Publications by authors named "Hongbo Chen"

300 Publications

Health status and public health education for internal older migrants in China: Evidence from a nationally representative survey.

Front Public Health 2022 22;10:937361. Epub 2022 Jul 22.

Nursing School of Peking University Health Science Center, Beijing, China.

Background: Self-rated health has been widely used as a useful screening tool to subjectively evaluate individuals' health status. Under the context of the rapid growth of aging, there was a dramatic rapid expansion in internal older migrants in China. Serious concerns on the issues of health status continue to attract quite a lot of attention over the past decades. Public health education is one of the most important health care services and methods to improve individuals' health status. However, most previous studies focus on the utilization of public health services such as visiting to doctors, physical examination, and hospitalization. There was limited evidence on the self-rated health and public health education of older migrants.

Objectives: The study aimed to evaluate self-rated health and the associated determinants in older migrants, as well as to gain a deeper insight into the current status of public health education received by older migrants.

Methods: We derived the data from the National Migrants Population Health and Family Planning Dynamic Monitor Survey 2018, a cross-sectional study, for secondary analysis. Internal migrants aged 60 years old or over were included in the study. Self-rated health was the dependent variable, while sociodemographic characteristics were the independent variable. The univariate and multivariate analyses were performed by Stata 15.1.

Results: A total of 5,589 older migrants were included in the study. Eighty-two percentage of older migrants reported healthy self-rated health. There was a significant association between age, gender, minzu, education level, monthly income, public health education, and self-rated health ( < 0.5). However, the proportion of older migrants that received specific public health education was <50%. The most common approach to receiving public health education was through the leaflet, while <20% of older migrants received public health education through public consultation and one-to-one education.

Conclusions: It was necessary to promote the publicity of public health education in older migrants through easy access and diverse approaches in order to effectively spread health-related knowledge to older migrants to satisfy their health needs and maintain their health.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2022.937361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353578PMC
August 2022

Effect of the Addition of Different Amounts of Aramid Fibers on Metal Friction and Wear during Mixing.

Polymers (Basel) 2022 Jul 21;14(14). Epub 2022 Jul 21.

National Engineering Laboratory for Advanced Tire Equipment and Key Materials, Qingdao 266061, China.

Studies show that the long-term operation of a rubber mixer results in wear at the end face of the mixer. End face wear increases the gap between the mixing chamber and the end face, resulting in leakage and a reduction in the mixing performance, affecting the final product's quality. Therefore, it is essential to investigate the wear of the metal face during the mixing process. The present study added aramid fibers to a rubber compound using a mechanical blender to obtain a composite material. Then, the influence of the aramid fibers on the metal friction and wear of the end face of the mixer was analyzed. This article introduces the concept of the wear ratio and explores the friction and wear of metals from the perspective of formulation technology for the first time. With the addition of aramid fibers, the proportion of abrasive wear of rubber on metal decreased, and the proportion of corrosive wear increased during the mixing process; however, when the addition of aramid fibers exceeded 3 phr, the balance of abrasive wear of rubber on metal increased and the proportion of corrosive wear decreased. It was found that aramid fibers have the property of friction reduction, which reduces the wear of the rubber blend on the metal. When the amount of aramid fibers added was 3 phr, the amount of abrasion of the rubber compound on the metal was the lowest.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym14142961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318473PMC
July 2022

Nanotech Probes: A Revolution in Cancer Diagnosis.

Front Oncol 2022 7;12:933125. Epub 2022 Jul 7.

Department of Plastic and Cosmetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Recent advances in nanotechnologies for cancer diagnosis and treatment have received considerable attention worldwide. Nanoparticles are being used to create nanodrugs and probes to diagnose and treat a variety of diseases, including cancer. Nanomedicines have unique advantages, such as increased surface-to-volume ratios, which enable them to interact with, absorb, and deliver small biomolecules to a very specific target, thereby improving the effectiveness of both probes and drugs. Nanoprobe biotechnology also plays an important role in the discovery of novel cancer biomarkers, and nanoprobes have become an important part of early clinical diagnosis of cancer. Various organic and inorganic nanomaterials have been developed as biomolecular carriers for the detection of disease biomarkers. Thus, we designed this review to evaluate the advances in nanoprobe technology in tumor diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.933125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300983PMC
July 2022

Synchronous Signal Transmission Method of Minimally Invasive Renal Failure Surgery Based on Nano Molecular Image Probe.

J Biomed Nanotechnol 2022 Apr;18(4):1205-1214

Department of Urology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefectrue, Enshi, Hubei, 445000, China.

The synchronous signal transmission can promote the successful completion of minimally invasive renal failure surgery, so the synchronous signal transmission method of minimally invasive renal failure surgery based on nano molecular image probe is studied. The nanoprobe is constructed by loading different signal molecules, photosensitizers, acoustic sensitizers and thermosensitives through the method of double emulsion or film hydration; the near-infrared confocal endoscope molecular image diagnosis and treatment equipment is designed based on the nanoprobe, and the intraoperative highfrequency image is obtained through the diagnosis and treatment equipment; the high-frequency injection energy and signal synchronous transmission method is adopted, and the signal is added to the primary and secondary resonance circuit. In the coupling module, a higher frequency alternating signal is added into the resonant coupling voltage to modulate the signal into the transmission resonance system, which is transmitted from the energy transmission coupling coil to the secondary end, and the injected signal part of the coupling coil is demodulated at the other end to complete the transmission of the signal coupling transmission loop, so as to realize the high-frequency image energy and index data signal of minimally invasive renal failure surgery. The experimental results show that the designed nano molecular image probe has good stability, and the simulated signal transmission waveform is consistent with the transmission waveform of the principle analysis. Applying the proposed method to the minimally invasive surgery of renal failure, it is found that the success rate and image signal transmission efficiency of the minimally invasive surgery of patients 1-5 are higher than 99.5%, and the operation image transmission accuracy is high and the operation effect is excellent.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1166/jbn.2022.3326DOI Listing
April 2022

PHF6 functions as a tumor suppressor by recruiting methyltransferase SUV39H1 to nucleolar region and offers a novel therapeutic target for PHF6-muntant leukemia.

Acta Pharm Sin B 2022 Apr 30;12(4):1913-1927. Epub 2021 Oct 30.

School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, China.

Mutations in the plant homeodomain-like finger protein 6 () gene are strongly associated with acute myeloid (AML) and T-cell acute lymphoblastic leukemia (T-ALL). In this study, we demonstrated that PHF6 can bind to H3K9me3 and H3K27me1 on the nucleolar chromatin and recruit histone methyltransferase SUV39H1 to the rDNA locus. The deletion of caused a decrease in the recruitment of SUV39H1 to rDNA gene loci, resulting in a reduction in the level of H3K9me3 and the promotion of rDNA transcription. The knockdown of either or significantly attenuated the effects of increase in H3K9me3 and suppressed the transcription of rDNA induced by the overexpression of the other interacting partner, thereby establishing an interdependent relationship between PHF6 and SUV39H1 in their control of rRNA transcription. The clinical mutants significantly impaired the ability to bind and recruit SUV39H1 to the rDNA loci, resulting in an increase in rDNA transcription activity, the proliferation of leukemia cells, and the growth of mouse xenografts. Importantly, significantly elevated levels of pre-rRNA were observed in clinical AML patients who possessed a mutated version of . The specific rDNA transcription inhibitor CX5461 significantly reduced the resistance of U937 AML cells deficient in to cytarabine, the drug that is most commonly used to treat AML. Collectively, we revealed a novel molecular mechanism by which PHF6 recruits methyltransferase SUV39H1 to the nucleolar region in leukemia and provided a potential therapeutic target for -mutant leukemia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.apsb.2021.10.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279718PMC
April 2022

Determinants of depressive symptom trajectories in self-reported chronic obstructive pulmonary disease patients.

BMC Pulm Med 2022 Jul 17;22(1):274. Epub 2022 Jul 17.

Peking University School of Nursing, 38 Xueyuan Road, Haidian District, Beijing, China.

Background: The depressive symptom trajectories of COPD individuals and its' predictors remain to be established. Therefore, this study aimed to explore the trajectories of depressive symptoms and predictors thereof in COPD patients.

Methods: A total of 1286 individuals over 45 years of age with self-reported COPD were assessed. Depressive symptoms were evaluated using the Center for Epidemiological Studies Depression Scale short form, with depressive symptom trajectories being identified via latent class growth analysis. The predictors of depressive symptom trajectories were then identified through multinomial logistic regression.

Results: Finally, three depressive symptom trajectories were identified: "steadily high", "consistently moderate", and "consistently low". Old age, longer night-time sleep duration, and high BMI were found to be associated with individuals being classified under the "consistently moderate" trajectory. Moreover, participants exhibiting more than two chronic conditions were more likely to be classified under the "consistently moderate" trajectory. Higher education and lower hand grip strength were important predictors of individuals classified in the "steadily high" trajectory.

Conclusions: To conclude, three depressive symptom trajectories were identified in self-reported COPD individuals. To ensure timely intervention aimed at preventing the worsening of depressive symptom progression among COPD individuals, health-care workers should regular analyze depressive symptoms and provide appropriate interventions when possible.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12890-022-02060-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288686PMC
July 2022

Nanoparticle-Based Combination Therapy for Melanoma.

Front Oncol 2022 28;12:928797. Epub 2022 Jun 28.

Department of Plastic and Cosmetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Melanoma is a cutaneous carcinoma, and its incidence is rapidly increasing with every year. The treatment options for melanoma have been comprehensively studied. Conventional treatment methods (e.g., radiotherapy, chemotherapy and photodynamic therapy) with surgical removal inevitably cause serious complications; moreover, resistance is common. Nanoparticles (NPs) combined with conventional methods are new and promising options to treat melanoma, and many combinations have been achieving good success. Due to their physical and biological features, NPs can help target intended melanoma cells more efficiently with less damage. This creates new hope for a better treatment strategy for melanoma with minimum damage and maximum efficacy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.928797DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273962PMC
June 2022

Research Progress in Exosome-Based Nanoscale Drug Carriers in Tumor Therapies.

Front Oncol 2022 21;12:919279. Epub 2022 Jun 21.

Department of Burn and Plastic Surgery, Affiliated Hospital 2 of Nantong University, Nantong First People's Hospital, Nantong, China.

Current antitumor treatment methods have several reported limitations, including multidrug resistance and serious adverse reactions. Targeted drug delivery systems are effective alternatives that can help healthcare providers overcome these limitations. Exosomes can serve as a natural nanoscale drug delivery system, with the advantages of high biocompatibility, low immunogenicity, and efficient tumor targetability. In this paper, we discuss the biological characteristics of exosomes, summarize the drug-carrying mechanisms of exosome-based drug delivery systems, and examine the potential role and applicability of exosomes in clinical tumor treatment approaches. This review can be used as a guideline for the future development of exosome-based delivery systems in clinical precision tumor treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.919279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253528PMC
June 2022

Case Report: Fanconi-Bickel Syndrome in a Chinese Girl With Diabetes and Severe Hypokalemia.

Front Pediatr 2022 9;10:897636. Epub 2022 Jun 9.

Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, China.

Fanconi-Bickel syndrome (FBS) is a rare autosomal recessive carbohydrate metabolism disorder. The main symptoms of FBS are hepatomegaly, nephropathy, postprandial hyperglycemia, fasting hypoglycemia, and growth retardation. Hypokalemia is a rare clinical feature in patients with FBS. In this study, we present a neonate suffering from FBS. She presented with hypokalemia, dysglycaemia, glycosuria, hepatomegaly, abnormality of liver function, and brain MRI. Trio whole-exome sequencing (WES) and Sanger sequencing were performed to identify the causal gene variants. A compound heterozygous mutation (NM_000340.2; p. Trp420*) of was identified. Here, we report a patient with FBS in a consanguineous family with diabetes, severe hypokalemia, and other typical FBS symptoms. Patients with common clinical features may be difficult to diagnose just by phenotypes in the early stage of life, but WES could be an important tool. We also discuss the use of insulin in patients with FBS and highlight the importance of a continuous glucose monitoring system (CGMS), not only in diagnosis but also to avoid hypoglycemic events.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fped.2022.897636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218529PMC
June 2022

Retard or exacerbate: Role of long non-coding RNA growth arrest-specific 5 in the fibrosis.

Cytokine Growth Factor Rev 2022 Jun 17. Epub 2022 Jun 17.

The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Hangzhou 310006, China. Electronic address:

Fibrosis is the endpoint of pathological remodeling involving different expressions of non-coding RNA(ncRNA) including long non-coding RNA growth arrest-specific 5 (lncRNA Gas5). Up to now, many studies have demonstrated that lncRNA Gas5 may play a vital regulatory role in the occurrence and development of organ fibrosis including liver, renal and cardiac fibrosis et al. Furthermore, Gas5 may also serve as a biomarker in diagnostic settings for fibrosis diseases. Structurally, IncRNA Gas5 impacts fibrosis via its distinct structural modules. In response to various external stresses, distinct functional complexes on different parts of Gas5 sequence influence cell proliferation and survival, thus affecting the inflammatory process and deposition of extracellular matrix(ECM) in organ fibrosis. However, there is no consensus on the role of Gas5 in fibrosis and its changed expression under various circumstances. In this review, we present an overview of what is known about the effect of Gas5 in organ fibrosis so far and for the first time explain its mechanism in the progression of fibrosis based on its unique structure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cytogfr.2022.06.001DOI Listing
June 2022

PD-1 Cellular Nanovesicles Carrying Gemcitabine to Inhibit the Proliferation of Triple Negative Breast Cancer Cell.

Pharmaceutics 2022 Jun 14;14(6). Epub 2022 Jun 14.

School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China.

PD-1 inhibitor Keytruda combined with chemotherapy for Triple-negative breast cancer (TNBC) has been approved for FDA, successfully representing the combination therapy of immunotherapy and chemotherapy for the first time in 2020. However, PD-L1 inhibitor Tecentriq combined with albumin paclitaxel using the similar strategy failed to achieve the expected effect. Therefore, it is still necessary to explore new effective immunotherapy and chemotherapy-based combined strategies. We developed a cell membrane-derived programmed death-ligand 1(PD-1) nanovesicle to encapsulate low-dose gemcitabine (PD-1&GEM NVs) to study the effect on breast cancer in vitro and in vivo. We found that engineered PD-1&GEM NVs could synergistically inhibit the proliferation of triple-negative breast cancer, which interacted with PD-L1 in triple-negative breast cancer to disrupt the PD-L1/PD-1 immune inhibitory axis and promoted cancer cell apoptosis. Moreover, PD-1&GEM NVs had better tumor targeting ability for PD-L1 highly-expressed TNBC cells, contributing to increasing the drug effectiveness and reducing toxicity. Importantly, gemcitabine-encapsulated PD-1 NVs exerted stronger effects on promoting apoptosis of tumor cells, increasing infiltrated CD8 T cell activation, delaying the tumor growth and prolonging the survival of tumor-bearing mice than PD-1 NVs or gemcitabine alone. Thus, our study highlighted the power of combined low-dose gemcitabine and PD-1 in the nanovesicles as treatment to treat triple-negative breast cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/pharmaceutics14061263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229990PMC
June 2022

Smart Nanoparticles for Breast Cancer Treatment Based on the Tumor Microenvironment.

Front Oncol 2022 26;12:907684. Epub 2022 May 26.

Department of Plastic and Cosmetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Breast cancer (BC) is the most common malignant tumor in women. There are different risk characteristics and treatment strategies for different subtypes of BC. The tumor microenvironment (TME) is of great significance for understanding the occurrence, development, and metastasis of tumors. The TME plays an important role in all stages of BC metastasis, immune monitoring, immune response avoidance, and drug resistance, and also plays an important role in the diagnosis, prevention, and prognosis of BC. Smart nanosystems have broad development prospect in the regulation of the BC drug delivery based on the response of the TME. In particular, TME-responsive nanoparticles cleverly utilize the abnormal features of BC tissues and cells to achieve targeted transport, stable release, and improved efficacy. We here present a review of the mechanisms underlying the response of the TME to BC to provide potential nanostrategies for future BC treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.907684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204624PMC
May 2022

Effect and Safety of Adding Metformin to Insulin Therapy in Treating Adolescents With Type 1 Diabetes Mellitus: An Updated Meta-Analysis of 10 Randomized Controlled Trials.

Front Endocrinol (Lausanne) 2022 30;13:878585. Epub 2022 May 30.

Center of Translational Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.

Background: The role of metformin in the treatment of adolescents with type 1 diabetes mellitus (T1DM) remains controversial. We conducted this updated meta-analysis to generate a comprehensive assessment regarding the effect and safety of metformin in treating adolescents with T1DM.

Methods: We systematically searched PubMed, Embase, and the Cochrane Central Registry of Controlled Trials (CENTRAL) from their inception to November 2021 to identify randomized controlled trials evaluating the efficacy of metformin in the treatment of adolescents with T1DM. The primary outcome was the HbA1c level, and secondary outcomes included the body mass index (BMI), total insulin daily dose (TIDD) (unit/kg/d), hypoglycemia events, diabetes ketoacidosis (DKA) events, and gastrointestinal adverse events (GIAEs). Statistical analysis was conducted using RevMan 5.4 and STATA 14.0.

Results: Ten studies enrolling 539 T1DM adolescents were included. Results suggested that metformin significantly decreased the HbA1c level at 12 months (mean difference [MD])=-0.50, 95% confidence interval [CI]=-0.61 to -0.39, P < 0.01); BMI (kg/m) at 3 months (MD=-1.05, 95%CI=-2.05 to -0.05, P=0.04); BMI z-score at 6 months (MD=-0.10, 95%CI=-0.14 to -0.06, P<0.01); and TIDD at 3 (MD=-0.13, 95%CI=-0.20 to -0.06, P<0.01), 6 (MD=-0.18, 95%CI=-0.25 to -0.11, P<0.01), and 12 (MD=-0.42, 95%CI=-0.49 to -0.35, P<0.01) months but significantly increased the risk of hypoglycemia events (risk ratio [RR]=3.13, 95%CI=1.05 to 9.32, P=0.04) and GIAEs (RR=1.64, 95%CI=1.28 to 2.10, P<0.01). For remaining outcomes at other time points, no statistical difference was identified. Sensitivity analysis confirmed the robustness of all pooled results.

Conclusions: The use of metformin might result in decreased BMI (kg/m), BMI z-score, and TIDD and increased risk of hypoglycemia events and GIAEs in adolescents with T1DM. However, future studies are required to further confirm the optimal dose and duration of metformin therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2022.878585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9190285PMC
June 2022

Measurement properties of performance-based measures to assess physical function in knee osteoarthritis: A systematic review.

Clin Rehabil 2022 Jun 14:2692155221107731. Epub 2022 Jun 14.

School of Nursing, 12465Peking University, China, Beijing, China.

Objective: To systematically review the measurement properties of performance-based measures to assess physical function in people with knee osteoarthritis.

Data Sources: PubMed, Web of Science, Embase, Scopus, CINAHL, and PsycINFO were searched in May 2022.

Methods: This study was conducted in accordance with the guidelines recommended by the Preferred Reporting Items for Systematic Reviews and Meta-Analysis. Clinical trials on the psychometric properties of performance-based tools for measuring physical function in people with knee osteoarthritis were included. Two reviewers independently rated measurement properties using the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN). "Best evidence synthesis" was made using COnsensus-based Standards for the selection of health Measurement INstruments outcomes and the quality of findings.

Results: Thirty-six out of 3425 publications were eligible for inclusion. Thirty-two performance-based measures were evaluated including 26 single-activity measures and 6 multi-activity measures. Measurement properties evaluated included internal consistency (2 measures), reliability (23 measures), measurement error (20 measures), hypotheses testing for construct validity (22 measures), and responsiveness (23 measures). On balance of the limited evidence, the walk 40 m fast-paced test and 6-minute walking test were the best rated walking tests. The 30-second chair stand test and timed up and go test were the best rated sit-to-stand tests. The Performance Tests Measures and Physical Activity Restrictions may be the suitable multi-activity measures for knee osteoarthritis.

Conclusions: Further good quality research investigating the measurement properties, and in particular, the measurement error of performance-based measures in patients with knee osteoarthritis is needed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/02692155221107731DOI Listing
June 2022

WSRD-Net: A Convolutional Neural Network-Based Arbitrary-Oriented Wheat Stripe Rust Detection Method.

Front Plant Sci 2022 24;13:876069. Epub 2022 May 24.

Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Science, Hefei, China.

Wheat stripe rusts are responsible for the major reduction in production and economic losses in the wheat industry. Thus, accurate detection of wheat stripe rust is critical to improving wheat quality and the agricultural economy. At present, the results of existing wheat stripe rust detection methods based on convolutional neural network (CNN) are not satisfactory due to the arbitrary orientation of wheat stripe rust, with a large aspect ratio. To address these problems, a WSRD-Net method based on CNN for detecting wheat stripe rust is developed in this study. The model is a refined single-stage rotation detector based on the RetinaNet, by adding the feature refinement module (FRM) into the rotation RetinaNet network to solve the problem of feature misalignment of wheat stripe rust with a large aspect ratio. Furthermore, we have built an oriented annotation dataset of in-field wheat stripe rust images, called the wheat stripe rust dataset 2021 (WSRD2021). The performance of WSRD-Net is compared to that of the state-of-the-art oriented object detection models, and results show that WSRD-Net can obtain 60.8% AP and 73.8% Recall on the wheat stripe rust dataset, higher than the other four oriented object detection models. Furthermore, through the comparison with horizontal object detection models, it is found that WSRD-Net outperforms horizontal object detection models on localization for corresponding disease areas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2022.876069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171371PMC
May 2022

HySec-Flow: Privacy-Preserving Genomic Computing with SGX-based Big-Data Analytics Framework.

IEEE Int Conf Cloud Comput 2021 Sep 13;2021:733-743. Epub 2021 Nov 13.

University of Virginia.

Trusted execution environments (TEE) such as Intel's Software Guard Extension (SGX) have been widely studied to boost security and privacy protection for the computation of sensitive data such as human genomics. However, a performance hurdle is often generated by SGX, especially from the small enclave memory. In this paper, we propose a new Hybrid Secured Flow framework (called "HySec-Flow") for large-scale genomic data analysis using SGX platforms. Here, the data-intensive computing tasks can be partitioned into independent subtasks to be deployed into distinct secured and non-secured containers, therefore allowing for parallel execution while alleviating the limited size of Page Cache (EPC) memory in each enclave. We illustrate our contributions using a workflow supporting indexing, alignment, dispatching, and merging the execution of SGX- enabled containers. We provide details regarding the architecture of the trusted and untrusted components and the underlying Scorn and Graphene support as generic shielding execution frameworks to port legacy code. We thoroughly evaluate the performance of our privacy-preserving reads mapping algorithm using real human genome sequencing data. The results demonstrate that the performance is enhanced by partitioning the time-consuming genomic computation into subtasks compared to the conventional execution of the data-intensive reads mapping algorithm in an enclave. The proposed HySec-Flow framework is made available as an open-source and adapted to the data-parallel computation of other large-scale genomic tasks requiring security and scalable computational resources.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/CLOUD53861.2021.00098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165173PMC
September 2021

Formononetin Attenuates Renal Tubular Injury and Mitochondrial Damage in Diabetic Nephropathy Partly via Regulating Sirt1/PGC-1α Pathway.

Front Pharmacol 2022 12;13:901234. Epub 2022 May 12.

Department of Nephrology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Mitochondrial abnormality is one of the main factors of tubular injury in diabetic nephropathy (DN). Formononetin (FMN), a novel isoflavonoid isolated from membranaceus, has diverse pharmacological activities. However, the beneficial effects of FMN on renal tubular impairment and mitochondrial dysfunction in DN have yet to be studied. In this study, we performed tests in Streptozotocin (STZ) -induced diabetic rats to explore the therapeutic effects of FMN on DN. We demonstrated that FMN could ameliorate albuminuria and renal histopathology. FMN attenuated renal tubular cells apoptosis, mitochondrial fragmentation and restored expression of mitochondrial dynamics-associated proteins, such as Drp1, Fis1 and Mfn2, as well as apoptosis-related proteins, such as Bax, Bcl-2 and cleaved-caspase-3. Moreover, FMN upregulated the protein expression of Sirt1 and PGC-1α in diabetic kidneys. studies further demonstrated that FMN could inhibit high glucose-induced apoptosis of HK-2 cells. FMN also reduced the production of mitochondrial superoxide and alleviated mitochondrial membrane potential (MMP) loss. Furthermore, FMN partially restored the protein expression of Drp1, Fis1 and Mfn2, Bax, Bcl-2, cleaved-caspase-3, Sirt1 and PGC-1α in HK-2 cells exposure to high glucose. In conclusion, FMN could attenuate renal tubular injury and mitochondrial damage in DN partly by regulating Sirt1/PGC-1α pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2022.901234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133725PMC
May 2022

An Attention-Guided Spatiotemporal Graph Convolutional Network for Sleep Stage Classification.

Life (Basel) 2022 Apr 21;12(5). Epub 2022 Apr 21.

School of Computer Science and Engineering, The University of Aizu, Tsuruga, Ikki-machi, Aizu-Wakamatsu City 965-8580, Fukushima, Japan.

Sleep staging has been widely used as an approach in sleep diagnoses at sleep clinics. Graph neural network (GNN)-based methods have been extensively applied for automatic sleep stage classifications with significant results. However, the existing GNN-based methods rely on a static adjacency matrix to capture the features of the different electroencephalogram (EEG) channels, which cannot grasp the information of each electrode. Meanwhile, these methods ignore the importance of spatiotemporal relations in classifying sleep stages. In this work, we propose a combination of a dynamic and static spatiotemporal graph convolutional network (ST-GCN) with inter-temporal attention blocks to overcome two shortcomings. The proposed method consists of a GCN with a CNN that takes into account the intra-frame dependency of each electrode in the brain region to extract spatial and temporal features separately. In addition, the attention block was used to capture the long-range dependencies between the different electrodes in the brain region, which helps the model to classify the dynamics of each sleep stage more accurately. In our experiments, we used the sleep-EDF and the subgroup III of the ISRUC-SLEEP dataset to compare with the most current methods. The results show that our method performs better in accuracy from 4.6% to 5.3%, in Kappa from 0.06 to 0.07, and in macro-F score from 4.9% to 5.7%. The proposed method has the potential to be an effective tool for improving sleep disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/life12050622DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144567PMC
April 2022

Factors influencing depression in community-dwelling elderly patients with osteoarthritis of the knee in China: a cross-sectional study.

BMC Geriatr 2022 05 25;22(1):453. Epub 2022 May 25.

School of Nursing, Peking University, 38 Xueyuan Road, Haidian District, Beijing, 100191, China.

Background: Knee osteoarthritis (OA) and depression are both major health issues influencing the quality of elderly life. The aim of the present study was to explore the prevalence of depression and the factors influencing depression in community-dwelling elderly patients with OA of the knee in China.

Methods: We conducted a cross-sectional descriptive study. The study included 214 participants aged 60 and older diagnosed with OA of the knee. The depression of the elderly was measured by using the Geriatric Depression Scale (GDS). Participants were asked to complete a demographic questionnaire, the GDS, the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), the society dimension of Arthritis Impact Measurement Scales 2 (AIMS2). In addition, the participants performed a timed up and go test (TUG) and the stair-climb test (SCT).

Results: The average age of the participants was 69.2 ± 7.63 years old, their body mass index (BMI) was 25.2 ± 3.85, and their disease duration was 5.9 ± 7.72 years. The mean total score of the GDS was 4.43 ± 2.89, and the GDS scores correlated positively with pain (r = 0.45, P < 0.001), stiffness (r = 0.40, P < 0.001), physical function (r = 0.52, P < 0.001),TUG (r = 0.35, P < 0.001), and SCT (r = 0.47, P < 0.001) and negatively with social support (r = - 0.35, P < 0.001).Analysis using multiple regression demonstrated that physical function, social support, and SCT explained 36.8% of the variance in depression.

Conclusions: Our findings suggested that physical function, social support, and lower extremity strength were predictors of depressive symptoms in community-dwelling elderly people with OA of the knee. Focusing on this elderly group with increasing functional exercise, positive social interaction and support, and lower limb muscle strength training should help in the prevention of depression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12877-022-03117-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134602PMC
May 2022

Polydimethyldiallylammonium chloride induces oxidative stress in anaerobic digestion of waste activated sludge.

Bioresour Technol 2022 Jul 14;356:127331. Epub 2022 May 14.

College of Environment and Resources, Xiangtan University, Xiangtan 411105, China. Electronic address:

The effects and key mechanisms of polydimethyldiallylammonium chloride on anaerobic digestion of waste activated sludge were investigated. Polydimethyldiallylammonium chloride at 38.1 g/kg total solids substantially reduced cumulative methane production from 138.2 ± 5.5 to 49.4 ± 5.0 L CH/kg volatile solids added, a reduction of 64.3 ± 0.2%. The quaternary ammonium groups on polydimethyldiallylammonium chloride agglomerated sludge flocs by neutralizing negatively charged amino groups in in extracellular polymeric substances, which hindered the release of organic matter. Quaternary ammonium groups induce oxidative stress by inducing the production of reactive oxygen species, thereby inhibiting the activity of anaerobic digestive enzymes. In addition, quaternary amine groups reduced the abundance of hydrolyzing bacteria, acidifying bacteria, and acetylotrophic methanogens. Oxidative stress could be an underappreciated mechanism that quaternary ammonium groups deteriorate anaerobic digestion, which could be transformative for understanding the potential risks of quaternary ammonium cationic flocculants in biological sludge treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2022.127331DOI Listing
July 2022

Red cell adenylate kinase deficiency in China: molecular study of 2 new mutations (413G > A, 223dupA).

BMC Med Genomics 2022 05 4;15(1):102. Epub 2022 May 4.

Department of Peadiatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, China.

Background: Adenylate kinase (AK) is a monomolecular enzyme widely found in a variety of organisms. It mainly catalyses the reversible transfer of adenosine nucleotide phosphate groups and plays an important role in maintaining energy metabolism. AK deficiency is a rare genetic disorder that is related to haemolytic anaemia. Chronic haemolytic anaemia associated with AK deficiency is a rare condition, and only 14 unrelated families have been reported thus far. Moreover, only 11 mutations have been identified in the AK1 gene, with only 3 cases of psychomotor impairment.

Case Presentation: The patient was a 3-year-old boy with severe haemolytic anaemia and psychomotor retardation. A molecular study of the patient's AK gene revealed 2 different mutations: a heterozygous missense mutation in exon 6 (c.413G > A) and a heterozygous frameshift mutation in exon 5 (c.223dupA). Molecular modelling analyses indicated that AK gene inactivation resulted in a lack of AK activity. The patient recovered after regular blood transfusion therapy.

Conclusions: AK1 deficiency was diagnosed on the basis of low enzymatic activity and the identification of a mutation in the AK1 gene located on chromosome 9q. Here, we report the first case of moderate red cell AK1 deficiency associated with chronic nonspherocytic haemolytic anaemia (CNSHA) in China. The genetic mutations were confirmed by Sanger sequencing. The variants were classified as pathogenic by bioinformatics tools, such as ACMG/AMP guidelines, Mutation Taster, SIFT, MACP, REVEL and PolyPhen2.2. Based on our evidence and previous literature reports, we speculate that the site of the AK1 gene c.413G > A (p.Arg138His) mutation may be a high-frequency mutation site and the other mutation (c.223dupA) might be related to the neuropathogenicity caused by AK1 deficiency. NGS should be a part of newborn to early childhood screening to diagnose rare and poorly diagnosed genetic diseases as early as possible.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12920-022-01248-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066714PMC
May 2022

Vimentin Suppresses Inflammation and Tumorigenesis in the Mouse Intestine.

Front Cell Dev Biol 2022 25;10:862237. Epub 2022 Mar 25.

Cell Biology, Biosciences, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland.

Vimentin has been implicated in wound healing, inflammation, and cancer, but its functional contribution to intestinal diseases is poorly understood. To study how vimentin is involved during tissue injury and repair of simple epithelium, we induced colonic epithelial cell damage in the vimentin null (Vim) mouse model. Vim mice challenged with dextran sodium sulfate (DSS) had worse colitis manifestations than wild-type (WT) mice. Vim colons also produced more reactive oxygen and nitrogen species, possibly contributing to the pathogenesis of gut inflammation and tumorigenesis than in WT mice. We subsequently describe that CD11b macrophages served as the mainly cellular source of reactive oxygen species (ROS) production vimentin-ROS-pSTAT3-interleukin-6 inflammatory pathways. Further, we demonstrated that Vim mice did not develop colitis-associated cancer model upon DSS treatment spontaneously but increased tumor numbers and size in the distal colon in the azoxymethane/DSS model comparing with WT mice. Thus, vimentin has a crucial role in protection from colitis induction and tumorigenesis of the colon.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2022.862237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993042PMC
March 2022

Small Extracellular Vesicles Derived From MSCs Have Immunomodulatory Effects to Enhance Delivery of ASO-210 for Psoriasis Treatment.

Front Cell Dev Biol 2022 10;10:842813. Epub 2022 Mar 10.

School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China.

Mesenchymal stem cells (MSCs) have been increasingly used for treating autoimmune diseases due to their immune modulation functions, but inefficient homing to the target tissue and safety issues limits their wide application. Recently, increasing studies demonstrate small extracellular vesicles (sEVs) as key mediators of MSCs to exert their immunomodulatory effects. In this study, we found that sEVs derived from human umbilical cord MSCs stimulated by IFN-γ (IFNγ-sEVs) inhibited proliferation and activation of peripheral blood mononuclear cells and T cells . Furthermore, we confirmed that IFNγ-sEVs reduced psoriasis symptoms including thickness, erythema, and scales of skin lesions; exhausted Th17 cells, increased Th2 cells; and reduced enrichment of inflammatory cytokines such as IL-17A, IFN-γ, IL-6, and TNF-α in both spleen and skin lesions . Importantly, IFNγ-sEVs significantly improved the delivery efficiency and stability of ASO-210, the antisense oligonucleotides of miR-210 block the immune imbalance and subsequent psoriasis development. Our results reveal MSC-sEVs as promising cell-free therapeutic agents and ideal delivery vehicles of antisense oligonucleotides for psoriasis treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2022.842813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960430PMC
March 2022

Melanoma and Nanotechnology-Based Treatment.

Front Oncol 2022 9;12:858185. Epub 2022 Mar 9.

Department of Plastic and Cosmetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Melanoma is a malignant tumor arising in melanocytes from the basal layer of the epidermis and is the fifth most commonly diagnosed cancer in the United States. Melanoma is aggressive and easily metastasizes, and the survival rate is low. Nanotechnology-based diagnosis and treatment of melanoma have attracted increasing attention. Importantly, nano drug delivery systems have the advantages of increasing drug solubility, enhancing drug stability, prolonging half-life, optimizing bioavailability, targeting tumors, and minimizing side effects; thus, these systems can facilitate tumor cytotoxicity to achieve effective treatment of melanoma. In this review, we discuss current nanosystems used in the diagnosis and treatment of melanoma, including lipid systems, inorganic nanoparticles, polymeric systems, and natural nanosystems. The excellent characteristics of novel and effective drug delivery systems provide a basis for the broad applications of these systems in the diagnosis and treatment of melanoma, particularly metastatic melanoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.858185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959641PMC
March 2022

How Does Epstein-Barr Virus Interact With Other Microbiomes in EBV-Driven Cancers?

Front Cell Infect Microbiol 2022 23;12:852066. Epub 2022 Feb 23.

Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The commensal microbiome refers to a large spectrum of microorganisms which mainly consists of viruses and bacteria, as well as some other components such as protozoa and fungi. Epstein-Barr virus (EBV) is considered as a common component of the human commensal microbiome due to its spread worldwide in about 95% of the adult population. As the first oncogenic virus recognized in human, numerous studies have reported the involvement of other components of the commensal microbiome in the increasing incidence of EBV-driven cancers. Additionally, recent advances have also defined the involvement of host-microbiota interactions in the regulation of the host immune system in EBV-driven cancers as well as other circumstances. The regulation of the host immune system by the commensal microbiome coinfects with EBV could be the implications for how we understand the persistence and reactivation of EBV, as well as the progression of EBV-associated cancers, since majority of the EBV persist as asymptomatic carrier. In this review, we attempt to summarize the possible mechanisms for EBV latency, reactivation, and EBV-driven tumorigenesis, as well as casting light on the role of other components of the microbiome in EBV infection and reactivation. Besides, whether novel microbiome targeting strategies could be applied for curing of EBV-driven cancer is discussed as well.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2022.852066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904896PMC
April 2022

Methylome and Transcriptome-Based Integration Analysis Identified Molecular Signatures Associated With Meningitis Induced by .

Front Immunol 2022 25;13:840399. Epub 2022 Feb 25.

Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China.

() can elicit serious inflammatory responses and cause meningitis in piglets. Previous epigenetic studies have indicated that alterations in host DNA methylation may modify the inflammatory response to bacterial infection. However, to date, genome-wide analysis of the DNA methylome during meningitis caused by infection is still lacking. In this study, we employed an unbiased approach using deep sequencing to profile the DNA methylome and transcriptome from infected porcine brain (cerebrum) and integrated the data to identify key differential methylation regions/sites involved in the regulation of the inflammatory response. Results showed that DNA methylation patterns and gene expression profiles from porcine brain were changed after infection. The majority of the altered DNA methylation regions were found in the intergenic regions and introns and not associated with CpG islands, with only a low percentage occurring at promoter or exon regions. Integrated analysis of the DNA methylome and transcriptome identified a number of inversely and positively correlated genes between DNA methylation and gene expression, following the criteria of |logFC| > 0.5, |diffMethy| > 0.1, and < 0.05. Differential expression and methylation of two significant genes, semaphoring 4D () and von Willebrand factor A domain containing 1 (), were validated by qRT-PCR and bisulfite sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses demonstrated that DNA methylation inversely correlated genes in infected porcine brains were mainly involved with cell adhesion molecules (CAMs), bacterial invasion of epithelial cells, RIG-1-like receptor signaling pathways, and hematopoietic cell lineage signaling pathways. In addition, a protein-protein interaction network of differentially methylated genes found potential candidate molecular interactions relevant to the pathology of infection. To the best of our knowledge, this is the first attempt to integrate the DNA methylome and transcriptome data from infected porcine brains. Our findings will help understanding the contribution of genome-wide DNA methylation to the pathogenesis of meningitis in pigs and developing epigenetic biomarkers and therapeutic targets for the treatment of induced meningitis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.840399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913945PMC
May 2022

Bidirectional association between knee osteoarthritis and depressive symptoms: evidence from a nationwide population-based cohort.

BMC Musculoskelet Disord 2022 Mar 5;23(1):213. Epub 2022 Mar 5.

Peking University School of Nursing, No.38 Xueyuan Road, Haidian District, Beijing, China.

Background: Both knee osteoarthritis (KOA) and depressive symptoms (DS) are major public health issues affecting the quality of life. This study aimed to examine the association between KOA and DS.

Methods: Data were gathered from the China Health and Retirement Longitudinal Study in 2011-2015 which surveyed middle-aged to elderly individuals and their spouses in 28 provinces in China. An adjusted Cox proportional hazards regression model was used to estimate hazard ratios (HRs).

Results: The analysis for baseline KOA and the subsequent risk of DS was based on 2582 participants without baseline DS. During the follow-up, KOA patients were more likely to have DS than non-KOA participants (adjusted HR = 1.38: 95% CI = 1.23 to 1.83). The analysis for baseline DS and the subsequent risk of KOA was based on 4293 participants without baseline KOA, those with DS were more likely to develop KOA than non-DS participants (adjusted HR = 1.51: 95% CI = 1.26 to 1.81). Subgroup analysis showed sex and age had no significant moderating effect on the KOA-DS association.

Conclusions: Our results provide evidence that the association between KOA and DS is bidirectional. Therefore, primary prevention and management of KOA and DS should consider this relationship.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12891-022-05137-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897877PMC
March 2022

Cellular membrane-based vesicles displaying a reconstructed B cell maturation antigen for multiple myeloma therapy by dual targeting APRIL and BAFF.

Acta Biomater 2022 04 24;143:406-417. Epub 2022 Feb 24.

School of pharmaceutical sciences (Shenzhen), Sun Yat-sen University, Shenzhen, 518107, China. Electronic address:

Excessive secretion of cytokines (such as APRIL and BAFF) in the bone marrow microenvironment (BMM) plays an essential role in the formation of relapsed or refractory multiple myeloma (MM). Blocking the binding of excessive cytokines to their receptors is becoming a promising approach for MM therapy. Here, we proposed a strategy of engineering cell membrane-based nanovesicles (NVs) to reconstruct B cell maturation antigen (BCMA), a receptor of APRIL and BAFF, to capture excess APRIL/BAFF in BMM as a bait protein. Our results showed that reconstructed BCMA expressed on the membrane of NVs (Re-BCMA-NVs) retained the ability of binding to soluble and surface-bound APRIL/BAFF in BMM. Consequently, Re-BCMA-NVs blocked the activation of the NF-κB pathway, downregulating the expression of anti-apoptosis genes and cell cycle-related genes, and hence inhibiting MM cell survival. Importantly, Re-BCMA-NVs showed a synergistic anti-MM effect when administrated together with bortezomib (BTZ) in vitro and in vivo. Our NVs targeting multiple cytokines in cancer microenvironment provides a solution to enhance sensitivity of MM cells to BTZ-based therapy. STATEMENT OF SIGNIFICANCE: Excessive APRIL and BAFF is reported to promote the survival of MM cell and facilitate the formation of resistance to bortezomib therapy. In this study, we bioengineered cell membrane derived reconstructed BCMA nanovesicles (Re-BCMA-NVs) to capture both soluble and cell-surface APRIL and BAFF. These NVs inhibited the activation of NF-κB pathway and thus inhibit the survival of MM cells in 2D, 3D and subcutaneous mouse tumor models. Importantly, Re-BCMA-NVs showed a synergistic anti-MM effect when administrated together with bortezomib in vitro and in vivo. Taken together, our NVs targeting multiple cytokines in cancer microenvironment provides a solution to enhance sensitivity of MM cells to bortezomib-based therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2022.02.028DOI Listing
April 2022

Balance Function in Patients With COPD: A Systematic Review of Measurement Properties.

Clin Nurs Res 2022 Jul 24;31(6):1000-1013. Epub 2022 Feb 24.

Peking University School of Nursing, Beijing, China.

To conduct an overview, evaluation, and synthesis of measurement properties of instruments assessing balance of COPD patients. Electronic searches were performed in Web of Science, Scopus, Embase, PubMed, CINAHL, PsycINFO, WanFang, and China National Knowledge Infrastructure databases up to the end of April 2021. Two reviewers independently evaluated the methodological quality using the Consensus-based Standards for the Selection of Health Status Measurement Instrument checklist, and rated the overall quality level of evidence was graded based upon a modified Grades of Recommendation, Assessment, Development, and Evaluation approach. Finally, 12 instruments were included. The Berg Balance Scale and the Timed Up and Go test were the most frequently used tools to evaluate balance. None of 12 instruments provide any information regarding cross-cultural validation or criterion validation. High-quality studies exploring measurement properties with a focus on the criterion validity and cross-cultural validity of balance measurements in COPD patients are warranted.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/10547738221078902DOI Listing
July 2022

Assessing Bone Quality of the Spine in Children with Scoliosis Using the Ultrasound Reflection Frequency Amplitude Index Method: A Preliminary Study.

Ultrasound Med Biol 2022 05 16;48(5):808-819. Epub 2022 Feb 16.

School of Information Science and Technology, ShanghaiTech University, Shanghai, China; Shanghai Engineering Research Center of Energy Efficient and Custom AI IC, ShanghaiTech University, Shanghai, China. Electronic address:

Osteopenia is considered a common phenomenon in patients who have scoliosis. Quantitative ultrasound has been used to assess skeletal status for decades, and recently ultrasound imaging using reflection signals from vertebrae were as well applied to measure spinal curvatures in children with scoliosis. The objectives of this study were to develop a new method that can robustly extract a parameter from ultrasound spinal data for estimating bone quality of scoliotic patients and to investigate the potential of the parameter in predicting curve progression. The frequency amplitude index (FAI) was calculated based on the spectrum of the original radiofrequency signals reflected from the tissue-vertebra interface. The correlation between FAI and reflection coefficient was validated using decalcified bovine bone samples in vitro, and the FAIs of scoliotic subjects were investigated in vivo with reference to body mass index, Cobb angles and curve progression status. The results revealed that the intra-rater measures were highly reliable between different trials (intra-class correlation coefficient = 0.997). The FAI value was strongly correlated with the reflection coefficient of bone tissue (R = 0.824), and the lower FAI indicated the higher risk of curve progression for the non-mild scoliosis cases. This preliminary study found that the FAI method can provide a feasible and robust approach to assessment of the bone quality of spine and may be a promising factor in monitoring curve progression of patients who have adolescent idiopathic scoliosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ultrasmedbio.2022.01.002DOI Listing
May 2022
-->