Publications by authors named "Hongling Yang"

48 Publications

Plasma SerpinA5 in conjunction with uterine artery pulsatility index and clinical risk factor for the early prediction of preeclampsia.

PLoS One 2021 14;16(10):e0258541. Epub 2021 Oct 14.

Department of Clinical Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, Guangdong, China.

Object: This study aimed to combine plasma protein SerpinA5 with uterine artery doppler ultrasound and clinical risk factor during the first trimester for prediction of preeclampsia.

Methods And Materials: This study was a nested cohort study and was divided into the screening set and developing set. The plasma was collected during the first trimester (11+0-13+6 weeks), at the same time, UtA-PI was detected and recorded with four-dimensional color Doppler ultrasound. These pregnancies were followed up until after delivery. The plasma proteins were examined using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and enzyme linked immunosorbent assay (ELISA). Placental samples preserved after delivery were analysed by immunohistochemistry. Clinical risk factors were obtained from medical records or antenatal questionnaires. Upregulation or downregulation of SerpinA5 expression in TEV-1 cells was performed to investigate the role of SerpinA5 in trophoblasts invasion.

Results: We demonstrated that SerpinA5 levels were greater not only in preeclampsia placental tissue but also in plasma (both p<0.05), and we found that SerpinA5 may interfere with trophoblastic cell invasion by inhibiting MSP. SerpinA5 may be a potential predictor of preeclampsia. What is more, the sensitivity and specificity of predictive power were strengthened when plasma SerpinA5 was combined with UtA-PI and pre-pregnancy BMI & family history of PE for prediction of preeclampsia.

Conclusion: These findings showed that placenta-derived plasma SerpinA5 may be a novel biomarker for preeclampsia, which together with uterine artery Doppler ultrasound and clinical risk factor can more effectively predict preeclampsia.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258541PLOS
October 2021

Highly Sensitive Fluorescence Detection of Global 5-Hydroxymethylcytosine from Nanogram Input with Strongly Emitting Copper Nanotags.

Anal Chem 2021 Oct 12. Epub 2021 Oct 12.

School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China.

Quantitative analysis of 5-hydroxymethylcytosine (5hmC) has remarkable clinical significance to early cancer diagnosis; however, it is limited by the requirement in current assays for large amounts of starting material and expensive instruments requring expertise. Herein, we present a highly sensitive fluorescence method, termed hmC-TACN, for global 5hmC quantification from several nanogram inputs based on terminal deoxynucleotide transferase (TdT)-assisted formation of fluorescent copper (Cu) nanotags. In this method, 5hmC is labeled with click tags by T4 phage β-glucosyltransferase (β-GT) and cross-linked with a random DNA primer via click chemistry. TdT initiates the template-free extension along the primer at the modified 5hmC site and then generates a long polythymine (T) tail, which can template the production of strongly emitting Cu nanoparticles (CuNPs). Consequently, an intensely fluorescent tag containing numerous CuNPs can be labeled onto the 5hmC site, providing the sensitive quantification of 5hmC with a limit of detection (LOD) as low as 0.021% of total nucleotides (/ = 3). With only a 5 ng input (∼1000 cells) of genomic DNA, global 5hmC levels were accurately determined in mouse tissues, human cell lines (including normal and cancer cells of breast, lung, and liver), and urines of a bladder cancer patient and healthy control. Moreover, as few as 100 cells can also be distinguished between normal and cancer cells. The hmC-TACN method has great promise of being cost effective and easily mastered, with low-input clinical utility, and even for the microzone analysis of tumor models.
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http://dx.doi.org/10.1021/acs.analchem.1c03266DOI Listing
October 2021

The Response of Critical Microbial Taxa to Litter Micro-Nutrients and Macro-Chemistry Determined the Agricultural Soil Priming Intensity After Afforestation.

Front Microbiol 2021 16;12:730117. Epub 2021 Sep 16.

Naiman Desertification Research Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou, China.

Afforestation with trees and shrubs around cropland can effectively decrease soil degradation and avoid sand storms, but subsequent modification of litter quality accelerates the degradation of native organic matter the soil priming effect (PE). Although carbon accumulation in agricultural soils after afforestation was widely studied, little is known about the extent to which soil organic carbon (SOC) mineralization is induced by complex residue input in agro-forest-grass composite ecosystems. Here, we mixed corn field soil and litter of afforestation tree and shrub species together in a micro-environment to quantify the effects of litter-mixture input on farmland soil priming associated with afforestation. Additionally, we studied the responses of bacterial and fungal species to litter chemistry, with the aim to identify the litter and microbial driver of soil priming. The results showed that soil priming was accelerated by different litter addition which varied from 24 to 74% of SOC mineralization, suggesting that priming intensity was relatively flexible and highly affected by litter quality. We also find that the macro-chemistry (including litter carbon, nitrogen, lignin, and cellulose) directly affects priming intensity, while micro-chemistry (including litter soluble sugar, water-soluble phenol, methanol-soluble phenol, and condensed tannin) indirectly influences priming alteration to dominant bacterial taxa. The stepwise regression analysis suggested that litter nitrogen and cellulose were the critical litter drivers to soil priming ( = 0.279), and the combination of bacterial phylum , , , , and fungal taxa was a great model to explain the priming intensity ( = 0.407).
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http://dx.doi.org/10.3389/fmicb.2021.730117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481769PMC
September 2021

Double-site recognition of Staphylococcus aureus using a metal-organic framework material with an alkaline hydrolysis property as a sensitive fluorescent probe.

Nanoscale 2021 Aug 20;13(29):12546-12552. Epub 2021 Jul 20.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.

A metal-organic framework (MOF) material was prepared from 2-aminoterephthalic acid and aluminum chloride with a solvothermal synthesis protocol. The as-prepared MOF material named NH-MIL-53(Al) emitted a very intensive fluorescent (FL) signal after it was hydrolyzed in alkaline solution for releasing numerous FL ligands NH-HBDC. Thus it can be considered as a sensitive FL probe for studying biorecognition events. In this proof-of-principle work, a double-site recognition method was established to quantify Staphylococcus aureus (S. aureus) relying on the alkaline hydrolysis property of the MOF material. In particular, magnetic beads (MBs) modified with pig IgG were adopted for binding S. aureus based on the strong affinity between pig IgG and protein A on the bacterial surface. Meanwhile, MOF NH-MIL-53(Al)-tagged teicoplanin (TEI) was adopted for tracing the target bacteria. By hydrolyzing the MOF material bound on the MBs to trigger the FL signal, S. aureus can be quantified with a dynamic range of 3.3 × 10-3.3 × 10 CFU mL and a detection limit of 5.3 × 10 CFU mL (3σ). The method can exclude efficiently the interference from other common bacteria. It has been applied to quantify S. aureus in saliva, pomegranate green tea, glucose injection and milk samples with satisfactory results, verifying the application potential for analyzing various types of real samples contaminated with S. aureus.
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http://dx.doi.org/10.1039/d1nr02108gDOI Listing
August 2021

Association of self-rated health with chronic disease, mental health symptom and social relationship in older people.

Sci Rep 2021 07 19;11(1):14653. Epub 2021 Jul 19.

Department of Prevention Medicine, School of Public Health, Guangzhou Medical University, Guangzhou, China.

Chronic disease, mental health symptoms and poor social relations are reported common causes for poor self-rated health in older people. To assess the co-occurrence rate of chronic diseases, poor mental health and poor social relationships in older people, and determine their association with self-rated health. 6,551 older people in Zhongshan, China, participated a large health surveillance program were randomly selected and questioned about their SRH, chronic conditions, mental health symptoms and social relationships. The association between self-rated health and chronic conditions, poor mental health, social relationships, and their co-occurrence were analyzed. 56.4% of participants reported poor self-rated health. 39.1% experienced at least one chronic disease. 29.0% experienced one or more mental health symptoms; 19.5% experienced at least one poor social relationship. 7.8% had co-occurrence of chronic diseases, mental health problems, and poor social relationships. Logistic regressions showed that poor self-rated health was associated with chronic diseases, poor mental health, poor social relationships and their co-occurrence. The findings indicate the importance of managing chronic disease, poor mental health and poor social relationships for older people.
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http://dx.doi.org/10.1038/s41598-021-94318-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289838PMC
July 2021

Correlation between the positive rate of SAA in children with respiratory tract infection and ambient temperature.

Environ Sci Pollut Res Int 2021 Jun 24. Epub 2021 Jun 24.

Department of Clinical Laboratory, Guangzhou Women and Children's Medical Centre of Guangzhou Medical University, No. 9, Jinsui Road, Guangzhou, 510623, Guangdong, China.

The purpose of this study was to explore the application of serum amyloid A (SAA) in the outcome of upper respiratory tract infection in children by analyzing the correlation between the change of mean air temperature and the positive rate of SAA detection in children. Daily data on upper respiratory tract infection diseases and weather conditions were collected in 2016-2019. A quasi-Poisson regression with a distributed lag non-linear model was used to examine the association between temperature and SAA-positive rate. The positive rate of SAA had a moderate correlation with the temperature and a weak correlation with relative humidity. Low ambient temperature (7 °C, P) was related to the increase in the positive rate of SAA, with the effect lag for 0-7 days (RR 1.34 (1.19~1.74)). The increase in the SAA-positive case induced by 27 °C (P) could last for 0-14 days (RR 1.07 (1.01-1.08)), and high temperature (30 °C, P) could reduce the positive rate of SAA. Our findings add additional evidence to the adverse effects of sub-optimal ambient temperature and provide useful information for public health programs targeting pediatric patients.
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http://dx.doi.org/10.1007/s11356-021-15033-8DOI Listing
June 2021

Early Prediction Model of Gestational Hypertension by Multi-Biomarkers Before 20 Weeks Gestation.

Diabetes Metab Syndr Obes 2021 31;14:2441-2451. Epub 2021 May 31.

Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, People's Republic of China.

Background: Gestational hypertension (GH), a hypertensive disorder of pregnancy (HDP), is a leading cause of maternal and fetal mortality due to the lack of clarity on its exact etiology and clinically feasible prediction models. This study was performed to discover novel biomarkers before 20 weeks gestation and thereby construct an early GH prediction model.

Methods: This study was designed based on differentially expressed protein screening followed by clinical validation. In the screening phase, a nested case-controlled study was conducted by plasma proteomic analyses using label-free LC-MS/MS and plasma samples from seven pre-GH cases before 20-week gestation and seven age- and gestational week-matched controls. In the validation phase, 10 proteins with differential expression in the screening phase were validated by ELISA or electrochemiluminescence in an independent study consisting of 29 pre-GH cases before 20-week gestation and 29 matched controls.

Results: In the screening phase, 149 proteins were found to be differentially expressed between the two groups and were predominantly involved in complement and coagulation cascades, platelet degranulation and positive regulation of cell motility. Further validation showed that serpin family C member 1 (SERPINC1), serpin family A member 5 (SERPINA5), complement factor H-related protein 5 (CFHR5), clusterin, cytokeratin 18 (CK18) and histidine-rich glycoprotein (HRG) levels were significantly higher in women who later developed GH compared to women with uncomplicated pregnancies (<0.05). Binary logistic regression analysis was used to determine the combination efficacy of models for early prediction of GH. The model with a combination of SERPINC1, CK18 and HRG had a significantly better discriminatory power (AUC = 0.91, 95% CI 0.83-0.98) compared to the models with those proteins alone as independent predictors of GH.

Conclusion: Plasma levels of SERPINC1, SERPINA5, CFHR5, clusterin, CK18 and HRG are potential novel predictive biomarkers of GH, and a prediction model using a combination of SERPINC1, CK18 and HRG has good discriminatory performance for GH before 20 weeks gestation.
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http://dx.doi.org/10.2147/DMSO.S309725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178612PMC
May 2021

Manganese vacancy-confined single-atom Ag in cryptomelane nanorods for efficient Wacker oxidation of styrene derivatives.

Chem Sci 2021 Mar 16;12(17):6099-6106. Epub 2021 Mar 16.

Department of Chemistry, Tsinghua University Beijing 100084 China

Single-atom catalysts provide a pathway to elucidate the nature of catalytically active sites. However, keeping them stabilized during operation proves to be challenging. Herein, we employ cryptomelane-type octahedral molecular sieve nanorods featuring abundant manganese vacancy defects as a support, to periodically anchor single-atom Ag. The doped Ag atoms with tetrahedral coordination are found to locate at cation substitution sites rather than being supported on the catalyst surface, thus effectively tuning the electronic structure of adjacent manganese atoms. The resulting unique Ag-O-MnO unit functions as the active site. Its turnover frequency reaches 1038 h, one order of magnitude higher than for previously reported catalysts, with 90% selectivity for anti-Markovnikov phenylacetaldehyde. Mechanistic studies reveal that the activation of styrene on the ensemble site of Ag-O-MnO is significantly promoted, which can accelerate the oxidation of styrene and, in particular, the rate-determining step of forming the epoxide intermediate. Such an extraordinary electronic promotion can be extended to other single-atom catalysts and paves the way for their practical applications.
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http://dx.doi.org/10.1039/d1sc00700aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098698PMC
March 2021

Serum complement proteomics reveal biomarkers for hypertension disorder of pregnancy and the potential role of Clusterin.

Reprod Biol Endocrinol 2021 Apr 19;19(1):56. Epub 2021 Apr 19.

Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, No.9, Jinsui Road, Guangzhou, 510623, Guangdong, China.

Introduction: Hypertension disorder of pregnancy (HDP) is one of the leading causes of maternal and foetal illness. The aim of the current study was to identify and verify novel serum markers for HDP.

Methods: A label-free LC-MS/MS method was used to establish the serum proteomic profiles of 12 pre-HDP (before clinical diagnosis of HDP) pregnancies and verify prioritized candidates in the verification set of 48 pre-HDP pregnancies. These biomarkers were revalidated by ELISA in an independent cohort of 88 pre-HDP pregnancies. Subsequently, the candidate biomarkers were histologically analysed by immunohistochemistry, and function was evaluated in TEV-1 cells.

Results: We identified 33 proteins with significantly increased abundance and 14 with decreased abundance (peptide FDR ≤ 1%, P < 0.05). Complement was one of the top enriched components in the pre-HDP group compared with the control group. Three complement factors (CLU, CFHR5, and CRP) were significantly increased in the three sets, of which CLU was a critical factor for the development of HDP (OR = 1.22, P < 0.001). When these three factors and body weight were combined, the AUC was 0.74, with a sensitivity of 0.67 and specificity of 0.68 for HDP prediction compared with normal pregnancy. In addition, inflammation-induced CLU could inhibit the invasion of TEV-1 cells.

Conclusions: Complement proteins may play an essential role in the occurrence of HDP by acting on trophoblast cells. CLU may be a high-risk factor for HDP, and the models combining candidates show reasonable screening efficiency of HDP in the first half of pregnancy.
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http://dx.doi.org/10.1186/s12958-021-00742-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054419PMC
April 2021

Gut Microbiota Signatures in Gestational Anemia.

Front Cell Infect Microbiol 2021 25;11:549678. Epub 2021 Feb 25.

Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, China.

Gestational diseases are associated with altered intestinal microbiota in pregnant women. Characterizing the gut microbiota of gestational anemia (GA) may describe a novel role of gut microbial abnormality in GA. In this study, we investigated differences in gut microbiota between GA patients and healthy pregnant women from the first trimester (n = 24 54) and the third trimester (n = 30 56) based on the 16S rRNA gene sequencing method. No statistically significant differences in α-diversity were identified between GA patients and controls in the first trimester of pregnancy, whereas the Shannon index and observed OTUs were significantly lower in GA patients than in healthy controls in the third trimester. Distance-based redundancy analysis revealed striking differences in microbial communities in the third trimester between GA patients and controls. Four genera were significantly different in relative abundance between GA patients and healthy controls, while 12 genera differentiated significantly between GA patients and healthy controls in the third trimester. At the operational taxonomic unit (OTU) level, 17 OTUs and 30 OTUs were identified to be different between GA patients and healthy controls in the first and third trimesters, respectively. Changes in gut microbial composition of GA patients suggest a potential relation with GA, and provide insights into the prediction and intervention of gestational anemia.
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http://dx.doi.org/10.3389/fcimb.2021.549678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947918PMC
June 2021

Analysis of clinical characteristics of severe pertussis in infants and children: a retrospective study.

BMC Pediatr 2021 02 5;21(1):65. Epub 2021 Feb 5.

Department of Pediatrics, Beijing Ditan Hospital, Capital Medical University, Beijing, China.

Background: The incidence of pertussis shows an increasing trend in recent years, but some clinicians often lack sufficient understanding of the clinical characteristics and risk factors for severe pertussis, and more effective measures should be taken to reduce the incidence and mortality of pertussis in young infants METHODS: A retrospective study was conducted, and 184 infants and children with pertussis who had been hospitalized in the Department of Pediatrics of Beijing Ditan Hospital affiliated with Capital Medical University from January 2016 to December 2017 were included. Clinical data of the patients were collected and the clinical characteristics were statistically analyzed RESULTS: Among the 184 patients, 41.85% were infants < 3 months of age, and 65.22% of the total patients were not vaccinated against pertussis. There were 22 critically ill children, among whom 4 died, and compared with mild cases, they had a higher proportion of children younger than 3 months of age and infants not vaccinated against pertussis (63.64% vs. 38.89% and 100% vs. 60.49%, respectively); a higher proportion of children with severe pneumonia (100% vs. 0%); higher leukocyte count(× 109/L , 35.80 ± 20.53 vs 19.41 ± 8.59); and a higher proportion of children with severe hyperleukocytosis (18.18% vs. 0%, respectively) (P<0.05) CONCLUSIONS: 1. Infants aged <3 months not vaccinated for pertussis appear more likely to become infected and have more severe disease. 2. Severe pneumonia and hyperleukocytosis are the main mechanisms underlying severe pertussis.
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http://dx.doi.org/10.1186/s12887-021-02507-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863367PMC
February 2021

Epidemiological and clinical characteristics of 35 children with COVID-19 in Beijing, China.

Pediatr Investig 2020 Dec 28;4(4):230-235. Epub 2020 Dec 28.

Department of Pediatrics Beijing Ditan Hospital Capital Medical University Beijing China.

Importance: Within the coronavirus disease 2019 (COVID-19) global pandemic, more attention is warranted for whether this new infectious disease has unique manifestations in children.

Objective: To retrospectively determine the epidemiological and clinical characteristics of 35 children with COVID-19 in Beijing, China.

Methods: We collected data for 35 children diagnosed with COVID-19 who were admitted to Beijing Ditan Hospital from January 2020 to June 2020, and analyzed their epidemiological characteristics, clinical manifestations, laboratory examinations, chest imaging findings, treatments, and outcomes.

Results: The children comprised 18 boys (51.4%) and 17 girls (48.6%) aged 6 months to 15 years. All patients had clear epidemiological history, with family clusters accounting for 28 cases (80.0%) and clear tracing of exposure to high epidemic areas in the remaining 7 cases (20.0%). Four (11.4%) patients were classified as asymptomatic, 17 (48.6%) as acute upper respiratory infection, and 14 (40.0%) as mild pneumonia, with no severe or critical cases. Clinical manifestations were mild, including fever in 18 (51.4%), cough in 14 (40.0%), and nausea and diarrhea in 7 (20.0%) patients. White blood cell count was mostly normal (26 cases, 74.3%) or decreased (7 cases, 20.0%); lymphocyte percentage was increased in 24 (68.7%); neutrophil percentage was decreased in 25 (71.4%); alanine aminotransferase was increased in 3 (8.6%); and serum potassium was decreased in 4 (11.4%). Time to negative viral nucleic acid testing was 2-42 days (mean: 14.0 ± 9.4 days). Chest imaging examination revealed that 20 patients (57.1%) had different forms of lung inflammation. Treatment was mainly isolation and nutritional support. Eleven patients were treated with interferon atomization inhalation. No patients required oxygen therapy. All 35 children were cured and discharged. Length of hospital stay was 9-54 days (mean: 25.4 ± 13.8 days). During regular follow-up after discharge, 5 children showed positivity again in the viral nucleic acid test and were re-hospitalized for observation and treatment. The mean length of re-hospitalization stay was 10.8 days.

Interpretation: Children with COVID-19 mainly become infected within their family, and children of all ages are generally susceptible. The disease in children is mostly mild and the prognosis is good. Nucleic acid tests in some patients become positive again after discharge, suggesting that it is of great significance to carry out centralized isolation medical observations and active nucleic acid tests in close contacts for early detection of patients and routine epidemic prevention and control.
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http://dx.doi.org/10.1002/ped4.12230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768299PMC
December 2020

Differential expression of serum proteins before 20 weeks gestation in women with hypertensive disorders of pregnancy: A potential role for SH3BGRL3.

Placenta 2021 01 14;104:20-30. Epub 2020 Nov 14.

Department of Clinical Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, PR China. Electronic address:

Introduction: The objective of this study was to explore serum levels of differentially abundant proteins between women with hypertensive disorders of pregnancy (HDP) and women with normal-term pregnancy, and to explore the contribution of SH3BGRL3 to the pathogenesis of HDP.

Methods: At 6-20 weeks gestation 48 pregnant women who later developed HDP (HDP group) and 48 women with normal-term pregnancy (normal group) were recruited based on maternal age and gestational age at a 1:1 ratio. Total serum protein was extracted, denatured, deoxidized, and subjected to enzymolysis. The sample was labeled with Tandem Mass Tags and analyzed via mass spectroscopy to identify differentially abundant proteins. The role of SH3BGRL3 in trophoblast invasion, proliferation and apoptosis was examined using the HTR-8/SVneo cell line and primary isolates of extravillous trophoblast (EVT) cells.

Results: In the proteomic profiling analysis, there were 19 proteins that showed significant differential abundance (P < 0.05). Among them, 13 proteins were more abundant and 6 proteins were less abundant in the serum from the HDP group compared with the normal group. The function of one of the more abundant proteins, SH3BGRL3, in trophoblast cell invasion, proliferation and apoptosis was investigated. Treatment of the EVT cells or the HTR-8/SVneo cell line with anti-SH3BGRL3 inhibited proliferation, but stimulated both apoptosis and invasion. MMP2 and p-ERK levels were also decreased in EVT after anti-SH3BGRL3 treatment.

Discussion: The SH3BGRL3 protein can regulate various aspects of trophoblast biology, and may be useful in the clinical diagnosis of HDP.
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http://dx.doi.org/10.1016/j.placenta.2020.10.031DOI Listing
January 2021

Circulating expression of Hsa_circRNA_102893 contributes to early gestational diabetes mellitus detection.

Sci Rep 2020 11 4;10(1):19046. Epub 2020 Nov 4.

Department of Preventive Medicine, School of Public Health, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China.

Due to a poor availability of reliable biomarkers, detecting gestational diabetes mellitus (GDM) in early pregnancy remains a challenge. Novel biomarkers like Circular RNAs (circRNAs) may be a promising diagnostic tool. The aim of this study was (a) to identify circRNAs deregulated in GDM and (b) evaluate the potential of circRNAs in detecting GDM. The circRNAs expression profiling in 6 paired women (with and without GDM) was measured by microarray. The levels of five most relevant circRNAs were validated in 12 paired participants by qRT-PCR. To verify the reproducibility of qRT-PCR, significantly differential expressed circRNA levels were confirmed in 18 paired participants. A receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value. The areas under ROC curves of hsa_circRNA_102893 were 0.806 (95% CI 0.594-0.937) and 0.741 (0.568-0.872) in training set and test set, respectively. Circulating circRNAs reflect the presence of GDM. Hsa_circRNA_102893 may be a potential novel and stable noninvasive biomarker for detecting GDM in early pregnancy.
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http://dx.doi.org/10.1038/s41598-020-76013-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642424PMC
November 2020

circCRAMP1L is a novel biomarker of preeclampsia risk and may play a role in preeclampsia pathogenesis via regulation of the MSP/RON axis in trophoblasts.

BMC Pregnancy Childbirth 2020 Oct 27;20(1):652. Epub 2020 Oct 27.

Department of Clinical Laboratory, Shenzhen Longhua District Central Hospital, Guangdong Medical University, Shenzhen, 518110, Guangdong, China.

Background: Preeclampsia is a severe disease in pregnant women, which is primarily managed by early screening and prevention. Circular RNAs (circRNAs) have increasingly been shown to be important biological regulators involved in numerous diseases. Further, increasing evidence has demonstrated that circRNAs can be used as diagnostic biomarkers. This study was conducted to evaluate the potential of circCRAMP1L, previously identified to be downregulated in preeclampsia, as a novel biomarker for predicting the development of preeclampsia.

Methods: We measured the expression of circCRAMP1L, which is reportedly relevant to trophoblast physiology, in plasma samples from 64 patients with preeclampsia and 64 age-, gestational age-, and body mass index-matched healthy pregnant women by qRT-PCR. MTT proliferation and transwell invasion assays revealed the biological role of circCRAMP1L in preeclampsia pathogenesis. RNA immunoprecipitation and dual-luciferase reporter assays clarified the mechanism underlying the biological function of circCRAMP1L in TEV-1 cells.

Results: circCRAMP1L circulating levels were significantly lower in patients with preeclampsia (2.66 ± 0.82, △Ct value) than in healthy pregnant women (3.95 ± 0.67, △Ct value, p <  0.001). The area under the receiver operating characteristic curve for circCRAMP1L was 0.813. Univariate and multivariate analyses identified circCRAMP1L as an independent predictor of preeclampsia. Furthermore, when circCRAMP1L was utilised in combination with its target protein macrophage stimulating protein (MSP), the predictive performance increased, with an area under the receiver operating characteristic curve of 0.928 (95% CI 0.882-0.974), 80.0% sensitivity, and 80.0% specificity. The in vitro results indicated that circCRAMP1L regulates cell proliferation, and invasion via MSP and RON proteins. We investigated the molecular mechanisms of these effects. In vitro, relative to the control group, circCRAMP1L overexpression significantly enhanced cell proliferation; furthermore, trophoblast cell invasion increased proportionally with circCRAMP1L expression. RNA immunoprecipitation and luciferase reporter gene illustrated that circCRAMP1L participated in regulation of trophoblast cell by regulating MSP.

Conclusion: Reduced plasma levels of circCRAMP1L may be associated with an increased risk of preeclampsia, and circCRAMP1L may be a novel biomarker of preeclampsia risk.
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http://dx.doi.org/10.1186/s12884-020-03345-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590488PMC
October 2020

CircSFXN1 regulates the behaviour of trophoblasts and likely mediates preeclampsia.

Placenta 2020 11 12;101:115-123. Epub 2020 Sep 12.

Department of Clinical Laboratory, Shenzhen Longhua District Central Hospital, Guangdong Medical University, Shenzhen, Guangdong, 518110, China.

Preeclampsia (PE) is the second most common complication that threatens the health of pregnant women and their foetuses; however, the underlying mechanisms are poorly understood. Circular RNAs (circRNAs) are involved in various human diseases, and an increasing number of studies have revealed the vital role of circRNAs in PE. Here, we investigated the biological function of circRNA-SFXN1 (CircSFXN1) in PE and the associated molecular mechanisms. Microarray data analysis revealed that CircSFXN1 was highly expressed in PE placentas compared to control placentas; this finding was confirmed by qRT-PCR. In vitro, CircSFXN1 overexpression significantly inhibited the invasion of TEV-1 trophoblasts and blocked the angiogenesis of human umbilical vein endothelial cells (HUVECs), while CircSFXN1 knockdown promoted trophoblast invasion and stimulated HUVEC angiogenesis. For in vivo evaluation, pregnant Sprague-Dawley rats were randomly selected for tail vein injection with sFLT1-expressing adenovirus, which resulted in elevated blood pressure and increased proteinuria; si-CircSFXN1 reversed these increases. Mechanistic analyses via RNA-protein pulldown, RNA immunoprecipitation (RIP) and dual-luciferase reporter assays showed that CircSFXN1 recruits sFLT1 and modulates the biological behaviour of trophoblasts by binding sFLT1. In summary, we identified a novel circRNA that regulates tumorigenic activities, suggesting a new pathway governing CircSFXN1/sFLT1-mediated regulation of trophoblast behaviour.
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http://dx.doi.org/10.1016/j.placenta.2020.09.012DOI Listing
November 2020

Systematic analysis of gut microbiota in pregnant women and its correlations with individual heterogeneity.

NPJ Biofilms Microbiomes 2020 09 11;6(1):32. Epub 2020 Sep 11.

School of Public Health, Guangzhou Medical University, 511436, Guangzhou, China.

The woman's gut microbiota during pregnancy may support nutrient acquisition, is associated with diseases, and has been linked to infant health. However, there is limited information on gut microbial characteristics and dependence in pregnant women. In this study, we provide a comprehensive overview of the gut microbial characteristics of 1479 pregnant women using 16S rRNA gene sequencing of fecal samples. We identify a core microbiota of pregnant women, which displays a similar overall structure to that of age-matched nonpregnant women. Our data show that the gestational age-associated variation in the gut microbiota, from the ninth week of gestation to antepartum, is relatively limited. Building upon rich metadata, we reveal a set of exogenous and intrinsic host factors that are highly correlated with the variation in gut microbial community composition and function. These microbiota covariates are concentrated in basic host properties (e.g., age and residency status) and blood clinical parameters, suggesting that individual heterogeneity is the major force shaping the gut microbiome during pregnancy. Moreover, we identify microbial and functional markers that are associated with age, pre-pregnancy body mass index, residency status, and pre-pregnancy and gestational diseases. The gut microbiota during pregnancy is also different between women with high or low gestational weight gain. Our study demonstrates the structure, gestational age-associated variation, and associations with host factors of the gut microbiota during pregnancy and strengthens the understanding of microbe-host interactions. The results from this study offer new materials and prospects for gut microbiome research in clinical and diagnostic fields.
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http://dx.doi.org/10.1038/s41522-020-00142-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7486914PMC
September 2020

Prevalence, Characterization, and Drug Resistance of in Feces From Pediatric Patients in Guangzhou, China.

Front Med (Lausanne) 2020 24;7:127. Epub 2020 Apr 24.

Clinical Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

() is a major pathogen of human infections. Its fecal carriage serves as a risk factor for nosocomial transmission and disease development. However, the rate of fecal carriage among Chinese children has not yet been reported. Therefore, we sought to investigate the prevalence, characterization, and drug resistance of isolated from pediatric patients' feces in Southern China. Fecal samples (2059) from pediatric patients in three centers in Guangzhou were cultured. From which, 412 isolates were identified via selective mediums and automated VITEK Mass Spectrometer analysis. Antibiotic susceptibility was determined and DNA sequencing of seven housekeeping genes were used for multilocus sequence typing analysis. The fecal carriage rates were 20.0% for and 4.5% for methicillin-resistant (MRSA). Moreover, fecal carriage was positively correlated with outpatient status and gastroenteritis diagnosis. Moreover, age-related patterns were observed with respect to prevalence of . Besides, a total of 76 sequence types (STs) were identified, including 25 newly assigned STs and 28 clonal complexes (CCs). ST188, ST6, and ST15 were the most prevalent methicillin-sensitive (MSSA) clones, while ST59 and ST45 were the major MRSA clones. isolates also exhibited high rates of penicillin (84.2%), erythromycin (38.8%), and clindamycin (35.9%) resistance. Specifically, all ST30 and ST338 isolates were resistant to erythromycin and clindamycin, 61% of ST7 were resistant to tetracycline, and 84% of ST45 exhibited resistance and intermediate resistance to rifampicin. Also, CC59 (ST338 and ST59) and CC45 exhibited different antibiotic resistance patterns. These results demonstrate the colonization dynamics and molecular epidemiology of in child feces in Southern China. Further, they suggest an urgency for strengthening the surveillance programs in China and provide important information for the prevention and treatment of infection.
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http://dx.doi.org/10.3389/fmed.2020.00127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193981PMC
April 2020

Efficacy and safety of direct-acting antiviral-based treatment in hepatitis C virus infected patients with chronic renal function impairment: An updated systemic review and meta-analysis.

Nephrology (Carlton) 2020 Nov 12;25(11):829-838. Epub 2020 Mar 12.

Nephrology Department, Sichuan Provincial People's Hospital, Medical school of University of Electronic Science and Technology of China, Chengdu, China.

Objective: To further determine the efficacy and safety of direct-acting antiviral (DAA)-based treatments in hepatitis C virus (HCV) infected patients with renal function impairment.

Methods: MEDLINE, EMBASE and the Cochrane Library were searched for relevant studies. All studies assessing the efficacy and safety of DAA-based treatments against HCV infection in patients with renal impairment and HCV infection were eligible for inclusion. Outcomes assessed included efficacy outcomes and safety outcomes. Summary estimates were obtained using an inverse-variance weighted random effect model and Freeman-Tukey double arcsine transformation.

Results: Twenty-seven studies (n = 1048 participants) were included. The majority of included studies were of fair quality with Newcastle-Ottawa scale scores between 4 and 6. The pooled virologic response rates at the end of treatment or 4, 12, 24 weeks after treatment (ie, EOTR, SVR4, SVR12 and SVR24 rates) were 97.0% (95% confidence interval [CI], 94.0%-99.0%), 80.9% (95% CI, 49.3%-98.7%), 94.1% (95% CI, 91.6%-96.3%) and 89.6% (95% CI, 75.5%-98.1%), respectively. The pooled relapse rate was 6.4% (95% CI, 3.4%-10.4%). The pooled incidence of adverse events and severe adverse events leading to discontinuation were 47.6% (95% CI, 35.0%-60.4%) and 2.9% (95% CI, 1.4%-5.0%), respectively. High heterogeneity among studies exists for SVR4 and SVR24 rates. Formal statistical testing did not identify the presence of publication bias for all measured outcomes except the relapse rate.

Conclusion: The results support the efficacy and safety of DAA-based treatments in this population. Future studies with better design, larger sample size and longer follow up will be the next step. SUMMARY AT A GLANCE This systematic review evaluated the efficacy and safety of direct-acting antiviral based therapies in hepatitis C infection in patients with renal impairment. The majority of studies were of fair quality only. These therapies were found to be highly efficacious although there were high rates of adverse events.
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http://dx.doi.org/10.1111/nep.13704DOI Listing
November 2020

The Trickle-Down Effect of Leaders' Pro-social Rule Breaking: Joint Moderating Role of Empowering Leadership and Courage.

Front Psychol 2018 7;9:2647. Epub 2019 Jan 7.

School of Management, Guangdong Industry Polytechnic, Guangzhou, China.

Based on social learning theory and humanistic cognitive behaviorism theory, this study examined the trickle-down effect of leader PSRB and its boundary conditions. We proposed a three-way interaction of leader PSRB, empowering leadership, and follower courage to predict follower PSRB. Data were collected from 174 leader-follower dyads. Multiple moderated regressions (MMR) revealed that leader PSRB was positively related to follower PSRB, and that the effect was stronger under conditions of high empowering leadership or high courage. A three-way interaction effect suggested that the positive relationship between leader PSRB and follower PSRB was strongest when both empowering leadership and courage were high. Finally, the theoretical and practical implications were discussed.
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http://dx.doi.org/10.3389/fpsyg.2018.02647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330310PMC
January 2019

Prevalence and Characterization of Isolated From Women and Children in Guangzhou, China.

Front Microbiol 2018 16;9:2790. Epub 2018 Nov 16.

Clinical Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

The prevalent clones and antibiotic susceptibility profiles are known to change dynamically and geographically; however, recent strains causing infections in women and children in China have not been characterized. In this study, we analyzed the molecular epidemiology and antimicrobial resistance of isolated from patients in four centers for women and children in Guangzhou, China. In total, 131 isolates (100 from children and 31 from women) were analyzed by spa typing, multi-locus sequence typing, virulence gene and antimicrobial resistance profiling, staphylococcal chromosomal cassette typing, and mutation analyses of . A total of 58 spa types, 27 sequence types (STs), and 10 clonal complexes (CCs) were identified. While CC59 (ST59-IV, 48.8%; ST338-III, 35.7%) and CC45 (ST45-IV, 100%) were the major clones (84.4%) among MRSA isolates, CC5 (ST188, 24.3%; ST1, 21.6%) and CC398 (ST398, 70%) were the major ones (70.1%) among MSSA isolates. ST338-MRSA-III mostly found in pus but hardly in respiratory tract samples while ST45-MRSA-IV was on the opposite, even though they both found in blood and cerebrospinal fluid sample frequently. Staphylococcal enterotoxin genes -- were strongly associated with ST59 and ST338, while was associated with ST45, ST121, ST22, and ST30. All ST338, ST1232, and SCC III isolates carried genes. A total of 80% of ST338 isolates were resistant to erythromycin, clindamycin, and tetracycline. All ST45 isolates exhibited intermediate or complete resistance to rifampicin. In total, 481 HIS/ASN mutations in were found in rifampicin-resistant or intermediate-resistant isolates. ST338-III and ST45-IV emerged as two of three major clones in MRSA isolates from women and children in Guangzhou, China, though ST59-MRSA-IV remained the most prevalent MRSA clone. Clonal distribution of varied, depending on the specimen source. Virulence genes and antibiograms were closely associated with the clonal lineage. These results clarified the molecular epidemiology of from women and children in Guangzhou, China, and provide critical information for the control and treatment of infections.
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http://dx.doi.org/10.3389/fmicb.2018.02790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250813PMC
November 2018

Interfacial Force-Assisted In-Situ Fabrication of Graphene Oxide Membrane for Desalination.

ACS Appl Mater Interfaces 2018 Aug 6;10(32):27205-27214. Epub 2018 Aug 6.

Research Center for Environmental Material and Pollution Control Technology , University of Chinese Academy of Sciences , Beijing 100049 , People's Republic of China.

Graphene-based membranes have shown great potential application prospects in many fields, especially for water purification. Except for the current relatively low salt rejection rate, another main factor restricting application of such membranes is the lack of applicable preparation processes. In this work, a facile and cost-effective method was developed that can be used to in situ fabricate a graphene oxide (GO)-based membrane inside a filtration apparatus. Novel partial reduction and cross-linking was employed to adjust the surface properties and interlayer distance of GO membranes at the subnanometer range. A simple compacting process was applied to promote the integrity and compactness of the GO-based membranes by making full use of the interfacial tensions of gas/liquid/solid, which enables the in-situ fabrication. The as-prepared PrGO membranes show good water permeability (17.2-86.5 L m h MPa), reasonable desalination rates (27.7-62.6% for NaCl and 68.4-86.1% for NaSO), and good rejection rates of 92.3-96.8% for methyl orange. The method is appropriate for large-scale preparation and is theoretically not restricted by the shape or texture of the basement membrane, which represents another step forward in the fabrication of GO-based membranes toward wide-ranging applications.
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http://dx.doi.org/10.1021/acsami.8b08531DOI Listing
August 2018

CircRNA-0004904, CircRNA-0001855, and PAPP-A: Potential Novel Biomarkers for the Prediction of Preeclampsia.

Cell Physiol Biochem 2018 7;46(6):2576-2586. Epub 2018 May 7.

Department of Clinical Laboratory, Guangzhou Medical University Affiliated Guangzhou Women and Children's Medical Center, Guangzhou, China.

Background/aims: Circular RNAs (circRNAs) are transcribed prevalently in the genome; however, their potential roles in multiple cardiovascular diseases, particularly preeclampsia (PE), are not yet well understood. This study investigated the expression profiles of circRNAs and explored circRNA-mediated pregnancy-associated plasma protein A (PAPP-A) expression as a potential biomarker for PE before 20 weeks of pregnancy.

Methods: A nested case-control two-phase screening/validation study was performed in pregnant women before 20 weeks of gestation (before clinical diagnosis) at Guangzhou Women and Children's Medical Center from 2012 to 2015. In the screening phase, circRNA expression profiles of blood cells were assessed using a human circRNA microarray, which was designed to detect simultaneously 5396 circRNAs, in 5 patients with PE and 5 age- and gestational week-matched controls. In the validation phase, 18 circRNAs in blood cells predicted by bioinformatics tools were validated by quantitative reverse transcription PCR in a cohort of 60 patients (PE and age-, gestational week-, and sample storage time-matched controls). Then, we examined the involvement of circRNAs in PE-related pathways via interactions with miRNAs by multiple bioinformatics approaches. Bioinformatics analysis predicted that hsa_circ_0004904 and hsa_circ_0001855 miRNA sponges directly target PAPP-A. PAPP-A was verified in the serum of the same cohort of patients using an enzyme-linked immunosorbent assay. Finally, we combined PAPP-A with circRNAs to create a novel preclinical diagnostic model for PE with logistic regression and evaluated the efficiency of this model with receiver operating curve analysis.

Results: Volcano plot analysis using various parameters showed that circRNAs were differentially expressed among both groups (P < 0.01, fold change > 2). In the screening phase, we found that 2178 circRNAs were differentially expressed between the control and PE groups, in which 884 circRNAs were downregulated and 1294 circRNAs were upregulated in the PE group compared with the control group. In the validation phase, two circRNAs, hsa_circ_0004904 and hsa_circ_0001855, were significantly upregulated in PE patients compared with healthy pregnant women (P < 0.05). PAPP-A expression levels, related to the two circRNAs based on bioinformatics prediction, were increased in the PE group compared with the control group. The area under the curve of the combined model was 0.94 in the predicted PE subjects.

Conclusions: This is the first study to report circRNA profiling in patients with PE prior to the onset of symptoms. Our data suggested that hsa_circ_0004904 and hsa_circ_0001855 combined with PAPP-A might be promising biomarkers for the detection of PE. Moreover, circRNAs may provide new insights into the potential mechanisms underlying the pathophysiology of PE.
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http://dx.doi.org/10.1159/000489685DOI Listing
August 2018

Unique manifestations and risk factors of Jarisch-Herxheimer reaction during treatment of child congenital syphilis.

Sex Transm Infect 2018 12 23;94(8):562-564. Epub 2017 Sep 23.

Department of Paediatrics, Beijing Ditan Hospital, Capital Medical University, Beijing, China.

Objectives: The objective of this retrospective study was to summarise the clinical manifestations of, and to analyse the incidence and risk factors of, Jarisch-Herxheimer reaction (JHR) during the treatment of children with symptomatic congenital syphilis.

Methods: Clinical data of 60 children with clinically and laboratory diagnosed congenital syphilis, hospitalised in Beijing Ditan Hospital between January 2010 and November 2015, were collected and analysed.

Results: A total of 11 patients with congenital syphilis (11/60, 18.3%) developed JHR. JHR occurred in 1-6 hour after the first dose of penicillin. Common clinical manifestations included fever (11/11, 100%), irritability (11/11, 100%), rapid pulse and breathing (11/11, 100%), exacerbation of existing rash (5/11, 45.6%) and chills (3/11, 27.3%). Of the 11 patients who developed JHR, 9 patients (9/11, 81.8%) had bone syphilis, 10 (10/11, 90.9%) had more than three organs affected by syphilis and 10 (10/11, 90.9%) had a high plasma concentration of rapid plasma reagin (RPR) (≥1:256); these percentages were significantly higher than in patients who had not developed JHR (p<0.05), suggesting that the occurrence of JHR was related to bone syphilis, having more than three organs affected by syphilis and a high plasma concentration of RPR.

Conclusions: Clinicians should be familiar with the risk factors for this reaction and its common clinical manifestations.
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http://dx.doi.org/10.1136/sextrans-2016-053083DOI Listing
December 2018

Insight into the HS selective catalytic oxidation performance on well-mixed Ce-containing rare earth catalysts derived from MgAlCe layered double hydroxides.

J Hazard Mater 2018 Jan 9;342:749-757. Epub 2017 Sep 9.

Center of Research & Development, Shandong Sunway Petrochemical Engineering Share Co., Ltd, Beijing 100015, PR China.

A series of well-mixed Ce-containing MgAlCe rare earth catalysts derived from layered double hydroxides were synthesized and tested for HS selective catalytic oxidation. Particularly, no chemisorption O-vacancies but intrinsic defect sites were present on catalyst surface. Significantly, the catalysts exhibited excellent catalytic activity, reasonable durability, and outstanding sulfur selectivity (100%) at relatively low temperatures. Furthermore, the catalyst followed a step-wise mechanism, and the catalyst deactivation was due mainly to the slower oxidation rate of Ce to Ce by O as compared to the reduction rate of Ce to Ce by HS. Particularly, the added water, a Lewis base, can compete with inefficient S catalyst for the occupation of Lewis acid sites and active sites. Meanwhile, it can change the characteristics of catalyst surface, resulting in sulfur existing form transforming from inefficient S catalyst to inactive S. Thus, lead to a decrease of deposited inefficient S catalyst content. Consequently, decrease the catalytic activity.
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http://dx.doi.org/10.1016/j.jhazmat.2017.09.014DOI Listing
January 2018

MiR-942 decreased before 20 weeks gestation in women with preeclampsia and was associated with the pathophysiology of preeclampsia in vitro.

Clin Exp Hypertens 2017 28;39(2):108-113. Epub 2017 Feb 28.

c Department of Clinical Laboratory , Xiangzhou District People's Hospital , Zhuhai , China.

Objective: To investigate the possible relationship between miR-942 levels and the pathogenesis of preeclampsia using in vitro assays and to investigate circulating miR-942 levels in the early phase of mid-of pregnancy in women who later developed preeclampsia and in women with uncomplicated pregnancies.

Methods: Plasma samples were collected from pregnant women between 12 and 20 weeks of gestation. MiR-942 levels were determined by stem-loop real-time PCR for 26 cases who subsequently developed preeclampsia as well as for 52 controls. Bioinformatics software was used to predict the target genes of miR-942, and a dual-luciferase reporter system was utilized to validate target gene regulation. Finally, MTT proliferation assays, transwell invasion assays, and endothelial cell tube formation assays were performed to further explore the function of miR-942 using a human extravillous trophoblast cell line (TEV-1).

Result: Circulating miR-942 levels were significantly lower in mid-pregnancy (12-20 weeks gestation) in women who later developed preeclampsia compared with those with an uncomplicated pregnancy (p < 0.05). Endoglin (ENG) is an miR-942 target gene. MiR-942 had a sensitivity of 0.673, a specificity of 0.875, and an area under the receiver operating characteristic curve (AUC) of 0.718 [95% CI, 0.594-0.822] for the possible screening of preeclampsia. In vitro, decreased miR-942 expression decreased the invasive ability of TEV-1 cells, and inhibited the HUVEC angiogenesis assay, both effects that are similar to what is observed in preeclampsia (both p <0.05).

Conclusion: MiR-942 may be involved in the pathogenesis of preeclampsia via the regulation of its target gene ENG. Multicenter studies must be performed and a greater number of samples must be analyzed to ascertain whether circulating miR-942 levels can serve as a novel early diagnostic marker for preeclampsia.
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http://dx.doi.org/10.1080/10641963.2016.1210619DOI Listing
June 2017

Efficacy and safety assessment of acupuncture and nimodipine to treat mild cognitive impairment after cerebral infarction: a randomized controlled trial.

BMC Complement Altern Med 2016 Sep 13;16:361. Epub 2016 Sep 13.

Department of Acupuncture, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.

Background: Cerebral infarction frequently leads to mild cognitive impairment (MCI). Prompt management of MCI can prevent vascular dementia and improve patient outcome. This single center randomized controlled trial aims to investigate the efficacy and safety of acupuncture and nimodipine to treat post-cerebral infarction MCI.

Methods: A total of 126 Chinese patients with post-cerebral infarction MCI recruited from the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine between April 2013 and June 2014 were randomized at 1:1: 1 ratio into nimodipine alone (30 mg/time and 3 times daily), acupuncture alone (30 min/time, 6 times/week), and nimodipine + acupuncture groups. The treatments were 3 months. Cognitive function was evaluated using Montreal Cognitive Assessment (MoCA) scale at enrollment interview, at the end of 3-month therapy, and at the post-treatment 3-month follow-up.

Results: The per-protocol set included 39, 40, and 40 patients from nimodipine alone, acupuncture alone, and the combination group, respectively, was analyzed. Intra-group comparison revealed that MoCA score at the follow-up improved significantly by 15.8 ± 10.9, 20.9 ± 13.8 %, and 30.2 ± 19.7 % compared with the baseline MoCA for nimodipine alone, acupuncture alone, and the combination group, respectively. Inter-group comparison demonstrated that the combination therapy improved MoCA score (5.5 ± 2.2) at significantly higher extent than nimodipine alone (3.1 ± 1.8) and acupuncture alone (4.3 ± 2.3) at the follow-up (All P < 0.05), and significantly higher proportion of patients in acupuncture alone group (80 %) and the combination therapy group (90 %) than in nimodipine alone group (56.4 %) showed ≥12 % MoCA score improvement compared with the baseline MoCA (All P < 0.05). No adverse event was reported during the study.

Conclusion: Acupuncture may be used as an additional therapy to conventional pharmacological treatment to further improve the clinical outcomes of patients with post-cerebral infarction MCI.

Trial Registration: The study was registered at the Chinese Clinical Trial Registry ( http://www.chictr.org.cn/ , Unique Identifier: ChiCTR-IOR-15007366 ). The date of registration is November 4, 2015.
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http://dx.doi.org/10.1186/s12906-016-1337-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022140PMC
September 2016

Plasma Level of Placenta-Derived Macrophage-Stimulating Protein -Chain in Preeclampsia before 20 Weeks of Pregnancy.

PLoS One 2016 25;11(8):e0161626. Epub 2016 Aug 25.

Department of Preventive Medicine, School of Public Health, Guangzhou Medical University, Guangzhou, 511436, China.

Object: This study aimed to investigate the diagnostic value of placenta-derived macrophage-stimulating protein α-chain (MSP-α) before the 20th week of gestation for the early diagnosis of preeclampsia (PE).

Methods And Materials: Two parts of this nested case-control study were simultaneously executed, and 1500 pregnant women were recruited. A total of 124 pregnant women were included in the plasma analysis part of this study. The MSP-α plasma level was measured before the 20th week of gestation, and the participants were followed until delivery. A case group of 62 women with PE and a control group of 62 women matched by gestational age, maternal age, and pre-pregnancy BMI (with normotensive pregnancies) were evaluated. In the placenta analysis part of this nested case-control study, the placentas of 34 pregnant women were randomly obtained. The placental levels of MSP were measured in 17 individuals with PE (case group) and in 17 women with a normotensive pregnancy matched by gestational age and maternal age (control group).

Results: The plasma level of MSP-α was higher in the PE group than in the control group before the 20th week of gestation (p < 0.001). In addition, compared to the women with severe features in the PE group, those without severe features had a significantly higher plasma MSP-α level before the 20th week of gestation (p < 0.001). The area under the receiver operating characteristic curve (AUC) of MSP-α before the 20th week of gestation was 0.905 (95% CI, 0.811-0.962) for the women with early-onset PE without severe features. With regard to the placenta, the PE group (accumulated optical density, IOD [SUM] = 8862.37 ± 2064.42) exhibited increased MSP staining (more intense MSP staining or more extensive staining) compared with the control group (normal pregnancies (IOD [SUM] = 447.92 ± 114.72, P < 0.001). Furthermore, increased MSP staining was detected among the women without severe features compared with those with severe features in the PE group (IOD [SUM]: 12192.65 ± 5325.56 vs. 4104.83 ± 2383.06, P = 0.021).

Conclusion: According to the findings of this study, the plasma level of MSP-α may be associated with PE, and MSP-α may be considered a candidate protein for further analysis in studies of PE. Multicenter studies with larger sample sizes must be performed in the future to obtain accurate results regarding the predictive value of MSP-α in combination with other protein factors for the early diagnosis of PE.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161626PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999075PMC
August 2017

Both pelvic radiography and lateral abdominal radiography correlate well with coronary artery calcification measured by computed tomography in hemodialysis patients: A cross-sectional study.

Hemodial Int 2016 07 1;20(3):399-406. Epub 2016 Mar 1.

Division of Nephrology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.

Introduction Lateral abdominal radiograph is suggested as an alternative to coronary artery computed tomography (CT) in evaluating vascular calcification. Simple scoring systems including pelvic radiograph scoring and abdominal scoring system were utilized to study their correlation with coronary artery calcification. Methods In 106 MHD patients, coronary artery CT, lateral abdominal, and pelvic radiograph were taken. The Agatston scoring system was applied to evaluate the degree of coronary artery calcification which was categorized according to Agatston coronary artery calcification score (CACS) ≥ 30, ≥100, ≥400, and ≥1000. Abdominal aortic calcification was scored by 4-scored and 24-scored systems. Pelvic artery calcification was scored by a 4-scored system. Sensitivities and specificities of abdominal aortic calcification scores and pelvic artery calcification scores to predict different categories of coronary artery calcification were analyzed. We studied the diagnostic capability of abdominal aorta calcification and pelvic artery calcification to predict different CACS categories by calculating likelihood ratios. Receiver operator characteristic curves were used to determine the area under the curve for each of these testing procedures. Findings The prevalence was 48(45.3%), 15 (14.2%), 11 (10.4%), 11 (10.4%), and 11 (10.4%) for CACs > 0, ≥30, ≥100, ≥400, and ≥1000, respectively. The degree of CACs was positively correlated with patient age, prevalence of diabetes, abdominal aorta scores, and pelvic calcification scores. The areas under the curves for different CACS by all X-ray scoring systems were above 0.70 except pelvic 4-scored system for diagnosing CACS ≥30, without significant difference (P > 0.05). Discussion Both lateral abdominal and pelvic plain radiographs were demonstrated as acceptable alternatives to CT in evaluating vascular calcification.
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http://dx.doi.org/10.1111/hdi.12399DOI Listing
July 2016

Self-rated chronic conditions and 2-week prevalence in adults in Zhongshan, China: an epidemiological survey.

BMJ Open 2015 Nov 11;5(11):e008441. Epub 2015 Nov 11.

Department of Internal Medicine, Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, North Carolina, USA.

Objective: To examine the association between behavioural factors and the risk of chronic conditions and 2-week prevalence.

Design: This was a cross-sectional survey.

Setting: The study was conducted in Zhongshan, China.

Participants: A multistage clustering sampling method was used to select a representative sample of residents from the household registration system between July and September 2011. The overall sample replacement rate was 9.4%, and the final sample included 43 028 individuals.

Outcome Measures: Chronic conditions and 2-week prevalence.

Results: 4979 (11.6%) of the participants reported having at least one chronic condition, 1067 (2.5%) had two or more concurrent chronic conditions, and 6830 (15.9%) reported having at least one disease in a 2-week recall period. The most common chronic condition was primary hypertension, which was reported by 6.8% of participants. Logistic regression models demonstrated that the main factors for having a chronic condition and 2-week prevalence were older age (≥65 years of age; OR 44.91, 95% CI 33.05 to 61.03; and OR 12.71, 95% CI 10.44 to 15.46, respectively), obesity (OR 3.00, 95% CI 2.63 to 3.42; and OR 2.50, 95% CI 2.22 to 2.82, respectively) and being a former smoker (OR 3.02, 95% CI 2.54 to 3.58; and OR 3.24, 95% CI 2.74 to 3.82, respectively).

Conclusions: This study suggests that older age, obesity and unhealthy behaviours are high-risk factors for poorer health status among the residents of Zhongshan, China. The present findings highlight the importance of recognising and managing harmful behaviours in order to improve health.
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http://dx.doi.org/10.1136/bmjopen-2015-008441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4654384PMC
November 2015
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