Publications by authors named "Hong Yu"

1,744 Publications

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ICD Coding from Clinical Text Using Multi-Filter Residual Convolutional Neural Network.

Authors:
Fei Li Hong Yu

Proc Conf AAAI Artif Intell 2020 Feb 3;34(5):8180-8187. Epub 2020 Apr 3.

Department of Computer Science, University of Massachusetts Lowell, Lowell, MA, United States.

Automated ICD coding, which assigns the International Classification of Disease codes to patient visits, has attracted much research attention since it can save time and labor for billing. The previous state-of-the-art model utilized one convolutional layer to build document representations for predicting ICD codes. However, the lengths and grammar of text fragments, which are closely related to ICD coding, vary a lot in different documents. Therefore, a flat and fixed-length convolutional architecture may not be capable of learning good document representations. In this paper, we proposed a -Filter idual onvolutional eural etwork (MultiResCNN) for ICD coding. The innovations of our model are two-folds: it utilizes a multi-filter convolutional layer to capture various text patterns with different lengths and a residual convolutional layer to enlarge the receptive field. We evaluated the effectiveness of our model on the widely-used MIMIC dataset. On the full code set of MIMIC-III, our model outperformed the state-of-the-art model in 4 out of 6 evaluation metrics. On the top-50 code set of MIMIC-III and the full code set of MIMIC-II, our model outperformed all the existing and state-of-the-art models in all evaluation metrics. The code is available at https://github.com/foxlf823/Multi-Filter-Residual-Convolutional-Neural-Network.
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http://dx.doi.org/10.1609/aaai.v34i05.6331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315310PMC
February 2020

RNA demethylation increases the yield and biomass of rice and potato plants in field trials.

Nat Biotechnol 2021 Jul 22. Epub 2021 Jul 22.

Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

RNA N-methyladenosine (mA) modifications are essential in plants. Here, we show that transgenic expression of the human RNA demethylase FTO in rice caused a more than threefold increase in grain yield under greenhouse conditions. In field trials, transgenic expression of FTO in rice and potato caused ~50% increases in yield and biomass. We demonstrate that the presence of FTO stimulates root meristem cell proliferation and tiller bud formation and promotes photosynthetic efficiency and drought tolerance but has no effect on mature cell size, shoot meristem cell proliferation, root diameter, plant height or ploidy. FTO mediates substantial mA demethylation (around 7% of demethylation in poly(A) RNA and around 35% decrease of mA in non-ribosomal nuclear RNA) in plant RNA, inducing chromatin openness and transcriptional activation. Therefore, modulation of plant RNA mA methylation is a promising strategy to dramatically improve plant growth and crop yield.
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http://dx.doi.org/10.1038/s41587-021-00982-9DOI Listing
July 2021

Exploration of transmission chain and prevention of the recurrence of coronavirus disease 2019 in Heilongjiang Province due to in-hospital transmission.

World J Clin Cases 2021 Jul;9(20):5420-5426

Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin 150001, Heilongjiang Province, China.

The coronavirus disease 2019 (COVID-19) epidemic is a major public health emergency characterized by fast spread, a wide range of infections, and enormous control difficulty. Since the end of December 2019, Wuhan has become the first core infection area of China's COVID-19 outbreak. Since March 2020, the domestic worst-hit areas have moved to the Heilongjiang Province due to the increased number of imported COVID-19 cases. Herein, we reported the major COVID-19 outbreak, which caused a rebound of the epidemic in Harbin, China. After the rebound, different levels of causes for the recurrence of COVID-19, including city-level, hospital-level, and medical staff-level cause, were investigated. Meanwhile, corresponding countermeasures to prevent the recurrence of the epidemic were also carried out on the city level, hospital level, and medical staff level, which eventually showed the effect of infection control function in a pandemic. In this study, we described the complete transmission chain, analyzed the causes of the outbreak, and proposed corresponding countermeasures from our practical clinical experience, which can be used as a valuable reference for COVID-19 control.
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http://dx.doi.org/10.12998/wjcc.v9.i20.5420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281417PMC
July 2021

The efficacy of acoustic therapy versus oral medication for chronic tinnitus: A meta-analysis.

Am J Otolaryngol 2021 Jun 18;42(6):103116. Epub 2021 Jun 18.

Department of Otorhinolaryngology-Head and Neck Surgery, The first hospital of Jilin University, Changchun, Jilin 130021, PR China.

Purpose: To compare the efficacy of acoustic therapy (AT) and drug therapy (DT) for chronic tinnitus.

Methods: We searched Pubmed, ScienceDirect, Chinese Journal Full-text Database (CNKI), Wanfang Database, Chinese Biomedical Literature Database (CBM), Embase, and Cochrane Library from the establishment of the database to December 2019. Meta-analysis was performed on the Tinnitus Handicap Inventory (THI) score and Visual Analogue Scale (VAS) with included literature using Revman 5.3 software.

Results: A total of 18 documents were included, including 16 Chinese documents and 2 English documents, with 1774 patients (including 962 patients treated with AT and 812 patients treated with DT). The effect of AT (by the number of cases or ears) is better than that of DT (P < 0.05). After treatment, the THI value of AT was more evident than that of DT (WMD = -4.25, (-13.24, -5.29)). And the VAS value of AT was significantly lower than that of DT (WMD = -0.73, (-1.31, -0.15)).

Conclusion: Compared with DT, AT can significantly improve the efficacy of tinnitus and reduce the symptoms of tinnitus patients. Clinically, it can vigorously promote the application value of treating tinnitus by sound.
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http://dx.doi.org/10.1016/j.amjoto.2021.103116DOI Listing
June 2021

Fabrication of Two-Dimensional Functional Covalent Organic Frameworks the Thiol-Ene "Click" Reaction as Lubricant Additives for Antiwear and Friction Reduction.

ACS Appl Mater Interfaces 2021 Jul 22. Epub 2021 Jul 22.

State Key Laboratory of Solidification Processing, Center of Advanced Lubrication and Seal Materials, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an 710072, P. R. China.

To address the energy wastage problem caused by friction, novel lubricant additives other than the traditional and basic used additives with outstanding performance are urgently needed. A facile and efficient postsynthetic strategy for modification of two-dimensional (2D) covalent organic frameworks (COFs) was proposed to obtain dialkyl dithiophosphate (DDP)-functionalized COFs ([email protected]) as lubricant additives. The [email protected] was prepared by amine-aldehyde condensation reaction of the triazine compound and vinyl-functionalized monomers through a solvothermal process to form a vinyl-functionalized 2D COF (TD-COF), followed by covalent bonding of commercial lubricating molecules (DDP) the UV-induced thiol-ene "click" reaction. The as-obtained [email protected] with homogeneous distribution of N, P, and S elements exhibits exceptional dispersion stability in the 500SN base oil, which remains stable for over 6 days. With a trace amount addition of 0.05 wt %, superior friction and wear reduction of [email protected] are observed with the friction coefficient lessened to 0.096 from 0.19, wear volume loss declined by 94.9%, and load carrying ability increased from 150 to 650 N simultaneously. The mechanism studies show that the shear force can induce interlayer slipping during the friction process, and the stripped [email protected] can get involved in the contacting interface inducing tribo-chemical reactions N, P, and S elements forming a protective layer on the surfaces. Consequently, the [email protected] demonstrated remarkable friction diminution and abrasion resistance abilities even with a trace amount addition, and this work provides a dependable and valid route for the design and preparation of functional COF-based nanoadditives.
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http://dx.doi.org/10.1021/acsami.1c10459DOI Listing
July 2021

Radical 1,4-Aryl Migration Enabled Remote Cross-Electrophile Coupling of a-Amino-β-Bromo Acid Esters with Aryl Bromides.

Angew Chem Int Ed Engl 2021 Jul 21. Epub 2021 Jul 21.

Hunan University, College of Chemistry and Chemical Engineering, Lushan, 410082, Changsha, CHINA.

Herein, we report an unprecedented, efficient nickel-catalysed radical relay for the remote cross-electrophile coupling of β -bromo- α -benzylamino acid esters with aryl bromides via 1,4-aryl migration/arylation cascades. β -Bromo- α -benzylamino acid esters are considered as unique molecular scaffolds allowing for the aryl migration reactions, which are conceptually novel variants for the radical Truce-Smiles rearrangement. This reaction enables the formation of two new C(sp 3 )-C(sp 2 ) bonds using a bench-stable Ni/bipyridine/Zn system featuring a broad substrate scope and excellent diastereoselectivity, which provides an effective platform for the remote aryl group migration and arylation of amino acid esters via redox-neutral C(sp 3 )-C(sp 2 ) bond cleavage. Mechanistically, this cascade reaction is accomplished by combining two powerful catalytic cycles consisting of a cross-electrophile coupling and radical 1,4-aryl migration through the generation of C(sp 3 )-centred radical intermediates from the homolysis of C(sp 3 )-Br bonds and the switching of the transient alkyl radical into a robust α-aminoalkyl radical.
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http://dx.doi.org/10.1002/anie.202106273DOI Listing
July 2021

Auricular acupressure for insomnia associated with attention deficit hyperactivity disorder: a case report.

Acupunct Med 2021 Jul 20:9645284211026314. Epub 2021 Jul 20.

Fujian Provincial Maternity and Child Health Hospital, Fuzhou, China.

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http://dx.doi.org/10.1177/09645284211026314DOI Listing
July 2021

The probiotic L. casei Zhang slows the progression of acute and chronic kidney disease.

Cell Metab 2021 Jul 8. Epub 2021 Jul 8.

Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, China. Electronic address:

The relationship between gut microbial dysbiosis and acute or chronic kidney disease (CKD) is still unclear. Here, we show that oral administration of the probiotic Lactobacillus casei Zhang (L. casei Zhang) corrected bilateral renal ischemia-reperfusion (I/R)-induced gut microbial dysbiosis, alleviated kidney injury, and delayed its progression to CKD in mice. L. casei Zhang elevated the levels of short-chain fatty acids (SCFAs) and nicotinamide in the serum and kidney, resulting in reduced renal inflammation and damage to renal tubular epithelial cells. We also performed a 1-year phase 1 placebo-controlled study of oral L. casei Zhang use (Chinese clinical trial registry, ChiCTR-INR-17013952), which was well tolerated and slowed the decline of kidney function in individuals with stage 3-5 CKD. These results show that oral administration of L. casei Zhang, by altering SCFAs and nicotinamide metabolism, is a potential therapy to mitigate kidney injury and slow the progression of renal decline.
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http://dx.doi.org/10.1016/j.cmet.2021.06.014DOI Listing
July 2021

The prevalence and genotype distribution of human papilloma virus in cervical squamous intraepithelial lesion and squamous cell carcinoma in Taizhou, China.

Medicine (Baltimore) 2021 Jul;100(28):e26593

Department of Pathology, Taizhou People's Hospital Affiliated to Dalian Medical University, Taizhou, Jiangsu, China.

Abstract: Human papillomavirus (HPV) infection is a common sexually transmitted disease worldwide and the leading cause of cervical cancer. Current vaccines do not cover all HPV genotypes whereas the distribution of HPV genotypes varies in different geographic regions. The study aimed to investigate the distribution of HPV genotypes in patients with cervical squamous intraepithelial lesion (SIL) and cervical squamous cell carcinoma (SCC) in Taizhou City of Jiangsu Province, China. A total of 940 patients including 489 cases with cervical low-grade squamous intraepithelial lesions (LSIL), 356 cases with cervical high-grade squamous intraepithelial lesions (HSIL), and 95 cases with cervical SCC, underwent a biopsy or surgery in Taizhou People's Hospital between January 2019 and December 2019. The HPV testing results were retrospectively analyzed. The overall prevalence of any, high-risk, and low-risk HPV was 83.83%, 81.91%, and 12.13%, respectively. The 5 most common HPV genotypes were HPV16 (35.64%), HPV52 (16.91%), HPV58 (13.94%), HPV33 (8.94%), and HPV18 (7.98%). The prevalence of any and HR-HPV in SCC was significantly higher than those in LSIL and HSIL, while the prevalence of LR-HPV in SCC was significantly lower than those in LSIL and HSIL (P < .01). Single and dual HPV infections were prevalent in SCC, LSIL, and HSIL. Furthermore, the prevalence of dual HPV infection in SCC was significantly higher than those in LSIL and HSIL (P = .002). The HPV prevalence varied by age, being highest among women with SCC, LSIL, and HSIL aged 40 to 49 years, 40 to 49 years, and 50 to 59 years, respectively. In conclusion, the findings revealed a very high prevalence of HPV in women with cervical lesions in Taizhou. Routine HPV tests must cover all common HPV genotypes in clinical practice.
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http://dx.doi.org/10.1097/MD.0000000000026593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284733PMC
July 2021

Association between Genetic Variants in , , and Genes and Risk of Childhood Autism Spectrum Disorder.

Behav Neurol 2021 28;2021:4150926. Epub 2021 Jun 28.

Department of Clinical Laboratory, The Third People's Hospital of Xiaoshan District, Hangzhou, China.

Objective: Genetic factors play an important role in the development of autism spectrum disorder (ASD). This case-control study was to determine the association between childhood ASD and single nucleotide polymorphisms (SNPs) rs3746599 in the gene, rs7794745 in the gene, and rs251379 in the gene in a Chinese Han population.

Methods: Genotypes of SNPs were examined in DNA extracted from blood cells from 201 children with ASD and 200 healthy controls. The Children Autism Rating Scale (CARS) was applied to evaluate the severity of the disease and language impairment. The relationship between SNPs and the risk of ASD or the severity of the disease was determined by logistic regression and one-way ANOVA.

Results: The genotype G/G of rs3746599 in the gene was significantly associated with a decreased risk of ASD (odds ratio (OR) = 0.65, 95% confidence interval (CI): 0.42-0.99, = 0.0449). The T allele of rs7794745 in the gene was associated with an increased risk of ASD (OR = 1.34, 95% CI: 1.01-1.77, = 0.0435). The SNP rs251379 was not associated with ASD. Though none of the SNPs examined were associated with ASD severity, rs7794745 was associated with severity of language impairment.

Conclusions: Our findings suggest that both rs3746599 in the gene and rs7794745 in the gene are associated with risk of childhood ASD, and rs7794745 is also related to the severity of language impairment in autistic children from a Chinese Han population.
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http://dx.doi.org/10.1155/2021/4150926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261179PMC
June 2021

Effects of microplastics on soil organic carbon and greenhouse gas emissions in the context of straw incorporation: A comparison with different types of soil.

Environ Pollut 2021 Jul 8;288:117733. Epub 2021 Jul 8.

State Key Laboratory of Environmental Criteria and Risk Assessment, And State Environmental Protection Key Laboratory of Simulation and Control of Groundwater Pollution, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.

Plastic mulching and straw incorporation are common agricultural practices in China. Plastic mulching is suspected to be a significant source of microplastics in terrestrial environments. Straw incorporation has many effects on the storage of soil organic carbon (SOC) and greenhouse gas emissions, but these effects have not been studied in the presence of microplastic pollution. In this study, 365-day soil incubation experiments were conducted to assess the effects of maize straw and polyethylene microplastics on SOC fractions and carbon dioxide (CO) and nitrous oxide (NO) emissions in two different soils (fluvo-aquic and latosol). Against the background of straw incorporation, microplastics reduced the mineralization and decomposition of SOC, resulting in a microbially available SOC content decrease by 18.9%. In addition, microplastics were carbon-rich, but relatively stable and difficult to be used by microorganisms, thus increasing the mineral-associated SOC content by 52.5%. This indicated that microplastics had adverse effects on microbially available SOC and positive effects on mineral-associated SOC. Microplastics also decreased coarse particulate SOC (>250 μm), and increased non-aggregated silt and clay aggregated SOC (<53 μm). Furthermore, microplastics changed microbial community compositions, thereby reducing the CO and NO emissions of straw incorporation by 26.5%-33.9% and 35.4%-39.7%, respectively. These results showed that microplastics partially offset the increase of CO and NO emissions induced by straw incorporation. Additionally, the inhibitory effect of microplastics on CO emissions in fluvo-aquic soil was lower than that in latosol soil, whereas the inhibitory effect on NO emissions had the opposite trend.
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http://dx.doi.org/10.1016/j.envpol.2021.117733DOI Listing
July 2021

Relation Classification for Bleeding Events From Electronic Health Records Using Deep Learning Systems: An Empirical Study.

JMIR Med Inform 2021 Jul 2;9(7):e27527. Epub 2021 Jul 2.

College of Information and Computer Sciences, University of Massachusetts Amherst, Amherst, MA, United States.

Background: Accurate detection of bleeding events from electronic health records (EHRs) is crucial for identifying and characterizing different common and serious medical problems. To extract such information from EHRs, it is essential to identify the relations between bleeding events and related clinical entities (eg, bleeding anatomic sites and lab tests). With the advent of natural language processing (NLP) and deep learning (DL)-based techniques, many studies have focused on their applicability for various clinical applications. However, no prior work has utilized DL to extract relations between bleeding events and relevant entities.

Objective: In this study, we aimed to evaluate multiple DL systems on a novel EHR data set for bleeding event-related relation classification.

Methods: We first expert annotated a new data set of 1046 deidentified EHR notes for bleeding events and their attributes. On this data set, we evaluated three state-of-the-art DL architectures for the bleeding event relation classification task, namely, convolutional neural network (CNN), attention-guided graph convolutional network (AGGCN), and Bidirectional Encoder Representations from Transformers (BERT). We used three BERT-based models, namely, BERT pretrained on biomedical data (BioBERT), BioBERT pretrained on clinical text (Bio+Clinical BERT), and BioBERT pretrained on EHR notes (EhrBERT).

Results: Our experiments showed that the BERT-based models significantly outperformed the CNN and AGGCN models. Specifically, BioBERT achieved a macro F1 score of 0.842, outperforming both the AGGCN (macro F1 score, 0.828) and CNN models (macro F1 score, 0.763) by 1.4% (P<.001) and 7.9% (P<.001), respectively.

Conclusions: In this comprehensive study, we explored and compared different DL systems to classify relations between bleeding events and other medical concepts. On our corpus, BERT-based models outperformed other DL models for identifying the relations of bleeding-related entities. In addition to pretrained contextualized word representation, BERT-based models benefited from the use of target entity representation over traditional sequence representation.
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http://dx.doi.org/10.2196/27527DOI Listing
July 2021

The leaching behavior of copper and iron recovery from reduction roasting pyrite cinder.

J Hazard Mater 2021 Jul 3;420:126561. Epub 2021 Jul 3.

School of Resources & Safety Engineering, Wuhan Institute of Technology, Wuhan 430073, China. Electronic address:

Pyrite cinder (PyC) is an iron-enriched solid waste material, which is an important iron resource for steel industry. However, the separation or extraction of iron minerals and heavy metals from PyC was ineffective, because of the fine disseminated granularity and the intergrowth between iron minerals and toxic heavy metals during high temperature roasting. In this paper, a novel method to extract copper and iron from the PyC by reduction roasting-leaching-magnetic separation was proposed. The effect of various parameters on the copper leaching behavior were studied, and the corresponding kinetics model was established. Under the optimized leaching conditions, the maximum copper leaching recovery of 82.18% was reached. A high-quality iron concentrate with Fe content of 65.58% and copper content of 0.17% was obtained subsequently from the leaching residuals through magnetic separation. It showed that the leaching process was controlled by mixed diffusion and chemical reaction, with a corresponding activation energy of 27.97 kJ/mol. The free copper oxide, combined copper oxide and secondary copper sulfide were extracted completely in HSO solution. However, chalcopyrite as a form of primary copper sulfide dissolved partly. The leaching mechanism was confirmed by chemical phase and XPS analysis.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126561DOI Listing
July 2021

Aggregation-and-Attention Network for brain tumor segmentation.

BMC Med Imaging 2021 Jul 9;21(1):109. Epub 2021 Jul 9.

College of Computer and Information Science, Fujian Agriculture and Forestry University, Fuzhou, China.

Background: Glioma is a malignant brain tumor; its location is complex and is difficult to remove surgically. To diagnosis the brain tumor, doctors can precisely diagnose and localize the disease using medical images. However, the computer-assisted diagnosis for the brain tumor diagnosis is still the problem because the rough segmentation of the brain tumor makes the internal grade of the tumor incorrect.

Methods: In this paper, we proposed an Aggregation-and-Attention Network for brain tumor segmentation. The proposed network takes the U-Net as the backbone, aggregates multi-scale semantic information, and focuses on crucial information to perform brain tumor segmentation. To this end, we proposed an enhanced down-sampling module and Up-Sampling Layer to compensate for the information loss. The multi-scale connection module is to construct the multi-receptive semantic fusion between encoder and decoder. Furthermore, we designed a dual-attention fusion module that can extract and enhance the spatial relationship of magnetic resonance imaging and applied the strategy of deep supervision in different parts of the proposed network.

Results: Experimental results show that the performance of the proposed framework is the best on the BraTS2020 dataset, compared with the-state-of-art networks. The performance of the proposed framework surpasses all the comparison networks, and its average accuracies of the four indexes are 0.860, 0.885, 0.932, and 1.2325, respectively.

Conclusions: The framework and modules of the proposed framework are scientific and practical, which can extract and aggregate useful semantic information and enhance the ability of glioma segmentation.
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http://dx.doi.org/10.1186/s12880-021-00639-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267236PMC
July 2021

CircUBAP2 Promotes MMP9-Mediated Oncogenic Effect via Sponging miR-194-3p in Hepatocellular Carcinoma.

Front Cell Dev Biol 2021 22;9:675043. Epub 2021 Jun 22.

Zhejiang Provincial Key Laboratory of Laparoscopic Technology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: The physiological regulatory functions of circRNAs have become a topic of intensive research in recent years. Increasing evidence supports a significant role of circRNAs during cancer initiation and progression, including hepatocellular carcinoma (HCC).

Materials And Methods: A bioinformatics analysis from three independent Gene Expression Omnibus (GEO) databases was performed to profile and screen the dysregulated circRNAs in HCC. RT-qPCR was used to examine the expression level of circUBAP2 in HCC and adjacent non-tumor tissues. Then, proliferation assays (CCK8 and colony formation) and migration assays (transwell and wound healing) were performed to examine effect of circUBAP2 . Immunoprecipitation, RNA pulldown, FISH, and dual-luciferase reporter assay was conducted to explore the circUBAP2-related mechanism for regulating HCC progression. Moreover, a mouse xenograft model and a mouse lung metastasis model confirmed the effect of circUBAP2 .

Results: In this study, we found a novel circRNA: circUBAP2, which was identified by bioinformatics analysis. Among 91 HCC patients, circUBAP2 was significantly upregulated in HCC tissues, and negatively correlated with aggressive clinical characteristics and prognosis. Functional assays demonstrated that circUBAP2 promoted cell proliferation, colony formation, migration, and invasion . Moreover, circUBAP2 enhanced tumor growth and pulmonary metastasis . Mechanistically, circUBAP2 acts as a competing endogenous RNA (ceRNA) for miR-194-3p, a tumor suppressor in HCC. We confirmed that MMP9 was direct target for miR-194-3p, which was regulated by circUBAP2.

Conclusion: CircUBAP2 plays a significant role in promoting HCC via the miR-194-3p/MMP9 pathway and could serve as a promising prognostic biomarker and novel therapeutic target for HCC patients.
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http://dx.doi.org/10.3389/fcell.2021.675043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258265PMC
June 2021

Serum pepsinogen II levels are doubled with Helicobacter pylori infection in an asymptomatic population of 40,383 Chinese subjects.

Medicine (Baltimore) 2021 Jul;100(27):e26562

Department of Gastroenterology and Hepatology, Beijing Rehabilitation Hospital, Capital Medical University, Beijing, China.

Abstract: Pepsinogen (PG) I and II are crucial in the gastric digestive processes. This study is to examine the relationship of serum PGI, PGII, and PGI/PGII ratio with Helicobacter pylori (Hp) infection, age, sex, and body mass index (BMI) in subjects in Beijing, China.A total of 40,383 asymptomatic subjects, who underwent medical examination in Beijing Rehabilitation Hospital, were included in this study. Serum PG levels were measured using chemoluminescence techniques. The age, sex, and BMI data were collected, and Hp infection was identified with 13C-urea breath test. Statistical analysis was conducted with Python, Pandas and Seaborn software.Asymptomatic subjects with Hp infection (Hp+) had a significantly higher level of PGI in the serum (111 ng/mL [median]) than those without Hp infection (Hp-) (94 ng/mL, P < .001). The asymptomatic Hp+ subjects had 2-fold higher PGII levels (7.2 ng/mL) than Hp- subjects (3.2 ng/mL, P < .001). These changes produced significantly lower PGI/II ratio in Hp+ patients than in Hp- subjects (16:30, P < .001). The serum PGI and PGII levels were higher in males than in females (PGI: 104 ng/mL vs 95 ng/mL, PGII: 4.3 ng/mL vs 3.7 ng/mL, both P < .001), PGI/II ratio of males is at 95% of that in females (P < .001). PGI and PGII levels gradually increased in older people (P < .001), whereas the PGI/II ratio decreased significantly with age (P < .001). The levels of the two serum PGs were decreased and the ratio increased when BMI were higher than 28 kg/cm2 (P < .05).The levels of serum PGI, especial PGII, were increased by Hp infection, and also influenced by age, sex, and BMI. Therefore, these influencing factors should be considered during clinical practice.
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http://dx.doi.org/10.1097/MD.0000000000026562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270603PMC
July 2021

Experience From 211 Transcortical Selective Amygdalohippocampectomy Procedures: Relevant Surgical Anatomy and Review of the Literature.

Oper Neurosurg (Hagerstown) 2021 Jul 6. Epub 2021 Jul 6.

Department of Neurosurgery, Vanderbilt University Medical Centre, Nashville, Tennessee, USA.

Background: Selective amygdalohippocampectomy (SelAH) is designed to treat medically refractory mesial temporal lobe epilepsy with reduced morbidity compared to standard anterior temporal lobectomy. At our institution, we perform SelAH via a transcortical approach via small corticectomy in the middle temporal gyrus.

Objective: To discuss the surgical anatomy and nuances of SelAH, share our institutional experience, and perform a review of literature.

Methods: Institutional experience was recorded by collecting demographic and outcome data from 1999 to 2017 under an Institutional Review Board protocol in a prospective manner using a REDCap database.

Results: A total of 211 SelAH procedures were performed at our institution between 1999 and 2017. Of these patients, 54% (113/211) were females. The average age at surgery was 39.4 yr. Two-year Engel outcome data were available for 168 patients, of which 73% (123/168) had Engel I outcomes. Engel II outcomes were reported in 16.6% (28/168), III in 4.7% (8/168), and IV in 5.3% (9/168). Our review of literature showed that this is comparable to the seizure freedom rates reported by other groups. We then reviewed our surgical methodology based on operative reports and created illustrations of the surgical anatomy of temporal lobe approach. These illustrations were compared with postoperative magnetic resonance imaging to provide a better 3D understanding of the complex architecture of mesial temporal structures.

Conclusion: SelAH is a minimally invasive, safe, and effective approach for the treatment of medically refractory epilepsy with good surgical outcomes and low morbidity. We feel that mastering the complex anatomy of this approach helps achieve successful outcomes.
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http://dx.doi.org/10.1093/ons/opab206DOI Listing
July 2021

Improvement of cardiac and systemic function in old mice by agonist of growth hormone-releasing hormone.

J Cell Physiol 2021 Jul 5. Epub 2021 Jul 5.

Cardiovascular Key Laboratory of Zhejiang Province, Department of Cardiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Age-related diseases such as cardiovascular diseases portend disability, increase health expenditures, and cause late-life mortality. Synthetic agonists of growth hormone-releasing hormone (GHRH) exhibit several favorable effects on heart function and remodeling. Here we assessed whether GHRH agonist MR409 can modulate heart function and systemic parameters in old mice. Starting at the age of 15 months, mice were injected subcutaneously with MR409 (10 µg/day, n = 8) or vehicle (n = 7) daily for 6 months. Mice treated with MR409 showed improvements in exercise activity, cardiac function, survival rate, immune function, and hair growth in comparison with the controls. More stem cell colonies were grown out of the bone marrow recovered from the MR409-treated mice. Mitochondrial functions of cardiomyocytes (CMs) from the MR409-treated mice were also significantly improved with more mitochondrial fusion. Fewer β-gal positive cells were observed in endothelial cells after 10 passages with MR409. In Doxorubicin-treated H9C2 cardiomyocytes, cell senescence marker p21 and reactive oxygen species were significantly reduced after cultured with MR409. MR409 also improved cellular ATP production and oxygen consumption rate in Doxorubicin-treated H9C2 cells. Mitochondrial protein OPA1 long isoform was significantly increased after treatment with MR409. The effects of MR409 were mediated by GHRH receptor and protein kinase A (PKA). In short, GHRH agonist MR409 reversed the aging-associated changes with respect of heart function, mobility, hair growth, cellular energy production, and senescence biomarkers. The improvement of heart function may be related to a better mitochondrial functions through GHRH receptor/cAMP/PKA/OPA1 signaling pathway and relieved cardiac inflammation.
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http://dx.doi.org/10.1002/jcp.30490DOI Listing
July 2021

Upregulated Expression of Profilin1 on Dendritic Cells in Patients With Severe Aplastic Anemia.

Front Immunol 2021 16;12:631954. Epub 2021 Jun 16.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China.

Severe aplastic anemia (SAA) is a life-threatening form of bone marrow failure that is associated with very high mortality. Dendritic cells (DCs) are antigen presenting cells (APCs) with powerful movement ability, which is an important factor affecting immune function. The expression of profilin1 (Pfn1) plays an important role in the regulation of cell movement ability. We detected the expression of Pfn1 mRNA in the bone marrow (BM) myeloid dendritic cells (mDCs) from patients with SAA using RT-PCR. Next, we examined Pfn1 expression on mDCs using flow cytometry (FCM). We also assessed the relationship between Pfn1 expression and cytokine levels. Our data showed increased Pfn1 mRNA expression in patients with SAA. The expression of Pfn1 in BM mDCs increased in SAA patients. The expression of Pfn1 on mDCs and cytokines (TNF-α and IFN-γ) were positively correlated in the serum of untreated patients with SAA. Taken together, we found that the expression of Pfn1 on mDCs of SAA patients increased, which may affect the function of mDCs. Profilin 1 may be involved in the immunopathogenesis of SAA.
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http://dx.doi.org/10.3389/fimmu.2021.631954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242247PMC
June 2021

Analyzing Genetic Differences Between Sporadic Primary and Secondary/Tertiary Hyperparathyroidism by Targeted Next-Generation Panel Sequencing.

Endocr Pathol 2021 Jul 3. Epub 2021 Jul 3.

Department of Laboratory Medicine, Jeonbuk National University Medical School and Hospital, Jeonju, Republic of Korea.

Secondary hyperparathyroidism (SHPT) is characterized by excessive serum parathyroid hormone levels in response to decreasing kidney function, and tertiary hyperparathyroidism (THPT) is often the result of a long-standing SHPT. To date, several genes have been associated with the pathogenesis of primary hyperparathyroidism (PHPT). However, the molecular genetic mechanisms of uremic hyperparathyroidism (HPT) remain uncharacterized. To elucidate the differences in genetic alterations between PHPT and SHPT/THPT, the targeted next-generation sequencing of genes associated with HPT was performed using DNA extracted from parathyroid tissues. As a result, 26 variants in 19 PHPT or SHPT/THPT appeared as candidate pathogenic mutations, which corresponded to 9 (35%) nonsense, 8 (31%) frameshift, 6 (23%) missense, and 3 (11%) splice site mutations. The MEN1 (23%, 6/26), ASXL3 (15%, 4/26), EZH2 (12%, 3/26), and MTOR (8%, 2/26) genes were frequently mutated. Sixteen of 25 patients with PHPT (64%) had one or more mutations, whereas 3 (21%) of 21 patients with SHPT/THPT had only 1 mutation (p = 0.001). Sixteen of 28 patients (57%) with parathyroid adenoma (PA) had one or more mutations, whereas 3 of 18 patients (17%) with parathyroid hyperplasia (PH) had just one mutation (p = 0.003). Known driver mutations associated with parathyroid tumorigenesis such as CCND1/PRAD1, CDC73/HRPT2, and MEN1 were identified only in PA (44%, 7/16 with mutations). Our results suggest that molecular genetic abnormalities in SHPT/THPT are distinct from those in PHPT. These findings may help in analyzing the molecular pathogenesis underlying uremic HPT development.
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http://dx.doi.org/10.1007/s12022-021-09686-xDOI Listing
July 2021

[Different Responses of Soil Dissolved Organic Matter to Different Types of Compost].

Huan Jing Ke Xue 2021 Jul;42(7):3565-3576

State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

This study explored the responses of soil dissolved organic matter (DOM) to the application of different types of compost using a soil sample without compost as a control. Ultraviolet and fluorescence spectrum technology and EEM-PARAFAC was used to analyze DOM structure and driving factors in soil added with different proportion of cow dung compost (SCC), food and kitchen waste compost (SFC), and sludge compost (SCC). Compared with the control group, contents of AN, NH-N, DOC, and SOM in soil added with compost were significantly increased, and contents of SOM and DOC increased with the increasing of compost amount. When added compost in the same proportion, contents of AN, NO-N, and DOC in SCC and SFC were significantly higher than those in SSC, while contents of NH-N and SOM were higher in SSC. The results of spectral analysis showed that the structure of conjugated benzene ring, hydrophobic component, quinone group, and chromogenic component in DOM of soil added with compost were significantly increased, the transition of unsaturated organic molecule (π→π) was more active, the molecular weight of DOM increased, and the degree of humification was enhanced. When the amount of compost added is 5%, the influence of food and kitchen waste compost on DOM structure was greatest among three types of compost. At 10% and 20%, sludge compost had the greatest impact on DOM structure. The results of EEM-PARAFAC analysis showed that the relative content of fulvic acid-like substances with low molecular in DOM of soil added with compost was increased, while the relative content of proteoid-like substances decreased. 2D-COS analysis showed that compost affected the change order of fluorescence components in DOM. SCC and SFC were as follows:proteoid-like > fulvic acid-like > humus-like; in SSC, it was fulvic acid-like > proteoid-like > humus-like. The enhance of humification and the decrease of relative content of protein-like substances in DOM were related to increased DOC and AN, the relative content of humus-like in low molecular weight was positively correlated with the content of NO-N, and the relative content of macromolecule fulvic acid-like was increased due to the input of SOM from compost.
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http://dx.doi.org/10.13227/j.hjkx.202010037DOI Listing
July 2021

RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in .

Int J Mol Sci 2021 Jun 6;22(11). Epub 2021 Jun 6.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.

Porphyrins are a widespread group of pigments in nature which are believed to contribute to shell colors in mollusks. Previous studies have provided candidate genes for porphyrin shell coloration, however, the linkage analysis between functional genes and porphyrin pigmentation remains unclear in mollusks. RNA interference is a powerful molecular tool for analyzing the loss of functions of genes in vivo and alter gene expression. In this study, we used unicellular alga and as vectors to feed oysters with strain HT115 engineered to express double-stranded RNAs targeting specific genes involved in porphyrin synthesis. A strain of with orange shell was used to target key haem pathway genes expression using the aforementioned approach. We show here that feeding the oysters with , containing dsRNA targeting pigmentation genes, can cause changes in the color of the newly deposited shell. For example, the RNAi knockdown of and resulted in the loss of uroporphyrin pigmentation from the shell due to the accumulation of the pigment in the oyster's mantle. The study probed the crucial role of ALAS and PBGD genes potential functions of uroporphyrin production and shell color pigmentation in .
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http://dx.doi.org/10.3390/ijms22116120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201132PMC
June 2021

Design and analysis of a robust breast cancer diagnostic system based on multimode MR images.

Math Biosci Eng 2021 Apr;18(4):3578-3597

College of Intelligence and Computing, Tianjin University, Tianjin 300350, China.

In this paper, we propose a Robust Breast Cancer Diagnostic System (RBCDS) based on multimode Magnetic Resonance (MR) images. Firstly, we design a four-mode convolutional neural network (FMS-PCNN) model to detect whether an image contains a tumor. The features of the images generated by different imaging modes are extracted and fused to form the basis of classification. This classification model utilizes both spatial pyramid pooling (SPP) and principal components analysis (PCA). SPP enables the network to process images of different sizes and avoids the loss due to image resizing. PCA can remove redundant information in the fused features of multi-sequence images. The best accuracy of this model achieves 94.6%. After that, we use our optimized U-Net (SU-Net) to segment the tumor from the entire image. The SU-Net achieves a mean dice coefficient (DC) value of 0.867. Finally, the performance of the system is analyzed to prove that this system is superior to the existing schemes.
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http://dx.doi.org/10.3934/mbe.2021180DOI Listing
April 2021

Contrast-enhanced CT longitudinal tail sign as a marker of positive resection margins in adenoid cystic carcinoma of the central airway.

J Thorac Dis 2021 May;13(5):2803-2811

Department of Radiology, Jinshan Hospital, Fudan University, Shanghai, China.

Background: Due to submucosal infiltration's biological nature along the airway, adenoid cystic carcinoma (ACC) frequently leaves positive surgical margins. This study evaluated the clinicopathologic, and computed tomography (CT) features for predicting surgical margin status in central airway ACC.

Methods: We retrospectively analyzed the files of 71 patients with ACC of the central airway proven by histopathology and surgery who had presented between January 2010 and December 2018. All patients were classified into positive and negative surgical margin groups according to margin status. Univariate analysis and multivariable logistic regression models were then performed to compare demography, histopathology, and CT characteristics between ACC patients with positive and negative margins.

Results: After surgical resection, 59 (83.1%) patients had positive margins, and 12 (16.9%) had negative margins. The contrast-enhanced CT (CECT) longitudinal tail sign (LTS) was identified in 55 of 59 (93.2%) patients with positive margins and was the only feature that had a significant association with positive margins (odds ratio 41.250, 95% CI: 7.886-215.767; P<0.001). Moreover, positive margins in upper or/and lower directions were associated with the LTS in corresponding directions (P<0.001).

Conclusions: Most central airway ACC patients exhibited positive margins following surgery. The appearance of the LTS on CECT was significantly associated with positive margins and could help preoperatively predict the submucosal invasion of ACC.
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http://dx.doi.org/10.21037/jtd-20-2929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182504PMC
May 2021

Suppressive role of microRNA-130b-3p in ferroptosis in melanoma cells correlates with DKK1 inhibition and Nrf2-HO-1 pathway activation.

Hum Cell 2021 Jun 11. Epub 2021 Jun 11.

Department of Ultrasound, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), No. 61, Jiefang West Road, Changsha, 410005, Hunan Province, People's Republic of China.

Cell death pathways related to ferroptosis are implicated in the progression of melanoma. Emerging data reporting the upregulation of microRNA (miR)-130b-3p in melanoma indicate the potential implication of miR-130b-3p in this malignancy. Herein, we aimed to identify whether and how miR-130b-3p regulated ferroptosis in melanoma cells. Melanoma cells (A375, G-361) were treated with erastin or RSL3 to mimic ferroptosis in vitro. Viability, lipid peroxidation level and ferrous ion content in melanoma cells were then assessed in response to manipulation of miR-130b-3p expression. Luciferase assay was conducted to determine the binding of miR-130b-3p to Dickkopf1 (DKK1). Western blot assay was conducted to determine the expression of molecules related to nuclear factor-erythroid 2 p45-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway. The results indicated that miR-130b-3p exerted an inhibitory role in erastin or RSL3-induced ferroptosis, evidenced by reductions in lipid peroxidation and ferrous ion content. By suppressing the expression of target gene DKK1, miR-130b-3p activated the Nrf2/HO-1 pathway, whereby repressing ferroptosis. miR-130b-3p blocked the antitumor activity of erastin. Further, in vitro findings were reproduced in an in vivo murine model. Together, these data suggest the potential of miR-130b-3p to inhibit ferroptosis in melanoma cells and the mechanism was related to DKK1-mediated Nrf2/HO-1 pathway.
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http://dx.doi.org/10.1007/s13577-021-00557-5DOI Listing
June 2021

Endogenous bacteria inhabiting the Ophiocordyceps highlandensis during fruiting body development.

BMC Microbiol 2021 Jun 11;21(1):178. Epub 2021 Jun 11.

Yunnan Herbal Laboratory, School of Ecology and Environmental Science, Yunnan University, Kunming, 650091, China.

Background: The genus Ophiocordyceps, which includes Ophiocordyceps sinensis, has been demonstrated to be one of the most valuable medicinal taxa. The low rate of larval infection and slow development that characterize the cultivation of this genus should be urgently addressed. To identify potential bioinoculants that stimulate the growth of Ophiocordyceps, O. highlandensis was selected as a model system, and a total of 72 samples were collected to systematically compare the microbial communities present during fruiting body development. By applying high-throughput 16S and ITS2 amplicon sequencing technology, the bacterial and fungal communities were identified in O. highlandensis and its surrounding soil, and the functional dynamics of the bacteria were explored.

Results: The results indicate that the most abundant bacteria across all the samples from O. highlandensis were Proteobacteria, Firmicutes and Bacteroidetes, while members of Ascomycota were detected among the fungi. The pathways enriched in the developmental stages were associated with carbohydrate degradation, nucleotides and pyridoxal biosynthesis, and the TCA cycle. Compared with that in the fungal community, an unexpectedly high taxonomic and functional fluctuation was discovered in the bacterial community during the maturation of O. highlandensis. Furthermore, bipartite network analysis identified four potential supercore OTUs associated with O. highlandensis growth.

Conclusions: All the findings of this study suggest unexpectedly high taxonomic and functional fluctuations in the bacterial community of O. highlandensis during its maturation. O. highlandensis may recruit different endogenous bacteria across its life cycle to enhance growth and support rapid infection. These results may facilitate Ophiocordyceps cultivation and improve the development of strategies for the identification of potential bioinoculant resources.
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http://dx.doi.org/10.1186/s12866-021-02227-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196446PMC
June 2021

Interrelationships Between Oxidative Stress, Cytokines, and Psychotic Symptoms and Executive Functions in Patients With Chronic Schizophrenia.

Psychosom Med 2021 Jun;83(5):485-491

From the Department of Psychiatry, Shenzhen Kangning Hospital (Wu); Shenzhen Mental Health Center (Wu). Shenzhen, Guangdong; Yantai Automobile Engineering Professional College (Yu), Yantai; Department of Psychiatry (Wang, Guan), Hebei Province Rong-Jun Hospital, Baoding; Psychiatry Research Center, Beijing HuiLongGuan Hospital, Peking University HuiLongGuan Clinical Medical School (Xiu); and CAS Key Laboratory of Mental Health, Institute of Psychology (Zhang), Chinese Academy of Sciences, Beijing, China.

Objective: Accumulating evidence has demonstrated that the pathophysiology of schizophrenia is involved in various abnormalities in oxidative stress markers and cytokines closely related to synaptic plasticity. However, the interactive effects among key cytokines, oxidative stress, and executive dysfunction and symptoms of schizophrenia have not been investigated yet.

Methods: A total of 189 patients with chronic schizophrenia and 60 controls were recruited in the current study. Tumor necrosis factor α (TNF-α), interleukin (IL)-8, IL-6, and IL-2 levels; catalase, glutathione peroxidase, and superoxide dismutase (SOD) activities; and malondialdehyde (MDA) levels were determined in patients and controls. Executive function was evaluated by the Wisconsin card sorting tests, the verbal fluency tests, and the Stroop word-color test. Clinical symptoms were evaluated by the Positive and Negative Syndrome Scale.

Results: Relative to the controls, the patients had lower activities of SOD and glutathione peroxidase and levels of TNF-α, but higher levels of MDA, IL-8, IL-6, and IL-2 (all p values < .05). A significant negative relationship between SOD activity and IL-8 levels was found only in patients (β = -0.44, p = .008). Furthermore, we found that an interactive effect of low TNF-α level and high MDA level was associated with negative symptoms (β = -0.02, p = .01). Moreover, the interactive effects of IL-8 and MDA or IL-8 and SOD were correlated with executive function only in patients (β = 0.23, p = .02; β = 0.09, p = .03).

Conclusions: Our findings suggest that the interrelationships between oxidative stress markers and cytokines occur in schizophrenia patients, which may be the basis of their pathological mechanisms underlying clinical symptoms and cognitive dysfunction.
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http://dx.doi.org/10.1097/PSY.0000000000000931DOI Listing
June 2021

Nano-Sized Hydroxyapatite Induces Apoptosis and Osteogenic Differentiation of Vascular Smooth Muscle Cells via JNK/c-JUN Pathway.

Int J Nanomedicine 2021 27;16:3633-3648. Epub 2021 May 27.

Department of Cardiology, Cardiovascular Key Laboratory of Zhejiang Province, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, 310009, People's Republic of China.

Purpose: The deposition of hydroxyapatite (HAp) crystals plays an important role in the development of vascular calcification (VC). This study aimed to demonstrate the effects of nanosized HAp (nHAp) on vascular smooth muscle cells (VSMCs) and VC progression.

Methods: Transmission electron microscopy (TEM) was used to examine cellular uptake of nHAp. Cell viability was determined using CCK-8 assay kit. Mitochondrial impairment and reactive oxygen species were detected by TEM and fluorescence dye staining, respectively. Cell apoptosis was detected by Western blot analysis and Annexin V staining. Mouse model of VC was built via applying nHAp on the surface of abdominal aorta. Calcification was visualized by Alizarin red and von Kossa staining.

Results: We found that nHAp could promote osteogenic transformation of VSMCs by elevating expression of runt-related factor 2 (Runx2), osteopontin (OPN) and alkaline phosphatase (ALP), impairing function and morphology of mitochondria and inducing apoptosis of VSMCs. More phosphorylation of c-Jun N-terminal protein kinase/c-JUN (JNK/c-JUN) in VSMCs was detected after mixing nHAp with VSMCs. HAp-induced osteogenic transformation of VSMCs was blocked by JNK inhibitor SP600125, resulted in decreased ALP activity, less Runx2 and OPN expressions. SP600125 also inhibited apoptosis of VSMCs. Application of nHAp to outside of aorta induced osteogenic transformation and apoptosis of VSMCs, and significant deposition of calcium on the vessel walls of mice, which can be effectively attenuated by SP600125.

Conclusion: JNK/c-JUN signaling pathway is critical for nHAp-induced calcification, which could be a potential therapeutic target for controlling the progression of VC.
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http://dx.doi.org/10.2147/IJN.S303714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166281PMC
June 2021

Nonvolatile Optically Reconfigurable Radiative Metasurface with Visible Tunability for Anticounterfeiting.

Nano Lett 2021 Jun 2;21(12):5269-5276. Epub 2021 Jun 2.

State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, China.

Control of thermal emission underpins fundamental science, as it is related to both heat and infrared electromagnetic wave transport. However, realizing nonvolatile reconfigurable thermal emission is challenging due to the inherent complexity or limitation in conventional radiative materials or structures. Here, we experimentally demonstrate a nonvolatile optically reconfigurable mid-infrared coding radiative metasurface. By applying laser pulses, infrared emissive patterns are directly encoded into an ultrathin (∼25 nm) GeSbTe layer integrated into a planar optical cavity with the optically crystallized GeSbTe spots, and the peak spectral emissivity is repeatedly switched between low (∼0.1) and high (∼0.7) values. In addition, the visible scattering patterns are independently modulated with submicron-sized bumps generated by high-power laser pulses. An anticounterfeiting label is demonstrated with spatially different infrared emission and visible light scattering information encoded. This approach constitutes a new route toward thermal emission control and has broad applications in encryption, camouflage, and so on.
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http://dx.doi.org/10.1021/acs.nanolett.1c01396DOI Listing
June 2021

Comparative Analysis of Mitochondrial Genome Features among Four Species and Insight into Their Systematic Positions in the Order Hypocreales.

Int J Mol Sci 2021 May 24;22(11). Epub 2021 May 24.

College of Ecology and Environmental Sciences, Yunnan University, Kunming 650091, China.

The mycoparasite fungi of have contributed to the biological control of plant fungal disease and nematodes. The fungi strains were isolated from , and soil, which identified as , , and sp. To explore the evolutionary relationship between the mentioned species, the mitochondrial genomes of four species were sequenced and assembled. The four mitogenomes consisted of complete circular DNA molecules, with the total sizes ranging from 27,410 bp to 42,075 bp. The GC contents, GC skews and AT skews of the mitogenomes varied considerably. Mitogenomic synteny analysis indicated that these mitogenomes underwent gene rearrangements. Among the 15 protein-coding genes within the mitogenomes, the nad4L gene exhibited the least genetic distance, demonstrating a high degree of conservation. The selection pressure analysis of these 15 PCGs were all below 1, indicating that PCGs were subject to purifying selection. Based on protein-coding gene calculation of the significantly supported topologies, the four species were divided into a group in the phylogenetic tree. The results supplemented the database of mitogenomes in Hypocreales order, which might be a useful research tool to conduct a phylogenetic analysis of . Additionally, the suitable molecular marker was significant to study phylogenetic relationships in the Bionectriaceae family.
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http://dx.doi.org/10.3390/ijms22115530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197242PMC
May 2021
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