Publications by authors named "Honglei Wang"

195 Publications

Coronavirus transmissible gastroenteritis virus antagonizes the antiviral effect of the microRNA miR-27b via the IRE1 pathway.

Sci China Life Sci 2021 Nov 19. Epub 2021 Nov 19.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.

Although the functional parameters of microRNAs (miRNAs) have been explored to some extent, the roles of these molecules in coronavirus infection and the regulatory mechanism of miRNAs in virus infection are still unclear. Transmissible gastroenteritis virus (TGEV) is an enteropathgenic coronavirus and causes high morbidity and mortality in suckling piglets. Here, we demonstrated that microRNA-27b-3p (miR-27b-3p) suppressed TGEV replication by directly targeting porcine suppressor of cytokine signaling 6 (SOCS6), while TGEV infection downregulated miR-27b-3p expression in swine testicular (ST) cells and in piglets. Mechanistically, the decrease of miR-27b-3p expression during TGEV infection was mediated by the activated inositol-requiring enzyme 1 (IRE1) pathway of the endoplasmic reticulum (ER) stress. Further studies showed that when ER stress was induced by TGEV, IRE1 acted as an RNase activated by autophosphorylation and unconventionally spliced mRNA encoding a potent transcription factor, X-box-binding protein 1 (Xbp1s). Xbp1s inhibited the transcription of miR-27 and ultimately reduced the production of miR-27b-3p. Therefore, our findings indicate that TGEV inhibits the expression of an anti-coronavirus microRNA through the IRE1 pathway and suggest a novel way in which coronavirus regulates the host cell response to infection.
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http://dx.doi.org/10.1007/s11427-021-1967-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617553PMC
November 2021

Characteristics of air quality in different climatic zones of China during the COVID-19 lockdown.

Atmos Pollut Res 2021 Dec 27;12(12):101247. Epub 2021 Oct 27.

Key Laboratory for Aerosol-Cloud-Precipitation of China Meteorological Administration, Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Nanjing University of Information Science &Technology, Nanjing, 210044, China.

The diverse climate types and the complex anthropogenic source emissions in China lead to the great regional differences of air pollution mechanisms. The COVID-19 lockdown has given us a precious opportunity to understand the effect of weather conditions and anthropogenic sources on the distribution of air pollutants in different climate zones. In this study, to understand the impact of meteorological and socio-economic factors on air pollution during COVID-19 lockdown, we divided 358 Chinese cities into eight climate regions. Temporal, spatial and diurnal variations of six major air pollutants from January 1 to April 18, 2020 were analyzed. The differences in the characteristics of air pollutants in different climate zones were obvious. PM reduced by 59.0%-64.2% in cold regions (North-East China (NEC) and North-Western (NW)), while O surged by 99.0%-99.9% in warm regions (Central South (CS) and Southern Coast (SC)). Diurnal variations of atmospheric pollutants were also more prominent in cold regions. Moreover, PM, PM, CO and SO showed more prominent reductions (20.5%-64.2%) in heating regions (NEC, NW, NCP and MG) than no-heating regions (0.8%-48%). Climate has less influence on NO which dropped by 41.2%-57.1% countrywide during the lockdown. The influences of weather conditions on the atmospheric pollutants in different climate zones were different. The wind speed was not the primary reason for the differences in air pollutants in different climate zones. Temperature, precipitation, and air pollution emissions led to prominent regional differences in air pollutants throughout the eight climates. The effect of temperature on PM, SO, CO, and NO varied obviously with the latitude, at which condition temperature was negatively correlated to PM, SO, CO, and NO in the north but positively in the south. The temperature was positively correlated to ozone in different climate zones, and the correlation was the highest in NEC and the lowest in SC. The rainfall has a strong removal effect on atmospheric pollutants in the climate regions with more precipitation, but it increases the pollutant concentrations in the climate regions with less precipitation. In regions with more emission sources, air pollutants experienced more significant variations and returned to pre-lockdown levels earlier.
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http://dx.doi.org/10.1016/j.apr.2021.101247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548732PMC
December 2021

Digital-intellectual design of microporous organic polymers.

Phys Chem Chem Phys 2021 Oct 20;23(40):22835-22853. Epub 2021 Oct 20.

Institute of Molecular Sciences and Engineering, Institute of Frontier and Interdisciplinary Science, Shandong University, Qingdao 266237, China.

Microporous organic polymers (MOPs) are a new class of microporous materials. Due to their high porosity, large pore volume, and large surface area, MOPs exhibit excellent performance in gas adsorption and storage, membrane separation, ion capture, heterogeneous catalysis, light energy conversion and storage, capacitance, and other fields. However, selecting high-performance materials for specific applications from thousands of candidate MOPs is a key problem. Traditional design strategies for new materials with targeted properties, including trial-and-error and relying on the experiences of domain experts, are time- and cost-consuming. With the rapid development of computation technology and theoretical chemistry, the discovery of new materials is no longer a purely experimental subject. Breaking away from the traditional trial-and-error strategy for materials discovery, materials design is emerging and gaining increasing attention. In addition, the ability to collect "big data" has greatly improved and has further stimulated the development of new methods for materials design and discovery. In this perspective, we examine how data-driven techniques combine artificial intelligence (AI) and human expertise, playing a significant role in the design of MOPs. Such analytics can significantly reduce time-to-insight and accelerate the cost-effective materials discovery, which is the goal for designing future MOPs.
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http://dx.doi.org/10.1039/d1cp03456aDOI Listing
October 2021

Reaction-Induced Phase Separation and Morphology Evolution of Benzoxazine/Epoxy/Imidazole Ternary Blends.

Polymers (Basel) 2021 Aug 31;13(17). Epub 2021 Aug 31.

Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Guangdong Engineering Technology Research Center for High-Performance Organic and Polymer Photoelectric Functional Films, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.

Introducing multiphase structures into benzoxazine (BOZ)/epoxy resins (ER) blends via reaction-induced phase separation has proved to be promising strategy for improving their toughness. However, due to the limited contrast between two phases, little information is known about the phase morphological evolutions, a fundamental but vital issue to rational design and preparation of blends with different phase morphologies in a controllable manner. Here we addressed this problem by amplifying the difference of polymerization activity (PA) between BOZ and ER by synthesizing a low reactive phenol-3,3-diethyl-4,4'-diaminodiphenyl methane based benzoxazine (MOEA-BOZ) monomer. Results indicated that the PA of ER was higher than that of BOZ. The use of less reactive MOEA-BOZs significantly enlarged their PA difference with ER, and thus increased the extent of phase separation and improved the phase contrast. Phase morphologies varied with the content of ER. As for the phase morphological evolution, a rapid phase separation could occur in the initial homogeneous blends with the polymerization of ER, and the phase morphology gradually evolved with the increase in ER conversion until the ER was used up. The polymerization of ER is not only the driving-force for the phase separation, but also the main factor influencing the phase morphologies.
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http://dx.doi.org/10.3390/polym13172945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433963PMC
August 2021

Tungsten Oxide/Reduced Graphene Oxide Aerogel with Low-Content Platinum as High-Performance Electrocatalyst for Hydrogen Evolution Reaction.

Small 2021 Sep 30;17(37):e2102159. Epub 2021 Jul 30.

The Key Laboratory of Low-Carbon Chemistry & Energy Conservation of Guangdong Province, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, State Key Laboratory of Optoelectronic Materials and Technologies, School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou, 510006, P. R. China.

Designing cost-effective, highly active, and durable platinum (Pt)-based electrocatalysts is a crucial endeavor in electrochemical hydrogen evolution reaction (HER). Herein, the low-content Pt (0.8 wt%)/tungsten oxide/reduced graphene oxide aerogel (LPWGA) electrocatalyst with excellent HER activity and durability is developed by employing a tungsten oxide/reduced graphene oxide aerogel (WGA) obtained from a facile solvothermal process as a support, followed by electrochemical deposition of Pt nanoparticles. The WGA support with abundant oxygen vacancies and hierarchical pores plays the roles of anchoring the Pt nanoparticles, supplying continuous mass transport and electron transfer channels, and modulating the surface electronic state of Pt, which endow the LPWGA with both high HER activity and durability. Even under a low loading of 0.81 μg cm , the LPWGA exhibits a high HER activity with an overpotential of 42 mV at 10 mA cm , an excellent stability under 10000-cycle cyclic voltammetry and 40 h chronopotentiometry at 10 mA cm , a low Tafel slope (30 mV dec ), and a high turnover frequency of 29.05 s at η = 50 mV, which is much superior to the commercial Pt/C and the low-content Pt/reduced graphene oxide aerogel. This work provides a new strategy to design high-performance Pt-based electrocatalysts with greatly reduced use of Pt.
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http://dx.doi.org/10.1002/smll.202102159DOI Listing
September 2021

Facile Preparation of MoS Nanocomposites for Efficient Potassium-Ion Batteries by Grinding-Promoted Intercalation Exfoliation.

Small 2021 Aug 16;17(34):e2102263. Epub 2021 Jul 16.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications (NUPT), Nanjing, 210023, China.

Efficient exfoliations of bulk molybdenum disulfide (MoS ) into few-layered nanosheets in pure phase are highly attractive because of the promising applications of the resulted 2D materials in diversified optoelectronic devices. Here, a new exfoliation method is presented to prepare semiconductive 2D hexagonal phase (2H phase) MoS -cellulose nanocrystal (CNC) nanocomposites using grinding-promoted intercalation exfoliation (GPIE). This method with facile grinding of the bulk MoS and CNC powder followed by conventional liquid-phase exfoliation in water can not only efficiently exfoliate 2H-MoS nanosheets, but also produce the 2H-MoS /CNC 2D nanocomposites simultaneously. Interestingly, the intercalated CNC sandwiched in MoS nanosheets increases the interlayer spacing of 2H-MoS , providing perfect conditions to accommodate the large-sized ions. Therefore, these nanocomposites are good anode materials of potassium-ion batteries (KIBs), showing a high reversible capacity of 203 mAh g at 200 mA g after 300 cycles, a good reversible capacity of 114 mAh g at 500 mA g , and a low decay of 0.02% per cycle over 1500 cycles. With these impressive KIB performances, this efficient GPIE method will open up a new avenue to prepare pure-phase MoS and promising 2D nanocomposites for high-performance device applications.
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http://dx.doi.org/10.1002/smll.202102263DOI Listing
August 2021

Directed evolution of alditol oxidase for the production of optically pure D-glycerate from glycerol in the engineered Escherichia coli.

J Ind Microbiol Biotechnol 2021 Aug;48(7-8)

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 Xiqidao, Tianjin Airport Economic Park, Tianjin 300308, China.

D-glycerate is an attractive chemical for a wide variety of pharmaceutical, cosmetic, biodegradable polymers, and other applications. Now several studies have been reported about the synthesis of glycerate by different biotechnological and chemical routes from glycerol or other feedstock. Here, we present the construction of an Escherichia coli engineered strain to produce optically pure D-glycerate by oxidizing glycerol with an evolved variant of alditol oxidase (AldO) from Streptomyces coelicolor. This is achieved by starting from a previously reported variant mAldO and employing three rounds of directed evolution, as well as the combination of growth-coupled high throughput selection with colorimetric screening. The variant eAldO3-24 displays a higher substrate affinity toward glycerol with 5.23-fold than the wild-type AldO, and a 1.85-fold increase of catalytic efficiency (kcat/KM). Then we introduced an isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible T7 expression system in E. coli to overexpress the variant eAldO3-24, and deleted glucosylglycerate phosphorylase encoding gene ycjM to block the consumption of D-glycerate. Finally, the resulting strain TZ-170 produced 30.1 g/l D-glycerate at 70 h with a yield of 0.376 mol/mol in 5-l fed-batch fermentation.
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http://dx.doi.org/10.1093/jimb/kuab041DOI Listing
August 2021

PK/PD Modeling to Assess Rifaximin Clinical Dosage in a Mouse Model of Induced Mastitis.

Front Vet Sci 2021 14;8:651369. Epub 2021 Jun 14.

National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

() is a common pathogen that causes mastitis, an infection of the milk-secreting tissue of the udder, in dairy cows, and presents a huge economic problem for the dairy industry worldwide. Thus, control and treatment of mastitis in dairy cows is vital in order to reduce the costs associated with the disease. The main purpose of the current work was to examine the current dosage of rifaximin for the treatment mastitis in cows caused by using pharmacokinetic/pharmacodynamic integration in a mouse mastitis model. The mouse mastitis model was established via injection of Newbould 305 (400 CFU/gland) into the mouse mammary gland. A single dose of 50, 100, 200, or 400 μg/gland, administered via intramammary infusion, was used to study the pharmacokinetics of rifaximin. The pharmacokinetic parameters were analyzed by non-compartment and non-linear mixed-effect models using Phoenix software (version 8.1; Pharsight, USA). pharmacodynamics was used to examine 18 therapeutic regimens covering various doses ranging from 25 to 800 μg/gland and three dosing intervals of 8, 12, and 24 h per 24 h experiment cycle. The antibacterial effect of rifaximin was elevated with higher concentrations of rifaximin or shorter intervals of administration. The percentage of time that drug concentrations exceeded the MIC during a dose interval (%T > MIC) was generally 100% for rifaximin and was not better than AUC/MIC in the sigmoid model of inhibitory effect. The optimal antibacterial effect was 2logCFU/gland when the magnitude of AUC/MIC reached 14,281.63 h. A total of 14,281.63 h of AUC/MIC was defined as a target value in the Monte Carlo simulation. The clinically recommended dosage regimen of 100 mg/gland every 8 h in 1 day achieved an 82.97% cure rate for the treatment of bovine mastitis caused by infection.
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http://dx.doi.org/10.3389/fvets.2021.651369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236590PMC
June 2021

Glia-derived temporal signals orchestrate neurogenesis in the mushroom body.

Proc Natl Acad Sci U S A 2021 06;118(23)

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China;

Intrinsic mechanisms such as temporal series of transcription factors orchestrate neurogenesis from a limited number of neural progenitors in the brain. Extrinsic regulations, however, remain largely unexplored. Here we describe a two-step glia-derived signal that regulates neurogenesis in the mushroom body (MB). In a temporal manner, glial-specific ubiquitin ligase dSmurf activates non-cell-autonomous Hedgehog signaling propagation by targeting the receptor Patched to suppress and promote the exit of MB neuroblast (NB) proliferation, thereby specifying the correct α/β cell number without affecting differentiation. Independent of NB proliferation, dSmurf also stabilizes the expression of the cell-adhesion molecule Fasciclin II (FasII) via its WW domains and regulates FasII homophilic interaction between glia and MB axons to refine α/β-lobe integrity. Our findings provide insights into how extrinsic glia-to-neuron communication coordinates with NB proliferation capacity to regulate MB neurogenesis; glial proteostasis is likely a generalized mechanism in orchestrating neurogenesis.
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http://dx.doi.org/10.1073/pnas.2020098118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201942PMC
June 2021

Porcine Reproductive and Respiratory Syndrome Virus: Immune Escape and Application of Reverse Genetics in Attenuated Live Vaccine Development.

Vaccines (Basel) 2021 May 10;9(5). Epub 2021 May 10.

State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China.

Porcine reproductive and respiratory syndrome virus (PRRSV), an RNA virus widely prevalent in pigs, results in significant economic losses worldwide. PRRSV can escape from the host immune response in several processes. Vaccines, including modified live vaccines and inactivated vaccines, are the best available countermeasures against PRRSV infection. However, challenges still exist as the vaccines are not able to induce broad protection. The reason lies in several facts, mainly the variability of PRRSV and the complexity of the interaction between PRRSV and host immune responses, and overcoming these obstacles will require more exploration. Many novel strategies have been proposed to construct more effective vaccines against this evolving and smart virus. In this review, we will describe the mechanisms of how PRRSV induces weak and delayed immune responses, the current vaccines of PRRSV, and the strategies to develop modified live vaccines using reverse genetics systems.
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http://dx.doi.org/10.3390/vaccines9050480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150910PMC
May 2021

EDLmAPred: ensemble deep learning approach for mRNA mA site prediction.

BMC Bioinformatics 2021 May 29;22(1):288. Epub 2021 May 29.

Engineering Research Center of Intelligent Control for Underground Space, Ministry of Education, China University of Mining and Technology, Xuzhou, 221116, China.

Background: As a common and abundant RNA methylation modification, N6-methyladenosine (mA) is widely spread in various species' transcriptomes, and it is closely related to the occurrence and development of various life processes and diseases. Thus, accurate identification of mA methylation sites has become a hot topic. Most biological methods rely on high-throughput sequencing technology, which places great demands on the sequencing library preparation and data analysis. Thus, various machine learning methods have been proposed to extract various types of features based on sequences, then occupied conventional classifiers, such as SVM, RF, etc., for mA methylation site identification. However, the identification performance relies heavily on the extracted features, which still need to be improved.

Results: This paper mainly studies feature extraction and classification of mA methylation sites in a natural language processing way, which manages to organically integrate the feature extraction and classification simultaneously, with consideration of upstream and downstream information of mA sites. One-hot, RNA word embedding, and Word2vec are adopted to depict sites from the perspectives of the base as well as its upstream and downstream sequence. The BiLSTM model, a well-known sequence model, was then constructed to discriminate the sequences with potential mA sites. Since the above-mentioned three feature extraction methods focus on different perspectives of mA sites, an ensemble deep learning predictor (EDLmAPred) was finally constructed for mA site prediction. Experimental results on human and mouse data sets show that EDLmAPred outperforms the other single ones, indicating that base, upstream, and downstream information are all essential for mA site detection. Compared with the existing mA methylation site prediction models without genomic features, EDLmAPred obtains 86.6% of the area under receiver operating curve on the human data sets, indicating the effectiveness of sequential modeling on RNA. To maximize user convenience, a webserver was developed as an implementation of EDLmAPred and made publicly available at www.xjtlu.edu.cn/biologicalsciences/EDLm6APred .

Conclusions: Our proposed EDLmAPred method is a reliable predictor for mA methylation sites.
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http://dx.doi.org/10.1186/s12859-021-04206-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164815PMC
May 2021

NSP2 Is Important for Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus to Trigger High Fever-Related COX-2-PGE2 Pathway in Pigs.

Front Immunol 2021 29;12:657071. Epub 2021 Apr 29.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.

In 2006, atypical porcine reproductive and respiratory syndrome (PRRS) caused by a highly pathogenic PRRSV (HP-PRRSV) strain broke out in China. Atypical PRRS is characterized by extremely high fever and high mortality in pigs of all ages. Prostaglandin E2 (PGE2) derived from arachidonic acid through the activation of the rate-limiting enzyme cyclooxygenase type 1/2 (COX-1/2) plays an important role in fever. Here, we showed that HP-PRRSV infection increased PGE2 production in microglia COX-2 up-regulation depending on the activation of MEK1-ERK1/2-C/EBPβ signaling pathways. Then, we screened HP-PRRSV proteins and demonstrated that HP-PRRSV nonstructural protein 2 (NSP2) activated MEK1-ERK1/2-C/EBPβ signaling pathways by interacting with 14-3-3ζ to promote COX-2 expression, leading to PGE2 production. Furthermore, we identified that the amino acid residues 500-596 and 658-777 in HP-PRRSV NSP2 were essential to up-regulate COX-2 expression and PGE2 production. Finally, we made mutant HP-PRRS viruses with the deletion of residues 500-596 and/or 658-777, and found out that these viruses had impaired ability to up-regulate COX-2 and PGE2 production and . Importantly, pigs infected with the mutant viruses had relieved fever, clinical symptoms, and mortality. These data might help us understand the molecular mechanisms underlying the high fever and provide clues for the development of HP-PRRSV attenuated vaccines.
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http://dx.doi.org/10.3389/fimmu.2021.657071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118602PMC
September 2021

Concentration and atmospheric transport of PM-bound polycyclic aromatic hydrocarbons at Mount Tai, China.

Sci Total Environ 2021 Sep 5;786:147513. Epub 2021 May 5.

Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Key Laboratory for Aerosol-Cloud-Precipitation of China Meteorological Administration, Nanjing University of Information Science & Technology, Nanjing 210044, China.

Atmospheric PM-bound polycyclic aromatic hydrocarbons (PAHs) pose a major threat to human health. At present, studies on PAHs in the atmosphere have mostly focused on their concentration levels and source apportionment, whereas studies on the vertical transport of PAHs in the atmosphere are limited. However, the vertical transport of PAHs is important for their diffusion near the ground and their long-range transport at higher altitude. In this study, PM samples were collected simultaneously at the summit and foot of Mount Tai (MT and MT, respectively) from May to June 2017, and the concentrations of 18 PAHs in the samples were determined. The total concentration of PAHs at MT was 2.406 ng m, which was well below the pollution levels of domestic cities, whereas that at MT was as high as 9.068 ng m, which was within the range of pollution levels in domestic cities. The total carcinogenic risk for both MT and MT was within the potential risk range. Given the source of PAHs and the diurnal variation of the planetary boundary layer, the PAHs showed opposite diurnal trends at MT and MT. Vertical transport was an important source of daytime PAHs at MT, and the vertical transport efficiency of PAHs decreased with an increasing ring number; this may be due to the combined effects of gas-particle partitioning and chemical reactions. Furthermore, PAHs originating in the surrounding high-emission provinces can affect the Mount Tai area via atmospheric trans-regional transport, and the BaP/BeP ratio is a useful indicator of the transport distance of PAHs.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147513DOI Listing
September 2021

Quantitative evaluation of the antibacterial effectiveness and efficiency of chitosan considering the effect of neutralization.

Carbohydr Polym 2021 Aug 5;265:117918. Epub 2021 Mar 5.

College of Marine Life Science, Ocean University of China, Qingdao, 266003, China; Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China. Electronic address:

In the present research, an optimized liquid medium which had no neutralizing effect to chitosan was developed. Moreover, magnesium chloride (MgCl) was identified to be able to absolutely neutralize the antibacterial activity of chitosan and its derivatives. Took the two results together, the minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of chitosan were precisely quantified through a further improved method based on the optimized medium and the relation curve between antibacterial rate and reaction time was obtained with the help of MgCl neutralizer. The MBC and MIC of chitosan were all 30 μg/mL against Staphylococcus aureus and Escherichia coli, and 100 μg/mL of chitosan acetate could reach 100 % of antibacterial rate within 3 min. Furthermore, coordination between magnesium ions and chitosan as well as reduced zeta potential of chitosan caused by coordination were inferred to be the neutralizing mechanism of MgCl neutralizer.
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http://dx.doi.org/10.1016/j.carbpol.2021.117918DOI Listing
August 2021

Puerarin protects cardiomyocytes from ischemia-reperfusion injury by upregulating LncRNA ANRIL and inhibiting autophagy.

Cell Tissue Res 2021 Sep 8;385(3):739-751. Epub 2021 May 8.

Department of Cardiovascular Medicine, The First Affiliated Hospital of Henan Science & Technology University, No. 24, Jinghua Road, Jianxi District, 471003, Luoyang, Henan Province, China.

This study analyzed the roles of puerarin and LncRNA ANRIL in myocardial ischemia-reperfusion injury. Hypoxia/reperfusion (H/R) model was established with H9C2 cells. Effects of puerarin of gradient concentrations on cardiomyocytes at different time points of hypoxia and reoxygenation were detected by quantitative real-time polymerase chain reaction (qRT-PCR), cell counting kit-8 (CCK-8), and microscope observation. Effects of puerarin on cardiomyocyte viability, ANRIL expression, contents of lactate dehydrogenase (LDH) and malondialdehyde (MDA), apoptosis, and expressions of autophagy-related genes after H/R injury were determined by CCK-8, quantitative real-time polymerase chain reaction (qRT-PCR), ELISA, flow cytometry, and Western blot, respectively. After cell transfection, the effects of overexpressed and knockdown of ANRIL on cardiomyocytes and H/R-injured cardiomyocytes were examined by rescue experiments. The ischemia-reperfusion (I/R)-injured rat model was established to examine the protective effect of puerarin in vivo. Prolonged hypoxia downregulated ANRIL expression in cardiomyocytes and reduced cardiomyocyte viability. Prolonged reoxygenation increased apoptosis. Both cardiomyocyte viability and ANRIL expression showed a dose-dependent relationship with puerarin. Puerarin reversed the effects of H/R injury on cardiomyocyte viability, ANRIL expression, contents of LDH and MDA, apoptosis, and expressions of autophagy-related genes. Overexpression and knockdown of ANRIL regulated the functions of cardiomyocytes and the expressions of autophagy-related genes. Puerarin reversed the effects of knockdown of ANRIL on H/R-injured cells. The results of In vivo experiments confirmed that puerarin protected myocardial tissues by up-regulating ANRIL and inhibiting autophagy.
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http://dx.doi.org/10.1007/s00441-021-03463-2DOI Listing
September 2021

Antioxidant Blend of Curcumin and Broccoli Seed Extract Exhibits Protective Effect on Neurodegeneration and Promotes Lifespan.

ASN Neuro 2021 Jan-Dec;13:17590914211015033

School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.

Antioxidants and related compounds are anti-inflammatory and exhibit great potential in promoting human health. They are also often considered to be important elements in the process of neurodegeneration. Here we describe a antioxidant blend of Curcumin and Broccoli Seed Extract (BSE). Flies treated with the blend exhibit extended lifespan. RNA-seq analysis of samples from adult fly brains reveals a wide array of new genes with differential expression upon treatment with the blend. Interestingly, abolishing expression of some of the identified genes in dopaminergic (DA) neurons does not affect DA neuron number. Taken together, our findings reveal an antioxidant blend that promotes fly longevity and exhibits protective effect over neurodegeneration, demonstrating the importance of antioxidants in health and pathology.
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http://dx.doi.org/10.1177/17590914211015033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114746PMC
November 2021

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) induces IL-6 production through TAK-1/JNK/AP-1 and TAK-1/NF-κB signaling pathways.

Vet Microbiol 2021 May 26;256:109061. Epub 2021 Mar 26.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China; Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China; Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address:

Porcine reproductive and respiratory syndrome virus (PRRSV) mainly infects monocyte/macrophage lineage and regulates the production of cytokines to influence host immune responses. Interleukin-6 (IL-6) is originally identified as a B-cell stimulatory factor and has important functions in regulating immune response, hemopoiesis, and inflammation. In this study, we verified that highly pathogenic PRRSV (HP-PRRSV) infection up-regulated IL-6 production in vivo and in vitro. Subsequently, we demonstrated that HP-PRRSV infection activated JNK and NF-κB signaling pathways to enhance IL-6 expression. We further showed that TAK-1 was important in the activation of JNK and NF-κB pathways following HP-PRRSV infection. Moreover, AP-1 and NF-κB binding motifs were found in the cloned porcine IL-6 (pIL-6) promoter, and deletion of these motifs abrogated the activation of pIL-6 promoter by HP-PRRSV, suggesting that IL-6 expression is dependent on AP-1 and NF-κB activation. These findings imply that IL-6 induced by HP-PRRSV infection is dependent on the activation of TAK-1/JNK/AP-1 and TAK-1/NF-κB signaling pathways.
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http://dx.doi.org/10.1016/j.vetmic.2021.109061DOI Listing
May 2021

Long Non-Coding RNA MALAT1 Contributed to the Proliferation of PNH Clone in Paroxysmal Nocturnal Hemoglobinuria Patients

Turk J Haematol 2021 08 17;38(3):236-238. Epub 2021 Mar 17.

Tianjin Medical University General Hospital, Department of Hematology, Tianjin, China

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http://dx.doi.org/10.4274/tjh.galenos.2021.2021.0065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386316PMC
August 2021

Characterization of the aerosol chemical composition during the COVID-19 lockdown period in Suzhou in the Yangtze River Delta, China.

J Environ Sci (China) 2021 Apr 22;102:110-122. Epub 2020 Sep 22.

Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Key Laboratory for Aerosol-Cloud-Precipitation of China Meteorological Administration, Nanjing University of Information Science &Technology, Nanjing 210044, China.

To control the spread of COVID-19, rigorous restrictions have been implemented in China, resulting in a great reduction in pollutant emissions. In this study, we evaluated the air quality in the Yangtze River Delta during the COVID-19 lockdown period using satellite and ground-based data, including particle matter (PM), trace gases, water-soluble ions (WSIs) and black carbon (BC). We found that the impacts of lockdown policy on air quality cannot be accurately assessed using MODIS aerosol optical depth (AOD) data, whereas the tropospheric nitrogen dioxide (NO) vertical column density can well reflect the influences of these restrictions on human activities. Compared to the pre-COVID period, the PM, PM, NO, carbon monoxide (CO), BC and WSIs during the lockdown in Suzhou were observed to decrease by 37.2%, 38.3%, 64.5%, 26.1%, 53.3% and 58.6%, respectively, while the sulfur dioxide (SO) and ozone (O) increased by 1.5% and 104.7%. The WSIs ranked in the order of NO > NH > SO > Cl > Ca > K > Mg > Na during the lockdown period. By comparisons with the ion concentrations during the pre-COVID period, we found that the ions NO, NH, SO, Cl, Ca, K and Na decreased by 66.3%, 48.8%, 52.9%, 56.9%, 57.9% and 76.3%, respectively, during the lockdown, in contrast to Mg, which increased by 30.2%. The lockdown policy was found to have great impacts on the diurnal variations of Cl, SO, Na and Ca.
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http://dx.doi.org/10.1016/j.jes.2020.09.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508540PMC
April 2021

Nanoporous and Highly Thermal Conductive Thin Film of Single-Crystal Covalent Organic Frameworks Ribbons.

J Am Chem Soc 2021 Mar 25;143(10):3927-3933. Epub 2021 Feb 25.

Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, School of Chemistry, and State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University, 510275, Guangzhou, China.

Nanoporous materials are widely explored as efficient adsorbents for the storage of gases and liquids as well as for effective low-dielectric materials in large-scale integrated circuits. These applications require fast heat transfer, while most nanoporous substances are thermal insulators. Here, the oriented growth of micrometer-sized single-crystal covalent organic frameworks (COFs) ribbons with nanoporous structures at an air-water interface is presented. The obtained COFs ribbons are interconnected into a continuous and purely crystalline thin film. Due to the robust connectivity among the COFs ribbons, the entire film can be easily transferred and reliably contacted with target supports. The measured thermal conductivity amounts to ∼5.31 ± 0.37 W m K at 305 K, which is so far the highest value for nanoporous materials. These findings provide a methodology to grow and assemble single-crystal COFs into large area ensembles for the exploration of functional properties and potentially lead to new devices with COFs thin films where both porosity and thermal conductivity are desired.
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http://dx.doi.org/10.1021/jacs.0c13458DOI Listing
March 2021

A Robust PtNi Nanoframe/N-Doped Graphene Aerogel Electrocatalyst with Both High Activity and Stability.

Angew Chem Int Ed Engl 2021 Apr 17;60(17):9590-9597. Epub 2021 Mar 17.

The Key Laboratory of Low-Carbon Chemistry & Energy Conservation of Guangdong Province, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, State Key Laboratory of Optoelectronic Materials and Technologies, School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, P. R. China.

Insufficient catalytic activity and stability and high cost are the barriers for Pt-based electrocatalysts in wide practical applications. Herein, a hierarchically porous PtNi nanoframe/N-doped graphene aerogel (PtNiNF-NGA) electrocatalyst with outstanding performance toward methanol oxidation reaction (MOR) in acid electrolyte has been developed via facile tert-butanol-assisted structure reconfiguration. The ensemble of high-alloying-degree-modulated electronic structure and correspondingly the optimum MOR reaction pathway, the structure superiorities of hierarchical porosity, thin edges, Pt-rich corners, and the anchoring effect of the NGA, endow the PtNiNF-NGA with both prominent electrocatalytic activity and stability. The mass and specific activity (1647 mA mg , 3.8 mA cm ) of the PtNiNF-NGA are 5.8 and 7.8 times higher than those of commercial Pt/C. It exhibits exceptional stability under a 5-hour chronoamperometry test and 2200-cycle cyclic voltammetry scanning.
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http://dx.doi.org/10.1002/anie.202015679DOI Listing
April 2021

Tag-Free Site-Specific BMP-2 Immobilization with Long-Acting Bioactivities via a Simple Sugar-Lectin Interaction.

ACS Biomater Sci Eng 2020 04 10;6(4):2219-2230. Epub 2020 Mar 10.

Department of Chemistry and Chemical Biology and New Jersey Center for Biomaterials, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, United States.

The construction of a biomaterial matrix with biological properties is of great importance to developing functional materials for clinical use. However, the site-specific immobilization of growth factors to endow materials with bioactivities has been a challenge to date. Considering the wide existence of glycosylation in mammalian proteins or recombinant proteins, we establish a bioaffinity-based protein immobilization strategy (bioanchoring method) utilizing the native sugar-lectin interaction between concanavalin A (Con A) and the oligosaccharide chain on glycosylated bone morphogenetic protein-2 (GBMP-2). The interaction realizes the site-specific immobilization of GBMP-2 to a substrate modified with Con A while preserving its bioactivity in a sustained and highly efficient way, as evidenced by its enhanced ability to induce osteodifferentiation compared with that of the soluble GBMP-2. Moreover, the surface with Con A-bioanchored GBMP-2 can be reused to stimulate multiple batches of C2C12 cells to differentiate almost to the same degree. Even after 4 month storage at 4 °C in phosphate-buffered saline (PBS), the Con A-bioanchored GBMP-2 still maintains the bioactivity to stimulate the differentiation of C2C12 cells. Furthermore, the ectopic ossification test proves the in vivo bioactivity of bioanchored GBMP-2. Overall, our results demonstrate that the tag-free and site (i.e., sugar chain)-specific protein immobilization strategy represents a simple and generic alternative, which is promising to apply for other glycoprotein immobilization and application. It should be noted that although the lectin we utilized can only bind to d-mannose/d-glucose, the diversity of the lectin family assures that a specific lectin could be offered for other sugar types, thus expanding the applicable scope further.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01730DOI Listing
April 2020

Photo-Thermoelectric Conversion Using Black Silicon with Enhanced Light Trapping Performance far beyond the Band Edge Absorption.

ACS Appl Mater Interfaces 2021 Jan 3;13(1):1818-1826. Epub 2021 Jan 3.

Chair Materials for Electrical Engineering and Electronics, Institute of Materials Science and Engineering and Institute of Micro and Nanotechnologies MacroNano, TU Ilmenau, Gustav-Kirchhoff-Str. 5, 98693 Ilmenau, Germany.

During the past years, much research work has been focused on efficiently harvesting solar energy with black silicon (b-Si). However, semiconductor Si can only utilize solar energy with wavelength smaller than λ = 1110 nm (bandgap = 1.12 eV) for photovoltaic applications or photoelectrochemical conversions. Light with wavelength beyond the band edge (above λ = 1110 nm) cannot be used. Here, we prepared highly conductive b-Si without an apparent optical bandgap by a reactive ion etching process, which can largely absorb light with a wide range wavelength and even far into the near-infrared region (∼2500 nm). The optimized b-Si with surface texture shows the specular reflection rate lower than 0.1% and the average total reflection (specular reflectance + diffuse reflectance) is about 1.1%. Additionally, we briefly introduce the mechanism and reflection principle of surface nanostructured b-Si. By using b-Si structured material, we successfully convert the solar energy to electric power via photo-thermoelectric conversion, especially solar energy exceeding 1110 nm wavelength can also be efficiently used. The excellent light trapping of sunlight shows great potential for photothermal applications, such as photothermal imaging, seawater desalination, and further applications.
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http://dx.doi.org/10.1021/acsami.0c17279DOI Listing
January 2021

Air pollutant variations in Suzhou during the 2019 novel coronavirus (COVID-19) lockdown of 2020: High time-resolution measurements of aerosol chemical compositions and source apportionment.

Environ Pollut 2021 Feb 21;271:116298. Epub 2020 Dec 21.

Suzhou Environmental Monitoring Center, Suzhou, 215000, China.

To control the spread of the 2019 novel coronavirus (COVID-19), China imposed rigorous restrictions, which resulted in great reductions in pollutant emissions. This study examines the characteristics of air pollutants, including PM (particles with aerodynamic diameters < 2.5 μm), gas pollutants, water-soluble ions (WSIs), black carbon (BC) and elements, as well as the source apportionment of PM in Suzhou before, during and after the Chinese New Year (CNY) holiday of 2020 (when China was under an unprecedented state of lockdown to restrict the COVID-19 outbreak). Compared to those before CNY, PM, BC, SNA (sulfate, nitrate and ammonium), other ions, elements, and NO and CO mass concentrations decreased by 9.9%-64.0% during CNY. The lockdown policy had strong (weak) effects on the diurnal variations in aerosol chemical compositions (gas pollutants). Compared to those before CNY, source concentrations and contributions of vehicle exhaust during CNY decreased by 72.9% and 21.7%, respectively. In contrast, increased contributions from coal combustion and industry were observed during CNY, which were recorded to be 2.9 and 1.7 times higher than those before CNY, respectively. This study highlights that the lockdown policy that was imposed in Suzhou during CNY not only reduced the mass concentrations of air pollutants but also modified their diurnal variations and the source contributions of PM, which revealed the complex responses of PM sources to the rare, low emissions of anthropogenic pollutants that occurred during the COVID-19 lockdown.
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http://dx.doi.org/10.1016/j.envpol.2020.116298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832523PMC
February 2021

Hierarchical CoP Nanostructures on Nickel Foam as Efficient Bifunctional Catalysts for Water Splitting.

ChemSusChem 2021 Feb 7;14(4):1094-1102. Epub 2021 Jan 7.

Catalonia Institute for Energy Research (IREC), 08930, Sant Adrià de Besòs, Barcelona, Spain.

A highly active and cheap catalyst is also key to hydrogen production by water splitting. However, most of the high-efficiency catalysts reported to date only are catalytically active for either the hydrogen evolution reaction (HER) or the oxygen evolution reaction (OER), which makes the development of multifunctional catalysts more meaningful. Here, for the first time, Co(CO ) OH 0.11 H O (CHCH) as precursor with different microstructures on the surface of nickel foam (NF) was obtained using a facile hydrothermal method. The CoP/NF catalyst was obtained after thermal phosphating that retained the microhierarchical structure of the precursor and greatly improved the catalytic performance, with a highly efficiency performance as HER and OER dual-functional catalyst. Density functional theory (DFT) calculations showed that the possible reason for the excellent performance of the CoP/NF layered structure is an increase in the number of of surface defects and an increased active surface area. The results reported in this paper show that CoP/NF, a layered bifunctional electrocatalyst, is a cost-effective and efficient water-splitting electrode. This finding can offer the opportunity for the commercial use of excess electric energy for large-scale water splitting hydrogen production.
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http://dx.doi.org/10.1002/cssc.202002624DOI Listing
February 2021

Coordinated Expression of Astaxanthin Biosynthesis Genes for Improved Astaxanthin Production in .

J Agric Food Chem 2020 Dec 8;68(50):14917-14927. Epub 2020 Dec 8.

Tianjin Institute of Industrial Biotechnology, Chinese of Academy of Sciences, Tianjin 300308, China.

Astaxanthin has great potential commercial value in the feed, cosmetics, and nutraceutical industries due to its strong antioxidant capacity. In this study, the strain CAR026 with completely balanced metabolic flow was selected as the starting strain for the production of astaxanthin. The expression of β-carotene ketolase (CrtW) and β-carotene hydroxylase (CrtZ), which catalyze the conversion of β-carotene to astaxanthin, was coordinated, and a bottleneck was eliminated by increasing the copy number of in CAR026. The resulting strain Ast007 produced 21.36 mg/L and 4.6 mg/g DCW of astaxanthin in shake flasks. In addition, the molecular chaperone genes - were regulated to further improve the astaxanthin yield. The best strain Gro-46 produced 26 mg/L astaxanthin with a yield of 6.17 mg/g DCW in shake flasks and 1.18 g/L astaxanthin after 60 h of fermentation under fed-batch conditions. To the best of our knowledge, this is the highest astaxanthin obtained using engineered to date.
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http://dx.doi.org/10.1021/acs.jafc.0c05379DOI Listing
December 2020

Relationship between immune status after ATG treatment and PNH clone evolution in patients with severe aplastic anemia.

J Clin Lab Anal 2021 Mar 28;35(3):e23667. Epub 2020 Nov 28.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China.

Objectives: To investigate the relationship between immune status and paroxysmal nocturnal hemoglobinuria (PNH) clonal evolution of severe aplastic anemia (SAA) patients who received anti-human thymocyte globulin (ATG) treatment.

Methods: The clinical data of 102 SAA patients who received ATG were collected and retrospectively analyzed. The remission rate, remission time, response rate, hematopoietic, and immune status were compared. Malignant clones were also observed.

Results: The remission rate of the group with PNH clones appeared after treatment was significantly higher than the group without PNH clones. The response rate at 12 months of the groups with PNH clones was significantly higher than the group without PNH clones. The recovery of Hb and Ret % of patients with PNH clones was earlier than the patients without PNH clones. The reduction of percentage of CD8 HLA-DR /CD8 and Th1/Th2 ratio of patients with PNH clones was both earlier than the patients without PNH clones. Six patients developed myelodysplastic syndromes (MDS).

Conclusion: In SAA patients with PNH clones, the cytotoxic T-cell function and Th1 cell number recovered more quickly and had better response to IST. A small number of SAA patients with or without PNH clones developed MDS malignant clones.
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http://dx.doi.org/10.1002/jcla.23667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958003PMC
March 2021

Fluorescent Magnetic Iron Oxide NanoparticleEncapsulated Protein Hydrogel Against Doxorubicin-Associated Cardiotoxicity and for Enhanced Cardiomyocyte Survival.

J Biomed Nanotechnol 2020 Jun;16(6):922-930

Doxorubicin (DOX) is a widely used and effective anticancer drug. However, it shows high cardiotoxicity in several patients. The exact biological mechanisms of DOX-induced cardiotoxicity remain unclear. In the present study, we developed and assessed novel injectable hydrogel matrices combined with nanoparticles and secretome biomolecules to reduce DOXinduced cytotoxicity in human stem cell-derived cardiomyocytes. A Fe₂O₃ nanoparticle-loaded biocompatible silk sericin nanocomposite form was fabricated and used as an injectable carrier for secretome for cardiomyocyte metabolism. The formulated hydrogels carrying secretome were analyzed for proliferation, migration, and tube formation of human stem cell-derived cardiomyocytes. Biological analyses revealed that the secretome-encapsulated florescent Fe₃O₂ Silk sericin ([email protected]) hydrogel markedly reduced calcein-PI dual staining in cardiomyocytes, revealing significantly induced apoptosis. Furthermore, we evaluated the mitochondrial membrane potential for DOX and [email protected] hydrogel, and demonstrated apoptosis of the cardiomyocytes in the DOX-alone and [email protected] groups. However, the cardiotoxicity of [email protected] sericin was much lower than that in the DOX group, and was further evaluated via VEGFR and TUNEL analyses. The results indicate that [email protected] hydrogel might serve as an effective treatment agent in cardiac diseases in the future.
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http://dx.doi.org/10.1166/jbn.2020.2945DOI Listing
June 2020

Twenty-two novel mutations in a Chinese cohort of 137 patients with porokeratosis were identified using microfluidics (Fluidigm).

J Dermatol Sci 2021 Jan 24;101(1):75-77. Epub 2020 Oct 24.

School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, China; Shandong Provincial Hospital for Skin Diseases & Shandong Provincial Institute of Dermatology and Venereology, Jinan, Shandong, China; Shandong First Medical University & Shandong Academy of Medical Sciences Jinan, Shandong, China; Binzhou Medical University, Binzhou, Shandong, China.

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http://dx.doi.org/10.1016/j.jdermsci.2020.10.013DOI Listing
January 2021

Clinical characteristics associated with sentinel headache in patients with unruptured intracranial aneurysms.

Interv Neuroradiol 2021 Aug 4;27(4):497-502. Epub 2020 Nov 4.

Department of Neurosurgery, The First Hospital of Jilin University, Changchun, China.

Objective: Sentinel headache (SH) is considered as a signal of the impending rupture of an aneurysm. However, it is difficult to diagnose whether the headaches of patients are associated with unstable aneurysms. Therefore, there is some doubt about the importance of headaches in patients with unruptured intracranial aneurysms (UIAs). This study was performed to explore the existence and clinical characteristics of SH associated with aneurysms.

Methods: Thirty-six patients with a single UIA were collected in this study. Patients were symptomatically categorized into two groups: SH and non-SH. The PHASES scores and patient and aneurysm characteristics were analyzed. Two independent MRI experts who were blinded to the patients' clinical history conducted the analysis of the SWI results.

Results: There were 15 patients with sentinel headache. No significant difference was found in patient's basic information and history. The SH group had a higher PHASES score than the non-SH group (P < 0.05). In univariable analysis, abnormal SWI signals were significantly more frequent in the SH group (P < 0.01) and the inflow angle was significantly lower in the non-SH group (P < 0.05). In multivariable analysis, abnormal signals in SWI were an independent factor associated with SH (P < 0.01).

Conclusions: SH exists in patients with UIAs and may indicate a high risk of aneurysm rupture. Abnormal signals on SWI may serve as a clinical feature to identify aneurysm-related SH and be helpful for the formulation of therapeutic strategy. Aneurysm geometry may also be related to SH but need further studies in the future.
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http://dx.doi.org/10.1177/1591019920971977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580530PMC
August 2021
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