Publications by authors named "Hongwei Ma"

174 Publications

Preservation of endoplasmic reticulum (ER) Ca stores by deletion of inositol-1,4,5-trisphosphate receptor type 1 promotes ER retrotranslocation, proteostasis, and protein outer segment localization in cyclic nucleotide-gated channel-deficient cone photoreceptors.

FASEB J 2021 Jun;35(6):e21579

Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

Endoplasmic reticulum (ER) Ca homeostasis relies on an appropriate balance between efflux- and influx-channel activity responding to dynamic changes of intracellular Ca levels. Dysregulation of this complex signaling network has been shown to contribute to neuronal and photoreceptor death in neuro- and retinal degenerative diseases, respectively. In mice with cone cyclic nucleotide-gated (CNG) channel deficiency, a model of achromatopsia/cone dystrophy, cones display early-onset ER stress-associated apoptosis and protein mislocalization. Cones in these mice also show reduced cytosolic Ca level and subsequent elevation in the ER Ca -efflux-channel activity, specifically the inositol-1,4,5-trisphosphate receptor type 1 (IP R1), and deletion of IP R1 results in preservation of cones. This work investigated how preservation of ER Ca stores leads to cone protection. We examined the effects of cone specific deletion of IP R1 on ER stress responses/cone death, protein localization, and ER proteostasis/ER-associated degradation. We demonstrated that deletion of IP R1 improves trafficking of cone-specific proteins M-/S-opsin and phosphodiesterase 6C to cone outer segments and reduces localization to cone inner segments. Consistent with the improved protein localization, deletion of IP R1 results in increased ER retrotranslocation protein expression, reduced proteasome subunit expression, reduced ER stress/cone death, and reduced retinal remodeling. We also observed the enhanced ER retrotranslocation in mice that have been treated with a chemical chaperone, supporting the connection between improved ER retrotranslocation/proteostasis and alleviation of ER stress. Findings from this work demonstrate the importance of ER Ca stores in ER proteostasis and protein trafficking/localization in photoreceptors, strengthen the link between dysregulation of ER Ca homeostasis and ER stress/cone degeneration, and support an involvement of improved ER proteostasis in ER Ca preservation-induced cone protection; thereby identifying IP R1 as a critical mediator of ER stress and protein mislocalization and as a potential target to preserve cones in CNG channel deficiency.
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http://dx.doi.org/10.1096/fj.202002711RDOI Listing
June 2021

A four-hypoxia-genes-based prognostic signature for oral squamous cell carcinoma.

BMC Oral Health 2021 May 3;21(1):232. Epub 2021 May 3.

Department of Oral and Maxillofacial Surgery, Tianjin Stomatology Hospital, School of Medicine, NanKai University, Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, No. 75, Dagu North Road, Heping District, Tianjin, 300041, China.

Background: Oral squamous cell carcinoma (OSCC) is one of the most common maligancies of the head and neck. The prognosis was is significantly different among OSCC patients. This study aims to identify new biomarkers to establish a prognostic model to predict the survival of OSCC patients.

Methods: The mRNA expression and corresponding clinical information of OSCC patients were downloaded from The Cancer Genome Atlas and Gene Expression Omnibus. Additionally, a total of 26 hypoxia-related genes were also obtained from a previous study. Univariate Cox regression analysis and LASSO Cox regression analysis were performed to screen the optimal hypoxia-related genes which were associated with the prognosis of OSCC. to establish the predictive model (Risk Score) was established for estimating the patient's overall survival (OS). Multivariate Cox regression analysis was used to determine whether the Risk Score was an independent prognostic factor. Based on all the independent prognostic factors, nomogram was established to predict the OS probability of OSCC patients. The relative proportion of 22 immune cell types in each patient was evaluated by CIBERSORT software.

Results: We determined that a total of four hypoxia-related genes including ALDOA, P4HA1, PGK1 and VEGFA were significantly associated with the prognosis of OSCC patients. The nomogram established based on all the independent factors could reliably predict the long-term OS of OSCC patients. In addition, our resluts indicated that the inferior prognosis of OSCC patients with high Risk Score might be related to the immunosuppressive microenvironments.

Conclusion: This study shows that high expression of hypoxia-related genes including ALDOA, P4HA1, PGK1 and VEGFA is associated with poor prognosis in OSCC patients, and they can be used as potential markers for predicting prognosis in OSCC patients.
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http://dx.doi.org/10.1186/s12903-021-01587-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094530PMC
May 2021

Application of New Radiosensitizer Based on Nano-Biotechnology in the Treatment of Glioma.

Front Oncol 2021 22;11:633827. Epub 2021 Mar 22.

Institute of Nervous System Diseases, Xuzhou Medical University, Xuzhou, China.

Glioma is the most common intracranial malignant tumor, and its specific pathogenesis has been unclear, which has always been an unresolved clinical problem due to the limited therapeutic window of glioma. As we all know, surgical resection, chemotherapy, and radiotherapy are the main treatment methods for glioma. With the development of clinical trials and traditional treatment techniques, radiotherapy for glioma has increasingly exposed defects in the treatment effect. In order to improve the bottleneck of radiotherapy for glioma, people have done a lot of work; among this, nano-radiosensitizers have offered a novel and potential treatment method. Compared with conventional radiotherapy, nanotechnology can overcome the blood-brain barrier and improve the sensitivity of glioma to radiotherapy. This paper focuses on the research progress of nano-radiosensitizers in radiotherapy for glioma.
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http://dx.doi.org/10.3389/fonc.2021.633827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044949PMC
March 2021

Numerical study of probe parameters on performance of a transonic axial compressor.

PLoS One 2021 25;16(1):e0245711. Epub 2021 Jan 25.

School of Energy and Power Engineering, Beihang University, Beijing, China.

The paper shows the effect of the probe on the performance of a transonic axial speed compressor. The unobstructed flow case with the experimental data was validated and used as a guide for all subsequent study cases. The aerodynamic performance for different probe parameters were calculated numerically using ANSYS-CFX. This covered the results on compressor output from changing probe axial positions, the radial immersion depths, the size of the probe, and the total number of probes. The findings were evaluated in relation to the total pressure ratio, performance, margin of deflation and stability. The velocity part distributions further showed that the probe block and raises the flow Mach value, which is the explanation why the compressor rotor's total pressure ratio is lost. In fact, the parameters of the sample will significantly influence the calculation outcomes and affect the standard margin. The range of stability was also affected, which changes the performance trend from the choke to the stall. Consequently, the collection of correct probe parameters with fewer impact on compressor output is addressed.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245711PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833163PMC
January 2021

[Effectiveness of convalescent plasma for treatment of coronavirus disease 2019 patients].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Nov;32(11):1293-1298

Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.

Objective: To evaluate the effectiveness and safety of convalescent plasma therapy in patients with severe and critical coronavirus disease 2019 (COVID-19).

Methods: Plasma of 200-400 mL was collected from convalescent patients 2 weeks after being discharged from the hospital. After viral nucleic acid testing and antibody testing, the plasma was infused into 16 severe or critical COVID-19 patients. Time for viral nucleic acid amplification (NAA) test turning negative, total volume of plasma transfusion, average antibody concentration, and total antibody amount were recorded. White blood cell (WBC) counts, lymphocyte (LYM) counts, neutrophil (NEU) counts, alanine aminotransferase (ALT), aspartate aminotransferase (AST), C-reactive protein (CRP), procalcitonin (PCT), lactate dehydrogenase (LDH), creatine kinase (CK), creatine kinase isoenzyme (CK-MB), hypersensitive cardiac troponin T (hs-cTnT), and lactic acid (Lac) levels were measured and the rate of change was calculated at the baseline (d0) before plasma transfusion, and day 1 (d1), day 3 (d3) after transfusion.

Results: (1) Patient characteristics: among the 16 patients, 5 cases (31.25%) were severe COVID-19, and 11 cases (68.75%) were critical COVID-19; 62.50% (10/16) of the patients had primary disease; the percentage of invasive ventilation and use of extracorporeal membrane pulmonary oxygenation (ECMO) in critical patients were 90.91% (10/11) and 45.46% (5/11) respectively. (2) Antibody concentration of convalescent plasma and time for NAA test turning negative: the convalescent plasma antibody concentration in this study was ranged from 10.93 kAU/L to 114.7 kAU/L, with an average value of (56.44±39.40) kAU/L. NAA test was continuously positive before plasma transfusion in 10 patients, and the time for NAA test turning negative could be counted. Eight patients turned negative from day 2 to day 8 after transfusion. Severe patients showed a shorter time for NAA test turning negative than critical patients after transfusion [2 (2-3) vs. 5 (3-8), P = 0.036]. Two critical patients transfused plasma with lower antibody concentration remained a positive result of NAA test, and died on the 3rd and 6th day respectively. (3) Laboratory results: the change rates of WBC (0.81±0.28 vs. 1.00) and NEU (0.75±0.33 vs. 1.00) were significantly decreased at d1 after convalescent plasma treatment (both P < 0.05), and the CRP level decreased to about 63% of that before transfusion (P = 0.017). No adverse events were observed during convalescent plasma transfusion.

Conclusions: Viral NAA test of most patients with COVID-19 who received convalescent plasma transfusion turned negative from day 2 to day 8 after transfusion, and the turning time of severe patients was shorter than that of critical patients. Convalescent plasma therapy can reduce the patients' CRP level, and no adverse events were found during the treatment. The antibody concentration in the convalescent plasma may be one of the factors that affect the time for the nucleic acid turning negative after transfusion. Detection and screening convalescent plasma of high-titer antibody and early application to severe and critical patients are expected to improve the efficacy of convalescent plasma.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200810-00568DOI Listing
November 2020

Identification of a universal antigen epitope of influenza A virus using peptide microarray.

BMC Vet Res 2021 Jan 7;17(1):22. Epub 2021 Jan 7.

College of Veterinary Medicine, Yangzhou University, 48 East Wenhui Road, Yangzhou, Jiangsu, 225009, People's Republic of China.

Background: Hemagglutinin is a major surface protein in influenza A virus (IAV), and HA2 is relative conserved among different IAVs. It will be meaningful to identify broad-spectrum epitopes based on the HA2 protein.

Results: Overlapping peptides of the HA2 protein of the H5N1 IAV A/Mallard/Huadong/S/2005 were synthesized and loaded on modified silica gel film to form a microarray, and antisera against different subtypes of IAVs were used to screen universal epitopes. The selected epitope was further confirmed by western blotting using anti-peptide immune serum and viruses rescued with amino acid substitution. The results showed that 485-FYHKCDNECME-495 of the H5 14th peptide in HA2 had broad-spectrum binding activity with antisera against H1, H3, H4, H5, H6, H7, H8, H9, and H10 subtype IAV. Substitution of amino acids (K or D) in rescued viruses resulted in decreased serum binding, indicating that they were critical residues for serum binding activity. In Immune Epitope Database, some epitopes containing 14-4 peptide were confirmed as MHC-II-restricted CD4 T cell epitope and had effects on releasing IL-2 or IFN.

Conclusion: The identified epitope should be a novel universal target for detection and vaccine design and its ability to generate immune protection needs further exploration.
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http://dx.doi.org/10.1186/s12917-020-02725-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792037PMC
January 2021

The cGMP-Dependent Protein Kinase 2 Contributes to Cone Photoreceptor Degeneration in the -Deficient Mouse Model of Achromatopsia.

Int J Mol Sci 2020 Dec 23;22(1). Epub 2020 Dec 23.

Department of Pharmacy-Center for Drug Research, Ludwig-Maximilians-University, 81377 Munich, Germany.

Mutations in the gene, which encodes the A subunit of the cyclic guanosine monophosphate (cGMP)-gated cation channel in cone photoreceptor outer segments, cause total colour blindness, also referred to as achromatopsia. Cones lacking this channel protein are non-functional, accumulate high levels of the second messenger cGMP and degenerate over time after induction of ER stress. The cell death mechanisms that lead to loss of affected cones are only partially understood. Here, we explored the disease mechanisms in the knockout (KO) mouse model of achromatopsia. We found that another important effector of cGMP, the cGMP-dependent protein kinase 2 (Prkg2) is crucially involved in cGMP cytotoxicity of cones in KO mice. Virus-mediated knockdown or genetic ablation of in KO mice counteracted degeneration and preserved the number of cones. Analysis of markers of endoplasmic reticulum stress and unfolded protein response confirmed that induction of these processes in KO cones also depends on Prkg2. In conclusion, we identified Prkg2 as a novel key mediator of cone photoreceptor degeneration in achromatopsia. Our data suggest that this cGMP mediator could be a novel pharmacological target for future neuroprotective therapies.
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http://dx.doi.org/10.3390/ijms22010052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793084PMC
December 2020

Corrigendum: Remdesivir (GS-5734) Impedes Enterovirus Replication Through Viral RNA Synthesis Inhibition.

Front Microbiol 2020 23;11:621197. Epub 2020 Nov 23.

Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi'an, China.

[This corrects the article DOI: 10.3389/fmicb.2020.01105.].
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http://dx.doi.org/10.3389/fmicb.2020.621197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732440PMC
November 2020

Experimental Investigation of the Effect of Purge Flow and Main Flow Interaction in a Low-Speed Turbine Cascade Passage.

Entropy (Basel) 2020 Jun 4;22(6). Epub 2020 Jun 4.

School of Energy and Power Engineering, Beihang University, Beijing 100191, China.

In order to protect the vulnerable turbine components from extreme high temperature, coolant flow is introduced from the compressor to the disk cavity, inevitably interacting with the main flow. This paper describes an experimental investigation of the interaction between the main flow and the purge flow in a low-speed turbine cascade with three purge flow rates, Cm = 0, Cm = 1%, and Cm = 2%. In order to study the effect of the interaction between the main flow and the purge flow on the secondary flows, a Rortex method developed by Liu Chaoquan is introduced to identify the vortex in the flow field. In the meantime, a method to calculate the mean entropy production rate based on the particle image velocimetry (PIV) result is adopted to investigate the flow loss. The PIV result indicates that the purge flow has a prominent impact on the flow field of the cascade passage, changing the velocity distribution that induces a local blockage area. The results of vortex identification show that the purge flow promotes the generation of the passage vortex near the suction side. In addition, the purge flow makes the passage vortex migrate to the tip wall direction, enlarging the region affected by the secondary flow. The mean entropy production (MEP) result shows that the flow loss is mainly caused by the passage vortex. The coincidence of the high-MEP region and the location of the passage vortex indicates that the purge flow increases the secondary flow loss by affecting the formation and the migration of the passage vortex.
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http://dx.doi.org/10.3390/e22060623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818119PMC
June 2020

Abnormal neurite orientation dispersion and density imaging of white matter in children with primary nocturnal enuresis.

Neuroimage Clin 2020 20;28:102389. Epub 2020 Aug 20.

Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China.

Several lines of evidence indicate that multiple abnormalities of gray matter are related to the pathogenesis of primary nocturnal enuresis (PNE); however, few studies have been conducted with respect to abnormalities in white matter (WM) of children with PNE. The present work investigated the microstructure of WM in children with PNE using a neurite orientation dispersion and density imaging (NODDI) method. NODDI data were obtained from 29 children with PNE (age = 9.8 ± 1.2 years, 59% males) and 34 healthy controls (age = 10.3 ± 1.6 years, 56% males) in this study. Multi-b-value diffusion-weighted imaging data were acquired with a 3 T MR system, and the orientation dispersion index (ODI) and neurite density index (NDI) maps were calculated. Tract-Based Spatial Statistics analyses of WM tracts were performed with ODI and NDI maps in children with PNE and controls. Children with PNE had lower ODIs in WM fiber tracts of the bilateral superior longitudinal fasciculus (SLF) and higher ODIs in the bilateral internal capsule (IC) and right anterior thalamic radiation (ATR) than controls. PNE children also had lower NDIs in the bilateral IC and the cingulum and higher NDIs in the bilateral SLF. These changes in NODDI indices, which indicated abnormal neural maturation of the WM microstructures, may be related to abnormal sleep and enuresis in children with PNE.
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http://dx.doi.org/10.1016/j.nicl.2020.102389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490590PMC
August 2020

Remdesivir (GS-5734) Impedes Enterovirus Replication Through Viral RNA Synthesis Inhibition.

Front Microbiol 2020 12;11:1105. Epub 2020 Jun 12.

Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi'an, China.

Human enteroviruses are responsible for diverse diseases, from mild respiratory symptoms to fatal neurological complications. Currently, no registered antivirals have been approved for clinical therapy. Thus, a therapeutic agent for the enterovirus-related disease is urgently needed. Remdesivir (GS-5734) is a novel monophosphoramidate adenosine analog prodrug that exhibits potent antiviral activity against diverse RNA virus families, including positive-sense and and negative-sense , , and . Currently, remdesivir is under phase 3 clinical development for disease COVID-19 treatment. Here, we found that remdesivir impeded both EV71 viral RNA (vRNA) and complementary (cRNA) synthesis, indicating that EV71 replication is inhibited by the triphosphate (TP) form of remdesivir. Moreover, remdesivir showed potent antiviral activity against diverse enteroviruses. These data extend the remdesivir antiviral activity to enteroviruses and indicate that remdesivir is a promising antiviral treatment for EV71 and other enterovirus infections.
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http://dx.doi.org/10.3389/fmicb.2020.01105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304253PMC
June 2020

N-methyladenosine modifications enhance enterovirus 71 ORF translation through METTL3 cytoplasmic distribution.

Biochem Biophys Res Commun 2020 06 11;527(1):297-304. Epub 2020 May 11.

Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi'an, 710032, China. Electronic address:

During replication, numerous viral RNAs are modified by N-methyladenosine (mA), the most abundant internal RNA modification. mA is believed to regulate elements of RNA metabolism, such as splicing, stability, translation, secondary structure formation, and viral replication. In this study, we assessed the occurrence of mA modification of the EV71 genome in human cells and revealed a preferred, conserved modification site across diverse viral strains. A single mA modification at the 5' UTR-VP4 junction was shown to perform a protranslational function. Depletion of the METTL3 methyltransferase or treatment with 3-deazaadenosine significantly reduced EV71 replication. Specifically, METTL3 colocalized with the viral dsRNA replication intermediate in the cytoplasm during EV71 infection. As a nuclear resident protein, METTL3 relies on the binding of the nuclear import protein karyopherin to its nuclear localization signal (NLS) for nuclear translocation. We observed that EV71 2A and METTL3 share nuclear import proteins. The results of this study revealed an inner mechanism by which EV71 2A regulates the subcellular location of METTL3 to amplify its own gene expression, providing an increased understanding of RNA epitranscriptomics during the EV71 replication cycle.
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http://dx.doi.org/10.1016/j.bbrc.2020.04.088DOI Listing
June 2020

Cooperative and Independent Effect of Modular Functionalization on Mesomorphic Performances and Microphase Separation of Well-Designed Liquid Crystalline Diblock Copolymers.

Chemistry 2020 Sep 28;26(49):11199-11208. Epub 2020 Jul 28.

State Key Laboratory of Fine Chemicals, Department of Polymer Science and Engineering, Liaoning Key Laboratory of Polymer Science and Engineering, School of Chemical Engineering, Dalian University of Technology, Dalian, Liaoning, 116024, China.

Liquid crystalline block copolymers (LCBCPs) are promising for developing functional materials owing to an assembly of better functionalities. Taking advantage of differences in reactivity between alkynyl and vinyl over temperature during hydrosilylation, a series of LCBCPs with modular functionalization of the block copolymers (BCPs) are reported by independently and site-selectively attaching azobenzene moieties containing alkynyl (LC ) and Si-H (LC ) terminals into well-designed poly(styrene)-block-polybutadienes (PS-b-PBs) and poly(4-vinylphenyldimethylsilane)-block-polybutadienes (PVPDMS-b-PBs) produced from living anionic polymerization (LAP). By the principle of modular functionalization, it is demonstrated that mono-functionalized (PVPDMS-g-LC )-b-PB and PS-b-(PB-g-LC ) not only maintain independence but also have cooperative contributions to bi-functionalized (PVPDMS-g-LC )-b-(PB-g-LC ) in terms of mesomorphic performances and microphase separation, which is evident from differential scanning calorimetry (DSC) and polarized optical morphologies (POM) and identified by powder X-ray diffractions. With the application of the new principle of modular functionalization, local-crosslinked liquid crystalline networks (LCNs) with controlled functionality are successfully synthesized, which show well-controlled phase behaviors over molecular compositions.
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http://dx.doi.org/10.1002/chem.202000268DOI Listing
September 2020

[Gene variant analysis of a patient with multiple carboxylase deficiency].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Apr;37(4):419-422

Department of Medical Genomics, Key Laboratory of Medical Cell Biology of the Ministry of Education, School of Life Science, China Medical University, Shenyang, Liaoning 110122, China.

Objective: To explore the genetic basis for a patient featuring multiple carboxylase deficiency (MCD).

Methods: PCR and Sanger sequencing were used to detect variant in the coding region of BT and HLCS genes in the patient. Suspected variants were verified in her parents and 80 unrelated healthy controls by a PCR-restriction fragment length polymorphism (PCR-RFLP) method.

Results: The patient was found to carry compound heterozygous variants of the HLCS gene, namely c.286delG (p.Val96Leufs*162) and c.1648G>A (p.Val550Met). The c.286delG (p.Val96Leufs*162) was verified to be novel variant based on the result of PCR-RFLP analysis. No variant was found in the coding regions of BT gene in the patient.

Conclusion: The compound c.286delG (p.Val96Leufs*162) and c.1648G>A (p.Val550Met) variants probably underlie the MCD disorder in this patient. Above results have enriched the variant spectrum of MCA.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2020.04.013DOI Listing
April 2020

Corrigendum: An Improved Enzyme-Linked Focus Formation Assay Revealed Baloxavir Acid as a Potential Antiviral Therapeutic Against Hantavirus Infection.

Front Pharmacol 2020 27;11:201. Epub 2020 Feb 27.

Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi'an, China.

[This corrects the article DOI: 10.3389/fphar.2019.01203.].
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http://dx.doi.org/10.3389/fphar.2020.00201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057847PMC
February 2020

Potential contribution of ryanodine receptor 2 upregulation to cGMP/PKG signaling-induced cone degeneration in cyclic nucleotide-gated channel deficiency.

FASEB J 2020 05 16;34(5):6335-6350. Epub 2020 Mar 16.

Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

Photoreceptor cyclic nucleotide-gated (CNG) channels regulate Ca influx in rod and cone photoreceptors. Mutations in cone CNG channel subunits CNGA3 and CNGB3 are associated with achromatopsia and cone dystrophies. Mice lacking functional cone CNG channel show endoplasmic reticulum (ER) stress-associated cone degeneration. The elevated cyclic guanosine monophosphate (cGMP)/cGMP-dependent protein kinase (PKG) signaling and upregulation of the ER Ca channel ryanodine receptor 2 (RyR2) have been implicated in cone degeneration. This work investigates the potential contribution of RyR2 to cGMP/PKG signaling-induced ER stress and cone degeneration. We demonstrated that the expression and activity of RyR2 were highly regulated by cGMP/PKG signaling. Depletion of cGMP by deleting retinal guanylate cyclase 1 or inhibition of PKG using chemical inhibitors suppressed the upregulation of RyR2 in CNG channel deficiency. Depletion of cGMP or deletion of Ryr2 equivalently inhibited unfolded protein response/ER stress, activation of the CCAAT-enhancer-binding protein homologous protein, and activation of the cyclic adenosine monophosphate response element-binding protein, leading to early-onset cone protection. In addition, treatment with cGMP significantly enhanced Ryr2 expression in cultured photoreceptor-derived Weri-Rb1 cells. Findings from this work demonstrate the regulation of cGMP/PKG signaling on RyR2 in the retina and support the role of RyR2 upregulation in cGMP/PKG signaling-induced ER stress and photoreceptor degeneration.
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http://dx.doi.org/10.1096/fj.201901951RRDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299158PMC
May 2020

Vaccines and Therapeutics Against Hantaviruses.

Front Microbiol 2019 30;10:2989. Epub 2020 Jan 30.

Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xi'an, China.

Hantaviruses (HVs) are rodent-transmitted viruses that can cause hantavirus cardiopulmonary syndrome (HCPS) in the Americas and hemorrhagic fever with renal syndrome (HFRS) in Eurasia. Together, these viruses have annually caused approximately 200,000 human infections worldwide in recent years, with a case fatality rate of 5-15% for HFRS and up to 40% for HCPS. There is currently no effective treatment available for either HFRS or HCPS. Only whole virus inactivated vaccines against HTNV or SEOV are licensed for use in the Republic of Korea and China, but the protective efficacies of these vaccines are uncertain. To a large extent, the immune correlates of protection against hantavirus are not known. In this review, we summarized the epidemiology, virology, and pathogenesis of four HFRS-causing viruses, HTNV, SEOV, PUUV, and DOBV, and two HCPS-causing viruses, ANDV and SNV, and then discussed the existing knowledge on vaccines and therapeutics against these diseases. We think that this information will shed light on the rational development of new vaccines and treatments.
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http://dx.doi.org/10.3389/fmicb.2019.02989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002362PMC
January 2020

Preparation of Green Low Strength Mixture for Foundation Reinforcement Treatment by Using Fly Ash and Waste Coal Gangue.

Materials (Basel) 2020 Feb 2;13(3). Epub 2020 Feb 2.

School of Civil Engineering, Central South University, Changsha 410075, China.

Effective foundation reinforcement treatment is essential for modern large and complex infrastructure, while it is significant for developing new green high-performance materials for foundation reinforcement. This study investigates a new green concrete by using high volume fly-ash and coal gangue aggregates, which is expected to apply for foundation treatment of modern infrastructure with high loading-bear ability. In this experiment, 12 mix proportions of fly ash coal gangue mixture (the material name, abbreviated FGM) were designed, and its mechanical properties and durability performance were investigated. The mechanical properties of FGM include compressive strength, dynamic elastic modulus, dynamic shear modulus, Poisson's ratio, and the stress-strain behaviors. The durability performance was evaluated by the parameters of acid resistance, which simulated an acid circumstance. After that, the environmental effects about carbon emission of this material were also investigated. Results show that the FGM with 84.6% wastes utilizing rate is a cost-effective material for foundation reinforcing treatment. Its compressive strength at 28 days and 60 days can reach more than 8 MPa and 10 MPa, respectively. After being immersed in the acid environment for 140 days, the mass loss (%) of the material could be under 3.5%. The greenness shows that the e-CO indices of FGM are lower than 20 kg/MPa·m, and the e-energy indices are at below 150 MJ/MPa·m. FGM has the advantages of acid resistance, waste recycling, and lower carbon emissions than the previous methods for foundation improvement.
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http://dx.doi.org/10.3390/ma13030664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040594PMC
February 2020

Inhibition of thyroid hormone signaling protects retinal pigment epithelium and photoreceptors from cell death in a mouse model of age-related macular degeneration.

Cell Death Dis 2020 01 13;11(1):24. Epub 2020 Jan 13.

Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

Age-related macular degeneration (AMD) is the leading cause of blindness in the elderly. Dry AMD is characterized by a progressive macular degeneration of the retinal pigment epithelium (RPE) and photoreceptors, and the RPE oxidative damage/dystrophy is at the core of the disease. Recent population/patients-based studies have shown an association of high free serum thyroid hormone (TH) levels with increased risk of AMD. This work investigated the effects of TH signaling inhibition on RPE and photoreceptor damage/cell death in an oxidative stress-induced mouse model of AMD. TH signaling inhibition was achieved by anti-thyroid drug treatment and oxidative stress was induced by sodium iodate (NaIO) administration. Mice treated with NaIO showed severe RPE and photoreceptor cell death/necroptosis, destruction, oxidative damage, retinal stress, and reduced retinal function. Treatment with anti-thyroid drug protected RPE and photoreceptors from damage/cell death induced by NaIO, reduced oxidative damage of RPE and photoreceptors, and preserved retinal function. Gene expression analysis showed that the NaIO-induced RPE/photoreceptor damage/cell death involves multiple mechanisms, including cellular oxidative stress responses, activation of necroptosis/apoptosis signaling, and inflammatory responses. Treatment with anti-thyroid drug abolished these cellular stress/death responses. The findings of this study demonstrate a role of TH signaling in RPE and photoreceptor cell death after oxidative stress challenge, and support a role of TH signaling in the pathogenesis of AMD.
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http://dx.doi.org/10.1038/s41419-019-2216-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957507PMC
January 2020

Epitope-Containing Short Peptides Capture Distinct IgG Serodynamics That Enable Differentiating Infected from Vaccinated Animals for Live-Attenuated Vaccines.

J Virol 2020 02 28;94(6). Epub 2020 Feb 28.

Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, China

Differentiating infected from vaccinated animals (DIVA) strategies have been central enabling techniques in several successful viral disease elimination programs. However, owing to their long and uncertain development process, no DIVA-compatible vaccines are available for many important diseases. We report herein a new DIVA strategy based on hybrid protein-peptide microarrays which can theoretically work with any vaccine. Leading from our findings from peste des petits ruminants (PPR) virus, we found 4 epitope-containing short peptides (ECSPs) which have distinct IgG serodynamics: anti-ECSP IgGs only exist for 10 to 60 days postvaccination (dpv), while anti-protein IgGs remained at high levels for >1,000 dpv. These data enabled the design of a DIVA diagnostic microarray containing 4 ECSPs and 3 proteins, which, unlike competitive enzyme-linked immunosorbent assay (cELISA) and virus neutralization tests (VNTs), enables ongoing monitoring of serological differences between vaccinated individuals and individuals exposed to the pathogen. For 25 goats after 60 dpv, 13 were detected with positive anti-ECSP IgGs, indicating recent infections in vaccinated goat herds. These DIVA diagnostic microarrays will almost certainly facilitate eradication programs for (re)emerging pathogens and zoonoses. Outbreaks of infectious diseases caused by viruses, such as pseudorabies (PR), foot-and-mouth disease (FMD), and PPR viruses, led to economic losses reaching billions of dollars. Both PR and FMD were eliminated in several countries via large-scale vaccination programs using DIVA-compatible vaccines, which lack the gE protein and nonstructural proteins, respectively. However, there are still extensive challenges facing the development and deployment of DIVA-compatible vaccines because they are time-consuming and full of uncertainty. Further, the negative marker strategy used for DIVA-compatible vaccines is no longer functional for live-attenuated vaccines. To avoid these disadvantageous scenarios, a new strategy is desired. Here, we made the exciting discovery that different IgG serodynamics can be monitored when using protein-based assays versus arrays comprising ECSPs. This DIVA microarray strategy should, in theory, work for any vaccine.
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http://dx.doi.org/10.1128/JVI.01573-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158722PMC
February 2020

Differently Expressed Genes (DEGs) Relevant to Type 2 Diabetes Mellitus Identification and Pathway Analysis via Integrated Bioinformatics Analysis.

Med Sci Monit 2019 Dec 4;25:9237-9244. Epub 2019 Dec 4.

Department of Endocrinology, Fifth People's Hospital of Jinan, Jinan, Shandong, China (mainland).

BACKGROUND The aim of this study was to evaluate the differently expressed genes (DEGs) relevant to type 2 diabetes mellitus (T2DM) and pathway by performing integrated bioinformatics analysis. MATERIAL AND METHODS The gene expression datasets GSE7014 and GSE29221 were downloaded in GEO database, and DEGs from type 2 diabetes mellitus and normal skeletal muscle tissues were identified. Biological function analysis of the DEGs was enriched by GO and KEEG pathway. A PPI network for the identified DEGs was built using the STRING database. RESULTS Thirty top DEGs were identified from 2 datasets: GSE7014 and GSE29221. Of the 30 top DEGs, 20 were up-regulated and 10 were down-regulated. The 20 up-regulated genes were enriched in regulation of mRNA, protein biding, and phospholipase D signaling pathway. The 10 down-regulated genes were enriched in telomere maintenance via semi-conservative replication, AGE-RAGE signaling pathway in diabetic complications, and insulin resistance pathway. In the PPI network of 20 up-regulated DEGs, there were 40 nodes and 84 edges, with an average node degree of 4.2. For the 10 down-regulated DEGs, we found a total of 30 nodes and 105 edges, with an average node degree of 7.0 and local clustering coefficient of 0.812. Among the 30 DEGs, 10 hub genes (CNOT6L, CNOT6, CNOT1, CNOT7, RQCD1, RFC2, PRIM1, RFC4, RFC5, and RFC1) were also identified through Cytoscape. CONCLUSIONS DEGs of T2DM may play an essential role in disease development and may be potential pathogeneses of T2DM.
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http://dx.doi.org/10.12659/MSM.918407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909911PMC
December 2019

An Improved Enzyme-Linked Focus Formation Assay Revealed Baloxavir Acid as a Potential Antiviral Therapeutic Against Hantavirus Infection.

Front Pharmacol 2019 16;10:1203. Epub 2019 Oct 16.

Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi'an, China.

Hantaviruses, etiologic pathogens responsible for two severe human diseases, exist in areas ranging from Eurasia to America and remain global public health concerns. Conventionally, plaque formation assays have been used for hantavirus titering. However, hantaviruses replicate slowly within cells and produce minimal cytopathic effects, making this technique difficult to master. The improved enzyme-linked immunosorbent assay-based antigen detection method is easier to perform but is still time consuming. Here, we established an enzyme-linked focus formation assay (FFA) for Hantaan virus titering that is twice as fast as traditional assays. Moreover, using this method, we evaluated the effects of favipiravir (T-705) and another influenza virus drug, baloxavir acid (BXA), on hantavirus replication. We found that the endonuclease inhibitor BXA exerted similar anti-hantavirus effects as T-705. Overall, we developed a time-saving method for hantavirus titering and suggest BXA as a potential treatment choice for hantavirus-exposed individuals.
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http://dx.doi.org/10.3389/fphar.2019.01203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6807675PMC
October 2019

Construction of High-Performance, High-Temperature Proton Exchange Membranes through Incorporating SiO Nanoparticles into Novel Cross-linked Polybenzimidazole Networks.

ACS Appl Mater Interfaces 2019 Aug 14;11(34):30735-30746. Epub 2019 Aug 14.

Key Laboratory of High Performance Plastics, Ministry of Education. National & Local Joint Engineering Laboratory for Synthesis Technology of High Performance Polymer. College of Chemistry , Jilin University , 2699 Qianjin Street , Changchun 130012 , P. R. China.

The practical applications of phosphoric acid-doped polybenzimidazole (PA-PBI) as high-temperature proton exchange membranes (HT-PEMs) are mainly limited by their poor dimensional-mechanical stability at high acid doping levels (ADLs) and the leaching of PA from membranes during fuel cell operation. In this work, to overcome these issues, we fabricated novel cross-linked PBI networks with additional imidazole groups by employing a newly synthesized bibenzimidazole-containing dichloro compound as cross-linker and an arylether-type Ph-PBI as matrix. Ph-PBI featured by good solubility under high molecular weight offers satisfactory film-forming ability and mechanical strength using for the matrix. Importantly, the additional imidazole moieties in BIM-2Cl endow the cross-linked PBI membranes improved dimensional-mechanical stability with simultaneously enhanced ADLs and proton conductivity. Furthermore, superior acid retention capability is obtained by incorporating porous polyhydroxy SiO nanoparticles into these cross-linked networks. As a result, the SiO/cross-linked PBI composite membranes are suitable to manufacture membrane electrode assemblies (MEAs), and an excellent H/O cell performance with a peak power density of 497 mW cm at 160 °C under anhydrous conditions can be achieved.
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http://dx.doi.org/10.1021/acsami.9b06808DOI Listing
August 2019

Visualization of Ultrasensitive and Recyclable Dual-Channel Fluorescence Sensors for Chemical Warfare Agents Based on the State Dehybridization of Hybrid Locally Excited and Charge Transfer Materials.

Anal Chem 2019 09 17;91(17):10927-10931. Epub 2019 Jul 17.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry , Jilin University , Changchun 130012 , P. R. China.

Simple and fast detection of chemical warfare agents vapor is necessary and urgent to fight against uncertain terrorist attacks and wars. In this contribution, inspired by the design of the hybrid locally excited and charge transfer (HLCT) excited state, two fast and highly sensitive visualization and fluorescence probes for DCP detection with relative small interstate coupling (J) TPA-2AC and TPA-9AC are reported. Upon exposure to saturated DCP vapor, the TPA-9AC test strips exhibited a rapid fluorescent response in no more than 1 s, accompanied by a change of the color from green to red. The detection limit of the test strips can be estimated as sensitive as 0.15 ppb, which is far superior to the "harmless" level (7 ppb) of human response to acute sarin exposure. More impressively, the fluorescent intensity of the test strips can be quickly restored when exposed to ammonia vapor for cyclic utilization, demonstrating an application prospect in the real-time detection of chemical warfare agents.
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http://dx.doi.org/10.1021/acs.analchem.9b02085DOI Listing
September 2019

4-Aryl Pyrrolidines as Novel Orally Efficacious Antimalarial Agents. Part 2: 2-Aryl--(4-arylpyrrolidin-3-yl)acetamides.

ACS Med Chem Lett 2019 Jun 28;10(6):966-971. Epub 2019 May 28.

Drug Discovery Pipeline at the Guangzhou Institutes for Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.

Malaria is caused by infection from the parasite and kills hundreds of thousands of people every year. Emergence of new drug resistant strains of demands identification of new drugs with novel chemotypes and mechanisms of action. As a follow up to our evaluation of 4-aryl--benzylpyrrolidine-3-carboxamides as novel pyrrolidine-based antimalarial agents, we describe herein the structure-activity relationships of the reversed amide homologues 2-aryl--(4-arylpyrrolidin-3-yl)acetamides. Unlike their carboxamide homologues, acetamide pyrrolidines do not require a third chiral center to be potent inhibitors of . and have good pharmacokinetic properties and improved oral efficacy in a mouse model of malaria. Compound (CWHM-1552) has an IC of 51 nM in the 3D7 assay and an ED of <10 mg/kg/day and ED of 30 mg/kg/day in a murine model. Remarkably, the absolute stereochemical preference for this acetamide series (3,4) is opposite of that determined for the homologous carboxamide series. Lead compounds for this class have modest affinities for the hERG channel and inhibit CYP 3A4. Additional optimization is needed in order to eliminate these undesired properties from this otherwise promising series of antimalarial compounds.
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http://dx.doi.org/10.1021/acsmedchemlett.9b00123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580545PMC
June 2019

Abnormal Thalamic Functional Connectivity During Light Non-Rapid Eye Movement Sleep in Children With Primary Nocturnal Enuresis.

J Am Acad Child Adolesc Psychiatry 2020 05 18;59(5):660-670.e2. Epub 2019 Jun 18.

Shengjing Hospital of China Medical University, Shenyang, China.

Objective: To investigate abnormalities of thalamocortical and intrathalamic functional connectivity (FC) in children with primary nocturnal enuresis (PNE) during light non-rapid eye movement (NREM) sleep using a simultaneous electroencephalography (EEG)-functional magnetic resonance imaging (fMRI) method.

Method: Polysomnographic and EEG-fMRI data were obtained during sleep from 61 children with PNE (age 10.2 ± 1.7 years, 59% boys) and 61 age-matched controls (age 10.1 ± 1.4 years, 54% boys). All subjects first participated in one overnight video-polysomnographic study. Total sleep time, percentage of total sleep time in each sleep stage, arousal index, and awakening index were calculated. Simultaneous EEG-fMRI studies were then performed using a 3T MRI system with a 32-channel MRI-compatible EEG system. Visual scoring of EEG data permitted sleep staging. Thalamocortical and intrathalamic FCs in the waking state and at different stages of light sleep were calculated and compared.

Results: Children with PNE had a higher percentage of total sleep time in light sleep and a higher arousal index compared with controls. Abnormal thalamocortical FCs were detected in the lateral prefrontal cortex, medial prefrontal cortex, and inferior parietal lobule during light NREM sleep. Abnormal intrathalamic FCs were also detected during light NREM sleep among the motor, occipital, prefrontal, and temporal subdivisions of the thalamus.

Conclusion: Abnormal prefrontal and parietal thalamocortical FCs, accompanied by abnormal intrathalamic FCs among the motor, occipital, prefrontal, and temporal subdivision of thalamus during light NREM sleep, may be related to abnormal sleep and enuresis in children with PNE.
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http://dx.doi.org/10.1016/j.jaac.2019.05.028DOI Listing
May 2020

MGMT autoantibodies as a potential prediction of recurrence and treatment response biomarker for glioma patients.

Cancer Med 2019 08 17;8(9):4359-4369. Epub 2019 Jun 17.

Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu.

Background: Cancer-specific autoantibodies found in serum of cancer patients have been characterized as potential predictors of the high risk of recurrence and treatment response. The objective of this study is to investigate the clinical utility of serum O-6-methylguanine-DNA methyltransferase (MGMT) autoantibodies as novel biomarkers for prediction of recurrence and treatment response for glioma through MGMT peptides microarray.

Methods: A total of 201 serum samples of glioma patients with various WHO grade and 311 serum samples of healthy donors were examined for the detection of MGMT autoantibodies by peptides microarray. The clinical value of MGMT autoantibodies was studied through univariable and multivariable analyses.

Results: Autoantibodies to MGMT peptides were detected in sera from glioma patients and five highly responsive autoantibodies to peptides were identified in the glioma group. The positive rate of MGMT autoantibody to 20 peptides in glioma groups is compared with healthy individuals, the positive rate of MGMT-02 (45%), MGMT-04 (27%), MGMT-07 (21%), MGMT-10 (13%), and MGMT-18 (24%) were significantly elevated in patients with glioma. MGMT autoantibody and its protein expression exhibited a significant correlation. The levels of MGMT autoantibodies decreased on the 30th day after operation, reaching preoperative levels, similar to those when tumor recurrence developed. Univariable and multivariable analyses revealed that the only preoperative autoantibodies to MGMT-02 peptide were independently correlated with recurrence-free survival. Preoperative seropositive patients were more likely than seronegative patients to have shorter recurrence times and to be resistant to chemoradiotherapy or chemotherapy with temozolomide.

Conclusion: Monitoring the levels of preoperative serum autoantibodies to MGMT-02 peptide was useful for predicting patients at high risk of recurrence and treatment response.
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http://dx.doi.org/10.1002/cam4.2346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675704PMC
August 2019

Ryanodine Receptor 2 Contributes to Impaired Protein Localization in Cyclic Nucleotide-Gated Channel Deficiency.

eNeuro 2019 May/Jun;6(3). Epub 2019 Jun 27.

Department of Cell Biology, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, Oklahoma 73104

The photoreceptor cyclic nucleotide-gated (CNG) channel plays a pivotal role in phototransduction and cellular calcium homeostasis. Mutations in the cone photoreceptor CNG channel subunits CNGA3 and CNGB3 are associated with achromatopsia and cone dystrophies. CNG channel deficiency leads to endoplasmic reticulum (ER) stress-associated cone apoptosis, protein mislocalization, and ER calcium dysregulation. This work investigated the potential mechanisms of protein mislocalization associated with ER calcium dysregulation using mice lacking ER Ca channel ryanodine receptor 2 (RyR2) specifically in cones. Deletion of improved outer segment (OS) localization of the cone proteins M-opsin, S-opsin, and cone phosphodiesterase subunit α' (PDE6C) and decreased inner segment localization. One-month-old mice showed ∼30% of M-opsin, 55% of S-opsin, and 50% of PDE6C localized to the OS. mice with deletion at the same age showed almost 60% of M-opsin, 70% of S-opsin, and 70% of PDE6C localized to the OS. Deletion of nearly completely reversed elevations of the ER stress markers phospho-IRE1α and phospho-eIF2α and suppressed cone apoptosis. Consistent with the improved cone protein localization and reduced ER stress/cone apoptosis, cone survival was improved by deletion of The number of cones was increased by ∼28% in 2- to 4-month-old mice with deletion compared with age-matched mice. This work demonstrates a role of RyR2/ER calcium dysregulation in protein mislocalization, ER stress, and cone death. The findings provide novel insights into the mechanisms of photoreceptor degeneration and support strategies targeting ER calcium regulation to manage retinal degeneration.
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http://dx.doi.org/10.1523/ENEURO.0119-19.2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597858PMC
March 2020

The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.

Cell Rep 2019 05;27(7):2075-2091.e5

Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. Electronic address:

Hantavirus infection, which causes severe zoonotic diseases with high mortality in humans, has become a global public health concern. Here, we demonstrate that Hantaan virus (HTNV), the prevalent prototype of the hantavirus in Asia, can restrain innate immune responses by manipulating host autophagy flux. HTNV induces complete mitophagy at the early stage of infection but incomplete autophagy at the late stage, and these responses involve the viral glycoprotein (Gn) and nucleocapsid protein (NP), respectively. Gn translocates to mitochondria and interacts with TUFM, recruiting LC3B and promoting mitophagy. Gn-induced mitophagy inhibits type I interferon (IFN) responses by degrading MAVS. Additionally, we found that NP competes with Gn for binding to LC3B, which inhibits Gn-mediated autophagosome formation, and interacts with SNAP29, which prevents autophagosome-lysosome fusion. Thus, NP disturbs the autophagic degradation of Gn. These findings highlight how hantaviruses repurpose host autophagy and evade innate immune responses for their life cycle and pathogenesis.
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http://dx.doi.org/10.1016/j.celrep.2019.04.061DOI Listing
May 2019

Numerical Study on Ultrasonic Guided Waves for the Inspection of Polygonal Drill Pipes.

Sensors (Basel) 2019 May 8;19(9). Epub 2019 May 8.

School of Mechanical Engineering, Xi'an University of Science and Technology, Xi'an 710054, China.

The polygonal drill pipe is one of the most critical yet weakest part in a high-torque drill machine. The inspection of a polygonal drill pipe to avoid its failure and thus to ensure safe operation of the drilling machine is of great importance. However, the current most frequently used ultrasonic inspection method is time-consuming and inefficient when dealing with a polygonal drill pipe, which is normally up to several meters. There is an urgent need to develop an efficient method to inspect polygonal drill pipes. In this paper, an ultrasonic guided wave technique is proposed to inspect polygonal drill pipes. Dispersion curves of polygonal drill pipes are firstly derived by using the semi-analytical finite element method. The ALID (absorbing layer using increasing damping) technique is applied to eliminate unwanted boundary reflections. The propagation characteristics of ultrasonic guided waves in normal, symmetrically damaged, and asymmetrically damaged polygonal drill pipes are studied. The results have shown that the ultrasonic guided wave technique is a promising and effective method for the inspection of polygonal drill pipes.
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http://dx.doi.org/10.3390/s19092128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539063PMC
May 2019