Publications by authors named "Hongli Wang"

373 Publications

The clinical characteristics of Hirayama disease in females.

Neurologia (Engl Ed) 2022 Aug 10. Epub 2022 Aug 10.

Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China; Spine Center Fudan University, Shanghai, 200040, China. Electronic address:

Introduction: To characterize Hirayama disease in female patients, and increase awareness among clinicians regarding the specifics of this disease.

Methods: Baseline data, clinical manifestations, characteristics of cervical-flexion magnetic resonance imaging, and electromyography were collected and compared among females and males with Hirayama disease. In addition, the literature on Hirayama disease in females up to October, 2021 was searched in PubMed and the relevant data were compared with the data from our study.

Results: Twenty female and 40 male patients were included in this study. The average ages of onset and menarche were 14.65 and 12.75 years old. All patients suffered from muscular weakness and atrophy of the upper limb(s), with flattening and/or atrophy of the lower cervical spinal cords in cervical-flexion magnetic resonance imaging, and neurogenic patterns in the atrophic muscles as determined using electromyography. The age of onset in females was about 2 years later than the age of menarche, and the age of onset in females was 2 years earlier than that in males. There were no obvious differences in clinical presentation between males and females.

Discussion: Although females presented with Hirayama disease two years earlier than males, no other clinical differences were observed. Hirayama disease is likely associated with growth and development in puberty, and early identification, regardless of whether patients are male or female, is critical to optimizing prognosis.
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http://dx.doi.org/10.1016/j.nrleng.2022.06.004DOI Listing
August 2022

Circ_0003611 regulates apoptosis and oxidative stress injury of Alzheimer's disease via miR-383-5p/KIF1B axis.

Metab Brain Dis 2022 Aug 12. Epub 2022 Aug 12.

Department of Neurosurgery, Affiliated Hospital of Guilin Medical University, No.15 Lequn Road, Xiufeng District, Guilin, China.

Alzheimer's disease (AD) is a high incidence neurodegenerative disease. Emerging evidence suggests that circular RNAs (circRNAs) play an important modulator in the pathogenesis of AD. The aim of this paper was to reconnoiter the effects of circular RNA_0003611 (circ_0003611) on Aβ-triggered neuronal injury in AD. In this work, the abundance of circ_0003611 was augmented in AD patients and SH-SY5Y and SK-N-SH cells treated with Aβ. Aβ-mediated cell proliferation, apoptosis, inflammatory response, oxidative stress, and glycolysis were abolished through circ_0003611 silencing. Circ_0003611 worked as a miR-383-5p sponge, and the protective role of circ_0003611 absence on Aβ-triggered neuronal injury was overturned by releasing miR-383-5p. Meanwhile, miR-383-5p directly targeted KIF1B, and miR-383-5p upregulation might relieve Aβ-triggered neuronal injury by reducing KIF1B expression. Mechanical analysis discovered that circ_0003611 served as a sponge of miR-383-5p to impact KIF1B expression. These findings indicated that circ_0003611 improved Aβ-triggered neuronal injury in AD through targeting the miR-383-5p/KIF1B axis, which might deliver innovative therapy targeting for AD.
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http://dx.doi.org/10.1007/s11011-022-01051-zDOI Listing
August 2022

miR-34a induces neutrophil apoptosis by regulating Cdc42-WASP-Arp2/3 pathway-mediated F-actin remodeling and ROS production.

Redox Rep 2022 Dec;27(1):167-175

Department of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, People's Republic of China.

Background: The number of neutrophils is significantly reduced in myelodysplastic syndrome (MDS), but the molecular basis remains unclear. We recently found that miR-34a was significantly increased in MDS neutrophils. Therefore, this study aims to clarify the effects of aberrant miR-34a expression on neutrophil counts.

Methods: miR-34a mimics/inhibitor transfection were performed in neutrophil-like differentiated HL60 (dHL60) cells, and a FACSCalibur flow cytometer was used to measure ROS production and apoptosis. In addition, the Cdc42-WASP-Arp2/3 pathway inhibitor (ML141) and activator (CN02) treated the dHL60 cells, and then ROS production, apoptosis and related proteins expression were detected. And, luciferase reporter assay to verify the relationship of miR-34a and the Cdc42-WASP-Arp2/3 pathway.

Results: overexpression of miR-34a could induce ROS production and apoptosis, decrease the expression levels of DOCK8, p-WASP, WASP, Arp2, Arp3, and increase F-actin's expression. Meanwhile, knockdown of miR-34a could decrease ROS production and apoptosis, increase the expression of DOCK8, p-WASP, WASP, Arp2, Arp3, and decrease F-actin's expression. Immunofluorescence staining showed aberrant miR-34a and Cdc42-WASP-Arp2/3 pathway could induce F-actin membrane transfer. Luciferase reporter assay indicated that DOCK8 was a direct target gene of miR-34a.

Conclusion: These data indicates miR-34a may induce neutrophil apoptosis by regulating Cdc42-WASP-Arp2/3 pathway-mediated F-actin remodeling and ROS production.
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http://dx.doi.org/10.1080/13510002.2022.2102843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9364709PMC
December 2022

Fate of Oxygenated Volatile Organic Compounds in the Yangtze River Delta Region: Source Contributions and Impacts on the Atmospheric Oxidation Capacity.

Environ Sci Technol 2022 Aug 4;56(16):11212-11224. Epub 2022 Aug 4.

Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Nanjing University of Information Science & Technology, Nanjing 210044, China.

The Community Multiscale Air Quality model (CMAQv5.2) was implemented to investigate the sources and sinks of oxygenated volatile organic compounds (OVOCs) during a high O and high PM season in the Yangtze River Delta (YRD) region, based on constraints from observations. The model tends to overpredict non-oxygenated VOCs and underpredict OVOCs, which has been improved with adjusted emissions of all VOCs. The OVOCs in the YRD are dominated by ketones, aldehydes, and alcohols. Ketones and aldehydes mainly originate from direct emissions and secondary formation in the northern YRD, and primarily originate from secondary formation in the southern part influenced by biogenic emissions. The concentration of secondary organic aerosols (SOA) produced by OVOCs is 0.5-1.5 μg/m, with 40-80% originated from organic nitrates, 20-70% originated from dicarbonyls, and 0-20% originated from isoprene epoxydiols. The influences of OVOCs on the atmospheric oxidation capacity are dominated by the OH pathway during the day (∼350%) and by the NO pathway at night (∼150%). Consequently, O is enhanced by 30-70% in the YRD. Aerosols are also enhanced by 50-140%, 20-80%, and ∼20% for SOA, nitrate, and sulfate, respectively.
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http://dx.doi.org/10.1021/acs.est.2c00038DOI Listing
August 2022

Effect of oral administration of GABA on thermoregulation in athletes during exercise in cold environments: A preliminary study.

Front Nutr 2022 15;9:883571. Epub 2022 Jul 15.

College of Physical Education and Health, Guangxi Normal University, Guilin, China.

Background: γ-aminobutyric acid (GABA), a common ingredient in sports supplements and other health products, regulates body temperature in the preoptic area and anterior hypothalamus (PO/AH). To date, no study has examined the effect of GABA on thermoregulation during exercise in humans in a cold temperature environment (11 ± 0.3°C, 45% ± 2% relative humidity).

Methods: We performed a randomized, double-blind study. Ten trained male athletes consumed either a drink (3 ml/kg weight) containing GABA (1,000 mg, trial G) or an equivalent amount of placebo drink (trial C) before exercise. They rested for 20 min and then cycled at 60% of maximum output power for 40 min, pedaling at 60 rpm, and recovered for 20 min. Core temperature (T), skin temperature (upper arm, chest, thigh, calf), and heart rate (HR) were monitored at rest (T), exercise begins (T), 20 min of exercise (T), the exercise ends (T), and at recovery (T).

Results: Compared to T, T decreased significantly at T and increased significantly at T, T and T ( < 0.01). From 35-80 min, the T was higher in trial G (peaked at 37.96 ± 0.25°C) than in trial C (37.89 ± 0.37°C), but it failed to reach significant difference ( > 0.05); T continued to increase during exercise and was significantly higher than T at T ( < 0.05), T and T ( < 0.01). There was no significant difference in T between the two trials ( > 0.05).

Conclusion: Our findings provide initial evidence that oral administration of GABA does not affect thermoregulation and has no adverse effects on the body as an ergogenic exercise supplement during exercise in cold environments.
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http://dx.doi.org/10.3389/fnut.2022.883571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335056PMC
July 2022

Disulfide Metathesis-Assisted Lithium-Ion Conduction for PEO-Based Polymer Electrolytes.

ACS Macro Lett 2022 Aug 20;11(8):991-998. Epub 2022 Jul 20.

Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

The disulfide metathesis is a promising candidate in the dynamically exchanged strategy for improving the self-healing ability of polymer electrolytes (PEs). However, the enhancement effects on the ionic conductivities of PEs are generally ignored while introducing a dynamic covalent bond to PEs. Herein, the oligo(ethylene oxide)-based additive containing a disulfide bond (S-S additive) was synthesized via Michael addition reaction of cystamine and poly(ethylene glycol) methyl ether acrylate (PEGA). Short PEG chains complexed with Li in a S-S additive migrated rapidly in PEs because of the dynamically exchanged strategy of the disulfide bond. Moreover, disulfide bonds in a S-S additive possessed the ability to exchange with the cross-linked network containing disulfide bonds (S-S net). The as-prepared PEs exhibited a high room temperature ionic conductivity of 1.24 × 10 S cm, demonstrating that the disulfide metathesis-assisted Li conduction was feasible for enhancing ionic conductivities of PEs. Relative to other PEO-based PEs, these disulfide-containing PEs possessed a high Li transference number (0.54). Furthermore, the lithium-metal batteries (LMBs) assembled with PEs in the presence of a S-S additive presented stable cycle performance, indicating the promising potential of these PEs as candidates for next-generation LMBs.
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http://dx.doi.org/10.1021/acsmacrolett.2c00404DOI Listing
August 2022

An online method for monitoring atmospheric intermediate volatile organic compounds with a thermal desorption-gas chromatography/mass spectrometry.

J Chromatogr A 2022 Aug 5;1677:463299. Epub 2022 Jul 5.

State Environmental Protection Key Laboratory of Formation and Prevention of Urban Air Pollution Complex, Shanghai Academy of Environmental Sciences, Shanghai 200233, China.

As one of important precursors of secondary organic aerosol (SOA), intermediate volatile organic compounds (IVOCs) have attracted much attention in recent years. Most of the previous studies however largely focused on characteristics of IVOCs from different emission sources, while data from field observations to study their temporal variations was limited for lacking the sufficient time resolution monitoring data. In this study, an online thermal desorption-gas chromatography/mass spectrometry (TD-GC/MS) method was developed to generate monitor data with a three-hour time resolution for gaseous atmospheric IVOCs. The method used two multi-sorbent traps that alternated for conducting sample collection and sample analysis. Compounds of CC n-alkanes and 2-4 ring PAHs were chosen as surrogates to evaluate the performance of this method. Regression coefficients of external calibration curves were greater than 0.93 and 0.96 for all individual n-alkanes and PAHs, respectively. Average relative standard deviation (RSD) values among replicate samples spiked at 3 ng for each individual standard were 9% ± 5%. The detection limits of this method for individual n-alkanes and PAHs were 3.1-16.2 ng/m and 1.0-2.7 ng/m, respectively. Atmospheric IVOCs were continuously monitored from September 28 to 30 and October 22 to November 9 in 2018, in an urban area of Shanghai. Besides targeted n-alkanes and PAHs, unspeciated complex mixtures (UCM) of IVOCs as well as total-IVOCs concentrations in the atmosphere were also determined. Measured concentrations and compositions of gaseous IVOCs in the atmosphere in this study were comparable to other similar studies.
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http://dx.doi.org/10.1016/j.chroma.2022.463299DOI Listing
August 2022

Assessing heavy-duty vehicles (HDVs) on-road NO emission in China from on-board diagnostics (OBD) remote report data.

Sci Total Environ 2022 Jul 6:157209. Epub 2022 Jul 6.

State Environmental Protection Key Laboratory of Vehicle Emission Control and Simulation, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

Mobile source emissions are some of the major causes of air pollution across the world and are associated with numerous adverse health impacts. China has implemented increasingly stringent emission standards over the past few decades, the most recent one being the sixth emission standard (China VI) first rolled out in 2019. The China VI standard places special emphasis on tightening limits for NO emission and introduces remote monitoring of vehicles' On-board diagnostics (OBD) data to reduce emissions from diesel and gas-powered heavy-duty vehicles (HDV). This paper aims to establish a methodology to calculate HDV NO emissions on an extended timescale, and under real-world operations. OBD data was collected and examined from 53 China VI diesel HDVs and 14 China V diesel HDVs, which found that the average NO emission factors are 1.420 g/km and 3.894 g/km for the two samples respectively; this indicates that the implementation of China VI standard helps to significantly reduce NO emission from HDVs. Combining the emission factors and calculated travel distances collected by OBD and vehicle sales data, the China VI emission standard is estimated to have resulted in 43,969 tons of NO emission reduction by the end of 2020. With China announcing country-wide enforcement of the new standard in 2021, >1.7 million tons of NO emission could be avoided by 2023 annually.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157209DOI Listing
July 2022

Machine Learning-Based Gray-Level Co-Occurrence Matrix (GLCM) Models for Predicting the Depth of Myometrial Invasion in Patients with Stage I Endometrial Cancer.

Cancer Manag Res 2022 30;14:2143-2154. Epub 2022 Jun 30.

Department of Oncology, the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, 445000, People's Republic of China.

Purpose: Deep myometrial invasion (DMI) is an independent high-risk factor for lymph node metastasis and a prognostic risk factor in early-stage endometrial cancer (EC-I) patients. Thus, we developed a machine learning (ML) assistant model, which can accurately help define the surgical area.

Methods: 348 consecutive EC-I patients with the pathological diagnosis were recruited in the tertiary medical centre between January 1, 2012, and October 31, 2021. Five ML-assisted models were developed using two-step estimation methods from the candidate gray level co-occurrence matrix (GLCM). Receiver operating characteristic curve (ROC), decision curve analysis (DCA), and clinical impact curve (CIC) were prepared to evaluate the robustness and clinical practicality of each model.

Results: Our analysis identified several significant differences between the stage IA and IB groups. The top seven-candidate factors included correlation all direction offset1, correlation angle0 offset1, correlation angle45 offset1, correlation angle90 offset1, ID moment all direction offset1, ID moment angle0 offset1, and ID moment angle45 offset1. The areas under the ROC curve (AUCs) of the random forest classifier (RFC) model, support vector machine (SVM), eXtreme gradient boosting (XGBoost), artificial neural network (ANN), and decision tree (DT) ranged from 0.765 to 0.877 in the training set and from 0.716 to 0.862 in the testing set, respectively. Among the five machine algorithms, RFC obtained the optimal prediction efficiency using correlation angle0 offset1, correlation angle45 offset1, correlation angle90 offset1, correlation all direction offset1, ID moment angle0 offset1, and ID moment angle45 offset1, and ID moment angle90 offset1, respectively.

Conclusion: Our ML-based prediction model combined with GLCM parameters assessed the risk of DMI in EC-I patients, especially RFC, which helped distinguish stage IA and IB EC patients. This new predictive model based on supervised learning can be used to establish personalized treatment strategies.
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http://dx.doi.org/10.2147/CMAR.S370477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252192PMC
June 2022

Modeling particulate nitrate in China: Current findings and future directions.

Environ Int 2022 Jun 22;166:107369. Epub 2022 Jun 22.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; CAS Center for Excellence in Regional Atmospheric Environment, Chinese Academy of Science, Xiamen 361021, China. Electronic address:

Particulate nitrate (pNO) is now becoming the principal component of PM during severe winter haze episodes in many cities of China. To gain a comprehensive understanding of the key factors controlling pNO formation and driving its trends, we reviewed the recent pNO modeling studies which mainly focused on the formation mechanism and recent trends of pNO as well as its responses to emission controls in China. The results indicate that although recent chemical transport models (CTMs) can reasonably capture the spatial-temporal variations of pNO, model-observation biases still exist due to large uncertainties in the parameterization of dinitrogen pentoxide (NO) uptake and ammonia (NH) emissions, insufficient heterogeneous reaction mechanism, and the predicted low sulfate concentrations in current CTMs. The heterogeneous hydrolysis of NO dominates nocturnal pNO formation, however, the contribution to total pNO varies among studies, ranging from 21.0% to 51.6%. Moreover, the continuously increasing PM pNO fraction in recent years is mainly due to the decreased sulfur dioxide emissions, the enhanced atmospheric oxidation capacity (AOC), and the weakened nitrate deposition. Reducing NH emissions is found to be the most effective control strategy for mitigating pNO pollution in China. This review suggests that more field measurements are needed to constrain the parameterization of heterogeneous NO and nitrogen dioxide (NO) uptake. Future studies are also needed to quantify the relationships of pNO to AOC, O, NOx, and volatile organic compounds (VOCs) in different regions of China under different meteorological conditions. Research on multiple-pollutant control strategies involving NH, NO and VOCs is required to mitigate pNO pollution, especially during severe winter haze events.
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http://dx.doi.org/10.1016/j.envint.2022.107369DOI Listing
June 2022

Upgrading Emission Standards Inadvertently Increased OH Reactivity from Light-Duty Diesel Truck Exhaust in China: Evidence from Direct LP-LIF Measurement.

Environ Sci Technol 2022 07 30;56(14):9968-9977. Epub 2022 Jun 30.

Institute of Environmental and Climate Research, Jinan University, Guangzhou 510632, China.

Vehicular exhaust is an important source of reactive gases responsible for the formation of ozone and secondary organic aerosols (SOAs) in the atmosphere. Although significant efforts have been made to characterize the chemical compounds associated with vehicular exhaust, there is still a wealth of compounds that are unable to be detected, posing uncertainties in estimating their contribution to atmospheric reactivity. In this study, by improving laser-induced fluorescence techniques, we achieved the first-ever direct measurement of the total OH reactivity (TOR) from light-duty diesel truck (LDDT) exhaust with different emission standards. We found that the TOR from the LDDT exhaust was 80-130 times the TOR from the gasoline exhaust measured in Japan. Unexpectedly, we discovered increased TOR emissions along with upgrading emission standards, possibly as a collective result of high combustion temperature in the engine and the oxidation catalysts in the exhaust after-treatment that favor production of highly oxidized organics in the stricter emission standard. Most of these oxidized organics are unable to be speciated by routine measurements, resulting in the missing OH reactivity increasing rapidly from 1.91% for China III to 42.0% for China V LDDT. Upgrading the emission standard failed to reduce the TOR from LDDT exhaust, which may inadvertently promote the contribution of LDDT to the formation of ozone and SOA pollution in China.
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http://dx.doi.org/10.1021/acs.est.2c02944DOI Listing
July 2022

A biphosphine copolymer encapsulated single-site Rh catalyst for heterogeneous regioselective hydroaminomethylation of alkenes.

Chem Commun (Camb) 2022 Jul 19;58(58):8093-8096. Epub 2022 Jul 19.

State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, No. 18, Tianshui Middle Road, Lanzhou, 730000, P. R. China.

A novel porous organic polymer catalyst with encapsulated single-site Rh ([email protected]&-3vPPh) was developed and employed in heterogeneous hydroaminomethylation of alkenes, affording the corresponding amines in good to excellent regioselectivity and catalytic activity by a one-pot method. The combined actions of hierarchical pore confinement and the biphosphine ligand derived from the POP catalyst contributed to the improved regioselectivity.
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http://dx.doi.org/10.1039/d2cc02469aDOI Listing
July 2022

Gas chromatography-mass spectrometry analysis of floral fragrance-related compounds in scented rose (Rosa hybrida) varieties and a subsequent evaluation on the basis of the analytical hierarchy process.

Plant Physiol Biochem 2022 Aug 15;185:368-377. Epub 2022 Jun 15.

College of Landscape Architecture, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address:

Scented rose (Rosa hybrida) varieties are valued as ornamentals, but they also contain volatile organic compounds (VOCs) that produce pleasant aromas. In plants, aromas are produced via metabolism during growth, and each aroma compound has a unique function. In this study, the floral aroma compounds of diverse scented rose varieties were analyzed and classified. The VOCs of different rose varieties were qualitatively and quantitatively analyzed via headspace solid-phase microextraction combined with gas chromatography and mass spectrometry. The test materials were the mature flowers of 55 scented rose varieties that were cultivated under identical conditions. Seventeen important aroma compounds were selected and an analytical hierarchy process (AHP)-based method was developed to identify the most suitable essential oil resources, aromatherapy resources, and healthcare resources. A floral fragrance evaluation model was established for the comprehensive evaluation of the scented rose varieties. The 55 varieties were classified into three grades according to their suitability for each use. 'Soeur Emmanuelle', 'Wollerton Old Hall', 'Accademia', and 'Tianmidemeng' were revealed to be suitable essential oil, aromatherapy, and healthcare resources. On the basis of their aroma compound types, the fifty-five rose varieties were divided into eight groups. The results of this study provide the theoretical basis for the classification of rose flower aromas as well as the rational use of diverse rose varieties to further develop the rose industry.
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http://dx.doi.org/10.1016/j.plaphy.2022.06.007DOI Listing
August 2022

Forsythiaside A improves Influenza A virus infection through TLR7 signaling pathway in the lungs of mice.

BMC Complement Med Ther 2022 Jun 22;22(1):164. Epub 2022 Jun 22.

Department of Microbiology and Immunology, College of Basic Medicine and Public Hygiene, Jinan University, Guangzhou, China.

Background: Influenza A virus infection due to drug resistance and side effects of the conventional antiviral drugs yet remains a serious public health threat for humans and animals. Forsythiaside A is an effective ingredient isolated from the Chinese herbal medicine forsythia. It has various pharmacological effects and has a good therapeutic effect against a variety of infectious diseases. This study aimed to further explore the immunological mechanism of Forsythiaside A in the treatment of influenza virus-infected mice and its effect on the Toll-like receptor 7 (TLR7) signaling pathway in the lungs of these mice.

Methods: C57/BL6J mice and TLR7 mice were infected with the FM1 strains (H1N1 and A/FM/1/4) of the Influenza A virus. Each group of experimental mice were divided into the mock, virus, oseltamivir, and Forsythiaside A groups. Weight change, lung index change, and the mRNA and protein expression levels of key factors in the TLR7 signaling pathway were detected. Flow cytometry was used to detect the changes in the Th1/Th2 and Th17/Treg ratios.

Results: After infection with the Influenza A virus, the weight loss of C57/BL6J mice treated with forsythoside A and oseltamivir decreased, and the pathological tissue sections showed that the inflammatory damage was reduced. The expression levels of the key factors, TLR7, myeloid differentiation factor 88(Myd88), and nuclear factor-kappa B (NF-κB) in the TLR7 signaling pathway were significantly reduced. Flow cytometry showed that Th1/Th2 and Th17/Treg ratios decreased after Forsythiaside A treatment. In the TLR7 mice, there was no significant change after Forsythiaside A treatment in the virus group.

Conclusions: Forsythiaside A affects the TLR7 signaling pathway in mouse lung immune cells and reduces the inflammatory response caused by the Influenza A virus FM1 strain in mouse lungs.
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http://dx.doi.org/10.1186/s12906-022-03644-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214192PMC
June 2022

Biomechanical effects of interbody cage height on adjacent segments in patients with lumbar degeneration: a 3D finite element study.

J Orthop Surg Res 2022 Jun 21;17(1):325. Epub 2022 Jun 21.

Department of Orthopedics, Huashan Hospital, Fudan University, No. 12, Middle Wulumuqi Road, Jing'an District, Shanghai, 200040, China.

Objective: To investigate the biomechanical effects of interbody cage height on adjacent segments in patients with lumbar degeneration undergoing transforaminal lumbar interbody fusion (TLIF) surgery, so as to provide references for selection of interbody cage.

Methods: The finite element model of normal lower lumbar spine (L3-S1) was built and validated, then constructed three different degenerative segments in L3-L4, and the cages with different height (8, 10, 12, 14 mm) were implanted into L4-L5 disc. All the twelve models were loaded with pure moment of 7.5 N m to produce flexion, extension, lateral bending and axial rotation motions on lumbar spine, and the effects of cage height on range of motion (RoM) and intervertebral pressure in lumbar spine were investigated.

Results: The RoM of adjacent segments and the maximum stress of intervertebral discs increased with the increase in cage height, but this trend was not obvious in mild and moderate degeneration groups. After implantation of four different height cages (8, 10, 12, 14 mm), the RoM of L3/L4 segment reached the maximum during extension. The RoM of mild degeneration group was 2.07°, 2.45°, 2.48°, 2.54°, that of moderate degeneration group was 1.79°, 1.97°, 2.05°, 2.05°, and that of severe degeneration group was 1.43°, 1.66°, 1.74°, 1.74°. The stress of L3-L4 intervertebral disc reached the maximum during flexion. The maximum stress of L3-L4 intervertebral disc was 20.16 MPa, 20.28 MPa, 20.31 MPa and 20.33 MPa in the mild group, 20.58 MPa, 20.66 MPa, 20.71 MPa and 20.75 MPa in the moderate group, and 21.27 MPa, 21.40 MPa, 21.50 MPa and 21.60 MPa in the severe group.

Conclusion: For patients with mild-to-moderate lumbar degenerative disease who need to undergo TLIF surgery, it is recommended that the height of fusion cage should not exceed the original intervertebral space height by 2 mm, while for patients with severe degeneration, a fusion cage close to the original intervertebral height should be selected as far as possible, and the intervertebral space should not be overstretched.
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http://dx.doi.org/10.1186/s13018-022-03220-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210615PMC
June 2022

Breastfeeding has no protective effects on the development of coronary artery lesions in Kawasaki disease: a retrospective cohort study.

BMC Pediatr 2022 06 20;22(1):353. Epub 2022 Jun 20.

Department of Cardiology, Children's Hospital of Soochow University, No. 92, Zhongnan Street, Suzhou, China.

Background: Kawasaki disease (KD) is a self-limiting vasculitis with an unknown etiology. It has been reported that breastfeeding has a potential protective effect on KD development. However, whether breastfeeding has an effect on the development of coronary artery lesions (CALs) remains unclear.

Methods: We retrospectively reviewed the medical records of patients with the main diagnosis of KD hospitalized in our hospital from May 2017 to November 2018. Standardized telephone interviews were carried out to obtain feeding practices before KD was onset.

Results: Two hundred and ninety-three (51.6%) were exclusively breastfed, 223 (39.3%) were partially breastfed and 52 (9.2%) were formula fed. There were no significant differences in the characteristics regarding age, gender, incomplete KD, intravenous immunoglobulin (IVIG) resistance, and the laboratory variables among the three groups. With formula feeding as a reference, patients exclusively breastfed and partially breastfed seemed to have a higher incidence of CALs, even after adjusting confounders, but were not statistically significant. After grouping patients who were older than six months into formula feeding, partial breastfeeding for < 2 months, partial breastfeeding for ≥ 2 and < 4 months, partial breastfeeding for ≥ 4 and < 6 months and exclusively breastfeeding based on the length of breastfeeding, the results remained the same (P > 0.05).

Conclusions: Breastfeeding has no protective effect on the development of CALs in KD.
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http://dx.doi.org/10.1186/s12887-022-03422-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208131PMC
June 2022

The regulatory effect of pulmonary lymphatic drainage on silicosis fibrosis.

Ecotoxicol Environ Saf 2022 Aug 15;241:113758. Epub 2022 Jun 15.

School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063210, China. Electronic address:

Silicosis is a fibrotic disease caused by long-term inhalation of SiO particles that currently has no effective treatment. Earlier studies have suggested that pulmonary lymphatic vessels play a key role in the transport of silica but have not address the long-term effects of altered pulmonary lymphatic drainage on silicosis. Here, we investigated the impact of impaired pulmonary lymphatic drainage on silicosis. In the past, lymphatic drainage disorders were established mainly through the use of VEGF inhibitors. For the first time, we established a model of pulmonary lymphatic drainage disorder by ligating the thoracic duct in rats. Impaired pulmonary lymphatic drainage was found to aggravate inflammation and oxidative damage in silicosis rats and accelerate silicosis progression. Next, we investigated the effect of pulmonary lymphatic drainage on silicosis. We have demonstrated the effect of sodium tanshinone IIA sulfonate(STS) on lymphangiogenesis, which revealed that STS promotes lymphangiogenesis and can delay inflammation, oxidative damage, and fibrosis progression in silicosis rats by promoting the pulmonary lymphatic drainage response, and this effect is mediated by the VEGFR-3/PI3K/AKT signaling pathway. These findings suggest that pulmonary lymphogenesis plays an important role in silicosis pathogenesis, and targeted intervention in pulmonary lymphangiogenesis may be a potential strategy for treating of silicosis in the future.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113758DOI Listing
August 2022

Characteristics, formation, and sources of PM in 2020 in Suzhou, Yangtze River Delta, China.

Environ Res 2022 09 30;212(Pt D):113545. Epub 2022 May 30.

Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing, 210044, PR China. Electronic address:

Here we present seasonal chemical characteristics, formations, sources of PM in the year 2020 in Suzhou, Yangtze River Delta, China. Expectedly, organic matter (OM) found to be the most dominant component of PM, with a year-average value of 10.3 ± 5.5 μg m, followed by NO (6.7 ± 6.5 μg m), SO (3.3 ± 2.5 μg m), NH (3.2 ± 2.8 μg m), EC (1.1 ± 1.3 μg m), Cl (0.57 ± 0.56 μg m), Ca (0.55 ± 0.91 μg m), K (0.2 ± 1.0 μg m), Na (0.18 ± 0.45 μg m), and Mg (0.09 ± 0.15 μg m). Seasonal variations of PM showed the highest average value in spring, followed by winter, fall, and summer. Meanwhile, the formation mechanisms of the major PM species (NO, SO, and OM) varied in seasons. Interestingly, NO may have the highest conversion rate to NO in spring, which might be linked with the nighttime chemistry due to the high relative humidity. Moreover, OM in summer was mainly produced by the daytime oxidation of volatile organic compounds, while local primary organic aerosols might play a significant role in other seasons. Source apportionment showed that the more-aged PM contributed significantly to the PM mass (42%), followed by the dust-related PM (38%) and the less-aged PM (21%). Potential contribution source function (PSCF) results indicated that aged PM were less affected by transportation than dust-related PM.
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http://dx.doi.org/10.1016/j.envres.2022.113545DOI Listing
September 2022

Sources and processes of organic aerosol in non-refractory PM and PM during foggy and haze episodes in an urban environment of the Yangtze River Delta, China.

Environ Res 2022 09 28;212(Pt D):113557. Epub 2022 May 28.

Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing, China; State Environmental Protection Key Laboratory of Formation and Prevention of Urban Air Pollution Complex, Shanghai Academy of Environment Sciences, Shanghai, China. Electronic address:

Organic aerosol (OA) generally accounts for a large fraction of fine particulate matter (PM) in the urban atmosphere. Despite significant advances in the understanding their emission sources, transformation processes and optical properties in the submicron aerosol fraction (PM), larger size fractions - e.g., PM - still deserve complementary investigations. In this study, we conducted a comprehensive analysis on sources, formation process and optical properties of OA in PM and PM under haze and foggy environments in the Yangtze River Delta (eastern China), using two aerosol chemical speciation monitors, as well as a photoacoustic extinctiometer at 870 nm. Positive matrix factorization analysis - using multilinear engine (ME2) algorithm - was conducted on PM and PM organic mass spectra. Four OA factors were identified, including three primary OA (POA) factors, i.e., hydrocarbon-like OA (HOA), cooking OA (COA), and biomass burning OA (BBOA), and a secondary OA (SOA) factor, i.e., oxidized oxygenated OA (OOA). An enhanced PM COA concentration was clearly observed during cooking peak hours, suggesting important contribution of fresh cooking emissions on large-sized particles (i.e., PM). The oxidation state and concentration of PM HOA were higher than that in PM, suggesting that large-sized HOA particles might be linked to oxidized POA. High contribution (44%) of large-sized OOA to non-refractory PM mass was observed during haze episodes. During foggy episodes, PM and PM OOA concentrations increased as a positive relationship over time, along with an exponential increase in the PM-OOA to PM-OOA ratio. Meanwhile, OOA loadings increased with the aerosol liquid water content (ALWC) during foggy episodes. Random forest cross-validation analysis also supported the important influence of ALWC on OOA variations, supporting substantial impact of aqueous process on SOA formation during haze and/or foggy episodes. Obtained results also indicated high OOA contributions (21%-36%) and low POA contributions (6%-14%) to the PM scattering coefficient during haze and foggy episodes, respectively. Finally, we could illustrate that atmospheric vertical diffusion and horizontal transport have important but different effects on the concentrations of different primary and secondary OA factors in different particle size fractions.
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http://dx.doi.org/10.1016/j.envres.2022.113557DOI Listing
September 2022

Robust network topologies for temperature-inducible bioswitches.

J Biol Eng 2022 May 23;16(1):12. Epub 2022 May 23.

The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China.

Background: Thermoinducible bioswitches are unique in that the all-or-none switch response is triggered by temperature, which is a global factor that impacts all biochemical reaction processes. To date, temperature-inducible bioswitches rely exclusively on special thermal sensing biomolecules of DNA, RNA, proteins and lipids whose conformations are critically temperature dependent.

Method: This paper extends the traditional thermal switch by utilizing purposely designed network topologies of biomolecular interactions to achieve the switching function. By assuming the general Arrhenius law for biochemical reactions, we explore the full space of all three-node genetic interaction networks to screen topologies capable of thermal bioswitches. Three target bioswitches, i.e., thermal-inducible Off-On, cold-inducible On-Off, and hybrid Off-On-Off double switches, are considered separately.

Conclusions: We identify the minimal and core network skeletons that are basic and essential for building robust high-performance bioswitches: three Off-On motifs, three On-Off motifs, and an incoherent feedforward motif for an Off-On-Off double switch. Functional topologies are implicitly preferential in choosing parameter values to achieve the target functions. The scenario of the topology-based bioswitch we propose here is an extension of molecule-based bioswitches and would be valuable in aiding the rational design and synthesis of efficient high-performance thermal bioswitches.
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http://dx.doi.org/10.1186/s13036-022-00290-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9128120PMC
May 2022

Field Detection of Highly Oxygenated Organic Molecules in Shanghai by Chemical Ionization-Orbitrap.

Environ Sci Technol 2022 May 20. Epub 2022 May 20.

Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, IRCELYON, 69626 Villeurbanne, France.

Secondary organic aerosol, formed through atmospheric oxidation processes, plays an important role in affecting climate and human health. In this study, we conducted a comprehensive campaign in the megacity of Shanghai during the 2019 International Import Expo (EXPO), with the first deployment of a chemical ionization─Orbitrap mass spectrometer for ambient measurements. With the ultrahigh mass resolving power of the Orbitrap mass analyzer (up to 140,000 Th/Th) and capability in dealing with massive spectral data sets by positive matrix factorization, we were able to identify the major gas-phase oxidation processes leading to the formation of oxygenated organic molecules (OOM) in Shanghai. Nine main factors from three independent sub-range analysis were identified. More than 90% of OOM are of anthropogenic origin and >60% are nitrogen-containing molecules, mainly dominated by the RO + NO and/or NO chemistry. The emission control during the EXPO showed that even though the restriction was effectual in significantly lowering the primary pollutants (20-70% decrease), the secondary oxidation products responded less effectively (14% decrease), or even increased (50 to >200%) due to the enhancement of ozone and the lowered condensation sink, indicating the importance of a stricter multi-pollutant coordinated strategy in primary and secondary pollution mitigation.
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http://dx.doi.org/10.1021/acs.est.1c08346DOI Listing
May 2022

Moderately Reducing Nitrogen Application Ameliorates Salt-Induced Growth and Physiological Damage on Forage Bermudagrass.

Front Plant Sci 2022 29;13:896358. Epub 2022 Apr 29.

Coastal Salinity Tolerant Grass Engineering and Technology Research Center, Ludong University, Yantai, China.

Nitrogen (N) application is one of the most effective methods to alleviate salt-induced damage on plants. Forage bermudagrass has higher utilization potential on saline soil, but whether its N requirement changed under high salt stress has not been studied. Through examining plant growth-related traits, salt-stress-responsive physiological traits, photosynthesis, N metabolism, and forage quality supplied with different N concentrations under high salt stress (200 mM NaCl), we noticed that the optimum N requirement of forage bermudagrass reduced. When supplied with 10 mM N under higher salt stress, plants had a similar biomass, turf color, and chlorophyll content with plants supplied with 15 mM N, accompanied by a lower firing rate and Na content of leaves. The N content, crude protein, crude fat content, the expression of (ammonium transporters), (nitrate reductase), (glutamine synthetase), and (glutamate synthetase), the chlorophyll fluorescence curve, and parameters of leaves (e.g., PI; PI; ABS/RC; TRo/RC; ETo/RC) all peaked under 10 mM N under high salt stress instead of 15 mM N. Through exploring the proper N application under higher salt stress and its alleviation mechanisms, our results indicated that moderate reduction in N application under high salt level had a maximum promotion effect on the salt tolerance of forage bermudagrass without growth or forage quality inhibition. These response mechanisms obtained can provide a useful reference for N application in moderation rather than in excess on forage bermudagrass, especially in higher salinity areas.
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http://dx.doi.org/10.3389/fpls.2022.896358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100817PMC
April 2022

Chromosome-level genome of Pedinomonas minor (Chlorophyta) unveils adaptations to abiotic stress in a rapidly fluctuating environment.

New Phytol 2022 08 8;235(4):1409-1425. Epub 2022 Jun 8.

State Key Laboratory of Agricultural Genomics, BGI-Shenzhen, Shenzhen, 518083, China.

The Pedinophyceae (Viridiplantae) comprise a class of small uniflagellate algae with a pivotal position in the phylogeny of the Chlorophyta as the sister group of the 'core chlorophytes'. We present a chromosome-level genome assembly of the freshwater type species of the class, Pedinomonas minor. We sequenced the genome using Pacbio, Illumina and Hi-C technologies, performed comparative analyses of genome and gene family evolution, and analyzed the transcriptome under various abiotic stresses. Although the genome is relatively small (55 Mb), it shares many traits with core chlorophytes including number of introns and protein-coding genes, messenger RNA (mRNA) lengths, and abundance of transposable elements. Pedinomonas minor is only bounded by the plasma membrane, thriving in temporary habitats that frequently dry out. Gene family innovations and expansions and transcriptomic responses to abiotic stresses have shed light on adaptations of P. minor to its fluctuating environment. Horizontal gene transfers from bacteria and fungi have possibly contributed to the evolution of some of these traits. We identified a putative endogenization site of a nucleocytoplasmic large DNA virus and hypothesized that endogenous viral elements donated foreign genes to the host genome, their spread enhanced by transposable elements, located at gene boundaries in several of the expanded gene families.
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http://dx.doi.org/10.1111/nph.18220DOI Listing
August 2022

Effects of innovation efficiency of high-tech industries on CO2 emissions in China: A spatial Durbin econometric analysis.

PLoS One 2022 10;17(5):e0264017. Epub 2022 May 10.

School of Finance, Harbin University of Commerce, Heilongjiang, PR China.

As a responsible country, China should take on heavy responsibility of energy conservation and emission reduction, and it is an inevitable choice for China to develop high-tech industries and improve the innovation efficiency of high-tech industries in order to alleviate the current environmental pressure. Therefore, this paper takes the panel data of 30 provinces in China from 2005 to 2016 as the research sample, on the basis of using DEA Global-Malmquist index to measure the innovation efficiency of high-tech industries, it constructs three spatial weight matrices, by including spatial geography, spatial economic geography nesting and innovation, and then it uses Spatial Durbin Model to empirically analyze the effect of innovation efficiency of high-tech industry on CO2 emissions in China from spatial perspective. The results indicate: firstly, in China, CO2 emissions gradually increase from 2006 to 2012, however, some provinces have declined after 2012. And CO2 emissions present a descending trend from eastern coastal area to central and western region. Secondly, affected by "warning effect", CO2 emissions show a significant negative spatial spillover effect. Thirdly, the overall level of innovation efficiency of high-tech industries in China is not high, and its impact on CO2 emissions is not a simple linear relationship, but shows an "inverted N-shaped" curvilinear relation, and its decomposition index EC and TC also have similar characteristics. Obviously, the research in this paper provides a necessary theoretical support for China and some emerging developing countries to rational formulating and effective implementing the energy conservation and emission reduction policies.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0264017PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089925PMC
May 2022

Evaluation of Nursing Effect of Pelvic Floor Rehabilitation Training on Pelvic Organ Prolapse in Postpartum Pregnant Women under Ultrasound Imaging with Artificial Intelligence Algorithm.

Comput Math Methods Med 2022 25;2022:1786994. Epub 2022 Apr 25.

Settlement Center, Hunan Maternal and Child Health Hospital, Changsha, 410008 Hunan, China.

This study was aimed at exploring the application value of ultrasound technology and rehabilitation training based on artificial intelligence algorithm in postpartum recovery of pelvic organ prolapse. Sixty patients diagnosed as mild and moderate pelvic organ prolapse by pelvic organ prolapse quantification evaluation were selected as the research objects. The patients were randomly divided into experimental group (30 cases) and control group (30 cases). The patients in the control group were given routine guidance and postpartum health education 42 days after delivery and given no pelvic floor rehabilitation training, waiting for natural recovery. 42 days after delivery, the patients in the experimental group received pelvic floor rehabilitation training based on the patients in the control group. All patients underwent ultrasonography, the convolution neural network (CNN) algorithm was used for image denoising and edge feature extraction, and the performance of the algorithm was evaluated by the Dice coefficient, positive predictive value, sensitivity, and Hausdorff distance. The thickness of levator ani muscle, anterior and posterior diameter of perineal hiatus, pelvic floor muscle strength, and imaging data were compared between the two groups. The results revealed that the thickness of levator ani muscle in the experimental group was significantly greater than that in the control group after one month and three months of treatment (0.633 ± 0.26 cm vs. 0.519 ± 0.234 cm, 0.7 ± 0.214 cm vs. 0.507 ± 0.168 cm, < 0.05). After one month and three months of treatment, the anterior and posterior diameter of perineal fissure in the experimental group was obviously smaller than that in the control group (4.76 ± 0.513 cm vs. 5.002 ± 0.763 cm, 4.735 ± 0.614 cm vs. 4.987 ± 0.581 cm, < 0.05). The pelvic floor muscle strength of the experimental group was remarkably higher than that of the control group after one month and three months of treatment (3.183 ± 1.47 vs. 2.41 ± 1.57, 3.365 ± 1.53 vs. 2.865 ± 1.69, < 0.05). The ultrasonic image was clearer, the focus was more prominent, and the image quality was significantly improved after being processed by artificial intelligence algorithm. The Dice coefficient, positive predictive value, sensitivity, and Hausdorff distance of the proposed algorithm were better than those of the traditional algorithm. Thus, artificial intelligence algorithm had a good effect in ultrasonic image processing. Pelvic floor rehabilitation training had a good effect on postpartum nursing of patients with pelvic organ prolapse.
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http://dx.doi.org/10.1155/2022/1786994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061012PMC
May 2022

Improved hydrogen evolution performance by engineering bimetallic AuPd loaded on amino and nitrogen functionalized mesoporous hollow carbon spheres.

RSC Adv 2022 Apr 19;12(19):11732-11739. Epub 2022 Apr 19.

College of Material Science and Engineering, Key Laboratory of Advanced Structural Materials, Ministry of Education, Changchun University of Technology Changchun 130012 China

A highly efficient heterogeneous catalyst was synthesized by delicate engineering of NH-functionalized and N-doped hollow mesoporous carbon spheres (NH-N-HMCS), which was used for supporting AuPd alloy nanoparticles with ultrafine size and good dispersion (denoted as AuPd/NH-N-HMCS). Without using any additives, the prepared AuPd/NH-N-HMCS catalytic formic acid dehydrogenation possesses superior catalytic activity with an initial turnover frequency value of 7747 mol H per mol catalyst per h at 298 K. The excellent performance of AuPd/NH-N-HMCS derives from the unique hollow mesoporous structure, the small particle sizes and high dispersion of AuPd nanoparticles and the modified Pd electronic structure in the AuPd/NH-N-HMCS composite, as well as the synergistic effect of the modified support.
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http://dx.doi.org/10.1039/d2ra01191cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016496PMC
April 2022

Vitronectin, a Novel Urinary Proteomic Biomarker, Promotes Cell Pyroptosis in Juvenile Systemic Lupus Erythematosus.

Mediators Inflamm 2022 13;2022:8447675. Epub 2022 Apr 13.

The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China.

Objective: Identifying new markers of juvenile systemic lupus erythematosus (JSLE) is critical event to predict patient stratification and prognosis. The aim of the present study is to analyze alteration of urinary protein expression and screen potential valuable biomarkers in juvenile systemic lupus erythematosus (JSLE).

Methods: The urine was collected from the patients with or without JSLE and detected by mass spectrometry to analyze proteomic changes. ELISA was used to verify the Vitronectin (VTN) changes in a new set of patients. The clinical correlation was performed to analyze between VTN and clinical pathological parameters. WB and ELISA were used to analyze VTN-mediated cell pyroptosis.

Results: Herein, we have identified a group of 105 differentially expressed proteins with ≥1.3-fold upregulation or ≤0.77-fold downregulation in JSLE patients. These proteins were involved in several important biological processes, including acute phase inflammatory responses, complement activation, hemostasis, and immune system regulation through Gene Ontology and functional enrichment analysis. Interestingly, urinary ephrin type-A receptor 4 (EPHA4) and VTN were significantly reduced in both inactive and active JSLE patients, and VTN treatment in THP-1 derived macrophages led to a significant increased cell pyroptosis by activation of Nod-like receptor family protein 3 (NLRP3) inflammasomes, resulting in caspase-1 activation, cleaved gasdermin D (GSDMD), and IL-18 secretion. Most importantly, the urinary VTN was also linearly correlated with clinical characteristics of JSLE, implying that VTN could be a specific diagnostic biomarker to distinguish inactive and active JSLE.

Conclusion: This study provided a novel role of VTN in pyroptosis in JSLE through the urinary proteomic profile for JSLE, which could be a nonintrusive monitoring strategy in clinical diagnosis.
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http://dx.doi.org/10.1155/2022/8447675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020974PMC
April 2022

The Cycas genome and the early evolution of seed plants.

Nat Plants 2022 04 18;8(4):389-401. Epub 2022 Apr 18.

Sichuan Cycas panzhihuaensis National Nature Reserve, Panzhihua, China.

Cycads represent one of the most ancient lineages of living seed plants. Identifying genomic features uniquely shared by cycads and other extant seed plants, but not non-seed-producing plants, may shed light on the origin of key innovations, as well as the early diversification of seed plants. Here, we report the 10.5-Gb reference genome of Cycas panzhihuaensis, complemented by the transcriptomes of 339 cycad species. Nuclear and plastid phylogenomic analyses strongly suggest that cycads and Ginkgo form a clade sister to all other living gymnosperms, in contrast to mitochondrial data, which place cycads alone in this position. We found evidence for an ancient whole-genome duplication in the common ancestor of extant gymnosperms. The Cycas genome contains four homologues of the fitD gene family that were likely acquired via horizontal gene transfer from fungi, and these genes confer herbivore resistance in cycads. The male-specific region of the Y chromosome of C. panzhihuaensis contains a MADS-box transcription factor expressed exclusively in male cones that is similar to a system reported in Ginkgo, suggesting that a sex determination mechanism controlled by MADS-box genes may have originated in the common ancestor of cycads and Ginkgo. The C. panzhihuaensis genome provides an important new resource of broad utility for biologists.
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http://dx.doi.org/10.1038/s41477-022-01129-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023351PMC
April 2022

Association Between Roussouly Classification and Characteristics of Lumbar Degeneration.

World Neurosurg 2022 Jul 12;163:e565-e572. Epub 2022 Apr 12.

Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai, China. Electronic address:

Objective: Different sagittal morphologies are associated with various lumbar degenerative diseases. In this paper, we aim to investigate the association between Roussouly classification and the characteristics of lumbar degeneration.

Methods: A total of 273 patients with lumbar degeneration diseases were included in this cross-sectional study. The patients were divided into 4 groups according to the Roussouly classification. The degeneration of intervertebral disc (IVD) and facet joint was evaluated by Pfirrmann and Pathria grading scales, respectively. Our goal was to elucidate the degenerative characteristics of diverse lumbar sagittal morphologies by comparing the degeneration degree of IVDs and facet joints between 4 groups.

Results: The differences in sacral slope and lumbar lordosis were significant between the 4 groups (P < 0.05). Type 2 lumbar spine had the highest proportion of high-grade disc degeneration compared with other subtypes (P < 0.05), types 1 and 2 had a higher proportion of high-grade degeneration than types 3 and 4 (P < 0.05). However, type 4 lumbar spine had the highest proportion of high-grade facet joint degeneration compared with other subtypes (P < 0.05), types 3 and 4 had a higher proportion of high-grade degeneration than types 1 and 2 (P < 0.05).

Conclusions: Risks of advanced disc degeneration are higher for patients with lumbar spine morphologies of Roussouly type 1 or type 2, especially for patients with type 2 lumbar spine, whereas high-grade degeneration of facet joint tends to occur in type 3 and type 4 lumbar spine, especially for individuals with type 4 lumbar spine.
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http://dx.doi.org/10.1016/j.wneu.2022.04.032DOI Listing
July 2022

Gas-to-Aerosol Phase Partitioning of Atmospheric Water-Soluble Organic Compounds at a Rural Site in China: An Enhancing Effect of NH on SOA Formation.

Environ Sci Technol 2022 04 17;56(7):3915-3924. Epub 2022 Mar 17.

Key Lab of Geographic Information Science of the Ministry of Education, School of Geographic Sciences, East China Normal University, Shanghai 200062, China.

Partitioning gaseous water-soluble organic compounds (WSOC) to the aerosol phase is a major formation pathway of atmospheric secondary organic aerosols (SOA). However, the fundamental mechanism of the WSOC-partitioning process remains elusive. By simultaneous measurements of both gas-phase WSOC (WSOCg) and aerosol-phase WSOC (WSOCp) and formic and acetic acids at a rural site in the Yangtze River Delta (YRD) region of China during winter 2019, we showed that WSOCg during the campaign dominantly partitioned to the organic phase in the dry period (relative humidity (RH) < 80%) but to aerosol liquid water (ALW) in the humid period (RH > 80%), suggesting two distinct SOA formation processes in the region. In the dry period, temperature was the driving factor for the uptake of WSOCg. In contrast, in the humid period, the factors controlling WSOCg absorption were ALW content and pH, both of which were significantly elevated by NH through the formation of NHNO and neutralization with organic acids. Additionally, we found that the relative abundances of WSOCp and NHNO showed a strong linear correlation throughout China with a spatial distribution consistent with that of NH, further indicating a key role of NH in WSOCp formation at a national scale. Since WSOCp constitutes the major part of SOA, such a promoting effect of NH on SOA production by elevating ALW formation and WSOCg partitioning suggests that emission control of NH is necessary for mitigating haze pollution, especially SOA, in China.
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http://dx.doi.org/10.1021/acs.est.1c06855DOI Listing
April 2022
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