Publications by authors named "Hongtao Liu"

435 Publications

Atomically sharp interface enabled ultrahigh-speed non-volatile memory devices.

Nat Nanotechnol 2021 May 3. Epub 2021 May 3.

Institute of Physics, Chinese Academy of Sciences, Beijing, People's Republic of China.

The development of high-performance memory devices has played a key role in the innovation of modern electronics. Non-volatile memory devices have manifested high capacity and mechanical reliability as a mainstream technology; however, their performance has been hampered by low extinction ratio and slow operational speed. Despite substantial efforts to improve these characteristics, typical write times of hundreds of micro- or milliseconds remain a few orders of magnitude longer than that of their volatile counterparts. Here we demonstrate non-volatile, floating-gate memory devices based on van der Waals heterostructures with atomically sharp interfaces between different functional elements, achieving ultrahigh-speed programming/erasing operations in the range of nanoseconds with extinction ratio up to 10. This enhanced performance enables new device capabilities such as multi-bit storage, thus opening up applications in the realm of modern nanoelectronics and offering future fabrication guidelines for device scale up.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41565-021-00904-5DOI Listing
May 2021

Continuous Resonance Tuning without Blindness by Applying Nonlinear Properties of PIN Diodes.

Sensors (Basel) 2021 Apr 16;21(8). Epub 2021 Apr 16.

School of Communication and Information Engineering, Shanghai University, Shanghai 200444, China.

Metamaterial antennas consisting of periodical units are suitable for achieving tunable properties by employing active elements to each unit. However, for compact metamaterials with a very limited number of periodical units, resonance blindness exists. In this paper, we introduce a method to achieve continuous tuning without resonance blindness by exploring hence, taking advantage of nonlinear properties of PIN diodes. First, we obtain the equivalent impedance of the PIN diode through measurements, then fit these nonlinear curves with mathematical expressions. Afterwards, we build the PIN diode model with these mathematical equations, making it compatible with implementing co-simulation between the passive electromagnetic model and the active element of PIN diodes and, particularly, the nonlinear effects can be considered. Next, we design a compact two-unit metamaterial antenna as an example to illustrate the electromagnetic co-simulation. Finally, we implement the experiments with a micro-control unit to validate this method. In addition, the nonlinear stability and the supplying voltage tolerance of nonlinear states for both two kinds of PIN diodes are investigated as well. This method of obtaining smooth tuning with nonlinear properties of PIN diodes can be applied to other active devices, if only PIN diodes are utilized.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21082816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073602PMC
April 2021

Novel agents and regimens for hematological malignancies: recent updates from 2020 ASH annual meeting.

J Hematol Oncol 2021 Apr 21;14(1):66. Epub 2021 Apr 21.

Department of Medicine, New York Medical College and Westchester Medical Center, Valhalla, NY, 10595, USA.

Antibodies and chimeric antigen receptor-engineered T cells (CAR-T) are increasingly used for cancer immunotherapy. Small molecule inhibitors targeting cellular oncoproteins and enzymes such as BCR-ABL, JAK2, Bruton tyrosine kinase, FLT3, BCL-2, IDH1, IDH2, are biomarker-driven chemotherapy-free agents approved for several major hematological malignancies. LOXO-305, asciminib, "off-the-shelf" universal CAR-T cells and BCMA-directed immunotherapeutics as well as data from clinical trials on many novel agents and regimens were updated at the 2020 American Society of Hematology (ASH) Annual Meeting. Major developments and updates for the therapy of hematological malignancies were delineated at the recent Winter Symposium and New York Oncology Forum from the Chinese American Hematologist and Oncologist Network (CAHON.org). This study summarized the latest updates on novel agents and regimens for hematological malignancies from the 2020 ASH annual meeting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13045-021-01077-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059303PMC
April 2021

Impact of depth of clinical response on outcomes of acute myeloid leukemia patients in first complete remission who undergo allogeneic hematopoietic cell transplantation.

Bone Marrow Transplant 2021 Apr 16. Epub 2021 Apr 16.

Haematology Research Centre, Department of Immunology and Inflammation, Imperial College London, London, UK.

Acute myeloid leukemia (AML) patients often undergo allogeneic hematopoietic cell transplantation (alloHCT) in first complete remission (CR). We examined the effect of depth of clinical response, including incomplete count recovery (CRi) and/or measurable residual disease (MRD), in patients from the Center for International Blood and Marrow Transplantation Research (CIBMTR) registry. We identified 2492 adult patients (1799 CR and 693 CRi) who underwent alloHCT between January 1, 2007 and December 31, 2015. The primary outcome was overall survival (OS). Multivariable analysis was performed to adjust for patient-, disease-, and transplant-related factors. Baseline characteristics were similar. Patients in CRi compared to those in CR had an increased likelihood of death (HR: 1.27; 95% confidence interval: 1.13-1.43). Compared to CR, CRi was significantly associated with increased non-relapse mortality (NRM), shorter disease-free survival (DFS), and a trend toward increased relapse. Detectable MRD was associated with shorter OS, shorter DFS, higher NRM, and increased relapse compared to absence of MRD. The deleterious effects of CRi and MRD were independent. In this large CIBMTR cohort, survival outcomes differ among AML patients based on depth of CR and presence of MRD at the time of alloHCT. Further studies should focus on optimizing post-alloHCT outcomes for patients with responses less than CR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41409-021-01261-6DOI Listing
April 2021

Toll protein family structure, evolution and response of the whiteleg shrimp (Litopenaeus vannamei) to exogenous iridescent virus.

J Fish Dis 2021 Apr 9. Epub 2021 Apr 9.

Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen, China.

Whiteleg shrimp is a widely cultured crustacean, but frequent disease outbreaks have decreased production and caused significant losses. Toll-like receptors (TLRs) comprise a large innate immune family that is involved in the innate immune response. However, understanding of their regulatory mechanism is limited. In this study, PacBio sequencing and Illumina sequencing were applied to the gill and hepatopancreas tissues of whiteleg shrimp and an integrated transcript gene set was established. The upregulation of Toll1, Toll2 and Toll3 transcripts in the hepatopancreas tissue of whiteleg shrimp after iridescent virus infection implies that these proteins are involved in the immune response to the virus; simultaneously, the TRAF6 and relish transcripts in the Toll pathway were also upregulated, implying that the Toll pathway was activated. We predicted the three-dimensional structure of the five Toll proteins in whiteleg shrimp and humans and constructed a phylogenetic tree of the Toll protein family. In addition, there was a large discrepancy of Toll1 between invertebrates and vertebrates, presumably because of the loss of Toll1 protein sequence during the evolution process from invertebrates to vertebrates. Our research will improve the cognition of Toll protein family in invertebrates in terms of evolution, structure and function and provide theoretical guidance for researchers in this field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jfd.13374DOI Listing
April 2021

Characterization of the complete mitochondrial genome of convex reef crab (Forskål, 1775).

Mitochondrial DNA B Resour 2021 Mar 23;6(3):1147-1149. Epub 2021 Mar 23.

Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, PR China.

The complete mitochondrial genome of convex reef crab was determined and characterized for the first time from the South China Sea. The whole mitogenome is 15,766 bp long and consists of 22 tRNA genes, 2 rRNA genes, 13 protein-coding genes (PCGs), and 1 control region. The nucleotide composition of the mitogenome is significantly biased (A, G, T, and C is 36.91%, 17.94%, 34.95%, and 10.19%, respectively) with A + T contents of 71.86%. All PCGs start with a normal initiation codon ATN and terminate with a standard stop codon except ND1 gene end with TTG. Five microsatellites are identified in mitogenome sequences. The phylogenetic tree showed that was first clustered with , and strongly supports that the recognition of the Carpiliidae as a monophyletic family.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1901618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995858PMC
March 2021

The complete mitochondrial genome of spiny spooner (Herbst, 1785) using high-throughput sequencing.

Mitochondrial DNA B Resour 2021 Mar 18;6(3):985-987. Epub 2021 Mar 18.

Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, China.

The whole mitochondrial genome of the spiny spooner collected from the South China Sea was determined for the first time using high-throughput sequencing. The circular mitogenome of is 15,884 bp, with 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes. The base composition is significantly biased (A, G, T, and C was 37.9%, 17.8%, 34.0%, and 10.3%, respectively) with A + T contents of 71.9%. Among 13 PCGs, all PCGs use a normal ATN as the start codon, and ten of them end with TAA or TAG except COX1, COX2 and CYTB gene using a single T as the stop codon. The phylogenetic tree showed that forms a cluster while form a sister group to this cluster.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1891982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995879PMC
March 2021

The Prognostic Value of Plasma Cell-Free DNA Concentration in the Prostate Cancer: A Systematic Review and Meta-Analysis.

Front Oncol 2021 11;11:599602. Epub 2021 Mar 11.

Department of Molecular Diagnosis, Northern Jiangsu Hospital, Yangzhou University Clinical College, Yangzhou, China.

Objective: By virtue of largely disparate clinical outcomes of prostate cancer (PCA), there is a pressing need to search for useful biomarkers for PCA prognosis. Cell-free DNA (cfDNA) is a promising biomarker for detecting, monitoring, and predicting survival of prostate cancer (PCA). However, the utility of total cfDNA quantitation in PCA in clinical setting remains elusive. Here, we performed a thorough meta-analysis to assess the prognostic value of cfDNA concentration for patients with PCA. In addition, we tested the possibility of the combination of PSA and cfDNA test results to improve the prediction power in PCA prognosis.

Method And Materials: More than six databases, including PubMed, Web of Science, Medline, PMC, EMBASE and the Cochrane Library were searched. Results yielded all eligible articles from the date of inception to June 30, 2020. Continuous, diagnostic, and prognostic variables in cfDNA in PCA were included in the meta-analysis by STATA.

Results: A total of 23 articles were enrolled in our meta-analysis: 69.6% (16/23) were related to diagnosis, and 56.5% (13/23) were related to prognosis. The pooled concentration of cfDNA in PCA patients was significantly higher than in the control group (SMD = 0.89, 95%CI = 0.53, 1.26), mirroring results for the prostate-specific antigen (PSA). For the detection test variables, the SROC with 95%CI was 0.87 (0.84-0.90) for cfDNA concentration. In terms of prognostic variables, the concentrations of cfDNA were significantly related with progression-free survival (PFS, logHR = 0.84 (95%CI0.39, 1.28) and overall survival [OS, log HR = 0.60 (95%CI0.29, 0.90)]. Lastly, the test showed no significant publication bias in the present meta-analysis, excluding the diagnostic meta-analysis.

Conclusions: The concentration of cell-free DNA is high in the prostate cancer patients. The present study substantiates the prognostic value of the cfDNA concentration. High concentration cfDNA correlates with poor disease outcome of CRPC. The study cohort with large sample size is needed to evaluate the prognosis value of cfDNA in the future. We also emphasized that combination of PSA and cf DNA quantitation is important in future large individual meta study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.599602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991303PMC
March 2021

The complete mitochondrial genome of longlegged spiny lobster (A. Milne Edwards, 1868).

Mitochondrial DNA B Resour 2021 Mar 1;6(2):660-662. Epub 2021 Mar 1.

Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, China.

In this study, we first determined and characterized the complete mitochondrial genome of longlegged spiny lobster from South China Sea. The mitogenome is 15,739 bp long, and consists of 22 tRNA genes, two rRNA genes, 13 protein-coding genes (PCGs), and one control region. The nucleotide composition of mitogenome is significantly biased (A, G, T, and C was 32.06%, 14.36%, 32.42%, and 21.16%, respectively) with A + T contents of 64.48%. Among 13 PCGs, COX1 gene used an unusual initiation codon CAA, COX1, COX2, ND4 and CYTB genes were ended with an incomplete stop codon T, and ND5 gene with an abnormal stop codon ATT. One microsatellite (C) was identified in mitogenome located in the control region. Phylogenetic tree showed that was first clustered with , then together with and .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1878952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928049PMC
March 2021

Van Der Waals Epitaxial Growth and Phase Transition of Layered FeSe Nanocrystals.

Adv Mater 2021 Apr 23;33(17):e2008456. Epub 2021 Mar 23.

College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518052, P. R. China.

Layered iron chalcogenides (FeX, X = S, Se, Te) provide excellent platforms to study intertwined phase transitions, superconductivity, and magnetism. However, layered iron dichalcogenides (FeX , X = S, Se, Te) are rarely reported and their intrinsic properties are still unknown. Here, phase-pure layered iron diselenide (FeSe ) nanocrystals are epitaxially grown on mica by the sublimed-salt-assisted chemical vapor deposition method at atmospheric pressure. The layered atomic structure of FeSe is confirmed by X-ray diffraction and atomic-resolution scanning transmission electron microscopy. Electrical transport shows that the layered FeSe is a metal with high conductivity and a phase transition at ≈11 K. The phase transition manifests itself as a kink in the temperature-dependent resistivity, as well as anomalous magnetoresistance (MR) appearing around the phase-transition temperature. The MR changes from negative to positive, accompanied by large hysteresis near the phase-transition temperature upon cooling. The negative MR and hysteresis might originate from magnetic field suppression scattering of spin fluctuations and competition of magnetic interactions induced by the phase transition, respectively. Layered iron dichalcogenide will be potential candidate to explore novel quantum phenomena and other applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202008456DOI Listing
April 2021

Emerging agents and regimens for AML.

Authors:
Hongtao Liu

J Hematol Oncol 2021 Mar 23;14(1):49. Epub 2021 Mar 23.

Section of Hematology/Oncology, Department of Medicine, The University of Chicago Medical Center, 5841 S. Maryland Ave, MC 2115, Chicago, IL, 60637-1470, USA.

Until recently, acute myeloid leukemia (AML) patients used to have limited treatment options, depending solely on cytarabine + anthracycline (7 + 3) intensive chemotherapy and hypomethylating agents. Allogeneic stem cell transplantation (Allo-SCT) played an important role to improve the survival of eligible AML patients in the past several decades. The exploration of the genomic and molecular landscape of AML, identification of mutations associated with the pathogenesis of AML, and the understanding of the mechanisms of resistance to treatment from excellent translational research helped to expand the treatment options of AML quickly in the past few years, resulting in noteworthy breakthroughs and FDA approvals of new therapeutic treatments in AML patients. Targeted therapies and combinations of different classes of therapeutic agents to overcome treatment resistance further expanded the treatment options and improved survival. Immunotherapy, including antibody-based treatment, inhibition of immune negative regulators, and possible CAR T cells might further expand the therapeutic armamentarium for AML. This review is intended to summarize the recent developments in the treatment of AML.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13045-021-01062-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989091PMC
March 2021

One-dimensional weak antilocalization effect in 1T'-MoTenanowires grown by chemical vapor deposition.

J Phys Condens Matter 2021 Apr 23;33(18). Epub 2021 Apr 23.

Institute of Physics, Chinese Academy of Sciences, PO Box 603, Beijing 100190, People's Republic of China.

We present a chemical vapor deposition method for the synthesizing of single-crystal 1T'-MoTenanowires and the observation of one-dimensional weak antilocalization effect in 1T'-MoTenanowires for the first time. The diameters of the 1T'-MoTenanowires can be controlled by changing the flux of H/Ar carrier gas. Spherical-aberration-corrected transmission electron microscopy, selected area electron diffraction and energy dispersive x-ray spectroscopy (EDS) reveal the 1T' phase and the atomic ratio of Te/Mo closing to 2:1. The resistivity of 1T'-MoTenanowires shows metallic behavior and agrees well with the Fermi liquid theory (<20 K). The coherence length extracted from 1D Hikami-Larkin-Nagaoka model with the presence of strong spin-orbit coupling is proportional to, indicating a Nyquist electron-electron interaction dephasing mechanism at one dimension. These results provide a feasible way to prepare one-dimensional topological materials and is promising for fundamental study of the transport properties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-648X/abef99DOI Listing
April 2021

High expression of CD52 in adipocytes: a potential therapeutic target for obesity with type 2 diabetes.

Aging (Albany NY) 2021 Mar 11;13(8):11043-11060. Epub 2021 Mar 11.

The Center of Gastrointestinal and Minimally Invasive Surgery, The Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu 610031, China.

The aim of the present study was to evaluate the involvement of CD52 in adipocytes as well as to explore its effect on type 2 diabetes mellitus (T2DM), and to improve our understanding of the potential molecular events of obesity with type 2 diabetes. Global changes in the CD52 expression patterns were detected in adipocytes and preadipocytes derived from obese and lean individuals. In particular, CD52 was identified as significantly differentially upregulated and was analyzed, both and , using various approaches. experiments, CD52 was a significantly up-regulated mRNA in mature adipocytes and preadipocytes. In addition, CD52 gradually increased with the differentiation of preadipocytes. experiments, the expression of CD52 in high-fat diet (HFD) -fed mice tended to be higher than that in regular diet (RD) -fed mice. Further analysis showed that CD52 expression was positively correlated with Smad3 and TGF-β in mice, and the downregulation of CD52 was accompanied by increased glucose tolerance and insulin sensitivity. Moreover, a comparison of CD4+CD52 T cells and CD4+CD52 T cells showed that many T2DM-related genes were aberrantly expressed. Overall, CD52 may functioned as an important potential target for obesity with T2DM via TGF-β/Smad3 axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.202714DOI Listing
March 2021

Colistin-resistance mcr genes in Klebsiella pneumoniae from companion animals.

J Glob Antimicrob Resist 2021 Mar 1;25:35-36. Epub 2021 Mar 1.

Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, 130062, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jgar.2021.02.023DOI Listing
March 2021

Post-Transplant Cyclophosphamide (PTCy) is Associated with Increased Cytomegalovirus Infection: A CIBMTR Analysis.

Blood 2021 Mar 3. Epub 2021 Mar 3.

CIBMTR, United States.

Prior studies suggest increased CMV infection following haploidentical donor transplantation with post-transplant cyclophosphamide (HaploCy). The role of allograft source and PTCy in CMV infection and disease is unclear. We analyzed the effect of graft source and PTCy on incidence of CMV infection as well as transplant outcomes as it relates to CMV serostatus and occurrence of CMV infection by d180. We examined patients reported to CIBMTR between 2012-2017 who had received HaploCy (n = 757), Sib with PTCy (SibCy, n=403), or Sib with calcineurin inhibitor-based prophylaxis (SibCNI, n=1605) for AML/ALL/MDS. Cumulative incidences of CMV infection by d180 were 42% (99% CI, 37-46), 37% (31 - 43), and 23% (20 - 26), respectively [p<0.001]. CMV end-organ disease was statistically comparable. CMV infection risk was highest for CMV-Seropositive recipients (R+), but significantly higher in PTCy recipients regardless of donor [HaploCy (n=545): HR 50.3 (14.4 - 175.2); SibCy (n=279): HR 47.7 (13.3 - 171.4); SibCNI (n=1065): HR 24.4 (7.2 - 83.1); p<0.001]. D+/R- patients also had increased risk for CMV infection. Among seropositive recipients or those developing CMV infection, HaploCy had worse OS and NRM. Relapse was unaffected by CMV infection or serostatus. PTCy was associated with lower chronic GVHD overall, but CMV infection in PTCy recipients was associated with higher cGVHD (p=0.006). PTCy, regardless of donor, is associated with higher incidence of CMV infection, augmenting the risk of seropositivity. Additionally CMV infection may negate the cGVHD protection of PTCy. This study supports aggressive prevention strategies in all patients receiving PTCy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1182/blood.2020009362DOI Listing
March 2021

Semi-In-Vivo Pull-Down Assay for Blue Light-Dependent Protein Interactions.

Methods Mol Biol 2021 ;2297:161-166

National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences, Shanghai, P. R. China.

Cryptochromes are photolyase-like blue-light receptors found in all major evolutionary lineages (Ahmad and Cashmore, Nature 366:162-166, 1993; Lin, Plant Physiol 110:1047, 1996; Cashmore, Cell 114:537-543, 2003; Partch and Sancar, Methods Enzymol 393:726-745, 2005). Arabidopsis cryptochrome 1 (CRY1) and cryptochrome 2 (CRY2) mediate primarily blue-light inhibition of hypocotyl elongation and photoperiodic control of floral initiation (Ahmad and Cashmore, Nature 366:162-166, 1993; Somers et al., Science, 282:1488-1490, 1998; Guo et al., Science 279 (5355):1360-1363, 1998; Yu et al., Arabidopsis Book 8:e0135, 2010). It has been proposed that phototransduction of cryptochromes involves the blue-light-dependent protein interactions, such as AtCRY2-CIB1 (CRYPTOCHROME-INTERACTING BASIC-HELIX-LOOP-HELIX 1), AtCRY1-PIF4 (PHYTOCHROME INTERACTING FACTOR 4) modules, sequentially mediate gene expression and plant growth (Liu et al., Science 322 (5907):1535-1539, 2008; Ma et al., Proc Natl Acad Sci U S A 113 (1):224-229, 2016; Wang et al., Science 354:343-347, 2016). Cryptochromes also showed blue light response in vitro when expressed in Sf9 insect cells using the baculovirus expression system, thus the wavelength-specific CRY2-CIB1 interaction can also be observed in Semi-in-vivo pull-down assay (Li et al., Proc Natl Acad Sci U S A 108 (51):20844-20849, 2011; Liu et al., EMBO Reports, 2018). Here, we describe the detailed process of blue light-dependent CRY2-CIB1 interaction in Semi-in-vivo conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-0716-1370-2_17DOI Listing
January 2021

Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System.

Methods Mol Biol 2021 ;2297:155-160

National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences, Shanghai, P. R. China.

The Bac-to-Bac Baculovirus Expression System provides a rapid and efficient method to generate recombinant cryptochrome (CRY) proteins with chromophore flavin (FAD), which showed blue light response in vitro.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-0716-1370-2_16DOI Listing
January 2021

Streptococcus suis serotype 2 enolase interaction with host brain microvascular endothelial cells and RPSA-induced apoptosis lead to loss of BBB integrity.

Vet Res 2021 Feb 22;52(1):30. Epub 2021 Feb 22.

Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun, Jilin, 130062, People's Republic of China.

Host proteins interacting with pathogens are receiving more attention as potential therapeutic targets in molecular medicine. Streptococcus suis serotype 2 (SS2) is an important cause of meningitis in both humans and pigs worldwide. SS2 Enolase (Eno) has previously been identified as a virulence factor with a role in altering blood brain barrier (BBB) integrity, but the host cell membrane receptor of Eno and The mechanism(s) involved are unclear. This study identified that SS2 Eno binds to 40S ribosomal protein SA (RPSA) on the surface of porcine brain microvascular endothelial cells leading to activation of intracellular p38/ERK-eIF4E signalling, which promotes intracellular expression of HSPD1 (heat-shock protein family D member 1), and initiation of host-cell apoptosis, and increased BBB permeability facilitating bacterial invasion. This study reveals novel functions for the host-interactional molecules RPSA and HSPD1 in BBB integrity, and provides insight for new therapeutic strategies in meningitis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13567-020-00887-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898445PMC
February 2021

Effect of inorganic additives (rock phosphate, PR and boron waste, BW) on the passivation of Cu, Zn during pig manure composting.

J Environ Manage 2021 May 17;285:112101. Epub 2021 Feb 17.

Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China. Electronic address:

The bioavailability of heavy metals in compost is critical for their agronomic value. The effect of inorganic additives (rock phosphate, PR and boron waste, BW) on Copper (Cu) and Zinc (Zn) bioavailability during co-compost of swine manure and rice straw was assessed using sequential extraction procedure (European Community Bureau of Reference). The result showed that both additives, applied at rates of 2.5%-7.5% (w/w) could promote the change of exchangeable Cu and reducible Cu into oxidizable Cu, thereby reducing their bioavailability factor (BF) by 15.5%-47.2%. While additives provided no significant reduction in BF of Zn, the shift from exchangeable Zn into reducible Zn can still reduce the mobility of Zn. Based on redundancy analysis (RDA), organic matter (OM) and electrical conductivity (EC) were identified as the most important controlling factors for redistribution of Cu and Zn fractions during composting. The inorganic additives strengthened the passivation of Cu and Zn bioavailability by stimulating OM degradation. The 7.5% (w/w) rock phosphate showed best passivating effect on the bioavailability of Cu.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2021.112101DOI Listing
May 2021

Dipole-bound and valence excited states of AuF anions via resonant photoelectron spectroscopy.

J Chem Phys 2021 Feb;154(7):074303

Department of Physics, State Key Laboratory of Low Dimensional Quantum Physics, Tsinghua University, Beijing 10084, China.

Gold fluoride is a very unique species. In this work, we reported the resonant photodetachment spectra of cryogenically cooled AuF via the slow-electron velocity-map imaging method. We determined the electron affinity of AuF to be 17 976(8) cm or 2.2287(10) eV. We observed a dipole-bound state with a binding energy of 24(8) cm, a valence excited state with a binding energy of 1222(11) cm, and a resonant state with an energy of 814(12) cm above the photodetachment threshold. An unusual vibrational transition with Δn = -3 was observed in the autodetachment from the dipole-bound state. Moreover, two excited states of neutral AuF were recognized for the first time, located at 13 720(78) cm and 16 188(44) cm above the AuF ground state.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1063/5.0038560DOI Listing
February 2021

Development of a Novel Highly Spontaneous Metastatic Model of Esophageal Squamous Cell Carcinoma Using Renal Capsule Technology.

Onco Targets Ther 2021 4;14:785-793. Epub 2021 Feb 4.

Department of Pharmacology, School of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China.

Purpose: Increasing evidence has demonstrated that animal models are imperative to investigate the potential molecular mechanism of metastasis and discover anti-metastasis drugs; however, efficient animal models to unveil the underlying mechanisms of metastasis in esophageal squamous cell carcinoma (ESCC) are limited.

Methods: ESCC cell EC9706 with high invasiveness was screened by repeated Transwell assays. Its biological characteristics were identified by flow cytometry as well as by the wound healing and CCK-8 assays. Besides, the levels of epithelial-mesenchymal transition-related markers were examined using Western blotting. Parental (EC9706-I) and subpopulation (EC9706-I) cells were employed to establish the renal capsule model. Next, the tumor growth was detected by a live animal imaging system, and hematoxylin and eosin staining was applied to evaluate the metastatic status in ESCC.

Results: EC9706-I cells showed rapid proliferation ability, S phase abundance, and high invasive ability; obvious upregulation in N-cadherin, Snail, Vimentin, and Bit1; and downregulation in E-cadherin. EC9706-I cells were less sensitive to the chemotherapy drug 5-fluorouracil than EC9706-I cells; however, both cell lines reached a tumorigenesis rate of 100% in the renal capsule model. The live animal imaging system revealed that the tumors derived from EC9706-I cells grew more slowly than those from EC9706-I cells at weeks 3-14. The EC9706-I xenograft model displayed a spontaneous metastatic site, including kidney, heart, liver, lung, pancreas, and spleen, with a distant metastatic rate of 80%.

Conclusion: Our data suggested that the metastatic model was successfully established, providing a novel platform for further exploring the molecular mechanisms of metastasis in ESCC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S290564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872218PMC
February 2021

The complete mitochondrial genome of pronghorn spiny lobster (Olivier, 1791).

Mitochondrial DNA B Resour 2021 Jan 17;6(1):148-150. Epub 2021 Jan 17.

Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, China.

In this paper, we determined and characterized the complete mitochondrial genome of Pronghorn spiny lobster for the first time from South China Sea. The mitogenome is 15,671 bp long, and consists of 22 tRNA genes, 2 rRNA genes, 13 protein-coding genes (PCGs), and 1 control region. The nucleotide composition of mitogenome is significantly biased (A, G, T, and C was 33.62, 13.32, 32.31, and 20.75%, respectively) with A + T contents of 65.93%. Almost PCGs used a standard initiation codon or stop codon, except COX2, ND3, ND4 and ND1 were terminated with an incomplete stop codon T and ND5 ended with TA. One microsatellite (C) was identified in the control region of mitogenome sequences. Phylogenetic tree showed that was first clustered with and .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2020.1852899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832592PMC
January 2021

Bloodless chimeric antigen receptor (CAR) T-cell therapy in Jehovah's Witnesses.

Leuk Lymphoma 2021 Feb 3:1-7. Epub 2021 Feb 3.

Department of Medicine, Section of Hematology/Oncology, University of Chicago, Chicago, IL, USA.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10428194.2021.1876868DOI Listing
February 2021

Modulation of phagosome phosphoinositide dynamics by a Legionella phosphoinositide 3-kinase.

EMBO Rep 2021 Mar 25;22(3):e51163. Epub 2021 Jan 25.

Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

The phagosome harboring the bacterial pathogen Legionella pneumophila is known to be enriched with phosphatidylinositol 4-phosphate (PtdIns4P), which is important for anchoring a subset of its virulence factors and potentially for signaling events implicated in the biogenesis of the Legionella-containing vacuole (LCV) that supports intracellular bacterial growth. Here we demonstrate that the effector MavQ is a phosphoinositide 3-kinase that specifically catalyzes the conversion of phosphatidylinositol (PtdIns) into PtdIns3P. The product of MavQ is subsequently phosphorylated by the effector LepB to yield PtdIns(3,4)P2, whose 3-phosphate is then removed by another effector SidF to generate PtdIns4P. We also show that MavQ is associated with the LCV and the ∆mavQ mutant displays phenotypes in the anchoring of a PtdIns4P-binding effector similar to those of ∆lepB or ∆sidF mutants. Our results establish a mechanism of de novo PtdIns4P biosynthesis by L. pneumophila via a catalysis axis comprised of MavQ, LepB, and SidF on the surface of its phagosome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.15252/embr.202051163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926237PMC
March 2021

Identification of -benzothiazolyl-2-benzenesulfonamides as novel ABCA1 expression upregulators.

RSC Med Chem 2020 Mar 11;11(3):411-418. Epub 2020 Mar 11.

Hebei Key Laboratory of Organic Functional Molecules , College of Chemistry and Materials Science , Hebei Normal University , Shijiazhuang , 050024 , China . Email:

ATP binding cassette transporter A1 (ABCA1) is a critical transporter that mediates cellular cholesterol efflux from macrophages to apolipoprotein A-I (ApoA-I). Therefore, increasing the expression level of ABCA1 is anti-atherogenic and ABCA1 expression upregulators have become novel choices for atherosclerosis treatment. In this study, a series of -benzothiazolyl-2-benzenesulfonamides, based on the structure of WY06 discovered in our laboratory, were designed and synthesized as novel ABCA1 expression upregulators. Based on an ABCA1 upregulatory cell model, ABCA1 upregulation of target compounds was evaluated. Compounds , , and have good upregulated ABCA1 expression activities, with EC values of 0.97, 0.37, and 0.41 μM, respectively. A preliminary structure-activity relationship is summarized. Replacing the methoxy group on the benzothiazole moiety of WY06 with a fluorine or chlorine atom and exchanging the ester group with a cyano group resulted in more potent ABCA1 upregulating activity. Moreover, compound increased ABCA1 mRNA and protein expression and significantly promoted cholesterol efflux in RAW264.7 cells. In conclusion, -benzothiazolyl-2-benzenesulfonamides were identified as novel ABCA1 expression upregulators.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9md00556kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593777PMC
March 2020

The complete mitochondrial genome of Aesop slipper lobster (De Haan, 1841).

Mitochondrial DNA B Resour 2020 Sep 22;5(3):3404-3405. Epub 2020 Sep 22.

Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, China.

The complete mitochondrial genome of from South China Sea was first determined and characterized. With a length of 15,666 bp, the circular mitogenome of consists of 22 tRNA genes, two rRNA genes, 13 protein-coding genes (PCGs), and one control region. The nucleotide composition is significantly biased (A, G, T, and C was 32.05%, 12.45%, 33.97%, and 21.53%, respectively) with A + T contents of 66.02%. Two PCGs used an unusual initiation codon, and five PCGs were ended with an uncomplete or abnormal stop codon. One microsatellite was identified in the mitogenome located in ND4 gene. Phylogenetic analysis demonstrated that was first clustered with , then together with S.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2020.1823274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782161PMC
September 2020

Caveolae/rafts protect human cerebral microvascular endothelial cells from Streptococcus suis serotype 2 α-enolase-mediated injury.

Vet Microbiol 2021 Mar 6;254:108981. Epub 2021 Jan 6.

College of Veterinary Medicine, Jilin University, Changchun, PR China; College of Animal Science, Yangtze University, Jingzhou, Hubei, 434023, PR China. Electronic address:

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that causes meningitis. The ubiquitously expressed 40S ribosome protein SA (RPSA) is a multifunctional protein involved in the pathogenesis of multiple pathogens, especially those causing meningitis. However, the role of RPSA in SS2-induced meningitis is not clear. In this study, immunofluorescence staining revealed that SS2 infection promoted the intracellular transfer of RPSA to the surface of human cerebral microvascular endothelial cells (HCMECs). Moreover, SS2 infection promoted the accumulation of caveolin 1 (CAV1) and the formation of membrane bulges where RPSA enveloped CAV1 on the cell surface. SS2 infection also caused dynamic changes in the localization of RPSA and CAV1 on the cell surface which could be eliminated by disruption of caveolae/rafts by addition of methyl-β-cyclodextrin (MβCD). Co-immunoprecipitation analysis demonstrated that α-enolase (ENO), a key virulence factor of SS2, interacted with RPSA, and promoted the interaction between RPSA and CAV1. Immunofluorescence staining, western blotting and flow cytometry analyses showed that damaged caveolae/rafts significantly enhanced ENO adhesion to HCMECs, promoted the "destruction" of RPSA by ENO, and enhanced the toxic effect of ENO on HCMECs. Importantly, these effects could be relieved upon the addition of cholesterol. We conclude that caveolae/rafts weaken the toxic effect of SS2 ENO on RPSA-mediated events in HCMECs. Our study has led to better understanding of the roles of RPSA and caveolae/rafts upon SS2 infection, and a new pathological role for RPSA in infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vetmic.2021.108981DOI Listing
March 2021

lncRNA PART1, manipulated by transcriptional factor FOXP2, suppresses proliferation and invasion in ESCC by regulating the miR‑18a‑5p/SOX6 signaling axis.

Oncol Rep 2021 Mar 11;45(3):1118-1132. Epub 2021 Jan 11.

Department of Medical Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, P.R. China.

An increasing number of studies have demonstrated that long non‑coding (lnc)RNAs are associated with tumor invasion, metastasis and the prognosis of patients with a variety of different tumors. However, the roles of lncRNA prostate androgen regulated transcript 1 (PART1) in esophageal squamous cell carcinoma (ESCC) remain unknown. In the present study, reverse transcription‑quantitative PCR was performed to investigate the levels of PART1, SRY‑box transcription factor 6 (SOX6) and miR‑18a‑5p in ESCC tissues and cells. The functions of PART1 in ESCC were demonstrated using Cell Counting Kit‑8 and Matrigel assays. Promoter activity and dual‑luciferase reporter assays, RNA immunoprecipitation and western blot analyses were also used to determine the potential mechanisms of PART1 in ESCC cell lines. It was found that PART1 and SOX6 were both downregulated in ESCC tissues and cells, and their low expression levels were associated with TNM stage, lymph node metastasis and poor prognosis in patients with ESCC. Forkhead box protein P2 (FOXP2) exhibited low expression level in ESCC tissues, and its expression was positively correlated with PART1 expression level in ESCC tissues. FOXP2 was found to bind to the promoter region of PART1 to regulate its expression in ESCC cells. Functionally, PART1 overexpression suppressed cell proliferation and invasion, whereas PART1 downregulation promoted cell proliferation and invasion in the ESCC cell lines. Mechanistically, PART1 functions as a competing endogenous (ce)RNA by sponging miR‑18a‑5p, resulting in the upregulation of the downstream target gene, SOX6, coupled with the inactivation of the β‑catenin/c‑myc signaling axis, to suppress ESCC cell proliferation and invasion. In conclusion, data from the present study unveil a potential ceRNA regulatory pathway, in which PART1 affects SOX6 expression level by sponging miR‑18a‑5p, to ultimately suppress ESCC development and progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/or.2021.7931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859983PMC
March 2021

[Bile acid detection by biosensors-a review].

Sheng Wu Gong Cheng Xue Bao 2020 Dec;36(12):2779-2790

School of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, Hubei, China.

Bile acids facilitate the absorption of lipids, and affect the development of various diseases by regulating intestinal flora structure and modulating immunity and metabolism. It is therefore important to quantitatively detect bile acids. Current analytical methods are still immature due to constituent complexity, structural heterogeneity and bioactive variability of bile acids. Detection of individual bile acids is of significance for pharmacological research, clinical diagnosis and disease prevention. Advances have been made in bile acid analysis from multiple sources including serum, bile, urine and feces, although several limitations still exist for bile acid quantification. Here we review research progress in conventional bile acid assays, including spectrophotometry, thin-layer chromatography, liquid/gas chromatography and liquid/gas chromatography-mass spectrometry. Moreover, we emphasize the development of bile acid biosensors that may have promising prospects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13345/j.cjb.200196DOI Listing
December 2020

Long Non-Coding RNAs: The Regulatory Mechanisms, Research Strategies, and Future Directions in Cancers.

Front Oncol 2020 18;10:598817. Epub 2020 Dec 18.

Department of Pharmacology, School of Basic Medicine, Zhengzhou University, Zhengzhou, China.

The development and application of whole genome sequencing technology has greatly broadened our horizons on the capabilities of long non-coding RNAs (lncRNAs). LncRNAs are more than 200 nucleotides in length and lack protein-coding potential. Increasing evidence indicates that lncRNAs exert an irreplaceable role in tumor initiation, progression, as well as metastasis, and are novel molecular biomarkers for diagnosis and prognosis of cancer patients. Furthermore, lncRNAs and the pathways they influence might represent promising therapeutic targets for a number of tumors. Here, we discuss the recent advances in understanding of the specific regulatory mechanisms of lncRNAs. We focused on the signal, decoy, guide, and scaffold functions of lncRNAs at the epigenetic, transcription, and post-transcription levels in cancer cells. Additionally, we summarize the research strategies used to investigate the roles of lncRNAs in tumors, including lncRNAs screening, lncRNAs characteristic analyses, functional studies, and molecular mechanisms of lncRNAs. This review will provide a short but comprehensive description of the lncRNA functions in tumor development and progression, thus accelerating the clinical implementation of lncRNAs as tumor biomarkers and therapeutic targets.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2020.598817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775490PMC
December 2020