quantitatively investigate polarization and directional migration governed by EEF and ESG at the single cell level. It is revealed that both of electrotaxis and durotaxis nonlinearly depend on the physiological EEF and ESG, respectively. Specific combinations of EEF and ESG can subtly modify the polarization states of single cells and thus induce hyperpolarization and depolarization. Cells can integrate electrotaxis and durotaxis in response to multi-cue microenvironments via subtle mechanisms involving cooperation and competition during cellular electrosensing and mechanosensing. The work offers a platform for quantifying migration and polarization of cells driven by electromechanical cues, which is essential not only for elucidating physiological and pathological processes like embryo development, and invasion and metastasis of cancer cells, but for manipulating cell behaviors in a controllable and programmable fashion.
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http://dx.doi.org/10.1016/j.bios.2021.113289DOI Listing
April 2021

A 24-bp indel within the sheep gene is associated with litter size.

Anim Biotechnol 2021 May 4:1-6. Epub 2021 May 4.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.

Aryl Hydrocarbon Receptor (AHR) is a member of the PER-ARNT-SIM (PAS) family, which could mediate various biological processes, for instance, the balance of the immune system, cell proliferation, differentiation, vascular tissue remodeling and reproduction ability regulation. A previous research showed that the gene exerted important functions on the pig reproduction, implying that it could serve as a candidate gene related to animal reproductive traits. Here, the aim of this work was to identify potential insertion/deletion (indel) mutations of the gene in three sheep breeds and analyze the associations between these mutations and reproductive traits. Results showed that a 24-bp indel was uncovered three genotypes (II, ID and DD) in the Australian White sheep (AuW) and Lanzhou fat-tail sheep (LZFT) population, while there were only two genotypes (ID and DD) in Luxi black-headed sheep (LXBH). Moreover, the Fisher's exact test showed that the 24-bp indel mutation was significantly associated with litter size and live litter size in AuW sheep (Fisher's  < 0.05). Therefore, the 24-bp indel of sheep gene can contribute to sheep marker-assisted selection breeding and further improve the sheep reproductive performance.
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http://dx.doi.org/10.1080/10495398.2021.1914071DOI Listing
May 2021

Impact of Fertilizer on Crop Yield and C:N:P Stoichiometry in Arid and Semi-Arid Soil.

Int J Environ Res Public Health 2021 04 20;18(8). Epub 2021 Apr 20.

State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, China.

Fertilization can significantly affect the quality of crop and soil. To determine the effects of long-term fertilization on crop yield and carbon:nitrogen:phosphorus (C:N:P) stoichiometry in soil, a study was conducted on the terraced fields of the Loess Plateau from 2007 to 2019. Nine fertilization treatments were included: no fertilizer; organic fertilizer (O); organic and nitrogen fertilizers (ON); organic, nitrogen, and phosphorus fertilizers (ONP); organic and phosphorus fertilizers (OP); phosphorus and nitrogen fertilizers; potash and nitrogen fertilizers; potash, nitrogen, and phosphorus fertilizers; and potash and phosphorus fertilizers. Under these treatments except for CK and PK, crop yields initially decreased but later increased. The nutrient content and C:N:P stoichiometry increased in soil depth of 0-20 cm. The soil available nutrients did not change significantly with the duration of fertilization. The O, ON, ONP, and OP had the most evident effect on the enhancement of soil nutrient content, whereas O and ON had the most evident effect on the increase in soil organic carbon (SOC):total phosphorus (TP) and total nitrogen (TN):TP. In soil depth of 0-20 cm, crop yield, SOC:TN, SOC:TN, SOC:TP, and TN:TP significantly correlated with soil nutrients. This study indicated that long-term fertilization can effectively improve crop yield, soil fertility, and soil C:N:P stoichiometry. Meanwhile, the single application of an organic fertilizer or the combination of organic and nitrogen fertilizers can improve the condition of nitrogen limitation in arid and semi-arid areas.
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http://dx.doi.org/10.3390/ijerph18084341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072689PMC
April 2021

Comparative Analysis of Morphology, Photosynthetic Physiology, and Transcriptome Between Diploid and Tetraploid Barley Derived From Microspore Culture.

Front Plant Sci 2021 26;12:626916. Epub 2021 Feb 26.

Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Polyploids play an important role in the breeding of plant for superior characteristics, and many reports have focused on the effects upon photosynthesis from polyploidization in some plant species recently, yet surprisingly little of this is known for barley. In this study, homozygous diploid and tetraploid plants, derived from microspore culturing of the barley cultivar "H30," were used to assess differences between them in their cellular, photosynthetic, and transcriptomic characteristics. Our results showed that tetraploid barley has the distinct characteristics of polyploids, namely thicker and heavier leaves, enlarged stomata size or stomatal guard cell size, and more photosynthetic pigments and improved photosynthesis (especially under high light intensity). This enhanced photosynthesis of tetraploid barley was confirmed by several photosynthetic parameters, including net photosynthetic rate (P), stomatal conductance (G), intercellular CO concentration (C), transpiration rate (T), maximum net photosynthetic rate (P), light saturation point (LSP), maximum RuBP saturated rate carboxylation (V), and maximum rate of electron transport (J). Transcriptomic analyses revealed that just ~2.3% of all detected genes exhibited differential expression patterns [i.e., differentially expressed genes (DEGs)], and that most of these - 580 of 793 DEGs in total - were upregulated in the tetraploid barley. The follow-up KEGG analysis indicated that the most enriched pathway was related to photosynthesis-antenna proteins, while the downregulation of DEGs was related mainly to the light-harvesting cholorophyII /-binding protein (Lhcb1) component, both validated by quantitative PCR (qPCR). Taken together, our integrated analysis of morphology, photosynthetic physiology, and transcriptome provides evidences for understanding of how polyploidization enhances the photosynthetic capacity in tetraploids of barley.
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http://dx.doi.org/10.3389/fpls.2021.626916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970760PMC
February 2021

Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy.

Aging (Albany NY) 2021 03 19;13(7):9820-9837. Epub 2021 Mar 19.

Department of Orthopedic Surgery, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China.

The aim of this study was to determine the effect of HDAC6 inhibition using the selective inhibitor Tubastatin A (TubA) on the regulation of tert-butyl hydroperoxide (TBHP)-treated chondrocytes and a mouse OA model. Using conventional molecular biology methods, our results showed that the level of HDAC6 increases both in the cartilage of osteoarthritis (OA) mice and TBHP-treated chondrocytes . TubA treatment effectively inhibits the expression of HDAC6, attenuates oxidative stress, reduces the level of apoptotic proteins to maintain chondrocyte survival, and suppresses the extracellular matrix (ECM) degradation. In addition, our results also revealed that HDAC6 inhibition by TubA activates autophagy in chondrocytes, whereas the protective effects of TubA were abolished by autophagy inhibitor intervention. Subsequently, the positive effects of HDAC6 inhibition by TubA were also found in a mouse OA model. Therefore, our study provide evidence that HDAC6 inhibition prevents OA development, and HDAC6 could be applied as a potential therapeutic target for OA management.
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http://dx.doi.org/10.18632/aging.202736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064156PMC
March 2021

IRGM promotes melanoma cell survival through autophagy and is a promising prognostic biomarker for clinical application.

Mol Ther Oncolytics 2021 Mar 19;20:187-198. Epub 2020 Dec 19.

Department of Neurology and Center for Neuroinflammation and Experimental Therapeutics, University of Pennsylvania, Philadelphia, PA 19104, USA.

Previously, we showed that mouse immunity-related guanosine triphosphatase (GTPase) family M protein 1 (Irgm1) promotes malignant melanoma progression by inducing cellular autophagy flux and metastasis. Human IRGM, a truncated protein functionally distinct from its mouse counterpart, has several splice isoforms. In this study, we analyzed the association of IRGM and human melanoma clinical prognosis and investigated the function of IRGM in human melanoma cells. Data from the training cohort (n = 144) showed that overexpression of IRGM is proportional to melanoma genesis and clinical stages in human tissue chips. A validation cohort (n = 78) further confirmed that IRGM is an independent risk factor promoting melanoma progression and is associated with poor survival of patients. Among IRGM isoforms, we found that IRGMb is responsible for such correlation. In addition, IRGM promoted melanoma cell survival through autophagy, both and . We further showed that the blockade of translocation of high-mobility group box 1 (HMGB1) from the nucleus to cytoplasm inhibits IRGM1-mediated cellular autophagy and reduces cell survival. IRGM functions as a positive regulator of melanoma progression through autophagy and may serve as a promising prognostic marker and therapeutic target.
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http://dx.doi.org/10.1016/j.omto.2020.12.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889451PMC
March 2021

Genetic Analysis of Multiple Myeloma Identifies Cytogenetic Alterations Implicated in Disease Complexity and Progression.

Cancers (Basel) 2021 Jan 29;13(3). Epub 2021 Jan 29.

Myeloma Center, Department of Internal Medicine, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.

Multiple myeloma (MM) is a genetically heterogeneous disease characterized by genomic chaos making it difficult to distinguish driver from passenger mutations. In this study, we integrated data from whole genome gene expression profiling (GEP) microarrays and CytoScan HD high-resolution genomic arrays to integrate GEP with copy number variations (CNV) to more precisely define molecular alterations in MM important for disease initiation, progression and poor clinical outcome. We utilized gene expression arrays from 351 MM samples and CytoScan HD arrays from 97 MM samples to identify eight CNV events that represent possible MM drivers. By integrating GEP and CNV data we divided the MM into eight unique subgroups and demonstrated that patients within one of the eight distinct subgroups exhibited common and unique protein network signatures that can be utilized to identify new therapeutic interventions based on pathway dysregulation. Data also point to the central role of 1q gains and the upregulated expression of ANP32E, DTL, IFI16, UBE2Q1, and UBE2T as potential drivers of MM aggressiveness. The data presented here utilized a novel approach to identify potential driver CNV events in MM, the creation of an improved definition of the molecular basis of MM and the identification of potential new points of therapeutic intervention.
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http://dx.doi.org/10.3390/cancers13030517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866300PMC
January 2021

Women's Political Leadership and Adult Health: Evidence from Rural and Urban China.

J Health Soc Behav 2021 03 8;62(1):100-118. Epub 2021 Feb 8.

Brown University, Providence, RI, USA.

This study examined the role of women's political leadership at the community level in China, a context that has experienced recent political and socioeconomic change and has a distinctive rural-urban divide. Drawing on longitudinal data from the China Family Panel Studies (N range = 40,918-52,406 person-year observations), we found that female community directors outnumbered male directors in urban China but were much less common in rural areas. Female community directors had higher levels of human capital regardless of rural or urban location. Residents living in female-directed communities reported better mental health but not physical health or life satisfaction compared to those living in male-directed communities, and this association was most robust among rural women. For rural women, the mental health benefit of living in female-directed communities was partially explained by reduced personal experience of gender discrimination, suggesting that female leadership fosters ideational change toward women that lowers discriminatory behaviors among constituents.
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http://dx.doi.org/10.1177/0022146520987810DOI Listing
March 2021

High clinical heterogeneity in a Chinese pedigree of retinal vasculopathy with cerebral leukoencephalopathy and systemic manifestations (RVCL-S).

Orphanet J Rare Dis 2021 01 30;16(1):56. Epub 2021 Jan 30.

Department of Geriatric Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China.

Background: Being a newly defined disease, RVCL-S is underrecognized by clinicians globally. It is an autosomal dominantly inherited small vessel disease caused by the heterozygous C-terminal frameshift mutation in TREX1 gene. RVCL-S is featured by cerebral dysfunction, retinopathy, and vasculopathy in multiple internal organs. Misdiagnosis may cause devastating consequences in patients, such as iatrogenic PML caused by misuse of immunosuppressants. Thus, increasing awareness of this disease is in urgent need.

Results: We uncovered a large Chinese origin RVCL-S pedigree bearing the TREX1 mutation. A comprehensive characterization combining clinical, genetic, and neuropathological analysis was performed. The Intrafamilial comparison showed highly heterogeneous clinical phenotypes. Mutation carriers in our pedigree presented with retinopathy (8/13), seizures (2/13), increased intracranial pressure (1/13), mild cognitive impairment (3/13), stroke-like episode (3/13), mesenteric ischemia (1/13), nephropathy (9/13), ascites (3/13), hypertension (9/13), hyperlipidemia (3/8), hypoalbuminemia (3/8), normocytic anemia (3/8), subclinical hypothyroidism (1/8), hyperfibrinogenemia (1/8), hyperparathyroidism (2/8), and abnormal inflammatory markers (4/8). The constellation of symptoms is highly varied, making RVCL-S a challenging diagnosis. Comparison with reported RVCL-S pedigrees further revealed that the mesenteric ischemia is a novel clinical finding and the MRS pattern of brain lesions is emulating neoplasm and tumefactive demyelination.

Conclusion: Our reports characterize a highly heterogeneous RVCL-S pedigree, highlight the probability of misdiagnosis in clinical practice, and broaden the clinical spectrum of RVCL-S.
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http://dx.doi.org/10.1186/s13023-021-01712-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847589PMC
January 2021

An insertion/deletion within the CREB1 gene identified using the RNA-sequencing is associated with sheep body morphometric traits.

Gene 2021 Apr 21;775:145444. Epub 2021 Jan 21.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

In a previous study, the cyclic AMP response element-binding protein 1 (CREB1) gene, which is likely involved in the regulation of fat metabolism in sheep adipose tissue, was identified using RNA sequencing. CREB1 is a transcription factor that participates in the regulation of cell proliferation, differentiation, and survival as well as energy metabolism. Therefore, based on preliminary studies, this study aimed to reveal the correlation between the insertion/deletion (indel) polymorphism of the CREB1 gene and sheep growth traits. One insertion variation of the ovine CREB1 gene, C3-ins-26 bp, was investigated in 1847 Chinese and Mongolian sheep breeds. The minor allele frequencies in the CREB1 gene varied from 0.021 to 0.938. Further, statistical analyses indicated that the C3-ins-26 bp indel in the CREB1 gene was significantly related to various body measurements (body length, height, and index; chest width, depth, and width index; cannon circumference index; and height at the hip cross) in a Tan sheep population (p < 0.05). Collectively, these findings may provide important insights into marker-assisted selection of sheep.
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http://dx.doi.org/10.1016/j.gene.2021.145444DOI Listing
April 2021

Correlation of Hypoxia-inducible facto-1α and C-reactive protein with disease evaluation in patients with ulcerative colitis.

Am J Transl Res 2020 15;12(12):7826-7835. Epub 2020 Dec 15.

Department of Gastroenterology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University Ji'nan, Shandong Province, China.

Objective: To investigate the expression levels of hypoxia-inducible factor-1α (HIF-1α) and C-reactive protein (CRP) in patients with ulcerative colitis and correlations of HIF-1α and CRP levels with disease severity.

Methods: A total of 82 patients with confirmed ulcerative colitis were enrolled in this study and according to the disease severity grading, these patients were assigned into three groups: mild group (n=25), moderate group (n=31) and severe group (n=26). And other 30 patients without ulcerative colitis as demonstrated by colonoscopy examination were enrolled in control group in the same period. HIF-1α and CRP levels were detected by ELISA and Real-time PCR and compared among different groups. Pearson's correlation analysis was performed to evaluate the correlations of HIF-1α and CRP levels with disease severity. Logistic regression analysis was used to explore risk factors of disease severity in patients with ulcerative colitis.

Results: The expression levels of HIF-1α and CRP in ulcerative colitis group were significantly higher than those in control group (all P<0.001). The levels of HIF-1α and CRP in patients with ulcerative colitis increased remarkably with the increase of disease severity. Patients in mild group had the lowest levels of HIF-1α and CRP, while patients in severe group had the highest levels of HIF-1α and CRP. Logistic regression analysis showed that the expression of HIF-1α and CRP were the risk factors for disease severity of ulcerative colitis (all P<0.001). And Pearson correlation analysis showed that HIF-1α and CRP levels were significantly associated with Rachmilewitz score and disease activity index (DAI), respectively (all P<0.001).

Conclusion: The levels of HIF-1α and CRP were up-regulated in patients with ulcerative colitis and positively correlated with the progression of ulcerative colitis, indicating that the detection of HIF-1α and CRP expression could be used for predicting the disease severity.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791512PMC
December 2020

Effects of natural vegetation restoration on dissolved organic matter (DOM) biodegradability and its temperature sensitivity.

Water Res 2021 Mar 27;191:116792. Epub 2020 Dec 27.

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, PR China; Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry Water Resources, Yangling 712100, PR China. Electronic address:

Biodegradation of dissolved organic matter (DOM) plays a key role in regulating both production of greenhouse gases and accumulation and stabilisation of soil organic matter (SOM). However, the mechanisms by which natural vegetation restoration affects the extent, rate, and temperature sensitivity of DOM biodegradation are poorly understood. Elucidating these mechanisms is important for SOM management, especially in light of future climate warming scenarios. In this study, a laboratory DOM solution incubation experiment was conducted to comprehensively investigate the effects of temperature and natural vegetation restoration spanning a period of 160 y on DOM biodegradation in the Loess Plateau, China. The results indicated that dissolved organic C (DOC) biodegradation significantly decreased with vegetation restoration after an incubation period of 60 d. Further, biodegradation of dissolved organic N (DON) and dissolved organic P (DOP) significantly decreased after farmland abandonment. Specifically, the lowest values were observed in pioneer (Populus davidiana) and mingled (Populus davidiana and Quercus liaotungensis) forests. Generally, an increase in temperature significantly promoted the biodegradation of DOC, DON, and DOP by enhancing the microbial utilisation efficiencies of recalcitrant humic substrates (i.e., low-molecular-weight humic materials). Our results suggest that DOM biodegradability and its temperature sensitivity were regulated by DOM substrate quality (i.e, recalcitrant humic materials), and microbial properties (i.e., gram-negative bacterial and fungal PLFA, enzyme activities). Additionally, our results suggest that climax forest communities (Quercus liaotungensis) played a vital role in reducing DOC and DOP losses. This could be attributed to the low Q10 of the DOC and DOP biodegradation rates.
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http://dx.doi.org/10.1016/j.watres.2020.116792DOI Listing
March 2021

Propensity score matching analysis for outcomes of laparoscopic versus open caudate lobectomy.

ANZ J Surg 2021 04 30;91(4):E168-E173. Epub 2020 Dec 30.

Department of Liver Surgery, Center of Liver Transplantation, West China Hospital of Sichuan University, Chengdu, China.

Background: To date, laparoscopic caudate lobectomy (LCB) remains a challenge, and evidence about its value is rare. Therefore, this study was performed to assess the benefits of LCB for patients with tumours located in the caudate lobe compared with open caudate lobectomy (OCB).

Methods: From October 2015 to June 2019, 102 patients meeting the inclusion and exclusion criteria were eligible for this study. The patients were divided into LCB and OCB groups. Short-term outcomes between the groups were compared with propensity score matching (PSM).

Results: LCB was performed in 31 of the 102 included patients. After PSM, 30 patients with well-balanced baseline levels were enrolled in each group. Intraoperative blood loss in the LCB group was less than that in the OCB group (median, 100 versus 200 mL, P = 0.017), even though it did not reach a statistically significant difference after PSM (median, 100 versus 187.5 mL, P = 0.085). Moreover, although post-operative overall complications were similar in the two groups, a tendency of lower rates of respiratory infection in the LCB group than the OCB group was observed (0% versus 12.7%, P = 0.054 before PSM; 0% versus 16.7%, P = 0.063 after PSM). There was no early mortality in either group. LCB had a shorter post-operative hospital stay than OCB (median, 5 versus 6 days, P < 0.001). However, the hospitalization cost was higher in the LCB group than in the OCB group (P < 0.001).

Conclusion: LCB is safe and feasible in selected patients when performed by experienced surgeons.
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http://dx.doi.org/10.1111/ans.16512DOI Listing
April 2021

Plant-microbial feedback in secondary succession of semiarid grasslands.

Sci Total Environ 2021 Mar 6;760:143389. Epub 2020 Nov 6.

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China; Institute of Soil and Water Conservation, Chinese Academy of Sciences & Ministry of Water Resources, Yangling, Shaanxi 712100, People's Republic of China. Electronic address:

Plant-soil feedback (PSF) is an important driver of plant community dynamics. The role of plant species in PSF has been emphasized for secondary succession processes; however, microbial responses to PSF and the underlying mechanisms responsible for their effects on plant succession remain poorly understood, particularly in semiarid grassland ecosystems. Here, we conducted a greenhouse experiment using soil collected from early-, mid-, and late-successional plant communities to measure net pairwise PSF for species grown under monoculture. Soils conditioned by pre-successional species had a positive feedback effect on subsequent plant species, whereas soil conditioned by subsequent plant species had a negative feedback effect on pre-successional species. The feedback effect of plants from different successional stages on soil bacterial and fungal communities was mainly positive. However, the bacterial genera in the soil conditioned by early- and mid-successional species and fungal classes in the soil conditioned by early- successional species had a negative feedback effect on late-successional species. Thus, the effects of soil fungal and bacterial communities on species in other successional stages varied with taxonomic level. Our results provide insight into the manner in which soil microbial communities influence PSF responses during secondary succession processes.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143389DOI Listing
March 2021

Stylet Slow-pull Versus Standard Suction for Endoscopic Ultrasound-guided Fine-needle Aspiration/Biopsy of Pancreatic Solid Masses: A Meta-Analysis.

J Clin Gastroenterol 2021 02;55(2):103-109

Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

Background And Study Aim: There is limited evidence on the diagnostic performance of the stylet slow-pull (SP) method for endoscopic ultrasound-guided fine-needle aspiration/biopsy. The aim of this study was to compare the SP method with standard suction (SS) for endoscopic ultrasound-guided fine-needle aspiration/biopsy of solid pancreatic masses.

Methods: A computerized bibliographic search of the main databases, including PubMed, EMBASE, Cochrane Library, and Science Citation Index, was performed through February 2020. The main outcome measurements were diagnostic accuracy, cellularity, low blood contamination, adequate core tissue acquisition, and technical success rate.

Results: Eleven studies (including 6 randomized trials) were included, with a total of 504 patients who underwent SP and 551 who underwent SS. Diagnostic accuracy was significantly superior in the SP group, compared with the SS group [odds ratio (OR)=1.60; 95% confidence interval (CI), 1.14-2.26]. The SP group had higher pooled rates of low blood contamination (OR=1.93; 95% CI, 1.29-2.87) and adequate core tissue acquisition (OR=1.91; 95% CI, 1.11-3.26) than the SS group. There was no significant difference between groups in the adequacy of cellularity (OR=0.99; 95% CI, 0.63-1.57; P=0.98) or technical success rate (OR=0.38; 95% CI, 0.13-1.15; P=0.09).

Conclusions: The authors provide evidence that SP is superior to SS in diagnostic accuracy, low blood contamination, and adequate core tissue acquisition, without reducing adequacy of cellularity or technical success rate.
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http://dx.doi.org/10.1097/MCG.0000000000001408DOI Listing
February 2021
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