Publications by authors named "Hongbo Liu"

446 Publications

Cytochrome P450 mono-oxygenase CYP703A2 plays a central role in sporopollenin formation and ms5ms6 fertility in cotton.

J Integr Plant Biol 2022 Aug 5. Epub 2022 Aug 5.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

The double-recessive genic male-sterile (ms) line ms5 ms6 has been used to develop cotton (Gossypium hirsutum) hybrids for many years, but its molecular-genetic basis has remained unclear. Here, we identified the Ms5 and Ms6 loci through map-based cloning and confirmed their function in male sterility through CRISPR/Cas9 gene editing. Ms5 and Ms6 are highly expressed in stage 7-9 anthers and encode the cytochrome P450 mono-oxygenases CYP703A2-A and CYP703A2-D. The ms5 mutant carries a single-nucleotide C-to-T nonsense mutation leading to premature chain termination at amino acid 312 (GhCYP703A2-A ), and ms6 carries three non-synonymous substitutions (D98E, E168K, and G198R) and a synonymous mutation (L11L). Enzyme assays showed that GhCYP703A2 proteins hydroxylate fatty acids, and the ms5 (GhCYP703A2-A ) and ms6 (GhCYP703A2-D ) mutant proteins have decreased enzyme activities. Biochemical and lipidomic analyses showed that in ms5 ms6 plants, C12-C18 free fatty acid and phospholipid levels are significantly elevated in stage 7-9 anthers, while stage 8-10 anthers lack sporopollenin fluorescence around the pollen, causing microspore degradation and male sterility. Overall, our characterization uncovered functions of GhCYP703A2 in sporopollenin formation and fertility, providing guidance for creating male-sterile lines to facilitate hybrid cotton production and thus exploit heterosis for improvement of cotton. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13340DOI Listing
August 2022

Exosomes Derived from Baicalin-Pretreated Mesenchymal Stem Cells Alleviate Hepatocyte Ferroptosis after Acute Liver Injury via the Keap1-NRF2 Pathway.

Oxid Med Cell Longev 2022 21;2022:8287227. Epub 2022 Jul 21.

Department of Infectious Disease, The Affiliated Fuyang Hospital of Bengbu Medical College, Fuyang People's Hospital, Fuyang, 236000 Anhui, China.

Acute liver injury (ALI) is characterized as a severe metabolic dysfunction caused by extensive damage to liver cells. Ferroptosis is a type of cell death dependent on iron and oxidative stress, which differs from classical cell death, such as apoptosis and necrosis. Ferroptosis has unique morphological features, which mainly include mitochondrial dissolution and mitochondrial outline reduction. Furthermore, the intracellular accumulation of lipid peroxides directly affects the occurrence of ferroptosis. Baicalin, the main compound isolated from , has anti-inflammatory and antioxidative effects. Recently, exosomes derived from preconditioned mesenchymal stem cells (MSCs) have shown great potential in the treatment of various diseases including ALI. This study investigates the ability of exosomes derived from baicalin-pretreated MSCs (Ba-Exo) to promote liver function recovery in mice with ALI compared with those without pretreatment. Through in vivo and in vitro experiments, this study demonstrates for the first time that Ba-Exo greatly attenuates D-galactosamine and lipopolysaccharide (D-GaIN/LPS)-induced liver damage and inhibits reactive oxygen species (ROS) production and lipid peroxide-induced ferroptosis. Moreover, P62 was significantly upregulated in Ba-Exo, whereas its downregulation in Ba-Exo counteracted the beneficial effect of Ba-Exo. P62 regulates hepatocyte ferroptosis by activating the Keap1-NRF2 pathway. The beneficial effect of Ba-Exo in inhibiting ferroptosis was also attenuated after the NRF2 pathway was inhibited. Therefore, baicalin pretreatment is an effective and promising approach to optimize the therapeutic efficacy of MSC-derived exosomes in ALI.
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http://dx.doi.org/10.1155/2022/8287227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334037PMC
August 2022

Rapid PCR-Based Nanopore Adaptive Sequencing Improves Sensitivity and Timeliness of Viral Clinical Detection and Genome Surveillance.

Front Microbiol 2022 16;13:929241. Epub 2022 Jun 16.

Academy of Military Medical Sciences, Academy of Military Sciences, Beijing, China.

Nanopore sequencing has been widely used for the real-time detection and surveillance of pathogens with portable MinION. Nanopore adaptive sequencing can enrich on-target sequences without additional pretreatment. In this study, the performance of adaptive sequencing was evaluated for viral genome enrichment of clinical respiratory samples. Ligation-based nanopore adaptive sequencing (LNAS) and rapid PCR-based nanopore adaptive sequencing (RPNAS) workflows were performed to assess the effects of enrichment on nasopharyngeal swab samples from human adenovirus (HAdV) outbreaks. RPNAS was further applied for the enrichment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from nasopharyngeal swab samples to evaluate sensitivity and timeliness. The RPNAS increased both the relative abundance (7.87-12.86-fold) and data yield (1.27-2.15-fold) of HAdV samples, whereas the LNAS increased only the relative abundance but had no obvious enrichment on the data yield. Compared with standard nanopore sequencing, RPNAS detected the SARS-CoV-2 reads from two low-abundance samples, increased the coverage of SARS-CoV-2 by 36.68-98.92%, and reduced the time to achieve the same coverage. Our study highlights the utility of RPNAS for virus enrichment directly from clinical samples, with more on-target data and a shorter sequencing time to recover viral genomes. These findings promise to improve the sensitivity and timeliness of rapid identification and genomic surveillance of infectious diseases.
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http://dx.doi.org/10.3389/fmicb.2022.929241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244360PMC
June 2022

Epidemiological and etiological characteristics of viral meningitis for hospitalized pediatric patients in Yunnan, China.

Medicine (Baltimore) 2022 Jul 1;101(26):e29772. Epub 2022 Jul 1.

Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, PR China.

Background: Viral infection is the most common cause of aseptic meningitis. The purpose of this study was to identify the viruses responsible for aseptic meningitis to better understand the clinical presentations of this disease.

Method: Between March 2009 and February 2010, we collected 297 cerebrospinal fluid specimens from children with aseptic meningitis admitted to a pediatric hospital in Yunnan (China). Viruses were detected by using "in house" real-time quantitative polymerase chain reaction or reverse-transcription real-time quantitative polymerase chain reaction from these samples. Phylogenetic analyses were conducted using the Molecular Evolutionary Genetic Analysis version 7.0 software, with the neighbor-joining method.

Results: Viral infection was diagnosed in 35 of the 297 children (11.8%). The causative viruses were identified to be enteroviruses in 25 cases (71.4%), varicella-zoster virus in 5 cases (14.3%), herpes simplex virus 1 in 2 cases (5.7%), and herpes simplex virus 2, Epstein-Barr virus, and human herpesvirus 6 in 1 case each (2.9% each). Of the enteroviruses, coxsackievirus B5 was the most frequently detected serotype (10/25 cases; 40.0%) and all coxsackievirus B5 strains belonged to C group.

Conclusions: In the study, a causative virus was only found in the minority of cases, of them, enteroviruses were the most frequently detected viruses in patients with viral meningitis, followed by varicella-zoster virus and herpes simplex virus. Our findings underscore the need for enhanced surveillance and etiological study of aseptic meningitis.
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http://dx.doi.org/10.1097/MD.0000000000029772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239644PMC
July 2022

Genome-wide meta-analysis and omics integration identifies novel genes associated with diabetic kidney disease.

Diabetologia 2022 Sep 28;65(9):1495-1509. Epub 2022 Jun 28.

Programs in Metabolism and Medical & Population Genetics, Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Aims/hypothesis: Diabetic kidney disease (DKD) is the leading cause of kidney failure and has a substantial genetic component. Our aim was to identify novel genetic factors and genes contributing to DKD by performing meta-analysis of previous genome-wide association studies (GWAS) on DKD and by integrating the results with renal transcriptomics datasets.

Methods: We performed GWAS meta-analyses using ten phenotypic definitions of DKD, including nearly 27,000 individuals with diabetes. Meta-analysis results were integrated with estimated quantitative trait locus data from human glomerular (N=119) and tubular (N=121) samples to perform transcriptome-wide association study. We also performed gene aggregate tests to jointly test all available common genetic markers within a gene, and combined the results with various kidney omics datasets.

Results: The meta-analysis identified a novel intronic variant (rs72831309) in the TENM2 gene associated with a lower risk of the combined chronic kidney disease (eGFR<60 ml/min per 1.73 m) and DKD (microalbuminuria or worse) phenotype (p=9.8×10; although not withstanding correction for multiple testing, p>9.3×10). Gene-level analysis identified ten genes associated with DKD (COL20A1, DCLK1, EIF4E, PTPRN-RESP18, GPR158, INIP-SNX30, LSM14A and MFF; p<2.7×10). Integration of GWAS with human glomerular and tubular expression data demonstrated higher tubular AKIRIN2 gene expression in individuals with vs without DKD (p=1.1×10). The lead SNPs within six loci significantly altered DNA methylation of a nearby CpG site in kidneys (p<1.5×10). Expression of lead genes in kidney tubules or glomeruli correlated with relevant pathological phenotypes (e.g. TENM2 expression correlated positively with eGFR [p=1.6×10] and negatively with tubulointerstitial fibrosis [p=2.0×10], tubular DCLK1 expression correlated positively with fibrosis [p=7.4×10], and SNX30 expression correlated positively with eGFR [p=5.8×10] and negatively with fibrosis [p<2.0×10]).

Conclusions/interpretation: Altogether, the results point to novel genes contributing to the pathogenesis of DKD.

Data Availability: The GWAS meta-analysis results can be accessed via the type 1 and type 2 diabetes (T1D and T2D, respectively) and Common Metabolic Diseases (CMD) Knowledge Portals, and downloaded on their respective download pages ( https://t1d.hugeamp.org/downloads.html ; https://t2d.hugeamp.org/downloads.html ; https://hugeamp.org/downloads.html ).
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http://dx.doi.org/10.1007/s00125-022-05735-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345823PMC
September 2022

Prediction of the generalization of myasthenia gravis with purely ocular symptoms at onset: a multivariable model development and validation.

Ther Adv Neurol Disord 2022 21;15:17562864221104508. Epub 2022 Jun 21.

Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.

Background: About half of myasthenia gravis (MG) patients with purely ocular symptoms at onset progress to generalized myasthenia gravis (gMG).

Objectives: To develop and validate a model to predict the generalization of MG at 6 months after disease onset in patients with ocular-onset myasthenia gravis (OoMG).

Methods: Data of patients with OoMG were retrospectively collected from two tertiary hospitals in Germany and China. An accelerated failure time model was developed using the backward elimination method based on the German cohort to predict the generalization of OoMG. The model was then externally validated in the Chinese cohort, and its performance was assessed using Harrell's C-index and calibration plots.

Results: Four hundred and seventy-seven patients (275 from Germany and 202 from China) were eligible for inclusion. One hundred and three (37.5%) patients in the German cohort progressed from OoMG to gMG with a median follow-up time of 69 (32-116) months. The median time to generalization was 29 (16-71) months. The estimated cumulative probability of generalization was 30.5% [95% CI (confidence interval), 24.3-36.2%) at 5 years after disease onset. The final model, which was represented as a nomogram, included five clinical variables: sex, titer of anti-AChR antibody, status of anti-MuSK antibody, age at disease onset and the presence of other autoimmune disease. External validation of the model using the bootstrap showed a C-index of 0.670 (95% CI, 0.602-0.738). Calibration curves revealed moderate agreement of predicted and observed outcomes.

Conclusion: The nomogram is a good predictor for generalization in patients with OoMG that can be used to inform of the individual generalization risk, which might improve the clinical decision-making.
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http://dx.doi.org/10.1177/17562864221104508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218496PMC
June 2022

Effect of Boron Doping on the Interlayer Spacing of Graphite.

Materials (Basel) 2022 Jun 13;15(12). Epub 2022 Jun 13.

College of Materials Science and Engineering, Hunan University, Changsha 410082, China.

Boron-doped graphite was prepared by the heat treatment of coke using BC powder as a graphitization catalyst to investigate the effects of the substitutional boron atoms on the interlayer spacing of graphite. Boron atoms can be successfully incorporated into the lattice of graphite by heat treatment, resulting in a reduction in the interlayer spacing of graphite to a value close to that of ideal graphite (0.3354 nm). With an increase in the catalyst mass ratio, the content of substituted boron in the samples increased significantly, causing a decrease in the interlayer spacing of the boron-doped graphite. Density functional theory calculations suggested that the effects of the substitutional boron atoms on the interlayer spacing of the graphite may be attributed to the transfer of Π electrons between layers, the increase in the electrostatic surface potential of the carbon layer due to the electron-deficient nature of boron atoms, and Poisson contraction along the c-axis.
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http://dx.doi.org/10.3390/ma15124203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229557PMC
June 2022

Identification of a novel c.259G > T mutation in the ABO*B.01 allele giving rise to a B phenotype.

Transfusion 2022 Jul 17;62(7):E34-E36. Epub 2022 Jun 17.

Clinical Research Center and Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

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http://dx.doi.org/10.1111/trf.16948DOI Listing
July 2022

Epigenomic and transcriptomic analyses define core cell types, genes and targetable mechanisms for kidney disease.

Nat Genet 2022 Jul 16;54(7):950-962. Epub 2022 Jun 16.

Department of Medicine, Renal Electrolyte and Hypertension Division, University of Pennsylvania, Philadelphia, PA, USA.

More than 800 million people suffer from kidney disease, yet the mechanism of kidney dysfunction is poorly understood. In the present study, we define the genetic association with kidney function in 1.5 million individuals and identify 878 (126 new) loci. We map the genotype effect on the methylome in 443 kidneys, transcriptome in 686 samples and single-cell open chromatin in 57,229 kidney cells. Heritability analysis reveals that methylation variation explains a larger fraction of heritability than gene expression. We present a multi-stage prioritization strategy and prioritize target genes for 87% of kidney function loci. We highlight key roles of proximal tubules and metabolism in kidney function regulation. Furthermore, the causal role of SLC47A1 in kidney disease is defined in mice with genetic loss of Slc47a1 and in human individuals carrying loss-of-function variants. Our findings emphasize the key role of bulk and single-cell epigenomic information in translating genome-wide association studies into identifying causal genes, cellular origins and mechanisms of complex traits.
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http://dx.doi.org/10.1038/s41588-022-01097-wDOI Listing
July 2022

Processed product (Pinelliae Rhizoma Praeparatum) of Pinellia ternata (Thunb.) Breit. Alleviates the allergic airway inflammation of cold phlegm via regulation of PKC/EGFR/MAPK/PI3K-AKT signaling pathway.

J Ethnopharmacol 2022 Sep 7;295:115449. Epub 2022 Jun 7.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing, 210023, China; State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. Electronic address:

Ethnopharmacological Relevance: Pinelliae Rhizoma Praeparatum (PRP) is a traditional processed product of Pinellia ternata (Thunb.) Berit., which mainly used for treating cold asthma (CA). However, the mechanism of action of PRP for treating CA have not been fully elucidated.

Aim Of The Study: To investigate the core active constituents and the pharmacological mechanism of PRP against CA.

Materials And Methods: Ovalbumin (OVA) and cold water-induced cold asthma model were established in male mice. The effects of water extract from PRP were evaluated by general morphological observation, expectorant activity, airway hyperresponsiveness, mucus hypersecretion, inflammatory cytokines, etc. Additionally, the mRNA and protein expression of mucin 5AC (MUC5AC) and aquaporin 5 (AQP5) in vivo and in vitro were detected by immunohistochemistry (IHC), qRT-PCR, and western blotting. The mechanisms of action were investigated through network pharmacology and transcriptomic, and validated through western blotting and molecular docking.

Results: PRP exhibited a favorable expectorant activity, and significantly reduced the airway inflammation, mucus secretion, and hyperresponsiveness in cold asthma model. It also reduced the levels of IL-4, IL-5, IL-8, and IL-13 in bronchoalveolar lavage fluid (BALF) and IL-4 and total IgE in serum, while obviously increased the levels of IL-10 and IFN-γ in serum for asthmatic mice. Meanwhile, PRP also attenuated the pathological changes and mucus production in cold asthmatic mice. Moreover, the downregulation of MUC5AC and upregulation of AQP 5 were detected by western blotting and qRT-PCR after administration with PRP both in vivo and in vitro. PRP expectedly inhibited the protein expression of PKC-α, SRC, p-EGFR, p-ERK1/2, p-JNK, p-p38, p-PI3K, and p-Akt levels in vivo.

Conclusions: These combined data showed that PRP suppressed the allergic airway inflammation of CA by regulating the balance of Th1 and Th2 cytokines and the possible involvement of the PKC/EGFR/MAPK/PI3K-Akt signaling pathway. Pentadecanoic acid, licochalcone A, β-sitosterol, etc. were considered as main active ingredients of PRP against CA. This study provides a novel perspective of the classical herbal processed product PRP in the treatment of CA.
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http://dx.doi.org/10.1016/j.jep.2022.115449DOI Listing
September 2022

Transference Number Reinforced-Based Gel Copolymer Electrolyte for Dendrite-Free Lithium Metal Batteries.

ACS Appl Mater Interfaces 2022 May 31. Epub 2022 May 31.

Shenzhen Key Laboratory of Power Battery Safety and Shenzhen Geim Graphene Center Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.

The progress of electric vehicles is highly inhibited by the limited energy density and growth of dendrite Li in current power batteries. Breakthroughs and improvements in electrolyte chemistry are highlighted to directly address the above issues, namely, the development of electrolytes with a high lithium-ion transference number (), enabling one to effectively restrict the concentration polarization during repetitious cycling. Herein, we propose a novel ether-based copolymer-based gel polymer electrolyte (ECP-based GPE) by in situ copolymerization as an intriguing strategy to achieve a high of ∼0.64. Molecular dynamics simulations and finite element method analyses illustrate the enhanced Li diffusion process (, ∼1.76 × 10 m s) in ECP-based GPE with a homogeneous electric potential accommodated around the lithium metal anode. Therefore, such a high--based electrolyte renders a high reversibility of dendrite-free lithium plating/stripping at a high areal capacity (5 mA cm/5 mA h cm) in an Li||Li symmetric cell and facilitates superior cycling performances (over 1000 cycles) at a high rate (5 C) with a capacity retention of ∼91.1% in Li||LiFePO batteries, promoting the practical application of solid-state lithium metal batteries.
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http://dx.doi.org/10.1021/acsami.2c01513DOI Listing
May 2022

Potassium ferrate pretreatment promotes short chain fatty acids yield and antibiotics reduction in acidogenic fermentation of sewage sludge.

J Environ Sci (China) 2022 Oct 10;120:41-52. Epub 2022 Jan 10.

School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address:

During the acidogenic fermentation converting waste activated sludge (WAS) into short-chain fatty acids (SCFA), hydrolysis of complex organic polymers is a limiting step and the transformation of harmful substances (such as antibiotics) during acidogenic fermentation is unknown. In this study, potassium ferrate (KFeO) oxidation was used as a pretreatment strategy for WAS acidogenic fermentation to increase the hydrolysis of sludge and destruct the harmful antibiotics. Pretreatment with KFeO can effectively increase the SCFA production during acidogenic fermentation and change the distribution of SCFA components. With the dosage of 0.2 g/g TS, the maximum SCFA yield was 4823 mg COD/L, which is 28.3 times that of the control group; acetic acid accounts for more than 90% of the total SCFA. The higher dosage (0.5 g/g TS) can further increase the proportion of acetic acid, but inhibit the overall performance of SCFA production. Apart from the promotion of hydrolysis and acidogenesis, KFeO pretreatment can also simultaneously oxidizes and degrades part of the antibiotics in the sludge. When the dosage is 0.5 g/g TS, the degradation efficacy of antibiotics is the most significant, and the contents of ofloxacin, azithromycin, and tetracycline in the sludge are reduced by 69%, 42%, and 50%, respectively. In addition, KFeO pretreatment can also promote the release of antibiotics from sludge flocs, which is conducive to the simultaneous degradation of antibiotics in the subsequent biological treatment process.
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http://dx.doi.org/10.1016/j.jes.2022.01.001DOI Listing
October 2022

Multi-mineral fingerprinting analysis of the Chinese mitten crab (Eriocheir sinensis) in Yangcheng Lake during the year-round culture period.

Food Chem 2022 Oct 7;390:133167. Epub 2022 May 7.

Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. Electronic address:

Chinese mitten crabs (Eriocheir sinensis), originating from Yangcheng Lake, are valuable aquatic products in China. To characterize changes in nutrients in this species in Yangcheng Lake during the year-round culture period, the contents of ten mineral elements in the third pereiopod were evaluated. Principal component analysis revealed that mineral elements changed substantially in the first three months. Thereafter, the elemental "fingerprint" stabilized, and samples could not be accurately distinguished. This pattern was supported by linear discriminant analysis and self-organizing map analysis. These results demonstrate that a long period of time is required for element characteristics to stabilize, suggesting that short-term breeding is insufficient to obtain the natural elemental "fingerprint." In addition, our findings provide a basis for verifying the origin of Chinese mitten crab and other aquatic taxa in Yangcheng Lake.
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http://dx.doi.org/10.1016/j.foodchem.2022.133167DOI Listing
October 2022

The role of microbiome in carbon sequestration and environment security during wastewater treatment.

Sci Total Environ 2022 Sep 10;837:155793. Epub 2022 May 10.

State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China.

Wastewater treatment is an essential aspect of the earth's sustainable future. However, different wastewater treatment methods are responsible for carbon discharge into the environment, raising environmental risks. Hence, such wastewater treatment methods are required that can minimize carbon release without compromising the treatment quality. Microbiome-based carbon sequestration is a potential method for achieving this goal. Limited studies have been carried out to investigate how microbes can capture and utilize CO. This review summarizes the approaches including microbial electrolytic carbon capture, microbial electrosynthesis, microbial fuel cell, microalgae cultivation, and constructed wetlands that employ microbes to capture and utilize CO. Electroactive Bacteria (EAB) convert carbon dioxide to carbonates and bicarbonates in subsequent steps after organic matter decomposition. Similarly, microbial electrosynthesis (MES) not only helps capture carbon but also produces secondary products (production of polyhydroxyalkanoates by Gram-negative rod Aeromonas hydrophila bacteria) of commercial importance during wastewater treatment. In addition to this, microbial carbon capture cells (MCCs) have been now utilized for energy generation and carbon sequestration at the same time during wastewater treatment. Moreover, microalgae cultivation has also been found to capture CO at a rapid pace while releasing O as a consequence of photosynthesis. Hence, microbe-based wastewater treatment has quite a potential due to two-fold benefits like carbon sequestration and by-product formation.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155793DOI Listing
September 2022

Promoted Electron Transfer and Surface Absorption by Single Nickel Atoms for Photocatalytic Cross-Coupling of Aromatic Alcohols and Aliphatic Amines under Visible Light.

ACS Appl Mater Interfaces 2022 Apr 15;14(16):18383-18392. Epub 2022 Apr 15.

College of Material Science and Engineering, Hunan University, Hunan, Changsha 410082, P. R. China.

The preparation of imines has drawn increasing attention as they are fundamental intermediates in the production of pharmaceuticals, agricultures, and fine chemicals. Nevertheless, current approaches for imines synthesis mainly focus on thermally driven reactions which always involve the consumption of high price noble metal catalysts, expensive ligands, strong base, and harsh reaction conditions. Herein, we demonstrate single atom nickel anchored on polymeric carbon nitride ([email protected]) in Ni-N structure for visible light-promoted crossed coupling between aromatic alcohols and aliphatic amines. As expected, the Ni atoms dispersed carbon nitride demonstrates an obviously improved charge separation and transfer as reflected in UV-vis, fluorescence intensity and lifetime, photocurrent density, and electrochemical impedance characterizations. More impressively, the density functional theory (DFT) calculations also reveals that the presence of Ni atoms can dramatically accelerate the absorption of reactive substrates on the surface of PCN. The decreased absorption energy from -0.51 to -3.35 eV, associated with increased O═O bond length from 1.226 to 1.371 Å indicates a huge advantage of single Ni atom on oxygen activation. As a result, the obtained [email protected] photocatalyst shows a prominent catalytic efficiency in imines formation with a reaction conversion of 73% and selectivity of >99%. Lastly, the photocatalytic reactions displays an excellent compatibility with various imines being achieved with high yield.
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http://dx.doi.org/10.1021/acsami.2c00311DOI Listing
April 2022

System dynamics-based prediction of municipal solid waste generation in high-cold and high-altitude area: The case of Lhasa, Tibet.

Waste Manag Res 2022 Apr 15:734242X221084077. Epub 2022 Apr 15.

School of Environmental Science and Engineering, Tianjin University, Tianjin, P.R. China.

The ecological environment in high-cold and high-altitude area is fragile and sensitive, which raise higher claim for municipal solid waste (MSW) management. In the high-cold and high-altitude area, there are problems, such as the mismatch between the actual amount of MSW generated and the scale of transportation and treatment facilities, and the inefficiency of MSW management. In terms of MSW forecasting methods, it is also difficult to forecast due to the lack of data. This study is the first to propose a system dynamics-based method for predicting the amount of MSW generated in high-cold and high-altitude area, and apply it to Lhasa. The research results show that the total amount of MSW generated in Lhasa is small, but the growth rate is fast. Through dynamic simulation, it is found that the synergistic consideration of gross domestic product (GDP) growth rate, urban construction policy and tourism development policy can significantly reduce the growth trend (14% emission reduction in 2030). In addition, strengthening supervision and restraint, publicity and education in high-cold and high-altitude area can produce better waste sorting effects, minimise the pressure on treatment facilities, and improve resource utilisation. Finally, the policy implications are suggested, for example, in the process of MSW management, the impact of economy, urbanisation, tourism and so on, should be taken into account and comprehensively adjusted. It is anticipated that this model and policy implications can be applied to other high-cold and high-altitude cities to provide data support and policy reference for the whole-process management of MSW.
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http://dx.doi.org/10.1177/0734242X221084077DOI Listing
April 2022

Effectiveness of BNT162b2 and mRNA-1273 Vaccines against COVID-19 Infection: A Meta-Analysis of Test-Negative Design Studies.

Vaccines (Basel) 2022 Mar 18;10(3). Epub 2022 Mar 18.

Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.

Although numerous COVID-19 vaccines are effective against COVID-19 infection and variants of concern (VOC) in the real world, it is imperative to obtain evidence of the corresponding vaccine effectiveness (VE). This study estimates the real-world effectiveness of the BNT162b2 and mRNA-1273 vaccines against COVID-19 infection and determines the influence of different virus variants on VE by using test-negative design (TND) studies. We systematically searched for published articles on the efficacy of BNT162b2 and mRNA-1273 against COVID-19 infection. Two researchers independently selected and extracted data from eligible studies. We calculated the VE associated with different vaccine types, SARS-CoV-2 variants, and vaccination statuses, using an inverse variance random-effects model. We selected 19 eligible studies in the meta-analysis from 1651 records. For the partially vaccinated group, the VE of BNT162b2 and mRNA-1273 was 61% and 78% against COVID-19 infection, respectively. For the completely vaccinated group, the VE of BNT162b2 and mRNA-1273 was 90% and 92% against COVID-19 infection, respectively. During subgroup analyses, the overall VE of BNT162b2 and mRNA-1273 against the Delta variant was 53% and 71%, respectively, for the partially vaccinated group; the respective VE values were 85% and 91% for the fully vaccinated group. Irrespective of the BNT162b2 or mRNA-1273 vaccines, the Delta variant significantly weakened vaccine protection for the partially vaccinated group, while full vaccination was highly effective against COVID-19 infection and various VOC. The mRNA-1273 vaccine is more effective against COVID-19 infection and VOC than the BNT162b2 vaccine, especially for the partially vaccinated group. Overall, the results provide recommendations for national and regional vaccine policies.
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http://dx.doi.org/10.3390/vaccines10030469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954872PMC
March 2022

A Study of the Mechanism and Separation of Structurally Similar Phenolic Acids by Commercial Polymeric Ultrafiltration Membranes.

Membranes (Basel) 2022 Mar 1;12(3). Epub 2022 Mar 1.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China.

This study examined the behavior and penetration mechanisms of typical phenolic (benzoic) acids, which determine their observed penetration rates during membrane separation, focusing on the influence of electrostatic and hydrophobic solute/membrane interactions. To understand the effects of hydrophobicity and electrostatic interaction on membrane filtration, the observed penetration of five structurally similar phenolic acids was compared with regenerated cellulose (RC) and polyamide (PA) membranes at different solute concentrations and solution pHs. Variation partitioning analysis (VPA) was performed to calculate the relative contributions of electrostatic and hydrophobic effects. The penetration of phenolic acids was mainly influenced by the electrostatic interaction, with salicylic acid having the highest penetration. Penetration of phenolic acids through the PA membrane decreased from 98% at pH 3.0 to 30-50% at pH 7.4, indicating the dominance of the electrostatic interaction. Moreover, based on its hydrophobicity and greater surface charge, the PA membrane could separate binary mixtures of protocatechuic/salicylic acid and 4-hydroxybenzoic/salicylic acid at pH 9.0, with separation factors of 1.81 and 1.78, respectively. These results provide a greater understanding of solute/membrane interactions and their effect on the penetration of phenolic acids through polymeric ultrafiltration membranes.
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http://dx.doi.org/10.3390/membranes12030285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950078PMC
March 2022

Discussion on the Rise of Healthy Chinese Sports Industry and the Training Mode of Compound Sports Talents.

Authors:
Hongbo Liu Ming Li

J Healthc Eng 2022 7;2022:6943285. Epub 2022 Mar 7.

School of Leisure Sports, Xi'an Physical Education University, Xi'an, Shaanxi 710068, China.

With the improvement of people's living standards and quality, sports charm stimulates people's awareness of sports consumption and makes sports industry develop rapidly into one of the important industries for the sustainable development of national economy. In modern society, sports industry has become a new industry with huge business opportunities. Especially, when the social economy develops to a certain level and sports form a certain scale, the interdependence, mutual support, and mutual promotion between sports and economy become more inseparable. This paper studies and discusses the training strategy of "compound" talents in sports industry under the background of healthy China strategy, in order to promote the development and innovation of China's sports industry. In terms of training sports talents, we should not only pay attention to personal technical, professional, practical, and understanding contents but also introduce some humanistic contents and social hot contents to improve the comprehensive quality of talents.
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http://dx.doi.org/10.1155/2022/6943285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920676PMC
May 2022

Increased blastomere number is associated with higher live birth rate in day 3 embryo transfer.

BMC Pregnancy Childbirth 2022 Mar 11;22(1):198. Epub 2022 Mar 11.

Center for Reproductive Medicine, Jiangxi Maternal and Child Health Hospital, Nanchang University School of Medicine, 318 Bayi Avenue, Nanchang, 330006, China.

Purpose: To study the relationship between blastomere number and pregnancy outcomes of day 3 embryo transfers.

Methods: This retrospective cohort study included 2237 fresh single day 3 embryo transfer cycles from October 2013 to November 2020. Patients were divided into six groups according to the blastomere number on day 3: ≤ 6-cell (n = 100), 7-cell (n = 207), 8-cell (n = 1522), 9-cell (n = 187), 10-cell (n = 91) and ≥ 11-cell (n = 130). Generalized estimating equation analysis based on multivariate logistic regression model was performed to adjust for potential confounders.

Results: The live birth rate (LBR) was 19.0%, 27.1%, 38.9%, 32.1%, 44.0% and 53.8% for the ≤ 6-cell, 7-cell, 8-cell, 9-cell, 10-cell and ≥ 11-cell groups, respectively (P < 0.001). Specifically, the ≤ 6-cell group was associated with reduced LBR compared with the 8-cell group (aOR 0.50, 95% CI 0.29-0.86; P = 0.013). Conversely, the odds of live birth were significantly increased in patients transferred with 10-cell embryos (aOR 1.62, 95% CI 1.03-2.53; P = 0.035) and ≥ 11-cell embryos (aOR 2.14, 95% CI 1.47-3.11; P < 0.001) when using the 8-cell embryo group as reference. Similar trends were also observed in the rates of positive hCG test and clinical pregnancy, while no significant differences were detected in miscarriage risk.

Conclusion: Increased blastomere number was associated with higher LBR in fresh single day 3 embryo transfer cycles. This finding questions the consensus on the reduced developmental potential of fast-cleaving embryos. Further large prospective studies are warranted for confirmation.
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http://dx.doi.org/10.1186/s12884-022-04521-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8917733PMC
March 2022

Contrast-free MRI quantitative parameters for early prediction of pathological response to neoadjuvant chemotherapy in breast cancer.

Eur Radiol 2022 Aug 10;32(8):5759-5772. Epub 2022 Mar 10.

Department of Radiology, The First Affiliated Hospital of China Medical University, No. 155, Nanjing Street, Heping District, Shenyang City, 110001, Liaoning Province, China.

Objectives: To assess early changes in synthetic relaxometry after neoadjuvant chemotherapy (NAC) for breast cancer and establish a model with contrast-free quantitative parameters for early prediction of pathological response.

Methods: From March 2019 to January 2021, breast MRI were performed for a primary cohort of women with breast cancer before (n = 102) and after the first (n = 93) and second (n = 90) cycle of NAC. Tumor size, synthetic relaxometry (T1/T2 relaxation time [T1/T2], proton density), and ADC were obtained, and the changes after treatment were calculated. Prediction models were established by multivariate logistic regression; evaluated with discrimination, calibration, and clinical application; and compared with Delong tests, net reclassification (NRI), and integrated discrimination index (IDI). External validation was performed from February to June 2021 with an independent cohort of 35 patients.

Results: In the primary cohort, all parameters changed after early treatment. Synthetic relaxometry decreased to a greater degree in major histologic responders (MHR, Miller-Payne G4-5) compared with non-MHR (Miller-Payne G1-3). A model combining ADC after treatment, changes in T1 and tumor size, and cancer subtype achieved the highest AUC after the first (primary/validation cohort, 0.83/0.82) and second cycles (primary/validation cohort, 0.85/0.84). No difference of AUC (p ≥ 0.27), NRI (p ≥ 0.31), and IDI (p ≥ 0.32) was found between models with different cycles and size-measured sequences. Model calibration and decision curves demonstrated a good fitness and clinical benefit, respectively.

Conclusions: Early reduction in synthetic relaxometry indicated pathological response to NAC. Contrast-free T1 and ADC combined with size and cancer subtype predicted effectively pathological response after one NAC cycle.

Key Points: • Synthetic MRI relaxometry changed after early neoadjuvant chemotherapy, which demonstrated pathological response for mass-like breast cancers. • Contrast-free quantitative parameters including T1 relaxation time and apparent diffusion coefficient, combined with tumor size and cancer subtype, stratified major histologic responders. • A contrast-free model predicted an early pathological response after the first treatment cycle of neoadjuvant chemotherapy.
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http://dx.doi.org/10.1007/s00330-022-08667-wDOI Listing
August 2022

Heavy metal accumulation in the surrounding areas affected by mining in China: Spatial distribution patterns, risk assessment, and influencing factors.

Sci Total Environ 2022 Jun 19;825:154004. Epub 2022 Feb 19.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, East Beijing Road 71, Nanjing 210008, China; University of Chinese Academy of Sciences, Yuquan Road 19, Beijing 100049, China.

Previous studies about heavy metal (HM) accumulation in the surrounding areas affected by mining mainly focused on a single or just a few mining areas. However, these studies could not provide adequate information supporting HM controls in soils at the national scale. This study first conducted a literature investigation and collected HM data in mining areas in China from 263 pieces of published literature. Then, geo-accumulation index (I), ecological risk index (ER), and health risk assessment model were adopted to evaluate their HM pollution, ecological risks, and health risks, respectively. Finally, Geodetector and Pearson correlation coefficients were used to explore the relationships between the spatial distribution patterns of HMs in soils and their influencing factors. Results showed that: (i) the average concentrations of Cd, Hg, Pb, Zn, Cu, As, Ni, and Cr were 5.4, 1.2, 335.3, 496.1, 105.8, 55.0, 42.6, and 72.4 mg kg, respectively, in the surrounding areas affected by mining in China; Cd pollution in soils (I = 2.9) was most severe; Cd (ER > 320) and Hg (ER > 320) were the main ecological risk factors; (ii) among the selected factors, mine types, clay content, soil organic carbon, and precipitation with the highest relative importance for the spatial distribution patterns of the HMs; (iii) HM accumulation were inversely proportional to soil pH, and were proportional to clay content, precipitation, and temperature; (iv) As, Cd, Hg, Pb, and Ni should be selected as the HMs to be controlled preferentially; (v) priority attention should be given to mining areas in Central South China, Southwest China, Liaoning province, and Zhejiang province; (vi) special attention should be given to mining areas of antimony, tin, tungsten, molybdenum, manganese, and lead‑zinc. The above results provided crucial information for HM control in the areas affected by mining at the national scale.
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http://dx.doi.org/10.1016/j.scitotenv.2022.154004DOI Listing
June 2022

SEEM: A Sequence Entropy Energy-Based Model for Pedestrian Trajectory All-Then-One Prediction.

IEEE Trans Pattern Anal Mach Intell 2022 Feb 1;PP. Epub 2022 Feb 1.

Predicting the future trajectories of pedestrians is of increasing importance for many applications such as autonomous driving and social robots. Nevertheless, current trajectory prediction models suffer from limitations such as lack of diversity in candidate trajectories, poor accuracy, and instability. In this paper, we propose a novel Sequence Entropy Energy-based Model named SEEM, which consists of a generator network and an energy network. Within SEEM we optimize the sequence entropy by taking advantage of the local variational inference of f-divergence estimation to maximize the mutual information across the generator in order to cover all modes of the trajectory distribution, thereby ensuring SEEM achieves full diversity in candidate trajectory generation. Then, we introduce a probability distribution clipping mechanism to draw samples towards regions of high probability in the trajectory latent space, while our energy network determines which trajectory is most representative of the ground truth. This dual approach is our so-called all-then-one strategy. Finally, a zero-centered potential energy regularization is proposed to ensure stability and convergence of the training process. Through experiments on both synthetic and public benchmark datasets, SEEM is shown to substantially outperform the current state-of-the-art approaches in terms of diversity, accuracy and stability of pedestrian trajectory prediction.
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http://dx.doi.org/10.1109/TPAMI.2022.3147639DOI Listing
February 2022

Effects of mining on the potentially toxic elements in the surrounding soils in China: A meta-analysis.

Sci Total Environ 2022 May 29;821:153562. Epub 2022 Jan 29.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, East Beijing Road 71, Nanjing 210008, China; University of Chinese Academy of Sciences, Yuquan Road 19, Beijing 100049, China.

Previous studies about the effects of mining on the potentially toxic elements (PTEs) in the surrounding soils mainly focused on single or few mining areas. However, these studies couldn't comprehensively quantify the mining-induced variations of soil PTE concentrations at the national scale. Moreover, the quantitative relationships between the effects of mining on soil PTEs and some related factors remained unclear at the national scale. This study first conducted a literature survey for soil PTE data affected by mining in China. Then, the random-effects model in the meta-analysis was used to quantify mining-induced variations of soil PTE concentrations in the surrounding areas. Last, the single meta-regression was used to explore the relationships between the effects of mining on soil PTEs and the related factors at the national scale. Results showed that: (i) mining-induced increases of soil PTE concentrations followed the order: Cd (1017%) > Hg (319%) > Pb (291%) > Zn (176%) > Cu (129%) > As (92%) > Ni (23%); (ii) mining-induced increases of soil PTE concentrations in clay (531%), non-ferrous mine (188%), paddy field (212%), and Central South China (290%) were more than those in other soil textures, mine types, land-use types, and geographical divisions, respectively; (iii) the effects of mining on soil PTEs were negatively correlated with soil pH (Q = 29.76, p < 0.01) and positively correlated with soil organic carbon (Q = 28.54, p < 0.01) and mean annual precipitation (Q = 91.75, p < 0.01); (iv) the effects of mining overall decreased with the sampling year (Q = 35.01, p < 0.01) and showed latitudinal zonality (Q = 180.39, p < 0.01). The above results provided valuable information for soil PTE mitigation in the areas affected by mining in China.
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http://dx.doi.org/10.1016/j.scitotenv.2022.153562DOI Listing
May 2022

Single-Cell Transcriptome Profiling Unravels Distinct Peripheral Blood Immune Cell Signatures of RRMS and MOG Antibody-Associated Disease.

Front Neurol 2021 14;12:807646. Epub 2022 Jan 14.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Relapsing-remitting multiple sclerosis (RRMS) and myelin oligodendrocyte glycoprotein (MOG) antibody-associated disease (MOGAD) are inflammatory demyelinating diseases of the central nervous system (CNS). Due to the shared clinical manifestations, detection of disease-specific serum antibody of the two diseases is currently considered as the gold standard for the diagnosis; however, the serum antibody levels are unpredictable during different stages of the two diseases. Herein, peripheral blood single-cell transcriptome was used to unveil distinct immune cell signatures of the two diseases, with the aim to provide predictive discrimination. Single-cell RNA sequencing (scRNA-seq) was conducted on the peripheral blood from three subjects, i.e., one patient with RRMS, one patient with MOGAD, and one patient with healthy control. The results showed that the CD19 CXCR4 naive B cell subsets were significantly expanded in both RRMS and MOGAD, which was verified by flow cytometry. More importantly, RRMS single-cell transcriptomic was characterized by increased naive CD8 T cells and cytotoxic memory-like Natural Killer (NK) cells, together with decreased inflammatory monocytes, whereas MOGAD exhibited increased inflammatory monocytes and cytotoxic CD8 effector T cells, coupled with decreased plasma cells and memory B cells. Collectively, our findings indicate that the two diseases exhibit distinct immune cell signatures, which allows for highly predictive discrimination of the two diseases and paves a novel avenue for diagnosis and therapy of neuroinflammatory diseases.
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http://dx.doi.org/10.3389/fneur.2021.807646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8795627PMC
January 2022

Genetic Characterization of -Positive Multidrug-Resistant Serotype Typhimurium Isolated From Intestinal Infection in Children and Pork Offal in China.

Front Microbiol 2021 10;12:774797. Epub 2022 Jan 10.

Center for Disease Control and Prevention of PLA, Beijing, China.

With the rapid emergence of plasmid-mediated colistin resistance gene , the increased resistance of has attracted extensive attention. This study reports on 11 multidrug-resistant serovar Typhimurium strains harboring in China. They all presented resistance to colistin, and additionally, one that was isolated from a child's stool sample was also resistant to ceftriaxone and azithromycin. We screened 1454 strains of for gene through PCR, and these strains are all preserved in our laboratory. Antimicrobial sensitivity analysis was carried out for the screened positive strains. Genetic polymorphism analysis of Typhimurium was performed by using the Pulsed-Field Gel Electrophoresis (PFGE). The plasmids harboring were identified by S1-PFGE and southern blotting. Plasmid conjugation assays were used to analyze the transferability of colistin resistance. The plasmids harboring were characterized by sequencing and bioinformatic analysis. Eleven Typhimurium strains harboring with colistin resistance (MICs 4μg/ml) were detected, which were isolated from children and pig offal in China. All of them were multidrug-resistant strains. PFGE results revealed that the strains isolated from different samples or locations have identical genotypes. S1-PFGE and southern blotting experiments showed that three plasmids of different sizes (33, 60, and 250 kb) all carried the gene. The plasmid conjugation assays revealed that acquired harboring plasmids by horizontal transfer. Sequencing and plasmid type analysis revealed that these plasmids were types IncX4, IncI2, and IncHI2. Among them, IncX4 and IncI2 plasmids had extremely similar backbones and contained one resistant gene . IncHI2 plasmid contained multiple resistant genes including , , , , , and . We identified 11 harboring Typhimurium strains in China and described their characteristics. Our findings indicate that the gene can effectively spread among intestinal bacteria by horizontal transfer of three types of plasmids. Moreover, the IncHI2 plasmid can also mediate the transfer of other drug resistance genes. These results reveal that constant surveillance of harboring Typhimurium is imperative to prevent the spread of colistin resistance.
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http://dx.doi.org/10.3389/fmicb.2021.774797DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784875PMC
January 2022

miR395-regulated sulfate metabolism exploits pathogen sensitivity to sulfate to boost immunity in rice.

Mol Plant 2022 04 28;15(4):671-688. Epub 2021 Dec 28.

National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

MicroRNAs (miRNAs) play important roles in plant physiological activities. However, their roles and molecular mechanisms in boosting plant immunity, especially through the modulation of macronutrient metabolism in response to pathogens, are largely unknown. Here, we report that an evolutionarily conserved miRNA, miR395, promotes resistance to Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc), two destructive bacterial pathogens, by regulating sulfate accumulation and distribution in rice. Specifically, miR395 targets and suppresses the expression of the ATP sulfurylase gene OsAPS1, which functions in sulfate assimilation, and two sulfate transporter genes, OsSULTR2;1 and OsSULTR2;2, which function in sulfate translocation, to promote sulfate accumulation, resulting in broad-spectrum resistance to bacterial pathogens in miR395-overexpressing plants. Genetic analysis revealed that miR395-triggered resistance is involved in both pathogen-associated molecular pattern-triggered immunity and R gene-mediated resistance. Moreover, we found that accumulated sulfate but not S-metabolites inhibits proliferation of pathogenic bacteria, revealing a sulfate-mediated antibacterial defense mechanism that differs from sulfur-induced resistance. Furthermore, compared with other bacteria, Xoo and Xoc, which lack the sulfate transporter CysZ, are sensitive to high levels of extracellular sulfate. Accordingly, miR395-regulated sulfate accumulation impaired the virulence of Xoo and Xoc by decreasing extracellular polysaccharide production and biofilm formation. Taken together, these results suggest that rice miR395 modulates sulfate metabolism to exploit pathogen sensitivity to sulfate and thereby promotes broad-spectrum resistance.
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http://dx.doi.org/10.1016/j.molp.2021.12.013DOI Listing
April 2022

Sandwich-Structured [email protected] Hybrid Anodes with High Initial Coulombic Efficiency for High-Rate Lithium-Ion Batteries.

ACS Appl Mater Interfaces 2021 Dec 15;13(51):61055-61066. Epub 2021 Dec 15.

College of Material Science and Engineering, Hunan University, Changsha, Hunan 410082, P. R. China.

The high theoretical capacity makes metal phosphides appropriate anode candidates for Li-ion batteries, but their applications are restricted due to the limited structural instability caused by the huge volume change, as in other high-capacity materials. Here, we design an integrated electrode consisting of SnP nanoparticles sandwiched between transition-metal carbide (MXene) nanosheets. Tetramethylammonium hydroxide (TMAOH) plays an essential role in the formation of such sandwich structures by producing negatively charged MXene sheets with expanded layer spacings. The strong C-O-P oxygen bridge bond enables tight anchoring of SnP nanoparticles on the surface of MXene layers. The obtained SnP-based nanocomposites exhibit high reversible capacity with an initial Coulombic efficiency of 82% and outstanding rate performance (1519 mAh cm at a current density of 5 A g). The conductive and flexible MXene layers on both sides of SnP nanoparticles provide the desired electric conductivity and elastomeric space to accommodate the large volume change of SnP during lithiation. Therefore, the [email protected] hybrid exhibits an enhanced cyclic performance of 820 mAh g after 300 cycles at a current density of 1 A g.
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http://dx.doi.org/10.1021/acsami.1c17297DOI Listing
December 2021

Meta-analyses identify DNA methylation associated with kidney function and damage.

Nat Commun 2021 12 9;12(1):7174. Epub 2021 Dec 9.

Department of Chronic Diseases Epidemiology, National Center for Epidemiology, Carlos III Health Institute, Madrid, Spain.

Chronic kidney disease is a major public health burden. Elevated urinary albumin-to-creatinine ratio is a measure of kidney damage, and used to diagnose and stage chronic kidney disease. To extend the knowledge on regulatory mechanisms related to kidney function and disease, we conducted a blood-based epigenome-wide association study for estimated glomerular filtration rate (n = 33,605) and urinary albumin-to-creatinine ratio (n = 15,068) and detected 69 and seven CpG sites where DNA methylation was associated with the respective trait. The majority of these findings showed directionally consistent associations with the respective clinical outcomes chronic kidney disease and moderately increased albuminuria. Associations of DNA methylation with kidney function, such as CpGs at JAZF1, PELI1 and CHD2 were validated in kidney tissue. Methylation at PHRF1, LDB2, CSRNP1 and IRF5 indicated causal effects on kidney function. Enrichment analyses revealed pathways related to hemostasis and blood cell migration for estimated glomerular filtration rate, and immune cell activation and response for urinary albumin-to-creatinineratio-associated CpGs.
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http://dx.doi.org/10.1038/s41467-021-27234-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8660832PMC
December 2021
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