Publications by authors named "Hong Zheng"

1,029 Publications

  • Page 1 of 1

How Can the η-Type Fullerene-Metal Bond Survive? A Systematic Survey of Reactions between Mono-EMFs and (M'L) Dimers.

Inorg Chem 2021 Jul 21. Epub 2021 Jul 21.

Institute of Molecular Science & Applied Chemistry, School of Chemistry, MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, Xi'an Jiaotong University, Xi'an 710049, China.

Recently, one η-coordinated complex of endohedral metallofullerene (EMF) [email protected](9)-C[Re(CO)] has been synthesized and characterized with a highly efficient radical-coupling methodology by performing a photochemical reaction between [email protected](9)-C and [Re(CO)] complexes. Theoretical investigations with the density functional theory reveal that this complex is stabilized by an ionic C-Re bond. The reactions of [email protected](9)-C (M = Sc, Y, La) with [Re(CO)] suggest that the reaction energies differ little because of similar single occupied molecular orbitals (SOMOs) of [email protected](9)-C. In the reactions of [email protected](9)-C with various transition-metal complexes [M'L] (M' = Mn, Tc, Re, Fe, Ru, Os, Co, Rh, Ir), the C-M' bonds with Mn, Tc, Re, Fe, Ru, and Os can stably exist, whereas those with Co, Rh, and Ir are unstable. Further analyses disclose that, in each element group, the stability of the C-M' bond is mainly determined by the bond energy of the M'-M' bond, which is related to the orbital of the M'L species. Moreover, the very-low-energy orbitals and large geometrical distortions of M'(CO) (M' = Co, Rh, Ir) lead to poor stabilities of the C-M' (M' = Co, Rh, Ir) bonds. As comparison, the reactions of [email protected](6)-C and [email protected] have been investigated. The [email protected](6)-C structure is more reactive toward the [M'L] complexes than [email protected](9)-C thanks to a lower SOMO of [email protected](6)-C than that of [email protected](9)-C, which derives from position change of the Y atom in (6)-C during the reactions. However, the formation of [[email protected](6)-C] suppresses the formation of several C-M' bonds. The reactivity of [email protected] is weak due to a high LUMO+1 of C, which leads to a high SOMO of [email protected] We believe that this theoretical study provides primary principles of radical-coupling reactions of EMFs and will be valuable for future research of organometallic complexes of fullerene.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01271DOI Listing
July 2021

Sequential multiple mediation of cognitive fusion and experiential avoidance in the relationship between rumination and social anxiety among Chinese adolescents.

Anxiety Stress Coping 2021 Jul 21:1-11. Epub 2021 Jul 21.

Shanghai Changning Mental Health Center, Shanghai, People's Republic of China.

Background And Objectives: The potential mechanism by which rumination influences social anxiety through cognitive fusion and experiential avoidance proposed by the Acceptance and Commitment Therapy model has not been well-documented. This study, therefore, aimed to examine the sequential multiple mediation of the two processes.

Design: A cross-sectional survey was conducted.

Method: A total of 233 Chinese adolescents (42.06% girls) completed a set of printed self-report questionnaires measuring rumination, cognitive fusion, experiential avoidance, and social anxiety. The SPSS macro PROCESS (model 6) was used to test a sequential mediating model. A 95% confidence interval (CI) was calculated with 5000 bootstrapping re-samples.

Results: Bootstrap analyses indicated that there were indirect effects of rumination on social anxiety mediated by cognitive fusion together with experiential avoidance ( = 0.098, BootSE = 0.032, CI = 0.045 to 0.170), or solely by experiential avoidance ( = 0.048, BootSE = 0.020, CI = 0.014 to 0.093). The mediation of cognitive fusion alone was not significant ( = 0.065, BootSE = 0.038, CI = -0.006 to 0.144).

Conclusions: The results indicated the sequential mediating role of cognitive fusion and experiential avoidance, and the relative prominence of the latter in the association between rumination and social anxiety.
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http://dx.doi.org/10.1080/10615806.2021.1955864DOI Listing
July 2021

Individuals with Autistic Traits Exhibit Heightened Alexithymia But Intact Interoceptive-Exteroceptive Sensory Integration.

J Autism Dev Disord 2021 Jul 20. Epub 2021 Jul 20.

Neuropsychology and Applied Cognitive Neuroscience Laboratory, CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, 100101, China.

Interoceptive accuracy has been widely measured using the Heartbeat Tracking Test (HTT). We devised the novel paradigm of Interoception-Exteroception Synchronicity Judgement (IESJ) task to assess participants' interoceptive accuracy, exteroceptive accuracy, and the balancing score which reflected the ability to allocate attentions between interoceptive and exteroceptive signals. This study administered the behavioural paradigms of the HTT and the IESJ as well as other self-report scales to 119 typically-developing youths. Individuals with lower autistic traits (n = 30) showed comparable interoceptive accuracy, exteroceptive accuracy, and balancing scores as their higher autistic traits counterparts (n = 33). Taken together, the high autistic traits subgroup exhibited higher levels of alexithymia but not empathy or interoception than the low autistic traits subgroup.
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http://dx.doi.org/10.1007/s10803-021-05199-yDOI Listing
July 2021

The Preoperative Diagnostic Value of MRI and Otoneural Tests in Acoustic Neuroma.

Front Oncol 2021 29;11:626485. Epub 2021 Jun 29.

Department of Otolaryngology-Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Objectives: To determine the preoperative diagnostic accuracy of MRI and otoneural tests (ONT) for acoustic neuroma (AN) in a cohort of unselected patients with pontocerebellar angle tumors. To find a convenient way to screening out the potential asymptomatic AN patient earlier.

Design: This diagnostic accuracy study was performed in a central hospital and included a consecutive sample of unilateral incipient pontocerebellar angle tumor patients referred for MRI and ONT before surgery. Different AN features of MRI and ONT were collected and concluded into preoperative diagnostic variables or variable combinations. Those of MRI and ONT are analyzed and compared with biopsy results by multivariable receiver operating characteristic (ROC) analysis. The early-stage group, the course of which is 1 year or less, was separately computed and compared.

Results: Eighty-three subjects were collected from June 2013 to June 2019; 62 were confirmed AN postoperatively by biopsy, whereas others are not AN. The area under the curve (AUC) of MRI was 0.611, whereas the AUC of ONT was 0.708. In the early-stage group, the AUC of MRI was 0.539, and the AUC of ONT was 0.744.

Conclusions: ONT was able to identify more subjects affected by unilateral incipient AN than MRI preoperatively. Given that ONT is a functional test for internal auditory canal nerves, it is an optimal screening test for AN patients because it provides more information than MRI for the further clinical plan. It is particularly noteworthy for identifying asymptomatic AN patients and for early stage. Therefore, it may help more patients from unnessesary surgery. Furthermore, an MRI follow-up is suggested if the patient was found alert in ONT.
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http://dx.doi.org/10.3389/fonc.2021.626485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276692PMC
June 2021

Modulation of Sirt1 and FoxO1 on Hypothalamic Leptin-Mediated Sympathetic Activation and Inflammation in Diet-Induced Obese Rats.

J Am Heart Assoc 2021 Jul 14;10(14):e020667. Epub 2021 Jul 14.

Division of Basic Biomedical Sciences Sanford School of Medicine of the University of South Dakota Vermillion SD.

Background Hypothalamic leptin-mediated signaling contributes to the exaggerated sympatho-excitation and increased blood pressure in obesity-associated hypertension. The aim of the study was to investigate the roles of energy-sensing enzyme sirtuin1 (Sirt1) and forkhead box protein O1 (FoxO1) on the hypothalamic leptin-mediated high sympathetic nerve activity and inflammation in obesity. Methods and Results Sprague Dawley rats were fed with high-fat diet (HFD) for 12 weeks. In vivo, the potential of Srit1 and FoxO1 in the sympathetic effects of leptin was investigated via siRNA injection to knockdown Sirt1 or FoxO1 gene in the arcuate nucleus (ARCN) of hypothalamus in rats. In vitro, the effects of Sirt1 or FoxO1 on leptin-mediated inflammation were observed in proopiomelanocortin (POMC) and microglial cells. Knockdown Sirt1 by siRNA significantly reduced the renal sympathetic nerve activity (RSNA) and blood pressure responses to leptin injection in the ARCN in the HFD rats. Conversely, knockdown FoxO1 significantly enhanced the RSNA and blood pressure responses to leptin injection in the HFD rats. Knockdown Sirt1 reduced the levels of pro-inflammatory cytokines interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), C1q/TNF-related protein-1 (CTRP1), and immune cell infiltration in the ARCN in the HFD rats. Knockdown FoxO1 significantly increased the level of IL-6 in the ARCN of HFD rats. In cultured hypothalamic POMC and microglial cells, knockdown Sirt1 significantly reduced leptin-induced IL-6 expression, affected the levels of AMP-activated protein kinase (AMPK) and serine/threonine-specific protein kinase (Akt). Knockdown FoxO1 significantly increased leptin-induced IL-6 in both POMC cells and microglial cells. Conclusions These data suggest that both Sirt1 and FoxO1 are the key modulators of leptin signaling in the hypothalamus contributed to the over sympathetic activation and inflammation in obesity.
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http://dx.doi.org/10.1161/JAHA.120.020667DOI Listing
July 2021

Correlation of Component Blood Transfusion of Hypertension Patients with Pregnancy and Postpartum Hemorrhage.

Clin Lab 2021 Jul;67(7)

Background: To analyze the correlation of component blood transfusion and prognosis of patients with pregnancy-induced hypertension (PIH) and postpartum hemorrhage (PPH) in assisted reproductive technology (ART).

Methods: A retrospective analysis of clinical data on 85 pregnant women with ART-induced PIH and PPH was completed. They were divided into observation group and control group based on whether there was component transfusion after massive hemorrhage. The plasma coagulation and blood platelet-related indexes of the two groups of patients were assayed before blood transfusion, 24 hours after blood transfusion, and 12 weeks post-partum. The incidence of adverse reactions of blood transfusion within one week of blood transfusion, the proportion of patients transferred to the general ward, the blood pressure, and biochemical indexes at 12 weeks postpartum were recorded.

Results: There was no significant difference in coagulation function before blood transfusion between the two groups of patients (p > 0.05). After 24 hours of massive blood transfusion, APTT, PT, and TT in the observation group were obviously shorter than those in the control group (p < 0.001), whereas FIB and PLT were higher than the control group (p < 0.01). In contrast to the control group at 12 weeks postpartum, the APTT, TT, and PT of the observation group were shortened (p < 0.05), while the FIB level was higher (p < 0.01). The probability of adverse reactions of transfusion in the observation group was lower than the control group (p < 0.05). The correlation between component transfusion and disease improvement after one week of transfusion and 12 weeks postpartum was 0.350 (0.159 - 0.530) (p < 0.01) and 0.441 (0.258 - 0.608) (p < 0.001).

Conclusions: Component blood transfusion in ART-induced PIH patients can improve their coagulation function, reduce the probability of adverse reactions caused by massive blood transfusion, and improve their prognosis.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200849DOI Listing
July 2021

A Noninvasive Multianalytical Approach for Lung Cancer Diagnosis of Patients with Pulmonary Nodules.

Adv Sci (Weinh) 2021 07 7;8(13):2100104. Epub 2021 May 7.

Department of Thoracic Surgery, Xinqiao Hospital Third Military Medical University (Army Medical University) Xinqiao Main Street Chongqing 400037 China.

Addressing the high false-positive rate of conventional low-dose computed tomography (LDCT) for lung cancer diagnosis, the efficacy of incorporating blood-based noninvasive testing for assisting practicing clinician's decision making in diagnosis of pulmonary nodules (PNs) is investigated. In this prospective observative study, next generation sequencing- (NGS-) based cell-free DNA (cfDNA) mutation profiling, NGS-based cfDNA methylation profiling, and blood-based protein cancer biomarker testing are performed for patients with PNs, who are diagnosed as high-risk patients through LDCT and subsequently undergo surgical resections, with tissue sections pathologically examined and classified. Using pathological classification as the gold standard, statistical and machine learning methods are used to select molecular markers associated with tissue's malignant classification based on a 98-patient discovery cohort (28 benign and 70 malignant), and to construct an integrative multianalytical model for tissue malignancy prediction. Predictive models based on individual testing platforms have shown varying levels of performance, while their final integrative model produces an area under the receiver operating characteristic curve (AUC) of 0.85. The model's performance is further confirmed on a 29-patient independent validation cohort (14 benign and 15 malignant, with power > 0.90), reproducing AUC of 0.86, which translates to an overall sensitivity of 80% and specificity of 85.7%.
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http://dx.doi.org/10.1002/advs.202100104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261512PMC
July 2021

Integration of FGF21 Signaling and Metabolomics in High-Fat Diet-Induced Obesity.

J Proteome Res 2021 Jul 8. Epub 2021 Jul 8.

Institute of Metabonomics & Medical NMR, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.

Sex differences in obesity have been well established, but the metabolic mechanism underlying these differences remains unclear. In the present study, we determined the expression levels of endogenous fibroblast growth factor 21 (FGF21) and its related receptors in male and female mice that were fed a high-fat diet (HFD) for 12 weeks. We also analyzed the metabolic changes in serum and livers using a nuclear magnetic resonance-based metabolomics approach. Reverse transcription polymerase chain reaction and western blotting results revealed that the levels of FGFR1, FGFR2, and co-factor β-klotho were upregulated in female mice to alleviate FGF21 resistance induced by HFD. The metabolomics results demonstrated that the serum and liver metabolic patterns of HFD-fed male mice were significantly separated from those of the female HFD-fed group and the normal diet group. Furthermore, low-density lipoprotein/very low density lipoprotein and betaine levels were associated with the resistance of exogenous HFD in female mice. These findings imply that sex-based differences in metabolism and susceptibility to obesity might be mediated by the FGF21 signaling pathway.
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http://dx.doi.org/10.1021/acs.jproteome.1c00197DOI Listing
July 2021

Distinct Extracellular RNA Profiles in Different Plasma Components.

Front Genet 2021 21;12:564780. Epub 2021 Jun 21.

Department of Tumor Biology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, United States.

Circulating extracellular RNAs (exRNAs) have great potential to serve as biomarkers for a wide range of diagnostic, therapeutic, and prognostic applications. So far, knowledge of the difference among different sources of exRNAs is limited. To address this issue, we performed a sequential physical and biochemical precipitation to collect four fractions (platelets and cell debris, the thrombin-induced precipitates, extracellular vesicles, and supernatant) from each of 10 plasma samples. From total RNAs of the 40 fractions, we prepared ligation-free libraries to profile full spectrum of all RNA species, without size selection and rRNA reduction. Due to complicated RNA composition in these libraries, we utilized a successive stepwise alignment strategy to map the RNA sequences to different RNA categories, including miRNAs, piwi-interacting RNAs, tRNAs, rRNAs, lincRNAs, snoRNAs, snRNAs, other ncRNAs, protein coding RNAs, and circRNAs. Our data showed that each plasma fraction had its own unique distribution of RNA species. Hierarchical cluster analyses using transcript abundance demonstrated similarities in the same plasma fraction and significant differences between different fractions. In addition, we observed various unique transcripts, and novel predicted miRNAs among these plasma fractions. These results demonstrate that the distribution of RNA species and functional RNA transcripts is plasma fraction-dependent. Appropriate plasma preparation and thorough inspection of different plasma fractions are necessary for an exRNA-based biomarker study.
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http://dx.doi.org/10.3389/fgene.2021.564780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256274PMC
June 2021

Pathogenic Species Are Associated with Distinct Environmental Niches and Planktonic Taxa in Southern California (USA) Aquatic Microbiomes.

mSystems 2021 Jul 6:e0057121. Epub 2021 Jul 6.

Scripps Institution of Oceanography, University of California San Diego, La Jolla, California, USA.

Interactions between vibrio bacteria and the planktonic community impact marine ecology and human health. Many coastal spp. can infect humans, representing a growing threat linked to increasing seawater temperatures. Interactions with eukaryotic organisms may provide attachment substrate and critical nutrients that facilitate the persistence, diversification, and spread of pathogenic spp. However, vibrio interactions with planktonic organisms in an environmental context are poorly understood. We quantified the pathogenic species V. cholerae, V. parahaemolyticus, and V. vulnificus monthly for 1 year at five sites and observed high abundances, particularly during summer months, with species-specific temperature and salinity distributions. Using metabarcoding, we established a detailed profile of both prokaryotic and eukaryotic coastal microbial communities. We found that pathogenic species were frequently associated with distinct eukaryotic amplicon sequence variants (ASVs), including diatoms and copepods. Shared environmental conditions, such as high temperatures and low salinities, were associated with both high concentrations of pathogenic vibrios and potential environmental reservoirs, which may influence vibrio infection risks linked to climate change and should be incorporated into predictive ecological models and experimental laboratory systems. Many species of coastal vibrio bacteria can infect humans, representing a growing health threat linked to increasing seawater temperatures. However, their interactions with surrounding microbes in the environment, especially eukaryotic organisms that may provide nutrients and attachment substrate, are poorly understood. We quantified three pathogenic species monthly for a duration of 1 year, finding that all three species were abundant and exhibited species-specific temperature and salinity distributions. Using metabarcoding, we investigated associations between these pathogenic species and prokaryotic and eukaryotic microbes, revealing genus and amplicon sequence variant (ASV)-specific relationships with potential functional implications. For example, pathogenic species were frequently associated with chitin-producing eukaryotes, such as diatoms in the genus and copepods. These associations between high concentrations of pathogenic vibrios and potential environmental reservoirs should be considered when predicting infection risk and developing ecologically relevant model systems.
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http://dx.doi.org/10.1128/mSystems.00571-21DOI Listing
July 2021

Real-World Experience of Olaparib Treatment in Patients with Ovarian Cancer: A Chinese Multicenter Study.

Mol Cancer Ther 2021 Jun 17. Epub 2021 Jun 17.

Beijing Shijitan Hospital, Capital Medical University, Peking University Ninth School of Clinical Medicine.

The objective of this study was to evaluate the real-world application, efficacy, and safety data of Olaparib for maintenance therapy and active treatment in ovarian cancer patients in China. Ovarian cancer patients from 17 institutions in China treated with Olaparib as maintenance or active therapy from January 2018 to March 2020 were included in this study. The medical records were reviewed, and follow-up information was collected for analysis of the patients' clinicopathological characteristics as well as the effectiveness and safety of Olaparib. A total of 251 patients receiving Olaparib were included, with 84 as maintenance therapy after first-line chemotherapy (FL-M), 97 as maintenance therapy after platinum-sensitive recurrence (PSR-M), and 70 as active treatment (AT). The probability of progression-free survival (PFS) at 12 months was 87.6% in the FL-M group and 63.8% in the PSR-M group. According to the multivariate analysis, complete response (CR) to chemotherapy for the PSR-M patients was the only factor affecting the PFS (HR = 0.414, P = 0.014), and platinum-sensitivity was the only factor affecting PFS improvement in the AT group (HR = 0.317, P = 0.009). In the AT group, the objective response rate was 37.1%, the CR rate was 7.1%, and 30% of the patients had stable disease. Eight (3.2%) patients discontinued Olaparib due to toxicity. Anemia was the most common adverse event. In conclusion, Olaparib is effective and well-tolerated in the real-world setting of ovarian cancer treatment. Platinum sensitivity is positively correlated to the effectiveness of Olaparib in both maintenance and active treatment.
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http://dx.doi.org/10.1158/1535-7163.MCT-20-1064DOI Listing
June 2021

SHP2 inhibition enhances the anticancer effect of Osimertinib in EGFR T790M mutant lung adenocarcinoma by blocking CXCL8 loop mediated stemness.

Cancer Cell Int 2021 Jul 3;21(1):337. Epub 2021 Jul 3.

Basic College of Medicine, Anhui Medical University, 81 Meishan road, Hefei, Anhui, China.

Background: Additional epidermal growth factor receptor (EGFR) mutations confer the drug resistance to generations of EGFR targeted tyrosine kinase inhibitor (EGFR-TKI), posing a major challenge to developing effective treatment of lung adenocarcinoma (LUAD). The strategy of combining EGFR-TKI with other synergistic or sensitizing therapeutic agents are considered a promising approach in the era of precision medicine. Moreover, the role and mechanism of SHP2, which is involved in cell proliferation, cytokine production, stemness maintenance and drug resistance, has not been carefully explored in lung adenocarcinoma (LUAD).

Methods: To evaluate the impact of SHP2 on the efficacy of EGFR T790M mutant LUAD cells to Osimertinib, SHP2 inhibition was tested in Osimertinib treated LUAD cells. Cell proliferation and stemness were tested in SHP2 modified LUAD cells. RNA sequencing was performed to explore the mechanism of SHP2 promoted stemness.

Results: This study demonstrated that high SHP2 expression level correlates with poor outcome of LUAD patients, and SHP2 expression is enriched in Osimertinib resistant LUAD cells. SHP2 inhibition suppressed the cell proliferation and damaged the stemness of EGFR T790M mutant LUAD. SHP2 facilitates the secretion of CXCL8 cytokine from the EGFR T790M mutant LUAD cells, through a CXCL8-CXCR1/2 positive feedback loop that promotes stemness and tumorigenesis. Our results further show that SHP2 mediates CXCL8-CXCR1/2 feedback loop through ERK-AKT-NFκB and GSK3β-β-Catenin signaling in EGFR T790M mutant LUAD cells.

Conclusions: Our data revealed that SHP2 inhibition enhances the anti-cancer effect of Osimertinib in EGFR T790M mutant LUAD by blocking CXCL8-CXCR1/2 loop mediated stemness, which may help provide an alternative therapeutic option to enhance the clinical efficacy of osimertinib in EGFR T790M mutant LUAD patients.
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http://dx.doi.org/10.1186/s12935-021-02056-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254369PMC
July 2021

Investigation of α-Synuclein Species in Plasma Exosomes and the Oligomeric and Phosphorylated α-Synuclein as Potential Peripheral Biomarker of Parkinson's Disease.

Neuroscience 2021 08 27;469:79-90. Epub 2021 Jun 27.

Department of Medical Genetics & Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China. Electronic address:

α-Synuclein (α-syn), especially its abnormal oligomeric and phosphorylated form, plays a critical role in the pathogenesis of Parkinson's disease (PD). Plasma exosomal α-syn species have been shown to be a promising PD biomarker. However, whether different α-syn species in plasma exosomes (the oligomeric α-syn and the Ser129 phosphorylated α-syn (p-α-syn)) which represent the PD pathogenesis in the brain could be specific peripheral PD biomarker haven't been well elucidated. In this study, we successfully extracted and identified the human plasma exosomes, and the CNS-derived exosomes were detected. The different aggregation status, localization and degradation characteristics of α-syn and p-α-syn in the plasma exosomes between PD patients and healthy controls were further analyzed. The results suggested that α-syn and p-α-syn in the plasma exosomes of PD patients showed poor solubility after protease K (PK) treatment. Aggregated α-syn and p-α-syn existed both inside and on the membrane surface of plasma exosomes. The Receiver operating characteristic (ROC) performance of α-syn oligomer/total α-syn in exosomes was moderately helpful in PD diagnosis (AUC = 0.71, sensitivity = 60.5%, specificity = 59.4%), and the ratio of p-α-syn oligomer/total p-α-syn showed similar result (AUC = 0.69, sensitivity = 60.0%, specificity = 59.5%). This study indicates that the oligomeric α-syn/total α-syn and oligomeric p-α-syn/total p-α-syn ratio in plasma exosomes may be applied to assist the PD diagnosis, which needs further research.
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http://dx.doi.org/10.1016/j.neuroscience.2021.06.033DOI Listing
August 2021

New Biotransformation Mode of Zearalenone Identified in Y816 Revealing a Novel ZEN Conjugate.

J Agric Food Chem 2021 Jul 28;69(26):7409-7419. Epub 2021 Jun 28.

National Technology Innovation Center of Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

An increasing number of strains have been identified, and the removal capacity of zearalenone (ZEN) was determined; however, they failed to reveal the detoxification mechanism and transformation product. Here, Y816, which could transform 40 mg/L of ZEN within 7 h of fermentation, was identified and studied. First, the biotransformation products of ZEN and 17-β-estradiol (E2) were identified as ZEN-14-phosphate and E2-3-phosphate by HPLC-TOF-MS and NMR, respectively. An intracellular zearalenone phosphotransferase (ZPH) was found through transcriptome sequencing analysis of Y816. The phosphorylated reaction conditions of ZEN by ZPH were further revealed in this work. Furthermore, the phosphorylated conjugates showed reduced estrogenic toxicity compared with their original substances (ZEN and α/β-zearalenol) using an engineered yeast biosensor system. The first report on the phosphorylated conjugated mode of ZEN in Y816 will inspire new perspectives on the biotransformation of ZEN in strains.
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http://dx.doi.org/10.1021/acs.jafc.1c01817DOI Listing
July 2021

Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells.

J Hazard Mater 2021 Jun 12;419:126378. Epub 2021 Jun 12.

Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou 350122, China; Fujian Provincial Key Laboratory of Environmental Factors and Cancer, School of Public Health, Fujian Medical University, Fuzhou 350122, China; The Key Laboratory of Environment and Health, School of Public Health, Fujian Medical University, Fuzhou 350122, China. Electronic address:

Cobalt is a hazardous material that has harmful effects on neurotoxicity. Excessive exposure to cobalt or inactivation of the unique proline isomerase Pin1 contributes to age-dependent neurodegeneration. However, nothing is known about the role of Pin1 in cobalt-induced neurodegeneration. Here we find that out of several hazardous materials, only cobalt dose-dependently decreased Pin1 expression and alterations in its substrates, including cis and trans phosphorylated Tau in human neuronal cells, concomitant with neurotoxicity. Cobalt-induced neurotoxicity was aggravated by Pin1 genetic or chemical inhibition, but rescued by Pin1 upregulation. Furthermore, less than 4 μg/l of blood cobalt induced dose- and age-dependent Pin1 downregulation in murine brains, ensuing neurodegenerative changes. These defects were corroborated by changes in Pin1 substrates, including cis and trans phosphorylated Tau, amyloid precursor protein, β amyloid and GSK3β. Moreover, blood Pin1 was downregulated in human hip replacement patients with median blood cobalt level of 2.514 μg/l, which is significantly less than the safety threshold of 10 μg/l, suggesting an early role Pin1 played in neurodegenerative damages. Thus, Pin1 inactivation by cobalt contributes to age-dependent neurodegeneration, revealing that cobalt is a hazardous material triggering AD-like neurodegenerative damages.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126378DOI Listing
June 2021

Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice.

Microbiome 2021 06 25;9(1):145. Epub 2021 Jun 25.

Institute of Metabonomics & Medical NMR, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China.

Background: Modification of the gut microbiota has been reported to reduce the incidence of type 1 diabetes mellitus (T1D). We hypothesized that the gut microbiota shifts might also have an effect on cognitive functions in T1D. Herein we used a non-absorbable antibiotic vancomycin to modify the gut microbiota in streptozotocin (STZ)-induced T1D mice and studied the impact of microbial changes on cognitive performances in T1D mice and its potential gut-brain neural mechanism.

Results: We found that vancomycin exposure disrupted the gut microbiome, altered host metabolic phenotypes, and facilitated cognitive impairment in T1D mice. Long-term acetate deficiency due to depletion of acetate-producing bacteria resulted in the reduction of synaptophysin (SYP) in the hippocampus as well as learning and memory impairments. Exogenous acetate supplement or fecal microbiota transplant recovered hippocampal SYP level in vancomycin-treated T1D mice, and this effect was attenuated by vagal inhibition or vagotomy.

Conclusions: Our results demonstrate the protective role of microbiota metabolite acetate in cognitive functions and suggest long-term acetate deficiency as a risk factor of cognitive decline. Video Abstract.
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http://dx.doi.org/10.1186/s40168-021-01088-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235853PMC
June 2021

An early assessment of a case fatality risk associated with P.1 SARS-CoV-2 lineage in Brazil: an ecological study.

J Travel Med 2021 Jun 21. Epub 2021 Jun 21.

JC School of Public Health and Primary Care, Chinese University of Hong Kong, Hong Kong, China.

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http://dx.doi.org/10.1093/jtm/taab078DOI Listing
June 2021

Synthesis and characterization of bulk Nd Sr NiO and Nd Sr NiO.

Phys Rev Mater 2020 Aug;4(8)

Materials Science Division, Argonne National Laboratory, Lemont, Illinois 60439, USA.

The recent reports of superconductivity in Nd Sr NiO/SrTiO heterostructures have reinvigorated interest in potential superconductivity of low-oxidation state nickelates. Synthesis of Ni-containing compounds is notoriously difficult. In the current work, a combined sol-gel combustion and high-pressure annealing technique was employed to prepare polycrystalline perovskite Nd Sr NiO ( = 0, 0.1, and 0.2). Metal nitrates and metal acetates were used as starting materials, and the latter were found to be superior to the former in terms of safety and reactivity. The Nd Sr NiO compounds were subsequently reduced to Nd Sr NiO using calcium hydride in a sealed, evacuated quartz tube. To understand the synthesis pathway, the evolution from NdNiO to NdNiO was monitored using synchrotron x-ray diffraction during the reduction process. Electrical transport properties were consistent with an insulator-metal transition occurring between = 0 and 0.1 for Nd Sr NiO. Superconductivity was not observed in our bulk samples of Nd Sr NiO. Neutron diffraction experiments at 3 and 300 K were performed on NdSrNiO, in which no magnetic Bragg reflections were observed, and the results of structural Rietveld refinement are provided.
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http://dx.doi.org/10.1103/PhysRevMaterials.4.084409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207457PMC
August 2020

Rosiglitazone restores nitric oxide synthase-dependent reactivity of cerebral arterioles in rats exposed to prenatal alcohol.

Alcohol Clin Exp Res 2021 Jul 12;45(7):1359-1369. Epub 2021 Jun 12.

Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD, USA.

Background: Prenatal exposure to alcohol leads to a greater incidence of many cardiovascular-related diseases, presumably via a mechanism that may involve increased oxidative stress. An agonist of peroxisome proliferator-activated receptor gamma (PPARγ; rosiglitazone) has been shown to suppress alcohol-induced neuroinflammation and oxidative stress. The goal of this study was to determine whether acute and chronic treatment with rosiglitazone could restore or prevent impaired nitric oxide synthase (NOS)-dependent responses of cerebral arterioles in male and female adult (14-16 weeks old) rats exposed to alcohol in utero.

Methods: We fed Sprague-Dawley dams a liquid diet with or without 3% ethanol for the duration of their pregnancy (21-23 days). In the first series of studies, we examined the reactivity of cerebral arterioles to eNOS- (ADP), nNOS-dependent (NMDA), and NOS-independent agonists in male and female adult rats before and during acute (1 hour) topical application of rosiglitazone (1 µM). In a second series of studies, we examined the influence of chronic treatment with rosiglitazone (3 mg/kg/day in drinking water for 2-3 weeks) on the responses of cerebral arterioles in male and female adult rats exposed to alcohol in utero.

Results: We found that in utero exposure to alcohol similarly reduced responses of cerebral arterioles to ADP and NMDA, but not to nitroglycerin in male and female adult rats. In addition, acute treatment of the male and female adult rats with rosiglitazone similarly restored this impairment in cerebral vascular function to that observed in controls. We also found that chronic treatment with rosiglitazone prevented impaired vascular function in male and female adult rats that were exposed to alcohol in utero.

Conclusions: PPARγ activation may be an effective and relevant treatment to reverse or prevent cerebral vascular abnormalities associated with prenatal exposure to alcohol.
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http://dx.doi.org/10.1111/acer.14634DOI Listing
July 2021

Improving SERS Sensitivity toward Trace Sulfonamides: The Key Role of Trade-Off Interfacial Interactions among the Target Molecules, Anions, and Cations on the SERS Active Surface.

Anal Chem 2021 06 11;93(24):8603-8612. Epub 2021 Jun 11.

State Key Laboratory of Marine Environmental Science, Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies, Center for Marine Environmental Chemistry & Toxicology, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China.

In recent years, ensuring the rational use and effective control of antibiotics has been a major focus in the eco-environment, which requires an effective monitoring method. However, on-site rapid detection of antibiotics in water environments remains a challenging issue. In this study, surface-enhanced Raman spectroscopy (SERS) was used to systematically achieve selective, rapid, and highly sensitive detection of sulfonamides, based on their fingerprint characteristics. The results show that the trade-off between the competitive and coadsorption behaviors of target molecules and agglomerates (inorganic salts) on the surface of the SERS substrate determines whether the molecules can be detected with high sensitivity. Based on this, the qualitative differentiation and quantitative detection of three structurally similar antibiotics, sulfadiazine, sulfamerazine, and sulfamethazine, were achieved, with the lowest detectable concentration being 1 μg/L for sulfadiazine and 50 μg/L for sulfamerazine and sulfamethazine.
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http://dx.doi.org/10.1021/acs.analchem.1c01530DOI Listing
June 2021

The effects of short time hyperoxia on glutamate concentration and glutamate transporters expressions in brain of neonatal rats.

Neurosci Lett 2021 07 7;758:136013. Epub 2021 Jun 7.

Neonatology Department, Anhui Provincial Children's Hospital, Hefei, China.

Preterm infants often suffer from impaired postnatal brain development, and glutamate excitotoxicity is identified as a pivotal mechanism of hyperoxia-induced neurological abnormality. We aimed to investigate the effect of short time hyperoxia on glutamate homeostasis and glutamate transporters expressions in immature brain. Six-day-old (P6) rat pups were exposed to 80% oxygen for 24 h (the hyperoxia group) or placed in atmospheric air (the control group). The concentrations of glutamate and γ-aminobutyric acid (GABA) in immature cerebrum and cerebellum at P7, P14 and P21 were determined by ELISA. The mRNA levels of glutamate transporters including excitatory amino acid transporter 1 (EAAT1), EAAT2, EAAT3, vesicular glutamate transporter 1 (VGLUT1) and VGLUT2 in brain were determined by qPCR. Glutamate accumulation was induced by hyperoxia both in immature cerebrum and cerebellum at P7 but got gradually attenuated at P14 and P21, as evidenced by the changes of glutamate and GABA concentrations. Hyperoxia also induced sustained glutamatic oxidative stress in both cerebrum and cerebellum, as GSH (reduced glutathione) levels in the hyperoxia group were constantly higher than the control group at three examined time-points. Furthermore, at P7, the expressions of all glutamate transporters decreased in both cerebrum and cerebellum except that of EAAT1. At P21, VGLUT2 in cerebrum and EAAT1, EAAT3 and VGLUT2 in cerebellum still displayed significant decrease in expression levels upon hyperoxia stimulation. Taken together, our results indicate that hyperoxia induces glutamate accumulation in brain of rat pups, which is associated with increased oxidative stress and decreased expressions of glutamate transporters.
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http://dx.doi.org/10.1016/j.neulet.2021.136013DOI Listing
July 2021

Pf77 and male development gene 1 as vaccine antigens that induce potent transmission-reducing antibodies.

Sci Transl Med 2021 06;13(597)

Laboratory of Emerging Pathogens, Division of Emerging and Transfusion Transmitted Diseases, Office of Blood Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA.

Malaria vaccines that disrupt the life cycle in mosquitoes and reduce parasite transmission in endemic areas are termed transmission-blocking vaccines (TBVs). Despite decades of research, there are only a few antigens that indisputably and reproducibly demonstrate transmission-blocking immunity. So far, only two TBV candidates have advanced to phase 1/2 clinical testing with limited success. By applying an unbiased transcriptomics-based approach, we have identified Pf77 and male development gene 1 (PfMDV-1) as two TBV antigens that, upon immunization, induced antibodies that caused reductions in oocyst counts in mosquito midguts in a standard membrane feeding assay. In-depth studies were performed to characterize the genetic diversity of, stage-specific expression by, and natural immunity to these two molecules to evaluate their suitability as TBV candidates. Pf77 and PfMDV-1 display limited antigenic polymorphism, are pan-developmentally expressed within the parasite, and induce naturally occurring antibodies in Ghanaian adults, which raises the prospect of natural boosting of vaccine-induced immune response in endemic regions. Together, these biological properties suggest that Pf77 and PfMDV-1 may warrant further investigation as TBV candidates.
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http://dx.doi.org/10.1126/scitranslmed.abg2112DOI Listing
June 2021

Asphyxia and Neonatal Respiratory Distress Syndrome Are Independent Predictors of the Non-response to Inhaled Nitric Oxide in the Newborns With PPHN.

Front Pediatr 2021 20;9:665830. Epub 2021 May 20.

Neonatology Department, Anhui Provincial Children Hospital, Hefei, China.

Not all the neonates respond with improvement in oxygenation following inhaled nitric oxide treatment (iNO) treatment. The aim of this study was to assess the independent risk factors associated with non-response to iNO during the 2 weeks of postnatal treatment in neonates diagnosed with persistent pulmonary hypertension (PPHN). This retrospective cohort study included all newborns with PPHN who received iNO treatment for more than 24 h. Demographic, obstetric, perinatal data and clinical complications were extracted from the hospitalization records. Subjects were divided into two groups according to their response to iNO inspiration during the first 24 h of iNO treatment. No response was defined as an increase in SpO < 5% or the inability to sustain saturation levels in the first 24 h of iNO treatment. For descriptive statistics, χ and -test analysis were used to compare categorical and continuous variables between the two groups. To evaluate independent risk factors of non-responsiveness to iNO treatment, binary logistic regression analysis were performed. A total of 75 newborns were included in the study. Sixty-two cases were in the responders group, and 13 cases were in the non-responders group. Univariate analysis showed that asphyxia, neonatal respiratory distress syndrome (NRDS), pulmonary surfactant administration, meconium aspiration syndrome (MAS), the severity of pulmonary hypertension (PH), and high-frequency oscillatory ventilation (HFOV) therapy were the high-risk factors affecting the response to iNO treatment in the newborns with PPHN. The binary logistic regression analysis indicated that asphyxia and NRDS incidence were independent predictors of non-responsiveness to iNO treatment [asphyxia: OR 4.193, 95% CI 1.104-15.927, = 0.035; NRDS: OR 0.154, 95% CI 0.036-0.647, = 0.011]. The patients in the non-responders group had shorter iNO inspiration followed by MV duration, supplemental oxygen and hospital stay, and higher mortality. There were no significant differences in IVH, PVL, and BPD between two groups. In the newborns with PPHN, asphyxia and NRDS resulted as the independent risk factors of non-responsiveness to iNO therapy. Asphyxia in the newborns with PPHN is detrimental to the response to iNO treatment, while NRDS is beneficial.
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http://dx.doi.org/10.3389/fped.2021.665830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172584PMC
May 2021

Programmable RNA N -Methyladenosine Demethylation by a Cas13d-Directed Demethylase.

Angew Chem Int Ed Engl 2021 Jun 3. Epub 2021 Jun 3.

Department of Cell Biology, College of Life Science, Sichuan Normal University, Chengdu, Sichuan, 610101, China.

N -methyladenosine (m A) is a prevalent and reversible RNA modification, which plays a crucial role in the regulation of RNA fate and gene expression. However, the lack of tools to precisely manipulate m A sites in specific transcripts has hindered efforts to clarify the association between a specific m A-modified transcript and its phenotypic outcomes. Here we develop a CRISPR-Cas13d-based tool called reengineered m A modification valid eraser (termed "REMOVER") for targeted m A demethylation of a specific transcript. The catalytically inactive RfxCas13d (dCasRx) is fused to the m A demethylase ALKBH3, and the dCasRx-ALKBH3 fusion protein can mediate potent demethylation of m A-modified RNAs. We further find that REMOVER can specifically demethylate m A of MALAT1 and PRUNE1 RNAs, thereby significantly increasing their stability. Our study establishes REMOVER as a tool for targeted RNA demethylation of specific m A-modified transcripts, which enables further elucidation of the relationship between m A modification of specific transcripts and their phenotypic outcomes.
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http://dx.doi.org/10.1002/anie.202105253DOI Listing
June 2021

Investigation and Analysis of the Colonization and Prevalence of Carbapenem-Resistant in Pediatric Liver Transplant Recipients.

Infect Drug Resist 2021 25;14:1957-1966. Epub 2021 May 25.

Organ Transplantation Center, Tianjin First Central Hospital, Tianjin, 300192, People's Republic of China.

Objective: This study aimed to investigate the colonization and prevalence of carbapenem-resistant (CRE) in pediatric liver transplant recipients and analyze the high-risk factors and prognosis of CRE infection.

Methods: A prospective study involving 152 pediatric patients undergoing liver transplantation was carried out. Anal swab bacteria cultures were collected when the patients entered the intensive care unit (ICU) and when they left in order to screen for intestinal CRE colonization. The results were grouped according to the occurrence of CRE infection following surgery, and the patients were divided into two groups: a CRE infection group and a non-CRE infection group. Univariate analysis and multiple logistic regression analysis were conducted to determine the independent risk factors of CRE infection and analyze the survival rate.

Results: Of the 152 pediatric liver transplant recipients enrolled in the study, there were 13 cases of postoperative CRE infection and 139 cases of non-CRE infection. The incidence of preoperative CRE infection, preoperative cytomegalovirus (CMV) infection, and preoperative sepsis in the CRE infection group was significantly higher than in the non-CRE infection group ( < 0.005). Intraoperative bleeding volume and operation times in the CRE infection group were also significantly higher than in the non-CRE infection group ( < 0.05). Furthermore, postoperative ICU treatment time, postoperative occurrence of unplanned surgery, postoperative mechanical ventilation of more than 24 hours, and the incidence of pre-ICU CRE colonization in the CRE infection group were significantly higher than in the non-CRE infection group ( < 0.05). Finally, the difference between the CRE infection group and the non-CRE infection group in six-month survival rate following surgery was significant ( < 0.001).

Conclusion: The independent risk factors of CRE infection following pediatric liver transplantation include preoperative CRE infection and pre-ICU CRE colonization. CRE infection progresses quickly, with a poor prognosis and a high mortality rate. The CRE screening of anal swabs is crucial for the early detection of CRE infection.
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http://dx.doi.org/10.2147/IDR.S304998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164869PMC
May 2021

A novel clinically relevant graft-versus-leukemia model in humanized mice.

J Leukoc Biol 2021 May 31. Epub 2021 May 31.

Penn State Cancer Institute, Penn State University College of Medicine, Hershey, Pennsylvania, USA.

The prognosis for acute myeloid leukemia (AML) relapse post allogeneic hematopoietic stem cell transplantation (alloSCT) is dismal. Novel effective treatment is urgently needed. Clinical benefit of alloSCT greatly relies on the graft-versus-leukemia (GVL) effect. The mechanisms that mediate immune escape of leukemia (thus causing GVL failure) remain poorly understood. Studies of human GVL have been hindered by the lack of optimal clinically relevant models. Here, using our large, longitudinal clinical tissue bank that include AML cells and G-CSF mobilized donor hematopoietic stem cells (HSCs), we successfully established a novel GVL model in humanized mice. Donor HSCs were injected into immune-deficient NOD-Cg-Prkdc IL2rg /SzJ (NSG) mice to build humanized mice. Immune reconstitution in these mice recapitulated some clinical scenario in the patient who received the corresponding HSCs. Allogeneic but HLA partially matched patient-derived AML cells were successfully engrafted in these humanized mice. Importantly, we observed a significantly reduced (yet incomplete elimination of) leukemia growth in humanized mice compared with that in control NSG mice, demonstrating a functional (but defective) GVL effect. Thus, for the first time, we established a novel humanized mouse model that can be used for studying human GVL responses against human AML cells in vivo. This novel clinically relevant model provides a valuable platform for investigating the mechanisms of human GVL and development of effective leukemia treatments.
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http://dx.doi.org/10.1002/JLB.5AB0820-542RRDOI Listing
May 2021

Diagnostic accuracy of the Xpert MTB/RIF assay for tuberculous pericarditis: A protocol of systematic review and meta-analysis.

PLoS One 2021 26;16(5):e0252109. Epub 2021 May 26.

Department of Thoracic Surgery, Zhejiang Chinese Medicine and Western Medicine Integrated Hospital, Zhejiang, China.

Background: Tuberculous pericarditis (TBP) can lead to serious consequences. Early diagnosis and treatment are very important for TBP, but early diagnosis is still very challenging. This study aims to evaluate the diagnostic accuracy of Xpert MTB/RIF for TBP using meta-analysis method.

Methods: We will search Embase, PubMed, the Cochrane Library, China National Knowledge Infrastructure (CNKI), and the Wanfang database for researches assessing the diagnostic accuracy of Xpert MTB/RIF for TBP until April 2021. Any types of study design with full text will be selected and included. The Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool will be used to assess the risk of bias. We will use version 15.0 of the STATA software with the midas command packages to carry out meta-analyses.

Results: Evidence for diagnostic accuracy of Xpert MTB/RIF for TBP will be provided through the study, and this protocol will be submitted to a peer-reviewed journal for publication.

Conclusion: This study will provide evidence of Xpert MTB/RIF for TBP.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252109PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153464PMC
May 2021

Repositioning of Antiparasitic Drugs for Tumor Treatment.

Front Oncol 2021 29;11:670804. Epub 2021 Apr 29.

Department of Thoracic Surgery, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing, China.

Drug repositioning is a strategy for identifying new antitumor drugs; this strategy allows existing and approved clinical drugs to be innovatively repurposed to treat tumors. Based on the similarities between parasitic diseases and cancer, recent studies aimed to investigate the efficacy of existing antiparasitic drugs in cancer. In this review, we selected two antihelminthic drugs (macrolides and benzimidazoles) and two antiprotozoal drugs (artemisinin and its derivatives, and quinolines) and summarized the research progresses made to date on the role of these drugs in cancer. Overall, these drugs regulate tumor growth multiple targets, pathways, and modes of action. These antiparasitic drugs are good candidates for comprehensive, in-depth analyses of tumor occurrence and development. In-depth studies may improve the current tumor diagnoses and treatment regimens. However, for clinical application, current investigations are still insufficient, warranting more comprehensive analyses.
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http://dx.doi.org/10.3389/fonc.2021.670804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117216PMC
April 2021

Application Value of Lipoprotein-Associated Phospholipase A2 Expression Level in Evaluating the Risk of Major Bleeding in Gestational Diabetes.

Clin Lab 2021 May;67(5)

Background: Studies have shown that obesity and lipid metabolism disorders can lead to increased Lp-PLA2 ac-tivity in the body. However, few studies have reported the correlation between Lp-PLA2 and postpartum hemorrhage in pregnant women with GMD.

Methods: Clinical data was collected from 43 pregnant women with gestational diabetes combined with postpartum hemorrhage and 50 pregnant women with postpartum diabetes without postpartum bleeding during the same period. Lp-PLA2 expression levels in the serum of pregnant women in both groups were detected. The predictive value of Lp-PLA2 expression level for postpartum hemorrhage was clarified.

Results: (1) From 20w to 32w pregnancy, the serum Lp-PLA2 levels of pregnant women in both groups showed a gradual increasing trend. (2) The serum Lp-PLA2 level of the pregnant women in the research group was significantly higher (p < 0.001). (3) Binary logistic regression analysis shows that Lp-PLA2 level has a good correlation with postpartum major bleeding. (4) Comparing of Lp-PLA2 levels in different gestational weeks with respect to its efficacy for predicting postpartum hemorrhage found that the sensitivity and specificity of 24w Lp-PLA2 levels are higher than other gestational weeks, and the area under the 24w parameter curve (0.955) > 32w area under the parameter curve (0.952) > area under the 20w parameter curve (0.940) > area under the 28w parameter curve (0.887). (5) The birth weight of the newborn in the research group was significantly heavier (p < 0.001).

Conclusions: Dynamic monitoring of the expression level of Lp-PLA2 in the serum of pregnant women can predict the risk of postpartum hemorrhage in pregnant women at an early stage and help optimize the rescue plan for postpartum hemorrhage.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200906DOI Listing
May 2021

Population genomics study of Vibrio alginolyticus.

Yi Chuan 2021 Apr;43(4):350-361

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.

Vibrio alginolyticus is a Gram-negative bacillus that causes vibriosis to human and aquatic products, including fish, shrimp and shellfish. It poses a threat to public health and causes enormous economic losses to the aquaculture industry. However, research on genetic diversity and pathogenicity-related genetic elements based on whole genome is still lacking. In this study, sixty-eight strains of V. alginolyticus were collected from four provinces of China and the whole genome sequences were obtained. Combined with 113 publicly available genome sequences downloaded from NCBI, we inferred the population structure of V. alginolyticus by using fineSTRUCTURE software, and identified the virulence and antibiotic resistance factors using the VFDB, CARD and ResFinder database. The results indicated that V. alginolyticus included two main lineages, named Lineage 1 and Lineage 2. Both lineages distributed in America and Asia, but all the European genomes were classified into Lineage 1. A single cross-ocean transmission event was inferred from one of the 12 identified clonal groups in our dataset. V. alginolyticus genome contains a variety of virulence factors, such as tlh, OmpU, and IlpA, etc. The distribution of virulence factors revealed no lineage-specificity, but some of which revealed differences in their geographical distribution. A lower frequency of VP1611, vcrD, vopD, fleR/flrC and a higher frequency of IlpA were observed in genomes of Europe than other continents. In China, a lower frequency of fleR/flrC, and no IlpA were observed in genomes from Guangxi province. Among the identified antibiotic resistance genes, TxR and fos are significantly enriched in Lineage 2. In addition, TxR is more common in genomes from Asia, compared with the American and European genomes. But in China, the frequency of TxR in Sichuan genomes is much lower than in other provinces. We also found that large fragments of plasmids or ICEs that carried multiple drug resistance genes were present in five V. alginolyticus genomes (VA24, VA28, 2014V-1011, ZJ-T and Vb1833). Based on population genomics analysis, our study delineated the population structure, distribution of virulence and antibiotic resistance related factors of V. alginolyticus, which lays a foundation for future study of genetic characters and pathogenesis mechanism of this pathogen and will improve the works on monitoring, prevention and control of this pathogen.
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http://dx.doi.org/10.16288/j.yczz.21-061DOI Listing
April 2021