Publications by authors named "Hongwei Sun"

189 Publications

Association of serotonin transporter-linked polymorphic region (5-HTTLPR) with heat pain stimulation and postoperative pain in gastric cancer patients.

Mol Pain 2021 Jan-Dec;17:17448069211006606

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education, Beijing), Department of Anesthesiology, Peking University Cancer Hospital & Institute, Beijing, China.

Background: The aim of this study was to assess whether the genotype of the serotonin transporter-linked polymorphic region (5-HTTLPR) in gastric cancer patients is associated with postoperative pain and pain threshold.

Methods: We conducted a prospective cohort study of 251 patients scheduled for gastrectomy from May to September 2019. All patients enrolled in the study were asked to complete the Hospital Anxiety and Depression Scale questionnaire. Heat pain threshold (HPT), cold pain threshold (CPT) and Pressure pain threshold (PPT) were measured for all participants one day prior to surgery. Blood samples were collected for genetic testing. All patients were connected to a patient-controlled intravenous analgesia (PCIA) pump at the end of the surgery. After exclusion of 15 patients, the postoperative conditions of 236 patients were recorded.

Results: Distribution of homozygous long (L/L), heterozygous (L/S), and homozygous short (S/S) 5-HTTLPR genotypes among participants were 26 (11.0%), 91 (38.6%), and 119 (50.4%), respectively. Heat pain threshold ( = 0.038) and Numerical rating scale (NRS) in the 1st postoperative 24 h ( = 0.026) were significantly different between long (L/L) and short (S/S) genotype carriers.

Conclusions: In patients with gastric cancer, heat pain stimulation is associated with 5-HTTLPR polymorphism, and postoperative pain may be related to 5-HTTLPR polymorphism.
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http://dx.doi.org/10.1177/17448069211006606DOI Listing
April 2021

Development and Evaluation of Nomograms to Predict the Cancer-Specific Mortality and Overall Mortality of Patients with Hepatocellular Carcinoma.

Biomed Res Int 2021 29;2021:1658403. Epub 2021 Mar 29.

Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.

Hepatocellular carcinoma (HCC) is the most common type among primary liver cancers (PLC). With its poor prognosis and survival rate, it is necessary for HCC patients to have a long-term follow-up. We believe that there are currently no relevant reports or literature about nomograms for predicting the cancer-specific mortality of HCC patients. Therefore, the primary goal of this study was to develop and evaluate nomograms to predict cancer-specific mortality and overall mortality. Data of 45,158 cases of HCC patients were collected from the Surveillance, Epidemiology, and End Results (SEER) program database between 2004 and 2013, which were then utilized to develop the nomograms. Finally, the performance of the nomograms was evaluated by the concordance index (C-index) and the area under the time-dependent receiver operating characteristic (ROC) curve (td-AUC). The categories selected to develop a nomogram for predicting cancer-specific mortality included marriage, insurance, radiotherapy, surgery, distant metastasis, lymphatic metastasis, tumor size, grade, sex, and the American Joint Committee on Cancer (AJCC) stage; while the marriage, radiotherapy, surgery, AJCC stage, grade, race, sex, and age were selected to develop a nomogram for predicting overall mortality. The C-indices for predicted 1-, 3-, and 5-year cancer-specific mortality were 0.792, 0.776, and 0.774; the AUC values for 1-, 3-, and 5-year cancer-specific mortality were 0.830, 0.830, and 0.830. The C-indices for predicted 1-, 3-, and 5-year overall mortality were 0.770, 0.755, and 0.752; AUC values for predicted 1-, 3-, and 5-year overall mortality were 0.820, 0.820, and 0.830. The results showed that the nomograms possessed good agreement compared with the observed outcomes. It could provide clinicians with a personalized predicted risk of death information to evaluate the potential changes of the disease-specific condition so that clinicians can adjust therapy options when combined with the actual condition of the patient, which is beneficial to patients.
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http://dx.doi.org/10.1155/2021/1658403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024067PMC
March 2021

Asymptomatic epiploic appendage with torsion in laparoscopic surgery: a case report and literature review.

Ann Palliat Med 2021 Apr 1. Epub 2021 Apr 1.

Department of General Surgery, Strategic Support Force Medical Center, Beijing, China.

Torsion of an epiploic appendage may result in epiploic appendagitis, which is a rare cause of acute abdominal pain. However, no previous reports have described an asymptomatic twisted epiploic appendage found during laparoscopic surgery to the best of our knowledge. This case describes a 66-year-old man who was admitted to our medical center with yellowness of the skin and eyes that had lasted over two months. Physical examination showed slight yellow staining of the skin and sclera. Blood analysis indicated liver dysfunction and jaundice. Routine blood, C-reactive protein, and levels of tumor markers were normal. The contrast-enhanced abdominal and pelvic computed tomography, magnetic resonance imaging, and magnetic resonance cholangiopancreatography revealed gallbladder atrophy and choledocholithiasis. The patient underwent laparoscopic surgery for the removal of the choledocholithiasis. The laparoscopic exploration unexpectedly revealed a twisted and ischemic epiploic appendage, which was surgically removed. The postoperative pathological examination uncovered necrosis of adipocytes and vascular obstruction, but there was no inflammation of the epiploic appendage. The patient had a satisfactory recovery during the 16-month follow-up period. This case describes and provides a feasible management approach for an asymptomatic twisted epiploic appendage discovered during surgery and highlights its pathological characteristics, reflecting the early stage of epiploic appendagitis.
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http://dx.doi.org/10.21037/apm-20-2128DOI Listing
April 2021

Differences in Anthocyanin Accumulation Patterns and Related Gene Expression in Two Varieties of Red Pear.

Plants (Basel) 2021 Mar 25;10(4). Epub 2021 Mar 25.

Lab of Pear Genetic Improvement and Germplasm Innovation, Qingdao Agricultural University, Qingdao 266109, China.

Red pear is a popular fruit that is appreciated for its attractive and distinctive appearance and mild flavor. In this study, we investigated the mechanism underlying the red coloration of pear skin using the 'Xinqihong' cultivar-which was selected as a spontaneous bud sport mutant of the 'Xinli 7'( Rehd.) variety and has a stronger red color that is retained in the mature fruit-as an experimental model. We compared the phenotype and gene expression patterns of the two varieties and found no significant differences at the early stage of fruit development. However, although the red color of 'Xinli 7' fruits began to fade 107 days after full bloom, that of 'Xinqihong' fruits persisted until the time of harvest. Transcriptome sequencing identified 639 genes that were differentially expressed between the two varieties, including genes related to light, calcium, and hormone signaling (e.g., , , and the calmodulin related genes). Moreover, anthocyanin biosynthesis genes were downregulated as the red color of 'Xinli 7' fruits faded. These results provide insight into the molecular basis of color differences in red pears that can be useful for improving its fruit quality and commercial value.
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http://dx.doi.org/10.3390/plants10040626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066033PMC
March 2021

Development of highly efficient platinum catalysts for hydroalkoxylation and hydroamination of unactivated alkenes.

Nat Commun 2021 03 29;12(1):1953. Epub 2021 Mar 29.

Shenzhen Grubbs Institute and Department of Chemistry, Guangdong Provincial Key Laboratory of Catalysis, Southern University of Science and Technology, Shenzhen, Guangdong, China.

Hydrofunctionalization, the direct addition of an X-H (e.g., X=O, N) bond across an alkene, is a desirable strategy to make heterocycles that are important structural components of naturally occurring molecules. Described here is the design and discovery of "donor-acceptor"-type platinum catalysts that are highly effective in both hydroalkoxylation and hydroamination of unactivated alkenes over a broad range of substrates under mild conditions. A number of alkene substitution patterns are accommodated, including tri-substituted, 1,1-disubstituted, (E)-disubstituted, (Z)-disubstituted and even mono-substituted double bonds. Detailed mechanistic investigations suggest a plausible pathway that includes an unexpected dissociation/re-association of the electron-deficient ligand to form an alkene-bound "donor-acceptor"-type intermediate. These mechanistic studies help understand the origins of the high reactivity exhibited by the catalytic system, and provide a foundation for the rational design of chiral catalysts towards asymmetric hydrofunctionalization reactions.
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http://dx.doi.org/10.1038/s41467-021-22287-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007598PMC
March 2021

Effect of ketamine on mood dysfunction and spatial cognition deficits in PTSD mouse models via HCN1-BDNF signaling.

J Affect Disord 2021 05 4;286:248-258. Epub 2021 Mar 4.

School of Psychology, Weifang Medical University, 7166# Baotong West Street, Weifang, Shandong 261053, China. Electronic address:

Background: Post-traumatic stress disorder (PTSD) is a debilitating mental disease with high morbidity and major social and economic relevance. No efficient treatment for PTSD has thus far been identified. Clinical research has shown that ketamine can rapidly alleviate symptoms in patients with chronic PTSD; however, its pharmacological mechanism has yet to be determined.

Methods: This study aimed to identify a model of single prolonged stress (SPS), which induced PTSD-like features in adult mice. Once the model was established, stress-related behavioral changes in the mouse model were evaluated after intraperitoneal injection of ketamine (10 mg/kg). Alterations in certain proteins (HCN1, BDNF, and PSD95) and synaptic ultrastructure in the prefrontal cortex (PFC) and hippocampus (HIP) were measured.

Results: The mice under the SPS model exhibited anxiety- and depression-like behaviors and induced spatial cognitive deficits, accompanied by elevated HCN1 protein expression in the PFC and HIP, reduced brain-derived neurotrophic factor (BDNF) and PSD95 proteins, and alterations in synaptic morphology. After ketamine administration, the SPS-treated mice restored their protein levels and synaptic ultrastructure in the PFC, and their PTSD-like behaviors improved. However, learning and memory in the SPS-treated mice did not improve in the water maze test, and no significant changes in protein level and synaptic ultrastructure in the HIP were shown.

Limitations: The electrophysiological mechanism of the HCN1 ion channel after ketamine administration was not explored.

Conclusion: Ketamine could generally improve SPS-induced mood dysfunction in mice but exerted no effect on the spatial cognitive function, which could be related to the alterations in synaptic morphology and function mediated by HCN1-related BDNF signaling in the PFC and HIP.
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http://dx.doi.org/10.1016/j.jad.2021.02.058DOI Listing
May 2021

CD146 is a Novel ANGPTL2 Receptor that Promotes Obesity by Manipulating Lipid Metabolism and Energy Expenditure.

Adv Sci (Weinh) 2021 Mar 27;8(6):2004032. Epub 2021 Jan 27.

Key Laboratory of RNA Biology Key Laboratory of Protein and Peptide Pharmaceuticals Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China.

Obesity and its related complications pose an increasing threat to human health; however, targetable obesity-related membrane receptors are not yet elucidated. Here, the membrane receptor CD146 is demonstrated to play an essential role in obesity. In particular, CD146 acts as a new adipose receptor for angiopoietin-like protein 2 (ANGPTL2), which is thought to act on endothelial cells to activate adipose inflammation. ANGPTL2 binds to CD146 to activate cAMP response element-binding protein (CREB), which then upregulates CD146 during adipogenesis and adipose inflammation. CD146 is present in preadipocytes and mature adipocytes, where it is mediated by its ligands ANGPTL2 and galectin-1. In preadipocytes, CD146 ablation suppresses adipogenesis, whereas the loss of CD146 in mature adipocytes suppresses lipid accumulation and enhances energy expenditure. Moreover, anti-CD146 antibodies inhibit obesity by disrupting the interactions between CD146 and its ligands. Together, these findings demonstrate that ANGPTL2 directly affects adipocytes via CD146 to promote obesity, suggesting that CD146 can be a potential target for treating obesity.
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http://dx.doi.org/10.1002/advs.202004032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967059PMC
March 2021

A commentary on "The efficacy and safety of low dialysate sodium levels for patients with maintenance haemodialysis: A systematic review and meta-analysis" [Int J Surg. 2020; 79:332-339].

Int J Surg 2021 Apr 11;88:105917. Epub 2021 Mar 11.

Hemodialysis Center, Liaoyang Central Hospital, Liaoning, 111000, China. Electronic address:

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http://dx.doi.org/10.1016/j.ijsu.2021.105917DOI Listing
April 2021

PINK1/PARK2 dependent mitophagy effectively suppresses NLRP3 inflammasome to alleviate acute pancreatitis.

Free Radic Biol Med 2021 Apr 23;166:147-164. Epub 2021 Feb 23.

Translational Medicine Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, PR China. Electronic address:

Background: Acute pancreatitis (AP) is a clinically common acute inflammatory disease in digestive system, leading to systemic inflammatory response syndrome (SIRS) and severe acute pancreatitis (SAP). It was reported that PINK1/PARK2 dependent mitophagy played an important role in various inflammatory diseases. However, its role in AP has not been elucidated. Herein, we explore the effect of mitophagy in the pathogenesis of AP.

Methods: Firstly, we established cerulein-induced AP group and arginine-induced SAP group based on wild, PINK1 and PARK2 mice. Pancreatic samples were harvested for further investing the mitochondrial dynamics, mitophagy alterations, NLRP3 inflammatory pathway etc. Furthermore, peripheral blood mononuclear cells from SAP patients were collected to examine the expression of mitophagy-related indicators. Additionally, the interrelationship between mitophagy and NLRP3 inflammasome was also explored in AP.

Results: It was confirmed that mitochondria were damaged in both AP and SAP models. The expressions of PINK1, PARK2 and mitochondrial autophagosomes were elevated in wild AP group, which were decreased in SAP group over time. Similarly, the expressions of PINK1 and PAKR2 in peripheral blood mononuclear cells were significantly lower in SAP patients. Besides, in PINK1 and PARK2 mice AP groups, more pronounced inflammatory infiltration, increased apoptotic and necrotic levels and upregulated NLRP3 inflammasome pathway were detected. After injection with MCC950, NLRP3 inflammasome production was notably reduced in PINK1and PARK2mice, which effectively alleviated the pancreatic damage and inflammatory cell infiltration.

Conclusion: Our study suggested that mitochondrial dysfunction activated PINK1/PARK2-mediated mitophagy in AP, while mitophagy was impaired in SAP. PINK1 and PARK2 mice were more sensitive to onset of SAP and the deficiency of mitophagy could lead to the formation of NLRP3 inflammasome.
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http://dx.doi.org/10.1016/j.freeradbiomed.2021.02.019DOI Listing
April 2021

4-Hydroxyphenylpyruvate Dioxygenase-Like Protein Promotes Pancreatic Cancer Cell Progression and Is Associated With Glutamine-Mediated Redox Balance.

Front Oncol 2020 18;10:617190. Epub 2021 Jan 18.

Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China.

Tumor cells develop a series of metabolic reprogramming mechanisms to meet the metabolic needs for tumor progression. As metabolic hubs in cells, mitochondria play a significant role in this process, including energy production, biosynthesis, and redox hemostasis. In this study, we show that 4-hydroxyphenylpyruvate dioxygenase-like protein (HPDL), a previously uncharacterized protein, is positively associated with the development of pancreatic ductal adenocarcinoma (PDAC) and disease prognosis. We found that overexpression of HPDL in PDAC cells promotes tumorigenesis , whereas knockdown of HPDL inhibits cell proliferation and colony formation. Mechanistically, we found that HPDL is a mitochondrial intermembrane space localized protein that positively regulates mitochondrial bioenergetic processes and adenosine triphosphate (ATP) generation in a glutamine dependent manner. Our results further reveal that HPDL protects cells from oxidative stress by reprogramming the metabolic profile of PDAC cells toward glutamine metabolism. In short, we conclude that HPDL promotes PDAC likely through its effects on glutamine metabolism and redox balance.
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http://dx.doi.org/10.3389/fonc.2020.617190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848781PMC
January 2021

Data Efficient Reinforcement Learning for Integrated Lateral Planning and Control in Automated Parking System.

Sensors (Basel) 2020 Dec 18;20(24). Epub 2020 Dec 18.

School of Automotive Studies, Tongji University, Shanghai 201804, China.

Reinforcement learning (RL) is a promising direction in automated parking systems (APSs), as integrating planning and tracking control using RL can potentially maximize the overall performance. However, commonly used model-free RL requires many interactions to achieve acceptable performance, and model-based RL in APS cannot continuously learn. In this paper, a data-efficient RL method is constructed to learn from data by use of a model-based method. The proposed method uses a truncated Monte Carlo tree search to evaluate parking states and select moves. Two artificial neural networks are trained to provide the search probability of each tree branch and the final reward for each state using self-trained data. The data efficiency is enhanced by weighting exploration with parking trajectory returns, an adaptive exploration scheme, and experience augmentation with imaginary rollouts. Without human demonstrations, a novel training pipeline is also used to train the initial action guidance network and the state value network. Compared with path planning and path-following methods, the proposed integrated method can flexibly co-ordinate the longitudinal and lateral motion to park a smaller parking space in one maneuver. Its adaptability to changes in the vehicle model is verified by joint Carsim and MATLAB simulation, demonstrating that the algorithm converges within a few iterations. Finally, experiments using a real vehicle platform are used to further verify the effectiveness of the proposed method. Compared with obtaining rewards using simulation, the proposed method achieves a better final parking attitude and success rate.
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http://dx.doi.org/10.3390/s20247297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766926PMC
December 2020

Maternal Separation-Induced Histone Acetylation Correlates with BDNF-Programmed Synaptic Changes in an Animal Model of PTSD with Sex Differences.

Mol Neurobiol 2021 Apr 27;58(4):1738-1754. Epub 2020 Nov 27.

School of Psychology, Weifang Medical University, 7166# Baotong West Street, Weifang, 261053, Shandong, People's Republic of China.

Maternal separation (MS) causes long-lasting epigenetic changes in the brain and increases vulnerability to traumatic events in adulthood. Of interest, there may be sex-specific differences in these epigenetic changes. In this study, the extent of histone acetylation in the hippocampus (HIP) and the expression of BDNF were measured to determine whether BDNF influences risk of PTSD following MS in early life. Rat offspring were separated from their dams (3 h/day or 6 h/day from PND2~PND14). Then, pups were treated with a single prolonged stress (SPS) procedure when they reached adulthood (PND80). In animals stressed with the SPS procedure in adulthood, those that had increased MS intensity in childhood demonstrated more significant changes in performance on tests of anxiety, depression, and contextual fear memory. Reduced levels of total BDNF mRNA and protein were observed after SPS treatment and further declined in groups with greater MS time in childhood. Interestingly, these changes were correlated with decreased H3K9ac levels and increased HDAC2 levels. Additional MS also led to more severe ultrastructural synaptic damage in rats that experienced the SPS procedure, particularly in the CA1 and CA3 region of the HIP, reflecting impaired synaptic plasticity in these regions. Interestingly, male rats in the MS3h-PTSD group showed decreased anxiety, but no similar changes were found in female rats, suggesting a degree of gender specificity in coping with stress after mild MS. In summary, this study suggests that the epigenetic signatures of the BDNF genes can be linked to HIP responses to stress, providing insights that may be relevant for people at risk of stress-related psychopathologies.
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http://dx.doi.org/10.1007/s12035-020-02224-6DOI Listing
April 2021

Sexual Differences in Physiological and Transcriptional Responses to Salinity Stress of .

Front Plant Sci 2020 19;11:517962. Epub 2020 Oct 19.

The State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Hangzhou, China.

Willow (), a dioecious plant, is an important ornamental tree species in the world. , a perennial woody plant species naturally distributed on the Songnen Plain saline-alkali land in northeast China, has a high saline condition. To study the sexual differences of in salinity tolerance, the physiological and transcriptional responses to salinity were compared between female and male cuttings. Under salinity stress, the female leaves exhibited higher superoxide dismutase and peroxidase activities and photosynthetic capacity, and lower HO contents than those of male leaves. Under salinity stress, sodium (Na) accumulation in female leaves was lower than that in the male leaves. The non-invasive micro-test showed that the net Na efflux in the salt-treated female roots was higher than that in male roots. Physiological responses revealed that female cuttings were more tolerant than males, which may be mainly due to females having lower leaf Na accumulation and higher root Na efflux capacity than males. Transcriptional analyses showed that 108 differentially expressed salt-responsive genes were identified in both female and male roots; most of these showed sexual differences in expression patterns under salinity stress. RNA-seq combined with qPCR analysis showed that the salt-induced expression of four Na/H antiporter () genes () in female roots was higher than that in male roots. Transcriptional analyses revealed that the higher Na efflux capacity in female roots than in male roots may be closely related to the differential expression of salt-responsive genes, especially genes.
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http://dx.doi.org/10.3389/fpls.2020.517962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7604296PMC
October 2020

LncRNA MEG3 regulates microglial polarization through KLF4 to affect cerebral ischemia-reperfusion injury.

J Appl Physiol (1985) 2020 12 12;129(6):1460-1467. Epub 2020 Nov 12.

Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

This study aimed to explore whether long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) affects the polarization of microglia in cerebral ischemia-reperfusion (I/R) injury through regulating Krüppel-like factor 4 (KLF4). A middle cerebral artery occlusion/reperfusion-induced (MCAO/R-induced) mouse model was established as an in vivo model. Oxygen and glucose confinement/reoxygenation-induced (OGD/R-induced) microglia (BV2 cells) were used as an in vitro model. RNA pull-down and RNA immunoprecipitation were used to detect the binding between MEG3 and KLF4. The MEG3 expression was signally elevated in the MCAO/R-induced mice or OGD/R-induced BV2 cells. The inhibition of MEG3 reversed the effects of OGD/R injury on the polarization and inflammation of BV2 cells. Moreover, MEG3 bound to KLF4 and inhibited its protein expression. Furthermore, the overexpression of MEG3 promoted M1 polarization and inflammation but inhibited M2 polarization by inhibiting KLF4 in BV2 cells. The transfection of small interfering RNAs against MEG3 inhibited M1 polarization and inflammation and promoted M2 polarization in vitro and in vivo. Inhibition of MEG3 can alleviate cerebral I/R injury via regulating the polarization of microglia through KLF4. To study the role of long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) in cerebral ischemia-reperfusion (I/R) injury, we clarified the mechanism by which lncRNA MEG3 regulates the secretion of inflammatory cytokines in microglia through in vitro and in vivo experiments. We discovered that inhibition of MEG3 could alleviate cerebral I/R injury via inhibiting M1 polarization and promoting M2 polarization through Krüppel-like factor 4 (KLF4), indicating an effective theoretical basis for potential therapeutic targets of cerebral I/R injury.
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http://dx.doi.org/10.1152/japplphysiol.00433.2020DOI Listing
December 2020

Testosterone amendment alters metabolite profiles of the soil microbial community.

Environ Pollut 2021 Mar 26;272:115928. Epub 2020 Oct 26.

State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing, 100085, China. Electronic address:

Steroid hormones are prevalent in the environment and have become emerging pollutants, but little is known about their effects on soil microbial community composition and function. In the present study, three representative soils in China were amended with environmentally relevant concentrations of testosterone and responses of soil bacterial community composition and soil function were assessed using high-throughput sequencing and nontargeted metabolomics. Our results showed that testosterone exposure significantly shifted bacterial community structure and metabolic profiles in soils at Ningbo (NB) and Kunming (KM), which may reflect high bioavailability of the hormone. Abundances of several bacterial taxa associated with nutrient cycling were reduced by testosterone and metabolites related to amino acid metabolism were downregulated. A close connection between bacterial taxa and specific metabolites was observed and confirmed by Procrustes tests and a co-occurrence network. These results provide an insight into the effects of steroid hormones on soil microbial community and highlight that nontargeted metabolomics is an effective tool for investigating the impacts of pollutants.
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http://dx.doi.org/10.1016/j.envpol.2020.115928DOI Listing
March 2021

Morphological and physiological responses of two willow species from different habitats to salt stress.

Sci Rep 2020 10 26;10(1):18228. Epub 2020 Oct 26.

College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.

Plant salt tolerance is a complex mechanism, and different plant species have different strategies for surviving salt stress. In the present study, we analyzed and compared the morphological and physiological responses of two willow species (Salix linearistipularis and Salix matsudana) from different habitats to salt stress. S. linearistipularis exhibited higher seed germination rates and seedling root Na efflux than S. matsudana under salt stress. After salt treatment, S. linearistipularis leaves exhibited less Na accumulation, loss of water and chlorophyll, reduction in photosynthetic capacity, and damage to leaf cell structure than leaves of S. matsudana. Scanning electron microscopy combined with gas chromatography mass spectrometry showed that S. linearistipularis leaves had higher cuticular wax loads than S. matsudana leaves. Overall, our results showed that S. linearistipularis had higher salt tolerance than S. matsudana, which was associated with different morphological and physiological responses to salt stress. Furthermore, our study suggested that S. linearistipularis could be a promising tree species for saline-alkali land greening and improvement.
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http://dx.doi.org/10.1038/s41598-020-75349-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588438PMC
October 2020

D-dimer and its Combination with Blood Lipid on Prognosis of Patients with Acute Ischemic Stroke.

J Stroke Cerebrovasc Dis 2020 Dec 21;29(12):105394. Epub 2020 Oct 21.

Department of Neurology, the first Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address:

Background: Previous studies indicate that the levels of d-dimer and blood lipids at admission affect the prognosis of patients with acute ischemic stroke (AIS), however, whether there is a dose-response effect of d-dimer on prognosis, or a combined effect of d-dimer with blood lipids on prognosis, remains unclear.

Methods: In this prospective cohort study, 1485 AIS patients were recruited. All participants received medical care within 24 h from the onset of stroke, the level of d-dimer and related indices were measured at admission. Then, National Institutes of Health Stroke Scale (NIHSS) scores were obtained at the time of admission and discharge. Afterwards, 3-, 6- and 12- month follow-up was conducted to obtain Modified Rankin Scale (mRS) scores after discharge.

Results: A high level of d-dimer at admission was associated with clinical outcome of AIS, after adjusting other relevant factors, with an OR (95%CI) of 2.934(1.914-4.500), 3.052(1.912-4.872), 3.306(1.873-5.835) and 2.828(1.447-5.527) at discharge, at 3-, 6-, and 12-month follow-up respectively, a dose-response effect was observed during follow-up (p = 0.00001). When d-dimer was combined with total cholesterol (TC), after adjusting other relevant factors, OR (95%CI) was 2.799 (1.708-4.587), 2.473 (1.475-4.147), 2.381 (1.333-4.255), and 2.619 (1.320-5.193), at each follow-up period respectively. When combined with low-density lipoprotein (LDL), OR (95%CI) was 3.105 (1.729-5.577), 3.280 (1.762-6.104), 2.744 (1.344-5.604), and 4.400 (1.883-10.282), respectively.

Conclusions: D-dimer levels at admission may predict the prognosis of AIS patients in a dose-response pattern. Moreover, d-dimer combined with TC or LDL predict prognosis of AIS.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2020.105394DOI Listing
December 2020

Circular RNA CircHIPK3 Elevates CCND2 Expression and Promotes Cell Proliferation and Invasion Through miR-124 in Glioma.

Front Genet 2020 28;11:1013. Epub 2020 Aug 28.

Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

As a malignant tumor of the central nervous system, glioma exhibits high incidence and poor prognosis. Circular RNA HIPK3 (circHIPK3) is a circular RNA (circRNA) related to cancer progression. However, the role of circHIPK3 in gliomas remains unclear. The purpose of this study was to investigate the role of circHIPK3 in gliomas and its mechanism. The qRT-PCR method was used to determine the expression pattern of circHIPK3 in glioma cell lines. CCK-8 assay was used to detect cell proliferation. Cell migration and invasion were evaluated using the Transwell assay. Our results showed that circHIPK3 expression was significantly up-regulated in glioma tissues and cell lines. , the down-regulation of circHIPK3 significantly inhibited the proliferation, migration and invasion of glioma cells. Besides, we demonstrated that circHIPK3 acted as a sponge to absorb miR-124 and promoted CCND2 expression. In summary, our results indicated that circHIPK3 had carcinogenic effects by regulating the expression of CCND2 in glioma by sponging miR-124. These findings provided favorable evidence to reveal the role of circHIPK3 in the development of gliomas.
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http://dx.doi.org/10.3389/fgene.2020.01013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485042PMC
August 2020

The effects of coupled B fields in B encoded TRASE MRI - A simulation study.

Magn Reson Imaging 2020 12 12;74:74-83. Epub 2020 Sep 12.

Space MRI Lab, Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, Canada. Electronic address:

Transmit Array Spatial Encoding (TRASE) is a novel MRI technique that encodes spatial information by introducing phase gradients in the transmit RF (B) magnetic field. Since TRASE relies on the use of multiple RF fields (B fields with different phase gradients) for k-space traversal, a TRASE pulse sequence requires RF pulses that are produced by switching between the transmit coils (B fields). However, interactions among the transmit RF coils can cause un-driven coils to produce unwanted B fields that impair the spatial encoding. Therefore, TRASE is sensitive to B field perturbations arising from inductive coupling among the RF transmit coils and any B field isolation (coil decoupling) technique requires an understanding of the effects of the B field interactions. The purpose of this study was to investigate the effects of B field coupling using Bloch equation based simulations and to determine the acceptable level of B field interactions for 2D TRASE imaging. The simulations show that 2D TRASE MRI (using a 3-coil setup) displays ideal performance for pairwise coupling constant lower than k = 0.01 while having acceptable performance up to k = 0.1. This translates into S measurements of range ~(- 50 dB to -30 dB) required for successful 2D TRASE MRI in this study. This result is of crucial importance for designers of practical TRASE transmit array systems.
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http://dx.doi.org/10.1016/j.mri.2020.09.003DOI Listing
December 2020

Effect of temperatures and alternating anoxic/oxic sequencing batch reactor (SBR) operating modes on extracellular polymeric substances in activated sludge.

Water Sci Technol 2020 Jul;82(1):120-130

School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.

In order to investigate the effect of temperatures and operating modes on extracellular polymeric substances (EPS) contents, three sequencing batch reactors (SBRs) were operated at temperatures of 15, 25, and 35 °C (R, R, and R, respectively), with two SBRs operated under alternating anoxic/oxic conditions (R and R, respectively). Results showed that higher contents of tightly bound EPS (TB-EPS) and total EPS appeared in R, while loosely bound EPS (LB-EPS) dominated in R. In all three kinds of EPS (LB-EPS, TB-EPS and total EPS) assessed, protein was the main component in R and R, while polysaccharides dominated in R. Moreover, compared with R, R was favorable for the production of the three kinds of EPS. Furthermore, three kinds of EPS and their components were augmented during the nitrification process, while they declined during the denitrification process under all conditions except for R.
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http://dx.doi.org/10.2166/wst.2020.336DOI Listing
July 2020

Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins.

J Genet Genomics 2020 06 20;47(6):337-340. Epub 2020 May 20.

MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China; Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences, and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, China. Electronic address:

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http://dx.doi.org/10.1016/j.jgg.2020.05.005DOI Listing
June 2020

Highly sensitive detection of carcinoembryonic antigen using copper-free click chemistry on the surface of azide cofunctionalized graphene oxide.

Anal Chim Acta 2020 Aug 10;1127:156-162. Epub 2020 Jul 10.

Precision Medical Center Laboratory, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325015, China.

In this study, we reported a highly sensitive method for detecting carcinoembryonic antigen (CEA) based on an azide cofunctionalized graphene oxide (GO-N) and carbon dot (CDs) biosensor system. Carbon dots-labeled DNA (CDs-DNA) combined with GO-N using copper-free click chemistry (CFCC), which quenched the fluorescence of the CDs via fluorescence resonance energy transfer (FRET). Upon the addition of CEA, fluorescence was recovered due to the combination of CEA and aptamer. Under optimal conditions, the relative fluorescence intensity was linear with CEA concentration in the range of 0.01-1 ng/mL (R = 0.9788), and the limit of detection (LOD) was 7.32 pg/mL (S/N = 3). This biosensor had a high sensitivity and good selectivity for CEA detection in serum samples, indicating that the novel sensor platform holds a great potential for CEA and other biomarkers in practical applications.
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http://dx.doi.org/10.1016/j.aca.2020.06.053DOI Listing
August 2020

Comparison of methods for the detection of outliers and associated biomarkers in mislabeled omics data.

BMC Bioinformatics 2020 Aug 14;21(1):357. Epub 2020 Aug 14.

Department of Health Statistics, School of Public Health, Shanxi Medical University, Taiyuan City, 030001, Shanxi, China.

Background: Previous studies have reported that labeling errors are not uncommon in omics data. Potential outliers may severely undermine the correct classification of patients and the identification of reliable biomarkers for a particular disease. Three methods have been proposed to address the problem: sparse label-noise-robust logistic regression (Rlogreg), robust elastic net based on the least trimmed square (enetLTS), and Ensemble. Ensemble is an ensembled classification based on distinct feature selection and modeling strategies. The accuracy of biomarker selection and outlier detection of these methods needs to be evaluated and compared so that the appropriate method can be chosen.

Results: The accuracy of variable selection, outlier identification, and prediction of three methods (Ensemble, enetLTS, Rlogreg) were compared for simulated and an RNA-seq dataset. On simulated datasets, Ensemble had the highest variable selection accuracy, as measured by a comprehensive index, and lowest false discovery rate among the three methods. When the sample size was large and the proportion of outliers was ≤5%, the positive selection rate of Ensemble was similar to that of enetLTS. However, when the proportion of outliers was 10% or 15%, Ensemble missed some variables that affected the response variables. Overall, enetLTS had the best outlier detection accuracy with false positive rates < 0.05 and high sensitivity, and enetLTS still performed well when the proportion of outliers was relatively large. With 1% or 2% outliers, Ensemble showed high outlier detection accuracy, but with higher proportions of outliers Ensemble missed many mislabeled samples. Rlogreg and Ensemble were less accurate in identifying outliers than enetLTS. The prediction accuracy of enetLTS was better than that of Rlogreg. Running Ensemble on a subset of data after removing the outliers identified by enetLTS improved the variable selection accuracy of Ensemble.

Conclusions: When the proportion of outliers is ≤5%, Ensemble can be used for variable selection. When the proportion of outliers is > 5%, Ensemble can be used for variable selection on a subset after removing outliers identified by enetLTS. For outlier identification, enetLTS is the recommended method. In practice, the proportion of outliers can be estimated according to the inaccuracy of the diagnostic methods used.
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http://dx.doi.org/10.1186/s12859-020-03653-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646480PMC
August 2020

Effects of BDNF Signaling on Anxiety-Related Behavior and Spatial Memory of Adolescent Rats in Different Length of Maternal Separation.

Front Psychiatry 2020 23;11:709. Epub 2020 Jul 23.

Department of Psychology, Weifang Medical University, Weifang, China.

As an adverse form of early-life stress (ELS), maternal separation (MS) can interfere with the development of cognition and behaviors of adolescent rodents. Brain-derived neurotrophic factor (BDNF) is involved in the regulation of brain development and function, but the molecular mechanisms by which BDNF regulates brain function and behavior in MS with different stressor strengths remain unclear. This descriptive study characterized the levels of BDNF in the prefrontal cortex (PFC) and plasma corticosterone (CORT) from the offspring of rats exposed to early handling (EH, 15-min separation per day) and prolonged MS (PMS, 180-min separation per day), during postnatal days (PND) 1‑21. The behavioral and biochemical analyses were performed during adolescence (PND 42‑56). PMS resulted in reduced weight and decreased locomotor activity in the open field test and Y-maze task compared to control (CON) group, with EH showing an intermediate phenotype. BDNF protein levels in the PFC were lower in PMS compared to EH and further reduced in CON male rats. Plasma CORT levels were higher in PMS compared to CON with EH again showing intermediate levels. Neither PMS or EH affected spatial learning in the Y-maze task. These findings indicate that longer periods of maternal separation are necessary to increase anxiety-like behavior, elevate CORT levels, and further suppress BDNF levels in the PFC, providing a possible mechanism to explain why more severe forms of ELS lead to more significant psychiatric and medical consequences later in life.
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http://dx.doi.org/10.3389/fpsyt.2020.00709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391957PMC
July 2020

Combined prognostic significance of D-dimer level and platelet count in acute ischemic stroke.

Thromb Res 2020 10 17;194:142-149. Epub 2020 May 17.

Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China. Electronic address:

Background: D-dimer level and platelet count (PC) have been reported separately as significant independent predictors of Acute Ischemic Stroke (AIS). Here, we aimed to investigate the combined prognostic value of abnormal D-dimer level and PC as defined for specific in-hospital and long-term outcomes in AIS patients.

Methods: A total of 1468 patients admitted for ischemic stroke within 24 h of symptom onset from April 1, 2016 to November 31, 2019 at the Department of Neurology, the First Affiliated Hospital of Harbin Medical University were included in the final analysis. Eligible subjects were divided into four groups in terms of their levels of D-dimer and PC: DD-PC- (normal D-dimer level and normal PC), DD-PC+ (normal D-dimer level and abnormal PC), DD+PC- (higher D-dimer level and normal PC), and DD+PC+ (higher D-dimer level and abnormal PC). Logistic regression model and multinomial logit model were used to estimate the combined effect of D-dimer level and PC on in-hospital outcomes including discharge outcome and early neurological changes, and poor outcomes at 3, 6 and 12 months.

Results: DD+PC+ was found to be associated with the risk of in-hospital mortality (adjusted odds ratio [OR], 6.904; 95% confidence interval [CI], 2.781-17.144) and 3-month mortality (adjusted OR, 5.455; 95% CI, 2.019-14.743) compared with DD-PC-. Combination of the two indicators significantly improved the independent predictive value for functional outcomes, including early neurological deterioration (END) (OR, 3.622; 95% CI, 1.732-7.573) with threshold of at least 4-point increase on NIHSS, discharge outcome (OR, 2.713; 95% CI, 1.421-5.177); mRS of 0-1 point (OR, 0.409; 95% CI, 0.211-0.792), mRS of 0-2 points (OR, 0.234; 95% CI, 0.118, 0.461), and higher mRS-shift (OR, 2.379; 95% CI, 1.237-4.576) at 3 months; unfavorable outcome at 3 months (OR, 4.280; 95% CI, 2.169-8.446), 6 months (OR, 3.297; 95% CI, 1.452-7.488) and 12 months (OR, 4.157; 95% CI, 1.598-10.816). While comparatively weaker statistical significance was shown in DD+PC- and no correlation was found between adverse outcomes and DD-PC+. Similarly, patients with abnormal D-dimer level and PC were less likely to reach the status of stable or improving.

Conclusions: Combination of D-dimer level and PC may have more significant prognostic value on END, in-hospital mortality, discharge outcome, and long-term outcomes than either index of D-dimer level or PC alone in AIS patients.
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http://dx.doi.org/10.1016/j.thromres.2020.05.021DOI Listing
October 2020

Screening and verification of long noncoding RNA promoter methylation sites in hepatocellular carcinoma.

Cancer Cell Int 2020 15;20:311. Epub 2020 Jul 15.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province People's Republic of China.

Background: Long noncoding ribonucleic acid (lncRNA) promoter methylation is closely related to the occurrence and development of hepatocellular carcinoma (HCC). Thus, we aim to screen and verify the lncRNA promoter methylation sites associated with overall survival (OS), vascular invasion, pathological grade, and clinical stage in HCC.

Methods: Methylation-related data including clinical characteristic, transcriptome, methylation, and messenger RNA (mRNA) expression were taken from the Cancer Genome Atlas (TCGA) database. The OS, vascular invasion, pathological grade, and clinical stage-related lncRNA promoter methylation models were developed by the least absolute shrinkage and selection operator (LASSO) algorithm based on the lncRNA promoter methylation sites screened via R software. The Kaplan-Meier analysis, the area under the receiver operating characteristic (ROC) curve (AUC), the calibration curve (C-index) were performed to evaluate the performance of these models. Finally, the methylation-specific polymerase chain reaction (MS-PCR) was performed to verify the accuracy of these models based on 146 HCC tissues from our hospital.

Results: A total of 10 methylation sites were included in the OS-related lncRNA promoter methylation model that could effectively divide HCC patients into high-risk and low-risk groups (P < 0.0001) via survival analysis. COX univariable and multivariable regression analysis found that the OS-related model (P < 0.001, 95% CI 1.378-2.942) and T stage (P < 0.001, 95% CI 1.490-3.418) were independent risk factors affecting OS in HCC patients. The vascular invasion-related model contained 8 methylation sites with its AUC value of 0.657; the pathological grade-related model contained 22 methylation sites with its AUC value of 0.797; the clinical stage-related model contained 13 methylation sites with its AUC of 0.724. Target genes corresponded to vascular invasion-related lncRNA promoter methylation sites were involved in many kinds of biological processes in HCC such as PI3K-Akt signaling pathway. The accuracy of the vascular invasion-related model was consistent with our bioinformatics conclusion after being verified via MS-PCR.

Conclusion: The lncRNA promoter methylation sites are closely correlated with the process of HCC and can be utilized to improve the therapy and prognosis of HCC.
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http://dx.doi.org/10.1186/s12935-020-01407-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362420PMC
July 2020

Expert consensus on protocol of rehabilitation for COVID-19 patients using framework and approaches of WHO International Family Classifications.

Aging Med (Milton) 2020 Jun 6;3(2):82-94. Epub 2020 Jul 6.

Guangdong People's Hospital Guangdong Academy of Medical Sciences Guangzhou China.

Coronavirus disease 2019 (COVID-19) has widely spread all over the world and the numbers of patients and deaths are increasing. According to the epidemiology, virology, and clinical practice, there are varying degrees of changes in patients, involving the human body structure and function and the activity and participation. Based on the World Health Organization (WHO) International Classification of Functioning, Disability and Health (ICF) and its biopsychosocial model of functioning, we use the WHO Family of International Classifications (WHO-FICs) framework to form an expert consensus on the COVID-19 rehabilitation program, focusing on the diagnosis and evaluation of disease and functioning, and service delivery of rehabilitation, and to establish a standard rehabilitation framework, terminology system, and evaluation and intervention systems based the WHO-FICs.
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http://dx.doi.org/10.1002/agm2.12120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338700PMC
June 2020

Toxic effects of heavy metal Cd and Zn on chlorophyll, carotenoid metabolism and photosynthetic function in tobacco leaves revealed by physiological and proteomics analysis.

Ecotoxicol Environ Saf 2020 Oct 3;202:110856. Epub 2020 Jul 3.

Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang, China. Electronic address:

To explore the mechanisms underlying the action of the heavy metals Cd and Zn on the photosynthetic function of plant leaves, the effects of 100 μmol L Cd and 200 μmol L Zn stress (the exposure concentrations of Cd and Zn in the culture medium were 2.24 mg kg and 5.36 mg kg) on the chlorophyll and carotenoid contents as well as the photosynthetic function of tobacco leaves (Long Jiang 911) were studied. The key proteins in these physiological processes were quantitatively analyzed using a TMT-based proteomics approach. Cd stress was found to inhibit the expression of key enzymes during chlorophyll synthesis in leaves, resulting in a decrease of the Chl content. However, Zn stress did not significantly influence the chlorophyll content. Leaves adapted to Zn stress by upregulating CAO expression and increase the Chl b content. Although the Car content in leaves did not significantly change under either Cd or Zn stress, the expressions of ZE and VDE during Car metabolism decreased significantly under Cd stress. This was accompanied by damages to the xanthophyll cycle and the NPQ-dependent energy dissipation mechanism. In contrast, under Zn stress, leaves adapted to Zn stress by increasing the expression of VDE, thus improving NPQ. Under Cd stress, the expressions of three sets of proteins were significantly down-regulated, including PSII donor-side proteins (PPD3, PPD6, OEE1, OEE2-1, OEE2-2, OEE2-3, and OEE3-2), receptor-side proteins (D1, D2, CP43, CP47, Cyt b559α, Cyt b559β, PsbL, PsbQ, PsbR, Psb27-H1, and Psb28), and core proteins of the PSI reaction center (psaA, psaB, psaC, psaD, psaE-A, PsaE-B, psaF, psaG, psaH-1, psaK, psaL, psaN, and psaOL). In comparison, only eight of the above proteins (PPD6, OEE3-2, PsbL, PsbQ, Psb27-H1, psaL, and psaOL) were significantly down-regulated by Zn stress. Under Cd stress, both the donor side and the receptor side of PSII were damaged, and PSII and PSI experienced severe photoinhibition. However, Zn stress did not decrease either PSII or PSI activities in tobacco leaves. In addition, the expression of electron transport-related proteins (cytb6/f complex, PC, Fd, and FNR), ATPase subunits, Rubisco subunits, and RCA decreased significantly in leaves under Cd stress. However, no significant changes were observed in any of these proteins under Zn stress. Although Cd stress was found to up-regulate the expressions of PGRL1A and PGRL1B and induce an increase of PGR5/PGRL1-CEF in tobacco leaves, NDH-CEF was significantly inhibited. Under Zn stress, the expressions of ndhH and PGRL1A in leaves were significantly up-regulated, but there were no significant changes in either NDH-CEF or PGR5/PGRL-CEF. Under Cd stress, the expressions of proteins related to Fd-dependent nitrogen metabolism and reactive oxygen species (ROS) scavenging processes (e.g., FTR, Fd-NiR, and Fd-GOGAT) were significantly down-regulated in leaves. However, no significant changes of any of the above proteins were identified under Zn stress. In summary, Cd stress could inhibit the synthesis of chlorophyll in tobacco leaves, significantly down-regulate the expressions of photosynthesis-related proteins or subunits, and suppress both the xanthophyll cycle and NDH-CEF process. The expressions of proteins related to the Fd-dependent nitrogen metabolism and ROS scavenging were also significantly down-regulated, which blocked the photosynthetic electron transport, thus resulting in severe photoinhibition of both PSII and PSI. However, Zn stress had little effect on the photosynthetic function of tobacco leaves.
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http://dx.doi.org/10.1016/j.ecoenv.2020.110856DOI Listing
October 2020

Self-reported snoring is associated with nonalcoholic fatty liver disease.

Sci Rep 2020 06 9;10(1):9267. Epub 2020 Jun 9.

Department of Epidemiology and Health Statistics, School of Public Health, Shanxi Medical University, Taiyuan, 030001, China.

Although nonalcoholic fatty liver disease (NAFLD) is associated with obstructive sleep apnea syndrome (OSAS), studies on the direct relationship between NAFLD and snoring, an early symptom of OSAS, are limited. We evaluated whether snorers had higher risk of developing NAFLD. The study was performed using data of the Tongmei study (cross-sectional survey, 2,153 adults) and Kailuan study (ongoing prospective cohort, 19,587 adults). In both studies, NAFLD was diagnosed using ultrasound; snoring frequency was determined at baseline and classified as none, occasional (1 or 2 times/week), or habitual (≥3 times/week). Odds ratios (ORs) and hazard ratios (HRs) with 95% confidence intervals were estimated using logistic and Cox models, respectively. During 10 years' follow-up in Kailuan, 4,576 individuals with new-onset NAFLD were identified at least twice. After adjusting confounders including physical activity, perceived salt intake, body mass index (BMI), and metabolic syndrome (MetS), multivariate-adjusted ORs and HRs for NAFLD comparing habitual snorers to non-snorers were 1.72 (1.25-2.37) and 1.29 (1.16-1.43), respectively. These associations were greater among lean participants (BMI < 24) and similar across other subgroups (sex, age, MetS, hypertension). Snoring was independently and positively associated with higher prevalence and incidence of NAFLD, indicating that habitual snoring is a useful predictor of NAFLD, particularly in lean individuals.
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http://dx.doi.org/10.1038/s41598-020-66208-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283303PMC
June 2020

Overexpression of Trx CDSP32 gene promotes chlorophyll synthesis and photosynthetic electron transfer and alleviates cadmium-induced photoinhibition of PSII and PSI in tobacco leaves.

J Hazard Mater 2020 11 16;398:122899. Epub 2020 May 16.

College of Life Science, Northeast Forest University, Harbin, Heilongjiang, China.

Cadmium stress causes a decrease in chlorophyll content and inhibits photosynthesis in tobacco leaves. The role of thioredoxin-like protein CDSP32 expressed in plant chloroplasts is to alleviates the reduced enzymes expression involved in chlorophyll synthesis of tobacco leaves due to Cd exposure, effectively preventing chlorophyll degradation and promoting increased tobacco biomass. Overexpression of Trx CDSP32 can protect the oxygen-evolving complex on the PSII donor side and promote electron transfer on the PSII acceptor side of tobacco leaves under Cd stress. Trx CDSP32 not only significantly increase the PSI activity of tobacco leaves, but also alleviate cadmium-induced PSI photoinhibition. Although Trx CDSP32 has no significant effect on the expression of PC and FNR proteins in tobacco leaves under Cd stress, it can alleviate the decreased expression of protein subunits involved in photosynthetic electron transfer such as Cyt b6/f complex subunits, Fd, and ATP synthase subunits. Trx CDSP32 can promote the synthesis of chlorophyll, stabilize the electron transfer chain, and promote ATP synthase activity to alleviate cadmium-induced photoinhibition of PSII and PSI in tobacco leaves.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122899DOI Listing
November 2020