Publications by authors named "Hongjiao Li"

42 Publications

Research on electrostatic shielding characteristics of electrostatic precipitator.

J Air Waste Manag Assoc 2021 Dec 14. Epub 2021 Dec 14.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.

Air pollution control is a worldwide problem of common concern to all mankind. Electrostatic precipitator (ESP) plays an important role in the purification of flue gas and dust. At present, in a multi-electrode ESP, the middle discharge electrode is often inhibited by the outer discharge electrode, commonly known as electrostatic shielding, which seriously affects the electric field distribution and dust removal effect of ESP. In order to explore this characteristic, this research established a mathematical model of ESP and simulated the electrostatic shielding phenomenon of wire-plate ESP, which quantified the influence of various factors on the degree of electrostatic shielding and analyzed the potential impact of electrostatic shielding on particle charging and dust removal efficiency. The results of the study show that the wire-to-plate distance has the greatest impact on electrostatic shielding, followed by the discharge electrode radius, voltage, and the wire-to-wire distance. When the degree of electrostatic shielding increases, the dust removal efficiency of ESP decreases. In addition, increasing the number of discharge electrodes to increase the dust removal efficiency of ESP is only feasible within a certain range. When the number of discharge electrodes exceeds a certain threshold, the dust removal efficiency of ESP decreases due to the effect of electrostatic shielding. The results of the research provide guidance for the determination of the geometric parameters of ESP and can make theoretical predictions for the occurrence of electrostatic shielding, thereby effectively avoiding the occurrence of electrostatic shielding. Implications: Electrostatic precipitator plays an important role in the purification of flue gas and dust. Electrostatic shielding seriously affects the electric field distribution and dust removal effect of ESP. The research quantified the influence of various factors on the degree of electrostatic shielding and analyzed the potential impact of electrostatic shielding on particle charging and dust removal efficiency.. It is expected that through the research, some guidance will be provided for the determination of the geometric parameters of ESP and can make theoretical predictions for the occurrence of electrostatic shielding, thereby effectively avoiding the occurrence of electrostatic shielding.
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http://dx.doi.org/10.1080/10962247.2021.2017374DOI Listing
December 2021

Melatonin reduces proliferation and promotes apoptosis of bladder cancer cells by suppressing O-GlcNAcylation of cyclin-dependent-like kinase 5.

J Pineal Res 2021 Oct 18;71(3):e12765. Epub 2021 Sep 18.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, China.

Melatonin helps to maintain circadian rhythm, exerts anticancer activity, and plays key roles in regulation of glucose homeostasis and energy metabolism. Glycosylation, a form of metabolic flux from glucose or other monosaccharides, is a common post-translational modification. Dysregulated glycosylation, particularly O-GlcNAcylation, is often a biomarker of cancer cells. In this study, elevated O-GlcNAc level in bladder cancer was inhibited by melatonin treatment. Melatonin treatment inhibited proliferation and migration and enhanced apoptosis of bladder cancer cells. Proteomic analysis revealed reduction in cyclin-dependent-like kinase 5 (CDK5) expression by melatonin. O-GlcNAc modification determined the conformation of critical T-loop domain on CDK5 and further influenced the CDK5 stability. The mechanism whereby melatonin suppressed O-GlcNAc level was based on decreased glucose uptake and metabolic flux from glucose to UDP-GlcNAc, and consequent reduction in CDK5 expression. Melatonin treatment, inhibition of O-GlcNAcylation by OSMI-1, or mutation of key O-GlcNAc site strongly suppressed in vivo tumor growth. Our findings indicate that melatonin reduces proliferation and promotes apoptosis of bladder cancer cells by suppressing O-GlcNAcylation of CDK5.
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http://dx.doi.org/10.1111/jpi.12765DOI Listing
October 2021

Li-Ion Conductivity Enhancement of LiBH·NH with Formed LiO Nanoparticles.

ACS Appl Mater Interfaces 2021 Jul 28;13(27):31635-31641. Epub 2021 Jun 28.

Institute of New Energy and Low-Carbon Technology, Sichuan University, Chengdu 610207, China.

Interfacial engineering is an efficient approach to improve the ionic conductivity of solid-state electrolytes. In the present study, we report the enhancement of formed nanocrystalline LiO on the thermal stability and electrochemical properties of amide lithium borohydride, LiBH·NH ( = 0.67-0.8). LiBH·NH-LiO composites with different amounts of LiO are prepared by a one-step synthesis process by ball milling the mixture of LiBH, LiNH, and LiOH in molar ratios of 1:: ( = 1, 2, 3, 4). Owing to the strong interfacial effect with nanocrystalline LiO, LiBH·NH is converted to the amorphous state in the presence of 78 wt % LiO at = 4. Consequently, the ionic conductivity of LiBH·NH at 20 °C is improved by orders of magnitude up to 5.4 × 10 S cm, the NH desorption temperature is increased by more than 20 °C, and the electrochemical window is widened from 0.5 to 3.8 V.
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http://dx.doi.org/10.1021/acsami.1c06164DOI Listing
July 2021

Demalonylation of DDX3 by Sirtuin 5 promotes antiviral innate immune responses.

Theranostics 2021 24;11(15):7235-7246. Epub 2021 May 24.

Department of Anesthesiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

: Hosts defend against viral infection by sensing viral pathogen-associated molecular patterns and activating antiviral innate immunity through TBK1-IRF3 signaling. However, the underlying molecular mechanism remains unclear. : SiRNAs targeting Sirt1-7 were transfected into macrophages to screen the antiviral function. deficient mice or macrophages were subjected to viral infection to assess and function of Sirt5 by detecting cytokines, viral replicates and survival rate. Immunoprecipitation, WesternBlot and luciferase reporter assay were used to reveal molecular mechanism. : In this study, we functionally screened seven Sirtuin family members, and found that Sirtuin5 (Sirt5) promotes antiviral signaling and responses. deficiency leads to attenuated antiviral innate immunity and upon viral infection by decreasing TBK1-IRF3 activation and type I IFN production. Sirt5 overexpression increased antiviral innate immunity. Mechanism investigation revealed that Sirt5 interacts with DDX3 and demalonylates DDX3, which is critical for TBK1-IRF3 activation. Mutation of the demalonylation lysine sites (K66, K130, and K162) of DDX3 increased transcription. Furthermore, the acetylation on lysine 118 of DDX3 positively regulated transcription, whereas Sirt5 could not deacetylate this site. : Sirt5 promotes anti- RNA and DNA virus innate immune responses by increasing TBK1 signaling through demalonylating DDX3, which identifies a novel regulatory pathway of antiviral innate immune response.
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http://dx.doi.org/10.7150/thno.52934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210596PMC
July 2021

MiR-375 potentially enhances GnRH expression by targeting Sp1 in GT1-7 cells.

In Vitro Cell Dev Biol Anim 2021 Apr 26;57(4):438-447. Epub 2021 Mar 26.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 10021, People's Republic of China.

Gonadotropin-releasing hormone (GnRH) is the initial central regulator of the animal reproduction system, which is crucial for puberty onset and fertility. However, the mechanisms regulating GnRH production and release remain unclear. In addition, few studies reported that miR-375 expressed in mouse hypothalamus, but up to now there are limited functional studies of miR-375 in regulating GnRH secretion. According to our recent findings that miR-375 was involved in regulating the synthesis and secretion of pituitary hormones, thus, we aimed to identify the role of miR-375 in regulating GnRH production in GT1-7 cells. Immunofluorescence results demonstrated that miR-375 was expressed in all of the GT1-7 cells. The functional studies showed that miR-375 overexpression enhanced GnRH mRNA expression level, but decreased the mRNA expressions of Sp1, Cebpb, Msx1, and Tle4. Transcriptomics analysis demonstrated Sp1 and Tle4 acted as the targeting genes of miR-375, and Sp1 negatively regulated Gnrh mRNA expression by binding to the Gnrh promoter. Thus, we conclude that miR-375 potentially enhances GnRH expression by targeting Sp1 and Tle4 in GT1-7 cells. Our results highlight a critical role of miR-375 in regulating GnRH production, which may provide a novel potential therapeutic approach to neuroendocrine-disorder-related dysfunctions.
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http://dx.doi.org/10.1007/s11626-020-00447-4DOI Listing
April 2021

Design of synthetic microenvironments to promote the maturation of human pluripotent stem cell derived cardiomyocytes.

J Biomed Mater Res B Appl Biomater 2021 07 24;109(7):949-960. Epub 2020 Nov 24.

National Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Cardiomyocyte like cells derived from human pluripotent stem cells (hPSC-CMs) have a good application perspective in many fields such as disease modeling, drug screening and clinical treatment. However, these are severely hampered by the fact that hPSC-CMs are immature compared to adult human cardiomyocytes. Therefore, many approaches such as genetic manipulation, biochemical factors supplement, mechanical stress, electrical stimulation and three-dimensional culture have been developed to promote the maturation of hPSC-CMs. Recently, establishing in vitro synthetic artificial microenvironments based on the in vivo development program of cardiomyocytes has achieved much attention due to their inherent properties such as stiffness, plasticity, nanotopography and chemical functionality. In this review, the achievements and deficiency of reported synthetic microenvironments that mainly discussed comprehensive biological, chemical, and physical factors, as well as three-dimensional culture were mainly discussed, which have significance to improve the microenvironment design and accelerate the maturation of hPSC-CMs.
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http://dx.doi.org/10.1002/jbm.b.34759DOI Listing
July 2021

Effect of heat-clearing and detoxifying Chinese medicines combined with conventional therapy on mild hand, foot, and mouth disease with fever: An individual patient data meta-analysis.

Medicine (Baltimore) 2020 Jun;99(23):e20473

China Academy of Chinese Medical Sciences, Beijing, China.

Background: In China, heat-clearing and detoxifying Chinese medicines combined with conventional therapy are commonly applied to treat the mild hand, foot, and mouth disease (HFMD). However, there is lack of solid evidence on the efficacy and safety of such therapies.

Methods: We conducted a pooled analysis with individual patient data from 5 strictly randomized controlled clinical trials to assess the efficacy and safety of this combination therapy for mild HFMD. An intention-to-treat analysis was performed. A 2-stage meta-analysis method was adopted to analyze the pooled effect size.

Results: In total, 947 patients were included. Compared with conventional therapy, the combination therapy significantly reduced the progression rate of HFMD from mild to severe (odds ratio [OR] 0.43, 95% confidence interval [CI]: 0.22 to 0.83, P = .01). Meanwhile, the healing time of skin rash and oral ulcer in the combination therapy group was significantly shorter than that of conventional therapy. The overall hazard ratio (HR) of healing time of the skin rash or oral ulcer was 1.22 (95%CI: 1.04 to 1.43; P = .02). However, except Jinlianqingre effervescent tablets, the combination therapy cannot shorten the time to fever resolution (HR 1.12, 95%CI: 0.97 to 1.29, P = .14). Because of the heterogeneity, Jinlianqingre effervescent tablets were analyzed separately and the HRs of the time to fever resolution and the healing time of skin rash or oral ulcer were 3.88 (95%CI: 3.19 to 4.72; P < .0001) and 3.79 (95%CI: 2.81 to 5.11; P < .0001), respectively. There were 30 adverse events reported in total; 2 cases were related to Chinese medicines.

Conclusion: In conclusion, the heat-clearing and detoxifying Chinese medicines on top of conventional therapy can effectively reduce the progressive rate of mild HFMD and improve healing of skin and oral mucosal lesions. More studies are needed for the time to fever resolution.
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http://dx.doi.org/10.1097/MD.0000000000020473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306329PMC
June 2020

Insights into the effect of the shape of collecting plates on particle precipitation processes in an electrostatic precipitator.

J Air Waste Manag Assoc 2020 09 14;70(9):892-903. Epub 2020 Aug 14.

Zhejiang Feida Environmental Science and Technology Co, Ltd ., Zhuji, Zhejiang, People's Republic of China.

Collecting plate is the core component of the electrostatic precipitator. In order to explore the influences of the plates shape and electrode configuration on the particle precipitation process, numerical models of an ESP with seven different types of collecting plates were established and calculated. The simulation results showed that the shape of the collecting plate did have a great impact on the electric field, airflow particles movement. From the perspective of electric field characteristics, the space current density and electrostatic shielding effect in multielectrode ESPs varied with the shape of the plates under the same supply voltage. When using C-type plate, the shielding effect was minimal and the current density distribution of the plate was more uniform. When it comes to airflow distribution, small vortex structures were easily generated near the plates when the CW type, the rod-curtain and Z-type plates were used, leading to particle re-entrainments. In terms of collection efficiency, the C-type plate enjoyed the highest efficiency, while the ZT-type and corrugated plate were less efficient. The main source of air pollution is the flue gas released by fuel combustion. electrostatic precipitator plays an important role in pollutant discharge control and environmental protection. As the core component of the electrostatic precipitator, the form of the collecting plate exerts a serious impact on its dust removal performance. The study focuses on the analysis of the collecting plate shape on the electric field characteristics of electrostatic precipitators, flow distribution characteristics and the influence of the collecting efficiency. It is expected that through the research, some guidance will be provided with the electrode configuration selection and optimization of ESP.
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http://dx.doi.org/10.1080/10962247.2020.1768965DOI Listing
September 2020

Long non-coding RNA LINC00152 acts as a sponge of miRNA-193b-3p to promote tongue squamous cell carcinoma progression.

Oncol Lett 2020 Mar 13;19(3):2035-2042. Epub 2020 Jan 13.

Department of Stomatology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China.

Dysregulated expression of long non-coding RNAs has been determined to be important in cancer development; however, their role in tongue squamous cell carcinoma (TSCC) progression and carcinogenesis, to the best of our knowledge, is yet to be elucidated. The present study revealed that long intergenic non-coding RNA 00152 (LINC00152) expression was significantly increased in human TSCC tissues compared with in tissues from matched controls using RT-qPCR. In TSCC cell lines, CAL-27 and SCC-9, LINC00152 was revealed to promote TSCC cell proliferation, enhance cell cycle progression and inhibit cell apoptosis. Additionally, migration and invasion of TSCC cell lines was increased in response to LINC00152 overexpression. Mechanistically, LINC00152 was determined to be localized in the cytoplasm and acted as a microRNA (miR)-193b-3p sponge, and LINC00152 knockdown or miR-193b-3p mimics both inhibited PI3K signaling pathway activation and downstream AKT phosphorylation; therefore, promoting TSCC progression . Overall, the results of the present study suggested that increased LINC00152 expression in TSCC tissues may act as a sponge of miR-193b-3p to promote cancer progression .
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http://dx.doi.org/10.3892/ol.2020.11293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039063PMC
March 2020

Controlling cell viability and bacterial attachment through fabricating extracellular matrix-like micro/nanostructured surface on titanium implant.

Biomed Mater 2020 03 2;15(3):035002. Epub 2020 Mar 2.

School and Hospital of Stomatology, Lanzhou University, Lanzhou 730000, People's Republic of China.

Good osseointegration and gingival epithelial sealing play a key role in preventing peri-implantitis of dental implants. In addition to antibacterial qualities, the transmucosal surface of the implant is beneficial to the growth of fibroblasts and epithelial cells, while its body surface is suitable for the growth of osteoblasts and is resistant to epithelial cells and fibroblasts. In this study, both microgrooves mimicking the extracellular matrix (ECM) and titanium (Ti) dioxide nanotubes with different parameter settings were produced on Ti surfaces. The behavior of MG63 osteoblasts, L929 fibroblasts, SCC epithelial cells and Porphyromonas gingivalis on these decorated Ti surfaces was detected to quantify their performances in terms of osseointegration, biological sealing and antimicrobial ability. Via a scoring method based on these results, we concluded that 100-50-20-10-5 μm width grooves arranged in the horizontal direction at 2 μm depth were the priority for the design of the implant's transmucosal surface. By changing the depth to 3.6 μm and further decorating with 55 nm nanotubes, a best surface design for the implant body was acquired. Hierarchical ECM-like micro/nano patterns could provide novel designs for dental implants to achieve excellent gingival epithelial sealing and osseointegration, which would facilitate the clinical application of dental implants.
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http://dx.doi.org/10.1088/1748-605X/ab70eeDOI Listing
March 2020

miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary.

J Endocrinol 2020 03;244(3):459-471

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.

MicroRNA-7 (miR-7) is an important modulator of a plenty of gene expressions and the interrelated biological processes, highly expressed in porcine pituitary. Norepinephrine (NE), acting as an important neurotransmitter or/and a hormone secreted excessively under stress, affects the synthesis and secretion of various hormones, including pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which are the key hormones which regulate sexual maturation and reproductive functions. However, the relationship among NE, miR-7 and gonadotropin needs to be elucidated. The aim of this study was to identify whether miR-7 involved in the NE-adrenoceptor signaling pathway affects the synthesis and secretion of FSH and LH in porcine pituitary. Our results showed that the NE intracerebroventricular injection increased pituitary miR-7 level and the synthesis and secretion of FSH and LH in porcine, whereas the inhibition of either endogenous miR-7 or β-adrenergic receptors hindered the rise of FSH and LH synthesis induced by NE in cultured primary porcine anterior pituitary cells. Further, we identified the molecular type of β-adrenergic receptors and the signaling pathway in porcine pituitary, and we found that NE played its roles relying on adrenoceptor beta 2 (β2AR) and the RAF/MEK/ERK1/2 signaling pathway. The phosphorylation of ERK1/2 upregulated miR-7 level which subsequently enhanced FSH and LH synthesis by targeting to Golgi glycoprotein 1 (GLG1). These suggest that miR-7 mediates NE's effect on promoting FSH and LH synthesis in porcine pituitary.
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http://dx.doi.org/10.1530/JOE-19-0331DOI Listing
March 2020

Study on performance of electrostatic precipitator under multi-physics coupling.

Environ Sci Pollut Res Int 2019 Dec 30;26(34):35023-35033. Epub 2019 Oct 30.

School of Mechanical Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

A wire-plate electrostatic precipitator (ESP) is developed to analyze the particle transport characteristics and the influence of various factors on the performance of ESP. Above all, an experimental device is built to measure the current density distribution of the plates and obtain good consistency with the numerical simulation results, taking the ESP model established by COMSOL/Multiphysics as the numerical simulating object. Firstly, the electric field is solved by finite element method(FEM) to obtain the potential and charge density distribution. Then, the influence of secondary flow on the main flow at different flow velocities is explored. Finally, multi-physics coupling calculations show the influence of dust particle properties, electrode configuration, and operating conditions on ESP performance. The study found that the particle diameter is positively correlated with its charge, force, and motion, and the relative permittivity of the particles affects the collecting efficiency by affecting its charge difficulty. The wire-to-wire spacing is not proportional to collecting efficiency, when the spacing is 80 mm, the efficiency and the corona current can be maximized. Average electric field strength, corona current density, and current density distribution standard deviation satisfy the cubic function relationship. In addition, the effect of airflow velocity on collecting efficiency and particle precipitation is revealed. It provides a valuable basis for design and performance optimization of ESP.
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http://dx.doi.org/10.1007/s11356-019-06623-8DOI Listing
December 2019

Investigation of the optimal suspension culture time for the osteoblastic differentiation of human induced pluripotent stem cells using the embryoid body method.

Biochem Biophys Res Commun 2019 08 6;515(4):586-592. Epub 2019 Jun 6.

Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, 2 Anzhen Road, Chaoyang District, Beijing, 100029, China. Electronic address:

The differentiation of human induced pluripotent stem cells (hiPSCs) into osteoblasts provides a new paradigm in the field of bone tissue regeneration. The embryoid body (EB) differentiation method is commonly used for the osteogenic differentiation of hiPSCs. However, the spontaneous differentiation process of EBs is poorly understood, as evidenced by the inconsistency of the suspension time among previously reported studies as well as the low osteoblastic differentiation efficiency of hiPSCs. In the present study, we investigated the effects of the suspension culture time of EBs on the osteogenic differentiation of hiPSCs. Under chemically defined conditions, the expression of key genes related to presomitic mesoderm, neural crest, mesenchymal and pre-osteoblast cells in EBs derived from hiPSCs was examined daily by quantitative RT-PCR. Then, EBs with varying times in suspension (3, 5, 7 or 10 days) were attached onto gelatine surfaces, and their osteoblastic differentiation efficiencies after 14 days of culture in osteogenic induction medium were determined. Our results showed that EBs derived from hiPSCs subjected to 4 days of suspension culture produced the most mesenchymal stem cells, and exhibited the best osteogenic differentiation efficiency. Our research is valuable to standardizing, the time in suspension for the osteogenic differentiation of hiPSCs through the EB method, and facilitated the development of a high-efficiency in vitro osteogenic differentiation system for hiPSCs.
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http://dx.doi.org/10.1016/j.bbrc.2019.05.177DOI Listing
August 2019

Screening the optimal hierarchical micro/nano pattern design for the neck and body surface of titanium implants.

Colloids Surf B Biointerfaces 2019 Jun 21;178:515-524. Epub 2019 Mar 21.

Central Laboratory, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, 100081, PR China. Electronic address:

In this study, microgroove patterns and nanotubes with various parameter settings were hierarchically prepared on the titanium (Ti) surface, aiming to study the optimal hierarchical micro/nano pattern design for the neck and body surface of Ti dental implants. The adhesion and proliferation of MG63 osteoblasts, L929 fibroblasts, SCC epithelial cells and P. gingivalis on these Ti substrates were investigated to evaluate their performance in osseointegration, gingival epithelial sealing and antibacterial ability. Using a novel scoring method based on the cell or bacteria viability results, we found that 10 μm width and 2 μm depth groove pattern decorating with 55 nm diameter nanotubes was the best choice for the design of implant neck surface. Moreover, 20 μm width and 3.6 μm depth groove pattern decorating with 55 nm nanotubes was suitable for the implant body design. Our study systematically evaluated and screened the effects of different hierarchical micro/nano patterns on the growth of implant-healing related cells and bacteria. It preliminarily proved that it is feasible to reduce the incidence of peri-implantitis through the optimal hierarchical design of implants, which has great value in implant surface design and promoting the application of dental implants in clinical.
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http://dx.doi.org/10.1016/j.colsurfb.2019.03.045DOI Listing
June 2019

MicroRNA-7 inhibits melatonin synthesis by acting as a linking molecule between leptin and norepinephrine signaling pathways in pig pineal gland.

J Pineal Res 2019 Apr 25;66(3):e12552. Epub 2019 Feb 25.

College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China.

MicroRNAs, including microRNA-7 (miR-7), are important modulators of numerous gene expressions and the related biological processes. Melatonin is a key hormone regulating daily and seasonal rhythms, in which a variety of positive and negative regulatory factors, such as norepinephrine (NE) and leptin, are involved. However, the interactions among these factors and the mechanisms remain to be elucidated. The aims of the present study were to identify the functions and the related mechanisms of miR-7 in regulating melatonin synthesis and secretion through in vitro and in vivo experiments in pineal gland of pigs, which is an important animal model for agricultural and biomedical studies. Our results firstly show that miR-7 is specifically expressed in porcine pinealocytes and negatively regulates melatonin synthesis. The further functional studies show that the dynamic expression levels of miR-7 are contrary to the melatonin levels throughout the day, and the forced inhibition of endogenous miR-7 in porcine pinealocytes sharply increases arylalkylamine N-acetyltransferase (AANAT) expression by 80.0% (P = 0.0031) and melatonin levels by 81.0% (P = 0.0421), whereas miR-7 over-expression down-regulates AANAT expression by 38.6% (P = 0.0004) and melatonin levels by 37.6% (P = 0.0212). In addition, the miR-7 expression is up-regulated by leptin through the JAK/STAT3 signaling pathway, and the in vivo intracerebroventricular injection of leptin increases miR-7 expression by 80.0% (P = 0.0044) in porcine pineal glands and reduces melatonin levels by 57.1% (P = 0.0060) compared with the controls. This functional inhibition of melatonin synthesis by miR-7 is accomplished by its binding to the 3'-UTR of Raf1. Further, our results demonstrate that the RAF1/MEK/ERK signaling pathway mediates NE-induced AANAT expression, whereas leptin attenuates NE's function through miR-7. Taken together, the results demonstrated that leptin activates the JAK/STAT3 signaling pathway to increase the expression of miR-7, which acts as a negative regulatory molecule inhibiting NE-activated RAF1/MEK/ERK signaling pathway by targeting Raf1, resulting in decreased AANAT expression and melatonin synthesis. These findings suggest that miR-7 is a novel negative regulator of melatonin synthesis and links leptin- and NE-mediated signaling pathways in porcine pineal glands, which will contribute to our understanding in the establishment of the biological rhythms resulting from melatonin.
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http://dx.doi.org/10.1111/jpi.12552DOI Listing
April 2019

Isolation and characterization of organic matter-degrading bacteria from coking wastewater treatment plant.

Water Sci Technol 2018 Nov;78(7):1517-1524

College of Environmental & Resources Sciences, Shanxi University, 92 Wu-cheng Road, Taiyuan 030006, China E-mail:

As a step toward bioaugmentation of coking wastewater treatment 45 bacteria strains were isolated from the activated sludge of a coking wastewater treatment plant (WWTP). Three strains identified as Bacillus cereus, Pseudomonas synxantha, and Pseudomonas pseudoaligenes exhibited high dehydrogenase activity which indicates a strong ability to degrade organic matter. Subsequently all three strains showed high naphthalene degradation abilities. Naphthalene is a refractory compound often found in coking wastewater. For B. cereus and P. synxantha the maximum naphthalene removal rates were 60.4% and 79.8%, respectively, at an initial naphthalene concentration of 80 mg/L, temperature of 30 °C, pH of 7, a bacteria concentration of 15% (V/V), and shaking speed of 160 r/min. For P. pseudoaligenes, the maximum naphthalene removal rate was 77.4% under similar conditions but at 35 °C.
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http://dx.doi.org/10.2166/wst.2018.427DOI Listing
November 2018

Highly time-resolved aerosol characteristics during springtime in Weizhou Island.

J Environ Sci (China) 2018 Oct 20;72:64-74. Epub 2017 Dec 20.

Scientific Research Academy of Guangxi Environmental Protection, Nanning 530022, China.

A single particle aerosol mass spectrometer was deployed to measure the changes of single particle species and sizes during March 2015 in Weizhou Island of the Beibu Gulf, Guangxi province, South China. In this campaign, a total of 3,100,597 particles were sized, and 25.8% particles with both positive and negative mass spectrum were collected and 24.8% characterized in combination with the ART-2a neural network algorithm. The distribution of sized particles was mainly in from 520 to 600nm, and the diameters ranging from 340 to 1000nm accounted for above 90%. Eight types of particles were classified: Elemental Carbon containing (EC), Organic Carbon containing (OC), EC and OC combined containing particles, Na containing particles, K containing particles (K), Levoglucosan containing particles, mineral containing particles, and Heavy Metal containing particles (HM). EC, OC and K were the major containing particles, which accounted for 84.3% in the eight types particles. The relative ratio and size distribution of the three types were EC (48.1%, 620nm), OC (12.7%, 440nm), and K (23.5%, 600nm), respectively. The three types of particles were a bit increasing ratios compared with those in clean periods during haze pollution periods. Combined with the back-trajectory results from the Hysplit-4 model and local pollution sources revealed that the ambient air quality on the Weizhou Island may be influenced by biomass burning in the Indochina Peninsula (biomass burning in the Indochina Peninsula) from the transportation on higher level atmospheric layer and by mainland of south China located northeast of Weizhou Island on the ground.
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http://dx.doi.org/10.1016/j.jes.2017.12.012DOI Listing
October 2018

Multiple Roles of Glycans in Hematological Malignancies.

Front Oncol 2018 6;8:364. Epub 2018 Sep 6.

College of Life Science, Northwest University, Xi'an, China.

The three types of blood cells (red blood cells for carrying oxygen, white blood cells for immune protection, and platelets for wound clotting) arise from hematopoietic stem/progenitor cells in the adult bone marrow, and function in physiological regulation and communication with local microenvironments to maintain systemic homeostasis. Hematological malignancies are relatively uncommon malignant disorders derived from the two major blood cell lineages: myeloid (leukemia) and lymphoid (lymphoma). Malignant clones lose their regulatory mechanisms, resulting in production of a large number of dysfunctional cells and destruction of normal hematopoiesis. Glycans are one of the four major types of essential biological macromolecules, along with nucleic acids, proteins, and lipids. Major glycan subgroups are N-glycans, O-glycans, glycosaminoglycans, and glycosphingolipids. Aberrant expression of glycan structures, resulting from dysregulation of glycan-related genes, is associated with cancer development and progression in terms of cell signaling and communication, tumor cell dissociation and invasion, cell-matrix interactions, tumor angiogenesis, immune modulation, and metastasis formation. Aberrant glycan expression occurs in most hematological malignancies, notably acute myeloid leukemia, myeloproliferative neoplasms, and multiple myeloma, etc. Here, we review recent research advances regarding aberrant glycans, their related genes, and their roles in hematological malignancies. Our improved understanding of the mechanisms that underlie aberrant patterns of glycosylation will lead to development of novel, more effective therapeutic approaches targeted to hematological malignancies.
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http://dx.doi.org/10.3389/fonc.2018.00364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135871PMC
September 2018

Effect of inhibin A on proliferation of porcine granulosa cells in vitro.

Theriogenology 2018 Jul 26;114:136-142. Epub 2018 Mar 26.

College of Animal Sciences, Jilin University, Changchun 130062, China. Electronic address:

Inhibins regulate folliculogenesis, gametogenesis and hormone secretion via endocrine, paracrine and autocrine manners, and play roles in encouraging or suppressing proliferation of cells. In order to investigate the effects of inhibin A on proliferation and apoptosis of porcine granulosa cells (GCs), GCs were isolated from ovarian follicles (3-6 mm), treated with inhibin A at different concentrations. The cell viability, mitochondrial membrane potential (MMP), expression of proliferation-related genes and cell cycle were detected. Inhibin α subunit (INHA) gene was silenced to detect the effect of down-regulation of inhibin on expression of proliferation-related genes in GCs. The results showed that cell viability was associated with inhibin A concentration and significantly enhanced by inhibin A at high doses (50 and 100 ng/mL, P < 0.05) compared to control group (0 ng/mL). Meanwhile, the MMP boosted after treated with 100 ng/mL inhibin A for 48 h. Expression of proliferating cell nuclear antigen (PCNA) (200 ng/mL) and CyclinB1 (100 and 200 ng/mL) was promoted while Caspase-3(100 and 200 ng/mL) and BAX (200 ng/mL) was inhibited in dose dependent manner after the cells were incubated with inhibin A for 24 h (P < 0.05) compared to control group, thereby the transition from G1 phase to S phase was promoted and the number of S phase cells was increased. After silencing the INHA gene expression, expression of Caspase-3 was enhanced and CyclinB1 was inhibited (P < 0.05) compared to Ri-negative group. All the results pointed to the conclusion that inhibin A promotes the proliferation of GCs while inhibits apoptosis.
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http://dx.doi.org/10.1016/j.theriogenology.2018.03.034DOI Listing
July 2018

LIM homeobox transcription factor Isl1 is required for melatonin synthesis in the pig pineal gland.

J Pineal Res 2018 Aug 6;65(1):e12481. Epub 2018 Apr 6.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.

Melatonin is a key hormone that regulates circadian rhythms, metabolism, and reproduction. However, the mechanisms of melatonin synthesis and secretion have not been fully defined. The purpose of this study was to investigate the functions of the LIM homeobox transcription factor Isl1 in regulating melatonin synthesis and secretion in porcine pineal gland. We found that Isl1 is highly expressed in the melatonin-producing cells in the porcine pineal gland. Further functional studies demonstrate that Isl1 knockdown in cultured primary porcine pinealocytes results in the decline of melatonin and arylalkylamine N-acetyltransferase (AANAT) mRNA levels by 29.2% and 72.2%, respectively, whereas Isl1 overexpression raised by 1.3-fold and 2.7-fold. In addition, the enhancing effect of norepinephrine (NE) on melatonin synthesis was abolished by Isl1 knockdown. The in vivo intracerebroventricular NE injections upregulate Isl1 mRNA and protein levels by about threefold and 4.5-fold in the porcine pineal gland. We then examined the changes in Isl1 expression in the pineal gland and global melatonin levels throughout the day. The results show that Isl1 protein level at 24:00 is 2.5-fold higher than that at 12:00, which is parallel to melatonin levels. We further found that Isl1 increases the activity of AANAT promoter, and the effect of NE on Isl1 expression was blocked by an ERK inhibitor. Collectively, the results presented here demonstrate that Isl1 positively modulates melatonin synthesis by targeting AANAT, via the ERK signaling pathway of NE. These suggest that Isl1 plays important roles in maintaining the daily circadian rhythm.
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http://dx.doi.org/10.1111/jpi.12481DOI Listing
August 2018

[Analysis on the concepts related to adverse events and adverse reactions of acupuncture].

Zhongguo Zhen Jiu 2018 Jan;38(1):87-90

Institute of Basic Research of Clinical Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.

With the increasing popularity of acupuncture therapy in the world, the safety of acupuncture has attracted more attention. The objective and impartial assessment of the safety of acupuncture becomes very important. The "adverse events of acupuncture" and "adverse reactions of acupuncture" are the common concepts in the research of acupuncture safety. In the paper, on the basis of the conceptual characteristics of "adverse events" and "adverse reactions" and in combination with the particular characteristics of acupuncture itself, the connation and features of the concepts of the "adverse events of acupuncture" and "adverse reactions of acupuncture" are analyzed. The interaction and differences are summarized. Both "adverse events of acupuncture" and "adverse reactions of acupuncture" are in the category of adverse medical events. The "adverse events of acupuncture" includes the damages induced by acupuncture therapy and also those without relationship with acupuncture therapy. The "adverse reactions of acupuncture" refers to the adverse outcomes induced by acupuncture therapy specially. It has the definite relationship with acupuncture therapy. Hence, the application of "adverse reactions of acupuncture" is more objective and accurate in the assessment of acupuncture safety.
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http://dx.doi.org/10.13703/j.0255-2930.2018.01.023DOI Listing
January 2018

[Establishing the acupuncture-moxibustion clinical trial registry and improving the transparence of clinical trials of acupuncture and moxibustion].

Zhongguo Zhen Jiu 2017 Jul;37(7):685-689

China Academy of Chinese Medical Sciences, Beijing 100700.

As a kind of intervention measures of traditional Chinese medicine, acupuncture-moxibustion is highly adopted on global clinical practice. Even though the global clinical trial registration system was established more than 10 years ago, the proportion of acupuncture-moxibustion clinical trial registration is still very low; and it is very problematic on the methodological quality and report quality in the published acupuncture-moxibustion clinical trials. In order to manage particularly the acupuncture-moxibustion clinical trials, China Academy of Chinese Medical Sciences, collaborated with China Association of Acupuncture and Moxibustion and World Federation of Acupuncture Societies, established the Acupuncture-Moxibustion Clinical Trail Registry (AMCTR). AMCTR is a secondary registry platform affiliated to the Chinese Clinical Trial Registry (ChiCTR) and WHO International Clinical Trials Registry Platform (ICTRP), specifically for the acceptance and management of clinical trials in the field of acupuncture and moxibustion. It is a nonprofit academic organization, located in China Academy of Chinese Medical Sciences.
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http://dx.doi.org/10.13703/j.0255-2930.2017.07.001DOI Listing
July 2017

Association of gene polymorphisms of estrogen receptor, follicle-stimulating hormone β and leptin with follicular cysts in Large White sows.

Theriogenology 2017 Nov 7;103:143-148. Epub 2017 Apr 7.

College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, PR China. Electronic address:

Ovarian follicular cysts are one of leading causes of infertility and financial loss in pig breeding program. This study was carried out to investigate the association between polymorphisms of estrogen receptor (ESR), follicle-stimulating hormone (FSH) β, and leptin genes and follicular cysts in sows. A total of 47 and 120 sows with follicular cysts and normal follicles, respectively, were selected to evaluate whether these candidate loci affect the formation of follicular cysts in sows. The polymorphisms of ESR, FSHβ, FSHβ/HaeIII and leptin genes were tested by PCR and PCR-RFLP methods. Cyst-normal case data analysis showed that ESR/PvuII polymorphisms are highly associated with follicular cysts and that sows ESR/PvuII genotype have lower rate of suffering from cysts (P = 0.021). Unfortunately, FSHβ, FSHβ/HaeIII, and leptin C3469T polymorphisms were found no significant difference in follicular cysts sows and normal sows. These results suggest that FSHβ, FSHβ/HaeIII, and leptin C3469T genotypes are not able to effect the presence of follicular cysts (P > 0.05). In addition, the haplotype EBCM and EBTM within four loci of genes had significant dominance effect on follicular cysts (P < 0.05). The detection of ESR/PvuII polymorphisms and haplotype EBCM and EBTM can positively improve the development of biological biomarkers, which is thereby beneficial in breeding and ovary-protective therapy of reproductive disease in pigs.
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http://dx.doi.org/10.1016/j.theriogenology.2017.03.030DOI Listing
November 2017

Effect of Electroacupuncture on Urinary Leakage Among Women With Stress Urinary Incontinence: A Randomized Clinical Trial.

JAMA 2017 06;317(24):2493-2501

Guang'an Men Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Importance: Electroacupuncture involving the lumbosacral region may be effective for women with stress urinary incontinence (SUI), but evidence is limited.

Objective: To assess the effect of electroacupuncture vs sham electroacupuncture for women with SUI.

Design, Setting, And Participants: Multicenter, randomized clinical trial conducted at 12 hospitals in China and enrolling 504 women with SUI between October 2013 and May 2015, with data collection completed in December 2015.

Interventions: Participants were randomly assigned (1:1) to receive 18 sessions (over 6 weeks) of electroacupuncture involving the lumbosacral region (n = 252) or sham electroacupuncture (n = 252) with no skin penetration on sham acupoints.

Main Outcomes And Measures: The primary outcome was change from baseline to week 6 in the amount of urine leakage, measured by the 1-hour pad test. Secondary outcomes included mean 72-hour urinary incontinence episodes measured by a 72-hour bladder diary (72-hour incontinence episodes).

Results: Among the 504 randomized participants (mean [SD] age, 55.3 [8.4] years), 482 completed the study. Mean urine leakage at baseline was 18.4 g for the electroacupuncture group and 19.1 g for the sham electroacupuncture group. Mean 72-hour incontinence episodes were 7.9 for the electroacupuncture group and 7.7 for the sham electroacupuncture group. At week 6, the electroacupuncture group had greater decrease in mean urine leakage (-9.9 g) than the sham electroacupuncture group (-2.6 g) with a mean difference of 7.4 g (95% CI, 4.8 to 10.0; P < .001). During some time periods, the change in the mean 72-hour incontinence episodes from baseline was greater with electroacupuncture than sham electroacupuncture with between-group differences of 1.0 episode in weeks 1 to 6 (95% CI, 0.2-1.7; P = .01), 2.0 episodes in weeks 15 to 18 (95% CI, 1.3-2.7; P < .001), and 2.1 episodes in weeks 27 to 30 (95% CI, 1.3-2.8; P < .001). The incidence of treatment-related adverse events was 1.6% in the electroacupuncture group and 2.0% in the sham electroacupuncture group, and all events were classified as mild.

Conclusions And Relevance: Among women with stress urinary incontinence, treatment with electroacupuncture involving the lumbosacral region, compared with sham electroacupuncture, resulted in less urine leakage after 6 weeks. Further research is needed to understand long-term efficacy and the mechanism of action of this intervention.

Trial Registration: clinicaltrials.gov Identifier: NCT01784172.
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http://dx.doi.org/10.1001/jama.2017.7220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5815072PMC
June 2017

Long noncoding RNAs in head and neck cancer.

Oncotarget 2017 Feb;8(6):10726-10740

Department of Stomatology,ChanghaiHospital, Second Military Medical University, Shanghai, P. R. China.

Head and neck cancers (HNCs) include a series of malignant tumors arising in epithelial tissues, typically oral cancer, laryngeal cancer, nasopharynx cancer and thyroid cancer. HNCs are important contributors to cancer incidence and mortality, leading to approximately 225,100 new patients and 77,500 deaths in China every year. Determination of the mechanisms of HNC carcinogenesis and progression is an urgent priority in HNC treatment. Long noncoding RNAs (lncRNAs) are noncoding RNAs longer than 200 bps. lncRNAs have been reported to participate in a broad scope of biological processes, and lncRNA dysregulation leads to diverse human diseases, including cancer. In this review, we focus on lncRNAs that are dysregulated in HNCs, summarize the latest findings regarding the function and molecular mechanisms of lncRNAs in HNC carcinogenesis and progression, and discuss the clinical application of lncRNAs in HNC diagnosis, prognosis and therapy.
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http://dx.doi.org/10.18632/oncotarget.12960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354695PMC
February 2017

MicroRNA-16 Modulates Melatonin-Induced Cell Growth in the Mouse-Derived Spermatogonia Cell Line GC-1 spg Cells by Targeting Ccnd1.

Biol Reprod 2016 09 27;95(3):57. Epub 2016 Jul 27.

College of Animal Sciences, Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, Jilin, P.R. of China

Melatonin exerts a range of physiological effects. However, the functional significance of melatonin in spermatogenesis and the underlying mechanisms remain unclear. MicroRNAs (miRNAs) are essential in the initiation and progression of testicular development, including spermatogenesis. Thus far, limited information is known about the role of miRNAs in melatonin-mediated spermatogenesis. In this study, the expression levels of testicular miRNA machinery genes, namely, Dgcr8 and Xpo5, were significantly increased by melatonin. The miRNA expression profile was identified in the testes of melatonin-treated mice. Ten miRNAs were significantly up-regulated, and 15 miRNAs were down-regulated. Melatonin (25 μM) enhanced cell growth and reduced apoptosis in GC-1 spg cells. Among the down-regulated miRNAs, miR-16 expression was influenced by melatonin in GC-1 spg cells. The miR-16 mimics in GC-1 spg cells significantly suppressed cell growth and promoted cell apoptosis. Conversely, transfection of the miR-16 inhibitor significantly increased cell growth and decreased cell apoptosis. The protein expression level of CCND1 (Cyclin D1) in GC-1 spg cells was decreased by the miR-16 mimics and increased by knockdown of miR-16. Moreover, bioinformatics and reporter activity analyses showed that Ccnd1 was a potential target of miR-16. These results suggested that miR-16 may function as a novel regulator of testicular functions during melatonin stimulation by targeting Ccnd1.
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http://dx.doi.org/10.1095/biolreprod.115.138313DOI Listing
September 2016

Enhancement of immunogenic response and protection in model rats by CSTM nanoparticles anticaries DNA vaccine.

Nanomedicine (Lond) 2016 06 12;11(11):1407-16. Epub 2016 May 12.

School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.

Aim: To construct anticaries DNA vaccine and evaluate its ability to elicit mucosal and systemic immune responses in rats.

Materials & Methods: wapA fragment was cloned into pVAX1 plasmid to generate pVAX1-wapA. The pVAX1-wapA/trimethyl chitosan nanoparticles were prepared by complex coacervation method.

Results: Significantly higher specific IgG antibody titers were observed in rats immunized with nanoparticles compared with rats immunized with naked pVAX1-wapA. Anti-WapA IgA and IgG antibody levels after intranasal immunization were significantly higher than those following intramuscular delivery of nanoparticles or naked pVAX1-wapA. Furthermore, fewer enamel, slight dentin and dentin moderate lesions were observed in rats immunized with nanoparticles.

Conclusion: The results implicate WapA as an excellent candidate for anticaries vaccine development and nanoparticles as an effective delivery system.
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http://dx.doi.org/10.2217/nnm-2016-0012DOI Listing
June 2016

BDNF-induced expansion of cumulus-oocyte complexes in pigs was mediated by microRNA-205.

Theriogenology 2016 May 12;85(8):1476-82. Epub 2016 Jan 12.

Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin, P. R. of China. Electronic address:

The neurotrophin family of proteins is required for the survival and differentiation of the nervous system and is important to the development of reproductive tissues. The objectives of the present study were to detect the presence of the brain-derived neurotrophic factor (BDNF) and its tyrosine kinase receptor protein in cumulus-oocyte complexes in pigs and to explore the role of microRNAs in the BDNF-induced in vitro maturation of oocytes. We demonstrate that both BDNF and tyrosine kinase receptor protein are expressed in porcine cumulus oocyte complexes. BDNF supplementation promotes the in vitro maturation of porcine oocytes. MiRNA-205 is downregulated during the BDNF-induced maturation of oocytes. The overexpression of miRNA-205 in granulosa cells and reporter gene assay shows that the marker gene ptx3 for cumulus expansion is the putative target gene of miR-205. Our data provide evidence that the BDNF-induced maturation of oocytes in pigs may be mediated by miR-205 through the regulation of potential target gene, ptx3.
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http://dx.doi.org/10.1016/j.theriogenology.2016.01.004DOI Listing
May 2016

Differential Expression of microRNAs in the Ovaries from Letrozole-Induced Rat Model of Polycystic Ovary Syndrome.

DNA Cell Biol 2016 Apr 8;35(4):177-83. Epub 2016 Jan 8.

1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .

Polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine disorder. To understand the pathogenesis of PCOS, we established rat models of PCOS induced by letrozole and employed deep sequencing to screen the differential expression of microRNAs (miRNAs) in PCOS rats and control rats. We observed vaginal smear and detected ovarian pathological alteration and hormone level changes in PCOS rats. Deep sequencing showed that a total of 129 miRNAs were differentially expressed in the ovaries from letrozole-induced rat model compared with the control, including 49 miRNAs upregulated and 80 miRNAs downregulated. Furthermore, the differential expression of miR-201-5p, miR-34b-5p, miR-141-3p, and miR-200a-3p were confirmed by real-time polymerase chain reaction. Bioinformatic analysis revealed that these four miRNAs were predicted to target a large set of genes with different functions. Pathway analysis supported that the miRNAs regulate oocyte meiosis, mitogen-activated protein kinase (MAPK) signaling, phosphoinositide 3-kinase/Akt (PI3K-Akt) signaling, Rap1 signaling, and Notch signaling. These data indicate that miRNAs are differentially expressed in rat PCOS model and the differentially expressed miRNA are involved in the etiology and pathophysiology of PCOS. Our findings will help identify miRNAs as novel diagnostic markers and therapeutic targets for PCOS.
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http://dx.doi.org/10.1089/dna.2015.3145DOI Listing
April 2016
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