Publications by authors named "Hongwei He"

74 Publications

Nitrogen-carbon materials base on pyrolytic graphene hydrogel for oxygen reduction.

J Colloid Interface Sci 2021 Jun 8;602:274-281. Epub 2021 Jun 8.

Lab of Advanced Nano-structures & Transfer Processes, Department of Chemical Engineering, Tianjin University, Tianjin 300354, China; Chemistry and Chemical Engineering Guangdong Laboratory, Shantou 515031 China. Electronic address:

Hypothesis: Oxygen reduction reaction (ORR) has played a significant role in the utilization of energy nowadays. Nitrogen-doped carbon materials are seen as promising catalysts for ORR, so it is of great significance in studying the functions of different nitrogen moieties.

Experiments: The graphene hydrogel-based nitrogen-arbon materials (GH N-C) were fabricated by first obtaining a gel through hydrothermal treatment using graphene oxide (GO) as precursor, and then calcined in an ammonia atmosphere at different temperatures to form N-doped graphitized materials with divers nitrogen configuration.

Findings: GH N-C materials with tunable nitrogen configuration were synthesized by a two-step method base on graphene hydrogel. Benefiting from the 3D hydrogel structure, rich defects and optimized chemical properties, GH N-C-900 prepared by NH pyrolysis at 900 °C exhibits an excellent electrocatalytic performance toward ORR, with the onset potential of 0.947 ± 0.013 V versus RHE, half-wave potential of 0.830 ± 0.010 V versus RHE, electron transfer number of 3.61-3.99, along as methanol tolerance and superior long-term stability. Comprehensive studies have shown that there is a positive correlation between the total amount of pyrrolic-N and quaternary-N and the catalytic performance of ORR.
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http://dx.doi.org/10.1016/j.jcis.2021.06.036DOI Listing
June 2021

Correlation of periodontal diseases with intracranial aneurysm formation: novel predictive indicators.

Chin Neurosurg J 2021 Jun 7;7(1):31. Epub 2021 Jun 7.

Department of Interventional Neuroradiology, Beijing Neurosurgical Institute and Beijing Tiantan Hospital, Capital Medical University, No. 119, West Road of South Fourth Ring, Fengtai, Beijing, 100070, People's Republic of China.

Background: We investigated whether periodontal diseases, specifically, periodontitis and gingivitis, could be risk factors of the incidence of intracranial aneurysms (IAs).

Methods: We performed a case-control study to compare the differences in the periodontal disease parameters of 281 cases that were divided into the IAs group and non-IAs group. All cases underwent complete radiographic examination for IAs and examination for periodontal health.

Results: Comparing with those in the non-IAs group, the cases in the IAs group were older (53.95 ± 8.56 vs 47.79 ± 12.33, p < 0.001) and had a higher incidence of hypertension (76 vs 34, p = 0.006). Univariate logistic regression analysis revealed that age (> 50 years) and hypertension were predictive risk factors of aneurysm formation (odds ratio [OR] 1.047, 95% confidence interval [95% CI] 1.022-1.073, p < 0.001 and OR 2.047, 95% CI 1.232-3.401, p = 0.006). In addition, univariate and multivariate logistic regression analyses showed that the parameters of periodontal diseases, including gingival index, plaque index, clinical attachment loss, and alveolar bone loss, were significantly associated with the occurrence of IAs (all p < 0.05). For further statistical investigation, the parameters of periodontal diseases were divided into four layers based on the quartered data. Poorer periodontal health condition (especially gingival index > 1.1 and plaque index > 1.5) had the correlation with IAs formation (p = 0.007 and p < 0.001).

Conclusion: Severe gingivitis or periodontitis, combining with hypertension, is significantly associated with the incidence of IAs.
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http://dx.doi.org/10.1186/s41016-021-00249-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182916PMC
June 2021

Bimetallic ZIF-Derived Co/N-Codoped Porous Carbon Supported Ruthenium Catalysts for Highly Efficient Hydrogen Evolution Reaction.

Nanomaterials (Basel) 2021 May 6;11(5). Epub 2021 May 6.

Lab of Advanced Nano-Structure and Transfer Process, Department of Chemical Engineering, Tianjin University, Tianjin 300354, China.

Exploring the economical, powerful, and durable electrocatalysts for hydrogen evolution reaction (HER) is highly required for practical application. Herein, nanoclusters-decorated ruthenium, cobalt nanoparticles, and nitrogen codoped porous carbon ([email protected]) are prepared with bimetallic zeolite imidazole frameworks (ZnCo-ZIF) as the precursor. Thus, the prepared [email protected] catalyst with a low Ru loading of 3.13 wt% exhibits impressive HER catalytic behavior in 1 M KOH, with an overpotential of only 30 mV at the current density of 10 mA cm, Tafel slope as low as 32.1 mV dec, and superior stability for long-time running with a commercial 20 wt% Pt/C. The excellent electrocatalytic properties are primarily by virtue of the highly specific surface area and porosity of carbon support, uniformly dispersed Ru active species, and rapid reaction kinetics of the interaction between Ru and O.
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http://dx.doi.org/10.3390/nano11051228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148513PMC
May 2021

Radiosurgery-Based AVM Scale Is Proposed for Combined Embolization and Gamma Knife Surgery for Brain Arteriovenous Malformations.

Front Neurol 2021 30;12:647167. Epub 2021 Mar 30.

Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.

To evaluate whether a radiosurgery-based arteriovenous malformation (AVM) scale (RBAS) could be used to predict obliteration of brain arteriovenous malformations (bAVMs) supposed for combined endovascular embolization (EMB) and gamma knife surgery (GKS) treatment. bAVM patients who underwent GKS with or without previous EMB from January 2011 to December 2016 at our institution were retrospectively reviewed. The patients were categorized into a combined treatment group and a GKS group. A 1:1 propensity score matching (PSM) was used to match the two groups. Pre-EMB and pre-GKS RBAS were assessed for every patient. Multivariate analysis was performed to find factors associated with complete obliteration in the combined treatment group. Survival analysis based on sub-groups according to RBAS was performed to compare obliteration rate and find cutoffs for appropriate treatment modalities. A total of 96 patients were involved, and each group comprised 48 patients. There was no difference between the two groups in terms of obliteration rate (75.0 vs. 83.3%, = 0.174). Pre-EMB RBAS ( = 0.010) and the number of feeding arteries ( = 0.014) were independent factors associated with obliteration rate in the combined treatment group. For the combined treatment patients, sub-group analysis according to pre-EMB RBAS (score <1.0, 1.0-1.5, and >1.5) showed statistical difference in obliteration rate ( = 0.002). Sub-group analysis according to RBAS between the two groups showed that the obliteration rate of the GKS group is significantly higher than the combined group when RBAS >1.5 (47.4 vs. 66.7%, = 0.036). The RBAS is proposed to be efficient in predicting obliteration of bAVMs supposed to receive combined EMB and GKS treatment. Patients with RBAS >1.5 are inclined to be more suitable for GKS instead of the combined treatment.
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http://dx.doi.org/10.3389/fneur.2021.647167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042217PMC
March 2021

SOX9 Knockdown-Mediated FOXO3 Downregulation Confers Neuroprotection Against Ischemic Brain Injury.

Front Cell Dev Biol 2020 12;8:555175. Epub 2021 Mar 12.

Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Evidence exists uncovering that SRY-box transcription factor 9 (SOX9) plays a role in ischemic brain injury (IBI). Thus, the current study was conducted to elucidate the specific role of SOX9 and the mechanism by which SOX9 influenced IBI.

Methods: The IBI-associated regulatory factors were searched by bioinformatics analysis. The rat model of IBI was generated using middle cerebral artery occlusion (MCAO) treatment. Neuronal cells were exposed to oxygen-glucose deprivation (OGD). The expressions of SOX9, forkhead box O3 (FOXO3), transcription of Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (CITED2), and IκB kinase α (IKKα) in OGD-treated neuronal cells were characterized using reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay. The interaction among CITED2, IKKα, and FOXO3 was identified by chromatin immunoprecipitation (ChIP) and dual luciferase reporter gene assays. Gain- and loss-of-function experiments were performed to verify the relationship among SOX9, FOXO3, CITED2, and IKKα and to investigate their functional effects on apoptosis and the inflammatory response of OGD-treated neuronal cells as well as neurological deficit and infarct area of the rat brain.

Results: SOX9, FOXO3, CITED2, and IKKα were highly expressed in OGD-treated neuronal cells. Silencing of SOX9 inhibited OGD-induced neuronal apoptosis and inflammatory response and reduced the neurological deficit and infarct area of the brain in the rats, which were caused by MCAO but were reversed by overexpressing FOXO3, CITED2, or IKKα.

Conclusion: Taken together, our study suggested that upregulation of SOX9 promoted IBI though upregulation of the FOXO3/CITED2/IKKα axis, highlighting a basic therapeutic consideration for IBI treatment.
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http://dx.doi.org/10.3389/fcell.2020.555175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006459PMC
March 2021

Computed tomography comparison study of two Axis-based C1 transpedicular screw trajectory designs.

Spine J 2021 Mar 20. Epub 2021 Mar 20.

Department of Radiology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:

Background Context: To our knowledge, there is no comparison study of two different Axis-based C1 transpedicular screw trajectory (TST) designs.

Purpose: To compare two different Axis-based C1 TST designs.

Study Design: The computed tomography (CT) morphologic analysis of the two different C1 Axis-based TST designs.

Methods: Firstly, the design of Axis C/M and related measurements were made on a work station by using neck computed tomography angiography (CTA) data of 62 patients. The central axes (Axis M and Axis C) of C1 TST were designed by multiplanar reconstruction (MPR) technique. Based on Axis M and Axis C, the following parameters were measured: (A, A'), distance between the insertion point (IP) and the midline. (B, B'), distance between IP and the inferior aspect of C1 posterior arch. (C, C'), distance between IP and the C1 anterior cortex of the lateral mass along Axis M/C. (D, D'), insertion angle (IA) based on Axis M/C. (E, E'), the narrowest width of the inner medullary cavity (IMC) along Axis M/C.

Result: The C1 TST can be designed by MPR technique based on CT volume scan. The design of Axis C trajectory was easier to Axis M trajectory. A, A' were 20.7±1.6mm and 20.6±1.9mm. Both B, B' were 2.0±0.6mm. C, C' were 27.2±2.1mm and 27.5±2.1mm. D, D' were 9.9±5.0°and 10.4±7.7°. E, E' were 5.0±1.3mm and 5.4±1.4mm. Among 62 patients (124 sides), Axis C can be achieved by medial inclination, perpendicular, lateral inclination method in 81.5%, 13.7%, 4.8% sides, respectively; Axis M can be achieved by medial inclination, perpendicular, lateral inclination method in 90.3%, 8.9%, 0.8% sides, respectively.

Conclusion: Axis C can be a reliable trajectory for C1 transpedicular screw insertion as long as there is no displaced fracture in the C1 pedicle and lateral mass. Because of the individual differences, the ideal C1 TST can be achieved by medial inclination, perpendicular or even lateral inclination method, although its inclination direction is medial in the majority of patients.
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http://dx.doi.org/10.1016/j.spinee.2021.03.013DOI Listing
March 2021

Simultaneous detection of diethylstilbestrol and estradiol residues with a single immunochromatographic assay strip.

Food Sci Nutr 2021 Mar 20;9(3):1824-1830. Epub 2021 Jan 20.

Tianjin Institute of Environmental and Operational Medicine Tianjin China.

An immunochromatographic assay (ICA) based on competitive format was developed and validated for simultaneously rapid and sensitive detection of diethylstilbestrol (DES) and estradiol (E) in milk and tissue samples. For this purpose, two monoclonal antibodies raised against those two estrogens were conjugated to gold nanoparticles and were applied to the conjugate pads of the test strip. The competitors of the DES-BSA/E-BSA conjugates were immobilized onto a nitrocellulose membrane at two detection zones to form T and T, respectively. The immunochromatographic assay had a visual detection limit of DES at 30 ng/g in milk powder, 25 ng/g in liquid milk, and 25 ng/g in shrimp tissue, respectively, and the results can be judged within 7-10 min. The visual detection limit of E was 75 ng/g in milk powder, 65 ng/g in liquid milk, and 60 ng/g in shrimp tissue, respectively, and the results can be judged within 3-4 min. It had advantages in easy operation without requiring sophisticated equipment and specialized skills. By testing thirty milk and shrimp tissue samples from the local market, the method was compared with the HPLC-MS / MS method, and there was no statistical difference between the two methods. Furthermore, the immunochromatographic assay had good specificity, simple procedure, and low cost. This protocol was well suited for the food safety monitoring and early warning.
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http://dx.doi.org/10.1002/fsn3.2127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958558PMC
March 2021

Synergistic Effect of N-Doped sp Carbon and Porous Structure in Graphene Gels toward Selective Oxidation of C-H Bond.

ACS Appl Mater Interfaces 2021 Mar 11;13(11):13087-13096. Epub 2021 Mar 11.

School of Chemical Engineering and Technology, State Key Laboratory of Chemical Engineering, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, People's Republic of China.

N-doped carbon materials represent a type of metal-free catalyst for diverse organic synthetic reactions. However, single N-doped carbon materials perform insufficiently in the selective oxidation reaction of C-H bond compared with metal catalysts or multielement co-doped materials. There are a few reports on the application of three-dimensional (3D) carbon materials in such a reaction. Besides, the relationship between the well-developed porous structures, heteroatom doping, and their catalytic performance is unclear. In this study, 3D porous N-doped graphene aerogel catalysts with high activity and selectivity for the C-H bond oxidation under mild reaction conditions have been synthesized through a two-step method. Systematic studies on the dosage of N sources, pyrolysis temperature, and their influences on the catalytic performances have been evolved. Moreover, solid evidence of the synergistic effect of sp C atoms adjacent to the N atoms and porous structure promoting the performance has been provided in this work.
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http://dx.doi.org/10.1021/acsami.0c21177DOI Listing
March 2021

Down-regulation of EOMES drives T-cell exhaustion via abolishing EOMES-mediated repression of inhibitory receptors of T cells in liver cancer.

J Cell Mol Med 2021 Jan 16;25(1):161-169. Epub 2020 Dec 16.

Key Laboratory for Carcinogenesis and Cancer Invasion, Liver Cancer Institute, Zhongshan Hospital and Shanghai Medical School, Fudan University, Shanghai, China.

T-cell exhaustion is one of the hallmarks in cancer, but the mechanisms underlying T-cell dysregulation remains unclear. Here, we reported that down-regulation of transcription factor EOMES contributed to increased levels of inhibitory receptors in T cell among the tumour tissues and resulted in the poor prognosis of hepatocellular carcinoma (HCC). By analysing the correlation between EOMES in tumour-infiltrating T cells and the clinical features, we demonstrated that the EOMES was related to the advanced stage and poor prognosis of HCC. Further mechanistic studies revealed that the EOMES mainly expressed in the CD8 T cells and were down-regulated in tumour samples. Moreover, we demonstrated that the EOMES directly bound at the transcriptional regulatory regions of the key inhibitory factors including PD-1, CTAL-4 and CD39, and lower levels of EOMES contributed to overexpression of these factors in T cells. Together, our studies provide new insight into the transcriptional deregulation of the inhibitory receptors on T cells during the tumorigenesis.
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http://dx.doi.org/10.1111/jcmm.15898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810931PMC
January 2021

Construction of ceRNA network and identification of two differentially expressed circRNAs in hepatocellular carcinoma by bioinformatic analysis.

Int J Clin Exp Pathol 2020 1;13(11):2727-2737. Epub 2020 Nov 1.

Department of General Surgery, Jinshan Branch of The Sixth People's Hospital of Shanghai China.

Covalently closed circular RNAs (circRNAs) display dysregulated expression in several types of cancer. However, their functions remain largely unclear. In this work, datasets GSE125469 and GSE128274 of hepatocellular carcinoma (HCC) were selected from Gene Expression Omnibus (GEO) database. To identify differentially expressed genes (DEGs) in HCC and adjacent tissues, we used R package DESeq for analysis. Then, 15 DEcircRNAs, 65 DEmiRNAs, and 2084 DEmRNAs were identified comparing HCC and normal tissues. Next, to predict the target relationship of circRNA-miRNA and miRNA-mRNA in DEGs, we use the databases CircInteractome and starBase v2.0 for analysis. Finally, the ceRNA network of circRNA-miRNA-mRNA was established by Cytoscape software based on 2 DEcircRNAs (hsa_circ_0007813 and hsa_circ_0089372), 2 DEmiRNAs, and 98 DEmRNAs. In addition, we conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of DEGs to explore the function of DEGs in HCC. Functional enrichment analyses indicated DEmRNAs might be associated with HCC occurrence and progression. In general, our research reveals an important role of ceRNA's molecular mechanism in HCC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716122PMC
November 2020

Structure-Activity Relationship of Aloperine Derivatives as New Anti-Liver Fibrogenic Agents.

Molecules 2020 Oct 27;25(21). Epub 2020 Oct 27.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Twenty-seven novel 12-substituted aloperine derivatives were synthesized and investigated for their inhibitory effects on collagen α1 (I) (COL1A1) promotor in human hepatic stellate LX-2 cells, taking aloperine () as the hit. A structure-activity relationship (SAR) study disclosed that the introduction of suitable substituents on the 12 atom might enhance the activity. Compound exhibited a good promise on down-regulating COL1A1 expression with the IC value of 16.5 μM. Its inhibitory activity against COL1A1 was further confirmed on both mRNA and protein levels. Meanwhile, it effectively inhibited the expression of other fibrogenic proteins, such as transforming growth factor β1 (TGF-β1) and smooth muscle actin (α-SMA). It also exhibited good in vivo safety profile with the oral LD value of 400 mg kg in mice. The results initiated the anti-liver fibrogenic study of aloperine derivatives, and the key compound was selected as a novel lead for further investigation against liver fibrogenesis.
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http://dx.doi.org/10.3390/molecules25214977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663597PMC
October 2020

Aminated N-doped graphene hydrogel for long-term catalytic oxidation in strong acidic environment.

J Hazard Mater 2021 Jan 22;401:123742. Epub 2020 Aug 22.

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300050, China. Electronic address:

Metal-based catalysts in advanced oxidation processes (AOPs) are not stable under strong acidic condition due to the remarkable leaching, which will also lead to a secondary pollution. In this study, an aminated N-doped graphene hydrogel (ANGH) is synthesized from graphene oxide and ethylenediamine (EDA) via an in-situ hydrothermal process. The ANGH shows a free-standing structure and has high catalytic activity especially in phenol degradation under strong-acidic condition because of a non-radical dominated mechanism determined in this process. On the large scale, a longer lifetime of ∼1700 min for ANGH is obtained under strong-acidic condition on a dynamic amplifying device, 2.9 times longer than that at neutral condition. It is proposed that amine N can be protected by hydrogen ions from being oxidized, thus leading to the better stability. Meanwhile, the active sites of ANGH can transform from N containing groups into oxygenous groups, and the deactivated material can be reutilized 10 times for rhodamine B degradation on a large scale. The ANGH synthesized facilely and could be recycled repeatedly, which is also very stable in the strong acidic environment, thus should have great potential in wastewater remediation.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123742DOI Listing
January 2021

Complete antigen-bridged DNA strand displacement amplification immuno-PCR assay for ultrasensitive detection of salbutamol.

Sci Total Environ 2020 Dec 9;748:142330. Epub 2020 Sep 9.

Tianjin Institute Environmental and Operational Medicine, Tianjin 300050, China. Electronic address:

Monitoring of low-level analytes are typical examples for analytical challenges. Salbutamol (SAL), a phenol-β2-agonist, has a very low residual content in the environment. Here, we present an ultrasensitive complete antigen-bridged PCR assay for detecting salbutamol (SAL). These DNA probes modified SAL complete antigens target recognition SAL antibodies and agglutinate synthetic DNA conjugates, thus enabling ligation of DNA probes to form a full-length DNA amplicon that contained a recognition site for cleavage endonuclease and subsequent quantification by qPCR. Moreover, SAL antibodies were modified with magnetic beads which were used to reduce the background noise and sample matrix effect, and the DNA signals were isothermally amplified by strand displacement amplification technology. Some key parameters which influence assay performance were optimized: the length of the bridge oligonucleotide, the concentration of immunomagnetic beads, SAL probes, and initiation chain, etc. Under the optimum conditions, the signal amplification of proposed Immuno-PCR assay for the detection of SAL was exponential, resulting in high potential sensitivity(~1 fg/mL) and a broad detection dynamic range (> 10 fold). Using this proposed method, we detected SAL in spiked tap water and urine samples with acceptable recoveries ranging from 88.1 to 103.3%. Theoretically, the method developed here has broad applicability and practical utility in immunoassays of a wide variety of analytes.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142330DOI Listing
December 2020

Raw and wine processed Schisandra chinensis attenuate anxiety like behavior via modulating gut microbiota and lipid metabolism pathway.

J Ethnopharmacol 2021 Feb 29;266:113426. Epub 2020 Sep 29.

Laboratory Animal Science and Technology Center, College of Pharmacy, College of Science and Technology, Jiangxi University of Traditional Chinese Medicine, 1688 Meiling Road, Nanchang, 330004, PR China. Electronic address:

Ethnopharmacological Relevance: In traditional Chinese medicine, the fruit of Schisandra chinensis (Turcz.) Baill (SC) is used to treat various nervous system diseases, such as dysphoria, anxiety, insomnia and many dreams. It is worthy to be noted that wine processed Schisandra chinensis (WSC) has been applied in clinic for thousands of years.

Aim Of Study: This study aimed to investigate the possible mechanism and related metabolism of SC and WSC ameliorating anxiety behavior through modulating gut microbiota.

Materials And Methods: The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used for the quality control of chemical components in SC and WSC. Chronic unpredictable stress procedure (CUSP)-induced anxiety rats were administrated with SC and WSC via gavage for five weeks. An untargeted UPLC/LTQ-Orbitrap MS metabolomic analysis of plasma was conducted to understand the effects of long-term intake of WSC and SC extracts on anxious rats. 16S rRNA microbial sequencing technology was applied to investigate gut microbiota structure. Expression of GPR81, TNF-α, S1PR2 as well as molecules in cAMP pathway was assayed by immunohistochemistry staining, RT-qPCR, or Western blot, respectively.

Results: 12 compounds were identified using UPLC-QTOF-MS technology, all of which are lignans. Results demonstrate that the amounts of 6-O-Benzoylgomisin O, Schisandrin, Gomisin D, Schizandrin A, Gomisin T, Schizandrin B, Schisandrin C were higher in wine-processed samples than in raw samples. Furthermore, both SC and WSC significantly ameliorated anxiety- and depression-like behavior and lipid metabolism dysfunction and attenuated hippocampal neuritis in anxiety rats. After WSC treatment, the structure and composition of gut microbiota in anxiety rats changed significantly, and gut microbiota derivatives lactate level was significantly lower in the plasma and feces. WSC treatment help restore gut microbial ecosystem dysbiosis and reverse the changes in Lachnospiraceae, Lactobacillus, Alloprevotella, and Bacteroidales in anxiety rat. In addition, the expression of liver GPR81 was decreased, and the molecules in cAMP pathway were increased in SC and WSC-treated anxiety rat.

Conclusion: Raw and wine processed Schisandra chinensis treatment improved anxiety- and depression-like behavior through modulating gut microbiota derivatives in association with GPR81 receptor-mediated lipid metabolism pathway. And WSC has more exhibition than SC.
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http://dx.doi.org/10.1016/j.jep.2020.113426DOI Listing
February 2021

The impact of Covid-19 pandemic on corporate social responsibility and marketing philosophy.

J Bus Res 2020 Aug 21;116:176-182. Epub 2020 May 21.

Birmingham Business School, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.

In this article, we offer some initial examination on how Covid-19 pandemic can influence the developments of CSR and marketing. We argue that Covid-19 pandemic offers a great opportunity for businesses to shift towards more genuine and authentic CSR and contribute to address urgent global social and environmental challenges. We also discuss some potential directions of how consumer ethical decision making will be shifted to due to the pandemic. In our discussion of marketing, we outline how we believe marketing is being affected by this pandemic and how we think this will change, not only the context of marketing, but how organizations approach their strategic marketing efforts. We end the paper with a identifying a number of potentially fruitful research themes and directions.
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http://dx.doi.org/10.1016/j.jbusres.2020.05.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241379PMC
August 2020

Development of sandwich chemiluminescent immunoassay based on an anti-staphylococcal enterotoxin B Nanobody-Alkaline phosphatase fusion protein for detection of staphylococcal enterotoxin B.

Anal Chim Acta 2020 Apr 22;1108:28-36. Epub 2020 Jan 22.

Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment & Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, China. Electronic address:

In this study, sandwich chemiluminescent immunoassay (CLIA) for the detection of Staphylococcal enterotoxin B (SEB) was developed using nanobody-alkaline phosphatase (Nb-ALP) fusion protein. The SEB-binding nanobodies were obtained from a naïve phage-display library and the Nb-ALP fusion protein was constructed and obtained as a thermally stable and potentially effective substance for detecting antibodies in CLIA. The working range of the sandwich CLIA based on anti-SEB monoclonal antibodies (mAbs) and our fusion protein, Nb37-ALP, was 3.12-50.0 ng mL with SC50 = 8.59 ± 0.37 ng mL. The limit of detection was 1.44 ng mL according to the blank value plus 3 standard deviations. In order to understand the interaction of SEB and Nb37 in depth, the 3D structure of the SEB-Nb37 complex was constructed and verified by molecular modeling and the docking method. The results showed that the complementary-determining region 3 (CDR3) of Nb37 embedded itself in the opening generated by the major histocompatibility complex (MHC) and T-cell receptor- (TcR) binding sites of SEB, indicating that Nb37 may affect the recognition of SEB by MHC class Ⅱ molecules and the TcR. The arginine residue (Arg) 101, Arg102 and phenylalanine residue (Phe)103 of CDR3 in Nb37 may have contributed to specific binding to form six salt-bridges between these and SEB. In conclusion, in terms of their specificity and sensitivity, the obtained anti-SEB Nb-ALP appears to have the potential to replace chemically labeled probes for the detection of SEB.
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http://dx.doi.org/10.1016/j.aca.2020.01.032DOI Listing
April 2020

Discovery of 9O-Substituted Palmatine Derivatives as a New Class of anti-COL1A1 Agents via Repressing TGF-β1/Smads and JAK1/STAT3 Pathways.

Molecules 2020 Feb 11;25(4). Epub 2020 Feb 11.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Twenty 9O-substituted palmatine derivatives were prepared and tested for their biological effect against collagen α1 (I) (COL1A1) promotor in human hepatic stellate LX-2 cells. The structure-activity relationship (SAR) indicated that the introduction of a benzyl motif on the 9O atom was favorable for activity. Among them, compound 6c provided the highest inhibitory effect against COL1A1 with an IC value of 3.98 μM, and it also dose-dependently inhibited the expression of fibrogenic COL1A1, α-soomth muscle actin (α-SMA), matrix metalloprotein 2 (MMP2) in both mRNA and protein levels, indicating extensive inhibitory activity against fibrogenesis. A further primary mechanism study indicated that it might repress the hepatic fibrogenesis via inhibiting both canonical transforming growth factor-beta 1 (TGF-β1)/Smads and non-canonical janus-activated kinase 1 (JAK1)/singal transducer and activator of transcription 3 (STAT3) signaling pathways. Additionally, c owned a high safety profile with the LD value of over 1000 mg·kg in mice. These results identified palmatine derivatives as a novel class of anti-fibrogenic agents, and provided powerful information for further structure optimization.
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http://dx.doi.org/10.3390/molecules25040773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070350PMC
February 2020

Mn/Ce Oxides Decorated Polyphenylene Sulfide Needle-Punching Fibrous Felts for Dust Removal and Denitration Application.

Polymers (Basel) 2020 Jan 9;12(1). Epub 2020 Jan 9.

Industrial Research Institute of Nonwovens & Technical Textiles, College of Textiles & Clothing, Qingdao University, Qingdao 266071, China.

Development of a novel filter material is urgently required for replacing the high-cost flue gas purification technology in the simultaneous removal of both fine dust and Nitrogen oxides (NO). In this study; polyphenylene sulfide (PPS) needle-punching fibrous felts (NPFF) were employed as the filter material to remove the fine dust; and in the meanwhile; Mn and Ce oxides were loaded onto the PPS NPFF as the catalyst for selective catalytic reduction of NO with NH. Two different pretreatment methods; i.e., sodium alginate (SA) deposition and plasma treatment; were employed to modify the PPS NPFF before the traditional impregnation and thermal treatment processes during the catalyst loading. The results showed that these two pretreatment methods both afforded the PPS NPFF with the enhanced loading rate and stability of Mn/Ce oxides compared to those without any pretreatments; which were significantly beneficial for the denitration application. Moreover; we found that both SA deposition and plasma pre-treated samples presented excellent dust-removal properties; and the filtration efficiency could reach 100% when the particle size of the fine particulates was above 4 μm. This study demonstrated that our Mn/Ce oxides decorated PPS NPFF have great potential to be applied in the fuel gas purification field; due to their stable structure; handling convenience; and excellent filtration efficiency; as well as high denitration performance.
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http://dx.doi.org/10.3390/polym12010168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022841PMC
January 2020

Insight into the interaction between arabinoxylan and imidazolium acetate-based ionic liquids.

Carbohydr Polym 2020 Mar 30;231:115699. Epub 2019 Nov 30.

Center of Analysis and Testing, Nanchang University, Nanchang, Jiangxi 330047, PR China.

Herein, six ionic liquids (ILs) with different cations and the same anion of acetate (Ac) were used to dissolve arabinoxylan. These ILs included N-methylimidazolium acetate (HmimAc), 1-ethyl-3-methylimidazolium acetate (EmimAc), 1-hydroxyethyl-3-methylimidazolium acetate (HOemimAc), 1-propyl-3-methylimidazolium acetate (PrmimAc), 1-allyl-3-methylimidazolium acetate (AmimAc), and 1-butyl-3-methylimidazolium acetate (BmimAc). The solubilities of arabinoxylan in these ILs were determined, and the dissolution mechanism was explained using H and C NMR techniques. The solubilities of arabinoxylan in the ILs were in the order: BmimAc > EmimAc > AmimAc > PrmimAc > HOemimAc > HmimAc. Both the cation and Ac played an important role in the solubilization of arabinoxylan, but Ac performed the major factor. The structure of cation greatly affected the hydrogen bond accepting ability of Ac. Increasing the mass ratio of arabinoxylan to ILs resulted in stronger hydrogen bond between arabinoxylan and the ILs. Both the solubility and the strength of hydrogen-bonding interaction between arabinoxylan and the ILs decreased in the recycled ILs because of the impurities remained.
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http://dx.doi.org/10.1016/j.carbpol.2019.115699DOI Listing
March 2020

Nano-Crystalline Sandwich Formed in Polylactic Acid Fibers.

Macromol Rapid Commun 2019 Dec 6;40(23):e1900492. Epub 2019 Nov 6.

Industrial Research Institute of Nonwovens & Technical Textiles, College of Textiles & Clothing, Qingdao University, Qingdao, 266071, China.

Fibers have traditionally been made through melt or solution processes from macromolecules. Most of these fibers have crystalline domains where the segregation of different crystalline features is extremely difficult due to the statistical nature of the formation and growth of these domains. A fibrous nano-crystalline sandwich is reported where distinctly different crystalline regions are formed in layers along the continuous fiber direction during the spinning process and locked in place. This approach employs side-by-side bicomponent nanofiber electrospinning where the components are the enantiomeric pair of poly(l-lactic acid) and poly(d-lactic acid). The formation of the poly(lactic acid) (PLA) stereo-complexes at the junction interphase of the two components is demonstrated through diffusion, which subsequently crystallize into continuous sandwich domains. The stereo-complex crystalline core in the fiber possesses a melting point 50 °C higher than, and properties substantially different from, the regular PLAs at the fringe areas of the fiber. This nano-crystalline sandwich fiber structure can be scaled to the micrometers in a commercial bicomponent process.
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http://dx.doi.org/10.1002/marc.201900492DOI Listing
December 2019

12N-Substituted Matrinol Derivatives Inhibited the Expression of Fibrogenic Genes via Repressing Integrin/FAK/PI3K/Akt Pathway in Hepatic Stellate Cells.

Molecules 2019 Oct 17;24(20). Epub 2019 Oct 17.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Twenty new 12N-substituted matrinol derivatives were synthesized and evaluated for their inhibitory effects on collagen α1 (I) (COL1A1) promotor in human hepatic stellate LX-2 cells. The structure-activity relationship (SAR) revealed that introducing a 12N-benzeneaminoacylmethyl substitution might significantly enhance the activity. Compound 8a exhibited the highest inhibitory potency against COL1A1, and its inhibition activity against COL1A1 was further confirmed on both the mRNA and protein levels. It also effectively inhibited the expression of α smooth muscle actin (α-SMA), fibronectin and transforming growth factor β1 (TGFβ1), indicating an extensive inhibitory effect on the expression of fibrogenic genes. The primary mechanism study indicated that it might take action via the Integrin/FAK/PI3K/Akt signaling pathway. The results provided powerful information for further structure optimization, and compound 8a was selected as a novel anti-fibrogenic lead for further investigation.
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http://dx.doi.org/10.3390/molecules24203748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832694PMC
October 2019

Bifunctional Graphene-Based Metal-Free Catalysts for Oxidative Coupling of Amines.

ACS Appl Mater Interfaces 2019 Sep 20;11(35):31844-31850. Epub 2019 Aug 20.

Lab of Advanced Nano Structures & Transfer Processes, Department of Chemical Engineering , Tianjin University , Tianjin 300354 , P. R. China.

Graphene oxide (GO), an emerging material ornamented with oxygen-containing functional groups, is becoming a promising alternative for various applications. The piranha solution treatment of GO can increase oxygen-containing functional groups and result in improved graphene oxide (IGO), as well as restore the functional groups lost because of the reaction. It is found that GO can oxidize the amine to the corresponding imine in the absence of oxygen and a catalyst, and the obtained IGO even shows higher activity. In addition, the piranha solution can partially restore the reactivity of GO after the reaction. The different roles of oxygen-containing functional groups in the oxidative coupling reaction are investigated. A possible reaction mechanism for the oxidation of benzylamine to -benzylidene benzylamine is also proposed.
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http://dx.doi.org/10.1021/acsami.9b08741DOI Listing
September 2019

Novel cytisine derivatives exert anti-liver fibrosis effect via PI3K/Akt/Smad pathway.

Bioorg Chem 2019 09 8;90:103032. Epub 2019 Jun 8.

Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address:

A series of new cytisine derivatives with a unique endocyclic scaffold were synthesized and evaluated for their inhibitory effect on collagen α1 (I) (COL1A1) promotor in human LX2 cells, taking cytisine as the lead. Structure-activity relationship (SAR) revealed that introducing a 12N-benzyl substitution might significantly enhance the activity. Compound 5f exhibited a promising inhibitory potency against COL1A1 with an IC value of 12.8 μM in human LX2 cells, and an inspiring inhibition activity against COL1A1 on both mRNA and protein levels. It also effectively inhibited the expression of α smooth muscle actin (α-SMA), connective tissue growth factor (CTGA), matrix metalloprotein 2 (MMP-2), and transforming growth factor β1 (TGFβ1), indicating an extensive inhibitory effect against fibrogenetic proteins. In addition, compound 5f displayed reasonable PK and safety profiles. The primary mechanism study indicated that it might repress the hepatic fibrogenesis via PI3K/Akt/Smad signaling pathway. The results provided powerful information for further structure optimization, and compound 5f was selected as a novel anti-liver fibrosis agent for further investigation.
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http://dx.doi.org/10.1016/j.bioorg.2019.103032DOI Listing
September 2019

[Automatic Segmentation of Anatomical Areas in X-ray Images Based on Fully Convolutional Networks].

Zhongguo Yi Liao Qi Xie Za Zhi 2019 May;43(3):170-172

Modern Educational Technology Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, 271016.

Objective: Medical image segmentation is a key step in medical image processing. An architecture of fully convolutional networks was proposed to realize automatic segmentation of anatomical areas in X-ray images.

Methods: Enlightened by the advantages of convolutional neural networks on features extraction, fully convolutional networks consisting of 9 layers were designed to segment medical images. The networks used convolution kernels of various sizes to extract multi-dimensional image features in the images, meanwhile, eliminated pooling layers to avoid the loss of image details during downsampling procedures.

Results: The experiment was conducted in accordance with the specific scene of X-ray images segmentation. Compared with traditional segmentation methods, this approach achieved more accurate segmentation of anatomical areas.

Conclusions: Fully convolutional networks can extract representative and multidimensional features of medical images, avoid the loss of image details during downsampling procedures, and complete automatic segmentation of anatomical areas accurately in X-ray images.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2019.03.004DOI Listing
May 2019

Association of Thrombelastographic Parameters with Complications in Patients with Intracranial Aneurysm After Stent Placement.

World Neurosurg 2019 Jul 18;127:e30-e38. Epub 2019 Feb 18.

Department of Interventional Neuroradiology, Beijing Neurosurgical Institute and Beijing Tiantan Hospital, Capital Medical University, Beijing, China; Beijing Engineering Research Center for Interventional Neuroradiology, Beijing, China. Electronic address:

Objective: A prospective trial was conducted to investigate the platelet function and association between thrombelastographic (TEG) parameters and embolic or hemorrhagic complications in patients with intracranial aneurysm undergoing stent treatment.

Methods: Between September 2013 and June 2016, we prospectively recruited patients with intracranial aneurysm who were treated with stent-assisted coiling. TEG parameters were used to assess the platelet function before stenting procedures. The primary study end point was the onset of ischemic stroke, transient ischemic attack, or silent ischemic events in the territory of the stented artery within 6 months after the procedure. The secondary end point was assessed by bleeding events.

Results: Four hundred and thirty-one patients with 453 intracranial aneurysms were enrolled. A total of 519 neurovascular stents were implanted. During the follow-up, a total of 70 primary end points (16.2%) and 59 secondary end points (13.7%) were detected. Thromboembolic complications such as symptomatic and slient ischemic complications were more frequently observed in patients with large aneurysms (>10 mm, P = 0.01), lower adenosine diphosphate (ADP) inhibition rate (P < 0.0001), and higher ADP-induced platelet-fibrin clot strength (maximum amplitude of adenosine diphosphate [MA-ADP]) (P < 0.0001). Besides, based on multivariate analysis, a higher ADP inhibition ratio was identified as a significant independent predictor of subsequent bleeding events (P < 0.0001). According to the receiver operating characteristic curve analysis, the safe range of the ADP inhibition ratio and MA-ADP of the TEG analysis were identified as 29.45%-55.4% and <46.15, respectively.

Conclusions: The ADP inhibition ratio and MA-ADP of TEG analysis were associated with subsequent cerebral ischemic events and intracranial or extracranial bleeding events in patients with intracranial aneurysm after stent treatment.
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http://dx.doi.org/10.1016/j.wneu.2019.02.007DOI Listing
July 2019

Efficient Synthesis of PVDF/PI Side-by-Side Bicomponent Nanofiber Membrane with Enhanced Mechanical Strength and Good Thermal Stability.

Nanomaterials (Basel) 2018 Dec 29;9(1). Epub 2018 Dec 29.

Industrial Research Institute of Nonwovens & Technical Textiles, College of Textiles & Clothing, Qingdao University, Qingdao 266071, China.

Bicomponent composite fibers, due to their unique versatility, have attracted great attention in many fields, such as filtration, energy, and bioengineering. Herein, we efficiently fabricated polyvinylidene fluoride/polyimide (PVDF/PI) side-by-side bicomponent nanofibers based on electrospinning, which resulted in the synergism between PVDF and PI, and eventually obtained the effect of 1 + 1 > 2. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) were used to characterize the morphology and chemical structure of nanofibers, indicating that a large number of side-by-side nanofibers were successfully prepared. Further, the thermal stability, mechanical strength, and filtration properties of PVDF/PI were carefully investigated. The results revealed that the bicomponent nanofibers possessed both good mechanical strength and remarkable thermal stability. Moreover, the mechanical properties of PVDF/ PI were strengthened by more than twice after the heat treatment (7.28 MPa at 25 °C, 15.49 MPa at 230 °C). Simultaneously, after the heat treatment at 230 °C for 30 min, the filtration efficiency of PVDF/PI membrane was maintained at about 95.45 ± 1.09%, and the pressure drop was relatively low. Therefore, the prepared PVDF/PI side-by-side bicomponent nanofibers have a favorable prospect of application in the field of medium- and high-temperature filtration, which further expands the application range of electrospun fiber membranes.
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http://dx.doi.org/10.3390/nano9010039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359095PMC
December 2018

3D self-supported Ni(PO)-MoO nanorods anchored on nickel foam for highly efficient overall water splitting.

Nanoscale 2018 Dec 27;10(47):22173-22179. Epub 2018 Nov 27.

Lab of Advanced Nano Structures & Transfer Processes, Department of Chemical Engineering, Tianjin University, Tianjin 300354, P. R. China.

Electrolyzing water as a sustainable energy source is a promising and appealing method to resolve the environmental crisis. Developing efficient and stable bifunctional electrocatalysts for the hydrogen evolution reaction (HER) and the oxygen evolution reaction (OER) is crucial and challenging in the overall water splitting process. Herein, we report the synthesis of Ni(PO)-MoO nanorods anchored on nickel foam (Ni(PO)-MoO/NF) within a two-step strategy and their application as a bifunctional water splitting electrocatalyst. The results show that the optimal Ni(PO)-MoO/NF electrodes exhibit superior catalytic activity with robust durability and ultralow overpotentials of 86 mV for HER and 234 mV for OER to achieve 10 mA cm (η) in alkaline solution. The favorable performance of the obtained catalyst is attributed mainly to the synergetic effect between Ni(PO) and MoO, as well as the self-supporting porous conductive substrate. As a result, the integrated Ni(PO)-MoO/NF electrodes deliver η at a small potential of 1.47 V for overall water splitting, highlighting a promising application as a bifunctional electrocatalyst.
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http://dx.doi.org/10.1039/c8nr07577hDOI Listing
December 2018

Electrospinning of Ultrafine Conducting Polymer Composite Nanofibers with Diameter Less than 70 nm as High Sensitive Gas Sensor.

Materials (Basel) 2018 Sep 17;11(9). Epub 2018 Sep 17.

Collaborative Innovation Center for Nanomaterials & Devices, College of Physics, Qingdao University, Qingdao 266071, China.

Polyvinyl alcohol/poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PVA/PEDOT:PSS) composite ultrafine fibers were successfully fabricated by high pressure airflow assisted electrospinning. The electrical properties of PVA/PEDOT:PSS nanofibers with different diameters were characterized. The average diameter of the nanofibers can be down to 68 nm. Due to its large specific surface area, ammonia sensing of the ultrafine nanofibers is more sensitive than the traditional electrospun fibers (average fiber diameter of 263 nm). The ammonia sensing properties of the samples were tested by impedance analysis. The results show that ultrafine PVA/PEDOT:PSS nanofibers are more suitable for detecting low concentrations of ammonia with higher sensitivity.
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http://dx.doi.org/10.3390/ma11091744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164846PMC
September 2018

Quinohemanine, a quinoxalinone-bohemamine hybrid compound from Streptomyces sp. CPCC 200497.

J Antibiot (Tokyo) 2018 11 29;71(11):965-967. Epub 2018 Aug 29.

Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, People's Republic of China.

A quinoxalinone-bohemamine hybrid compound quinohemanine (1), together with 1-methyl-2(H)-quinoxalin-2-one (2), was isolated from Streptomyces sp. CPCC 200497, a producer of quinomycins and bohemamines. Compounds 1 and 2 were purified using standard chromatographic methods, and their structures were defined through interpretation of HRMS, 1D, and 2D NMR data. Both 1 and 2 displayed moderate cytotoxicity against cancer cell line HepG2.
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http://dx.doi.org/10.1038/s41429-018-0084-5DOI Listing
November 2018

Discovery of Matrinic Thiadiazole Derivatives as a Novel Family of Anti-Liver Fibrosis Agents via Repression of the TGFβ/Smad Pathway.

Molecules 2018 07 5;23(7). Epub 2018 Jul 5.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

A series of novel matrinic thiadiazole derivatives were designed, synthesized and evaluated for their inhibitory effect on COL1A1 promotor. The SAR indicated that: (i) the introduction of a thiadiazole on the 11-side chain was beneficial for activity; (ii) a 12--benzyl moiety was favorable for activity. Among them, compound displayed a high activity with an inhibitory rate of 39.7% at a concentration of 40 μM. It also effectively inhibited the expression of two representative collagen proteins (COL1A1 and α-SMA) on both the mRNA and protein levels and showed a high safety profile in vivo, indicating its great promise as an anti-liver fibrosis agent. Further study indicated that it might repress hepatic fibrogenesis via the TGFβ/Smad pathway. This study provided powerful information for further strategic optimization and the top compound was selected for further study as an ideal liver fibrosis lead for next investigation.
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http://dx.doi.org/10.3390/molecules23071644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099493PMC
July 2018
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