Publications by authors named "Hongwei Han"

83 Publications

Enhanced efficiency of printable mesoscopic perovskite solar cells using ionic liquid additives.

Chem Commun (Camb) 2021 Apr;57(33):4027-4030

Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China.

The crystallization mechanism of the perovskite crystals inside the mesopores of printable mesoscopic perovskite solar cells is demonstrated by in situ grazing-incidence X-ray diffraction. Ionic liquids can universally tune the crystallization process of different perovksites in the mesopores regradless of the precursor solvents, resulting in enhanced efficiency.
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http://dx.doi.org/10.1039/d1cc00169hDOI Listing
April 2021

Design, synthesis and biological evaluation of anilide (dicarboxylic acid) shikonin esters as antitumor agents through targeting PI3K/Akt/mTOR signaling pathway.

Bioorg Chem 2021 Mar 29;111:104872. Epub 2021 Mar 29.

State Key Laboratory of Pharmaceutical Biotechnology, Institute of Plant Molecular Biology, School of Life Sciences, Nanjing University, Nanjing 210023, China; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China. Electronic address:

Triple-negative breast cancer (TNBC) has an unfavorable prognosis attribute to its low differentiation, rapid proliferation and high distant metastasis rate. PI3K/Akt/mTOR as an intracellular signaling pathway plays a key role in the cell proliferation, migration, invasion, metabolism and regeneration. In this work, we designed and synthesized a series of anilide (dicarboxylic acid) shikonin esters targeting PI3K/Akt/mTOR signaling pathway, and assessed their antitumor effects. Through three rounds of screening by computer-aided drug design method (CADD), we preliminarily obtained sixteen novel anilide (dicarboxylic acid) shikonin esters and identified them as excellent compounds. CCK-8 assay results demonstrated that compound M9 exhibited better antiproliferative activities against MDA-MB-231, A549 and HeLa cell lines than shikonin (SK), especially for MDA-MB-231 (M9: IC = 4.52 ± 0.28 μM; SK: IC = 7.62 ± 0.26 μM). Moreover, the antiproliferative activity of M9 was better than that of paclitaxel. Further pharmacological studies showed that M9 could induce apoptosis of MDA-MB-231 cells and arrest the cell cycle in G2/M phase. M9 also inhibited the migration of MDA-MB-231 cells by inhibiting Wnt/β-catenin signaling pathway. In addition, western blot results showed that M9 could inhibit cell proliferation and migration by down-regulating PI3K/Akt/mTOR signaling pathway. Finally, a three-dimensional quantitative structure-activity relationship (3D-QSAR) model was also constructed to provide a basis for further development of shikonin derivatives as potential antitumor drugs through structure-activity relationship analysis. To sum up, M9 could be a potential candidate for TNBC therapy.
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http://dx.doi.org/10.1016/j.bioorg.2021.104872DOI Listing
March 2021

Risk Signature Related to Immunotherapy Reaction of Hepatocellular Carcinoma Based on the Immune-Related Genes Associated With CD8 T Cell Infiltration.

Front Mol Biosci 2021 19;8:602227. Epub 2021 Mar 19.

Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Hepatocellular carcinoma (HCC) is the most common histological type of liver cancer, with an unsatisfactory long-term survival rate. Despite immune checkpoint inhibitors for HCC have got glories in recent clinical trials, the relatively low response rate is still a thorny problem. Therefore, there is an urgent need to screen biomarkers of HCC to predict the prognosis and efficacy of immunotherapy. Gene expression profiles of HCC were retrieved from TCGA, GEO, and ICGC databases while the immune-related genes (IRGs) were retrieved from the ImmPort database. CIBERSORT and WGCNA algorithms were combined to identify the gene module most related to CD8 T cells in the GEO cohort. Subsequently, the genes in hub modules were subjected to univariate, LASSO, and multivariate Cox regression analyses in the TCGA cohort to develop a risk signature. Afterward, the accuracy of the risk signature was validated by the ICGC cohort, and its relationships with CD8 T cell infiltration and PDL1 expression were explored. Nine IRGs were finally incorporated into a risk signature. Patients in the high-risk group had a poorer prognosis than those in the low-risk group. Confirmed by TCGA and ICGC cohorts, the risk signature possessed a relatively high accuracy. Additionally, the risk signature was demonstrated as an independent prognostic factor and closely related to the CD8 T cell infiltration and PDL1 expression. A risk signature was constructed to predict the prognosis of HCC patients and detect patients who may have a higher positive response rate to immune checkpoint inhibitors.
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http://dx.doi.org/10.3389/fmolb.2021.602227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017194PMC
March 2021

Score for predicting overall survival in pancreatic adenocarcinoma patients with positive lymph nodes after surgery: a novel nomogram-based risk assessment.

Gland Surg 2021 Feb;10(2):529-540

Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: Pancreatic adenocarcinoma (PaC) patients with positive lymph nodes (PLNs) have a dismal prognosis and lack a specific prognostic stage. This study aimed to construct a nomogram for the prediction of overall survival (OS) in these patients.

Methods: A total of 1,340 patients screened from the Surveillance, Epidemiology, and End Results database were included and randomly divided at a ratio of 7:3 into a training set (n=940) and an internal validation set (n=400). Cox regression analyses were conducted to select independent predictors in the training set, and a nomogram was constructed. The model was verified in the internal validation set and in an external validation set, which comprised 64 patients from a Chinese institute.

Results: Six independent prognostic factors (age at diagnosis, tumor grade, lymph node ratio, T stage, radiotherapy, and chemotherapy) were identified in PaC patients with PLNs and were entered into the nomogram. The final model had a higher C-index for predicting OS than the American Joint Committee on Cancer-8th edition staging system (training set: 0.658 . 0.546; internal validation set: 0.661 . 0.546; external validation set: 0.691 . 0.581). The 1-, 2-, and 3-year area under the receiver operating characteristic curve values indicated better discrimination power for the established nomogram with respect to the prediction of OS in the training, internal validation, and external validation sets than for the American Joint Committee on Cancer-8th edition staging system. Furthermore, the nomogram performed well in both calibration and decision curve analyses (DCA) of clinical applicability. OS in PaC patients with PLNs was significantly distinguished among the three risk groups stratified according to the nomogram score (P<0.001).

Conclusions: The well-calibrated nomogram was determined to be extremely efficient in predicting survival, and defining a high-risk population based on the nomogram score among PaC patients with PLNs after surgery.
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http://dx.doi.org/10.21037/gs-20-597DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944054PMC
February 2021

RNA Sequencing Uncovers Antifibrotic Genes during Lung Fibrosis Resolution.

Am J Respir Cell Mol Biol 2021 04;64(4):401-402

Department of Medicine Fibrosis Research Center and.

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http://dx.doi.org/10.1165/rcmb.2021-0008EDDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008808PMC
April 2021

Differential relieving effects of shikonin and its derivatives on inflammation and mucosal barrier damage caused by ulcerative colitis.

PeerJ 2021 7;9:e10675. Epub 2021 Jan 7.

State Key Laboratory of Pharmaceutical Biotechnology, Institute of Plant Molecular Biology, School of Life Sciences, Nanjing University, Nanjing, China.

Background: Ulcerative colitis (UC) is one of the most challenging human diseases. Natural shikonin (SK) and its derivatives (with have higher accumulation) isolated from the root of have numerous beneficial effects, such as wound healing and anti-inflammatory activities. Some researchers have reported that hydroxynaphthoquinone mixture (HM) and SK attenuate the acute UC induced by dextran sulfate sodium (DSS). However, no existing study has systemically investigated the effectiveness of SK and other hydroxynaphthoquinone natural derivative monomers on UC.

Methods: In this study, mice were treated with SK and its derivatives (25 mg/kg) and mesalazine (200 mg/kg) after DSS administration daily for one week. Disease progression was monitored daily by observing the changes in clinical signs and body weight.

Results: Intragastric administration natural single naphthoquinone attenuated the malignant symptoms induced by DSS. SK or its derivatives remarkably suppressed the serum levels of pro-inflammatory cytokines while increasing the inflammatory cytokine interleukin (IL)-10 . Additionally, both SK and alkanin restrained the activities of cyclooxygenase-2 (COX-2), myeloperoxidase (MPO) and inducible nitric oxide synthase (iNOS) in serum and colonic tissues. SK and its derivatives inhibited the activation of nucleotide binding oligomerization domain-like receptors (NLRP3) inflammasome and NF-κB signaling pathway, thereby relieving the DSS-induced disruption of epithelial tight junction (TJ) in colonic tissues.

Conclusions: Our findings shed more lights on the pharmacological efficacy of SK and its derivatives in UC against inflammation and mucosal barrier damage.
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http://dx.doi.org/10.7717/peerj.10675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797173PMC
January 2021

Construction of Nomograms for Predicting Lung and Bone Metastases in Patients with Intrahepatic Cholangiocarcinoma and Identification of Patients Who Can Benefit from Chemotherapy.

J Oncol 2020 2;2020:8889571. Epub 2020 Dec 2.

Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Objective: The purpose of our study is to build nomograms for predicting the possibility of lung metastasis (LM) and bone metastasis (BM) in patients with intrahepatic cholangiocarcinoma (ICC).

Methods: 1527 patients diagnosed with ICC between 2010 and 2016 were collected from the Surveillance, Epidemiology, and End Results (SEER) database. Univariable and multivariable logistic regression analyses were used to recognize the predictors of LM and BM, respectively. Then two nomograms were established. We applied the C-index, calibration plot, receiver-operating characteristic (ROC) curve, and decision curve analysis (DCA) to evaluate the novel nomograms. The maximum values of the Youden indexes from the ROC curves were utilized to select the cutoff points of the nomograms. The Kaplan-Meier survival curves were used to evaluate the effect of chemotherapy in different groups. The bootstrap resampling method was chosen for internal validation.

Results: Five predictors for LM and three predictors for BM were identified, and two nomograms were constructed. The nomograms had high values of C-indexes, reaching 0.821 (95% CI 0.772-0.871) for LM and 0.759 (95% CI 0.700-0.818) for BM. C-indexes of 0.814 for LM and 0.749 for BM were also observed in internal validation. The calibration plots, ROC curves, and DCAs exhibited favorable performances for predicting LM and BM. The cutoff points of total points in nomograms were 108 for LM and 144 for BM, which could distinguish between high-risk and low-risk groups for LM and BM. Chemotherapy is suggested to undergo for patients in high-risk groups.

Conclusions: The nomograms could assess the possibility of LM and BM in ICC patients and determine the optimal treatment.
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http://dx.doi.org/10.1155/2020/8889571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725572PMC
December 2020

CCDC170 affects breast cancer apoptosis through IRE1 pathway.

Aging (Albany NY) 2020 12 3;13(1):1332-1356. Epub 2020 Dec 3.

Department of Epidemiology and Biostatistics, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.

Genome-wide association studies have revealed that multiple single-nucleotide polymorphisms in the intergenic region between estrogen receptor 1 and coiled-coil domain containing 170 () are associated with breast cancer risk. We performed microarray and bioinformatics analyses to identify genes that were induced upon CCDC170 overexpression, and confirmed our findings by evaluating paraffin-embedded breast cancer tissues and conducting cellular assays. In CCDC170-overexpressing MCF7 breast cancer cells, microarray analyses revealed that inositol-requiring enzyme 1 () was the most elevated gene in enriched pathways. In breast cancer tissues, IRE1 expression correlated positively with CCDC170 and X-box binding protein 1 expression at both the mRNA and protein levels. In a survival analysis, patients with higher CCDC170 levels exhibited better disease-free survival. Western blotting indicated that overexpressing CCDC170 in MCF7 cells increased protein levels of IRE1α, estrogen receptor α and X-box binding protein 1, while silencing CCDC170 reduced them. CCDC170 overexpression promoted apoptosis in MCF7 cells, and this effect was more obvious under endoplasmic reticulum stress. MCF7 cells overexpressing CCDC170 were more sensitive to paclitaxel. Our study showed that higher CCDC170 expression is associated with a better prognosis in breast cancer patients and that CCDC170 may promote apoptosis through the IRE1α pathway.
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http://dx.doi.org/10.18632/aging.202315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835043PMC
December 2020

The role of hyperthermic intraperitoneal chemotherapy in the treatment of spontaneously ruptured hepatocellular carcinoma: a pilot study.

Ann Transl Med 2020 Sep;8(18):1132

Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: Spontaneous tumor rupture is a distinctive disease pattern in patients with hepatocellular carcinoma (HCC). The application of hyperthermic intraperitoneal chemotherapy (HIPEC) in spontaneously ruptured hepatocellular carcinoma (srHCC) is debatable. Our study aimed to compare the long-term outcomes of srHCC nrHCC and to test the role of postoperative HIPEC in patients with srHCC after hepatectomy.

Methods: From 2014 to 2018, PSM was performed to compare 57 patients who performed liver resection for srHCC and met the research criteria with 57 nrHCC patients selected from 446 consecutive patients. Then patients with srHCC were divided into two groups according to whether they underwent HIPEC after hepatectomy.

Results: After 1:1 PSM, the clinical characteristics of the patients with srHCC and nrHCC were comparable. In terms of long-term outcomes, the nrHCC group had significantly longer OS (P=0.026) and DFS (P<0.001) than the srHCC group. Of the 57 srHCC patients, the HIPEC group showed added complications compared to the non-HIPEC group, including an increased length of hospital stay and higher in-hospital costs. However, there were no significant differences in the metastatic patterns of these recurrent patients, and there was no statistically significant difference in DFS (P=0.28) or OS (P=0.56) between the two groups.

Conclusions: The prognosis of ruptured HCC patients were worse than those of non-ruptured HCC patients. HIPEC may not be a robust treatment for srHCC now.
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http://dx.doi.org/10.21037/atm-20-5829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576033PMC
September 2020

Pyruvate Kinase M2 Coordinates Metabolism Switch between Glycolysis and Glutaminolysis in Cancer Cells.

iScience 2020 Nov 15;23(11):101684. Epub 2020 Oct 15.

Department of Biology, Georgia State University, 145 Piedmont Ave SE, Atlanta, GA 30303, USA.

Cancer cells alter their nutrition metabolism to cope the stressful environment. One important metabolism adjustment is that cancer cells activate glutaminolysis in response to the reduced carbon from glucose entering into the TCA cycle due to inactivation of several enzymes in glycolysis. An important question is how the cancer cells coordinate the changes of glycolysis and glutaminolysis. In this report, we demonstrate that the pyruvate kinase inactive dimer PKM2 facilitates activation of glutaminolysis. Our experiments show that growth stimulations promote PKM2 dimer. The dimer PKM2 plays a role in regulation of glutaminolysis by upregulation of mitochondrial glutaminase I (GLS-1). PKM2 dimer regulates the GLS-1 expression by controlling internal ribosome entry site (IRES)-dependent c-myc translation. Growth stimulations promote PKM2 interacting with c-myc IRES-RNA, thus facilitating c-myc IRES-dependent translation. Our study reveals an important linker that coordinates the metabolism adjustment in cancer cells.
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http://dx.doi.org/10.1016/j.isci.2020.101684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644948PMC
November 2020

Development of a Nomogram to Predict Disease-Specific Survival for Patients After Resection of a Non-Metastatic Adenocarcinoma of the Pancreatic Body and Tail.

Front Oncol 2020 29;10:526602. Epub 2020 Oct 29.

Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: Models for predicting patient survival after resection of a non-metastatic adenocarcinoma of the pancreatic body and tail (APBT) are scarce. We wished to establish and validate a nomogram to predict disease-specific survival (DSS) of these patients.

Methods: A total of 1,435 patients screened from the Surveillance, Epidemiology, and End Results (SEER) database were included and divided randomly into a training set (TS; = 1,007) and internal validation set (IVS; = 428) at a ratio of 7:3. Cox regression analyses were conducted to select independent predictors in the TS, and a nomogram was constructed. The model was subjected to the IVS and an external validation set (EVS) comprising 151 patients from two tertiary hospitals.

Results: Five independent risk factors (age at the diagnosis, chemotherapy, tumor grade, T stage, and the lymph node radio) were identified and integrated into the nomogram. Calibration curves indicated that the nomogram could predict DSS at 1, 2, and 3 years accurately. The nomogram had a higher concordance index for predicting DSS compared with that using the 8th edition of the American Joint 23 Committee on Cancer (AJCC8) stage (TS: 0.681 . 0.606; IVS: 0.662 . 0.590; and EVS: 0.675 . 0.608). The nomogram had better discrimination ability and clinical utility than the AJCC8 stage for predicting 1-, 2-, and 3-year DSS.

Conclusion: Our developed nomogram could accurately predict DSS in patients after resection of a non-metastatic APBT.
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http://dx.doi.org/10.3389/fonc.2020.526602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658586PMC
October 2020

Mesoporous-Carbon-Based Fully-Printable All-Inorganic Monoclinic CsPbBr Perovskite Solar Cells with Ultrastability under High Temperature and High Humidity.

J Phys Chem Lett 2020 Nov 2;11(22):9689-9695. Epub 2020 Nov 2.

Michael Grätzel Center for Mesoscopic Solar Cells (MGC), Wuhan National Laboratory for Optoelectronics (WNLO) Huazhong University of Science and Technology (HUST), Wuhan 430074, China.

The all-inorganic CsPb(IBr) (0 ≤ ≤ 1) perovskite solar cells (PSCs) are attractive by virtue of their high environmental and thermal stability. Nevertheless, multiple-step deposition and high annealing temperature (>250 °C) and the structural and optoelectronic properties changes upon temperature-dependent phase-transition are potential impediments for highly efficient and stable PSCs. Herein, a space-confined method to fabricate stable lower-order symmetric pure monoclinic CsPbBr phase at low temperature (<50 °C) is for the first time reported. It is found that the carbon-based mesoporous fully printable area can inhibit the phase transition to get a pure phase. Therefore, the device exhibits a power conversion efficiency of 7.52% with a low hysteresis index of 0.024. Moreover, the device passed the 1000 h 85 °C thermal test and the 200 cycles thermal cycling test according to IEC-61625 stability tests. These are critical progresses for achieving long-term stability and the stable pure inorganic perovskite phase of high-performance photovoltaics.
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http://dx.doi.org/10.1021/acs.jpclett.0c02739DOI Listing
November 2020

van der Waals Mixed Valence Tin Oxides for Perovskite Solar Cells as UV-Stable Electron Transport Materials.

Nano Lett 2020 Nov 30;20(11):8178-8184. Epub 2020 Oct 30.

Michael Grätzel Center for Mesoscopic Solar Cells, Wuhan National Laboratory for Optoelectronics, Key Laboratory of Materials Chemistry for Energy Conversion and Storage of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, Hubei, P.R. China.

Stable electron transport materials (ETMs) with fewer surface defects and proper energy level alignments with halide perovskite active layers are required for efficient perovskite solar cells (PSCs) with long-term durability. Here, two-dimensional van der Waals mixed valence tin oxides SnO and SnO are controllably synthesized and applied as ETMs for planar PSCs. The synthesized SnO and SnO have size of 5-20 nm and disperse well in water as stable colloids for months. Both SnO and SnO exhibit typical n-type semiconductor energy band structures, low trap density, and suitable energy level alignments with halide perovskites. Steady-state power conversion efficiencies (PCEs) of 22.36% and 21.83% are obtained for SnO-based and SnO-based planar PSCs. In addition, the half cells without hole transport materials and back electrodes show good UV-stability with average PCE of 99.0% and 95.7% for SnO-based and SnO-based devices remaining after 1000 h of ultraviolet soaking with an intensity of 70 mW cm.
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http://dx.doi.org/10.1021/acs.nanolett.0c03286DOI Listing
November 2020

CDK1, CCNB1, and CCNB2 are Prognostic Biomarkers and Correlated with Immune Infiltration in Hepatocellular Carcinoma.

Med Sci Monit 2020 Aug 31;26:e925289. Epub 2020 Aug 31.

Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China (mainland).

BACKGROUND Orderly G2/M transition in the cell cycle is controlled by the cyclin-dependent kinase 1/cyclin B (CDK1/CCNB) complex. We aimed to comprehensively investigate the roles of CDK1, CCNB1, and CCNB2 via multi-omics analysis and their relationships with immune infiltration in hepatocellular carcinoma (HCC). MATERIAL AND METHODS The transcriptional data and the epigenetic and genetic alterations of CDK1, CCNB1, and CCNB2, as well as their impacts on prognosis in HCC patients, were identified using multiple databases. The correlations between expression of these genes and immune infiltration in HCC were then explored using the TIMER database. RESULTS Overall, mRNA expression of CDK1, CCNB1, and CCNB2 was up-regulated in various tumor tissues including HCC. Higher expression of these genes was associated with poorer prognosis in HCC patients. Lower promoter methylation of these genes might cause higher expression levels in tumor tissues of HCC. Genetic alterations and several methylated-CpG sites in these genes were significantly associated with survival. Notably, expression levels of CDK1, CCNB1, and CCNB2 were positively correlated with infiltrating levels of CD4⁺ T cells, CD8⁺ T cells, neutrophils, macrophages, and dendritic cells in HCC. In addition, significant correlations between the expression of these genes and various immune markers in HCC, such as PD-1, PDL-1, and CTLA-4, were also observed. CONCLUSIONS CDK1, CCNB1, and CCNB2 are potential prognostic biomarkers and associated with immune cell infiltration in HCC. The genes may be utilized to predict the reaction of immunotherapy. Combining inhibitors of these genes with immunotherapy may improve the survival time of HCC patients.
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http://dx.doi.org/10.12659/MSM.925289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482506PMC
August 2020

Single-Molecule Real-Time Transcript Sequencing of Turnips Unveiling the Complexity of the Turnip Transcriptome.

G3 (Bethesda) 2020 10 5;10(10):3505-3514. Epub 2020 Oct 5.

Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, China

To generate the full-length transcriptome of Xinjiang green and purple turnips, , using single-molecule real-time (SMRT) sequencing. The samples of two varieties of at five developmental stages were collected and combined to perform SMRT sequencing. Meanwhile, next generation sequencing was performed to correct SMRT sequencing data. A series of analyses were performed to investigate the transcript structure. Finally, the obtained transcripts were mapped to the genome of Chiifu to identify potential novel transcripts. For green turnip (F01), a total of 19.54 Gb clean data were obtained from 8 cells. The number of reads of insert (ROI) and full-length non-chimeric (FLNC) reads were 510,137 and 267,666. In addition, 82,640 consensus isoforms were obtained in the isoform sequences clustering, of which 69,480 were high-quality, and 13,160 low-quality sequences were corrected using Illumina RNA seq data. For purple turnip (F02), there were 20.41 Gb clean data, 552,829 ROIs, and 274,915 FLNC sequences. A total of 93,775 consensus isoforms were obtained, of which 78,798 were high-quality, and the 14,977 low-quality sequences were corrected. Following the removal of redundant sequences, there were 46,516 and 49,429 non-redundant transcripts for F01 and F02, respectively; 7,774 and 9,385 alternative splicing events were predicted for F01 and F02; 63,890 simple sequence repeats, 59,460 complete coding sequences, and 535 long-non coding RNAs were predicted. Moreover, 5,194 and 5,369 novel transcripts were identified by mapping to Chiifu. The obtained transcriptome data may improve turnip genome annotation and facilitate further study of the genome and transcriptome.
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http://dx.doi.org/10.1534/g3.120.401434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534443PMC
October 2020

Well Logging Based Lithology Identification Model Establishment Under Data Drift: A Transfer Learning Method.

Sensors (Basel) 2020 Jun 29;20(13). Epub 2020 Jun 29.

Department of Automation, University of Science and Technology of China, Hefei 230027, China.

Recent years have witnessed the development of the applications of machine learning technologies to well logging-based lithology identification. Most of the existing work assumes that the well loggings gathered from different wells share the same probability distribution; however, the variations in sedimentary environment and well-logging technique might cause the data drift problem; i.e., data of different wells have different probability distributions. Therefore, the model trained on old wells does not perform well in predicting the lithologies in newly-coming wells, which motivates us to propose a transfer learning method named the data drift joint adaptation extreme learning machine (DDJA-ELM) to increase the accuracy of the old model applying to new wells. In such a method, three key points, i.e., the project mean maximum mean discrepancy, joint distribution domain adaptation, and manifold regularization, are incorporated into extreme learning machine. As found experimentally in multiple wells in Jiyang Depression, Bohai Bay Basin, DDJA-ELM could significantly increase the accuracy of an old model when identifying the lithologies in new wells.
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http://dx.doi.org/10.3390/s20133643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374305PMC
June 2020

transfer of CHNHPbI single crystals in mesoporous scaffolds for efficient perovskite solar cells.

Chem Sci 2020 Jan 20;11(2):474-481. Epub 2019 Nov 20.

Michael Grätzel Center for Mesoscopic Solar Cells , Wuhan National Laboratory for Optoelectronics , China-EU Institute for Clean and Renewable Energy , Huazhong University of Science and Technology , Wuhan 430074 , Hubei , P. R. China . Email: ; Email:

Printable mesoscopic perovskite solar cells are usually fabricated by drop-casting perovskite precursor solution on a screen-printed mesoporous TiO/ZrO/carbon triple-layer followed by thermal annealing. They have attracted much attention due to their simple fabrication process and remarkable stability. However, challenges lie in how to achieve complete pore fillings of perovskites in the meso-pores and to obtain high-quality perovskite crystals. Here, we report an crystal transfer (ICT) process based on gas-solid interaction to deposit perovskite CHNHPbI absorber in the scaffold. CHNHPbI single crystals are first transformed into a liquid phase exposure to methylamine gas flow. After complete infiltration into the nano-structured scaffolds, the liquid phase is converted back to the solid phase with reduction of methylamine gas partial pressure, maintaining the high-quality of CHNHPbI single crystals. Compared with the conventional drop-casting method, the ICT method effectively leads to interconnected morphology and prolongs the charge-carrier lifetime (from ∼37.52 ns to ∼110.85 ns) of the perovskite absorber in the scaffold. As a result, the devices can deliver a power conversion efficiency of 15.89%, which is attributed to the suppressed charge recombination and correspondingly enhanced open-circuit voltage of 0.98 V.
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http://dx.doi.org/10.1039/c9sc04900bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067259PMC
January 2020

Peri-left bundle branch pacing after atrioventricular node ablation and failed his bundle pacing in atrial fibrillation.

J Arrhythm 2020 Feb 8;36(1):203-205. Epub 2020 Jan 8.

Department of Cardiovascular Medicine Wuhan Asia Heart Hospital Wuhan China.

We described a case where peri-left bundle branch pacing (PLBP) may become an alternative approach in difficult His bundle pacing (HBP) following atrioventricular nodal ablation in a patient with atrial fibrillation. After atrioventricular nodal ablation, the HBP lead was removed to another LBB position distal to the first PLBP lead, due to acute threshold increase. At 3 month follow-up, PLBP exhibited acceptable pacing parameters without any adverse event.
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http://dx.doi.org/10.1002/joa3.12299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011818PMC
February 2020

A survey of Chinese consumers' knowledge, beliefs and behavioural intentions regarding salt intake and salt reduction.

Public Health Nutr 2020 06 13;23(8):1450-1459. Epub 2020 Jan 13.

School of Public Health, Physiotherapy and Sports Science, University College Dublin, Belfield, Dublin 4, Republic of Ireland.

Objective: Globally, China is among the 'saltiest' nations. In order to support current nationwide salt reduction initiatives, we investigated Chinese consumers' knowledge, beliefs and behaviours related to salt intake and salt reduction.

Design: A cross-sectional face-to-face survey was carried out, focusing on salt knowledge, beliefs and behaviours related to salt intake and salt reduction, perceptions of salt reduction responsibility and support for different national strategies.

Setting: The survey was carried out in China mainland.

Participants: Consumers (n 2444) from six of seven major geographical regions in China participated in the survey. After data cleaning, a sample of 2430 was included in the final analysis.

Results: A majority of Chinese consumers believed that salt added during home cooking was the biggest contributor to their salt intake. Knowledge gaps existed in the awareness of salt hidden in certain foods and flavouring products. Chinese consumers in general were interested in lowering their salt intake. They were aware of salt reduction tools, but the adoption level was low. Consumers expressed strong support for promotion of salt-restriction spoons and public education, but not fiscal policies (e.g. salt-related tax or subsidies). In terms of individual differences, education status demonstrated a substantial impact on salt reduction knowledge and behaviour.

Conclusions: There is still big room to 'shake' Chinese consumers' salt habit. The present study provides important evidence and consumer insights to support China's efforts to meet its salt reduction targets.
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http://dx.doi.org/10.1017/S1368980019003689DOI Listing
June 2020

Guanine-Stabilized Formamidinium Lead Iodide Perovskites.

Angew Chem Int Ed Engl 2020 Mar 3;59(12):4691-4697. Epub 2020 Feb 3.

Laboratory of Photonics and Interfaces, Institut des Sciences et Ingénierie Chimiques, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.

Formamidinium (FA) lead iodide perovskite materials feature promising photovoltaic performances and superior thermal stabilities. However, conversion of the perovskite α-FAPbI phase to the thermodynamically stable yet photovoltaically inactive δ-FAPbI phase compromises the photovoltaic performance. A strategy is presented to address this challenge by using low-dimensional hybrid perovskite materials comprising guaninium (G) organic spacer layers that act as stabilizers of the three-dimensional α-FAPbI phase. The underlying mode of interaction at the atomic level is unraveled by means of solid-state nuclear magnetic resonance spectroscopy, X-ray crystallography, transmission electron microscopy, molecular dynamics simulations, and DFT calculations. Low-dimensional-phase-containing hybrid FAPbI perovskite solar cells are obtained with improved performance and enhanced long-term stability.
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http://dx.doi.org/10.1002/anie.201912051DOI Listing
March 2020

Amide Additives Induced a Fermi Level Shift To Improve the Performance of Hole-Conductor-Free, Printable Mesoscopic Perovskite Solar Cells.

J Phys Chem Lett 2019 Nov 24;10(21):6865-6872. Epub 2019 Oct 24.

Michael Grätzel Center for Mesoscopic Solar Cells, Wuhan National Laboratory for Optoelectronics, School of Optical and Electronic Information , Huazhong University of Science and Technology , Wuhan 430074 , Hubei , P. R. China.

Solution-processable organic-inorganic perovskite solar cells have attracted much attention in the past few years. Energy level alignment is of great importance for improving the performance of perovskite solar cells because it strongly influences charge separation and recombination. In this report, we introduce three amide additives, namely, formamide, acetamide, and urea, into the MAPbI perovskite by mixing them directly in perovskite precursor solutions. The Fermi level of MAPbI shifts from -4.36 eV to -4.63, -4.65, and -4.61 eV, respectively, upon addition of these additives. The charge transfer between perovskite and mp-TiO is found to be promoted as determined via TRPL spectra, and recombination in the perovskite is suppressed. As a result, the built-in electric field () of the printable, hole-conductor-free mesoscopic perovskite solar cells based on these perovskites with amide additives is enhanced and a peak power conversion efficiency of 15.57% is obtained.
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http://dx.doi.org/10.1021/acs.jpclett.9b02463DOI Listing
November 2019

Precision detection of liver metastasis by collagen-targeted protein MRI contrast agent.

Biomaterials 2019 12 6;224:119478. Epub 2019 Sep 6.

Department of Chemistry, Georgia State University, Atlanta, GA, 30303, USA; Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA, 30303, USA. Electronic address:

The Liver is the most common organ for metastasis for various cancers, including uveal melanoma, the most common primary intraocular tumor. Uveal melanoma metastasizes to the liver in ~90% of patients, and results in death in almost all cases due to late detection and lack of effective treatment. There is a pressing unmet medical need to develop MRI contrast agents and imaging methodologies with desired sensitivity and specificity to overcome the high heterogeneous background and in vivo properties as well as reduced toxicity. Herein, we report the development of a collagen targeting protein contrast agent (ProCA32.collagen1), since collagen is a diagnostic biomarker and therapeutic target for many types of primary and metastatic cancers and the tumor microenvironment. In addition to a strong affinity to collagen I, ProCA32.collagen1 possesses high relaxivities (r and r are 68.0 ± 0.25 and 100.0 ± 0.32 mM s at 1.4 T, respectively, and 42.6 ± 1.0 and 217 ± 2.4 mMs at 7.0 T per particle). ProCA32.collagen1 also has strong serum stability against degradation, resistance to transmetallation, and 10 and 10-fold higher metal selectivity for Gd over Ca and Zn, respectively, compared to clinical contrast agents. ProCA32.collagen1 does not exhibit any cell toxicity for various cell lines. Sensitive detection of liver lesions in animal models can be achieved using multiple imaging methodologies, taking advantage of the dual relaxation property of ProCA32.collagen1. ProCA32.collagen1 enables sensitive and early stage detection of hepatic micrometastasis as small as 0.144 mm and two different tumor growth patterns. Further development of ProCA32.collagen1 has the potential to greatly facilitate non-invasive, early detection and staging of primary and metastatic liver cancers, and devising effective treatments.
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http://dx.doi.org/10.1016/j.biomaterials.2019.119478DOI Listing
December 2019

Differential Regulation of Anthocyanins in Green and Purple Turnips Revealed by Combined De Novo Transcriptome and Metabolome Analysis.

Int J Mol Sci 2019 Sep 6;20(18). Epub 2019 Sep 6.

Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.

Purple turnip ssp. rapa is highly appreciated by consumers but the metabolites and molecular mechanisms underlying the root skin pigmentation remain open to study. Herein, we analyzed the anthocyanin composition in purple turnip (PT) and green turnip (GT) at five developmental stages. A total of 21 anthocyanins were detected and classified into the six major anthocynanin aglycones. Distinctly, PT contains 20 times higher levels of anthocyanins than GT, which explain the difference in the root skin pigmentation. We further sequenced the transcriptomes and analyzed the differentially expressed genes between the two turnips. We found that PT essentially diverts dihydroflavonols to the biosynthesis of anthocyanins over flavonols biosynthesis by strongly down-regulating one flavonol synthase gene, while strikingly up-regulating dihydroflavonol 4-reductase (DFR), anthocyanidin synthase and UDP-glucose: flavonoid-3--glucosyltransferase genes as compared to GT. Moreover, a nonsense mutation identified in the coding sequence of the DFR gene may lead to a nonfunctional protein, adding another hurdle to the accumulation of anthocyanin in GT. We also uncovered several key members of MYB, bHLH and WRKY families as the putative main drivers of transcriptional changes between the two turnips. Overall, this study provides new tools for modifying anthocyanin content and improving turnip nutritional quality.
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http://dx.doi.org/10.3390/ijms20184387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769466PMC
September 2019

Two-Stage Melt Processing of Phase-Pure Selenium for Printable Triple-Mesoscopic Solar Cells.

ACS Appl Mater Interfaces 2019 Sep 5;11(37):33879-33885. Epub 2019 Sep 5.

Michael Grätzel Center for Mesoscopic Solar Cells, Wuhan National Laboratory for Optoelectronics , Huazhong University of Science and Technology , Wuhan 430074 , Hubei , PR China.

Hexagonal selenium with a direct band gap has been developed for optoelectronic applications for more than one century. The major advances in Se solar cells have been made using vacuum or solution-based processing methods. In this work, we demonstrate a new two-stage melt processing (TSMP) method for incorporating Se in printable triple mesoscopic solar cells in the ambient conditions. It is observed that polymerization and depolymerization between several types of selenium chains are simultaneously triggered during the melt processing, from which phase-pure hexagonal selenium is formed in the mesopores of solar cells with high crystallinity. The TSMP method has positive effects on the conduction-band energy level, band gap, and crystal phase of as-deposited Se, as revealed UV electron spectroscopy, UV-vis absorption spectroscopy, and in situ X-ray diffraction. The TSMP-based printable mesoscopic selenium solar cells show a power conversion efficiency of 2%, which is eight times that for devices based on the single-stage melting processing. These findings open up a new research direction of melting processing toward more efficient photovoltaic devices.
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http://dx.doi.org/10.1021/acsami.9b09572DOI Listing
September 2019

Modulation of Acceptor Position in Organic Sensitizers: The Optimization of Intramolecular and Interfacial Charge Transfer Processes.

ACS Appl Mater Interfaces 2019 Aug 29;11(31):27648-27657. Epub 2019 Jul 29.

Sauvage Center for Molecular Sciences, Department of Chemistry , Wuhan University , Wuhan 430072 , China.

With respect to cyanoacryclic acid as the traditional acceptor and anchoring group in dye-sensitized solar cells, the introduction of stronger electron-deficient groups has greatly expanded the scope of molecular design and increased their efficiencies for organic dyes. In this contribution, benzothiadiazole (BTD) was selected as a representative acceptor to illustrate the influence of its position on the photophysical properties and corresponding photovoltaic performances. Through the insertion of BTD in different positions of the framework with the same composition units and orders, four sensitizers were designed and synthesized. The structure-property relationship demonstrated the preference of the suitable position of an electron acceptor for optimization of the spectra response and interfacial charge transfer. In our system, dye with BTD in the middle of the conjugated bridge showed the broadest spectrum and achieved the best photovoltaic performance (8.25%), which may pave a new way to design or optimize the efficient sensitizers by rational design of the acceptor position.
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http://dx.doi.org/10.1021/acsami.9b03428DOI Listing
August 2019

A low-temperature carbon electrode with good perovskite compatibility and high flexibility in carbon based perovskite solar cells.

Chem Commun (Camb) 2019 Feb;55(19):2765-2768

Nano-Science & Technology, College of Physics and Technology, Central China Normal University (CCNU), Wuhan 430079, China.

A low-temperature carbon electrode with good perovskite compatibility is employed in hole-transport-material free perovskite solar cells, and a champion power conversion efficiency (PCE) of 11.7% is obtained. The PCE is enhanced to 14.55% by an interface modification of PEDOT:PSS. The application of this carbon on ITO/PEN substrates is also demonstrated.
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http://dx.doi.org/10.1039/c8cc09905gDOI Listing
February 2019

Organic Dyes based on Tetraaryl-1,4-dihydropyrrolo-[3,2-b]pyrroles for Photovoltaic and Photocatalysis Applications with the Suppressed Electron Recombination.

Chemistry 2018 Dec 8;24(68):18032-18042. Epub 2018 Nov 8.

Department of Chemistry, Wuhan University, Wuhan, 430072, China.

A tetraaryl-1,4-dihydropyrrolo-[3,2-b]pyrroles (TAPP) moiety with the combination of two pyrrole rings and four phenyl moieties demonstrated strong electron-donating ability and nonplanar configuration simultaneously. Once incorporated into the organic dyes as a novel electron donor, it was beneficial for the enhancement of light-harvesting ability and suppression of electron recombination in the photovoltaic and photocatalysis systems. With the linkage of tunable conjugated bridges and electron acceptor, the corresponding organic dyes exhibited improved photovoltaic performance in dye-sensitized solar cells and facilitated photocatalytic hydrogen generation with a highest turnover number (TON) of 4337. Through the detailed investigation of relationship between molecular structures and photovoltaic/photocatalysis property, the connection and difference in molecular design for these two systems are well explained, with the aim to promote the application of dye-sensitized technology in various fields.
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http://dx.doi.org/10.1002/chem.201803688DOI Listing
December 2018

Challenges for commercializing perovskite solar cells.

Science 2018 09;361(6408)

Michael Grätzel Center for Mesoscopic Solar Cells, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China.

Perovskite solar cells (PSCs) have witnessed rapidly rising power conversion efficiencies, together with advances in stability and upscaling. Despite these advances, their limited stability and need to prove upscaling remain crucial hurdles on the path to commercialization. We summarize recent advances toward commercially viable PSCs and discuss challenges that remain. We expound the development of standardized protocols to distinguish intrinsic and extrinsic degradation factors in perovskites. We review accelerated aging tests in both cells and modules and discuss the prediction of lifetimes on the basis of degradation kinetics. Mature photovoltaic solutions, which have demonstrated excellent long-term stability in field applications, offer the perovskite community valuable insights into clearing the hurdles to commercialization.
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http://dx.doi.org/10.1126/science.aat8235DOI Listing
September 2018

Extending lead-free hybrid photovoltaic materials to new structures: thiazolium, aminothiazolium and imidazolium iodobismuthates.

Dalton Trans 2018 May;47(20):7050-7058

School of Chemistry and EaStCHEM, University of Edinburgh, King's Buildings, David Brewster Road, Edinburgh, Scotland EH9 3FJ, UK.

We report on the synthesis, crystal structures, optoelectronic properties and solar cell device studies of three novel organic-inorganic iodobismuthates - [C3H4NS]3[Bi2I9] ([TH]3[Bi2I9]), [C3H4N2]3[Bi2I9] ([IM]3[Bi2I9]) and [C3H5N2S][BiI4] ([AT][BiI4]) as lead-free light harvesters. [TH]3[Bi2I9] and [IM]3[Bi2I9] show zero-dimensional structures, whereas a one-dimensional edge-sharing chain structure of BiI6-octahedra was observed in [AT][BiI4], with interchain short II contacts also giving rise to the possibility of three-dimensional charge transport ability. Accordingly, greater energy dispersion in the band structure of [AT][BiI4] can be observed, and less contribution from the organic moities at the conduction band minimum in [AT][BiI4] than [TH]3[Bi2I9] have been confirmed by density functional theory calculations. Moreover, bandgap values are redshifted from 2.08 eV for [TH]3[Bi2I9] and 2.00 eV for [IM]3[Bi2I9] to 1.78 eV for [AT][BiI4], determined by UV-Vis reflectance spectroscopy. Power conversion efficiency of 0.47% has been achieved by using ([AT][BiI4]) as the light absorber in a hole-conductor-free, fully printable solar cell, with relatively good reproducibility. We also note the observation of a capacitance effect for the first time in a photovoltaic device with bismuth-based solar absorber, which may be related to the mesoporous carbon counter-electrode.
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http://dx.doi.org/10.1039/c8dt00864gDOI Listing
May 2018

Toward Industrial-Scale Production of Perovskite Solar Cells: Screen Printing, Slot-Die Coating, and Emerging Techniques.

J Phys Chem Lett 2018 May 8;9(10):2707-2713. Epub 2018 May 8.

Michael Grätzel Center for Mesoscopic Solar Cells, Wuhan National Laboratory for Optoelectronics, School of Optical and Electronic Information , Huazhong University of Science and Technology , Wuhan 430074 , Hubei , People's Republic of China.

Perovskite solar cells (PSCs) have attracted intensive attention of the researchers and industry due to their high efficiency, low material cost, and simple solution-based fabrication process. Along with the development of device configurations, materials, and fabrication techniques, the efficiency has rapidly increased from the initial 3.8 to recent 22.7%. However, fundamental studies on PSCs are usually yielded through lab-scale procedures and carried out on small-area (≤1 cm) devices. Recently, various deposition methods, such as screen printing, slot-die coating, soft-cover coating, spraying coating, etc., have been developed to enlarge the device area from the millimeters to hundreds of centimeters scale. Herein, we discuss the advances of up-scaling of PSCs and outline the fabrication methods from lab-scale to industrial-scale. Screen printing and slot-die coating have been regarded as the most promising methods toward the mass production of PSCs, and more emerging techniques are also anticipated in this enterprise.
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http://dx.doi.org/10.1021/acs.jpclett.8b00912DOI Listing
May 2018