Publications by authors named "Hong-Mei Ren"

15 Publications

  • Page 1 of 1

Lysine demethylase LSD1 delivered via small extracellular vesicles promotes gastric cancer cell stemness.

EMBO Rep 2021 May 31:e50922. Epub 2021 May 31.

State Key Laboratory of Esophageal Cancer Prevention & Treatment, Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education of China, Collaborative Innovation Center of New Drug Research and Safety Evaluation, Henan Province, Key Laboratory of Henan Province for Drug Quality and Evaluation, Institute of Drug Discovery and Development, School of Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, China.

Several studies have examined the functions of nucleic acids in small extracellular vesicles (sEVs). However, much less is known about the protein cargos of sEVs and their functions in recipient cells. This study demonstrates the presence of lysine-specific demethylase 1 (LSD1), which is the first identified histone demethylase, in the culture medium of gastric cancer cells. We show that sEVs derived from gastric cancer cells and the plasma of patients with gastric cancer harbor LSD1. The shuttling of LSD1-containing sEVs from donor cells to recipient gastric cancer cells promotes cancer cell stemness by positively regulating the expression of Nanog, OCT4, SOX2, and CD44. Additionally, sEV-delivered LSD1 suppresses oxaliplatin response of recipient cells in vitro and in vivo, whereas LSD1-depleted sEVs do not. Taken together, we demonstrate that LSD1-loaded sEVs can promote stemness and chemoresistance to oxaliplatin. These findings suggest that the LSD1 content of sEV could serve as a biomarker to predict oxaliplatin response in gastric cancer patients.
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http://dx.doi.org/10.15252/embr.202050922DOI Listing
May 2021

Design, synthesis, and biological evaluation of novel dual inhibitors targeting lysine specific demethylase 1 (LSD1) and histone deacetylases (HDAC) for treatment of gastric cancer.

Eur J Med Chem 2021 Aug 25;220:113453. Epub 2021 Apr 25.

School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, China. Electronic address:

LSD1 and HDAC are physical and functional related to each other in various human cancers and simultaneous pharmacological inhibition of LSD1 and HDAC exerts synergistic anti-cancer effects. In this work, a series of novel LSD1/HDAC bifunctional inhibitors with a styrylpyridine skeleton were designed and synthesized based on our previously reported LSD1 inhibitors. The representative compounds 5d and 5m showed potent activity against LSD1 and HDAC at both molecular and cellular level and displayed high selectivity against MAO-A/B. Moreover, compounds 5d and 5m demonstrated potent antiproliferative activities against MGC-803 and HCT-116 cancer cell lines. Notably, compound 5m showed superior in vitro anticancer potency against a panel of gastric cancer cell lines than ORY-1001 and SP-2509 with IC values ranging from 0.23 to 1.56 μM. Compounds 5d and 5m significantly modulated the expression of Bcl-2, Bax, Vimentin, ZO-1 and E-cadherin, induced apoptosis, reduced colony formation and suppressed migration in MGC-803 cancer cells. In addition, preliminary absorption, distribution, metabolism, excretion (ADME) studies revealed that compounds 5d and 5m showed acceptable metabolic stability in human liver microsomes with minimal inhibition of cytochrome P450s (CYPs). Those results indicated that compound 5m could be a promising lead compound for further development as a therapeutic agent in gastric cancers via LSD1 and HDAC dual inhibition.
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http://dx.doi.org/10.1016/j.ejmech.2021.113453DOI Listing
August 2021

LSD1 deletion represses gastric cancer migration by upregulating a novel miR-142-5p target protein CD9.

Pharmacol Res 2020 09 3;159:104991. Epub 2020 Jun 3.

State Key Laboratory of Esophageal Cancer Prevention & Treatment, Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education of China, Collaborative Innovation Center of New Drug Research and Safety Evaluation, Henan Province, Key Laboratory of Henan Province for Drug Quality and Evaluation, Institute of Drug Discovery and Development, School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, Henan 450001, China. Electronic address:

LSD1 (histone lysine specific demethylase 1) takes part in the physiological process of cell differentiation, EMT (epithelial-mesenchymal transition) and immune response. In this study, we found LSD1 expression in metastatic gastric cancer tissues was significantly higher than that in normal tissues. Furthermore, LSD1 deletion was found to suppress gastric cancer migration by decreasing intracellular miR-142-5p, which further led to the upregulation of migration suppressor CD9, a newly identified target of miR-142-5p. While LSD1 was reported as a demethylase of H3K4me1/2, H3K9me1/2 and several non-histone proteins, this is a new evidence for LSD1 as a functional regulator of miRNA. On the other hand, our data suggested that promoting the secretion of miR-142-5p using small extracellular vesicles as vehicles is a new mechanism for LSD1 abrogation to down-regulate intracellular miR-142-5p. Taken together, this study uncovered a new mechanism for LSD1 that can contribute to gastric cancer migration by facilitating miR-142-5p to target CD9.
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http://dx.doi.org/10.1016/j.phrs.2020.104991DOI Listing
September 2020

MG53 protects against contrast-induced acute kidney injury by reducing cell membrane damage and apoptosis.

Acta Pharmacol Sin 2020 Nov 18;41(11):1457-1464. Epub 2020 May 18.

Department of Cardiology, Daping Hospital, Army Medical University, Chongqing, 400042, China.

Mitsugumin 53 (MG53) is a tripartite motif family protein that has been reported to attenuate injury via membrane repair in different organs. Contrast-induced acute kidney injury (CI-AKI) is a common complication caused by the administration of iodinated contrast media (CM). While the cytotoxicity induced by CM leading to tubular cell death may be initiated by cell membrane damage, we wondered whether MG53 alleviates CI-AKI. This study was designed to investigate the effect of MG53 on CI-AKI and the underlying mechanism. A rat model of CI-AKI was established, and CI-AKI induced the translocation of MG53 from serum to injury sites on the renal proximal tubular (RPT) epithelia, as illustrated by immunoblot analysis and immunohistochemical staining. Moreover, pretreatment of rats with recombinant human MG53 protein (rhMG53, 2 mg/mL) alleviated iopromide-induced injury in the kidney, which was determined by measuring serum creatinine, blood urea nitrogen and renal histological changes. In vitro studies demonstrated that exposure of RPT cells to iopromide (20, 40, and 80 mg/mL) caused cell membrane injury and cell death, which were attenuated by rhMG53 (10 and 50 μg/mL). Mechanistically, MG53 translocated to the injury site on RPT cells and bound to phosphatidylserine to protect RPT cells from iopromide-induced injury. In conclusion, MG53 protects against CI-AKI through cell membrane repair and reducing cell apoptosis; therefore, rhMG53 might be a potential effective means to treat or prevent CI-AKI.
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http://dx.doi.org/10.1038/s41401-020-0420-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656601PMC
November 2020

TSPO ligands prevent the proliferation of vascular smooth muscle cells and attenuate neointima formation through AMPK activation.

Acta Pharmacol Sin 2020 Jan 12;41(1):34-46. Epub 2019 Sep 12.

Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, 400042, China.

Abnormal growth of the intimal layer of blood vessels (neointima formation) contributes to the progression of atherosclerosis and in-stent restenosis. Recent evidence shows that the 18-kDa translocator protein (TSPO), a mitochondrial membrane protein, is involved in diverse cardiovascular diseases. In this study we investigated the role of endogenous TSPO in neointima formation after angioplasty in vitro and in vivo. We established a vascular injury model in vitro by using platelet-derived growth factor-BB (PDGF-BB) to stimulate rat thoracic aortic smooth muscle cells (A10 cells). We found that treatment with PDGF-BB (1-20 ng/mL) dose-dependently increased TSPO expression in A10 cells, which was blocked in the presence of PKC inhibitor or MAPK inhibitor. Overexpression of TSPO significantly promoted the proliferation and migration in A10 cells, whereas downregulation of TSPO expression by siRNA or treatment with TSPO ligands PK11195 or Ro5-4864 (10 nM) produced the opposite effects. Furthermore, we found that PK11195 (10-10 nM) dose-dependently activated AMPK in A10 cells. PK11195-induced inhibition on the proliferation and migration of PDGF-BB-treated A10 cells were abolished by compound C (an AMPK-specific inhibitor, 10 nM). In rats with balloon-injured carotid arteries, TSPO expression was markedly upregulated in the carotid arteries. Administration of PK11195 (3 mg/kg every 3 days, ip), starting from the initial balloon injury and lasting for 2 weeks, greatly attenuated carotid neointima formation by suppressing balloon injury-induced phenotype switching of VSMCs (increased α-SMA expression). These results suggest that TSPO is a vascular injury-response molecule that promotes VSMC proliferation and migration and is responsible for the neointima formation after vascular injury, which provides a novel therapeutic target for various cardiovascular diseases including atherosclerosis and restenosis.
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http://dx.doi.org/10.1038/s41401-019-0293-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471478PMC
January 2020

Lysine demethylase 5B (KDM5B): A potential anti-cancer drug target.

Eur J Med Chem 2019 Jan 17;161:131-140. Epub 2018 Oct 17.

Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Co-innovation Center of Henan Province for New Drug R & D and Preclinical Safety, School of Pharmaceutical Sciences, Institute of Drug Discovery and Development, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, Henan, 450001, China. Electronic address:

Lysine demethylase 5B (KDM5B) is a histone demethylase identified in 2007, which is responsible for erasing H3K4me2/3 activation marker. It participates in multiple repressive transcriptional complexes around target gene promoters and performs wide regulatory effects on chromatin structure. Until now, there is growing evidence for the oncogenic function of KDM5B. As the H3K4me2/3 residue represents the transcription initiation site of the active transcription gene, and demethylation of H3K4 is associated with transcriptional repression, making it a potential participant in inhibiting the expression of tumor suppressors. Therefore, KDM5B is considered as a promising drug target for cancer therapy, and many medicinal chemists are trying to design and synthesize potent and selective KDM5B inhibitors with the aid of high-throughput screening, structure based drug design, and structure activity relationship studies. This review focuses on the basic biochemical and physiological function of KDM5B and its involved mechanisms in cancers, a comprehensive overview of KDM5B inhibitors is also introduced.
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http://dx.doi.org/10.1016/j.ejmech.2018.10.040DOI Listing
January 2019

Recent advances in peptide nucleic acid for cancer bionanotechnology.

Acta Pharmacol Sin 2017 Jun 17;38(6):798-805. Epub 2017 Apr 17.

College of Public Health, Nantong University, Nantong 226019, China.

Peptide nucleic acid (PNA) is an oligomer, in which the phosphate backbone has been replaced by a pseudopeptide backbone that is meant to mimic DNA. Peptide nucleic acids are of the utmost importance in the biomedical field because of their ability to hybridize with neutral nucleic acids and their special chemical and biological properties. In recent years, PNAs have emerged in nanobiotechnology for cancer diagnosis and therapy due to their high affinity and sequence selectivity toward corresponding DNA and RNA. In this review, we summarize the recent progresses that have been made in cancer detection and therapy with PNA biotechnology. In addition, we emphasize nanoparticle PNA-based strategies for the efficient delivery of drugs in anticancer therapies.
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http://dx.doi.org/10.1038/aps.2017.33DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5520190PMC
June 2017

Senobtusin, a novel alkaloid with amidine moiety from Senecio obtusatus Wall. ex DC.

Nat Prod Res 2017 Oct 28;31(20):2450-2453. Epub 2017 Mar 28.

a College of Life Science and Technology , Beijing University of Chemical Technology , Beijing , China.

Phytochemical investigation of Senecio obtusatus Wall. ex DC led to the isolation of a novel alkaloid, senobtusin (1). Compound 1 possesses an atypical amidine moiety, and the structure was determined on the basis of extensive NMR analysis and HR-ESI-MS technique. This is the first report of this class of natural products obtained from family Asteraceae, and the systematic importance was also discussed.
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http://dx.doi.org/10.1080/14786419.2017.1309532DOI Listing
October 2017

Predictors and in-hospital outcomes of preoperative acute kidney injury in patients with type A acute aortic dissection.

J Geriatr Cardiol 2016 Aug;13(8):679-684

Emergency & Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Background: Acute kidney injury (AKI) is common after surgery for acute aortic dissection (AAD) and increases in-hospital and long-term mortality. However, few data exist on the clinical and prognostic relevance of early preoperative AKI in patients with type A AAD. We aimed to determine the incidence and predictors of preoperative AKI and the impact of AKI on in-hospital outcomes in patients with type A AAD.

Methods: From May 2009 to June 2014, we retrospectively enrolled 178 patients admitted to our hospital within 48 h from symptom onset and receiving open surgery for type A AAD. The patients were divided into no AKI and AKI groups and staged with AKI severity according to the KDIGO criteria before surgery.

Results: AKI occurred in 41 patients (23.0%). The incidence of in-hospital complications was significantly higher in patients with preoperative AKI compared to no AKI (41.5% 9.5%, < 0.001), including renal infarction (7.3% 0, = 0.012), and it increased with AKI severity ( < 0.001). Patients with AKI had higher in-hospital mortality compared with patients without AKI, although no significant difference was found (14.6% 5.1%, = 0.079). Multivariate analysis indicated that male gender, diastolic blood pressure on admission and bilateral renal artery involvement were independent predictors of preoperative AKI in patients with type A AAD.

Conclusions: Early AKI before surgery was common in patients with type A AAD, and was associated with increased in-hospital complications. Male gender, diastolic blood pressure on admission and bilateral renal artery involvement were major predictors for preoperative AKI.
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http://dx.doi.org/10.11909/j.issn.1671-5411.2016.08.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067429PMC
August 2016

Nucleation Kinetics in Mixed NaNO3/Glycerol Droplets Investigated with the FTIR-ATR Technique.

J Phys Chem B 2016 Mar 9;120(11):2913-20. Epub 2016 Mar 9.

The Institute of Chemical Physics, Key Laboratory of Cluster Science, School of Chemistry, Beijing Institute of Technology , Beijing 100081, People's Republic of China.

The in situ infrared spectra of sodium nitrate (NaNO3) and mixed NaNO3/glycerol droplets with organic to inorganic molar ratio (OIR) of 1:8, 1:4, 1:2, 1:1, and 2:1 on the ZnSe substrate were collected using the Fourier transform infrared attenuated total reflection (FTIR-ATR) technique in the RH linearly decreasing process. When the efflorescence process occurred in the RH decreasing process, the stochastric transformation from NaNO3 droplets to NaNO3 solid particles resulted in gradually increasing of a new band at 836 cm(-1) and contineously decreasing of an initial band at 829 cm(-1), which were assigned to the v2-NO3(-) mode in crystal phase state and in liquid state, respectively. There were excellent isobesic points between the two bands in the transformation processes, indicating the synchronization between the disappearence of NO3(-) in solutions and the production of NaNO3 crystal. The nucleation ratio, i.e., the amount of the droplets crystallized at a given RH upon the total amount droplets, was obtained by using the absorbance of ν2-NO3(-) band at 836 cm(-1), which was used to calculate the nucleation rates of NaNO3 either for heterogeneous or for homogeneous nucleation process. While the glycerol molecules delayed the efflorescence RHs (ERH) of NaNO3 in the mixed NaNO3/glycerol droplets (OIR = 2:1) to 15%, greatly lower than the ERH for pure NaNO3 droplets at 62.5%, they also greatly suppressed the heterogeneous nucleation rate with increase of the OIR ratio. Two different kinetic mechanisms were suggested in the mixed droplets with OIR = 1:8, 1:4, 1:2, and 1:1, i.e., homogeneous nucleation at higher supersaturation and heterogeneous nucleation at lower supersaturation. For the mixed droplets with 2:1 OIR, they fell into the homogeneous nucleation region completely.
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http://dx.doi.org/10.1021/acs.jpcb.5b12442DOI Listing
March 2016

Simiao pill ameliorates renal glomerular injury via increasing Sirt1 expression and suppressing NF-κB/NLRP3 inflammasome activation in high fructose-fed rats.

J Ethnopharmacol 2015 Aug 25;172:108-17. Epub 2015 Jun 25.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210023, People's Republic of China. Electronic address:

Ethnopharmacological Relevance: Simiao pill is one of the most frequently prescriptions in traditional Chinese medicine to treat hyperuricemia and gout. This study was to investigate the protective effects of Simiao pill on renal glomerular injury in a rat model of high fructose intake.

Materials And Methods: Sprague-Dawley male rats were given 10% fructose in drinking water and standard laboratory chow for 4 weeks to induce hyperuricemia and metabolic syndrome. Then fructose-fed animals were randomly divided into four groups receiving water, Simiao pill (78.87 and 157.74 mg/kg) and allopurinol (5mg/kg) daily for next 6 weeks, respectively. Serum levels of uric acid, creatinine, triglyceride, total cholesterol, low density lipoprotein, blood urea nitrogen, insulin, as well as urinary albumin were measured. Oral glucose tolerance test (OGTT) was carried out. Kidney pathological changes were detected using periodic-acid schiff-stained (PAS) staining and transmission electron microscopy (TEM) analysis. Glomerular protein levels of nephrin, podocin, CD2-associated protein (CD2AP), interleukin (IL)-1β, sirtuin 1 (Sirt1), nuclear factor kappaB (NF-κB) and pyrin domain containing 3 (NLRP3) inflammasome were measured by Western blot.

Results: Simiao pill effectively restored high fructose-induced hyperuricemia and metabolic syndrome in rats. Simiao pill significantly increased protein levels of nephrin, podocin and CD2AP in renal glomeruli, improved renal inflammatory cell infiltration into interstitium and glomerular injury in high fructose-fed rats with reduction of urine albumin levels. Furthermore, Simiao pill up-regulated Sirt1 protein levels and suppressed NF-κB/NLRP3 inflammasome activation to reduce IL-1β in renal glomeruli of high fructose-fed rats.

Conclusions: The renal protective effects of Simiao pill may be associated with up-regulation of Sirt1 expression and suppression of NF-κB/NLRP3 inflammasome activation to reduce renal glomerular injury in high fructose-fed rats with metabolic syndrome.
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http://dx.doi.org/10.1016/j.jep.2015.06.015DOI Listing
August 2015

Relationship between acute kidney injury before thoracic endovascular aneurysm repair and in-hospital outcomes in patients with type B acute aortic dissection.

J Geriatr Cardiol 2015 May;12(3):232-8

Emergency & Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, and Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing, China.

Objective: Acute kidney injury (AKI) frequently occurs after catheter-based interventional procedures and increases mortality. However, the implications of AKI before thoracic endovascular aneurysm repair (TEVAR) of type B acute aortic dissection (AAD) remain unclear. This study evaluated the incidence, predictors, and in-hospital outcomes of AKI before TEVAR in patients with type B AAD.

Methods: Between 2009 and 2013, 76 patients were retrospectively evaluated who received TEVAR for type B AAD within 36 h from symptom onset. The patients were classified into no-AKI vs. AKI groups, and the severity of AKI was further staged according to kidney disease: improving global outcomes criteria before TEVAR.

Results: The incidence of preoperative AKI was 36.8%. In-hospital complications was significantly higher in patients with preoperative AKI compared with no-AKI (50.0% vs. 4.2%, respectively; P < 0.001), including acute renal failure (21.4% vs. 0, respectively; P < 0.001), and they increased with severity of AKI (P < 0.001). The maximum levels of body temperature and white blood cell count were significantly related to maximum serum creatinine level before TEVAR. Multivariate analysis showed that systolic blood pressure on admission (OR: 1.023; 95% CI: 1.003-1.044; P = 0.0238) and bilateral renal artery involvement (OR: 19.076; 95% CI: 1.914-190.164; P = 0.0120) were strong predictors of preoperative AKI.

Conclusions: Preoperative AKI frequently occurred in patients with type B AAD, and correlated with higher in-hospital complications and enhanced inflammatory reaction. Systolic blood pressure on admission and bilateral renal artery involvement were major risk factors for AKI before TEVAR.
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http://dx.doi.org/10.11909/j.issn.1671-5411.2015.03.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460165PMC
May 2015

Retrograde coronary venous infusion provides targeted cell engraftment into infarcted myocardium.

Int J Cardiol 2014 Mar 8;172(2):e279-81. Epub 2014 Jan 8.

Emergency & Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, China; Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing, China.

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http://dx.doi.org/10.1016/j.ijcard.2013.12.202DOI Listing
March 2014

In vivo and ex vivo effects of propofol on myocardial performance in rats with obstructive jaundice.

BMC Gastroenterol 2011 Dec 28;11:144. Epub 2011 Dec 28.

Department of Anesthesia, Putuo Hospital, Shanghai, China.

Background: Responsiveness of the "jaundiced heart" to propofol is not completely understood. The purpose of this study was to evaluate the effect of propofol on myocardial performance in rats with obstructive jaundice.

Methods: Male Sprague-Dawley rats (n = 40) were randomly allocated into two groups, twenty underwent bile duct ligation (BDL), and 20 underwent a sham operation. Seven days after the surgery, propofol was administered in vivo and ex vivo (Langendorff preparations). Heart rate, left ventricular end-systolic pressure (LVESP) left ventricular end-diastolic pressure (LVEDP), and maximal rate for left ventricular pressure rise and decline (± dP/dtmax ) were measured to determine the influence of propofol on the cardiac function of rats.

Results: Impaired basal cardiac function was observed in the isolated BDL hearts, whereas in vivo indices of basal cardiac function (LVESP and ± dP/dt) in vivo were significantly higher in rats that underwent BDL compared with controls. With low or intermediate concentrations of propofol, these indices of cardiac function were within the normal physiologic range in both groups, and responsiveness to propofol was unaffected by BDL. When the highest concentration of propofol was administrated, a significant decline in cardiac function was observed in the BDL group.

Conclusions: In rats that underwent BDL, basal cardiac performance was better in vivo and worse ex vivo compared with controls. Low and intermediate concentrations of propofol did not appear to impair cardiac function in rats with obstructive jaundice.
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http://dx.doi.org/10.1186/1471-230X-11-144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276443PMC
December 2011

[Protection of liver against ischemia/reperfusion injury by Kupffer cell mediated emulsified isoflurane preconditioning: experiment with rats].

Zhonghua Yi Xue Za Zhi 2007 Sep;87(35):2468-71

Department of Anesthesiology, Eastern Hepatobiliary surgery Hospital, Second Military medical University, Shanghai, China.

Objective: To investigate the protective effect of pretreatment with Kupffer cell mediated emulsified isoflurane on ischemia/reperfusion injury in liver.

Methods: Thirty-two SD rats underwent partial obstruction of liver blood flow for 30 m and were randomly divided into 4 equal groups during the operation: lipid emulsion control group (Group C, pretreated with intravenous injection of lipid emulsion), inhibition of Kupffer cell and preconditioning with lipid emulsion control group (Group KC, intraperitoneally injected with gadolinium chloride, inhibitor of Kupffer cells, and then pretreated with intravenous injection of lipid emulsion), preconditioning with emulsified isoflurane group (Group IP, intravenously injected with emulsified isoflurane), and Inhibition of Kupffer cells and preconditioning with isoflurane group (Group IK, intraperitoneally injected with gadolinium chloride and then intravenously injected with emulsified isoflurane), Then liver perfusion was recovered for 2 h and the rats were killed. Blood samples were collected from the vena cava to detect the serum alanine transaminase (ALT) and aspartate transaminase (AST). Specimens of left liver tissue were collected to undergo light microscopy. The contents of malonyldialdehyde (MDA) and superoxide dismutase (SOD) in the liver homogenate were examined with relevant kits.

Results: Compared with Group C, the serum ALT and AST of Group IP were significantly lower (both P < 0.05). The MDA level of Group IP was significantly lower than that of Group C, and the SOD level of Group IP was significantly higher than that of Group C (both P < 0.05). However, there were no significant differences in the serum levels of ALT and AST and levels of MDA and SOD in lever homogenate among Group KC, IK, and C. The pathological changes in liver were remarkably milder in Group IP then in Group C, however, there were no significant differences in the pathological changes among Groups KC, IK, and C.

Conclusion: Preconditioning with emulsified isoflurane protects the liver from ischemia/reperfusion injury and this effect me be mediated by Kupffer cells.
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September 2007