Publications by authors named "Hongbo Liu"

389 Publications

Exploring the genic resources underlying metabolites through mGWAS and mQTL in wheat: From large-scale gene identification and pathway elucidation to crop improvement.

Plant Commun 2021 Jul 30;2(4):100216. Epub 2021 Jun 30.

National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.

Common wheat ( L.) is a leading cereal crop, but has lagged behind with respect to the interpretation of the molecular mechanisms of phenotypes compared with other major cereal crops such as rice and maize. The recently available genome sequence of wheat affords the pre-requisite information for efficiently exploiting the potential molecular resources for decoding the genetic architecture of complex traits and identifying valuable breeding targets. Meanwhile, the successful application of metabolomics as an emergent large-scale profiling methodology in several species has demonstrated this approach to be accessible for reaching the above goals. One such productive avenue is combining metabolomics approaches with genetic designs. However, this trial is not as widespread as that for sequencing technologies, especially when the acquisition, understanding, and application of metabolic approaches in wheat populations remain more difficult and even arguably underutilized. In this review, we briefly introduce the techniques used in the acquisition of metabolomics data and their utility in large-scale identification of functional candidate genes. Considerable progress has been made in delivering improved varieties, suggesting that the inclusion of information concerning these metabolites and genes and metabolic pathways enables a more explicit understanding of phenotypic traits and, as such, this procedure could serve as an -omics-informed roadmap for executing similar improvement strategies in wheat and other species.
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http://dx.doi.org/10.1016/j.xplc.2021.100216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299079PMC
July 2021

Effects of Dexmedetomidine on Patients Undergoing Laparoscopic Surgery for Colorectal Cancer.

J Surg Res 2021 Jul 15;267:687-694. Epub 2021 Jul 15.

Department of Anesthesiology, The First People's Hospital of Changde City, Changde, China. Electronic address:

Background: Pneumoperitoneum during laparoscopic surgery has a systemic impact on the renal system and might contribute to acute kidney injury or postoperative renal dysfunction. However, effective preventive strategies are still lacking. We aimed to explore the effects of dexmedetomidine (DEX) on kidney and other organ function in patients undergoing elective laparoscopic surgery for colorectal cancer.

Materials And Methods: Fifty-six patients were randomly enrolled into the Control or DEX group. The DEX group received 1 µg kg DEX intravenously within 10 min followed by a maintenance dose of 0.5 µg kg h infused until 30 min before closing the peritoneum. In the Control group, 0.9% sodium chloride was administered as a placebo. The primary outcome was serum neutrophil gelatinase-associated lipocalin (NGAL) levels reflecting kidney injury. Secondary outcomes included variables reflecting the kidney, intestinal injury and systemic inflammatory response.

Results: NGAL levels were significantly lower in the DEX group than in the Control group at 1 d and 5 d postoperatively (107.5 ± 55.6 ng mLversus 179.5 ± 78.2 ng mL; 70.3 ± 45.8 ng mLversus 135.2 ± 59.6 ng mL, P < 0.001), while the BUN and Cr levels showed no differences between the groups. Serum DAO activity was significantly lower in the DEX group patients 24 h after surgery. Moreover, I-FABP levels were markedly lower at 2 h and 24 h postoperatively in the DEX group than in the Control group (P < 0.001).

Conclusions: Perioperative DEX administration may potentially confer kidney and intestinal protection during laparoscopic surgery for colorectal cancer patients.
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http://dx.doi.org/10.1016/j.jss.2021.06.043DOI Listing
July 2021

Field tests of crop growth using hydrothermal and spray-dried cephalosporin mycelia dregs as amendments: Utilization of nutrient and soil antibiotic resistome.

Environ Res 2021 Jul 15;202:111638. Epub 2021 Jul 15.

School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, Shanxi, 710049, China. Electronic address:

The disposal and reuse of cephalosporin mycelia dregs (CMDs) pose a great challenge to the biopharma industry, but it acts as the new source of antibiotic resistome, although agriculture intensification remains uncertain. Herein, two common cash crops (maize and soybean) were planted in the actual field, and the effects of the application of treated CMDs, chicken manure and chemical fertilizer served as control groups were both investigated according to comparison experiment. Amplicon-targeted 16S rRNA and high-throughput sequencing was analyzed for rhizosphere antibiotic resistome. Results showed that hydrothermal and spray-dried (HT + SD) CMDs could promote nutrients uptake and stabilize soil fertility indicator, and finally improved the crop yield (maximum, 119.68%). The numbers and relative abundances of total ARGs in soils were not significantly different from that of conventional fertilizer (p > 0.05), but crop type marked the differences in distribution. The overall economic benefits are predicted to be around $373-745 million annually, considering its application to the whole country. HT + SD-treated CMDs can be therefore used as a high-quality and safe alternative fertilizer for agriculture use. These findings are expected to offer a fresh perspective on the application of antibiotic fermentation residue (AFR) in the future.
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http://dx.doi.org/10.1016/j.envres.2021.111638DOI Listing
July 2021

The rice Raf-like MAPKKK OsILA1 confers broad-spectrum resistance to bacterial blight by suppressing the OsMAPKK4-OsMAPK6 cascade.

J Integr Plant Biol 2021 Jul 16. Epub 2021 Jul 16.

National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.

MAPKKKs are the first components of MAPK cascades, which play pivotal roles in signaling during plant development and physiological processes. The genome of rice encodes 75 MAPKKKs, of which 43 are Raf-like MAPKKKs. The functions and action modes of most of the Raf-like MAPKKKs, whether they function as bona fide MAPKKKs and which are their downstream MAPKKs are largely unknown. Here, we identified the osmapkkk43 mutant, which conferred broad-spectrum resistance to Xanthomonas oryzae pv. oryzae (Xoo), the destructive bacterial pathogen of rice. OsMAPKKK43 encoding a Raf-like MAPKKK was previously known as Increased Leaf Angle 1 (OsILA1). Genetic analysis indicated that OsILA1 functioned as a negative regulator and acted upstream of the OsMAPKK4-OsMAPK6 cascade in rice-Xoo interactions. Unlike classical MAPKKKs, OsILA1 mainly phosphorylated the threonine 34 site at the N-terminal domain of OsMAPKK4, which possibly influenced the stability of OsMAPKK4. The N-terminal domain of OsILA1 is required for its homodimer formation and its full phosphorylation capacity. Taken together, our findings reveal that OsILA1 acts as a negative regulator of the OsMAPKK4-OsMAPK6 cascade and is involved in rice-Xoo interactions. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13150DOI Listing
July 2021

HMGB1 Translocation is Associated with Tumor-Associated Myeloid Cells and Involved in the Progression of Fibroblastic Sarcoma.

Pathol Oncol Res 2021 31;27:608582. Epub 2021 Mar 31.

Department of Pathology, The Second Affiliated Hospital of Guilin Medical University, Guilin, China.

The morphological variability and genetic complexity of fibroblastic sarcoma makes its diagnosis and treatment a challenge. High-mobility group box 1 protein (HMGB1), which functions as a DNA chaperone and a prototypical damage-associated molecular pattern, plays a paradoxical role in cancer. However, the expression pattern and role of HMGB1 in fibroblastic sarcomas is ill defined. By immunostaining of 95 tissue microarray cores of fibroblastic sarcomas, HMGB1 was found to be expressed in most tumor tissues. Nuclear HMGB1 translocation to cytoplasm was observed both in tumor cells and vascular endothelial cells. A visible number of tumor-associated myeloid cells including CD68 and CD163 macrophages and CD33 myeloid cells were also detected in most tumor tissues. HMGB1 translocation was not only associated with CD68, CD163, and CD33 density, but also with disease progression. These results imply that HMGB1, an important regulator of the tumor microenvironment, is associated with tumor-associated myeloid cells and involved in the progression of fibroblastic sarcomas; HMGB1 may serve as a promising prognostic biomarker and a potential therapeutic target for fibroblastic sarcoma.
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http://dx.doi.org/10.3389/pore.2021.608582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262203PMC
March 2021

LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis.

Cell Mol Biol Lett 2021 Jul 8;26(1):33. Epub 2021 Jul 8.

Handan Central Hospital, Handan, 056001, Hebei Province, China.

Background: Long noncoding RNA (lncRNA), urothelial carcinoma-associated 1 (UCA1) is aberrantly expressed in multiple cancers and has been verified as an oncogene. However, the underlying mechanism of UCA1 in the development of gastric cancer is not fully understood. In the present study, we aimed to identify how UCA1 promotes gastric cancer development.

Methods: The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data were used to analyze UCA1 and myosin VI (MYO6) expression in gastric cancer. Western blot and quantitative real-time PCR (QPCR) were performed to test the expression level of the UCA1/miR-145/MYO6 axis in gastric cancer cell lines and tissues. The roles of the UCA1/miR-145/MYO6 axis in gastric cancer in vitro and in vivo were investigated by CCK-8 assay, flow cytometry, siRNAs, immunohistochemistry, and a mouse xenograft model. The targeted relationship among UCA1, miR-145, and MYO6 was predicted using LncBase Predicted v.2 and TargetScan online software, and then verified by luciferase activity assay and RNA immunoprecipitation.

Results: UCA1 expression was higher but miR-145 expression was lower in gastric cancer cell lines or tissues, compared to the adjacent normal cell line or normal tissues. Function analysis verified that UCA1 promoted cell proliferation and inhibited cell apoptosis in the gastric cancer cells in vitro and in vivo. Mechanistically, UCA1 could bind directly to miR-145, and MYO6 was found to be a downstream target gene of miR-145. miR-145 mimics or MYO6 siRNAs could partly reverse the effect of UCA1 on gastric cancer cells.

Conclusions: UCA1 accelerated cell proliferation and inhibited cell apoptosis through sponging miR-145 to upregulate MYO6 expression in gastric cancer, indicating that the UCA1/miR-145/MYO6 axis may serve as a potential therapeutic target for gastric cancer.
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http://dx.doi.org/10.1186/s11658-021-00275-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268585PMC
July 2021

MUC5B regulates the airway inflammation induced by atmospheric PM in rats and A549 cells.

Ecotoxicol Environ Saf 2021 Sep 23;221:112448. Epub 2021 Jun 23.

Department of Occupational and Environmental Health, School of Public Health, Jilin University, China. Electronic address:

Atmospheric PM can induce airway inflammation and mucin secretion. MUC5B is required for airway defense. However, the research on the role of MUC5B in airway inflammation induced by atmospheric PM remains limited. This study was designed to explore the role of MUC5B in airway inflammation induced by atmospheric PM. In vivo, Wistar rats were exposed to 0, 1.5, 7.5, 37.5 mg/ kg PM saline suspension via intratracheal instillation. HE staining and AB-PAS staining were used to observe the airway inflammation and goblet cell hyperplasia. In vitro, normal A549 cells and MUC5B-knockdown A549 cells were exposed to 0, 100, 200 and 400 μg/mL PM for 6 h, 12 h, 24 h and 48 h. ELISA was used to measure the levels of TNF-α and IL-1β in serum and bronchoalveolar lavage fluid of rats and in cell culture. Real time-PCR and ELISA were used to quantify the mRNA and protein levels of MUC5B in trachea and lung of rats and in A549 cells. PM could cause the infiltration of inflammatory cells and increase the mucus secretions and goblet cell metaplasia. MUC5B is related to rats' airway inflammation induced by PM. A549 cells exposed to PM in higher concentration and longer time, the protein level of MUC5B was significantly increased, while the levels of IL-1β, TNF-α and MUC5B mRNA were significantly decreased. Compared with normal A549 cells, the levels of IL-1β and TNF-α were significantly higher in Muc5b-knockdown cells. Atmospheric PM can induce airway inflammation and mucin secretion. MUC5B played a critical role in controlling the inflammatory response induced by PM.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112448DOI Listing
September 2021

Cadmium bioaccumulation and distribution in the freshwater bivalve Anodonta woodiana exposed to environmentally relevant Cd levels.

Sci Total Environ 2021 Jun 8;791:148289. Epub 2021 Jun 8.

Key Laboratory of Fishery Eco-Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. Electronic address:

Anodonta woodiana is a globally distributed freshwater bivalve, which is a unique bioindicator in the "Freshwater Mussel Watch" project. Numerous countries have used A. woodiana for biomonitoring the aqueous cadmium (Cd) contamination. However, the bioaccumulation and distribution characteristics of environmentally relevant Cd concentrations in the bivalve were unknown. In this study, A. woodiana was exposed to aqueous Cd concentrations (1.1, 2.6, and 5.5 μg/L) for 30 days. The concentrations of Cd in the whole soft tissues were linearly and positively correlated with the aqueous Cd concentrations and exposure time (P < 0.05). Analysis of the organic bioaccumulation and distribution showed that Cd concentrations and proportions in the gills and mantle were linearly and positively correlated with aqueous Cd concentrations and with the Cd concentrations in the whole soft tissues (P < 0.05). Analyses of the subcellular fractions showed that Cd concentrations and proportions in the metal-rich granule (MRG) were linearly and positively correlated with aqueous Cd concentrations and with the Cd concentrations in the whole soft tissues (P < 0.05). This suggests that the gills and mantle could be the main target organs for Cd bioaccumulation, and that the MRG could be the major site for Cd bioaccumulation and distribution. These results improve our understanding of the bioaccumulation and distribution mechanisms of Cd in bivalves.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148289DOI Listing
June 2021

Underestimated humic acids release and influence on anaerobic digestion during sludge thermal hydrolysis.

Water Res 2021 May 28;201:117310. Epub 2021 May 28.

Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, Jiangsu Province, PR China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou 215011, Jiangsu Province, PR China. Electronic address:

Humic-like acids (HAs) are abundant in sewage sludge but mainly bonded with solids. Thus, their influences are often neglected in conventional digestion processes. Currently thermal hydrolysis pretreatment (THP) has been widely adopted in sludge anaerobic digestion (AD) to enhance hydrolysis of complex matters and further to improve methane production. However, the impacts of enhanced release of HAs and the mechanisms involved are not well understood and need to be further investigated because the substantial amounts of HAs present in AD could severely threaten the sludge AD processes. Results in the present study indicated that the concentration of soluble HAs in sludge was elevated by 90 times due to the THP, from 8 mg/L in raw sludge to 727 mg/L in the pretreated sludge hydrolyzed at 180 °C. Moreover, the structural characteristics of soluble HAs, including aromatic condensation degree, elemental composition and functional group, also showed substantial differences with the increased temperature of the THP. Furthermore, the release of HAs presented significant influences on sludge digestion. Acidification rate was inhibited by over 50% with 0.4 g/L of HAs, whereas methanogenesis was improved by nearly 200% with 0.8 g/L HAs and inhibited about 50% with 2.0 g/L. The activities of proteinase and co-enzyme F were decreased by 20% and increased by 19%, respectively, under HAs stress at 0.6 g/L for 5 days. Moreover, molecular structural changes of soluble HAs also contributed to the influences. Especially, the E4/E6 value representing the degree of HAs aromatic condensation and C/N ratio of soluble HAs were closely correlated with their inhibition degree to sludge hydrolysis. The findings of this study demonstrate that the influences of HAs are evident and also vary to the different steps of anaerobic digestion processes, which shall not be negligible during the sludge digestion that is with THP. Due to the rate-limiting step was methanogenesis in the AD process of pretreated sludge by thermal hydrolysis, HAs concentration was recommended at low level, for example around 1.0 g/L, to accelerate or not limit methanogenesis.
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http://dx.doi.org/10.1016/j.watres.2021.117310DOI Listing
May 2021

Clinical and Prognostic Significance of CD117 in Non-Small Cell Lung Cancer: A Systemic Meta-Analysis.

Pathobiology 2021 9;88(4):267-276. Epub 2021 Jun 9.

Department of Oncology, People's Hospital of Xinjiang Uygur, Urumqi, China.

The aim of this study was to assess the relationship of cluster of differentiation 117 (CD117) expression with the clinicopathological characteristics and the prognosis in patients with non-small cell lung cancer (NSCLC). No meta-analysis concerning the correlation of CD117 expression with clinical and prognostic values of the patients with NSCLC is reported. A systematic literature search was conducted to achieve eligible studies. The combined odds ratios (ORs) or hazard ratios (HRs: multivariate Cox analysis) with their 95% confidence intervals (CIs) were calculated in this analysis. Final 17 eligible studies with 4,893 NSCLC patients using immunohistochemical detection were included in this meta-analysis. CD117 expression was not correlated with gender (male vs. female), clinical stage (stages 3-4 vs. stages 1-2), tumor grade (grade 3 vs. grades 1-2), T-stage (T-stages 3-4 vs. T-stages 0-2), distal metastasis, and disease-free survival (DFS) of NSCLC (all p values >0.05). CD117 expression was associated with lymph node metastasis (positive vs. negative: OR = 0.74, 95% CI = 0.56-0.97, p = 0.03), histological type (adenocarcinoma (AC) versus squamous cell carcinoma (SCC): OR = 1.74, 95% CI = 1.26-2.39, p = 0.001), and a worse overall survival (OS) (HR = 1.89, 95% CI = 1.22-2.92, p = 0.004). The expression of CD117 was significantly higher in AC than in SCC. CD117 may be an independent prognostic indicator for worse OS in NSCLC.
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http://dx.doi.org/10.1159/000514386DOI Listing
June 2021

Efficient recovery of phosphate from simulated urine by Mg/Fe bimetallic oxide modified biochar as a potential resource.

Sci Total Environ 2021 Aug 7;784:147546. Epub 2021 May 7.

Aix-Marseille Univ, CNRS, IRD, INRA, Coll France, CEREGE, 13100 Aix en Provence, France.

The massive use of phosphate fertilizers in agriculture is costly and induces water pollution, calling for more sustainable phosphate sources in the context of the circular economy. Here we prepared a new adsorbent based on waste straw biochar modified with the Mg/Fe bimetallic oxide, namely the Mg/Fe biochar, to recover phosphate from the simulated urine as an possible phosphate fertilizer. About 90% phosphate was recovered from the simulated urine with a wide pH range of 3.0-9.0 and a maximum adsorption capacity of 206.2 mg/g, using 1 g/L of Mg/Fe modified biochar. A pseudo second-order kinetics and Sips model were proposed to fit the experimental data well, suggesting that the adsorption was controlled by physical and chemical processes, which is driven by electrostatic attraction, intra-particle diffusion, ion exchange and surface ligand exchange. Overall, the Mg/Fe biochar is renewable and can recover more than 70% of phosphate in the simulated urine after 5 cycles of reuse, which appears as a safe and efficient adsorbent to recycle phosphate from urine.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147546DOI Listing
August 2021

Bardoxolone and bardoxolone methyl, two Nrf2 activators in clinical trials, inhibit SARS-CoV-2 replication and its 3C-like protease.

Signal Transduct Target Ther 2021 05 29;6(1):212. Epub 2021 May 29.

BNLMS, Peking-Tsinghua Center for Life Sciences at College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

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http://dx.doi.org/10.1038/s41392-021-00628-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164054PMC
May 2021

Comprehensive role of thermal combined ultrasonic pre-treatment in sewage sludge disposal.

Sci Total Environ 2021 Oct 19;789:147862. Epub 2021 May 19.

Aix-Marseille Univ, CNRS, IRD, INRA, Coll France, CEREGE, 13100 Aix en Provence, France.

Thermal/ultrasonic pre-treatment of sludge has been proven to break the hydrolysis barriers of sewage sludge (SS) and improve the performance of anaerobic digestion (AD). In this study, the objective was to investigate whether the combination of two pre-treatment methods can achieve better results on the AD of SS. The results indicated that, compared with the control group and separate pre-treatment groups, the thermal combined ultrasonic pre-treatment presented more obvious solubilization of soluble proteins, polysaccharides, and other organic matters in SS. The combined method promoted the dissolution of protein-like substances more effectively, with biogas production increased by 19% and the volatile solid (VS) removal rate improved to above 50% compared with the control group. The capillary suction time is reduced by about 85%, which greatly improved the dewatering performance of SS. In addition, the combined method has advantages in degrading sulfonamide antibiotics, roxithromycin and tetracycline. Particularly, by analyzing the interaction between the degradation of different antibiotics and the composition of dissolved organic matters (DOMs), it was found that the composition of DOMs could affect the degradability of different antibiotics. Among them, the high content of tyrosine-like and tryptophan-like was conducive to the degradation of sulfamethoxazole, and the high content of fulvic acid-like and humic acid-like was conducive to the degradation of roxithromycin and tetracycline. This work evaluated the comprehensive effect of thermal combined ultrasonic pre-treatment on SS disposal and provided useful information for its engineering.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147862DOI Listing
October 2021

Highly fluorescent nitrogen and boron doped carbon quantum dots for selective and sensitive detection of Fe.

J Mater Chem B 2021 Jun;9(23):4654-4662

College of Material Science and Engineering, Hunan University, Hunan, Changsha 410082, China. and Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology, Hunan University, Hunan, Changsha 410082, China.

Due to the essential role of Fe3+ in physiological and pathological processes, the detection of Fe3+ has drawn increasing attention in the field of disease diagnosis and environmental protection. However, most existing methods require either cumbersome sample pretreatment or sophisticated and expensive test equipment. Recently, carbon quantum dots have found a wide range of applications such as nanoprobes for Fe3+ determination, albeit with limited sensitivity and selectivity. Herein, we report core-shell carbon quantum dots B1N2CQDs via a two-step hydrothermal approach using citric acid, boric acid and ethylenediamine as precursors. The obtained B1N2CQDs exhibit excellent water solubility and remarkable stability as well as a high fluorescence quantum yield of 15.4%. In addition, the fluorescence of B1N2CQDs is quenched exclusively by Fe3+ with minimal interference from other metal ions. A linear relationship with R2 = 0.998 was observed between the fluorescence quenching capacity and the Fe3+ concentration in the range of 2-160 μM, with the limit of detection calculated to be 80 nM. Finally, the as-prepared B1N2CQDs were successfully applied as a highly efficient fluorescent probe for Fe3+ detection in river water samples and intracellular Fe3+ imaging in biological systems.
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http://dx.doi.org/10.1039/d1tb00371bDOI Listing
June 2021

Transcriptome-wide association analysis identifies DACH1 as a kidney disease risk gene that contributes to fibrosis.

J Clin Invest 2021 May;131(10)

Department of Medicine, Renal Electrolyte and Hypertension Division, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Genome-wide association studies (GWAS) for kidney function identified hundreds of risk regions; however, the causal variants, target genes, cell types, and disease mechanisms remain poorly understood. Here, we performed transcriptome-wide association studies (TWAS), summary Mendelian randomization, and MetaXcan to identify genes whose expression mediates the genotype effect on the phenotype. Our analyses identified Dachshund homolog 1 (DACH1), a cell-fate determination factor. GWAS risk variant was associated with lower DACH1 expression in human kidney tubules. Human and mouse kidney single-cell open chromatin data (snATAC-Seq) prioritized estimated glomerular filtration rate (eGFR) GWAS variants located on an intronic regulatory region in distal convoluted tubule cells. CRISPR-Cas9-mediated gene editing confirmed the role of risk variants in regulating DACH1 expression. Mice with tubule-specific Dach1 deletion developed more severe renal fibrosis both in folic acid and diabetic kidney injury models. Mice with tubule-specific Dach1 overexpression were protected from folic acid nephropathy. Single-cell RNA sequencing, chromatin immunoprecipitation, and functional analysis indicated that DACH1 controls the expression of cell cycle and myeloid chemotactic factors, contributing to macrophage infiltration and fibrosis development. In summary, integration of GWAS, TWAS, single-cell epigenome, expression analyses, gene editing, and functional validation in different mouse kidney disease models identified DACH1 as a kidney disease risk gene.
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http://dx.doi.org/10.1172/JCI141801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121513PMC
May 2021

Status of Immunotherapy Acceptance in Chinese Patients With Multiple Sclerosis: Analysis of .

Front Neurol 2021 8;12:651511. Epub 2021 Apr 8.

Department of Neurology, Affiliated Zhongshan Hospital, Xiamen University, Xiamen, China.

The prevalence of multiple sclerosis (MS) in China is low, although it has been increasing recently. Owing to the paucity of data on immunotherapy acceptance in the Chinese population, we conducted this study to analyze factors affecting the acceptance of immunotherapy and selection of disease-modifying therapies (DMTs) based on personal and clinical data of patients with MS. In this study, data were obtained from the , which was the first national survey of patients with MS in China. There were 1,212 patients with MS from 31 provinces who were treated at 49 Chinese hospitals over a 4-month period from May 2018 to August 2018, and the patients were asked to complete online questionnaires to assess their understanding of the disease. In general, highly educated patients with frequent relapses were more willing to receive treatment regardless of DMTs or other immunotherapy, and patients with more understanding of the disease opted to be treated. Younger patient population, patients with severe disease course, and those with more symptoms were likely to choose the treatment. Moreover, a higher proportion of women chose to be treated with DMTs than with other immunotherapies. Education status and patient awareness of the disease impact the treatment acceptance in Chinese patients with MS. Therefore, we call for improving the awareness of MS disease and social security to help patients to improve their quality of life.
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http://dx.doi.org/10.3389/fneur.2021.651511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060470PMC
April 2021

Single cell regulatory landscape of the mouse kidney highlights cellular differentiation programs and disease targets.

Nat Commun 2021 04 15;12(1):2277. Epub 2021 Apr 15.

Renal, Electrolyte, and Hypertension Division, Department of Medicine, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, USA.

Determining the epigenetic program that generates unique cell types in the kidney is critical for understanding cell-type heterogeneity during tissue homeostasis and injury response. Here, we profile open chromatin and gene expression in developing and adult mouse kidneys at single cell resolution. We show critical reliance of gene expression on distal regulatory elements (enhancers). We reveal key cell type-specific transcription factors and major gene-regulatory circuits for kidney cells. Dynamic chromatin and expression changes during nephron progenitor differentiation demonstrates that podocyte commitment occurs early and is associated with sustained Foxl1 expression. Renal tubule cells follow a more complex differentiation, where Hfn4a is associated with proximal and Tfap2b with distal fate. Mapping single nucleotide variants associated with human kidney disease implicates critical cell types, developmental stages, genes, and regulatory mechanisms. The single cell multi-omics atlas reveals key chromatin remodeling events and gene expression dynamics associated with kidney development.
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http://dx.doi.org/10.1038/s41467-021-22266-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050063PMC
April 2021

Atomic Sulfur Filling Oxygen Vacancies Optimizes H Absorption and Boosts the Hydrogen Evolution Reaction in Alkaline Media.

Angew Chem Int Ed Engl 2021 Jun 7;60(25):14117-14123. Epub 2021 May 7.

State Key Laboratory of Applied Organic Chemistry, Frontiers Science Center for Rare Isotopes, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.

The hydrogen evolution reaction (HER) usually has sluggish kinetics in alkaline solution due to the difficulty in forming binding protons. Herein we report an electrocatalyst in which sulfur atoms are doping in the oxygen vacancies (V ) of inverse spinel NiFe O (S-NiFe O ) to create active sites with enhanced electron transfer capability. This electrocatalyst has an ultralow overpotential of 61 mV at the current density of 10 mA cm and long-term stability of 60 h at 1.0 Acm in 1.0 M KOH media. In situ Raman spectroscopy revealed that S sites adsorb hydrogen adatom (H*) and in situ form S-H*, which favor the production of hydrogen and boosts HER in alkaline solution. DFT calculations further verified that S introduction lowered the energy barrier of H O dissociation. Both experimental and theoretical investigations confirmed S atoms are active sites of the S-NiFe O .
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http://dx.doi.org/10.1002/anie.202104055DOI Listing
June 2021

Lipidomic and transcriptomic analysis reveals reallocation of carbon flux from cuticular wax into plastid membrane lipids in a glossy "Newhall" navel orange mutant.

Hortic Res 2020 Apr 1;7(1):41. Epub 2020 Apr 1.

Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, China.

Both cuticle and membrane lipids play essential roles in quality maintenance and disease resistance in fresh fruits. Many reports have indicated the modification of alternative branch pathways in epicuticular wax mutants; however, the specific alterations concerning lipids have not been clarified thus far. Here, we conducted a comprehensive, time-resolved lipidomic, and transcriptomic analysis on the "Newhall" navel orange (WT) and its glossy mutant (MT) "Gannan No. 1". The results revealed severely suppressed wax formation accompanied by significantly elevated production of 36-carbon plastid lipids with increasing fruit maturation in MT. Transcriptomics analysis further identified a series of key functional enzymes and transcription factors putatively involved in the biosynthesis pathways of wax and membrane lipids. Moreover, the high accumulation of jasmonic acid (JA) in MT was possibly due to the need to maintain plastid lipid homeostasis, as the expression levels of two significantly upregulated lipases (CsDAD1 and CsDALL2) were positively correlated with plastid lipids and characterized to hydrolyze plastid lipids to increase the JA content. Our results will provide new insights into the molecular mechanisms underlying the natural variation of plant lipids to lay a foundation for the quality improvement of citrus fruit.
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http://dx.doi.org/10.1038/s41438-020-0262-zDOI Listing
April 2020

Trade-off control of organic matter and disinfection by-products in the drinking water treatment chain: Role of pre-ozonation.

Sci Total Environ 2021 May 23;770:144767. Epub 2021 Jan 23.

Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Prague, Czech Republic.

Ozone is a strong oxidant commonly used in drinking water treatment, but its role in the transformation/formation of organic matters (OMs) and disinfection by-products (DBPs) in the drinking water treatment chain had not been systematically studied. In this work the occurrence and building up of OMs, DBPs of trihalomethanes (THMs) and nitrosamines (NAs) during water purification steps under different pre-ozonation dosages have been studied through lab-scale and pilot-scale studies. Results indicated that 0-0.4 mg/L of pre-ozonation dosage could reduce organic load of following-up process steps but insufficient to control DBPs. Seasonal performances of a pilot plant indicated that the accumulation of DBPs was much less in summer than in winter. Furthermore the formation potential of NAs was higher in winter than summer when 0.4 mg/L pre-ozonation was dosed while the maximum removal efficiency of organic matter was found at a pre-ozonation dosage of 0.8 mg/L in summer. Finally a seasonal trade-off control strategy for both OMs and DBPs was proposed with an elucidated role of pre-ozonation in the drinking water treatment chain. This study provided working principles on optimizing pre-ozonation dosage and a seasonal control strategy for trade-off control of both OMs and DBPs in drinking water treatment plants.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144767DOI Listing
May 2021

Realizing Ultralong-Term Cyclicability of 5 Volt-Cathode-Material Graphite Flakes by Uniformly Comodified TiO/Carbon Layer Inducing Stable Cathode-Electrolyte Interphase.

ACS Appl Mater Interfaces 2021 Mar 23;13(8):10101-10109. Epub 2021 Feb 23.

College of Materials Science and Engineering, Hunan University, Changsha 410082, China.

A common issue the high-voltage cathode materials of secondary batteries suffered from is oxidative electrolyte decomposition inducing rapid capacity fading with discharge/charge cycling. Herein, a highly efficient strategy realizing stable cathode-electrolyte interphase (CEI) and ultralong-term cyclicability of 5 volt-cathode-material graphite flakes (GFs) for dual-ion batteries is demonstrated. The TiO/carbon-comodified GF (TO/GF) cathode material with uniform distribution and tight bonding of the nanosized TiO/carbon layer on the GF surface is synthesized, in which the GF surface is partitioned into nanodomains by the uniformly distributed TiO nanoparticles. Meanwhile, the amorphous carbon layer acts as a gummed tape bonding tightly the TiO nanoparticles on the graphite flake surface. Serial electrochemical impedance spectroscopy and structural/chemical analyses demonstrate that these unique structural characteristics of the TiO/carbon comodification endow the TO/GF cathode material with a stable CEI layer coupled with much reduced electrolyte decomposition. Consequently, extremely high cyclicability of 10,000 stable discharge/charge cycles with an extremely low capacity fading rate of 0.0021% for anion PF storage is realized. This efficient strategy has a potential to be extended to other high-voltage cathode materials and further scaled to the industrial level.
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http://dx.doi.org/10.1021/acsami.0c23070DOI Listing
March 2021

MiRNA Polymorphisms and Hepatocellular Carcinoma Susceptibility: A Systematic Review and Network Meta-Analysis.

Front Oncol 2020 19;10:562019. Epub 2021 Jan 19.

Department of Health Statistics, School of Public Health, China Medical University, Shenyang, China.

Background: Hepatocellular carcinoma (HCC) is an intractable public health threat worldwide, representing the second leading cause of cancer-related mortality, with limited early detection and therapeutic options. Recent findings have revealed that the susceptibility of HCC is closely related to microRNA (miRNA). We performed this systematic review with a network meta-analysis to investigated four single nucleotide polymorphisms (SNPs) that most regularly reported in miRNAs, exploring their involvement in HCC susceptibility and interaction with hepatitis B virus (HBV).

Methods: Databases were reviewed for related studies published up to May 2019 to identify all studies that compared genotypes of miR-146a rs2910164, miR-149 rs2292832, miR-196a2 rs11614913, and miR-499 rs3746444 with no language and date restrictions. A pairwise meta-analysis was performed to estimate pooled odds ratios and 95% confidence intervals incorporating heterogeneity to assess the relationship between four miRNA polymorphisms and HCC. To further clarify the effect of polymorphisms on HCC, a Bayesian network meta-analysis was conducted to combine the effective sizes of direct and indirect comparisons. Calculations were performed by R version 3.6.1 and STATA 14.0. All steps were performed according to PRISMA guidelines.

Results: A total of 20 studies were enrolled in this network meta-analysis, providing 5,337 hepatocellular carcinoma cases and 6,585 controls. All included studies had an acceptable quality. Pairwise meta-analysis demonstrated that miR-196a2 rs11614913 was significantly associated with the susceptibility of HCC, while the other three SNPs were not found to have a significant association. In the analysis of HCC patients under different HBV infection status, only miR-196a2 revealed correlation of threefold risk. The network results showed no significant difference in the distribution of genotype frequencies except for miR-196a2, which appeared to have the highest superiority index when comparing and ranking four SNPs.

Conclusion: MiR-196a2 rs11614913 was significantly associated with the susceptibility of HCC, especially for HBV- related HCC, and that individuals with TC/CC were more susceptible. No significant association was found in the other three miRNA genes. MiR-196a2 could serve as the best predictor of susceptibility in HCC.
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http://dx.doi.org/10.3389/fonc.2020.562019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851082PMC
January 2021

Urinary Single-Cell Profiling Captures the Cellular Diversity of the Kidney.

J Am Soc Nephrol 2021 03 2;32(3):614-627. Epub 2021 Feb 2.

Renal, Electrolyte, and Hypertension Division, Department of Medicine, University of Pennsylvania, Perelman School of Medicine, Philadelphia, Pennsylvania

Background: Microscopic analysis of urine sediment is probably the most commonly used diagnostic procedure in nephrology. The urinary cells, however, have not yet undergone careful unbiased characterization.

Methods: Single-cell transcriptomic analysis was performed on 17 urine samples obtained from five subjects at two different occasions, using both spot and 24-hour urine collection. A pooled urine sample from multiple healthy individuals served as a reference control. In total 23,082 cells were analyzed. Urinary cells were compared with human kidney and human bladder datasets to understand similarities and differences among the observed cell types.

Results: Almost all kidney cell types can be identified in urine, such as podocyte, proximal tubule, loop of Henle, and collecting duct, in addition to macrophages, lymphocytes, and bladder cells. The urinary cell-type composition was subject specific and reasonably stable using different collection methods and over time. Urinary cells clustered with kidney and bladder cells, such as urinary podocytes with kidney podocytes, and principal cells of the kidney and urine, indicating their similarities in gene expression.

Conclusions: A reference dataset for cells in human urine was generated. Single-cell transcriptomics enables detection and quantification of almost all types of cells in the kidney and urinary tract.
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http://dx.doi.org/10.1681/ASN.2020050757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920183PMC
March 2021

extract and baicalein inhibit replication of SARS-CoV-2 and its 3C-like protease .

J Enzyme Inhib Med Chem 2021 Dec;36(1):497-503

BNLMS, Peking-Tsinghua Center for Life Sciences at College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

COVID-19 has become a global pandemic and there is an urgent call for developing drugs against the virus (SARS-CoV-2). The 3C-like protease (3CL) of SARS-CoV-2 is a preferred target for broad spectrum anti-coronavirus drug discovery. We studied the anti-SARS-CoV-2 activity of and its ingredients. We found that the ethanol extract of and its major component, baicalein, inhibit SARS-CoV-2 3CL activity with IC's of 8.52 µg/ml and 0.39 µM, respectively. Both of them inhibit the replication of SARS-CoV-2 in Vero cells with EC's of 0.74 µg/ml and 2.9 µM, respectively. While baicalein is mainly active at the viral post-entry stage, the ethanol extract also inhibits viral entry. We further identified four baicalein analogues from other herbs that inhibit SARS-CoV-2 3CL activity at µM concentration. All the active compounds and the extract also inhibit the SARS-CoV 3CL, demonstrating their potential as broad-spectrum anti-coronavirus drugs.
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http://dx.doi.org/10.1080/14756366.2021.1873977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850424PMC
December 2021

The Nexus between Economic Complexity and Energy Consumption under the Context of Sustainable Environment: Evidence from the LMC Countries.

Int J Environ Res Public Health 2020 12 27;18(1). Epub 2020 Dec 27.

School of Economics, Yunnan University, Kunming 650106, China.

The wide application of various energy resources in economic development is allegedly responsible for deepening environmental deterioration in terms of increasing pollution emissions and other negative consequences including climate change. This current work investigates the interdependent correlation between energy consumption (both fossil fuel energy consumption and renewable energy consumption) and economic complexity among Lancang-Mekong Cooperation (hereafter LMC) countries, from 1991 to 2017. As for empirical analysis, a panel vector autoregression (PVAR) model was employed. Outcomes of this research confirm the existence of a unidirectional relationship between energy consumption and economic complexity index. It is verified that renewable energy usage is a possible alternative to traditional energy and is able to increase economic complexity. This current research proposed to contribute as a pioneering exploration on LMC countries by adding original observations into existing studies. Finally, we will discuss policy implications of this work.
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http://dx.doi.org/10.3390/ijerph18010124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796423PMC
December 2020

Altered serum levels of IL-36 cytokines (IL-36α, IL-36β, IL-36γ, and IL-36Ra) and their potential roles in Guillain-Barré syndrome.

Medicine (Baltimore) 2020 Dec;99(52):e23832

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

Abstract: Guillain-Barré syndrome (GBS) is an acute autoimmune neurological disorder mainly involving the peripheral nerves. Currently, various cytokines have been shown to be involved in the pathogenesis of GBS. Because of their similar biological structures, interleukin (IL)-36α, IL-36β, IL-36γ, and IL-36 receptor antagonist (Ra) were all renamed and collectively called IL-36 cytokines. The roles of IL-36 cytokines in GBS currently remain unclear.Forty-two patients with GBS and 32 healthy volunteers were included in our study. Serum IL-36α, β, γ, and interleukin-36 receptor antagonist (IL-36Ra) levels of patients with GBS in the acute and remission phases and healthy volunteers were measured by enzyme-linked immunosorbent assay (ELISA). In addition, we examined the serum levels of other inflammatory factors that have been shown to be involved in GBS pathogenesis, represented by IL-17 and tumor necrosis factor-α (TNF-α). Furthermore, the correlations between the serum levels of IL-36 cytokines and different clinical data or the serum levels of other inflammatory factors in GBS patients were analyzed.Significantly higher serum IL-36α and IL-36γ levels were measured in the acute phase than in the remission phase and in healthy control (HC) subjects (P < .05), while lower serum IL-36Ra levels were measured in the acute phase than in the remission phase and in HC subjects (P < .05). Serum IL-36α and IL-36γ levels were positively correlated with GBS disability scale scores (GDSs), while serum IL-36Ra levels were negatively correlated with GDSs. Correlation analyses among inflammatory factors showed that serum IL-36α and IL-36γ levels in GBS patients were positively correlated with serum IL-17 and TNF-α levels, while serum IL-36Ra levels were negatively correlated with the levels of these 2 inflammatory factors. Similar results were observed in cerebrospinal fluid (CSF), IL-36α and IL-36γ levels in CSF were positively correlated with GDSs, while IL-36Ra levels in CSF were negatively correlated with GDSs. Additionally, the serum and CSF levels of IL-36α and IL-36γ in the axonal subtype of GBS patients were higher than those in the demyelination subtype.Based on our findings, IL-36 cytokines may be involved in the pathogenesis of GBS and some of these cytokines may help predict the disease severity and other clinical characteristics of GBS.
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http://dx.doi.org/10.1097/MD.0000000000023832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769357PMC
December 2020

Simultaneous recovery of ammonium and total phosphorus from toilet tail water by modified palygorskite-bentonite clay.

Water Environ Res 2021 Jul 26;93(7):1077-1086. Epub 2020 Dec 26.

School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai, China.

Suitable treatment of toilet sewage is a worldwide challenge. The anaerobic baffled reactor (ABR)-microbial fuel cell (MFC)-microbial electrolysis cell (MEC) (AMM) coupling treatment system has been constructed achieving effective removal of carbon, nitrogen, and phosphorus from toilet sewage and resource recovery; however, ammonium (NH -N) and total phosphorus (TP) accumulation in tail water is a found problem of the system. In this study, acid-modified and alkali-heat modified palygorskite-bentonite (Pal-Ben) were used to recover NH -N and TP from the AMM toilet tail water simultaneously. The higher adsorption capacity of the modified clay is attributed to the changes of surface structure of the material. The modified clay Pal-Ben (mass ratio 1:3) activated with alkali performed the highest NH -N and TP recovery rates of 83.6% and 85.5%, respectively. The adsorption of NH -N was more in line with the pseudo-second-order kinetic model and confirmed to be a chemical adsorption process, while the adsorption of TP was more in line with the pseudo-first-order kinetics and a physical adsorption process; the adsorption capacity of NH -N accelerated with decrease of TP removal when pH increased. This study developed a low cost and high capacity of alkaline thermally modified clay removing/recovering NH -N and TP from toilet tail water simultaneously. PRACTITIONER POINTS: A cheap composite clay was developed to recover nitrogen and phosphorus from toilet tail water simultaneously. The low costs and high capacity of alkaline thermally modified clay make it stand out in NH -N and TP removal of toilet tail water. The process mechanism of simultaneous nitrogen and phosphorus recovery was clarified with characterization and kinetic model fitting. The used clay loaded with nutrients could be applied as a slow-release compound fertilizer for soil improvement.
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http://dx.doi.org/10.1002/wer.1495DOI Listing
July 2021

The Nuclear Receptor ESRRA Protects from Kidney Disease by Coupling Metabolism and Differentiation.

Cell Metab 2021 02 9;33(2):379-394.e8. Epub 2020 Dec 9.

Renal, Electrolyte, and Hypertension Division, Department of Medicine, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA 19104, USA; Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA 19104, USA. Electronic address:

Kidney disease is poorly understood because of the organ's cellular diversity. We used single-cell RNA sequencing not only in resolving differences in injured kidney tissue cellular composition but also in cell-type-specific gene expression in mouse models of kidney disease. This analysis highlighted major changes in cellular diversity in kidney disease, which markedly impacted whole-kidney transcriptomics outputs. Cell-type-specific differential expression analysis identified proximal tubule (PT) cells as the key vulnerable cell type. Through unbiased cell trajectory analyses, we show that PT cell differentiation is altered in kidney disease. Metabolism (fatty acid oxidation and oxidative phosphorylation) in PT cells showed the strongest and most reproducible association with PT cell differentiation and disease. Coupling of cell differentiation and the metabolism was established by nuclear receptors (estrogen-related receptor alpha [ESRRA] and peroxisomal proliferation-activated receptor alpha [PPARA]) that directly control metabolic and PT-cell-specific gene expression in mice and patient samples while protecting from kidney disease in the mouse model.
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http://dx.doi.org/10.1016/j.cmet.2020.11.011DOI Listing
February 2021

Microencapsulation of sea buckthorn ( L.) pulp oil by spray drying.

Food Sci Nutr 2020 Nov 22;8(11):5785-5797. Epub 2020 Oct 22.

Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Shaanxi University of Chinese Medicine Xianyang China.

The aim of this work was to encapsulate sea buckthorn ( L.) pulp oil (SBPO) by spray drying. Gum Arabic (GA) and maltodextrins (MD) were used as wall materials. The effects of several factors, including GA to MD ratio, total solids content of emulsion, wall to core ratio, and inlet air temperature, on the microencapsulation efficiency (ME) were investigated. The optimization of operation conditions was realized by response surface methodology (RSM). The optimal conditions were as follows: GA to MD ratio 2.38, total solids content 39%, wall to core ratio 5.33, and inlet air temperature 154°C. Under the optimal conditions, the ME of SBPO microcapsules was 94.96 ± 1.42%. The physicochemical properties of microcapsules were also invested. SBPO microcapsules obtained had low water activity, low moisture content, high water solubility, and high bulk density. For the morphological characteristics, cracks and pores were not observed in most microcapsules, which was beneficial for the protection of ingredients in microcapsules. The particle size of SBPO microcapsules ranged from 0.01 to 5 μm, and the mean diameter was 2.55 μm. The analysis results of fourier transform infrared spectroscopy (FTIR) informed the presence of SBPO in microcapsules. There were no significant differences in the content of the main fatty acids in SBPO before and after spray drying. The results of oxidative stability showed that the microencapsulation by spray drying could effectively protect SBPO from oxidation and extend the shelf life of SBPO.
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http://dx.doi.org/10.1002/fsn3.1828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684620PMC
November 2020

Weak electricity stimulates biological nitrate removal of wastewater: Hypothesis and first evidences.

Sci Total Environ 2021 Feb 16;757:143764. Epub 2020 Nov 16.

Aix-Marseille Univ, CNRS, IRD, INRA, Coll France, CEREGE, 13100 Aix en Provence, France.

Nitrate pollution in water is a worldwide health and environmental concern. Biological nitrate removal of wastewater is widely used countering eutrophication of water bodies; however it could be troublesome and expensive when influent carbon source is insufficient. Here we present a novel process, the microbial fuel cell (MFC)-resistance-type electrical stimulation denitrification process (RtESD) using microbial weak electricity originated from the wastewater, to enhance nitrate removal. Results show that the optimal nitrate dependent denitrification rate (0.027 mg N/L·h) and nitrate removal efficiency (98.1%) can be achieved; partial autotrophic denitrification was enhanced in RtESD under stimulation of 0.2 V of microbial weak electricity (MWE). Aromatic proteins also increased in the presence of 0.2 V MWE stimulation according to three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy profiles, indicating that electron transfer could be improved in the case of MWE stimulation. Furthermore, the microbial community structure and diversity analysis results demonstrated that MWE stimulation inhibited the heterotrophic denitrifying bacteria and activated the autotrophic denitrifying bacteria in RtESD. Two hypotheses, enhancement of electron transfer and improvement of microorganism activity, were proposed regarding to the MWE stimulated pathways. This study provided a promising method utilizing MWE derived from wastewater to improve the denitrification rate and removal efficiency of nitrate-containing wastewater treatment processes.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143764DOI Listing
February 2021
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