Publications by authors named "Hongbo Zhao"

143 Publications

An optimized initialization for LDPC decoding over GF(q) in impulsive noise environments.

PLoS One 2021 6;16(5):e0250930. Epub 2021 May 6.

School of Electronic and Information Engineering, Beihang University, Beijing, P. R. China.

Modern navigation satellite communication has the characteristic of high transmitting rate. To avoid bit errors in data transmission, low density parity check (LDPC) codes are widely recognized as efficient ways for navigation communication. Conventionally, the LDPC decoding is applied for additive white Gaussian noise (AWGN) channel and degrades severely while facing the impulsive noise. However, navigation communication often suffers from impulsive interference due to the occurrence of high amplitude "spikes". At this time, the conventional Gaussian noise assumption is inadequate. The impulsive component of interference has been found to be significant which influences the reliability of transmitted information. Therefore the LDPC decoding algorithms for AWGN channel are not suitable for impulsive noise environments. Consider that LDPC codes over GF(q) perform better than binary LDPC in resisting burst errors for current navigation system, it is necessary to conduct research on LDPC codes over GF(q). In this paper, an optimized initialization by calculating posterior probabilities of received symbols is proposed for non-binary LDPC decoding on additive white Class A noise (AWAN) channel. To verify the performance of the proposed initialization, extensive experiments are performed in terms of convergence, validity, and robustness. Preliminary results demonstrate that the decoding algorithm with the optimized initialization for non-binary LDPC codes performs better than the competing methods and that of binary LDPC codes on AWAN channel.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250930PLOS
May 2021

c-Abl Tyrosine Kinase-Mediated Neuronal Apoptosis in Subarachnoid Hemorrhage by Modulating the LRP-1-Dependent Akt/GSK3β Survival Pathway.

J Mol Neurosci 2021 Mar 30. Epub 2021 Mar 30.

Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, People's Republic of China.

Accumulating evidence suggests that neuronal apoptosis plays a critical role in early brain injury (EBI) after subarachnoid hemorrhage (SAH), and the inhibition of apoptosis can induce neuroprotective effects in SAH animal models. c-Abl has been reported to promote neuronal apoptosis in Alzheimer's disease and cerebral ischemia, but its role in SAH had not been illuminated until now. In the present study, the effect of c-Abl on neuronal apoptosis induced by SAH was investigated. c-Abl protein levels and neuronal apoptosis were markedly increased 24 h after SAH, and the inhibition of endogenous c-Abl reduced neuronal apoptosis and mortality and ameliorated neurological deficits. Furthermore, c-Abl inhibition decreased the expression of cleaved caspase-3 (CC-3) after SAH. These results demonstrate the proapoptotic effect of c-Abl in EBI after SAH. Additionally, c-Abl inhibition further enhanced the SAH-induced phosphorylation of Akt and glycogen synthase kinase (GSK)3β. LY294002 abrogated the beneficial effects of targeting c-Abl and exacerbated neuronal apoptosis after SAH. SAH decreased LRP-1 levels and downregulated LRP-1 by RAP, and LRP-1 small interfering RNA (siRNA) induced a dramatic decrease in Akt/GSK3β activation in the presence of c-Abl siRNA. This is the first report showing that the c-Abl tyrosine kinase may play a key role in SAH-induced neuronal apoptosis by regulating the LRP-1-dependent Akt/GSK3β survival pathway. Thus, c-Abl has the potential to be a novel target for EBI therapy after SAH.
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http://dx.doi.org/10.1007/s12031-021-01835-5DOI Listing
March 2021

Active destruction of pyrite passivation by ozone oxidation of a biotic leaching system.

Chemosphere 2021 Mar 19;277:130335. Epub 2021 Mar 19.

School of Minerals Processing & Bioengineering, Central South University, Changsha, Hunan, China; Key Lab of Biohydrometallurgy of Ministry of Education, Changsha, Hunan, China.

Although pyrite bio-dissolution plays an important role in the processing of sulfide ores, the formation of passivation film inhibited the further dissolution of sulfide ores. In order to enhance the dissolution of sulfide ores, a novel method for destroying the passivation film using ozone was proposed and verified. The generated passivation film inhibiting pyrite dissolution in the presence of Leptospirillum ferrooxidans and Acidithiobacillus thiooxidans was studied. The X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) results indicate that a passivation film mainly consisting of jarosite and polysulfide (S/S) might be formed during biotic stage, which can be eliminated with the introduction of ozone (2 g/min) in 30 min. Electrochemical results show that ozone significantly increased the electrochemical reactivity of passivated pyrite, further proving that ozone enhanced the dissolution of passivated pyrite through destroying the passivation layer. Hence, a bi-stage method for dissolution of sulfide ores can be proposed.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130335DOI Listing
March 2021

Correlated Ion Transport and the Gel Phase in Room Temperature Ionic Liquids.

J Phys Chem B 2021 03 9;125(10):2677-2689. Epub 2021 Mar 9.

Department of Chemistry, Imperial College of London, Molecular Sciences Research Hub, White City Campus, Wood Lane, London W12 0BZ, U.K.

Here we present a theory of ion aggregation and gelation of room temperature ionic liquids (RTILs). Based on it, we investigate the effect of ion aggregation on correlated ion transport-ionic conductivity and transference numbers-obtaining closed-form expressions for these quantities. The theory depends on the maximum number of associations a cation and anion can form and the strength of their association. To validate the presented theory, we perform molecular dynamics simulations on several RTILs and a range of temperatures for one RTIL. The simulations indicate the formation of large clusters, even percolating through the system under certain circumstances, thus forming a gel, with the theory accurately describing the obtained cluster distributions in all cases. However, based on the strength and lifetime of associations in the simulated RTILs, we expect free ions to dominate ionic conductivity despite the presence of clusters, and we do not expect the percolating cluster to trigger structural arrest in the RTIL.
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http://dx.doi.org/10.1021/acs.jpcb.0c09050DOI Listing
March 2021

Fictitious phase separation in Li layered oxides driven by electro-autocatalysis.

Nat Mater 2021 Mar 8. Epub 2021 Mar 8.

Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.

Layered oxides widely used as lithium-ion battery electrodes are designed to be cycled under conditions that avoid phase transitions. Although the desired single-phase composition ranges are well established near equilibrium, operando diffraction studies on many-particle porous electrodes have suggested phase separation during delithiation. Notably, the separation is not always observed, and never during lithiation. These anomalies have been attributed to irreversible processes during the first delithiation or reversible concentration-dependent diffusion. However, these explanations are not consistent with all experimental observations such as rate and path dependencies and particle-by-particle lithium concentration changes. Here, we show that the apparent phase separation is a dynamical artefact occurring in a many-particle system driven by autocatalytic electrochemical reactions, that is, an interfacial exchange current that increases with the extent of delithiation. We experimentally validate this population-dynamics model using the single-phase material Li(NiMnCo)O (0.5 < x < 1) and demonstrate generality with other transition-metal compositions. Operando diffraction and nanoscale oxidation-state mapping unambiguously prove that this fictitious phase separation is a repeatable non-equilibrium effect. We quantitatively confirm the theory with multiple-datastream-driven model extraction. More generally, our study experimentally demonstrates the control of ensemble stability by electro-autocatalysis, highlighting the importance of population dynamics in battery electrodes (even non-phase-separating ones).
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http://dx.doi.org/10.1038/s41563-021-00936-1DOI Listing
March 2021

LncRNA LINC00857 strengthens the malignancy behaviors of pancreatic adenocarcinoma cells by serving as a competing endogenous RNA for miR-340-5p to upregulate TGFA expression.

PLoS One 2021 4;16(3):e0247817. Epub 2021 Mar 4.

Department of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, P. R. China.

Background: Pancreatic adenocarcinoma (PAAD) is a pancreatic disease with a high mortality rate in the world. This present research intends to identify the function of lncRNA LINC00857/miR-340-5p/Transforming growth factor alpha (TGFA) in the progression of PAAD.

Methods: Bioinformatics analysis was used to explore the differentially expressed lncRNA/miRNA/mRNA and analyze the relationship between lncRNA/miRNA/mRNA expression and prognosis of PAAD by enquiring TCGA, GEO and GTEX. KEGG pathway analysis and GO enrichment analysis were implemented to annotate the crucial genes regulated by LINC00857. The biological behaviors of PAAD cells were detected by CCK-8, colony formation and transwell assays. Interactive associations between LINC00857 and miR-340-5p, as well as miR-340-5p and TGFA were analyzed by dual luciferase assay.

Results: By enquiring TCGA database, we got that LINC00857 was highly expressed in patients with PAAD and positively associated with worse prognosis in PAAD patients. Moreover, LINC00857 upregulation promoted the proliferation and clone formation abilities of PAAD cells. Afterwards, the downstream miRNA and mRNA targets of LINC00857 were picked up to construct a ceRNA network. Further study revealed that TGFA expression was positively regulated by LINC00857 and negatively regulated by miR-340-5p. Besides that, the inhibitory effect of miR-340-5p on PAAD cells growth and movement can be blocked by LINC00857 upregulation. While, the malignant behavior of PAAD cells induced by TGFA overexpression can be eliminated by LINC00857 knockdown.

Conclusions: Upregulation of LINC00857 improved growth, invasion and migration abilities of PAAD cells by modulation of miR-340-5p/TGFA, affording potential targets and biomarkers for the clinical diagnosis and treatment.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247817PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932076PMC
March 2021

PLAC8 promotes the autophagic activity and improves the growth priority of human trophoblast cells.

FASEB J 2021 Mar;35(3):e21351

Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China.

Autophagy plays an important role in the normal development and function of trophoblast cells and is precisely regulated during pregnancy. Dysregulated autophagy contributes to the abnormal proliferation of trophoblasts, which is closely related to the occurrence of pregnancy-related diseases. Placenta specific 8 (PLAC8, Onzin) is a multifaceted protein proven to promote autophagy and potentiate various tumor progression. Its role in trophoblasts remains elusive. In our present study, PLAC8 expression was detected in tissues of first-trimester placentas (n = 5), term placentas (n = 5), choriocarcinoma (n = 5), and placental site trophoblastic tumor (n = 5). PLAC8 expression was increased in gestational neoplasms compared with normal pregnancies. mCherry-EGFP-LC3B reporter and transmission electron microscopy confirmed PLAC8 promoted the autophagic flux of human trophoblast cells. Both gain-of-function and loss-of-function experiments demonstrated PLAC8-regulated autophagy-related genes, including ATG5, ATG12, and Beclin-1. In addition, our data showed that PLAC8 co-localized with p53 and promoted its degradation, and p53 re-expression partially abrogated the PLAC8-induced autophagy activity. Furthermore, the overexpression of PLAC8 promoted cell viability and proliferation, acting as a protective mechanism of trophoblasts against the cytotoxicity of etoposide (VP-16). Such a phenomenon was effectively abrogated by autophagy inhibitors 3-methyladenine (3-MA) and chloroquine (CQ). In conclusion, PLAC8-induced autophagy to promote the proliferation of trophoblasts. This study provided insights into the mechanism of PLAC8-induced autophagy in trophoblasts, which is significant for a wide range of gestational diseases and may contribute to developing novel treatment strategies for trophoblastic diseases.
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http://dx.doi.org/10.1096/fj.202002075RRDOI Listing
March 2021

MiR-204-5p Performs a Protective Effect on Cerulein-Induced Rat Pancreatic Acinar Cell AR42J Cell Damage by Targeting Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Gamma and Regulating PI3K/Hippo Pathways.

Pancreas 2021 Feb;50(2):243-250

Department of Gastroenterology, The Second People's Hospital of Jingmen, Jingmen, China.

Objective: This research plans to address the function of miR-204-5p/tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma (YWHAG) in cerulein-induced acute pancreatitis (AP).

Methods: Rat pancreatic acinar cell AR42J was stimulated by 100 nmol/L of cerulein to mimic the situation in AP. Gene Expression Omnibus database was used to select differentially expressed genes. StarBase database and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were used to select the target genes of miR-204-5p, which were further affirmed by dual luciferase assay. The biological behaviors of AR42J cells were measured by cell proliferation and flow cytometry assays. Quantitative real-time polymerase chain reaction and western blot assays were executed to assess YWHAG expression. The secretion of C-C Motif Chemokine Ligand 2/Timp metallopeptidase inhibitor 1 in AR42J cells was evaluated by enzyme-linked immunosorbent assay. The protein expression of YAP1/p-YAP1/PI3K/p-PI3K was measured by western blot.

Results: miR-204-5p expression was profoundly reduced in cerulein-induced AP model. YWHAG was upregulated in cerulein-induced AP model and related to C-C Motif Chemokine Ligand 2/Timp1. In addition to the negative association between miR-204-5p and YWHAG, the alleviation impact of miR-204-5p mimic on cerulein-induced AR42J cell damage was blocked by YWHAG overexpression and PI3K/Hippo signaling pathways activation.

Conclusions: These observations indicated that the alleviation impact of miR-204-5p on cerulein-induced AR42J cell damage was mediated via YWHAG and PI3K/Hippo signaling pathways.
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http://dx.doi.org/10.1097/MPA.0000000000001748DOI Listing
February 2021

Peak trabecular bone microstructure predicts rate of estrogen-deficiency-induced bone loss in rats.

Bone 2021 Apr 22;145:115862. Epub 2021 Jan 22.

McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States. Electronic address:

Postmenopausal osteoporosis affects a large number of women worldwide. Reduced estrogen levels during menopause lead to accelerated bone remodeling, resulting in low bone mass and increased fracture risk. Both peak bone mass and the rate of bone loss are important predictors of postmenopausal osteoporosis risk. However, whether peak bone mass and/or bone microstructure directly influence the rate of bone loss following menopause remains unclear. Our study aimed to establish the relationship between peak bone mass/microstructure and the rate of bone loss in response to estrogen deficiency following ovariectomy (OVX) surgery in rats of homogeneous background by tracking the skeletal changes using in vivo micro-computed tomography (μCT) and three-dimensional (3D) image registrations. Linear regression analyses demonstrated that the peak bone microstructure, but not peak bone mass, was highly predictive of the rate of OVX-induced bone loss. In particular, the baseline trabecular thickness was found to have the highest correlation with the degree of OVX-induced bone loss and trabecular stiffness reduction. Given the same bone mass, the rats with thicker baseline trabeculae had a lower rate of trabecular microstructure and stiffness deterioration after OVX. Moreover, further evaluation to track the changes within each individual trabecula via our novel individual trabecular dynamics (ITD) analysis suggested that a trabecular network with thicker trabeculae is less likely to disconnect or perforate in response to estrogen deficiency, resulting a lower degree of bone loss. Taken together, these findings indicate that the rate of estrogen-deficiency-induced bone loss could be predicted by peak bone microstructure, most notably the trabecular thickness. Given the same bone mass, a trabecular bone phenotype with thin trabeculae may be a risk factor toward accelerated postmenopausal bone loss.
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http://dx.doi.org/10.1016/j.bone.2021.115862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920939PMC
April 2021

Application of convolution neural network in medical image processing.

Authors:
Jie Liu Hongbo Zhao

Technol Health Care 2021 ;29(2):407-417

School of Medical Technology, Xi'an Medical University, Xi'an, Shaanxi, China.

Background: Convolution neural network is often superior to other similar algorithms in image classification. Convolution layer and sub-sampling layer have the function of extracting sample features, and the feature of sharing weights greatly reduces the training parameters of the network.

Objective: This paper describes the improved convolution neural network structure, including convolution layer, sub-sampling layer and full connection layer. This paper also introduces five kinds of diseases and normal eye images reflected by the blood filament of the eyeball "yan.mat" data set, convenient to use MATLAB software for calculation.

Methodsl: In this paper, we improve the structure of the classical LeNet-5 convolutional neural network, and design a network structure with different convolution kernels, different sub-sampling methods and different classifiers, and use this structure to solve the problem of ocular bloodstream disease recognition.

Results: The experimental results show that the improved convolutional neural network structure is ideal for the recognition of eye blood silk data set, which shows that the convolution neural network has the characteristics of strong classification and strong robustness. The improved structure can classify the diseases reflected by eyeball bloodstain well.
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http://dx.doi.org/10.3233/THC-202657DOI Listing
January 2021

Overexpression of LVRN impedes the invasion of trophoblasts by inhibiting epithelial-mesenchymal transition.

Acta Biochim Biophys Sin (Shanghai) 2021 Feb;53(2):249-257

Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.

Laeverin (LVRN) was first detected on the outer layer of the chorion laeve and migrating extravillous trophoblasts (EVTs). It is an enzyme that plays an important role in the placentation and pathophysiology of preeclampsia (PE). Previous studies have indicated that LVRN may be required for the invasion of human trophoblast cells. Paradoxically, LVRN was found to be highly expressed in the trophoblasts of PE patients with impaired invasive capacities. In this study, we detected the expression of LVRN in the placentas of PE patients (n=5) and normal term pregnancy women (n=5) as a control group by immunohistochemistry. LVRN was elevated in decidua (P=0.0083) and villi (P=0.0079) of PE patients. Next, LVRN was overexpressed via adeno-associated virus-mediated gene transfer in trophoblastic cell lines HTR8, Swan71, and JAR. Matrigel transwell assay and wound healing assay showed that overexpression of LVRN impeded the invasion of these three cell lines. Western blot analysis showed that LVRN overexpression caused downregulation of N-cadherin and vimentin and upregulation of E-cadherin, suggesting the inhibitory role of LVRN in epithelial-mesenchymal transition (EMT). Moreover, our data indicated that long noncoding RNA NONSTAT103348 (lnc10-7) was elevated in PE patients. Silencing lnc10-7 led to decreased LVRN expression. Taken together, although the basal level of LVRN may be crucial for cell invasion, overexpression of LVRN may abrogate the cell invasiveness, suggesting a multifaceted role of LVRN in the pathogenesis of PE.
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http://dx.doi.org/10.1093/abbs/gmaa167DOI Listing
February 2021

Mineralogical phase transformation of Fe containing sphalerite at acidic environments in the presence of Cu.

J Hazard Mater 2021 02 22;403:124058. Epub 2020 Sep 22.

School of Minerals Processing & Bioengineering, Central South University, Changsha, Hunan, China; Key Lab of Biohydrometallurgy of Ministry of Education, Changsha, Hunan, China.

Dissolution of the exposed sphalerite (marmatite) in abandoned mining sites and tailings may exacerbate acid and metalliferous drainage (AMD) hazards. Cupric ions are inevitable ions in AMD systems but its action mechanism on the dissolution of sphalerite is still unclear. In this work, the possible phase transition from sphalerite to chalcopyrite is firstly discovered in acidic cupric ions solution according to the results of Raman and (synchrotron radiation-based) X-ray (micro-) diffractometer spectra, which should be an important reason that mediates the dissolution of sphalerite. Results of DFT calculations reveal the underlying mechanism that Cu can selectively replace zinc in marmatite lattices and further diffuse into the matrix. Additionally, a strong correlation between the cupric ion consumption with the pH value variation is discussed and the effects of the formed new phase on the dissolution kinetics of marmatite were researched. According to this work, the action mechanism of cupric ions on sphalerite dissolution in acidic environments is furtherly clarified.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124058DOI Listing
February 2021

Real-Time Volumetric Thermoacoustic Imaging and Thermometry Using a 1.5-D Ultrasound Array.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Apr 29;68(4):1234-1244. Epub 2021 Mar 29.

Noninvasive thermal therapies for the treatment of breast cancer depend on accurate monitoring of tissue temperature to optimize treatment and ensure safety. This work describes a real-time system for 3-D thermoacoustic imaging and thermometry (TAI-TAT) for tracking temperature in tissue samples during heating. The study combines a 2.7-GHz microwave pulse generator with a custom 1.5-D 0.6 MHz ultrasound array for generating and detecting TA signals. The system is tested and validated on slabs of biological tissue and saline gel during heating. Calibration curves for relating the TA signal to temperature were calculated in saline gel (3.40%/°C), muscle (1.73%/°C), and fat (1.15%/°C), respectively. The calibrations were used to produce real-time, volumetric temperature maps at ~3-s intervals with a spatial resolution of approximately 3 mm. TAT temperature changes within a region of interest were compared to adjacent thermocouples with a mean error of 17.3%, 13.2%, and 20.4% for muscle, gel, and fat, respectively. The TAT algorithm was also able to simultaneously track temperatures in different tissues. With further development, noninvasive TAI-TAT may prove to be a valuable method for accurate and real-time feedback during breast cancer ablation therapy.
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http://dx.doi.org/10.1109/TUFFC.2020.3038053DOI Listing
April 2021

Mediastinoscopy-assisted transhiatal esophagectomy versus thoraco-laparoscopic esophagectomy for esophageal cancer: a single-center initial experience.

J Thorac Dis 2020 Sep;12(9):4908-4914

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, China.

Background: We aimed to compare mediastinoscopy-assisted transhiatal esophagectomy (MATHE) with thoraco-laparoscopic esophagectomy (TLE) for patients with esophageal cancer in terms of the clinical effectiveness and perioperative complications.

Methods: In total, 98 patients who underwent esophagectomy consecutively for esophageal squamous cell carcinoma in our center from Jan. 2018 to Dec. 2019 were included in this study. Thirty patients underwent mediastinoscopy-assisted and laparoscopic transhiatal esophagectomy with cervical anastomosis (the MATHE group). The other sixty-eight patients received TLE (the TLE group). Each patient's general conditions and perioperative complications were recorded.

Results: Patients in the MATHE group were observed to have a higher incidence of postoperative hoarseness than those in the TLE group. There were no significant differences between the MATHE group and the TLE group in regards to the operation time, intraoperative blood loss, number of lymph nodes dissected or postoperative hospital stay. Similarly, no statistically significant differences were observed in the incidence of anastomotic fistula, respiratory complications, or chylothorax or in the conversion rate or in-hospital mortality rate between the two groups.

Conclusions: The short-term efficacy in the MATHE group was similar to that in the TLE group, although patients in the MATHE group may have had a higher incidence of postoperative hoarseness. Therefore, MATHE may be a feasible and safe surgical procedure for appropriate patients with esophageal cancer.
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http://dx.doi.org/10.21037/jtd-20-1328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578494PMC
September 2020

HMGR overexpression and interference affect the expression of steroidogenic genes and cholesterol content in bovine intramuscular adipocytes.

Sci Rep 2020 10 6;10(1):16606. Epub 2020 Oct 6.

Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Lichen Region, Jinan, 250100, China.

Previously, we found that mevalonic acid stimulates 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGR) expression in bovine intramuscular adipocytes to influence adipocyte differentiation. However, any direct links among HMGR, steroidogenic genes, and cholesterol content remain unclear. RNA-Seq was conducted to determine the differences between the gene expression profiles of bovine adipocytes containing different HMGR expression constructs. In total, 10,234 differentially expressed genes (DEGs) were found. Of these, 35 and 6 DEGs between the control and the overexpression groups were functionally related to lipid and energy metabolism, respectively. In addition, 43 and 8 DEGs between the control and the HMGR inhibition groups were related to lipid and energy metabolism, respectively. Several DEGs related to lipid and energy metabolism were also identified between the HMGR overexpression group and the HMGR interference group, and many DEGs were correlated positively or negatively with the overexpression or inhibition of HMGR. We also found that, following the activation or inhibition of the HMGR gene, AMP-activated protein kinase (AMPK) and sirtuin type 1 (SIRT1) had opposite expression patterns in bovine intramuscular adipocytes. Interestingly, the HMGR gene was downregulated when HMGR was overexpressed, and upregulated when HMGR was inhibited. Our findings establish a theoretical understanding of signaling pathways involved in cholesterol synthesis by elucidating the relationships between key genes.
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http://dx.doi.org/10.1038/s41598-020-73626-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538946PMC
October 2020

Promotion of the occurrence of endometrioid carcinoma by S100 calcium binding protein P.

BMC Cancer 2020 Sep 3;20(1):845. Epub 2020 Sep 3.

Obstetrics and Gynecology Hospital, Fudan University, Postal address: 413 Zhaozhou Road, Shanghai, 200011, China.

Background: Endometrial cancer, one of the most common malignant tumors, is a serious threat to women's health. Endometrial hyperplasia is a precursor of endometrial cancer. S100 calcium binding protein P (S100P) has been found to play important roles in many types of cancer. The present study aimed to investigate the expression of S100P in endometrial cancer and its precursor lesions, and to explore the possible mechanisms.

Methods: We collected paraffin sections of normal endometrium, simple and complex non-atypical hyperplasia, atypical hyperplasia, and endometrioid carcinoma. The expression of S100P in endometrial cancer and its precancerous lesions was observed using immunohistochemistry. We also cultured primary endometrial cells and endometrial cancer cell lines (Ishikawa and RL95-2), and observed the expression of S100P in these cells. Laser confocal microscopy was used to observe the co-localization of S100P and its interacting protein Ezrin in RL95-2 cells. We employed lentiviruses to knockdown and overexpress S100P and then detected the F-actin distribution and cell invasion using phalloidin staining and Transwell assays.

Results: There was a gradual increase in the S100P signal as the disease progressed from normal endometrium and simple non-atypical hyperplasia, to complex non-atypical hyperplasia, atypical hyperplasia, and then to endometrial cancer. S100P was mainly distributed in the cytoplasm and co-localized with Ezrin in endometrial cancer cells. After knocking down S100P, F-actin aggregated in the nucleus or to the local cell membrane. Furthermore, knockdown of S100P in Ishikawa cells decreased their cell invasion capability. Meanwhile, S100P overexpression in endometrial stromal cells increased cell invasion.

Conclusions: These data suggested that S100P might be involved in the occurrence and development of endometrial cancer via interaction with Ezrin and re-organization of F-actin to promote cell invasion.
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http://dx.doi.org/10.1186/s12885-020-07350-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650527PMC
September 2020

Integrated Bioinformatics Analysis Reveals Function and Regulatory Network of miR-200b-3p in Endometriosis.

Biomed Res Int 2020 29;2020:3962953. Epub 2020 Jul 29.

Biomedical Engineering Research Center, Kunming Medical University, Kunming 650500, China.

Objective: MicroRNAs play vital roles in the development of endometriosis. It is reported that miR-200b-3p is downregulated in endometriosis, although its mechanisms in this disease remain still unclear. Therefore, the purpose of this study was to explore the function and potential regulatory network of miR-200b-3p in endometriosis through database analysis.

Methods: The endometriosis gene expression profiles were downloaded from the GEO database to screen differentially expressed genes (DEGs). The predicted and validated target genes of miR-200b-3p were obtained from miRWalk and miRTarBase database. Then, a comparison was performed between miR-200b-3p target genes and DEGs. GO enrichment and KEGG pathway analysis of the target genes was performed using clusterProfiler package. STRING was used to predict the protein-protein interaction among the proteins encoded by the target genes. Then, TransmiR, LncBase, StarBase, PROMO, and AnimalTFDB were employed to identify interactive transcription factors and lncRNAs of miR-200b-3p.

Results: miR-200b-3p was associated with the transcription factors DNMT1, EZH2, HNF1B, JUN, MYB, ZEB1, and ZEB2 during the pathogenesis of endometriosis. The downstream 110 target genes were involved in the biological processes of positive regulation of MAPK cascade, muscle cell proliferation, organ growth, vasculogenesis, and axon development. KEGG analysis revealed that the main pathways related to miR-200b-3p were microRNAs in cancer, PI3K-Akt signaling pathway, colorectal cancer, and tight junction. In addition, four lncRNAs such as MALAT1, NEAT1, SNHG22, and XIST interacted with miR-200b-3p and were associated with transcription factors FOXP3 and YY1.

Conclusion: The predicted target genes and molecular regulatory network of miR-200b-3p in endometriosis not only revealed its biological function but also provided a valuable guideline for further research.
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http://dx.doi.org/10.1155/2020/3962953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414375PMC
April 2021

The Role of Caspase-4 and NLRP1 in MCF7 Cell Pyroptosis Induced by hUCMSC-Secreted Factors.

Stem Cells Int 2020 9;2020:8867115. Epub 2020 Jul 9.

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan & School of Medicine, Yunnan University, Kunming, 650091 Yunnan, China.

Mesenchymal stem cells (MSCs) are being widely investigated for the development of novel therapeutic approaches for different cancers, including breast cancer, the leading form of cancer in women. Our previous study showed that the factors secreted by human umbilical cord MSCs (hUCMSCs) induced pyroptosis in the breast cancer cell line MCF7 and our RNA sequencing studies revealed an increase in the expression of the pyroptosis-related gene caspase-4 () and nucleotide-binding, leucine-rich repeat pyrin domain-containing protein 1 () in pyroptotic MCF7 cells. Cellular pyroptosis can occur via the canonical pathway (involving caspase-1 and NLRP1) or the noncanonical pathway (involving caspase-4). In this study, we first confirmed that the inflammasome complex formed by NLRP1 and ASC is involved in MCF7 cell pyroptosis induced by hUCMSC-CM. Further, we investigated the role of and in MCF7 cell pyroptosis induced by hUCMSC-secreted factors using shRNA-mediated transfection of or in MCF7 cells. Cytotoxicity analyses revealed that neither knockdown nor NLRP1 knockdown could inhibit the hUCMSC-CM-induced pyroptosis in MCF7 cells. Gene and protein expression analysis showed that hUCMSC-CM induced pyroptosis mainly via the canonical pathway in knockdown MCF7 cells but mainly via the noncanonical pathway in knockdown MCF7 cells. Our study provides a foundation for further studies aimed at elucidating the precise mechanism underlying hUCMSC-induced pyroptosis in breast cancer cells and aid the identification of potential therapeutic targets for breast cancer.
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http://dx.doi.org/10.1155/2020/8867115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368222PMC
July 2020

Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans.

BMC Plant Biol 2020 Jul 16;20(1):337. Epub 2020 Jul 16.

Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, Zhejiang, P.R. China.

Background: Sweet osmanthus (Osmanthus fragrans Lour.) is one of the top ten traditional ornamental flowers in China. The flowering time of once-flowering cultivars in O. fragrans is greatly affected by the relatively low temperature, but there are few reports on its molecular mechanism to date. A hypothesis had been raised that genes related with flower opening might be up-regulated in response to relatively low temperature in O. fragrans. Thus, our work was aimed to explore the underlying molecular mechanism of flower opening regulated by relatively low temperature in O. fragrans.

Results: The cell size of adaxial and abaxial petal epidermal cells and ultrastructural morphology of petal cells at different developmental stages were observed. The cell size of adaxial and abaxial petal epidermal cells increased gradually with the process of flower opening. Then the transcriptomic sequencing was employed to analyze the differentially expressed genes (DEGs) under different number of days' treatments with relatively low temperatures (19 °C) or 23 °C. Analysis of DEGs in Gene Ontology analysis showed that "metabolic process", "cellular process", "binding", "catalytic activity", "cell", "cell part", "membrane", "membrane part", "single-organism process", and "organelle" were highly enriched. In KEGG analysis, "metabolic pathways", "biosynthesis of secondary metabolites", "plant-pathogen interaction", "starch and sucrose metabolism", and "plant hormone signal transduction" were the top five pathways containing the greatest number of DEGs. The DEGs involved in cell wall metabolism, phytohormone signal transduction pathways, and eight kinds of transcription factors were analyzed in depth.

Conclusions: Several unigenes involved in cell wall metabolism, phytohormone signal transduction pathway, and transcription factors with highly variable expression levels between different temperature treatments may be involved in petal cell expansion during flower opening process in response to the relatively low temperature. These results could improve our understanding of the molecular mechanism of relatively-low-temperature-regulated flower opening of O. fragrans, provide practical information for the prediction and regulation of flowering time in O. fragrans, and ultimately pave the way for genetic modification in O. fragrans.
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http://dx.doi.org/10.1186/s12870-020-02549-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367400PMC
July 2020

Fabrication of an injectable BMSC-laden double network hydrogel based on silk fibroin/PEG for cartilage repair.

J Mater Chem B 2020 07;8(27):5845-5848

School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, 230026, China.

An injectable BMSC-encapsulated double network (DN) hydrogel was fabricated via silk fibroin (SF) and poly(ethylene glycol) (PEG), which could efficiently support the survival and proliferation of BMSCs in vitro as well as cartilage repair in vivo, and provides a new strategy for cartilage tissue engineering.
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http://dx.doi.org/10.1039/d0tb01017kDOI Listing
July 2020

A randomized double-blind placebo-controlled multicenter trial of Bushen Yisui and Ziyin Jiangzhuo formula for constipation in Parkinson disease.

Medicine (Baltimore) 2020 Jul;99(28):e21145

Parkinson Medical Center, Beijing Rehabilitation Hospital, Capital Medical University.

Introduction: Constipation is a common nonmotor symptom of Parkinson disease (PD). Constipation can also impact patient's quality of life. Chinese herbal medicines have been used for the treatment of constipation in PD. This trial will evaluate the efficacy and safety of a Chinese herbal formula Bushen Yisui and Ziyin Jiangzhuo (BYZJ) for the treatment of constipation in PD.

Methods And Analysis: This randomized, double-blind, placebo-controlled, multicenter clinical trial will involve 4 hospitals in Beijing, China. The study will aim to recruit 90 PD patients with constipation between 30 and 80 years-of age with a score of 1 - 4 on the Hoehn and Yahr scale. Once recruited, Patients will be randomized into a BYZJ group or a placebo group in a 2:1 ratio. The trial will include a 1-week run-in period, a 4-week double-blind treatment period, a 4-week and a 12-week follow-up period. All patients will be educated about PD-related constipation during the run-in period. BYZJ granules and simulated granules will be administered twice daily for 4 weeks to the BYZJ group and the placebo group respectively. Assessments will be performed during run-in period, before the start of treatment (baseline, week 0), and at 4, 8, and 16 weeks. The primary outcome will be measured with the Constipation Severity Instrument, and secondary outcomes will be evaluated with the Patient Assessment of Constipation Quality of Life questionnaire, Bristol Stool Form Scale, Movement Disorders-Unified Parkinson Disease Rating Scale, Nonmotor Symptoms Scale, PD Sleep Scale, Parkinson Fatigue Scale-16. Laxative use (dose and frequency) will also be recorded. Intention-to-treat and per-protocol set analyses will be used to compare symptom improvement between the 2 groups. Any adverse events will be recorded.

Discussion: If found effective and safe, BYZJ formula will be one of Chinese herb to treat constipation and even other nonmotor or motor symptoms in PD patients. The results will sustain the broader use of BYZJ formula in PD.
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http://dx.doi.org/10.1097/MD.0000000000021145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360320PMC
July 2020

Nanocomposite hydrogels for tissue engineering applications.

Nanoscale 2020 Jul;12(28):14976-14995

CAS Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.

Tissue engineering is an important field of regenerative medicine, which combines scaffolds and cell transplantation to develop substitute tissues and/or promote tissue regeneration. Hydrogels, a three-dimensional network with high water content and biocompatibility, have been widely used as scaffolds to mimic the structure and properties of tissues. However, the low mechanical strength and limited functions of traditional hydrogels greatly limited their applications in tissue engineering. Recently, nanocomposite hydrogels, with its advantages of high mechanical property and some unique properties (such as electrical conductivity, antibacterial, antioxidation, magnetic responsiveness), have emerged as the most versatile and innovative technology, which provides a new opportunity as a unique tool for fabricating hydrogels with excellent properties. In this review, we summarize the recent advances in fabricating nanocomposite hydrogels and their applications in tissue engineering. In addition, the future and prospects of nanocomposite hydrogels for tissue engineering are also discussed.
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http://dx.doi.org/10.1039/d0nr03785kDOI Listing
July 2020

Apoplastic maize fructan exohydrolase Zm-6-FEH displays substrate specificity for levan and is induced by exposure to levan-producing bacteria.

Int J Biol Macromol 2020 Nov 3;163:630-639. Epub 2020 Jul 3.

College of Horticulture, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Fructan exohydrolases (FEHs) are structurally related to cell wall invertases. While the latter are ubiquitous in higher plants, the role of FEHs in non-fructan species has remained enigmatic. To explore possible roles of FEHs in maize, a full length putative Zm-6-FEH-encoding cDNA was cloned displaying high sequence similarity with cell wall invertases. For functional characterization, Zm-6-FEH protein was expressed in Picha pastoris and in Nicotiana benthamiana leaves. Enzyme activity of recombinant Zm-6-FEH protein showed a strong preference for levan as substrate. Expression profiling in maize seedlings revealed higher transcript amounts in the more mature leaf parts as compared to the growth zone at the base of the leaf, in good correlation with FEH enzyme activities. Subcellular localization analysis indicated Zm-6-FEH location in the apoplast. Noteworthy, incubation of leaf discs with levan and co-incubation with high levan-producing bacteria selectively up-regulated transcript levels of Zm-6-FEH, accompanied by an increase of 6-FEH enzyme activity. In summary, the results indicate that Zm-6-FEH, a novel fructan exohydrolase of a non-fructan species, may have a role in plant defense against levan-producing bacteria.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.254DOI Listing
November 2020

In vitro degradability of corn silage and Leymus chinensis silage and evaluation of their mixed ratios on performance, digestion and serum parameters in beef cattle.

J Anim Physiol Anim Nutr (Berl) 2020 Nov 11;104(6):1628-1636. Epub 2020 Jun 11.

Animal Husbandry and Veterinary Institute, Heilongjiang Academy of Land Reclamation Sciences, Harbin, China.

This study investigated the degradability of corn silage (CS) and Leymus chinensis silage (LS) in vitro, and evaluated the effect of various ratios on growth performance, digestion and serum parameters in beef cattle. A 72-hr bath culture trial was performed to evaluate degradability and rumen fermentation characteristics of CS, LS and their combinations [67:33, 33:67, dry matter (DM) basis]. Forty Simmental steers, averaging 441.46 ± 4.45 kg of body weight (BW), were randomly allocated into four dietary treatments for 120-d period. Diets were given as total mixed rations with a forage-to-concentrate ratio of 60:40 and CS:LS ratios of 100:0, 67:33, 33:67 and 0:100 (DM basis). The in vitro trial showed that DM and neutral detergent fibre (NDF) degradability decreased linearly as LS proportion increased, whereas CP degradability increased linearly. Additionally, increased acid detergent fibre (ADF) degradability was detected at 48 hr of incubation. Increasing the proportion of LS increased rumen liquor pH and decreased volatile fatty acid linearly including acetate, propionate and butyrate, whereas the ammonia-N increased linearly at 12 and 72 hr of incubation. With increasing LS ratio, final BW, average daily gain and feed conversion ratio of steers decreased linearly, whereas DMI was not affected. Additionally, apparent digestibility of DM, organic matter, NDF and ADF linearly and quadratically decreased while ether extract apparent digestibility decreased linearly, and CP apparent digestibility was not affected. Serum glucose and urea nitrogen linearly and quadratically decreased while glutamic-pyruvic transaminase activity linearly decreased as the proportion of LS increased. Other serum parameters including total triglycerides, total cholesterol, total protein, albumin and glutamic-oxalacetic transaminease were not affected. Overall, enhancing ratio of LS caused inferior DM and NDF degradability but improved CP degradability in the combinations of LS and CS. A CS:LS ratio of 67:33 resulted in the best growth performance and nutrient utilization in steers.
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http://dx.doi.org/10.1111/jpn.13392DOI Listing
November 2020

Spruce Galactoglucomannan-Stabilized Emulsions Enhance Bioaccessibility of Bioactive Compounds.

Foods 2020 May 23;9(5). Epub 2020 May 23.

Department of Food and Nutrition, University of Helsinki, P.O. Box 66, 00790 Helsinki, Finland.

The increasing public awareness of health and sustainability has prompted the development of functional foods rich in health-promoting ingredients. Processing technologies and sustainable multifunctional ingredients are needed for structuring these formulations. Spruce galactoglucomannan (GGM), the main hemicelluloses in softwood cell walls, are an abundantly available, emerging sustainable food hydrocolloid that have the ability to efficiently emulsify and stabilize oil-in-water emulsions. In this study, we illustrate how this lignocellulosic stabilizer affects the digestion of polyunsaturated fatty acids (PUFAs) in vitro. A 100% decrease in the initial TAG content was observed during the in vitro digestion, suggesting that complete hydrolysis of the TAGs was achieved by the digestive enzymes. Besides, no release of mono-, di-, and oligosaccharides or phenolic compounds from GGM was detected. Our results demonstrate that the GGM-stabilized emulsion could potentially deliver lipophilic bioactive ingredients and enhance their bioaccessibility. In addition, this bio-stabilizer itself would remain stable in the upper gastrointestinal track and serve as a prebiotic for gut microbiota. We anticipate GGM to complement or even replace many of the conventional carriers of bioactive components in future health care products and functional foods.
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http://dx.doi.org/10.3390/foods9050672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278669PMC
May 2020

Structural Phase Transition of Multilayer VSe.

ACS Appl Mater Interfaces 2020 Jun 7;12(22):25143-25149. Epub 2020 May 7.

Physics Department, University of Hong Kong, Hong Kong SAR, P. R. China.

Vanadium diselenide (VSe), a member of the transition metal dichalcogenides (TMDs) family, is emerging as a promising two-dimensional (2D) candidate for the electronic and spintronic device with exotic properties including charge/spin density wave and ferromagnetism. The bulk crystal VSe exists in a crystallographic form of -phase with metallic behavior. In this paper, we report a structural phase transition of multilayer VSe from to through annealing at 650 K, accompanying a metal-insulator transition. We observe that the -phase is more thermodynamically favorable than the -phase at 2D.
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http://dx.doi.org/10.1021/acsami.0c04449DOI Listing
June 2020

An injectable BMSC-laden enzyme-catalyzed crosslinking collagen-hyaluronic acid hydrogel for cartilage repair and regeneration.

J Mater Chem B 2020 05 9;8(19):4237-4244. Epub 2020 Apr 9.

School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, 230026, China.

Articular cartilage has limited self-healing ability due to its lack of abundant nutrients and progenitor cells. In this study, an injectable hydrogel system consisting of collagen type I-tyramine (Col-TA) and hyaluronic acid-tyramine (HA-TA) was fabricated as the bone marrow mesenchymal stem cell (BMSC)-laden hydrogel system for cartilage regeneration. Next, the physiochemical properties of this hydrogel system were well characterized and optimized, including gelation time, stiffness, water absorption and degradability. Further, the proliferation and differentiation of BMSCs within the Col-HA hydrogel were evaluated, and the ability of in vivo cartilage repair was also examined in the presence of the transforming growth factor-β1 (TGF-β1). These results illustrate that this hydrogel can offer a great microenvironment for BMSC growth and cartilage differentiation both in vitro and in vivo, and the Col-HA hydrogel can serve as an ideal hydrogel for cartilage tissue regeneration.
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http://dx.doi.org/10.1039/d0tb00291gDOI Listing
May 2020

Exploring cisplatin resistance in ovarian cancer through integrated bioinformatics approach and overcoming chemoresistance with sanguinarine.

Am J Transl Res 2020 15;12(3):923-939. Epub 2020 Mar 15.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Kunming Medical University Kunming, Yunnan Province, P. R. China.

Ovarian cancer is refractory in response towards platinum-based chemotherapy, and resistance frequently develops. We attempted to identify the driving pathways in cisplatin-resistant ovarian cancer and develop targeted therapies to overcome this resistance. Using an integrated bioinformatics approach, a GSE15372 database from NCBI's Gene Expression Omnibus database was obtained for identifying differentially expressed genes (DEGs), in which 535 DEGs were found (407 up-regulated and 128 down-regulated) in association with ovarian cancer cisplatin-resistance. Gene ontology and pathway enrichment analyses further found that aberrant activation of EGFR/ErbB2 signaling was the driving event in resistant cells. A network of dysregulated genes was built based on these identified DEGs and protein-protein interaction network, which led to the identification of 7 potential inhibitors based on screening a 77 small molecule natural product library. Sanguinarine, alone and in combination with cisplatin, was found to significantly suppress the proliferation of wt/resistant ovarian cancer cells and the growth of parental and resistant ovarian xenograft tumors . Our study suggests that EGFR/ErbB2 activation is one of the driving pathways in developing cisplatin-resistance in ovarian cancer, and that sanguinarine has the potential to be developed as an effective therapy to overcome this therapeutic resistance.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137043PMC
March 2020

Effects of Exogenous Abscisic Acid (ABA) on Carotenoids and Petal Color in 'Yanhonggui'.

Plants (Basel) 2020 Apr 4;9(4). Epub 2020 Apr 4.

Department of Ornamental Horticulture, School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Lin'an 311300, China.

is a well-known native plant in China, and carotenoids are the main group of pigments in the petals. Abscisic acid (ABA) is one of the products of the metabolic pathway of carotenoids. Application of ABA could affect pigmentation of flower petals by changing the carotenoid content. However, little is known about the effects of ABA treatment on carotenoid accumulation in . In this study, different concentrations of ABA (0, 150 and 200 mg/L) were spread on the petals of 'Yanhonggui'. The petal color of 'Yanhonggui' receiving every ABA treatment was deeper than that of the control. The content of total carotenoids in the petals significantly increased with 200 mg/L ABA treatment. In the petals, α-carotene and β-carotene were the predominant carotenoids. The expression of several genes involved in the metabolism of carotenoids increased with 200 mg/L ABA treatment, including , , , , , and . However, the transcription levels of the latter two carotenoid degradation-related genes were much lower than of the five former carotenoid biosynthesis-related genes; the finding would explain the significant increase in total carotenoids in 'Yanhonggui' petals receiving the 200 mg/L ABA treatment.
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http://dx.doi.org/10.3390/plants9040454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238031PMC
April 2020

Large-scale range diffraction grating displacement sensor based on polarization phase-shifting.

Appl Opt 2020 Jan;59(2):469-473

A method is proposed and demonstrated to improve a diffraction grating displacement sensor to simultaneously achieve nanometer-level resolution and an extended range of operation. The method exploits the polarization phase-shifting optical path to extract two sinusoidal signals with a quadrature phase shift. The interpolation circuit is applied to nonlinearly convert two sinusoidal signals into a standard incremental AB quadrature digital signal, implementing an extended operation range with the magnitude of a laser coherence length. This work enables displacement measurement operated at large-scale range, and provides a significant guide for the design of a high performance micro-displacement sensor.
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http://dx.doi.org/10.1364/AO.59.000469DOI Listing
January 2020