Publications by authors named "Hongjun Liu"

293 Publications

Bio-organic soil amendment promotes the suppression of Ralstonia solanacearum by inducing changes in the functionality and composition of rhizosphere bacterial communities.

New Phytol 2022 May 15. Epub 2022 May 15.

Microbial Ecology Cluster, Genomics Research in Ecology and Evolution in Nature, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, 9747AG, the Netherlands.

Stimulating the development of soil suppressiveness against certain pathogens represents a sustainable solution toward reducing pesticide use in agriculture. However, understanding the dynamics of suppressiveness and the mechanisms leading to pathogen control remain largely elusive. Here, we investigated the mechanisms used by the rhizosphere microbiome induces bacterial wilt disease suppression in a long-term field experiment where continuous application of bio-organic (BF) fertilizers triggered disease suppressiveness when compared to chemical fertilizer (CF) application. We further demonstrated in a greenhouse experiment that the suppressiveness of the rhizosphere bacterial communities was triggered mainly by changes in community composition rather than only by the abundance of the introduced biocontrol strain. Metagenomics approaches revealed that members of the families Sphingomonadaceae and Xanthomonadaceae with the ability to produce secondary metabolites were enriched in the BF plant rhizosphere but only upon pathogen invasion. We experimentally validated this observation by inoculating bacterial isolates belonging to the families Sphingomonadaceae and Xanthomonadaceae into conducive soil, which led to a significant reduction in pathogen abundance and increase in non-ribosomal peptide synthetase (NRPS) gene abundance. We conclude that priming of the soil microbiome with bio-organic fertilizer amendment fostered reactive bacterial communities in the rhizosphere of tomato plants in response to biotic disturbance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.18221DOI Listing
May 2022

Effects of Cartilage Progenitor Cells, Bone Marrow Mesenchymal Stem Cells and Chondrocytes on Cartilage Repair as Seed Cells: An in vitro Study.

Drug Des Devel Ther 2022 28;16:1217-1230. Epub 2022 Apr 28.

Clinical Medical College, Yangzhou University, Yangzhou, People's Republic of China.

Purpose: To determine the effects of cartilage progenitor cells, bone marrow mesenchymal stem cells and chondrocytes on cartilage repair as seed cells.

Methods: Porcine cartilage progenitor cells (CPCs), bone marrow mesenchymal stem cells (BMSCs) and chondrocytes (CCs) were obtained from the femoropatellar joints of young pigs, and seeded in agarose gel as a graft. During the 28-day culture, proliferation ability was measured by MTT assay, and gene expression of Collagen I, Collagen II, Aggrecan and SOX 9 were measured by qPCR. Qualitative and quantitative analysis of collagen, glycosaminoglycan and DNA were appraised by immunohistochemical staining and biochemical assay, and integration strength was analyzed by push-out tests.

Results: After 28-day culture, proliferation ability of CPCs and BMSCs was higher than CCs. Collagen, glycosaminoglycan, DNA content and chondrocyte-related genes expression in the cartilage progenitor cells seeded gel were significantly higher than the other two gels. Integration strength in the cartilage progenitor cells seeded gel was also higher compared with the other two gels.

Conclusion: Compared with CCs and BMSCs, CPCs in vitro have dominance in the ability of cell proliferation and differentiation as seed cells in tissue engineering.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/DDDT.S356936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059879PMC
May 2022

Low dose sacubitril/valsartan is effective and safe in hemodialysis patient with decompensated heart failure and hypotension: A case report.

Medicine (Baltimore) 2022 Apr 15;101(15):e29186. Epub 2022 Apr 15.

Nephrology Department, Sichuan Provincial People's Hospital, Chengdu, China.

Rationale: Severe heart failure in chronic hemodialysis (HD) patients is a great treatment challenge. Here we reported a chronic HD patient with the lowest ejection fraction reported so far and hypotension who well tolerated and benefited from angiotensin-receptor neprilysin inhibitor (ARNI) treatment.

Patient Concerns: This case was a 67 year old lady with decompensated heart failure and hypotension who was on regular HD. Intensified hemofiltration failed to improve her heart failure symptoms and was also retarded by hypotension.

Diagnosis: Chronic HD with decompensated heart failure.

Interventions: In addition to regular HD, low does sacubitril/valsartan was initiated and titrated from 12/13 mg to 24/26 mg twice daily.

Outcomes: Sacubitril/valsartan treatment was well tolerated and did not affect ultrafiltration during HD treatment. Transthoracic echocardiology at 3 months after initiation of ARNI treatment indicated significant improvement of both systolic and diastolic cardiac function. The patient has improved from New York Heart Association class 4 to class 2.

Lessons: Low does ARNI treatment could effectively improve cardiac function in HD patients with heart failure and hypotension. It was also safe and well tolerated.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000029186DOI Listing
April 2022

Reconstruction of the central slip insertion of the extensor tendon with the partial slip of the flexor digitorum superficialis tendon.

Arch Orthop Trauma Surg 2022 Apr 22. Epub 2022 Apr 22.

Department of Hand Surgery, Suzhou Ruihua Orthopaedic Hospital, Suzhou, 215104, Jiangsu, China.

Introduction: To introduce the surgical approach and clinical effect of transferring the partial slips of the flexor digitorum superficialis (FDS) tendon to reconstruct the insertion of the central slip of the extensor tendon (CSET) through an established bone tunnel (BT).

Materials And Methods: From April 2019 to March 2021, nine patients (six males and three females) with the CSET insertion rupture or defect were admitted to the institution and the CSET insertion was reconstructed with partial tendon slips on both sides of the FDS. The active range of motion of the interphalangeal joint of the affected finger was measured by a goniometer, the degree of pain was evaluated by visual analogue scale (VAS), and the grip strength of the affected limb was measured by an electronic hand dynamometer.

Results: The average postoperative follow-up was 12 months. No complications occurred. At the last follow-up, six of the patients were very satisfied and three were satisfied with their recovery.

Conclusion: The reconstruction of the CSET insertion by transferring the partial tendon slips of the FDS seem to be safe and feasible with minimal invasion to the donor tendon.

Type Of Study/level Of Evidence: Therapeutic IV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00402-022-04443-zDOI Listing
April 2022

The natural history of breast cancer: a chronological analysis of breast cancer progression using data from the SEER database.

Ann Transl Med 2022 Mar;10(6):365

Department of Breast Disease, Peking University People's Hospital, Beijing, China.

Background: Numerous mathematical models and retrospective analyses have been conducted to explore the natural progression of breast cancer, but there is no precise timeline as to how long it takes breast cancer to progress from stages 0 to IV. Studying the natural history of breast cancer requires the follow-up of a large cohort of patients who received no treatment following diagnosis for a long period, but this has become unpractical in the modern era. In this study, we quantified the natural progression of breast cancer using the Surveillance, Epidemiology, and End Results (SEER) database which was collected the clinical-pathological characteristics and survival data of untreated breast cancer patients.

Methods: We included 12,687 female patients who did not receive surgical treatment, radiotherapy, or chemotherapy, had complete information on tumor staging, and had only 1 primary tumor in their lifetime in our study as the untreated group. Overall survival (OS) was calculated from the date of the latest diagnosis of breast cancer to the date of death. Medium survival time (MST) was defined as the time at which 50% of the patients reached the endpoint. The medium survival time (MST) was calculated for each stage. The progression time was calculated by subtracting the MST of a higher stage from that of a lower stage, which also indicated "life loss". Estrogen receptor (ER) negative and positive patients were analyzed as two separate groups.

Results: For the ER positive patients, the median progression times from stages 0 to I, I to II, II to III, and III to IV were 5.2±1.2, 4.2±0.5, 2±0.3, and 0.9±0.2 years, respectively, which were similar to those of the ER negative patients of 5.4±2.9, 4.7±1.3, 2.5±0.7, and 1.1±0.4 years (P=0.49). The survival of stage IV patients was longer in ER positive patients than ER negative patients (2.7±0.1 0.9±0.2 year, P<0.001).

Conclusions: ER negative patients appear to have the same progression time as that of ER positive patients in the primary site, but had a much worse process in the metastasis site.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-22-918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011255PMC
March 2022

Gene expression trend changes in breast cancer populations over two decades: insights from The Cancer Genome Atlas database.

Hereditas 2022 Mar 22;159(1):18. Epub 2022 Mar 22.

Department of Breast Surgery, Peking University People's Hospital, Beijing, China.

Background: Breast cancer has remained the most common malignancy in women over the past two decades. As lifestyle and living environments have changed, alterations to the disease spectrum have inevitably occurred in this time. As molecular profiling has become a routine diagnostic and objective indicator of breast cancer etiology, we analyzed changes in gene expression in breast cancer populations over two decades using The Cancer Genome Atlas database.

Methods: We performed Heatmap and Venn diagram analyses to identify constantly up- and down-regulated genes in breast cancer patients of this cohort. We used Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to visualize associated functional pathways.

Results: We determined that three oncogenes, PD-L2, ETV5, and MTOR and 113 long intergenic non-coding RNAs (lincRNAs) were constantly up-regulated, whereas two oncogenes, BCR and GTF2I, one tumor suppression gene MEN1, and 30 lincRNAs were constantly down-regulated. Up-regulated genes were enriched in "focal adhesion" and "PI3K-Akt signaling" pathways, etc., and down-regulated genes were significantly enriched in "metabolic pathways" and "viral myocarditis". Eight up-regulated genes exhibited doubled or higher expression and the expression of three down-regulated genes was halved or lowered and correlated with long-term survival.

Conclusions: In this study, we found that gene expression and molecular pathway enrichments are constantly changing with time, importantly, some altered genes were associated with prognostics and are potential therapeutic targets, suggesting that the current molecular subtyping system must be updated to keep pace with this dynamic change.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s41065-022-00230-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8939184PMC
March 2022

hsa-miR-875-5p inhibits tumorigenesis and suppresses TGF-β signalling by targeting USF2 in gastric cancer.

J Transl Med 2022 03 7;20(1):115. Epub 2022 Mar 7.

Department of Gastroenterological Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250021, China.

Background: Gastric cancer (GC) is one of the most common malignancies, and an increasing number of studies have shown that its pathogenesis is regulated by various miRNAs. In this study, we investigated the role of miR-875-5p in GC.

Methods: The expression of miR-875-5p was detected in human GC specimens and cell lines by miRNA qRT-PCR. The effect of miR-875-5p on GC proliferation was determined by Cell Counting Kit-8 (CCK-8) proliferation and 5-ethynyl-2'-deoxyuridine (EdU) assays. Migration and invasion were examined by transwell migration and invasion assays as well as wound healing assays. The interaction between miR-875-5p and its target gene upstream stimulatory factor 2(USF2) was verified by dual luciferase reporter assays. The effects of miR-875-5p in vivo were studied in xenograft nude mouse models. Related proteins were detected by western blot.

Results: The results showed that miR-875-5p inhibited the proliferation, migration and invasion of GC cells in vitro and inhibited tumorigenesis in vivo. USF2 was proved to be a direct target of miR-875-5p. Knockdown of USF2 partially counteracted the effects of miR-875-5p inhibitor. Overexpression of miR-875-5p could inhibit proliferation, migration and invasion and suppress the TGF-β signalling pathway by downregulating USF2.

Conclusions: MiR-875-5p can inhibit the progression of GC by directly targeting USF2. And in the future, miR-875-5p is expected to be a potential target for GC diagnosis and treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12967-022-03253-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900418PMC
March 2022

Effect of S-1 Plus Oxaliplatin Compared With Fluorouracil, Leucovorin Plus Oxaliplatin as Perioperative Chemotherapy for Locally Advanced, Resectable Gastric Cancer: A Randomized Clinical Trial.

JAMA Netw Open 2022 Feb 1;5(2):e220426. Epub 2022 Feb 1.

Department of Gastrointestinal Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Importance: Perioperative chemotherapy is a potential treatment for locally advanced gastric cancer. However, the optimal chemotherapy regimen remains unknown.

Objective: To investigate the safety and efficacy of S-1 plus oxaliplatin (SOX) vs fluorouracil, leucovorin, and oxaliplatin (FOLFOX) as a perioperative chemotherapy regimen for patients with locally advanced gastric cancer.

Design, Setting, And Participants: In this phase 3, open-label, multicenter, randomized clinical trial, patients from 12 Chinese hospitals were enrolled between June 2011 and August 2016, with a last follow-up date of September 2019. The primary tumor was evaluated as either invading the serosa or the adjacent structures with or without metastatic lymph nodes, and with no evidence of distant metastases. Data were analyzed from December 2019 to June 2020.

Interventions: Patients were randomly assigned (1:1) to receive either 6 perioperative (2-4 preoperative and 2-4 postoperative) 3-week cycles of 130 mg/m2 oxaliplatin on day 1 and 80 to 120 mg/d S-1 orally daily for 2 weeks (SOX) or 130 mg/m2 oxaliplatin, 400 mg/m2 fluorouracil, 400 mg/m2 leucovorin, and 2400 mg/m2 fluorouracil as 46-hour infusion on day 1 (FOLFOX).

Main Outcomes And Measures: The primary end point was 3-year overall survival (OS). An absolute noninferiority margin of -8% was chosen.

Results: A total of 583 patients were enrolled; 293 were randomized to the SOX group and 290 were randomized to the FOLFOX group. Twelve patients (2.1%) refused preoperative chemotherapy (5 patients in the SOX group and 7 patients in the FOLFOX group), leaving a total of 288 patients in the SOX group (median [range] age, 61 [24 to 78] years; 197 men [68.4%]) and 283 patients in the FOLFOX group (median [range] age, 62 [24 to 80] years; 209 men [73.9%]) who received preoperative chemotherapy. The 3-year OS rate was 75.2% (95% CI, 70.3% to 80.5%) in the SOX group and 67.8% (95% CI, 62.5% to 73.5%) in the FOLFOX group. The absolute difference of 3-year OS rate between the 2 groups was 7.4% (95% CI, -0.1% to 14.9%), which was greater than the prespecified noninferiority margin (-8%) and showed the noninferiority of perioperative chemotherapy with SOX compared with FOLFOX.

Conclusions And Relevance: In this randomized clinical trial, SOX was noninferior to FOLFOX as perioperative chemotherapy for patients with locally advanced gastric cancer and could be recommended as an alternative treatment for these patients in Asia.

Trial Registration: ClinicalTrials.gov Identifier: NCT01364376.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1001/jamanetworkopen.2022.0426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886520PMC
February 2022

High-Performance Photodetectors Based on the 2D SiAs/SnS Heterojunction.

Nanomaterials (Basel) 2022 Jan 24;12(3). Epub 2022 Jan 24.

Tianjin Key Laboratory of Functional Crystal Materials, Institute of Functional Crystal, Tianjin University of Technology, Tianjin 300384, China.

Constructing 2D heterojunctions with high performance is the critical solution for the optoelectronic applications of 2D materials. This work reports on the studies on the preparation of high-quality van der Waals SiAs single crystals and high-performance photodetectors based on the 2D SiAs/SnS heterojunction. The crystals are grown using the chemical vapor transport (CVT) method and then the bulk crystals are exfoliated to a few layers. Raman spectroscopic characterization shows that the low wavenumber peaks from interlayer vibrations shift significantly along with SiAs' thickness. In addition, when van der Waals heterojunctions of -type SiAs/-type SnS are fabricated, under the source-drain voltage of -1 V-1 V, they exhibit prominent rectification characteristics, and the ratio of forwarding conduction current to reverse shutdown current is close to 10, showing a muted response of 1 A/W under excitation light of 550 nm. The light responsivity and external quantum efficiency are increased by 100 times those of SiAs photodetectors. Our experimental results enrich the research on the IVA-VA group -type layered semiconductors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano12030371DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840698PMC
January 2022

Effects of freeze-drying and spray-drying on the physical and chemical properties of Perinereis aibuhitensis hydrolysates: Sensory characteristics and antioxidant activities.

Food Chem 2022 Jul 4;382:132317. Epub 2022 Feb 4.

Marine Science Research Institute of Shandong Province, Qingdao, PR China; Shandong Key Laboratory of Disease Control in Mariculture, Qingdao, PR China.

This work was to investigate the impact of drying on the physical, chemical stability and character properties of P. aibuhitensis hydrolysate. Properties including amino acid composition, color stability, molecular weight distribution, powder morphology, etc. were compared between the freeze drying powder (FD) and spray drying powder (SD). They were fractionated with ultra filtration in antioxidant activities test. FD and SD contained amounts of amino acids and umami amino acids. SD exhibited the higher lightness and whiteness. SD had more compounds between 451 Da and 6511 Da. The surface morphology of FD was porous and flaky while SD was microsphere. SD had more volatile flavor substances and higher antioxidant activities on DPPH, hydroxyl, and superoxide radical-scavenging. In summary, results demonstrated that drying methods indeed affected the characteristics of hydrolysate, and the one prepared by spray drying method had the potential to be utilized for antioxidant food development and seafood seasoning.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2022.132317DOI Listing
July 2022

A Novel Humanized Anti-Interleukin-6 Antibody HZ0408b With Anti-Rheumatoid Arthritis Therapeutic Potential.

Front Immunol 2021 19;12:816646. Epub 2022 Jan 19.

Coriell Institute for Medical Research, Camden, NJ, United States.

Interleukin-6 (IL-6), a pleiotropic cytokine that regulates immune responses and inflammatory reactions, plays a pivotal role in the development of rheumatoid arthritis (RA). Blockade of IL-6 signaling with the monoclonal antibody (mAb) represents an important advancement in RA treatment. Although two IL-6 receptor antibodies are already available in the clinic, there is no mAb specifically targeting the human IL-6 to block IL-6 signaling for RA treatment. In this study, we have developed a novel humanized anti-IL-6 mAb HZ-0408b with potent binding and neutralizing activity to human IL-6. We demonstrated that HZ-0408b has a high species specificity and low cross-reactivity. Moreover, HZ-0408b showed a more potent inhibitory effect on IL-6 signaling than Siltuximab, an FDA-approved anti-IL-6 chimeric mAb. HZ-0408b is comparable to Olokizumab, a humanized mAb against IL-6 that is already in phase III studies. We observed that HZ-0408b is well tolerated at doses that can achieve therapeutic serum levels in cynomolgus monkey. Most importantly, we proved that HZ-0408b treatment significantly ameliorated joint swelling after the onset of arthritis and dramatically reduced plasma C-reactive protein (CRP) levels in a monkey collagen-induced arthritis (CIA) model. Collectively, our findings using non-human primates indicate that humanized anti-IL-6 mAb HZ-0408b has excellent safety and efficacy profiles for RA therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.816646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808405PMC
March 2022

The Measurement of the Surface Dose in Regular and Small Radiation Therapy Fields Using Cherenkov Imaging.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338211073432

School of Physics, 162798Xi'an Jiaotong University, Xi'an 710049, China.

The aim of this study is to measure the output factor (OF) and profile of surface dose in regular and small radiation therapy fields using Cherenkov imaging (CI). A medical linear accelerator (linac) was employed to generate radiation fields, including regular open photon field (ROPF), regular wedge photon field (RWPF), regular electron field (REF) and small photon field (SPF). The photon beams consisted of two filter modes including flattening filter (FF) and flattening filter free (FFF). All fields were delivered to a solid water phantom. Cherenkov light was captured using a charge-coupled device system during phantom irradiation. The OF and profile of surface dose measured by CI were compared with those determined by film measurement, ionization chamber measurement and treatment planning system calculation in order to examine the feasibility of measuring surface dose OF and profile using CI. The discrepancy between surface dose OF measured by CI and that determined by other methods is less than 6% in ROPFs with size less than 10 × 10 cm, REFs with size less than 10 × 10 cm, and SPFs except for 1 × 1 cm field. In the flat profile region, the discrepancy between surface dose profile measured by CI and that determined by other methods is less than 4% in REFs and less than 3% in ROPFs, RWPFs, and SPFs except for 1 × 1 cm2 field. The discrepancy of the surface dose profile is in compliance with the recommendation by IAEA TRS 430 reports. The discrepancy between field width measured by CI and that determined by film measurement is equal to or less than 2 mm, which is within the tolerance recommend by the guidelines of linac quality assurance in regular open FF photon fields, SPFs, and REFs with cone size of 10 × 10 cm in area. CI can be used to quantitatively measure the OF and profile of surface dose. It is feasible to use CI to measure the surface dose profile and field width in regular open FF photon fields and SPFs except for 1 × 1 cm field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/15330338211073432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819764PMC
March 2022

Role of sacubitril-valsartan in the prevention of atrial fibrillation occurrence in patients with heart failure: A systematic review and meta-analysis of randomized controlled trials.

PLoS One 2022 26;17(1):e0263131. Epub 2022 Jan 26.

Department of Cardiology, Yichang Hospital of Traditional Chinese Medicine, Yichang, China.

Objective: Heart failure (HF) and atrial fibrillation (AF) are often coexisting. They have common risk factors and pathophysiologic mechanisms. Sacubitril/valsartan has shown efficacy and tolerability in patients with HF. Thus, the study was performed to evaluate the impact of sacubitril/valsartan on AF occurrence in patients with HF.

Methods: The Embase and PubMed were searched from their inception date to June 2021 for all relevant randomized controlled trials (RCTs) evaluating the efficacy of sacubitril/valsartan in HF. The outcome of interest was the AF occurrence during the follow-up. Relative risks (RRs) with 95% confidence intervals (CIs) were pooled using a random-effects model.

Results: Six trials involving a total of 15,512 patients were included (7,750 randomized to sacubitril/valsartan and 7,762 to control). All trials were randomized, double-blind, and active-control. There was no significant difference in the prevention of AF occurrence between the sacubitril/valsartan group and the control group (enalapril or valsartan) in patients with HF (RR 1.07, 95%CI 0.95 to 1.19; I2 4%).

Conclusions: Sacubitril/valsartan was similar to either enalapril or valsartan in preventing the occurrence of AF in patients with HF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0263131PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8791471PMC
February 2022

Analysis of risk factors for early-onset ventilator-associated pneumonia in a neurosurgical intensive care unit.

BMC Infect Dis 2022 Jan 20;22(1):66. Epub 2022 Jan 20.

Department of Evidence-Based Medicine, Xuanwu Hospital Capital Medical University, Beijing, 100053, China.

Background: Ventilator-associated pneumonia (VAP) is a severe infection among patients in the neurosurgery intensive care unit (NICU).

Methods: We retrospectively evaluated risk factors for early-onset ventilator-associated pneumonia (EOVAP) from January 2019 to December 2019 at a NICU. A total of 89 NICU patients who were intubated within 48 h of onset and whose mechanical ventilation time was at least 7 days were enrolled. We evaluated EOVAP that occurred within the first 7 days after the onset of mechanical ventilation. The enrolled patients had no history of chronic lung disease and no clinical manifestations of infection before intubation. Clinical data of patients were recorded, and the incidence of and risk factors for EOVAP were analyzed. Patients were also grouped by age (≥ 65 vs. < 65 years) and whether they had received hypothermia treatment or not.

Results: Among 89 mechanically ventilated patients (49 men and 40 women; the mean age ± SD was 60.1 ± 14.3 years), 40 patients (44.9%) developed EOVAP within 7 days and 14 patients (15.7%) had a multidrug resistant bacterial infection. Binary logistic regression analysis indicated that older age (≥ 65 years) (odds ratio [OR]:3.53, 95% confidence interval [CI]:1.27-9.79, P = 0.015) and therapeutic hypothermia (OR:3.68, CI:1.10-12.31, p = 0.034) were independent predictors of EOVAP. Levels of peripheral blood leukocytes, neutrophils and platelets were lower in the therapeutic hypothermia group than those who did not receive hypothermia treatment.

Conclusions: This study found that older age (≥ 65 years) and therapeutic hypothermia were independently associated with the risk of EOVAP in NICU patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12879-022-07053-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772091PMC
January 2022

[Application of free anterolateral thigh flap with fascia lata for diabetic foot ulcers with bone exposure].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2022 Jan;36(1):86-91

Department of Plastic and Burn Surgery, Affiliated Hospital of Southwest Medical University, National Key Clinical Construction Specialty, Luzhou Sichuan, 646000, P. R. China.

Objective: To investigate the effectiveness of free anterolateral thigh flap (ALTF) with fascia lata in repairing diabetic foot ulcers (DFUs) with bone exposure.

Methods: Between January 2019 and January 2021, 20 patients with DFUs with bone exposure were admitted. There were 17 males and 3 females with a median age of 57.5 years (range, 48-76 years). There were 10 cases of Wagner grade 3 and 10 cases of grade 4. The DFUs formed 1 to 14 months, with a median time of 3 months. The patients underwent CT angiography, which showed extensive atherosclerosis in both lower limbs; 6 of them were severely narrowed or occluded and underwent percutaneous transluminal angioplasty. The size of wound ranged from 7 cm×6 cm to 27 cm×10 cm after applied first-stage debridement combined with vacuum sealing drainage treatment. In the second-stage, free ALTF with fascia lata was used to repair wounds and partial defects of tendons. The size of flap ranged from 8 cm×5 cm to 28 cm×11 cm. The wound of the donor site was sutured directly. The survival of the flap, the healing time of the wound, and the complications were recorded. The laser speckle blood flow imaging system was used to detect the blood perfusion of the flap and the skin around the flap at 2 weeks and 6 months after operation. The foot function was evaluated by American Orthopaedic Foot and Ankle Society (AOFAS) score at 6 months after operation.

Results: After operation, effusion under the flap happened in 6 cases, which cured after symptomatic treatment. Flaps survived completely in 14 cases. The tissue necrosis at the edges of the flaps occurred in 3 cases and healed after dressing changes. Venous crisis of flaps occurred in 3 cases, of which 1 case was completely necrotic after exploration, and the other 2 cases were partially alive. The wounds of 3 cases were repaired with skin grafts after debridement and dressing. The flap survival rate was 95.0%, and the limb salvage rate was 100%. The wound healing time after flap transplantation was 14-30 days, with an average of 19.1 days. Two patients had recurrence of peripheral skin ulcers of the flaps within 1 month after healing, which healed after conservative dressing changes. Eighteen cases of incisions at donor site healed by first intention, 2 cases had local skin necrosis and healed by debridement and suture. All patients were followed up 6-30 months, with a median time of 11 months. The texture, appearance, and elasticity of the flaps were good. All patients could walk alone without pain. At 6 months after operation, the AOFAS score was 75.9±11.9, which was significantly different from that (44.7±18.4) before operation ( =-7.025, =0.000). The blood perfusion value increased from (38.1±7.8) PU at 2 weeks to (42.7±10.3) PU, and the difference was significant ( =-4.680, =0.001).

Conclusion: Free ALTF with fascia lata has a rich blood supply and a high survival rate. It can be used to repair DFUs with bone exposure. After the free skin flap healed, it can promote revascularization of the affected foot, reduce the probability of ulcer recurrence, and avoid amputation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7507/1002-1892.202108110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844616PMC
January 2022

Electronic Properties and Carrier Dynamics at the Alloy Interfaces of WS Se Spiral Nanosheets.

Adv Mater 2022 Mar 4;34(11):e2107738. Epub 2022 Feb 4.

Tianjin Key Laboratory of Functional Crystal Materials, Institute of Functional Crystals, Tianjin University of Technology, Tianjin, 300384, China.

Electronic properties at the interfaces between different-composition domains of 2D-alloys are key for their optical, electronic, and optoelectronic applications. Understanding the interfacial electronic structures and carrier dynamics is essential for designing and fabricating devices that use these alloys. Here, WS Se spiral nanosheets are prepared using the physical vapor deposition method, and the nonlinear optical and electronic properties, as well as the carrier dynamics at the interfaces between the WS and WSe domains, are studied. Second-harmonic generation tests demonstrate that these nanosheets exhibit a very strong layer-dependent nonlinear optical effect. Atomic-resolution scanning tunneling microscopy (STM) and spectroscopy (STS) measurements reveal that S and Se atoms are non-uniformly distributed, forming WS domains, WSe domains, and defect-related areas. Atomic STM images and STS maps reveal enhanced local density of states by electron scattering at the WS/WSe interfaces, providing a detailed nanoscale interpretation of the S/Se-ratio-dependent lifetimes observed in pump-probe spectroscopy measurements. This work provides valuable interfacial characterization of 2D-alloy materials, using state-of-the-art methods with high temporal and spatial resolutions. The obtained insights are likely to be useful for prospective applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202107738DOI Listing
March 2022

Polarization-Independent Large Third-Order-Nonlinearity of Orthogonal Nanoantennas Coupled to an Epsilon-Near-Zero Material.

Nanomaterials (Basel) 2021 Dec 17;11(12). Epub 2021 Dec 17.

State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.

The nonlinear optical response of common materials is limited by bandwidth and energy consumption, which impedes practical application in all-optical signal processing, light detection, harmonic generation, etc. Additionally, the nonlinear performance is typically sensitive to polarization. To circumvent this constraint, we propose that orthogonal nanoantennas coupled to Al-doped zinc oxide (AZO) epsilon-near-zero (ENZ) material show a broadband (~1000 nm bandwidth) large optical nonlinearity simultaneously for two orthogonal polarization states. The absolute maximum value of the nonlinear refractive index n is 7.65 cm∙GW, which is 4 orders of magnitude larger than that of the bare AZO film and 7 orders of magnitude larger than that of silica. The coupled structure not only realizes polarization independence and strong nonlinearity, but also allows the sign of the nonlinear response to be flexibly tailored. It provides a promising platform for the realization of ultracompact, low-power, and highly nonlinear all-optical devices on the nanoscale.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano11123424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703912PMC
December 2021

Analysis of efficacy, safety and prognostic factors of DAC-HAA treatment in Chinese pediatric patients with refractory or relapsed acute myeloid leukemia.

Mol Clin Oncol 2021 Dec 1;15(6):269. Epub 2021 Nov 1.

Department of Pediatrics, The First Affiliated Hospital of University of Science and Technology of China, Hefei, Anhui 230001, P.R. China.

Hematopoietic stem cell transplantation (HSCT) is generally considered as the only effective treatment for children with relapsed/refractory (R/R) acute myeloid leukemia (AML). Achieving remission prior to HSCT affects the efficacy of the procedure and patient survival; therefore, induction therapy in children with R/R AML prior to HSCT is very important. The aim of the present study was to evaluate the clinical efficacy, prognosis and safety of 5-aza-2-deoxycytidine (DAC) combined with homoharringtonine + cytarabine + aclarubicin (HAA regimen) in the treatment of pediatric R/R AML. A total of 53 pediatric patients with R/R AML, aged 1-14 years, were treated with DAC-HAA. The overall response rate was 83.1%, with a complete remission rate of 77.4% and a partial remission rate of 5.7%. In conclusion, DAC-HAA therapy for children with R/R AML was found to be associated with a high remission rate, a short period of bone marrow suppression and a good safety profile. Therefore, DAC-HAA may be of value as a transitional regimen prior to HSCT and is worthy of clinical consideration.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mco.2021.2431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591692PMC
December 2021

Biological and Molecular Characterization of Two Closely Related Arepaviruses and Their Antagonistic Interaction in .

Front Microbiol 2021 18;12:755156. Epub 2021 Oct 18.

Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou, China.

Previously, our group characterized two closely related viruses from , areca palm necrotic ringspot virus (ANRSV) and areca palm necrotic spindle-spot virus (ANSSV). These two viruses share a distinct genomic organization of leader proteases and represent the only two species of the newly established genus of the family . The biological features of the two viruses are largely unknown. In this study, we investigated the pathological properties, functional compatibility of viral elements, and interspecies interactions in the model plant, . Using a newly obtained infectious clone of ANRSV, we showed that this virus induces more severe symptoms compared with ANSSV and that this is related to a rapid virus multiplication . A series of hybrid viruses were constructed the substitution of multiple elements in the ANRSV infectious clone with the counterparts of ANSSV. The replacement of either 5'-UTR-HCPro1-HCPro2 or CI effectively supported replication and systemic infection of ANRSV, whereas individual substitution of P3-7K, 9K-NIa, and NIb-CP-3'-UTR abolished viral infectivity. Finally, we demonstrated that ANRSV confers effective exclusion of ANSSV both in coinfection and super-infection assays. These results advance our understanding of fundamental aspects of these two distinct but closely related arepaviruses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.755156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558625PMC
October 2021

Near-infrared image recovery based on modulation instability in CdZnTe:V.

Opt Express 2021 Sep;29(20):31145-31155

We propose a near-infrared image recovery method based on modulation instability in the photorefractive semiconductor CdZnTe:V. The formation mechanism of modulation instability in CdZnTe:V is discussed, and the theoretical gain model is derived. Theoretical results of optical image recovery at 1 µm and 1.5 µm wavelengths demonstrate that the maximum cross-correlation gain is 2.6 with a signal to noise intensity ratio of 0.1. These results suggest that our method could be one of potential aids for near-infrared imaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.438061DOI Listing
September 2021

The addition of sawdust reduced the emission of nitrous oxide in pig manure composting by altering the bacterial community structure and functions.

Environ Sci Pollut Res Int 2022 Jan 14;29(3):3733-3742. Epub 2021 Aug 14.

Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.

Although composting, a measure to dispose agricultural waste, is widely accepted and applied, specific knowledge of microbially driven effects on nitrous oxide (NO) emissions during composting remains limited. Here, we monitored the impact of sawdust on NO emissions during pig manure composting. The results suggested that adding sawdust to the compost improved the compost temperature and reduced NO emissions. The addition of sawdust significantly altered the bacterial community structure and enhanced community turnover during the composting process. The addition of sawdust significantly reduced the relative abundance of denitrification and ureolysis, while increasing the relative abundance of nitrogen fixation. Specifically, adding sawdust may reduce NO emissions by reducing the relative abundance of Salinithrix, Truepera, Azomonas, Iamia, Silanimonas, Phycisphaera, and Gp21 during the thermophilic and mature phases of the composting period.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-15786-2DOI Listing
January 2022

Achieving enhanced biological phosphorus removal utilizing waste activated sludge as sole carbon source and simultaneous sludge reduction in sequencing batch reactor.

Sci Total Environ 2021 Dec 26;799:149291. Epub 2021 Jul 26.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.

Achieving enhanced biological phosphorus removal dominated by Tetrasphaera utilizing waste activated sludge (WAS) as carbon source could solve the problems of insufficient carbon source and excessive discharge of WAS in biological phosphorus removal. Up to now, the sludge reduction ability of Tetrasphaera remained largely unknown. Furthermore, the difference between traditional sludge fermentation and sludge fermentation dominated by Tetrasphaera was still unclear. In this study, two different sequencing batch reactors (SBRs) were operated. WAS from SBR-parent was utilized as sole carbon source to enrich Tetrasphaera with the relative abundance of 91.9% in SBR-Tetrasphaera. PO-P removal and sludge reduction could simultaneously be achieved. The effluent concentration of PO-P was 0, and the sludge reduction efficiency reached about 44.14% without pretreatment of sludge. Cell integrity detected by flow cytometry, the increase of DNA concentration in the sludge supernatant and decrease of particle size of activated sludge indicated that cell death and lysis occurred in sludge reduction dominated by Tetrasphaera. Stable structure of activated sludge was also damaged in this process, which led to the sludge reduction. By analyzing the excitation-emission matrix spectra of extracellular polymeric substances and the changes of carbohydrate and protein concentration, this study proved that slowly biodegradable organics (e.g., soluble microbial byproduct, tyrosine and tryptophan aromatic protein) could be better hydrolyzed and acidized to volatile fatty acids (VFAs) in sludge fermentation dominated by Tetrasphaera than traditional sludge fermentation, which provided carbon source for biological nutrients removal and saved operation cost in wastewater treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.149291DOI Listing
December 2021

Fabrication of an Anti-Reflective Microstructure on ZnS by Femtosecond Laser Bessel Beams.

Molecules 2021 Jul 14;26(14). Epub 2021 Jul 14.

State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics of CAS, Xi'an 710119, China.

As an important mid-infrared to far-infrared optical window, ZnS is extremely important to improve spectral transmission performance, especially in the military field. However, on account of the Fresnel reflection at the interface between the air and the high-strength substrate, surface optical loss occurs in the ZnS optical window. In this study, the concave antireflective sub-wavelength structures (ASS) on ZnS have been experimentally investigated to obtain high transmittance in the far-infrared spectral range from 6 μm to 10 μm. We proposed a simple method to fabricate microhole array ASS by femtosecond Bessel beam, which further increased the depth of the microholes and suppressed the thermal effects effectively, including the crack and recast layer of the microhole. The influence of different Gaussian and Bessel beam parameters on the microhole morphology were explored, and three ASS structures with different periods were prepared by the optimized Bessel parameters. Ultimately, the average transmittance of the sample with the ASS microhole array period of 2.6 μm increased by 4.1% in the 6 μm to 10 μm waveband, and the transmittance was increased by 5.7% at wavelength of 7.2 μm.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules26144278DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307567PMC
July 2021

Long non-coding RNA LINC00511 regulates the expression of microRNA-625-5p and activates signal transducers and activators of transcription 3 (STAT3) to accelerate the progression of gastric cancer.

Bioengineered 2021 12;12(1):2915-2927

Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China.

This study aimed to investigate the expression, biological function, and downstream mechanism of LINC00511 in gastric cancer (GC). In paired GC samples, LINC00511, miR-625-5p and STAT3 mRNA expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR); STAT3 protein expression was detected by immunohistochemical (IHC). The gain-of-function and loss-of-function models were established, and the proliferative and migrative ability of GC cells were measured by CCK-8 and transwell assays, respectively. The regulatory relationship between miR-625-5p and LINC00511 or STAT3 was examined by bioinformatics analysis, luciferase reporter gene assay, qRT-PCR, and western blot. We reported that LINC00511 and STAT3 expressions in GC tissues and cell lines were observably up-regulated, while miR-625-5p expression was inhibited. High expression of LINC00511 could facilitate the proliferation and promote the migration of GC cells. miR-625-5p was proved to be a downstream target of LINC00511, and LINC00511 could induce the expression of STAT3 by inhibiting the expression of miR-625-5p. Additionally, knockdown of LINC00511 suppressed the growth and lung metastases of CRC cells in nude mice. In conclusion, LINC00511 promotes the GC cell proliferation and migration via targeting the miR-625-5p/STAT3 axis, implying that LINC00511 can function as a target for GC therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2021.1940611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806821PMC
December 2021

Magneto-optically reorientation-induced image reconstruction in bulk nematic liquid crystals.

Opt Express 2021 May;29(11):17581-17590

We theoretically propose the magneto-optically reorientation-induced image reconstruction in bulk nematic liquid crystals (NLCs). The underlying signals are reinforced and recovered at the expense of scattering noise under reorientation-induced self-focusing nonlinearity. The intensity perturbation gain is derived and the numerical results are presented to show the response of NLC molecules to the diffusive images. The nonlinear image recovery is influenced by the input light intensity, the magnetic field direction, and the correlation length. The results suggest an alternative approach to detect noisy images and promote the application of NLCs in image processing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.425642DOI Listing
May 2021

Accuracy-enhanced coherent Ising machine using the quantum adiabatic theorem.

Opt Express 2021 Jun;29(12):18530-18539

The coherent Ising machine (CIM) implemented by degenerate optical parametric oscillator (DOPO) networks is a novel optical platform to accelerate computation of hard combinatorial optimization problems. Nevertheless, with the increase of the problem size, the probability of the machine being trapped by local minima increases exponentially. According to the quantum adiabatic theorem, a physical system will remain in its instantaneous ground state if the time-dependent Hamiltonian varies slowly enough. Here, we propose a method to help the machine partially avoid getting stuck in local minima by introducing quantum adiabatic evolution to the ground-state-search process of the CIM, which we call A-CIM. Numerical simulation results demonstrate that A-CIM can obtain improved solution accuracy in solving MAXCUT problems of vertices ranging from 10 to 2000 than CIM. The proposed machine that is based on quantum adiabatic theorem is expected to solve optimization problems more correctly.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.426476DOI Listing
June 2021

Developmental toxicity in marine medaka (Oryzias melastigma) embryos and larvae exposed to nickel.

Comp Biochem Physiol C Toxicol Pharmacol 2021 Oct 15;248:109082. Epub 2021 May 15.

Marine Science Research Institute of Shandong Province, QingDao, 266104, China. Electronic address:

As an important trace metal, nickel (Ni) has been reported extensively in the studies on freshwater animals. However, the toxic effects of Ni on marine organisms are not clearly understood. Therefore, in order to investigate the toxic effects of Ni on the early development of marine fish, the marine medaka (Oryzias melastigma) embryos and larvae were immersed in 0.13-65.80 mg/L Ni solution. The results showed that Ni exposure changed the egg size and heart rate of the embryos, lowered the hatchability, increased the deformity rate, and shortened the total body length of newly hatched larvae. Besides, it was found that before organogenesis and post-hatching periods were the sensitive periods of embryos to Ni. The 25 d LC value of embryos was 49.28 mg/L, and the 5 d LC of larvae was 55.92 mg/L, indicating that the embryos were more sensitive to Ni than the larvae. Furthermore, the expressions of the metallothionein (MT) gene, the skeletal development-related gene (Cyp26b1) and the cardiac development-related genes (ATPase, smyd1, cox2 and bmp4) were determined, and the results showed that the expressions of ATPase and smyd1 were up-regulated, while MT, Cyp26b1 and cox2 were significantly down-regulated at 9 days post-fertilization (dpf). Overall, Ni exposure caused a significant toxic effect on the early development of the O. melastigma embryos and larvae. Our findings could provide an important supplement to the toxicity data of tropical Ni and provide a reference for the exploration of the molecular mechanisms of Ni toxicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpc.2021.109082DOI Listing
October 2021

Pedicled vascularized versus non-vascularized bone grafts in the treatment of scaphoid non-union: a meta-analysis of comparative studies.

ANZ J Surg 2021 Nov 23;91(11):E682-E689. Epub 2021 Apr 23.

Department of Hand and Foot Surgery, Subei People's Hospital, Clinical Medical College, Yangzhou University, Yangzhou, China.

Background: Whether pedicled vascularized bone grafts (PVBGs) are beneficial over non-vascularized bone grafts (NVBGs) still remains controversial. The aim of this study was to compare the clinical results of PVBGs and NVBGs for the treatment of scaphoid non-union.

Methods: We conducted a meta-analysis of the published studies comparing outcomes of these two different surgical techniques for scaphoid non-union. Outcomes of union rate, time to union, functional results and re-operation rate were analysed.

Results: Seven studies including four randomized controlled studies and three retrospective comparative studies with 413 participants were identified fitting inclusion criteria. Meta-analysis showed that (i) union rate in PVBG groups was 1.13 times of NVBG groups (P = 0.002); (ii) the PVBG groups reached bone union significantly earlier by 1.73 weeks (P < 0.01); (iii) there was no significant difference in functional results, including active range of motion, grip strength, Mayo Wrist Score and excellent and good rate (P > 0.05); and (iv) re-operation rate was similar between the two groups (P = 0.65).

Conclusions: Although the PVBG technique attains higher union rate and earlier union, this radiological advantage does not bring any functional benefits. In addition, PVBGs are of greater technical difficulty and need more operation requirements. Hence, clinicians should be cautious in electing PVBGs for treating scaphoid non-union.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/ans.16894DOI Listing
November 2021

Application of the ACOSOG Z0011 criteria to Chinese patients with breast cancer: a prospective study.

World J Surg Oncol 2021 Apr 20;19(1):128. Epub 2021 Apr 20.

Breast Center, Peking University People's Hospital, Beijing, China.

Background: Although the ACOSOG Z0011 study showed that axillary lymph node dissection (ALND) could be avoided in a specific population of sentinel lymph node-positive patients, it is not widely accepted by Chinese surgeons. We conducted a prospective single-arm study to confirm whether or not the results of Z0011 are applicable to Chinese patients.

Methods: Patients conforming to the Z0011 criteria were prospectively enrolled at the Peking University People's Hospital Breast Center from November 2014 to June 2019. The clinicopathological features of the study group were compared with those of the Z0011 study group. Lymphedema after surgery, the incidence of local-regional recurrence, and survival were analyzed.

Results: One hundred forty-two patients who met the Z0011 eligibility criteria were enrolled in this study; 115 underwent sentinel lymph node biopsy (SLNB) alone. Compared with the Z0011 trial, younger patients were included (median age, 52 [26-82] years vs 54 [25-90] years; P = 0.03). For clinical T stage, tumor histology, hormone status, lymphovascular invasion, and the number of positive sentinel lymph nodes (SLNs), no statistically significant differences were observed. More patients received adjuvant chemotherapy and endocrine therapy in this study (90.85% vs 58.0% and 80.99% vs 46.6% respectively, P <0.001). A similar percentage of patients received radiotherapy, but more nodal radiotherapy procedures were carried out in our study (54.5% vs 16.9%). After a median follow-up of 29 months, only 1 patient (0.9%) had ipsilateral breast tumor recurrence, and no regional recurrence occurred.

Conclusion: Our study showed that it is achievable to avoid ALND in patients eligible for Z0011 in China.

Trial Registration: ClinicalTrials.gov. Registration number NCT03606616 . Retrospectively registered on 31 July 2018.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12957-021-02242-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059271PMC
April 2021

Soil microbiome manipulation triggers direct and possible indirect suppression against Ralstonia solanacearum and Fusarium oxysporum.

NPJ Biofilms Microbiomes 2021 04 12;7(1):33. Epub 2021 Apr 12.

Microbial Ecology Cluster, Genomics Research in Ecology and Evolution in Nature, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, the Netherlands.

Soil microbiome manipulation can potentially reduce the use of pesticides by improving the ability of soils to resist or recover from pathogen infestation, thus generating natural suppressiveness. We simulated disturbance through soil fumigation and investigated how the subsequent application of bio-organic and organic amendments reshapes the taxonomic and functional potential of the soil microbiome to suppress the pathogens Ralstonia solanacearum and Fusarium oxysporum in tomato monocultures. The use of organic amendment alone generated smaller shifts in bacterial and fungal community composition and no suppressiveness. Fumigation directly decreased F. oxysporum and induced drastic changes in the soil microbiome. This was further converted from a disease conducive to a suppressive soil microbiome due to the application of organic amendment, which affected the way the bacterial and fungal communities were reassembled. These direct and possibly indirect effects resulted in a highly efficient disease control rate, providing a promising strategy for the control of the diseases caused by multiple pathogens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41522-021-00204-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041757PMC
April 2021
-->