Publications by authors named "Honggang Xu"

18 Publications

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BMSC-Derived Exosomes Ameliorate Osteoarthritis by Inhibiting Pyroptosis of Cartilage via Delivering miR-326 Targeting HDAC3 and STAT1//NF-B p65 to Chondrocytes.

Authors:
Honggang Xu Bin Xu

Mediators Inflamm 2021 2;2021:9972805. Epub 2021 Nov 2.

Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei City, Anhui Province 230022, China.

Background: In the past decade, mesenchymal stem cells (MSCs) have been widely used for the treatment of osteoarthritis (OA), and noncoding RNAs in exosomes may play a major role.

Aim: The present study is aimed at exploring the effect and mechanism of miR-326 in exosomes secreted by bone marrow mesenchymal stem cells (BMSCs) on pyroptosis of cartilage and OA improvement.

Methods: Exosomes from BMSCs (BMSC-Exos) were isolated and identified to incubate with OA chondrocytes. Proliferation, migration, specific gene and miR-326 expression, and pyroptosis of chondrocytes were detected. BMSCs or chondrocytes were transfected with miR-326 mimics or inhibitors to investigate the effect of miR-326 in BMSC-Exos on pyroptosis of chondrocytes and the potential mechanism. Finally, a rat OA model was established to verify the effect and mechanism of miR-326 in BMSC-Exos on cartilage of pyroptosis.

Results: Incubation with BMSC-Exos could significantly improve the survival rate, migration ability, and chondrocyte-specific genes (COL2A1, SOX9, Agg, and Prg4) and miR-326 expression of OA chondrocytes and significantly inhibit pyroptosis of chondrocytes by downregulation of the levels of inflammatory cytokines, Caspase-1 activity, and pyroptosis-related proteins such as GSDMD, NLRP3, ASC, IL-1, and IL-18 ( < 0.01). PKH26 labeling confirmed the uptake of BMSC-Exos by chondrocytes. Incubation with exosomes extracted from BMSCs overexpressing miR-326 can significantly repress the pyroptosis of chondrocytes, while knockdown of miR-326 had the opposite effect ( < 0.01). The same result was also demonstrated by direct interference with the expression level of miR-326 in chondrocytes ( < 0.01). In addition, we found that the overexpression of miR-326 significantly inhibited the expression of HDAC3 and NF-B p65 and significantly promoted the expression of STAT1, acetylated STAT1, and acetylated NF-B p65 in chondrocytes ( < 0.01). The targeted relationship between miR-326 and HDAC3 was verified by dual-luciferase reporter assay. Animal experiments confirmed the mechanism by which miR-326 delivered by BMSC-Exos inhibits pyroptosis of cartilage by targeting HDAC3 and STAT1/NF-B p65 signaling pathway.

Conclusion: BMSC-Exos can deliver miR-326 to chondrocytes and cartilage and improve OA by targeting HDAC3 and STAT1//NF-B p65 to inhibit pyroptosis of chondrocytes and cartilage. Our findings provide a new mechanism for BMSC-Exos to treat OA.
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http://dx.doi.org/10.1155/2021/9972805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577926PMC
November 2021

Enhanced antitumor activity of inulin-capped Se nanoparticles synthesized using Jerusalem artichoke tubers.

Glycoconj J 2021 10 27;38(5):599-607. Epub 2021 Jul 27.

Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-Molecules, Anhui Provincial Engineering Laboratory for Screening and Re-Evaluationvaluation of Active Compounds of Herbal Medicines in Southern Anhui, Wannan Medical College, Wuhu, 241002, China.

An inulin polysaccharide with a molecular weight of ~ 2600 Da was derived from Jerusalem artichoke tubers and referred to as "JAP". Previous studies have shown that inulin can improve glucose tolerance and the liver lipid profile; however, its antitumor activity remains to be examined in detail. Therefore, to investigate the possible improvement of the antitumor activity of JAP, a novel nanostructured biomaterial was constructed by capping Se nanoparticles with JAP using sodium selenite, via a redox reaction with ascorbic acid, and referred to as "JAP-SeNPs". Transmission electron microscopy revealed that the average diameter of JAP-SeNPs is ~ 50 nm, and the C:Se mass ratio in JAP-SeNPs was found to be 15.4:1 by energy-dispersive X-ray spectroscopy. The well-dispersed JAP-SeNPs exhibited a significant in vitro antiproliferative effect on mouse forestomach carcinoma cells at a concentration of 400 μg/mL when incubated for 48 h, with an inhibition rate of 41.5%. Moreover, 38.9% of later apoptotic cells were observed. These results reveal that a combination of Se and JAP can effectively enhance the antitumor activity of polysaccharides obtained from Jerusalem artichoke tubers.
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http://dx.doi.org/10.1007/s10719-021-10011-1DOI Listing
October 2021

The Escalating Effects of Wildlife Tourism on Human-Wildlife Conflict.

Animals (Basel) 2021 May 12;11(5). Epub 2021 May 12.

School of Tourism Management, Sun Yat-sen University, Zhuhai 519082, China.

Human-wildlife conflict is a barrier to achieving sustainable biodiversity conservation and community development in protected areas. Tourism is often regarded as a tool to mitigate such conflict. However, existing studies have mainly adopted a socio-economic perspective to examine the benefits of tourism for communities, neglecting the ecological effects of tourism. This case study of macaque tourism on a peninsula in China illustrates that tourism can escalate rather than mitigate human-wildlife conflict. Fifty-three stakeholders were interviewed and secondary data were collected to understand the development of rhesus macaque () tourism and community-macaque conflict. The results show that food provision and tourist-macaque interactions rapidly increased the macaques' population, habituation, and aggressive behaviors, which led them to invade the surrounding community more often and exacerbated human-macaque conflict. Meanwhile, low community participation in tourism generated few benefits for residents and did not help alter residents' hostile attitudes towards the macaques. Local residents gradually retreated from agriculture as the macaques became more intrusive. A holistic approach to evaluating the role of wildlife tourism in resolving community-wildlife conflict is proposed and practical suggestions for alleviating such conflict are given.
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http://dx.doi.org/10.3390/ani11051378DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150641PMC
May 2021

Health tourism destinations as therapeutic landscapes: Understanding the health perceptions of senior seasonal migrants.

Soc Sci Med 2021 06 21;279:113951. Epub 2021 Apr 21.

School of Tourism Management, Sun Yat-sen University, Building 329, 135 Xingangxi Road, Guangzhou, 510275, PR China; Key Laboratory of the Sustainable Development of Xinjiang's Historical and Cultural Tourism, Xinjiang University, Urumqi, Xinjiang, 830046, PR China. Electronic address:

Health-driven seasonal tourism has become a new way of providing for the aged, which demonstrates a profound relationship between place and health. Therapeutic landscapes strongly suggest a causal relationship between health tourism destination and individual health. However, the quantitative validation of such relationship remains neglected and possible mechanisms underlying the relationship lack a strong theoretical basis. Based on therapeutic landscape theory and the REPLACE framework, this study quantitatively examines health promotion mechanisms of health tourism destinations by using data collected in Sanya, China, in January 2019. The effect of personal traits of dispositional optimism on health perceptions is also considered. The results show that physical, social, and symbolic landscapes positively influence health perceptions through restorative experiences. Social landscapes, symbolic landscapes and dispositional optimism positively affect health perception, while physical landscapes do not directly have a significant impact on health perception. It is expected that this study will fill the knowledge gap and advance our knowledge of therapeutic landscapes by exploring how they can influence health.
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http://dx.doi.org/10.1016/j.socscimed.2021.113951DOI Listing
June 2021

[Increased proportion and abnormal phenotype of NK cells in peripheral blood of patients with knee osteoarthritis].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2019 Dec;35(12):1115-1121

Department of Immunology, School of Basic Medicine, Anhui Medical University, Hefei 230022, China.

Objective To investigate the distribution and phenotypic characteristics of natural killer (NK) cells in the peripheral blood of knee osteoarthritis (KOA) patients. Methods Flow cytometry was used to detect and compare the distribution of NK cells and the expression of their surface functional receptors in the peripheral blood of healthy controls without KOA and patients with KOA, such as human leukocyte antigen DR (HLA-DR) and natural cytotoxicity receptor 3 (NCR3/NKP30). Results The proportion of NK cells in the peripheral blood of KOA patients was significantly up-regulated, especially CD16bright NK cells. The expression of HLA-DR molecules on the surface of NK cells with KOA was up-regulated, while the expression of NKP30 was significantly down-regulated. Conclusion The proportion of NK cells in the peripheral blood of KOA patients increases, and the cells show the up-regulated expression of CD16 and HLA-DR and the down-regulated expression of NKP30.
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December 2019

Carbon Nanoparticles Inhibit Α-Glucosidase Activity and Induce a Hypoglycemic Effect in Diabetic Mice.

Molecules 2019 Sep 6;24(18). Epub 2019 Sep 6.

Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Wuhu 241002, China.

New, improved therapies to reduce blood glucose are required for treating diabetes mellitus (DM). Here, we investigated the use of a new nanomaterial candidate for DM treatment, carbon nanoparticles (CNPs). CNPs were prepared by carbonization using a polysaccharide from L. root as the carbon source. The chemical structure and morphology of the CNPs were characterized using Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental analysis, and transmission electron microscopy. CNPs were spherical, 10-20 nm in size, consisting of C, H, O, and N, and featuring various functional groups, including C=O, C=C, C-O, and C-N. In vitro, the as-prepared CNPs could inhibit α-glucosidase with an IC value of 0.5677 mg/mL, which is close to that of the reference drug acarbose. Moreover, in vivo hypoglycemic assays revealed that the CNPs significantly reduced fasting blood-glucose levels in mice with diabetes induced by high-fat diet and streptozocin, lowering blood glucose after intragastric administration for 42 days. To the best of our knowledge, this is the first report of CNPs exhibiting α-glucosidase inhibition and a hypoglycemic effect in diabetic mice. These findings suggest the therapeutic potential of CNPs for diabetes.
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http://dx.doi.org/10.3390/molecules24183257DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767295PMC
September 2019

TGF-β1 promoted chondrocyte proliferation by regulating Sp1 through MSC-exosomes derived miR-135b.

Cell Cycle 2018 12 11;17(24). Epub 2018 Dec 11.

a Department of Sports trauma & Arthroscopy surgery , The 1st affiliated hospital of Anhui Medical University , Hefei , Anhui , People's Republic of China.

Objective: The aim of this study was to investigate the molecular mechanism of TGF-β1 in regulating chondrocyte proliferation through MSC-exosomes.

Methods: An osteoarthritis (OA) rat model was established. Cartilage degradation was quantified by using OARSI score. TGF-β1 was used to stimulate MSCs. The expressions of miR-135b and Sp1 in MSCs, MSC-exosomes and C5.18 cells were detected. The cell viability of C5.18 cells was measured using MTT assay.

Results: TGF-β1 stimulation enhanced miR-135b expression in MSC-exosomes, and MSC-exosomes derived miR-135b increased the cell viability of C5.18 cells. Moreover, miR-135b negatively regulated Sp1 expression. The cell viability of C5.18 cells in TGF-β1+miR-135b inhibitor+si-control group was reduced, while the cell viability in TGF-β1+miR-135b inhibitor+si-Sp1 group was enhanced. In rat experiments, OARSI score was decreased and the number of chondrocytes was increased in OA+TGF-β1+MSC-exosome group, while the score and the number had an opposite trend in OA+TGF-β1+MSC-miR135b inhibitor-exosome group.

Conclusion: TGF-β1 promoted chondrocyte proliferation by regulating Sp1 through MSC-exosomes derived miR-135b, then promoted cartilage repair.
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http://dx.doi.org/10.1080/15384101.2018.1556063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343719PMC
December 2018

Perceived Environmental, Individual and Social Factors of Long-Distance Collective Walking in Cities.

Int J Environ Res Public Health 2018 11 4;15(11). Epub 2018 Nov 4.

Shenzhen Tourism College, Jinan University, Shenzhen 518053, China.

Long-distance collective walking is a popular activity in cities across China. However, related research is limited, creating a research gap to explore participants' dynamic experience and related influential factors. Therapeutic mobilities theory explores the relationships among walking, health, and well-being from a qualitative perspective. Based on therapeutic mobilities theory, following a systematic process, this study develops a scale to quantitatively estimate the perceived environmental, personal, and social factors that may influence health and well-being. By applying construal level theory, this paper further hypothesizes that personality traits and familiarity moderate environmental, personal, and social perceptions. Data were collected with a paper survey ( = 926) from the "Shenzhen 100 km Walking" event. The findings highlight that long-distance collective walkers have comparatively greater experiences of health and well-being in three aspects: positive social interaction, individual development, and environmental understanding. Personality traits, familiarity, and gender moderate this well-being experience. Theoretical and managerial implications are discussed.
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http://dx.doi.org/10.3390/ijerph15112458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266780PMC
November 2018

Seasonal mobility and well-being of older people: The case of 'Snowbirds' to Sanya, China.

Health Place 2018 11 27;54:155-163. Epub 2018 Sep 27.

Department of Geography and Geographic Information Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address:

Seasonal retired migrants have increased rapidly in developing countries in recent years. This article adopts a relational perspective to understand the relationships between older people's seasonal mobility and well-being. It shows that the complex spatiotemporal dynamics of seasonal mobility and the non-western sociocultural context influence older people's relations with their places of origin and destination, which in turn shape their well-being experiences during seasonal mobility. Narrative analysis of interview data from five pairs of 'snowbirds' to Sanya, China, reveals that seasonal mobility and well-being of older people are evolving processes, interwoven with risks and opportunities. Older people construct routinized daily activities, stable social relations, and peer-supporting and active-aging environments in their place of destination to maintain short-term well-being. However, they encounter difficulties in integrating the corporeal and social dimensions of their bodily experiences and constructing long-term well-being due to their separations from their lifelong relations in their places of origin. But overall, seasonal mobility creates alternative options for aging across multiple places and promotes leisure-oriented aging life.
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http://dx.doi.org/10.1016/j.healthplace.2018.08.008DOI Listing
November 2018

Desert as therapeutic space: Cultural interpretation of embodied experience in sand therapy in Xinjiang, China.

Health Place 2018 09 23;53:173-181. Epub 2018 Aug 23.

School of Tourism Management, Sun Yat-Sen University, 329 Building, 135 West Xingang Road, Haizhu District, Guangzhou 510275, PR China. Electronic address:

The existing research on therapeutic landscapes reveals more positive and pleasant experiences generated in blue and green spaces and their effects on health. This study draws on a case study of sand therapy at Turpan City in China in order to explore in the 'yellow' space of the desert how painful embodied experiences and cultural beliefs are assembled to produce therapeutic experiences. The results show that the sand therapy participants sought painful haptic sensations such as burning, heat and sweating by touching hot sand as treatment. Individuals interpreted these painful bodily sensations through health-related cultural beliefs of yin-yang balance and Qi to generate particular therapeutic experiences. This study suggests the researchers to be more attentive to painful therapeutic landscapes.
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http://dx.doi.org/10.1016/j.healthplace.2018.08.005DOI Listing
September 2018

Therapeutic landscapes and longevity: Wellness tourism in Bama.

Soc Sci Med 2018 01 28;197:24-32. Epub 2017 Nov 28.

School of Tourism Management, Sun Yat-sen University, Guangzhou 510275, China. Electronic address:

Due to the rising demand for healthcare products and concern over environmental pollution, wellness tourism has been booming in China in recent years. The therapeutic landscape theory provides a multi-scale interpretation of wellness tourism to explore how wellness tourists achieve health in healing places. By presenting the results of 83 interviews conducted in Bama, China, this study reveals that the "longevity village" Bama, endorsed by centenarians, provides a retreat that combines natural beauty and a harmonious neighbourhood for wellness tourists. This article argues that although three themes-natural environment, social interaction and symbolic landscape-work together in the healing process of tourists, the symbolic landscape, which is significantly shaped by the longevity culture, plays a dominant role. Longevity in Chinese culture symbolizes the alignment of a strong body, graceful mind, and pleasant habitat. Furthermore, tourism reinforces the importance of symbols and imagination (of a place), the perception of longevity demonstrates the symbolic landscape and thus increases tourists' attachment to the place, and the unusual environment leads to a different therapeutic landscape from that of daily life. Finally, since to date there has been very few works on therapeutic landscapes in China, it is expected that this study will fill the knowledge gap and broaden the scope of application as well as conceptualization of the therapeutic landscape.
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http://dx.doi.org/10.1016/j.socscimed.2017.11.052DOI Listing
January 2018

Long-term outcomes after arthroscopic single-bundle reconstruction of the posterior cruciate ligament: A 7-year follow-up study.

J Int Med Res 2018 Feb 27;46(2):865-872. Epub 2017 Sep 27.

Department of Sport Injury & Arthroscopy, The First Affiliated Hospital of Anhui Medical University, Anhui, China.

Objective Arthroscopy is the most popular technique in posterior cruciate ligament (PCL) reconstruction surgery. However, no consensus on long-term outcomes after arthroscopic PCL reconstruction has been reached. This study was performed to evaluate the long-term outcomes after arthroscopic autologous hamstring or allogeneic tendon single-bundle reconstruction of the PCL. Methods Fifty-eight patients who underwent arthroscopic PCL reconstruction in Anhui, China from 2007 to 2009 were included. The follow-up period ranged from 56 to 83 months. During the follow-up, the Lysholm knee score and Tegner activity score were used to assess knee function. The KT-2000 arthrometer (MEDmetric Corp., San Diego, CA, USA) was used to assess the stability of the reconstructed PCL. Results The mean Lysholm score, mean Tegner score, and mean forward and backward displacements were not significantly different between the final follow-up and 1 year after the surgery. Additionally, no significant differences were observed in any of the above-mentioned parameters between autologous and allogeneic reconstruction at the final follow-up. Conclusion Both autologous and allogeneic reconstruction had few complications and satisfactory long-term outcomes.
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http://dx.doi.org/10.1177/0300060517722243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5971503PMC
February 2018

Initial Assessment and Implications for Surgery: The Missed Diagnosis of Irreducible Knee Dislocation.

J Knee Surg 2018 Mar 1;31(3):254-263. Epub 2017 May 1.

Department of Sports Medicine and Arthroscopic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Irreducible knee dislocation is a rare but devastating orthopedic emergency. Limited discussion about its characteristics has been undertaken due to its low incidence. The purpose of this study was to present a series of irreducible dislocated knees and cumulatively reviewed all existing publications in this filed. A retrospective case series study was undertaken in patients with irreducible knee dislocation. Patients' data were carefully collected and presented. Historical cases of irreducible knee dislocation in published papers were reviewed, and their diagnosis, treatment, and prognosis were summarized. Six patients with six irreducible knee dislocations were enrolled with an average age of 51.2 ± 9.7 years. Patterns of injuries were classified into KD-III M (three cases), KD-IV (two cases), and KD-V (one case). Dimple sign was presented in all cases on both physical examination and MRI. All patients received single-stage arthrotomy together with cruciate ligament reconstruction acutely. In cumulative literature review, 34 papers with 45 irreducible knee dislocations were included. KD-III M was the most familiar type of ligamentous injury (75.0%). Dimple sign was recorded in 83.7% occasions and the most frequent two trapped structures were medial retinaculum (31.8%) and MCL (43.1%). Open reduction was conducted in all cases to reduce the knee, and the prognosis of 88.0% cases was considered to be acceptable after different staged surgery. The "dimple" sign is pathognomonic but not necessary for diagnosis of irreducible knee dislocations. The general consensus for treatment is immediate neurovascular status assessment and acute open reduction.
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http://dx.doi.org/10.1055/s-0037-1602135DOI Listing
March 2018

Understanding seasonal mobilities, health and wellbeing to Sanya, China.

Soc Sci Med 2017 03 27;177:87-99. Epub 2017 Jan 27.

School of Business, Tourism Mobilities, Edinburgh Napier University, Craiglockhart Campus, Edinburgh, United Kingdom.

Both the ageing of the Chinese population and elderly mobility impact on the Chinese social infrastructure, triggering challenges to maintain elderly wellbeing. This paper reflects on the notion that seasonal mobility promotes wellbeing, and explores how two crucial factors, namely, forced migration and health conditions, influence the relations between seasonal retirement mobility and wellbeing. This study analyses amenity-led seasonal retired mobilities to Sanya as a case study, and adopts and develops a conceptual framework for relations between mobility and wellbeing in terms of daily activity, sociality, and context through seasonal mobility. Qualitative methods including participant observation, non-participant observation, in-depth interviews, and mobile ethnography were used to collect data. This revealed the heterogeneity of health conditions, and the constrained mobilities of seasonal retirees. Health and willingness for mobility are shown as significant factors in influencing the relations between mobility and wellbeing, which are in turn complicated and dynamic. Seasonal mobilities bring about difficulties for retirees particularly in terms of their efforts to reconstruct their previous life and self-continuities. However, it is argued that these retirees can merely maintain temporary and superficial wellbeing due to constant health concerns and uncertainties over potential temporary or permanent return to their places of origin. Those with serious health problems have more limitations, sacrificing other aspects of wellbeing for physical health. Practical implications from state, destination, and individual levels to better facilitate seasonal mobility and promote wellbeing are provided.
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http://dx.doi.org/10.1016/j.socscimed.2017.01.051DOI Listing
March 2017

An irreducible posterolateral knee dislocation: diagnosis, arthroscopic view, and treatment.

Am J Emerg Med 2016 Nov 17;34(11):2256.e3-2256.e4. Epub 2016 May 17.

Department of Sports Medicine and Arthroscopic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China. Electronic address:

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http://dx.doi.org/10.1016/j.ajem.2016.05.037DOI Listing
November 2016

Molecular cloning, sequence identification and expression analysis of novel caprine MYLPF gene.

Mol Biol Rep 2013 Mar 1;40(3):2565-72. Epub 2013 Jan 1.

Institute of Animal Genetics and Breeding Research, College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Yucity district, Yaan, 625014, Sichuan, China.

Skeletal muscle genes are important potentially functional candidate genes for livestock production and meat quality. Myosin regulatory light chain (MLC) regulates myofilament activation via phosphorylation by Ca(2+) dependent myosin light chain kinase. The cDNA of the myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF) gene from the longissimus dorsi of Tianfu goat was cloned and sequenced. The results showed that MYLPF full-length coding sequence consists of 513 bp and encodes 170 amino acids with a molecular mass of 19.0 kD. Two EF-hand superfamily domain of MYLPF gene conserved between caprine and other animals. The deduced amino acid sequence of MYLPF shared significant identity with the MYLPF from other mammals. A phylogenetic tree analysis revealed that the caprine MYLPF protein has a close genetic relationship and evolutional distance with MYLPF in other mammals. Analysis by RT-PCR showed that the MYLPF mRNA was detected in heart, liver, spleen, lung, kidney, gastrocnemius, abdominal muscle and longissimus dorsi. In particular, high expression levels of MYLPF mRNA were detected in the longissimus dorsi, gastrocnemius and abdominal muscle, and low level of expressions were observed in liver, spleen, lung and kidney. In addition, the temporal expression analysis further showed MYLPF expression decreased gradually with age in the skeletal muscle. This may be important as muscle growth occurs mainly in young age in goats. Western blotting results detected the MYLPF protein in four of the tissues in which MYLPF was shown to be expressed; the four exceptions were liver, spleen, lung and kidney.
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http://dx.doi.org/10.1007/s11033-012-2342-0DOI Listing
March 2013

Molecular cloning and tissue distribution of the phosphotyrosine interaction domain containing 1 (PID1) gene in Tianfu goat.

Gene 2013 Feb 10;515(1):71-7. Epub 2012 Dec 10.

Research Institution of Animal Genetics and Breeding, College of Animal Science and Technology of Sichuan Agricultural University, #46 Xinkang Road, Yucity District, Yaan 625014, Sichuan Province, China.

Phosphotyrosine interaction domain containing 1 (PID1) is an important mediator in the development of obesity-related insulin resistance in humans and animals. For a better understanding of the structure and function of the PID1 gene and to study its effect in caprine, the cDNA of the PID1 gene from the abdominal muscle of Tianfu goat was cloned and sequenced. The structure of PID1 was analyzed using bioinformatics tools. The results showed that the full sequence of the caprine PID1 cDNA was 896 bp long and contained a 654 bp long coding region that encoded a 217 amino acid sequence. Fifteen phosphorylation sites were predicted in the translated PID1 protein. The protein had a phosphotyrosine-binding domain between Arg(53) and Ile(199). A phylogenic tree based on the PID1 proteins from other species revealed that the caprine protein was closely related to cattle PID1. Fluorescence quantitative PCR analyses revealed that PID1 was expressed in the heart, liver, spleen, lung, kidney, leg muscle, abdominal muscle and longissimus dorsi muscle of goats. In particular, high expression levels of PID1 were detected in liver and abdominal muscle, and low expression levels were seen in lung. Furthermore, the PID1 mRNA expression levels in the longissimus dorsi muscles increased gradually with the age of the goats (P<0.05). Western blotting results detected the PID1 protein in six of the tissues in which PID1 was shown to be expressed; the two exceptions were liver and spleen.
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http://dx.doi.org/10.1016/j.gene.2012.11.025DOI Listing
February 2013

[Application of radiofrequency vaporization in arthroscopic treatment of patellofemoral joint disorders].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2009 Jan;23(1):60-3

Department of Orthopaedics, First Affiliated Hospital of Anhui Medical University, Hefei Anhui, 230022, P.R. China.

Objective: To explore the treatment of patellofemoral joint disorders with radiofrequency vaporization under arthroscopy.

Methods: From June 2004 to June 2007, 86 cases of patellofemoral joint disorder (98 knees) were treated by lateral retinacular release or medial retinacular tighten added, and combined with bipolar radiofrequency chondroplasty under arthroscopy. There were 30 males (34 knees) and 56 females (64 knees), aging 15-68 years (mean 40 years). The locations were left in 42 cases, right in 32 cases, both sides in 12 cases. The disease course was 3 months to 6 years (mean 30.5 months). In 98 knees, there were 28 knees of static patellar tilt, 10 knees of static patellar subluxation, 45 knees of dynamic patellar tilt and 15 knees of dynamic patellar subluxation. The Lysholm knee score preoperatively was 57.72 +/- 8.86.

Results: The patellofemoral cartilage abnormality were classified according to the Outerbridge grade (18 knees of grade I, 36 knees of grade II, 32 knees of grade III and 12 knees of grade IV). The incisions healed by first intention without complications such as infection or hemarthrosis. Seventy-five cases (82 knees) were followed up 8-37 months (mean 20.6 months). One month after operation, the patellofemoral joint pain was relieved remarkably, the knee joint activity was good and the radiological outcomes was also satisfactory. Six months after operation, the Lysholm knee score was improved significantly in patients of grades I, II and III (P < 0.05), while there was no significant change in patients of grade IV (P > 0.05). The postoperative score was 69.95 +/- 5.42 (P < 0.05).

Conclusion: Radiofrequency vaporization chondroplasty and soft tissue balance under arthroscopy are advantageous in terms of easy operation, less injury, slight reaction and have good effect on patellofemoral joint disorders.
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January 2009
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