Publications by authors named "Hongmei Guo"

39 Publications

Supercapacitive brophene-graphene aerogel as elastic-electrochemical dielectric layer for sensitive pressure sensors.

J Colloid Interface Sci 2021 May 25;601:355-364. Epub 2021 May 25.

Science and Technology Institute, Hubei Key Laboratory of Advanced Textile Materials & Application, Laboratory for Electron Microscopy, Wuhan Textile University, Wuhan 430073, Hubei, People's Republic of China.

A sensitive pressure sensor based on ultralight and superelastic supercapacitive borophene-graphene aerogel as dielectric layer is reported. The borophene-graphene aerogel not only combines large specific surface area of reduced graphene oxide and high conductivity of borophene, but also exhibits rich porous structure. The strong synergy and intercalation between two different two-dimensional materials benefit electron transfer and electrolyte ion diffusion. On the one hand, the aerogel exhibits greater mass specific capacitance of 330 F g than pure graphene aerogel. More importantly, serving as dielectric layer for pressure sensors with a symmetrical structure, the sensor represents ultra-high sensitivity (0.90 KPa) in the pressure range (<3 KPa), ultra-rapid response time (~110 ms), ultra-low detection limit as 8.7 Pa and excellent working stability after 1000 cycles. In practical application, the sensor demonstrates great performance in monitoring human physiological signals, and agricultural applications.
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http://dx.doi.org/10.1016/j.jcis.2021.05.116DOI Listing
May 2021

Retrospective analysis of pediatric patients with multiple rare-earth magnets ingestion: a single-center experience from China.

BMC Pediatr 2021 04 17;21(1):179. Epub 2021 Apr 17.

Department of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China.

Background: The aim of this study was to characterize patients who ingested multiple rare-earth magnets, reveal the harm of rare-earth magnet foreign bodies in the digestive tract, and develop a clinical management algorithm.

Methods: This was a retrospective review of patients with rare-earth magnet foreign bodies in the digestive tract admitted to a university-affiliated pediatric medical center in China, between January 2016 and December 2019; the subset of medical data evaluated included clinical symptoms, signs, treatments and outcomes.

Results: A total of 51 cases were included in this study, including 36(70.6%) males and 15(29.4%) females. The magnets were passed naturally in 24(47.1%) patients and removed by intervention in 27(52.9%) patients, including 5(9.8%) cases by endoscopy and 22(43.1%) cases by surgery. Twenty-two (43.1%)cases had gastrointestinal obstruction, perforation, and fistula. Compared with the non-surgical group, the time of the surgical group from ingestion to arriving at the hospital was longer([80(5-336) vs 26(2-216)]hours, p < 0.001) while there was no significant difference in the mean age or the number of magnets swallowed.

Conclusions: Magnets are attractive to children, but lead to catastrophic consequences including gastrointestinal obstruction, perforation, and surgical interventions when ingested multiple magnets. Endoscopic resection should be urgently performed in the presence of multiple magnets as early as possible within 24 h, even in asymptomatic patients.
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http://dx.doi.org/10.1186/s12887-021-02642-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052718PMC
April 2021

Alveolar ridge preservation with fibroblast growth factor-2 modified acellular dermal matrix membrane and a bovine-derived xenograft: An experimental in vivo study.

Clin Oral Implants Res 2021 Mar 23. Epub 2021 Mar 23.

Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.

Objective: To investigate the effect of a bone substitute material combined with fibroblast growth factor-2 (FGF-2) loaded barrier membrane on the preservation of alveolar ridge after tooth extraction.

Material And Methods: Four dogs were included. Six extraction sockets of each animal received the 3 treatments and were randomly divided into three groups. Group A: negative control; Group B: bovine xenografts + membrane; and Group C: bovine xenografts + FGF-2-loaded membrane. CBCT and histological analysis were performed to evaluate changes in the width and height of alveolar ridges and extraction socket bone healing 8 weeks post-extraction.

Results: CBCT showed that the alveolar bone in Group A was significantly thinner than that in Group B and Group C at 1 and 3 mm apically from the alveolar crest. The alveolar width at 1 mm in Group C (60.99 ± 15.36%) was significantly thicker than that in Group B (39.75 ± 30.18%). Histomorphmetrical measurements showed that the buccal alveolar width at 1 mm was significantly thicker in Groups B and C than in Group A. Additionally, buccal bone height and lingual bone width at 1 mm in Group C (87.06 ± 10.34%, 89.09 ± 10.56%) were significantly greater than in Group A (53.48 ± 23.94%, 82.72 ± 12.59%).

Conclusion: The present findings indicate that application of bovine bone combined with barrier membrane with or without FGF-2 over tooth sockets can effectively reduce ridge absorption, especially in terms of ridge width and FGF-2 modified membrane seems to improve the outcomes obtained with membrane alone.
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http://dx.doi.org/10.1111/clr.13749DOI Listing
March 2021

Integrated analysis of DNA methylation and gene expression profiles identified S100A9 as a potential biomarker in ulcerative colitis.

Biosci Rep 2020 12;40(12)

Department of Digestive, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University, Urumqi, Xinjiang 830099, P.R. China.

Ulcerative colitis (UC) is a prevalent relapsing-remitting inflammatory bowel disease whose pathogenetic mechanisms remain elusive. In the present study, colonic biopsies samples from three UC patients treated in the Traditional Chinese Medicine Hospital and three healthy controls were obtained. The genome-wide mRNA and lncRNA expression of the samples were profiled through Agilent gene expression microarray. Moreover, the genome-wide DNA methylation dataset of normal and UC colon tissues was also downloaded from GEO for a collaborative analysis. Differential expression of lncRNA (DELs) and mRNAs (DEMs) in UC samples compared with healthy samples were identified by using limma Bioconductor package. Differentially methylated promoters (DMPs) in UC samples compared with controls were obtained through comparing the average methylation level of CpGs located at promoters by using t-test. Functional enrichment analysis was performed by the DAVID. STRING database was applied to the construction of gene functional interaction network. As a result, 2090 DEMs and 1242 DELs were screened out in UC samples that were closely associated with processes related to complement and coagulation cascades, osteoclast differentiation vaccinia, and hemorrhagic diseases. A total of 90 DEMs and 72 DELs were retained for the construction of functional network for the promoters of their corresponding genes were identified as DMPs. S100A9, HECW2, SOD3 and HIX0114733 showed high interaction degrees in the functional network, and expression of S100A9 was confirmed to be significantly elevated in colon tissues of UC patients compared with that of controls by qRT-PCR that was consistent with gene microarray analysis. These indicate that S100A9 could potentially be used as predictive biomarkers in UC.
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http://dx.doi.org/10.1042/BSR20202384DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711060PMC
December 2020

Effects of nursing methods for emergency pci and non-emergency PCI on the treatment of patients with acute myocardial infarction.

J Pak Med Assoc 2020 Sep;70 [Special Issue](9):31-37

Department of Anus & Intestine, Hiser Hospital, Shibei District, Qingdao, Shandong, China.

Objective: To study the effect of emergency nursing methods on the treatment of acute myocardial infarction (AMI).

Methods: A total of 100 patients with AMI were divided into emergency percutaneous coronary intervention (PCI) group (group A, 50 cases) and non-emergency PCI control group (group B, 50 cases). The clinical outcome, average left ventricular ejection fraction (LVEF), angina pectoris, heart failure, and reperfusion arrhythmia after myocardial infarction were compared between the two groups.

Results: The average hospitalization days of emergency PCI group were less than those of the control group, and the incidence of angina pectoris and heart failure after myocardial infarction was lower than that of the control group. The average LVEF of emergency PCI group was higher than that of the control group.

Conclusions: This shows that emergency nursing of AMI can quickly and efficiently dredge the infarcted artery, reduce the occurrence of cardiovascular events after AMI and the average hospitalization days of patients, improve the left ventricular function and prevent heart failure. This method is a very effective treatment for improving the prognosis in patients with AMI.
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September 2020

Three-dimensional finite element analysis of intramedullary nail with different materials in the treatment of intertrochanteric fractures.

Injury 2021 Apr 28;52(4):705-712. Epub 2020 Oct 28.

College of Biomedical Engineering, Taiyuan University of Technology, No.79, Yingze West Road, Taiyuan, Shanxi, 030024, China.

Intramedullary nails are the common treatment options for femoral intertrochanteric fractures. However, aseptic loosening is considered to be one of the primary forms of failure that can be caused by the stress shielding between the bone and implants. The matching in mechanical properties of implant and bone is a key issue to prevent this failure. Polyetheretherketone (PEEK) and Function-graded (FG) materials are widely used in clinical because of their excellent mechanical properties. In this study, to investigate the biomechanical behaviors of intramedullary nails made of Ti-6Al-4V alloy, Stainless Steel (SS), PEEK and two FG materials, three-dimensional finite element models of intertrochanteric fracture femur with intramedullary nail were constructed with ABAQUS. The maximum von Mises stress on the femoral fracture surface fixed by PEEK intramedullary nail was the largest, followed by FG intramedullary nail, which help stimulate bone growth and subsequently reduce fracture healing time. Compared with traditional metal intramedullary nails, PEEK and FG implants might increase von Mises stress along the same path in the proximal femur. The results showed that PEEK and FG intramedullary nails obviously changed the stress distributions in the bone and reduced stress shielding. This finding indicated that PEEK and FG intramedullary nails have the potential to become alternatives to the conventional metal intramedullary nails.
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http://dx.doi.org/10.1016/j.injury.2020.10.102DOI Listing
April 2021

LncRNA CCAT2 promotes proliferation and suppresses apoptosis of colorectal cancer cells.

J BUON 2020 Jul-Aug;25(4):1840-1846

Department of Emergency Surgery, Affiliated Hospital of Qingdao University, Qingdao, China.

Purpose: To investigate the effects of long non-coding ribonucleic acid (lncRNA) colorectal cancer (CRC)-associated transcript 2 (CCAT2) expression on proliferation and apoptosis of colorectal cancer (CRC) cells.

Methods: Data of lncRNA expression in CRC were downloaded from the cancer genome atlas (TCGA) database for differential expression and survival analyses, and real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was employed to analyze the expression level of lncRNA CCAT2 in 80 cases of CRC and adjacent tissues collected as well as normal colorectal cells and CRC cell lines selected. The cells successfully transfected were collected for the detection of the effects on apoptosis and proliferation. Then, immunofluorescence assay was performed to measure the protein expression levels of apoptotic protein markers B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax).

Results: It was found through differential expression analysis that the expression of lncRNA CCAT2 showed a significant difference in CRC tissues, and CRC patients with a high expression level of lncRNA CCAT2 had poor prognosis. Based on the results of qRT-PCR assay, lncRNA CCAT2 was significantly highly expressed in CRC tissues. After transfection with mimic and NC, its expression was obviously higher in mimic group than that in NC group, and cell lines with over-expressed lncRNA CCAT2 were successfully constructed. The flow cytometry results showed that the proportion of apoptotic cells was 5% in mimic group and about 13% in NC group. According to the results of immunofluorescence assay, Bax was mainly located in the cytoplasm, and the fluorescence intensity was decreased significantly in mimic group, indicating that Bax expression was inhibited.

Conclusions: LncRNA CCAT2 is differentially expressed in CRC, and its expression is significantly upregulated in CRC. LncRNA CCAT2 can promote the growth and proliferation and suppress the apoptosis of CRC cells. The changes in lncRNA CCAT2 expression are associated with poor prognosis.
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October 2020

Progranulin inhibits LPS-induced macrophage M1 polarization via NF-кB and MAPK pathways.

BMC Immunol 2020 06 5;21(1):32. Epub 2020 Jun 5.

Department of Periodontology, School of Stomatology, Shandong University, 44 West Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.

Background: Macrophage M1 polarization plays a pivotal role in inflammatory diseases. Progranulin (PGRN) has potential anti-inflammation action, however, the effect of PGRN on macrophage M1 polarization has been poorly studied. Our study aimed to investigate the effect of PGRN on lipopolysaccharide (LPS)-induced macrophage M1 polarization and clarify the underlying mechanisms.

Methods: RAW264.7 cells were polarized to M1 macrophage by LPS with or without recombinant PGRN (rPGRN) and tumor necrosis factor alpha antibody (anti-TNF-α). A cell counting kit-8 assay (CCK-8), flow cytometry, Quantitative Real-Time PCR assay (q-PCR), Western blot assay and enzyme-linked immunosorbent assay (ELISA) were used to determine the effect of different treatments on cell proliferation, expression of surface phenotype marker and expressions and secretion of inflammatory cytokines. The activation of NF-κB/mitogen-activated protein kinase (MAPK) pathways and the nuclear translocation of NF-κB p65 were detected by Western blot and immunofluorescence respectively. THP-1 and primary bone marrow-derived monocytes (BMDMs) were also used to demonstrate effect of PGRN on expressions and secretion of inflammatory cytokines induced by LPS.

Results: In RAW264.7 cells, rPGRN at concentrations below 80 ng/ml significantly promoted cell proliferation in dose dependent fashion. rPGRN significantly inhibited LPS-induced change of phenotype (CD86/CD206 ratio) and function (tumor necrosis factor (TNF-α) and inducible nitric oxide synthase (iNOS) expressions). LPS-stimulated secretion of TNF-α and activated phosphorylation of IKKα/β, IкBα, p65, JNK and p38 and the nucleus translocation of NF-кB p65 were also significantly downregulated by rPGRN. In addition, recombinant TNF-α (rTNF-α) significantly boosted TNF-α and iNOS expression vs the control group. Moreover, anti-TNF-α significantly inhibited LPS-induced TNF-α and iNOS expression. In THP-1 and BMDM cells, reversing effect of rPGRN on LPS-enhanced expressions of TNF-α and iNOS and secretion of TNF-α was further demonstrated.

Conclusions: PGRN down-regulates LPS-induced macrophage M1 polarization in phenotype and function via NF-κB/MAPK signaling pathways.
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http://dx.doi.org/10.1186/s12865-020-00355-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275413PMC
June 2020

Eosinophilic colitis in a boy with a novel XIAP mutation: a case report.

BMC Pediatr 2020 04 18;20(1):171. Epub 2020 Apr 18.

Department of Gastroenterology, Children's Hospital of Nanjing Medical University, No. 72 Guangzhou Road, Nanjing, Jiangsu Province, 210008, China.

Background: X-linked inhibitor of apoptosis (XIAP) deficiency is a rare primary immunodeficiency disease characterized by haemophagocytic lymphohistiocytosis, recurrent splenomegaly and inflammatory bowel disease (IBD). The only curative treatment is haematopoietic stem cell transplant (HSCT).

Case Presentation: Here, we report the case of a 22-month-old male with a long history of abdominal distension and anaemia. Clinical and laboratory findings were consistent with eosinophilic colitis. To identify the underlying disease, we performed exome sequencing, which showed an unreported frameshift mutation in the XIAP gene.

Conclusion: We present eosinophilic colitis as the initial manifestation of XIAP deficiency for the first time in this article, which expands the mutation spectrum and phenotype of this disease.
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http://dx.doi.org/10.1186/s12887-020-02075-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7165398PMC
April 2020

Response of membrane tension to gravity in an approximate cell model.

Theor Biol Med Model 2019 12 5;16(1):19. Epub 2019 Dec 5.

Key Laboratory for Space Biosciences & Biotechnology, Faculty of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, China.

Background: Gravity, especially hypergravity, can affect the morphology of membranes, and further influence most biological processes. Since vesicle structures are relatively simple, the vesicle can be treated as a vital model to study the mechanical properties of membranes in most cases. Basic research on membrane tension has become a vital research topic in cellular biomechanics.

Methods: In this study, a new vesicle model is proposed to quantitatively investigate the response of membrane tension to gravity. In the model, the aqueous lumen inside the vesicle is represented by water, and the vesicle membrane is simplified as a closed, thin, linear elastic shell. Then, the corresponding static equilibrium differential equations of membrane tension are established, and the analytical expression is obtained by the semi-inverse method. The model parameters of the equations are accurately obtained by fitting the reported data, and the values calculated by the model agree well with the reported results.

Results: The results are as follows: First, both the pseudo-ellipsoidal cap and the pseudo-spherical cap can be used to describe the deformed vesicle model; however, the former can better represent the deformation of the vesicle model because the variance of the pseudo-ellipsoidal cap is smaller. Second, the value of membrane tension is no longer a constant for both models. Interestingly, it varies with the vesicle height under the action of gravity. The closer it is to the substrate, the greater the membrane tension. Finally, the inclination between the tangent and the radial lines at a certain point is nearly proportional to the radius of the cross section in both models.

Conclusion: These findings may be helpful to study the vesicle model spreading more accurately by taking into account the influence of gravity because it could affect the distribution of membrane tension. Furthermore, it may also provide some guidance for cell spreading and may have some implications for membrane tension-related mechanobiology studies, especially in the hypergravity conditions.
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http://dx.doi.org/10.1186/s12976-019-0116-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894217PMC
December 2019

Solute carrier family 25 member 20 mutations may cause low-gamma-glutamyltransferase cholestasis without metabolic crisis in one Chinese infant.

Acta Paediatr 2020 03 14;109(3):623-624. Epub 2019 Oct 14.

Department of Gastroenterology, Children's Hospital Affiliated to Nanjing Medical University, Nanjing, China.

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http://dx.doi.org/10.1111/apa.15034DOI Listing
March 2020

Association of blood eosinophilia and vitamin D insufficiency in young infants with cow milk allergy.

Asia Pac J Clin Nutr 2019 ;28(3):550-557

Children's Hospital of Nanjing Medical University, Nanjing, China.

Background And Objectives: Cow milk allergy is the most common food allergic disease in young infants and vitamin D has a critical role in regulating intestinal inflammation.

Methods And Study Design: To determine roles of vitamin D in cow milk allergy, fifty-six young infants with cow milk allergy were enrolled. The serum 25-hydroxyvitamin D (25OHD), total and specific IgE, circulating regulatory T lymphocytes, and blood eosino-phil counts were determined.

Results: The serum 25OHD in cow milk allergy and age-matched infants were sim-ilar (68.3±38.9 nmol/L versus 72.9±33.1 nmol/L, p>0.05), 71% Cow milk allergy infants (40/56) had serum 25OHD lower than 75 nmol/L compared to 66% (37/56) in the controls. The cow milk allergy infants with 25OHD lower than 75 nmol/L had persistent blood eosinophilia and delayed resolution of symptoms after cow milk elimination compared to those with 25OHD above 75 nmol/L (odd ratio 3.7, 95% CI 1.1-12.6, p<0.05). The serum 25OHD inversely correlated with blood eosinophil counts after cow milk elimination (r=-0.37, p<0.01). Cow milk allergy infants with 25OHD lower than 50 nmol/L (vitamin D deficiency, n = 22) were in general at younger age (1.6±0.6 months) compared to infants with insufficient (50-75 nmol/L) or normal (>=75 nmol/L) group (4.3±1.2 and 4.6±0.9 months, respectively, p<0.001).

Conclusions: Low serum vitamin D associates with persistent blood eosinophilia and symptoms in young cow milk allergy infants.
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http://dx.doi.org/10.6133/apjcn.201909_28(3).0014DOI Listing
February 2020

CFLAR is a critical regulator of cerebral ischaemia-reperfusion injury through regulating inflammation and endoplasmic reticulum (ER) stress.

Biomed Pharmacother 2019 Sep 1;117:109155. Epub 2019 Jul 1.

Department of Internal Medicine, Ankang City Center Hospital, Ankang, Shaanxi 725000, China. Electronic address:

Stroke is a leading cause of mortality and disability globally. Cerebral ischaemia-reperfusion (I/R) injury is characterized by significant inflammation and extensive cell death. Multiple signaling pathways play essential roles in the process, and identifying the unclear crucial regulators of these pathways may provide promising targets for treatment. CASP8 and FADD-like apoptosis regulator (CFLAR) is expressed in multiple organs to regulate inflammation. Here, we reported that CFLAR expression was markedly reduced in brain samples of mice with middle cerebral artery occlusion (MCAO) stroke. Furthermore, CFLAR knockdown markedly elevated the neurological deficit, brain water content and the infarct volume. In addition, significantly promoted inflammation and endoplasmic reticulum (ER) stress was detected in brain tissues of mice after MCAO, as evidenced by the promoted expression of p-IκBα, p-nuclear factor (NF)-κB (p65), glucose-regulated protein 78 (GRP78), PKR-like ER kinase (PERK), activating transcription factor-6 (ATF-6) and cleaved Caspase-12. Notably, MCAO-induced cerebral I/R injury was markedly alleviated in mice over-expressing CFLAR through suppressing inflammation and ER stress. Furthermore, our in vitro results indicated that oxygen-glucose deprivation (OGD)-induced cell death was evidently ameliorated by CFLAR over-expression. In contrast, the cell death triggered by OGD was accelerated by CFLAR knockdown in vitro through enhancing Caspase-3 cleavage, and this effect was obviously ameliorated by the blockage of ER stress using 4-phenyl butyric acid (4-PBA). Collectively, these results demonstrated that CFLAR could be considered as a novel candidate to develop effective therapeutic treatment against cerebral I/R injury.
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http://dx.doi.org/10.1016/j.biopha.2019.109155DOI Listing
September 2019

In vivo experimental study on the resistance and stiffness of orbital suspension tissues with/without the extraocular muscles.

Biomed Eng Online 2019 May 31;18(1):68. Epub 2019 May 31.

Shanxi Key Laboratory of Material Strength & Structural Impact, College of Biomedical Engineering, Taiyuan University of Technology, 79 YingZe West Street, Taiyuan, 030024, Shanxi, China.

Background: The accuracy of the surgical amount of extraocular muscle (EOM) is key to the success of strabismus surgery. To establish an accurate eye movement model, it is of great theoretical value and clinical significance to determine the surgical amount of EOM. At present, only resistance and stiffness data of orbital suspension tissues with EOMs exist, while those of orbital suspension tissues without EOMs, which is critical information for eye movement modeling, have not been reported. The aim of this research is to study the resistance and stiffness of orbital suspension tissues with/without EOMs.

Methods: Fifteen healthy New Zealand white rabbits with body weights of 2.41 ± 0.13 kg were used in the study. Two recti (two horizontal recti of the left eye or two vertical recti of the right eye) or all EOMs were detached from each eye under general anesthesia. Then, a 5-0 silk suture was attached to the stump of the detached rectus insertion (two horizontal recti insertions of the left eye and two vertical recti insertions of the right eye) on the isolated eyeball. The 5-0 silk suture was connected to the INSTRON 5544 tester to facilitate the horizontal rotations of the left eyes and the vertical rotations of the right eyes, respectively.

Results: The resistance and stiffness of orbital suspension tissues with superior rectus, inferior rectus, superior oblique, and inferior oblique EOMs were obtained during horizontal eye movement. Similarly, the resistance and stiffness of orbital suspension tissues with lateral rectus, medial rectus, superior oblique, and inferior oblique EOMs were obtained during vertical eye movement. Then, the resistance and stiffness of orbital suspension tissues without EOMs were obtained during horizontal and vertical eye movements. The resistance and stiffness data of orbital suspension tissues with EOMs were compared with those of orbital suspension tissues without EOMs. The comparison results showed no significant difference in the resistance values between these two cases. In addition, the stiffness values of these two cases statistically differed.

Conclusions: The two horizontal recti play a major role in passive horizontal eye movement. In addition, when the eye is passively moved vertically, the two vertical recti play major roles. The stiffness of orbital suspension tissues with EOMs, which has been used in eye movement modeling, is not accurate. The results of this work may serve as a reference for improving the accuracy in eye movement modeling, and then it will be beneficial for determining the surgical amount of EOMs in clinical surgery.
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http://dx.doi.org/10.1186/s12938-019-0688-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544998PMC
May 2019

The biological behavior optimization of human periodontal ligament stem cells via preconditioning by the combined application of fibroblast growth factor-2 and A83-01 in in vitro culture expansion.

J Transl Med 2019 02 28;17(1):66. Epub 2019 Feb 28.

Department of Periodontology, School of Stomatology, Shandong University, 44 West Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.

Background: As the optimal source of seed cells in periodontal tissue engineering, periodontal ligament stem cells (PDLSCs) have always been researched to improve cell expansion due to their limited resource and spontaneous differentiation in vitro cultivation. Fibroblast growth factor-2 (FGF-2) has been proven to stimulate bone marrow mesenchymal stem cells (BMMSCs) proliferation and maintain their pluripotency when being added to the culture medium. As a small molecule inhibitor of transforming growth factor-beta receptors (TGF-βRs), A83-01 can also promote cell proliferation. Therefore, the aim of this study was to verify whether the combined application of FGF-2 and A83-01 could augment cell quantity and quality during in vitro culture.

Methods: PDLSCs were preconditioned with A83-01, FGF-2, or their combination. A cell counting kit-8 (CCK8) assay, cell apoptosis assay, ALP activity assay, Alizarin Red S staining assay, RT-PCR assay, Western blot assay and ELISA were used to determine the sustained effects of different preconditioning strategies on the proliferation, apoptosis, stemness, osteogenic differentiation and paracrine action of PDLSCs.

Results: The combined application of FGF-2 and A83-01 significantly augmented cell expansion, reduced cell apoptosis, magnified stemness expression, promoted later osteogenic differentiation and mineralization and increased paracrine action of PDLSCs compared with the control. Moreover, the combination presented significant advantages in enhancing proliferation, stemness expression and paracrine action over FGF-2 alone.

Conclusions: The combined application of A83-01 and FGF-2 may be an improved strategy for PDLSCs biological behavior optimization in culture expansion and advantageous for reinforcing proliferation, stemness expression and cytokine secretion over FGF-2 alone.
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http://dx.doi.org/10.1186/s12967-019-1799-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396448PMC
February 2019

Transcriptome analysis and discovery of genes involved in immune pathways in Solen strictus (Gould, 1861) under Vibrio anguillarum.

Fish Shellfish Immunol 2019 May 18;88:237-243. Epub 2019 Feb 18.

Department of Animal Nutrition and Aquaculture, Shandong Vocational Animal Science and Veterinary College, 88 Shengli East Street, Weifang 261061, China. Electronic address:

The Solen strictus (Gould, 1861) has been recognized as an important marine economic bivalve in Eastern and Southeast Asia. To gain a better understanding of the S. strictus immune system and its related genes in response to bacterial infections, we performed a comparative gene transcription analysis from S. strictus with Vibrio anguillarum through RNA-Seq technology, meanwhile the differentially expressed genes (DEGs) were investigated. After assembly, a total of 195,774 transcripts with an average length of 996 bp were obtained. Total 153,038 unigenes were annotated in the nr, Swiss-Prot, KEGG, COG, KOG, GO and Pfam databases, and 56,597 unigenes (36.98%) were annotated in at least one database. After bacterial challenge, there were 1588 significant differentially expressed genes (DEGs) between the challenged and control groups, including 999 up-regulated and 589 down-regulated genes. All the DEGs were classified into three gene ontology categories, and allocated to 225 KEGG pathways. Immune-related genes were detected from immune system pathways among the top 20 enriched pathways, such as Toll-like receptor signaling, RIG-I-like receptor signaling and NOD-like receptor signaling pathway. In addition, 56,079 potential simple sequence repeats (SSRs) and 1,031,521 candidate single nucleotide polymorphisms (SNPs) were detected and identified in the S. strictus transcriptome. Results of the present study will provide valuable theoretical resources for future genetic and genomic research on S. strictus. The research results will be helpful for improving the efficiency and quality of artificial breeding, establishing genetic linkage map, and enhancing health management for this species.
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http://dx.doi.org/10.1016/j.fsi.2019.01.024DOI Listing
May 2019

Abnormal thymic stromal lymphopoietin expression in the gastrointestinal mucosa of patients with eosinophilic gastroenteritis.

J Pediatr (Rio J) 2020 May - Jun;96(3):350-355. Epub 2019 Feb 11.

Children's Hospital Affiliated to Nanjing Medical University, Department of Gastroenterology, Nanjing, China. Electronic address:

Objective: To investigate the differential expression of the thymic stromal lymphopoietin isoforms, short and long, and discern their biological implications under eosinophilic gastroenteritis.

Methods: The expression of thymic stromal lymphopoietin and its two isoforms in tissues was assessed by quantitative RT-PCR in healthy controls (n=24) and patients with eosinophilic gastroenteritis (n=17).

Results: Thymic stromal lymphopoietin mRNA was significantly reduced in eosinophilic gastroenteritis when compared with healthy controls (p<0.0001). A significantly lower amount of short thymic stromal lymphopoietin mRNA was observed in eosinophilic gastroenteritis when compared with controls (p<0.05), while a significantly higher amount of long thymic stromal lymphopoietin mRNA was observed in eosinophilic gastroenteritis when compared with controls (p<0.05). Peak eosinophilic count is significantly positively correlated with the expression of long thymic stromal lymphopoietin mRNA in the gastrointestinal mucosal of patients with eosinophilic gastroenteritis (r=0.623, p<0.005), while peak eosinophilic count is significantly negatively correlated with the expression of short thymic stromal lymphopoietin mRNA in the gastrointestinal mucosal of patients with eosinophilic gastroenteritis (r=-0.4474, p<0.05).

Conclusions: Abnormal mucosal thymic stromal lymphopoietin expression may contribute to gastrointestinal mucosa damage in eosinophilic gastroenteritis.
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http://dx.doi.org/10.1016/j.jped.2018.11.012DOI Listing
October 2020

Progranulin Promotes Regeneration of Inflammatory Periodontal Bone Defect in Rats via Anti-inflammation, Osteoclastogenic Inhibition, and Osteogenic Promotion.

Inflammation 2019 Feb;42(1):221-234

Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, Shandong, China.

Progranulin (PGRN) has been proved to play a crucial role in anti-inflammation and osteogenesis promotion; thus, it was hypothesized that PGRN could promote bone regeneration in periodontal disease. In this experiment, the periodontal bone defects were established in periodontitis rats; recombinant human progranulin (rhPGRN), tumor necrosis factor alpha inhibitor (anti-TNF-α), or phosphate buffer saline (PBS)-loaded collagen membrane scaffolds were implanted within defects and the rats were sacrificed at scheduled time points. Volume of new bone was assessed by radiological and histomorphometric analyses. Expression of osteogenesis-related markers and tumor necrosis factor-α (TNF-α) was evaluated using immunohistochemistry. Tartrate-resistant acid phosphatase (TRAP) staining was also performed to determine the number of osteoclasts. Immunofluorescence (IF) staining was performed to explore the interaction between rhPGRN and tumor necrosis factor receptors (TNFRs). The results showed that the rhPGRN group had significantly superior quantity and quality of newly formed bone, higher expression of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and TNFR2 compared with the PBS group and the anti-TNF-α group. Similarly to the anti-TNF-α group, the rhPGRN group also exhibited the significant inhibitory effect on the expression of TNF-α and the number of TRAP-positive cells compared with the PBS group. Hence, our experiment suggests that PGRN promotes regeneration of inflammatory periodontal bone defect in rats via anti-inflammation, osteoclastogenic inhibition, and osteogenic promotion. Local administration of PGRN may provide a new therapeutic strategy for periodontal bone regeneration.
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http://dx.doi.org/10.1007/s10753-018-0886-4DOI Listing
February 2019

A Novel Protein Elicitor BAR11 From Hhs.015 Improves Plant Resistance to Pathogens and Interacts With Catalases as Targets.

Front Microbiol 2018 9;9:700. Epub 2018 Apr 9.

State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, China.

Previously, we reported the biocontrol effects of strain Hhs.015 on . Here, we report a novel protein elicitor BAR11 from the biocontrol strain Hhs.015 and its functions in plant defense responses. Functional analysis showed that the elicitor BAR11 significantly stimulated plant systemic resistance in to pv. DC3000. In addition, systemic tissues accumulated reactive oxygen species and deposited callose in a short period post-treatment compared with the control. Quantitative RT-PCR results revealed that BAR11 can induce plant resistance through the salicylic acid and jasmonic acid signaling pathways. Further analysis indicated that BAR11 interacts with host catalases in plant cells. Taken together, we conclude that the elicitor BAR11 from the strain Hhs.015 can trigger defense responses in plants.
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http://dx.doi.org/10.3389/fmicb.2018.00700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5900052PMC
April 2018

Periodontitis May Restrain the Mandibular Bone Healing via Disturbing Osteogenic and Osteoclastic Balance.

Inflammation 2018 Jun;41(3):972-983

Department of Periodontology, School of Dentistry, Shandong University, Jinan, Shandong, 250000, China.

Periodontitis has been advocated as a systematic chronic low-grade infection burden. However, the relationship between periodontitis and bone defect healing has not been elucidated. One hundred and eight male Wister rats were randomly assigned into three groups: control (healthy) group, periodontitis group, and periodontitis plus human tumor necrosis factor receptorII:IgG Fc fusion protein (rhTNFR:Fc) group. The experimental periodontitis model was established by ligaturing with orthodontic wire and silk suture plus local administration of 20 μl of lipopolysaccharide (LPS). Mandibular bone defects in size of 4 × 2 × 1 mm were created for all the rats and rhTNFR:Fc subcutaneously injected at neck at a dose of 2.5 mg/kg every 3 days for the periodontitis plus rhTNFR:Fc group. The gene and protein expressions of bone-related markers in the healing tissue were monitored and new bone formation was histologically evaluated. Tartrate-resistant acid phosphatase (TRAP) staining was performed to determine the number of osteoclasts. The results showed that the mRNA and protein expressions of osteogenesis-related markers were significantly lower while nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) gene expression was significantly higher in the periodontitis group. The periodontitis group showed decreased new bone formation and increased number of osteoclasts when compared to the control group. However, there was no significant difference between the periodontitis plus rhTNFR:Fc group and the control group. These results demonstrated that periodontitis may restrain the mandibular bone healing via disturbing osteogenic and osteoclastic balance in which tumor necrosis factor-α (TNF-α) could act as a leverage.
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http://dx.doi.org/10.1007/s10753-018-0751-5DOI Listing
June 2018

Prevalence of hyperhomocysteinemia and its associated factors in patients with primary hypertension in Chinese urban communities: A cross-sectional study from Nanjing.

Clin Exp Hypertens 2018 27;40(5):495-500. Epub 2017 Nov 27.

a Chronic Disease and Health Management Research Center, the Geriatrics Institute of Jiangsu Province , Nanjing , China.

Objective: This study aimed to investigate the prevalence of hyperhomocysteinemia (Hhcy) and its major determinants in Chinese urban population with new-onset hypertension.

Methods: A total of 574 adults (404 men and 170 women) with newly diagnosed primary hypertension were recruited from seven communities in Nanjing, China. Data on lifestyle factors, such as physical activities, current smoking and drinking status, dietary habits, and familial factors were collected in interviews, and laboratory examinations were performed by well-trained personnel. Potential factors related to the prevalence of Hhcy in this population were analyzed using a logistic regression model.

Results: Of the 574 participants, 421 (73.3%) were diagnosed with Hhcy whereas the remainder were only hypertensive. The study highlighted a number of factors that were significantly correlated (p < 0.001) with the prevalence of Hhcy. Subjects with Hhcy were more likely to be male (odds ratio [OR] 3.007, 95% confidence interval [CI] 1.781-5.079), have a smoking history (OR 1.052, 95% CI 1.031-1.074), older (OR 1.052, 95% CI 1.031-1.074), have an elevated Body Mass Index BMI (OR 1.160, 95% CI 1.080-1.246) and higher levels of low density lipoprotein cholesterol (LDL-c) (OR 1.590, 95% CI 1.226-2.063). Regular and adequate physical activity was associated with normal homocysteine levels in both male and female groups (p < 0.05). For males only, having a higher BMI, higher LDL-c or being older significantly (p < 0.05) affected the chances of Hhcy. Whereas for females, lower levels of eGFR could be related to Hhcy (p < 0.05).

Conclusions: Our present study reported a high prevalence of Hhcy in the Nanjing population with new-onset hypertension. Associated factors like physical activity, gender, smoking history, age, BMI, and LDL-c were important modifiers of plasma homocysteine concentration. Management and intervention of the above associated factors should be implicated to improve H type hypertension control.
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http://dx.doi.org/10.1080/10641963.2017.1403621DOI Listing
September 2018

miR-15b inhibits cancer-initiating cell phenotypes and chemoresistance of cisplatin by targeting TRIM14 in oral tongue squamous cell cancer.

Oncol Rep 2017 May 27;37(5):2720-2726. Epub 2017 Mar 27.

Department of Surgery, University of California Davis, Sacaramento, CA 95817, USA.

Oral tongue squamous cell carcinoma (TSCC) is one of the most lethal cancers within the oral cavity and its prognosis remains dismal due to the paucity of effective therapeutic targets. The formation of cancer-initiating cells (CICs) and epithelial-mesenchymal transition (EMT) are pivotal events involved in the dismal prognosis. They have been shown to be related to the resistance to cisplatin treatment. In the present study, we showed that TRIM14 induced formation of cancer-initiating cells and EMT in TSCC SCC25 cells. Its overexpression promoted cisplatin resistance in the SCC25 cells. We found that overexpression of miR-15b suppressed TRIM14 and inhibited CIC phenotypes in the SCC25 cells. Moreover, overexpression of miR-15b promoted mesenchymal-epithelial transition (MET) in the SCC25 cells and sensitized cisplatin-resistant SCC25 (SCC25-res) cells to cisplatin. Thus, we conclude that miR-15b inhibited cancer stem cell phenotypes and its restoration reversed the chemoresistance of cisplatin by targeting TRIM14 in TSCC. Elucidating the molecular mechanism of EMT and cancer stem cells in TSCC may further aid in the understanding of the pathogenesis and progression of the disease, and offer novel targets for the discovery of new drugs.
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http://dx.doi.org/10.3892/or.2017.5532DOI Listing
May 2017

A novel missense mutation Q495K of SLC26A3 gene identified in a Chinese child with congenital chloride-losing diarrhoea.

Acta Paediatr 2017 Jun 19;106(6):1004-1005. Epub 2017 Apr 19.

Department of Gastroenterology, Children's Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu Province, China.

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http://dx.doi.org/10.1111/apa.13811DOI Listing
June 2017

The biomechanical significance of pulley on binocular vision.

Biomed Eng Online 2016 Dec 28;15(Suppl 2):137. Epub 2016 Dec 28.

Shanxi Key Laboratory of Material Strength and Structural Impact, Institute of Applied Mechanics and Biomedical Engineering, College of Mechanics, Taiyuan University of Technology, Yingze West Street 79, Taiyuan, 030024, Shanxi, China.

Background: Pulleys have been reported as the functional origins of the rectus extraocular muscles (EOMs). However, biomechanical significance of pulleys on binocular vision has not been reported.

Methods: Three eye movement models, i.e., non-pulley model, passive-pulley model, and active-pulley model, are used to simulate the horizontal movement of the eyes from the primary position to the left direction in the range of 1°-30°. The resultant forces of six EOMs along both orthogonal directions (i.e., the x-axis and y-axis defined in this paper) in the horizontal plane are calculated using the three models.

Results: The resultant force along the y-axis of the left eye for non-pulley model are significantly larger than that of the other two pulley models. The difference of the force, between the left eye and the right eye in non-pulley model, is larger than those in the other two pulley models along x-axis and y-axis.

Conclusion: The pulley models present more biomechanical advantage on the horizontally binocular vision than the non-pulley model. Combining with the previous imaging evidences of pulleys, the results show that pulley model coincides well with the real physiological conditions.
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http://dx.doi.org/10.1186/s12938-016-0280-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5260023PMC
December 2016

Corrigendum to "Imbalance of peripheral blood Th17 and Treg responses in children with refractory Mycoplasma pneumoniae pneumonia" [J Infect Chemother 22 (2016) 162-166].

J Infect Chemother 2017 03 20;23(3):193. Epub 2017 Jan 20.

Department of Gastroenterology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, China.

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http://dx.doi.org/10.1016/j.jiac.2016.12.005DOI Listing
March 2017

The Protective effect of the endoplasmic reticulum stress-related factors BiP/GRP78 and CHOP/Gadd153 on noise-induced hearing loss in guinea pigs.

Noise Health 2016 Sep-Oct;18(84):247-255

Department of Otorhinolaryngology, Head and Neck Surgery of Zhongnan Hospital, Wuhan University, Wuhan, Hubei, China; Laboratory of Immunology, Faculty of Medicine, University of Lorraine, Vandoeuvre-Les-Nancy, Nancy, France.

Context: The audiological features and cochlear morphology of individuals with noise-induced hearing loss (NIHL) are well characterized. However, the molecular processes in the cochlea are not well understood.

Aims: To explore the role of the endoplasmic reticulum stress (ERS) response in the guinea pig model of cochlear damage induced by exposure to intense noise.

Settings And Design: A pilot case-control study.

Subjects And Methods: Forty-eight guinea pigs were divided into four equal groups. At 1, 4, or 14 days (d) post-exposure, the auditory brainstem responses (ABRs) were tested before sacrificing the subjects. The expression levels of the binding immunoglobulin protein/glucose-regulated protein 78 (BiP/GRP78) and C/EBP-homologous protein/growth arrest and DNA damage-inducible gene 153 (CHOP/Gadd153) proteins were evaluated using immunohistochemistry and Western blotting. The number of cochlear hair cells with altered nuclei was counted using confocal fluorescence microscopy.

Statistical Analysis Used: One-way analysis of variance (ANOVA) and the least squares difference (LSD) test.

Results: The outer hair cells (OHCs) showed changes of apoptosis, necrosis, and loss after noise exposure. In the 1- and 4-d groups, more apoptotic cells were found than necrotic cells (P < 0.01). The level of BiP/GRP78 was significantly higher in all three experimental groups compared to the control group (P < 0.01). The level of CHOP/Gadd153 was increased at 1 d post-exposure, achieving a peak that was maintained until 4 d, after which it returned to baseline levels by 14 d post-exposure.

Conclusions: ERS response was activated by inducing the expression of BiP/GRP78 to lessen the extent of the resulting cellular damage and activating the CHOP/Gadd153 pathway to eliminate the most severely damaged cells.
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http://dx.doi.org/10.4103/1463-1741.192481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187652PMC
June 2017

Generation of Induced Pluripotent Stem Cells with Substitutes for Yamanaka's Four Transcription Factors.

Cell Reprogram 2016 10 20;18(5):281-297. Epub 2016 Jul 20.

1 College of Animal Science and Technology, Southwest University , Chongqing, China .

Induced pluripotent stem cells (iPSCs) share many characteristics with embryonic stem cells, but lack ethical controversy. They provide vast opportunities for disease modeling, pathogenesis understanding, therapeutic drug development, toxicology, organ synthesis, and treatment of degenerative disease. However, this procedure also has many potential challenges, including a slow generation time, low efficiency, partially reprogrammed colonies, as well as somatic coding mutations in the genome. Pioneered by Shinya Yamanaka's team in 2006, iPSCs were first generated by introducing four transcription factors: Oct 4, Sox 2, Klf 4, and c-Myc (OSKM). Of those factors, Klf 4 and c-Myc are oncogenes, which are potentially a tumor risk. Therefore, to avoid problems such as tumorigenesis and low throughput, one of the key strategies has been to use other methods, including members of the same subgroup of transcription factors, activators or inhibitors of signaling pathways, microRNAs, epigenetic modifiers, or even differentiation-associated factors, to functionally replace the reprogramming transcription factors. In this study, we will mainly focus on the advances in the generation of iPSCs with substitutes for OSKM. The identification and combination of novel proteins or chemicals, particularly small molecules, to induce pluripotency will provide useful tools to discover the molecular mechanisms governing reprogramming and ultimately lead to the development of new iPSC-based therapeutics for future clinical applications.
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http://dx.doi.org/10.1089/cell.2016.0020DOI Listing
October 2016

A Rapid and Sensitive UPLC-MS/MS Method for Determination of Docetaxel in Rabbit Plasma: Pharmacokinetic Study of New Lung-Targeting Docetaxel Liposome at Low Dose.

Cell Biochem Biophys 2015 Dec;73(3):623-9

College of Pharmacy, Chongqing Medical University, No. 1 Yixue Yuan Road, Chongqing, 400016, People's Republic of China.

In this study, a more rapid and more sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method is developed and validated for the pharmacokinetic study of a new lung-targeting docetaxel liposome (DTX-LP) at low dose (1 mg/kg) in rabbits. The method is reliable and reproducible with intra-day precision below 5.75 %, inter-day precision below 7.57 %, and mean extraction recovery of 84.1-91.7 %. At low dose, the validated method is successfully applied to the comparative pharmacokinetic study of docetaxel (DTX) in rabbit plasma after intravenous administration of DTX-LP and DTX injection, respectively. The results indicate that the pharmacokinetic profile of DTX is completely changed when loaded in the new type of liposome, which can make DTX quicker distribution from the circulation to the target organ and slower eliminated from the body.
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http://dx.doi.org/10.1007/s12013-015-0639-zDOI Listing
December 2015

Optimal kicking of a trampolinist.

Hum Mov Sci 2016 Aug 28;48:54-61. Epub 2016 Apr 28.

Institute of Applied Mechanics and Biomedical Engineering, Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address:

This work aims to create a mathematical model by using kinematical equations that can describe the landing-jump of a trampoline performance. In this model, the trampolinist was assumed to be a combination of two parts of masses. The landing process (from the time trampolinist touches the net to the deepest location) was discretized into two stages. During these two stages, the trampolinist exerts different internal forces. Analyzing the kinematics of the abovementioned two stages, we obtained the numerical solutions for the optimum loading time and loading force of the trampolinist to get the deepest landing location. This work has potential for guiding a trampolinist in developing his/her personalized optimal strategy for exerting force during landing on the trampoline.
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http://dx.doi.org/10.1016/j.humov.2016.04.005DOI Listing
August 2016

Contractile Force of Human Extraocular Muscle: A Theoretical Analysis.

Appl Bionics Biomech 2016 17;2016:4091824. Epub 2016 Mar 17.

College of Mechanics, Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, China.

Aim. The length-contractile force relationships of six human extraocular muscles (EOMs) in primary innervations should be determined during eye movement modeling and surgery of clinical EOMs. This study aims to investigate these relationships. Method. The proposal is based on the assumption that six EOMs have similar constitutive relationships, with the eye suspended in the primary position. The constitutive relationships of EOMs are obtained by optimizing from previous experimental data and the theory of mechanical equilibrium using traditional model. Further, simulate the existing experiment of resistance force, and then compare the simulated results with the existing experimental results. Finally, the mechanical constitutive relationships of EOMs are obtained. Results. The results show that the simulated resistance forces from the other four EOMs except for the horizontal recti well agree with previous experimental results. Conclusion. The mechanical constitutive relationships of six EOMs in primary innervations are obtained, and the rationality of the constitutive relationships is verified. Whereafter, the active stress-strain relationships of the six EOMs in the primary innervations are obtained. The research results can improve the eye movement model to predict the surgical amounts of EOMs before EOM surgery more precisely.
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http://dx.doi.org/10.1155/2016/4091824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814899PMC
April 2016