Publications by authors named "Hongchuan Tian"

2 Publications

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Gallium (Ga)-strontium (Sr) layered double hydroxide composite coating on titanium substrates for enhanced osteogenic and antibacterial abilities.

J Biomed Mater Res A 2021 Jul 29. Epub 2021 Jul 29.

Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Bacterial infection and poor osteogenic capacity can result in the loosing or failure of titanium (Ti)-based implants in the clinic. Therefore, it is urgent to design an effective approach to enhance the osteogenic property and restrict bacterial activity. In this study, a layered double hydroxide (LDH) composed of Ga and Sr ions on Ti substrates by a hydrothermal method, then calcined in 250°C and denoted as LDH250. The scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) were confirmed that the LDH films were successfully formed on the Ti substrates. Importantly, the obtained LDH films can induce an alkaline microenvironment around the Ti surface and regulate the behaviors of osteogenic cells and bacteria. In vitro cellular experiments, the LDH250 can enhance the differentiation of both MC3T3-E1 cells and osteoblasts, stimulate alkaline phosphatase activity (ALP), collagen secretion, and mineralization levels. Meanwhile, antimicrobial assay against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) demonstrated that the LDH250 samples had strong antibacterial abilities, which attributed to the release profile of Ga could act as a "Trojan horse" to destroy the bacterial iron metabolism, inducing of local alkaline environment, and producing reactive oxygen species. Hence, this study provides an effective method for reducing antibacterial infection and enhancing the bone integrative capacity of Ti-based implants for orthopedic applications.
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July 2021

A novel biocomposite scaffold with antibacterial potential and the ability to promote bone repair.

J Biomater Appl 2021 Sep 17;36(3):474-480. Epub 2021 Feb 17.

Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, Yuzhong, People's Republic of China.

Clinical treatment of bone defects caused by trauma, tumor resection and other bone diseases, especially bone defects that can lead to infection, remains a major challenge. Currently, autologous bone implantation is the gold standard for treatment of bone defects, but it is limited by secondary trauma and insufficient autologous material. Moreover, postoperative infection is an important factor affecting bone healing.AcN-RADARADARADARADA-CONH2 (RADA) is a new type of self-assembling peptide(SAP) composed of Arg,Ala,Asp and other amino acids was designed and prepared. The "RADA" self-assembling peptide hydrogels has excellent biological activity and it's completely biodegradable and non-toxic.It is also have been confirmed to promote cell proliferation, wound healing, tissue repair, and drug delivery. To promote bone regeneration and simultaneously prevent bacterial infection, we designed biocomposite scaffolds comprising RADA and calcium phosphate cement (CPC), termed RADA-CPC. The morphological features of the scaffold were characterized by scanning electron microscopy (SEM). In vitro studies demonstrated that RADA-CPC enhances osteoblast proliferation, differentiation and mineralization. In addition, the scaffold was used as a drug delivery system to treat postoperative infections by sustained release of ciprofloxacin (CIP). The RADA-CPC scaffold may have potential application prospects in orthopedics field because of its role in promoting bone repair and as a sustained-release drug carrier to prevent infections.
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September 2021