Publications by authors named "Hong Zhang"

5,191 Publications

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Chromosome-level assembly of the Hypophthalmichthys molitrix (Cypriniformes: Cyprinidae) genome provides insights into its ecological adaptation.

Genomics 2021 Jun 18. Epub 2021 Jun 18.

Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Changsha University, Changsha, China.

Hypophthalmichthys molitrix (silver carp) is phytoplanktivorous and is an economically and ecologically important fish species. As a well-known invasive species, a number of factors associated with the ecological adaptations of this species are largely unknown. Here, we present a chromosomal-level assembly of the species based on the PacBio Sequel II platform and Hi-C scaffolding technology. Based on the high-quality genome sequences and previous genome sequencing projects, a number of genes that were probably subject to positive selection reside in the genome of H. molitrix, and the last common ancestors of H. molitrix and H. nobilis were identified. Some of these genes may partially explain the mechanisms of H. molitrix for surviving damage due to toxic algae. Demographic history estimation suggests that the effective population size (EPS) of the species may have constantly increased along with the uplift of the Qinghai-Tibet Plateau, started to decline when quaternary glaciation started, and further declined during the Younger Dryas Period. Moreover, the introgression from H. nobilis to H. molitrix in North America was corroborated based on the whole-genome sequencing data, and the proportion of introgressed regions was estimated to be approximately 5.8%. Based on the high-quality assembly, the possible mechanisms by which H. molitrix adapts to its endemic and invaded locations were profiled.
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http://dx.doi.org/10.1016/j.ygeno.2021.06.024DOI Listing
June 2021

Bim transfer between Bcl-2-like protein and Hsp70 underlines Bcl-2/Hsp70 crosstalk to regulate apoptosis.

Biochem Pharmacol 2021 Jun 18:114660. Epub 2021 Jun 18.

State Key Laboratory of Fine Chemicals, Zhang Dayu School of Chemistry, Dalian University of Technology, Dalian, Liaoning, China. Electronic address:

The chaperone heat shock protein 70 (Hsp70) is crucial for avoiding protein misfolding under stress, but it is also upregulated in many kinds of cancers, where its ability to buffer cellular stress prevents apoptosis. Previous research has suggested that Bim, a BH3-only member of the Bcl-2 family proteins, also serves as a cochaperone for Hsp70, which modulates the folding and stabilization of many Hsp70 oncogenic substrates in tumor cells. However, a definitive demonstration of crosstalk between Bcl-2 and Hsp70 family proteins and molecular mechanism remain unclear. Herein, we examined the effects of pan-Bcl-2 inhibitor S1, Hsp70 inhibitor S1g-6 on the K562, U937, H23, HL-60 cell lines and these inhibitors synergistically induce mitochondrial apoptosis in cancer cell lines. Moreover, we identified that Bim transfer between Bcl-2-like protein and Hsp70 underlines Bcl-2/Hsp70 crosstalk in mitochondrial apoptosis pathway. Thus, the synergy of S1 and S1g-6 to induce a panel of cancer cell lines apoptosis by inhibiting free Bim and facilitating oncogenic client AKT folding and activation. Together, our results demonstrated the combination of Bcl-2 inhibitor and Hsp70 inhibitor showed synergistic effect in cancer cells and the potential to decrease tumor regression.
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http://dx.doi.org/10.1016/j.bcp.2021.114660DOI Listing
June 2021

O-glycoforms of polymeric IgA1 in the plasma of patients with IgA nephropathy are associated with pathological phenotypes.

Nephrol Dial Transplant 2021 Jun 21. Epub 2021 Jun 21.

Renal Division, Peking University First Hospital, China.

Background: IgA1 O-glycosylation plays an important role in the pathogenesis of IgA nephropathy (IgAN). However, variations in IgA1 O-glycoforms have not been explored. We aimed to investigate the IgA1 O-glycoforms in the hinge region (HR) of polymeric IgA1 and then evaluate the association between IgA1 O-glycoforms and crescent formation in IgAN.

Methods: The discovery cohort (cohort 1) comprised 11 crescentic IgAN patients, 10 noncrescentic IgAN patients and 10 healthy controls, and the validation cohort (cohort 2) comprised 11 crescentic IgAN patients, 9 noncrescentic IgAN patients, and 9 healthy controls. A total of 143 IgAN patients with different crescent percentages (cohort 3) were also included. Polymeric IgA1 was purified from the plasma of the participants. The variation in O-glycoforms was evaluated by estimating the molecular weights of IgA1 hinge glycopeptides using reversed-phase liquid chromatography (LC) and tandem mass spectrometry under electron-transfer/higher-energy collision dissociation (EThcD) fragmentation mode.

Results: In discovery cohort (cohort 1), the numbers of GalNAc bound to one HR were lower in IgAN patients. The proportions of GalNAc3 (defined as O-glycans bound to one HR at 3 sites) and GalNAc4 were highest in crescentic IgAN patients followed by noncrescentic IgAN patients and were lowest in healthy controls (GalNAc 3: 9.92%±3.37% vs 6.65%±1.53% vs 4.05%±1.24%; P < 0.001; GalNAc4: 45.91%±4.75% vs 41.13%±2.95% vs 40.98%±2.95%; P = 0.004). The proportions of GalNAc5 and GalNAc6 were lowest in crescentic IgAN patients followed by noncrescentic IgAN patients and were highest in healthy controls (GalNAc5: 50.15%±4.27% vs 47.92%±4.09% vs 45.87%±3.79%, P = 0.028; GalNAc6: 6.58%±2.53% vs 6.04%±1.35% vs 4.65%±2.27%; P = 0.034). These results were consistent in the validation cohort (cohort 2); In another cohort with 143 patients with different crescents percentage (cohort 3), the numbers of GalNAc in polymeric IgA1 decreased with increasing percentage of crescents.

Conclusions: The numbers of GalNAc in IgA1 HRs were lower in IgAN patients, especially in crescentic IgAN patients, and may be associated with a severe IgAN phenotype.
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http://dx.doi.org/10.1093/ndt/gfab204DOI Listing
June 2021

Alkene Difunctionalization Triggered by a Stabilized Allenyl Radical: Elusively Concomitant Installation of Two Unsaturated C-C Bonds.

Angew Chem Int Ed Engl 2021 Jun 20. Epub 2021 Jun 20.

Soochow University, chemistry, 199 Ren'ai Road, 215123, Suzhou, CHINA.

Radical-mediated difunctionalization of alkenes provides a promising approach to introduce one alkenyl or alkynyl group to target compounds. However, simultaneous installation of two unsaturated C-C bonds via alkene difunctionalization remains elusive, attributable to the high instability and transient lifetimes of alkenyl and alkynyl radicals. Herein, we report the photocatalytic 1,2-alkynylalkenylation and 1,2-enynylalkenylation of alkenes for the first time, triggered by the intermolecular addition of a stabilized allenyl radical to an alkene. A portfolio of strategically designed, easily accessible dual-function reagents are applied to a radical docking-migration cascade. The protocol has broad substrate scope and efficiently increases the degree of unsaturation.
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http://dx.doi.org/10.1002/anie.202106145DOI Listing
June 2021

Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.

J Food Sci 2021 Jun 20. Epub 2021 Jun 20.

Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, China.

Ovomucoid (OVM), known as the major allergen in egg white, has gained increasing concerns in industrialized countries. Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix. We also introduced an approach to reduce the allergenicity of Gallus domesticus OVM by Maillard reaction with GOS and FOS. However, the OVM glycated by mannosan (MOS) and deglycosylated OVM exhibited higher allergenicity than native OVM. Therefore, GOS and FOS, especially GOS, could be applied in the reduction of the potential allergenicity of OVM through glycation. Furthermore, these findings may provide new insights into the development of hypoallergenic egg products. PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated. The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.
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http://dx.doi.org/10.1111/1750-3841.15791DOI Listing
June 2021

The Thermodynamic Stability, Electronic and Photocatalytic Properties of the ZnWO(100) Surface as Predicted by Screened Hybrid Density Functional Theory.

ACS Omega 2021 Jun 3;6(23):15057-15067. Epub 2021 Jun 3.

College of Physics, Sichuan University, Sichuan 610065, China.

Zinc tungstate (ZnWO) is an outstanding photocatalyst for water splitting and organic contaminant degradation under visible light irradiation. Surface termination stabilities are significant for understanding the photochemical oxidation and reactions on the ZnWO surface. Based on density functional theory, we calculated the thermodynamic stability of possible surface terminations for ZnWO(100). The surface stability phase diagrams show that the ZnO-ZnWO, WO-ZnWO, and Zn-ZnWO terminations of ZnWO(100) can be stabilized under certain thermodynamic equilibrium conditions. The electronic structures for these three possible stability surface terminations are calculated based on the Heyd-Scuseria-Ernzerhof (HSE06) hybrid functional to give dependable theoretical band gap values. It is found that the surface states of WO-ZnWO termination are in the band gap, which shows a delocalized performance. The calculated absorption coefficients of WO-ZnWO termination show stronger absorption than bulk ZnWO in the visible-light region. The band edge calculation shows that the valence band maximum and conduction band minimum of the WO-ZnWO termination can fulfill the hydrogen evolution reaction and oxygen evolution reaction requirements at the same time. Furthermore, work functions are extraordinarily distinct for various surface terminations. This result suggests that the ZnWO-based direct Z-scheme heterostructure can be controlled by obtaining the thermodynamically preferred surface termination under suitable conditions. Our results can predict ZnWO(100) surface structures and properties under the entire range of accessible environmental conditions.
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http://dx.doi.org/10.1021/acsomega.1c01214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210413PMC
June 2021

Deterioration of Avellino corneal dystrophy in a Chinese family after LASIK.

Int J Ophthalmol 2021 18;14(6):795-799. Epub 2021 Jun 18.

Eye Hospital, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.

Aim: To reveal the importance of gene screening for candidates with a family history of corneal disease or granular opacities in corneal stroma before refractive surgery.

Methods: A 37-year-old male (proband) underwent bilateral laser-assisted keratomileusis (LASIK) in 2002, with right vision decreased significantly in 2006. The proband and other 32 members of the family underwent a detailed ophthalmic examination, including vision acuity, intraocular pressure, slit-lamp photograph, fundus examination, optical coherence tomography (OCT) of cornea, and confocal microscope (IVCM) and peripheral blood was used for genomic DNA extraction. Seventeen gene exons were analyzed polymerase chain reaction amplification and direct sequencing.

Results: Slit-lamp, IVCM, and OCT images showed that a large amount of dense and confluent granular opaque were seen at the interfaces of the flap and remnant stromal bed in right and light degree in left eye. Sanger sequencing showed that there was a 371G>A mutation (CGC>CAC) in exon 4, which indicated that he harbored a heterozygote R124H mutation, identifying the diagnosis of Avellino corneal dystrophy (ACD). Among the other 32 family members, 6 of them harbored the identical mutation to that in the proband.

Conclusion: ACD will worsen and recur after LASIK. Preoperative gene-screening for mutations is important in diagnosing ACD.
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http://dx.doi.org/10.18240/ijo.2021.06.02DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165627PMC
June 2021

Novel multi-stimuli-responsive supramolecular gel based on quinoline for the fluorescence ultrasensitive detection of Feand Cu.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jun 11;261:120078. Epub 2021 Jun 11.

College of Chemical Engineering, Lanzhou University of Arts and Science, Lanzhou 730000, People's Republic of China. Electronic address:

A novel gelator molecular based on quinolone (MN) has been successfully designed and synthesized. The gelator MN could self-assemble to form a supramolecular gel (OMN), which showed obvious aggregation-induced emission (AIE) in iso-Propyl alcohol (i-PrOH). Furthermore, the supramolecular organogel OMN realized ultrasensitive detection of Fe and Cu in aqueous medium and fluorescent quenching at 427 nm. The sensing mechanism between supramolecular gel and metal ions was fully investigated via FE-SEM, FT-IR, XRD and XPS. Meanwhile, a thin film based on responsive supramolecular gel OMN was prepared, which could be used as multi-stimuli-responsive fluorescent display materials for the detection of Fe and Cu.
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http://dx.doi.org/10.1016/j.saa.2021.120078DOI Listing
June 2021

The feasibility and technical strategy of a fascia space priority approach in laparoscopic lateral lymph node dissection for advanced middle and low rectal cancer: a retrospective multicentre study.

Wideochir Inne Tech Maloinwazyjne 2021 Jun 9;16(2):312-320. Epub 2021 Apr 9.

Department of Colorectal Surgery, Tianjin Union Medical Centre, Tianjin, China.

Introduction: Laparoscopic lateral lymph node dissection (LLND) is an important treatment for patients with lateral lymph node metastasis.

Aim: To assess the technical feasibility and investigate the surgical outcomes after LLND using the fascia space priority approach for patients with advanced middle and low rectal cancer.

Material And Methods: Consecutive patients undergoing laparoscopic LLND using the fascia space priority approach from June 2017 to June 2020 were identified from 12 medical centres in mainland China. Three anatomic fascia spaces were dissected to establish the boundaries of the LLND, and the obturator and internal iliac lymph nodes were excised in an en bloc manner. Retrospective clinical data including patient characteristics, surgical details, and pathology were analysed.

Results: A total of 112 patients were identified. All surgeries were completed laparoscopically with no conversions. The mean operation time was 343.6 ±103.8 min for the entire procedure. The median blood loss was 100 ml (range: 100-700 ml). The median lymph node yield was 6 (range: 1-41), and lymph nodes were positive in 39.3% (44/112) of the patients. Sixteen (14.3%) patients had Clavien-Dindo I-II complications, no Clavien-Dindo III-IV complications were identified. The incidence of complications between the bilateral dissection group and the unilateral dissection group was not statistically different (p = 0.19). The complication rate between the "nCRT" group and the "no nCRT" group was not significantly different (p = 0.62) either. There were no perioperative deaths.

Conclusions: Laparoscopic LLND using the fascia space priority approach is feasible and safe for patients with lateral lymph node metastasis.
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http://dx.doi.org/10.5114/wiitm.2021.105143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193747PMC
June 2021

Water Microdroplets Allow Spontaneously Abiotic Production of Peptides.

J Phys Chem Lett 2021 Jun 16:5774-5780. Epub 2021 Jun 16.

School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, China.

The chemistry of abiotic synthesis of peptides in the context of their prebiotic origins is a continuing challenge that arises from thermodynamic and kinetic constraints in aqueous media. Here we reported a strategy of microdroplets' mass spectrometry for peptide bonds formed from pure amino acids or a mixture in the presence of phosphoric acids in aqueous microdroplets. In contrast to bulk experiments, the condensation reactions proceed spontaneously under ambient conditions. The microdroplet gave a negative free-energy change (Δ ∼ -1.1 kcal/mol), and product yields of ∼75% were obtained at the scale of a few milliseconds. Experiments in which nebulization gas pressure and external charge were varied established dependence of peptide production on the droplet size that has a high surface-to-volume ratio. It is concluded that the condensation reactions occurred at or near the air-water interfaces of microdroplets. This aqueous microdroplets approach also provides a route for chemistry synthesis in the prebiotic era.
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http://dx.doi.org/10.1021/acs.jpclett.1c01083DOI Listing
June 2021

Potential dissemination mechanism of the tetC gene in Aeromonas media from the aerobic biofilm reactor under oxytetracycline stresses.

J Environ Sci (China) 2021 Jul 15;105:90-99. Epub 2021 Jan 15.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address:

The tetC gene has been found to be one of the most widely distributed tetracycline resistance (tet) genes in various environmental niches, but the detailed dissemination mechanisms are still largely unknown. In the present study, 11 tetC-containing Aeromonas media strains were isolated from an aerobic biofilm reactor under oxytetracycline stresses, and the genome of one strain was sequenced using the PacBio RSII sequencing approach to reveal the genetic environment of tetC. The tetC gene was carried by an IS26 composite transposon, named Tn6434. The tetC-carrying Tn6434 structure was detected in all of the A. media strains either in a novel plasmid pAeme2 (n=9) or other DNA molecules (n=2) by PCR screening. The NCBI database searching result shows that this structure was also present in the plasmids or chromosomes of other 13 genera, indicating the transferability of Tn6434. Inverse PCR and sequencing confirmed that Tn6434 can form a circular intermediate and is able to incorporate into a preexisting IS26 element, suggesting that Tn6434 might be responsible for the dissemination of tetC between different DNA molecules. This study will be helpful in uncovering the spread mechanism of tet genes in water environments.
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http://dx.doi.org/10.1016/j.jes.2020.12.038DOI Listing
July 2021

Quantitative analysis of retinal vessel density and thickness changes in diabetes mellitus evaluated using optical coherence tomography angiography: a cross-sectional study.

BMC Ophthalmol 2021 Jun 15;21(1):259. Epub 2021 Jun 15.

Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Background: Diabetic retinopathy is the most common microvascular complication of diabetes; however, early changes in retinal microvessels are difficult to detect clinically, and a patient's vision may have begun to deteriorate by the time a problem is identified. Optical coherence tomography angiography (OCTA) is an innovative tool for observing capillaries in vivo. The aim of this study was to analyze retinal vessel density and thickness changes in patients with diabetes.

Methods: This was a retrospective, observational cross-sectional study. Between August 2018 and February 2019, we collected OCTA data from healthy participants and diabetics from the First Affiliated Hospital of Harbin Medical University. Analyzed their retinal vessel density and thickness changes.

Results: A total of 97 diabetic patients with diabetes at different severity stages of diabetic retinopathy and 85 controls were involved in the experiment. Diabetic patients exhibited significantly lower retinal VD (particularly in the deep vascular complexes), thickening of the neurosensory retina, and thinning of the retinal pigment epithelium compared with controls. In the control group, nondiabetic retinopathy group and mild diabetic retinopathy group, superficial VD was significantly correlated with retinal thickness (r = 0.3886, P < 0.0001; r = 0.3276, P = 0.0019; r = 0.4614, P = 0.0024, respectively).

Conclusions: Patients with diabetes exhibit ischemia of the retinal capillaries and morphologic changes in vivo prior to vision loss. Therefore, OCTA may be useful as a quantitative method for the early detection of diabetic retinopathy.
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http://dx.doi.org/10.1186/s12886-021-01988-2DOI Listing
June 2021

Human Induced pluripotent stem cells-derived corneal endothelial-like cells promote corneal transparency in a rabbit model of bullous keratopathy.

Stem Cells Dev 2021 Jun 15. Epub 2021 Jun 15.

First Affiliated Hospital of Harbin Medical University, 74559, Harbin, Heilongjiang, China;

The corneal endothelium(CE) is vital for the cornea to maintain its transparency. However, CE dysfunction occurs due to aging, intraocular surgery, trauma, dystrophy, etc. Corneal transplantation is the only method to clinically treat CE dysfunction; however, this treatment strategy faces the disadvantages of a global cornea shortage, graft failure, and severe side effects. There is a recognized need for a substitute for the CE. Stem cells are becoming increasingly common for the treatment of human diseases. In fact, several studies have documented the induction of corneal endothelial-like cells (CECs) from stem cells, but an ideal procedure has not yet been established. Thus, this study aimed to explore a more efficient and robust differentiation method. We used a modified approach to differentiate induced pluripotent stem cells (iPSCs) into CECs. After the identification of differentiated CECs, the CECs were injected into the anterior chambers of the eyes of a rabbit model of bullous keratopathy. The rabbits were maintained in the eye-down position to ensure that the cells attached to the cornea. The results showed that corneal edema was alleviated in the rabbits injected with CECs compared with that in the rabbits belonging to the control group. This study extends the ability to differentiate iPSCs into CECs and provides a potential strategy for the treatment of reduced visual acuity caused by CE deficiency in the future.
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http://dx.doi.org/10.1089/scd.2020.0205DOI Listing
June 2021

The downregulation of NCXs is positively correlated with the prognosis of stage II-IV colon cancer.

World J Surg Oncol 2021 Jun 14;19(1):177. Epub 2021 Jun 14.

Colorectal Tumor Surgery Ward, Department of General Surgery, Shengjing Hospital of China Medical University, No. 36, San Hao Street, Shenyang, Liaoning, People's Republic of China.

Purpose: Colon cancer (CC) is a very common gastrointestinal tumor that is prone to invasion and metastasis in the late stage. This study aims to observe the expression of Na/Ca exchangers (NCXs) and analyze the correlation between NCXs and the prognosis of CC.

Methods: Specimens of 111 stage II-IV CC patients were collected. We used western blotting, qPCR, and immunohistochemical staining to observe the distributions and expression levels of NCX isoforms (NCX1, NCX2, and NCX3) in CC and distal normal tissues. Cox proportional hazards models were used to assess prognostic factors for patients.

Results: The expression of NCXs in most tumor specimens was lower than that in normal tissues. The NCX expression levels in tumor tissues from the primary tumor, local lymph node metastasis sites, and distant liver metastasis sites were increasingly significantly lower than those in normal tissues. The results of the Kaplan-Meier survival curves showed that the downregulation of any NCX isoform was closely related to the worse prognosis of advanced CC.

Conclusion: NCXs can be used as independent prognostic factors for CC. Our research results are expected to provide new targets for the treatment of CC.
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http://dx.doi.org/10.1186/s12957-021-02284-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204472PMC
June 2021

Photodynamic Therapy for Recurrent Early Central Lung Cancer of Great Longitudinal Extent Following Two Surgical Lung Resections: A Case Report.

Photodiagnosis Photodyn Ther 2021 Jun 11:102400. Epub 2021 Jun 11.

Department of Pulmonary and Critical Care Medicine, Peking University First Hospital, Beijing, Beijing 100034, China. Electronic address:

Photodynamic therapy (PDT) is an endobronchial treatment requiring a photosensitizer activated by a specific wavelength light to kill tumor cells, which is effective in treating early central lung cancer (ECLC) especially for lesions <1.0 cm in length. We present a patient with history of two lung resections for squamous cell carcinoma, who had unresectable ECLC lesions (4.0 - 5.0 cm in length) treated by PDTs successfully without other modalities, such as radiotherapy or chemotherapy. After sequential PDTs, the patient achieved complete response for 2 months and partial response for 16 months, with greatly improved quality of life, despite mild skin photosensitization and acute exacerbation of chronic obstructive pulmonary disease. There was no evidence of metastasis during standard evaluation. As it was less-invasive and highly targeted, PDT might be a relatively safe and effective alternative therapy for ECLC lesions unsuitable for surgery, even lesions longer than 1.0 cm.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102400DOI Listing
June 2021

Autophagy-Mediated Clearance of Free Genomic DNA in the Cytoplasm Protects the Growth and Survival of Cancer Cells.

Front Oncol 2021 26;11:667920. Epub 2021 May 26.

Department of Pathology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.

The cGAS (GMP-AMP synthase)-mediated senescence-associated secretory phenotype (SASP) and DNA-induced autophagy (DNA autophagy) have been extensively investigated in recent years. However, cGAS-mediated autophagy has not been elucidated in cancer cells. The described investigation revealed that active DNA autophagy but not SASP activity could be detected in the BT-549 breast cancer cell line with high micronucleus (MN) formation. DNA autophagy was identified as selective autophagy of free genomic DNA in the cytoplasm but not nucleophagy. The process of DNA autophagy in the cytosol could be initiate by cGAS and usually cooperates with SQSTM1-mediated autophagy of ubiquitinated histones. Cytoplasmic DNA, together with nuclear proteins such as histones, could be derived from DNA replication-induced nuclear damage and MN collapse. The inhibition of autophagy through chemical inhibitors as well as the genomic silencing of cGAS or SQSTM1 could suppress the growth and survival of cancer cells, and induced DNA damage could increase the sensitivity to these inhibitors. Furthermore, expanded observations of several other kinds of human cancer cells indicated that high relative DNA autophagy or enhancement of DNA damage could also increase or sensitize these cells to inhibition of DNA autophagy.
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http://dx.doi.org/10.3389/fonc.2021.667920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189927PMC
May 2021

An interparticle relatively motional DNA walker and its sensing application.

Chem Sci 2020 Jun 30;11(28):7415-7423. Epub 2020 Jun 30.

School of Chemistry and Chemical Engineering, Shandong University 250100 Jinan P. R. China

DNA molecular machines are DNA self-assemblies that perform quasi-mechanical movement at the micro-nano scale, and have attracted increasing attention in the fields of biosensing, drug delivery and biocomputing. Herein, we report the concept and operation of an interparticle relatively motional DNA walker. The walker is composed of walking particles (WPs) and track particles (TPs). The WPs and TPs are obtained by respective functionalization of locked walking strands containing DNAzyme sequences and fluorophore-labelled track strands containing substrate sequences onto gold nanoparticles (AuNPs). Triggered by the target that specifically unlocks the walking strand, the liberated walking strands cooperatively hybridize with the track strands. The track strand gets cleaved by the DNAzyme, accompanied by the fluorophore release. The adjacent walking strand on the WP subsequently hybridizes to the next track strand, inducing the relative motion of the WP around the TP. After walking along the surface of one TP, the WP can continue to interact with another TP. As a result of the improved moving freedom and area, the interparticle motional mode induces high continuity and achieves large signal accumulation. Taking Zika virus RNA fragments (ZIKV-RNA) as a model target, the DNA walker shows a high sensitivity with a detection limit of 118 pM, and can reliably detect the target in biological fluids due to the stability of its components. The constructed DNA walker provides a new type of free and robust motion mode between particles and holds potential in clinical diagnosis.
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http://dx.doi.org/10.1039/d0sc00109kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159414PMC
June 2020

A Phase I, Randomized, Single-Dose Study to Evaluate the Biosimilarity of HOT-3010 to Adalimumab Among Healthy Chinese Male Subjects.

Front Pharmacol 2021 17;12:646171. Epub 2021 Mar 17.

Phase I Clinical Research Center, The First Hospital of Jilin University, Jilin, China.

This study explored the bioequivalence of a proposed biosimilar HOT-3010 vs. its reference product (adalimumab) among healthy Chinese male subjects. The study also investigated the tolerance, immunogenicity, and pharmacokinetics (PK). A randomized, double-blind, two-arm, parallel study was performed to examine the bioequivalence of HOT-3010 (40 mg) with that of adalimumab (Humira, AbbVie) as a reference drug. The study subjects were followed up for 71 days. PK properties exhibited by HOT-3010 ( = 66) and adalimumab ( = 68) groups were similar. The 90% confidence intervals of the ratios for , AUC , and AUC were observed to be in the range 80-125% on comparing the two groups. For anti-drug antibodies (ADA), the number of subjects found to be positive in the HOT-3010 group and adalimumab group were 29 (43.94%) and 32 (47.06%), whereas 27 (40.91%) and 27 (39.71%) subjects were found to be positive for NAb, respectively. Treatment-related treatment-emergent adverse events (TEAEs) were recorded in 32 subjects each in both the groups, respectively. The PK characteristics and immunogenicity exhibited by HOT-3010 were similar to that of the reference product, adalimumab. The safety profiles were similar in both the treatment groups with mild-moderate adverse effects.
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http://dx.doi.org/10.3389/fphar.2021.646171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190825PMC
March 2021

Brain structural alterations in MDD patients with gastrointestinal symptoms: Evidence from the REST-meta-MDD project.

Prog Neuropsychopharmacol Biol Psychiatry 2021 Jun 11;111:110386. Epub 2021 Jun 11.

Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Objective: While gastrointestinal (GI) symptoms are very common in patients with major depressive disorder (MDD), few studies have investigated the neural basis behind these symptoms. In this study, we sought to elucidate the neural basis of GI symptoms in MDD patients by analyzing the changes in regional gray matter volume (GMV) and gray matter density (GMD) in brain structure.

Method: Subjects were recruited from 13 clinical centers and categorized into three groups, each of which is based on the presence or absence of GI symptoms: the GI symptoms group (MDD patients with at least one GI symptom), the non-GI symptoms group (MDD patients without any GI symptoms), and the healthy control group (HCs). Structural magnetic resonance images (MRI) were collected of 335 patients in the GI symptoms group, 149 patients in the non-GI symptoms group, and 446 patients in the healthy control group. The 17-item Hamilton Depression Rating Scale (HAMD-17) was administered to all patients. Correlation analysis and logistic regression analysis were used to determine if there was a correlation between the altered brain regions and the clinical symptoms.

Results: There were significantly higher HAMD-17 scores in the GI symptoms group than that of the non-GI symptoms group (P < 0.001). Both GMV and GMD were significant different among the three groups for the bilateral superior temporal gyrus, bilateral middle temporal gyrus, left lingual gyrus, bilateral caudate nucleus, right Fusiform gyrus and bilateral Thalamus (GRF correction, cluster-P < 0.01, voxel-P < 0.001). Compared to the HC group, the GI symptoms group demonstrated increased GMV and GMD in the bilateral superior temporal gyrus, and the non-GI symptoms group demonstrated an increased GMV and GMD in the right superior temporal gyrus, right fusiform gyrus and decreased GMV in the right Caudate nucleus (GRF correction, cluster-P < 0.01, voxel-P < 0.001). Compared to the non-GI symptoms group, the GI symptoms group demonstrated significantly increased GMV and GMD in the bilateral thalamus, as well as decreased GMV in the bilateral superior temporal gyrus and bilateral insula lobe (GRF correction, cluster-P < 0.01, voxel-P < 0.001). While these changed brain areas had significantly association with GI symptoms (P < 0.001), they were not correlated with depressive symptoms (P > 0.05). Risk factors for gastrointestinal symptoms in MDD patients (p < 0.05) included age, increased GMD in the right thalamus, and decreased GMV in the bilateral superior temporal gyrus and left Insula lobe.

Conclusion: MDD patients with GI symptoms have more severe depressive symptoms. MDD patients with GI symptoms exhibited larger GMV and GMD in the bilateral thalamus, and smaller GMV in the bilateral superior temporal gyrus and bilateral insula lobe that were correlated with GI symptoms, and some of them and age may contribute to the presence of GI symptoms in MDD patients.
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http://dx.doi.org/10.1016/j.pnpbp.2021.110386DOI Listing
June 2021

Aberrant Bcl-x splicing in cancer: from molecular mechanism to therapeutic modulation.

J Exp Clin Cancer Res 2021 Jun 12;40(1):194. Epub 2021 Jun 12.

Department of Heavy Ion Radiation Medicine, Bio-Medical Research Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, China.

Bcl-x pre-mRNA splicing serves as a typical example to study the impact of alternative splicing in the modulation of cell death. Dysregulation of Bcl-x apoptotic isoforms caused by precarious equilibrium splicing is implicated in genesis and development of multiple human diseases, especially cancers. Exploring the mechanism of Bcl-x splicing and regulation has provided insight into the development of drugs that could contribute to sensitivity of cancer cells to death. On this basis, we review the multiple splicing patterns and structural characteristics of Bcl-x. Additionally, we outline the cis-regulatory elements, trans-acting factors as well as epigenetic modifications involved in the splicing regulation of Bcl-x. Furthermore, this review highlights aberrant splicing of Bcl-x involved in apoptosis evade, autophagy, metastasis, and therapy resistance of various cancer cells. Last, emphasis is given to the clinical role of targeting Bcl-x splicing correction in human cancer based on the splice-switching oligonucleotides, small molecular modulators and BH3 mimetics. Thus, it is highlighting significance of aberrant splicing isoforms of Bcl-x as targets for cancer therapy.
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http://dx.doi.org/10.1186/s13046-021-02001-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196531PMC
June 2021

PD-L1 Expression in Metaplastic Breast Carcinoma Using the PD-L1 SP142 Assay and Concordance Among PD-L1 Immunohistochemical Assays.

Am J Surg Pathol 2021 Jun 10. Epub 2021 Jun 10.

Departments of Pathology Medicine Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY.

Immunotherapy for the treatment of programmed death-ligand 1 (PD-L1) positive locally advanced or metastatic triple negative breast cancer may benefit patients with metaplastic breast cancer (MpBC). Previous study of PD-L1 in MpBC scored tumor cells (TCs), different from Food and Drug Administration-approved scoring methods. We sought to define PD-L1 expression in MpBCs and to evaluate concordance of 3 PD-L1 assays. Primary, treatment naive MpBC treated at our Center from 1998 to 2019 were identified. PD-L1 expression was assessed using SP142, E1L3n, and 73-10. We evaluated PD-L1 expression on tumor infiltrating immune cells (IC) and also in TCs. For each assay, we scored PD-L1 expression using ≥1% IC expression according to the IMpassion130 trial criteria and using combined positive score (CPS) ≥10 according to the KEYNOTE-355 trial cutoff. A total of 42 MpBCs were identified. Most MpBC had PD-L1 positivity in ≥1% IC with all 3 assays (95%, 95%, 86%) in contrast to a maximum 71% with a CPS ≥10. PD-L1 IC expression was comparable between the SP142 and 73-10 assays and was lowest with E1L3n. PD-L1 TC expression was lowest using SP142. The overall concordance for IC scoring was 88% while 62% had concordant CPS. For each assay, the results of the 2 scoring algorithms were not interchangeable. The SP142 assay showed distinct expression patterns between IC (granular, dot-like) and TC (membranous) while 73-10 and E1L3n showed membranous and/or cytoplasmic expression in both IC and TC. Most MpBC in our cohort were positive for PD-L1 indicating eligibility for anti-PD-L1/programmed death-1 immunotherapy.
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http://dx.doi.org/10.1097/PAS.0000000000001760DOI Listing
June 2021

Monolayered H-Si-P semiconductors: structural stability, electronic structure, optical properties, and prospects for photocatalytic water splitting.

Phys Chem Chem Phys 2021 Jun 11. Epub 2021 Jun 11.

College of Physical Science and Technology, Sichuan University, Chengdu, 610065, China.

Group IV and V monolayers are promising state-of-the-art two-dimensional (2D) materials owing to their high carrier mobility, tunable bandgaps, and optical linear dichroism along with outstanding electronic and thermoelectric properties. Furthermore, recent studies revealed the stability of free-standing 2D monolayers by hydrogenation. Inspired by this, we systematically predicted and investigated the structure and properties of various hydrogen-saturated silicon phosphide (H-Si-P) monolayers based on first-principles calculations. According to the results, H-Si-P monolayers belong to indirect bandgap semiconductors with a highly stable structure. Their bandgaps and band edge positions assessed using accurate hybrid functional are shown to be effectively adjusted by applying a biaxial strain. Furthermore, the absorption spectra of these monolayers, simulated in the context of time-dependent density functional theory, exhibit their excellent potential for solar energy conversion and visible-light-driven photocatalytic water splitting. In this respect, this work provides valuable guidance for finding more 2D semiconductors and nanostructures for nanoelectronics and optoelectronic applications, as well as for photocatalytic water splitting.
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http://dx.doi.org/10.1039/d1cp01219cDOI Listing
June 2021

Long non-coding RNA IGF2-AS promotes trophoblast cell proliferation, migration, and invasion by regulating miR-520g/N-cadherin axis.

J Obstet Gynaecol Res 2021 Jun 9. Epub 2021 Jun 9.

Department of Obstetrics and Gynecology, Jingmen No.1 People's Hospital, Jingmen, China.

Background: Recurrent miscarriage (RM) is a distressing reproductive issue worldwide. Dysfunction of trophoblasts can trigger numerous unfavorable pregnant outcomes such as RM, stillbirth, and fetal malformation.

Methods: In this text, the roles and molecular basis of long non-coding RNA insulin growth factor 2 antisense (IGF2-AS) in the development of trophoblast cells were further investigated. IGF2-AS, microRNA-520g (miR-520g), and N-cadherin levels were measured by RT-qPCR assay. Cell viability, the number of colonies, cell apoptosis, migration, and invasion were measured by CCK-8 assay, colony formation assay, flow cytometry, transwell migration, and invasion assays, respectively. The relative proteins expression was detected by western blot.

Results: The interaction between miR-520g and IGF2-AS or N-cadherin was tested by bioinformatics prediction analysis, and confirmed by dual-luciferase reporter assay, RNA pull-down assay, and RNA immunoprecipitation (RIP) assay. Our data revealed that IGF2-AS and N-cadherin levels were notably decreased, and miR-520g was strikingly increased in the placentas from RM patients. IGF2-AS overexpression promoted cell proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), and hampered cell apoptosis in trophoblast cells, while IGF2-AS deletion exhibited opposite results. Moreover, miR-520g was a target gene of IGF2-AS and negatively regulated by IGF2-AS. MiR-520g inhibitor enhanced the proliferation, migration, and invasion capability of trophoblast cells, suppressed cell apoptosis, and promoted the EMT process. Moreover, the effects of IGF2-AS overexpression on trophoblast cells were reversed by miR-520g upregulation.

Conclusions: These findings indicated that IGF2-AS facilitated trophoblast cell proliferation, migration, invasion, EMT, and suppressed cell apoptosis by regulating miR-520g/N-cadherin axis, providing potential biomarkers for RM.
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http://dx.doi.org/10.1111/jog.14886DOI Listing
June 2021

DHA ameliorates MeHg‑induced PC12 cell apoptosis by inhibiting the ROS/JNK signaling pathway.

Mol Med Rep 2021 Aug 10;24(2). Epub 2021 Jun 10.

Ministry of Education‑Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China.

Recent studies have reported that methylmercury (MeHg) induces neuronal apoptosis, which is accompanied by abnormal neurological development. Despite the important role of docosahexaenoic acid (DHA) in maintaining the structure and function of the brain, as well as improving neuronal apoptosis induced by MeHg, the exact mechanism remains unknown. The present study hypothesized that the reactive oxygen species (ROS)‑mediated JNK signaling pathway may be associated with the protective effect of DHA against MeHg‑induced PC12 cell apoptosis. Cell Counting Kit‑8, TUNEL staining, flow cytometry, ROS detection, PCR and western blot analysis were performed. The results demonstrated that MeHg inhibited the activity of PC12 cells, causing oxidative damage and promoting apoptosis; however, DHA significantly attenuated this effect. Mechanistic studies revealed that MeHg increased intracellular ROS levels and JNK protein phosphorylation, and decreased the expression levels of the anti‑apoptotic protein Bcl‑2, whereas DHA reduced ROS levels and JNK phosphorylation, and increased Bcl‑2 expression. In addition, the ROS inhibitor N‑acetyl‑l‑cysteine (NAC) was used to verify the experimental results. After pretreatment with NAC, expression levels of Bcl‑2, Bax, phosphorylated‑JNK and JNK were assessed. Bcl‑2 protein expression was increased and the Bcl‑2/Bax ratio was increased. Moreover, the high expression levels of phosphorylated‑JNK induced by MeHg were significantly decreased. Based on the aforementioned results, the present study indicated that the effects of DHA against MeHg‑induced PC12 cell apoptosis may be mediated via the ROS/JNK signaling pathway.
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http://dx.doi.org/10.3892/mmr.2021.12197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188641PMC
August 2021

Targeting Endothelial Cell-Specific Molecule 1 Protein in Cancer: A Promising Therapeutic Approach.

Front Oncol 2021 24;11:687120. Epub 2021 May 24.

Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Despite the dramatic advances in cancer research in the past few years, effective therapeutic strategies are urgently needed. Endothelial cell-specific molecule 1 (ESM-1), a soluble dermatan sulfate proteoglycan, also known as endocan, serves as a diagnostic and prognostic indicator due to its aberrant expression under pathological conditions, including cancer, sepsis, kidney diseases, and cardiovascular disease. Significantly, ESM-1 can promote cancer progression and metastasis through the regulation of tumor cell proliferation, migration, invasion, and drug resistant. In addition, ESM-1 is involved in the tumor microenvironment, containing inflammation, angiogenesis, and lymph angiogenesis. This article reviews the molecular and biological characteristics of ESM-1 in cancer, the underlying mechanisms, the currently clinical and pre-clinical applications, and potential therapeutic strategies. Herein, we propose that ESM-1 is a new therapeutic target for cancer therapy.
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http://dx.doi.org/10.3389/fonc.2021.687120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181400PMC
May 2021

Multimodal host-guest complexation for efficient and stable perovskite photovoltaics.

Nat Commun 2021 Jun 7;12(1):3383. Epub 2021 Jun 7.

Laboratory of Photonics and Interfaces, Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Formamidinium lead iodide perovskites are promising light-harvesting materials, yet stabilizing them under operating conditions without compromising optimal optoelectronic properties remains challenging. We report a multimodal host-guest complexation strategy to overcome this challenge using a crown ether, dibenzo-21-crown-7, which acts as a vehicle that assembles at the interface and delivers Cs ions into the interior while modulating the material. This provides a local gradient of doping at the nanoscale that assists in photoinduced charge separation while passivating surface and bulk defects, stabilizing the perovskite phase through a synergistic effect of the host, guest, and host-guest complex. The resulting solar cells show power conversion efficiencies exceeding 24% and enhanced operational stability, maintaining over 95% of their performance without encapsulation for 500 h under continuous operation. Moreover, the host contributes to binding lead ions, reducing their environmental impact. This supramolecular strategy illustrates the broad implications of host-guest chemistry in photovoltaics.
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http://dx.doi.org/10.1038/s41467-021-23566-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185086PMC
June 2021

Survivin promotes rheumatoid arthritis fibroblast-like synoviocyte cell proliferation, and the expression of angiogenesis-related proteins by activating the NOTCH pathway.

Int J Rheum Dis 2021 Jun 7. Epub 2021 Jun 7.

Medical School, Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, China.

Background: Survivin have been shown to play a crucial role in rheumatoid arthritis (RA); however, the regulatory mechanism of survivin in RA has not been fully elucidated. This study aims to investigate the effect of survivin on the proliferation and apoptosis of human RA fibroblast-like synoviocyte (RA-HFLS) cells in RA and its underlying mechanism through the NOTCH pathway.

Methods: The RA synovial tissues of 65 RA patients with partial resection of synovium of knee joint by arthroscopy were collected. The expression of survivin in synovial tissue was detected by immunohistochemistry, and the correlation of survivin expression and clinical-pathological parameters of patients was analyzed. In vitro, the proliferation of HFLS and RA-HFLS were detected by MTT; the apoptosis of HFLS and RA-HFLS were detected by flow cytometry; the expression of survivin proteins, key protein factors (Notch1, Jagged1, Hes1) in the Notch pathway, and angiogenesis-related proteins (vascular endothelial growth factor receptor 1 [VEGFR1], Ang1, Ang2) were determined by western blot.

Results: We found that survivin was highly expressed in RA synovial tissues and RA-HFLS cells, and was positively correlated with erythrocyte sedimentation rate, cyclic citrullinated peptide, C-reactive protein, Disease Activity Score of 28 joints and other pathological indexes. Knockdown survivin induces RA-HFLS cell apoptosis, suppresses proliferation and the expression of VEGFR1, Ang1, Ang2. In addition, blocking Notch pathway using FLI-06 significantly down-regulated survivin expression. When survivin is up-regulated, it promotes RA-HFLS cell proliferation, the expression of VEGFR1, Ang1, Ang2 and suppresses apoptosis by activating the NOTCH.

Conclusion: This study confirmed that survivin promotes RA-HFLS cell proliferation, the expression of angiogenesis-related proteins and suppresses apoptosis by activating the NOTCH pathway.
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http://dx.doi.org/10.1111/1756-185X.14150DOI Listing
June 2021

Durable Response to Immunotherapy With Antiangiogenic Drug in Large-Cell Lung Carcinoma With Multiple Fulminant Postoperative Metastases: A Case Report.

Front Oncol 2021 20;11:633446. Epub 2021 May 20.

Department of Thoracic Surgery, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Immunotherapy alone or chemo-immunotherapy has recently been recommended for treating advanced lung carcinoma in patients without driver mutations. However, the efficacy of immunotherapy and molecular mechanism in large-cell lung cancer (LCLC) remains unclear. Here, we reported a rare case of multiple fulminant postoperative body and mouth metastases in LCLC treating with combination immunotherapy. Initially, the patient was diagnosed as early stage LCLC and underwent a radical resection of the right lower lobe. Just one month later, multiple fulminant body and mouth lesions appeared in the right upper arm, right elbow, right waist, and tongue root. Meanwhile, serum neuron specific enolase (NSE) concentration dramatically increased from 12.12 to 30.14 ng/ml. Immumohistochemistry findings demonstrated moderate PD-L1 expressions with tumor proportion score (TPS), while next-generation sequencing indicated moderate tumor mutational burden (TMB) levels and gene mutations in L1230P and L194R of both foci. Besides, loss of heterozygosity (LOH) at human leukocyte antigen (HLA) class I (HLA-A*02:03, HLA-B*55:02 and HLA-C*12:03) were detected in the right upper arm metastasis, which may facilitate malignant postoperative metastases in this case. Notably, this patient received combination therapy with anti-PD-1 antibody sintilimab plus anlotinib, and achieved a partial response for at least 12 months. Using an integrated computational method, the mutant peptide TEIPENDIPL derived from PBRM1 L1230P was predicted to be a specific neoantigen and could still be presented by HLA-B*40:01. This case suggests that immunotherapy plus antiangiogenic drug may provide an alternative therapeutic option for advanced LCLC patients without common gene mutations.
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http://dx.doi.org/10.3389/fonc.2021.633446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173040PMC
May 2021

Extension of the Simmons-Smith reaction to metal-carbynes: efficient synthesis of metallacyclopropenes with σ-aromaticity.

Chem Sci 2020 Sep 3;11(37):10159-10166. Epub 2020 Sep 3.

State Key Laboratory of Physical Chemistry of Solid Surfaces and Collaborative Innovation Center of Chemistry for Energy Materials (iChEM), College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China

The Simmons-Smith reaction offers a direct route for conversion of an alkene into a cyclopropane with a zinc carbenoid as the active intermediate. Zinc carbenoids, however, have never delivered a methylene unit to substrates with metal-carbon multiple bonds. Herein, we describe this type of reaction and the construction of three-membered rings has now been applied in organometallic systems by combining classical zinc carbenoid reagents with a range of structurally and electronically diverse metal carbynes. A variety of metallacyclopropene derivatives prepared in this way represent rare examples with σ-aromaticity in an unsaturated three-membered ring. The structures of such products are supported by experimental observations and theoretical calculations.
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http://dx.doi.org/10.1039/d0sc03215hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162146PMC
September 2020

Clinical Analysis of Bilateral Thalamic Infarction Caused by Percheron Artery Occlusion.

Neuropsychiatr Dis Treat 2021 28;17:1707-1712. Epub 2021 May 28.

Department of Neurology, The First Hospital of Yulin, Yulin, People's Republic of China.

The Percheron artery (artery of Percheron, AOP) is a rare variant vessel. Its acute occlusion can cause a bilateral symmetrical thalamic stroke; typical symptoms of bilateral paramedian thalamic infarcts due to occlusion of AOP are vertical gaze palsy, memory impairment, confusion, drowsiness, hypersomnolence, or coma. We present the MR imaging findings in two cases with cerebral infarction caused by Percheron artery occlusion. Due to the difficulty in the diagnosis of acute Percheron arterial infarction, early conservative treatment is used. The prognosis of the disease is poor, with few patients completely rehabilitating. Therefore, clinicians must understand the characteristics of the disease, provide early diagnosis and administer timely and effective treatment to reduce the patient's disability rate and fatality rate and therefore improve the quality of life of patients. The patient's prognosis has extraordinary significance.
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http://dx.doi.org/10.2147/NDT.S296685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169551PMC
May 2021