Publications by authors named "Hong Yu"

1,992 Publications

  • Page 1 of 1

Repetitive Transcranial Magnetic Stimulation for Neuropathic Pain and Neuropsychiatric Symptoms in Traumatic Brain Injury: A Systematic Review and Meta-Analysis.

Neural Plast 2022 30;2022:2036736. Epub 2022 Jul 30.

Department of Rehabilitation, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.

Neuropathic pain and neuropsychiatric symptoms are common complications reported by the traumatic brain injury (TBI) population. Although a growing body of research has indicated the effectiveness of repetitive transcranial magnetic stimulation (rTMS) for the management of neurological and psychiatric disorders, little evidence has been presented to support the effects of rTMS on neuropathic pain and neuropsychiatric symptoms in patients with TBI in all age groups. In addition, a better understanding of the potential factors that might influence the therapeutic effect of rTMS is necessary. The objective of this preregistered systematic review and meta-analysis was to quantify the effects of rTMS on physical and psychological symptoms in individuals with TBI. We systematically searched six databases for randomized controlled trials (RCTs) of rTMS in TBI patients reporting pain and neuropsychiatric outcomes published until March 20, 2022. The mean difference (MD) with 95% confidence intervals (CIs) was estimated separately for outcomes to understand the mean effect size. Twelve RCTs with 276 TBI patients were ultimately selected from 1605 records for systematic review, and 11 of the studies were included in the meta-analysis. Overall, five of the included studies showed a low risk of bias. The effects of rTMS on neuropathic pain were statistically significant (MD = -1.00, 95% CI -1.76 to -0.25, = 0.009), with high heterogeneity ( = 76%). A significant advantage of 1 Hz rTMS over the right dorsolateral prefrontal cortex (DLPFC) in improving depression (MD = -6.52, 95% CI -11.58 to -1.46, = 0.01) was shown, and a significant improvement was noted in the Rivermead Post-Concussion Symptoms Questionnaire-13 (RPQ-13) scores of mild TBI patients after rTMS (MD = -5.87, 95% CI -10.63 to -1.11, = 0.02). However, no significance was found in cognition measurement. No major adverse events related to rTMS were reported. Moderate evidence suggests that rTMS can effectively and safely improve neuropathic pain, while its effectiveness on depression, postconcussion symptoms, and cognition is limited. More trials with a larger number of participants are needed to draw firm conclusions. This trial is registered with PROSPERO (PROSPERO registration number: CRD42021242364.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/2036736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357260PMC
August 2022

Selection and characterization of plant-derived alkaloids with strong antialgal inhibition: growth inhibition selectivity and inhibitory mechanism.

Harmful Algae 2022 Aug 21;117:102272. Epub 2022 Jun 21.

Beijing Key Laboratory for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China; Engineering Research Center for Water Pollution Source Control & Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China.

In recent years, researches on microalgae inhibition with plant-derived active substances have attracted much attention. In this study, the inhibition of six plant-derived alkaloids (neferine, isoliensinine, linensinine, nuciferine, capsaicin, and hordenine) on bloom-forming cyanobacteria Microcystis aeruginosa were investigated. The results showed that neferine and nuciferine had stronger inhibition on the growth of M. aeruginosa compared with the other four alkaloids, and the relative inhibition rate reached 91.27% and 88.70% at the concentration of 4.5 mg/L after 7 d of exposure, respectively. Different from neferine, nuciferine has no inhibition on Chlorella sp. and Tetradesmus obliquus. It also increased the diversity and species homogeneity of phytoplankton in the environmental water samples. Nuciferine decreased the contents of chlorophyll a and β-carotene in M. aeruginosa with the extension of treatment time, which was 59.40% and 31.90% of the control at the concentration of 1.04 mg/L after 48 h, respectively. After 48 h of nuciferine exposure, the values of fluorescence parameters including maximum quantum yield (Fv/Fm), actual quantum yield of PSII (Yield), non-photochemical quenching (qN and NPQ), and electron transport rates (ETR) of M. aeruginosa cells were significantly decreased and photosynthetic capacity was weakened. The superoxide dismutase (SOD), catalase (CAT), ascorbic acid (ASA), and glutathione (GSH) in the cells were significantly reduced, and the hydrogen peroxide (HO) and malonaldehyde (MDA) contents continued to accumulate, causing severe oxidative damage. Therefore, the good biological safety and strong specific inhibition of nuciferine makes it have great application prospects in the inhibition of cyanobacteria blooms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.hal.2022.102272DOI Listing
August 2022

Patient-controlled intravenous analgesia with opioids after thoracoscopic lung surgery: a randomized clinical trial.

BMC Anesthesiol 2022 Aug 8;22(1):253. Epub 2022 Aug 8.

Department of Anesthesiology, West China Hospital, Sichuan University. No, 37 Guoxue Alley, Chengdu, 610041, China.

Background: Opioids remain the mainstream therapy for post-surgical pain. The choice of opioids administered by patient-controlled intravenous analgesia (PCIA) for thoracoscopic lung surgery is unclear. This study compared 3 opioid analgesics for achieving satisfactory analgesia with minimal emesis (SAME).

Methods: This randomized clinical trial enrolled patients scheduled for thoracoscopic lung surgery randomized to receive 1 of 3 opioids for PCIA: oxycodone (group O), hydromorphone (group H), and sufentanil (group S). The primary outcome was the proportion of subjects achieving SAME, i.e., no-to-mild pain (pain score < 4/10) with minimal nausea/vomiting (PONV score < 2/4) when coughing during the pulmonary rehabilitation exercise in the first 3 postoperative days.

Results: Of 555 enrolled patients, 184 patients in group O, 186 in group H and 184 in group S were included in the final analysis. The primary outcome of SAME was significantly different among group O, H and S (41.3% vs 40.3% vs 29.9%, P = 0.043), but no difference was observed between pairwise group comparisons. Patients in groups O and H had lower pain scores when coughing on the second day after surgery than those in group S, both with mean differences of 1 (3(3,4) and 3(3,4) vs 4(3,4), P = 0.009 and 0.039, respectively). The PONV scores were comparable between three groups (P > 0.05). There were no differences in other opioid-related side effects, patient satisfaction score, and QoR-15 score among three groups.

Conclusions: Given clinically relevant benefits detected, PCIA with oxycodone or hydromorphone is superior to sufentanil for achieving SAME as a supplement to multimodal analgesia in patients undergoing thoracoscopic lung surgery.

Trial Registration: This study was registered at ( ChiCTR2100045614 , 19/04/2021).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12871-022-01785-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358799PMC
August 2022

Characterization of larval shell formation and CgPOU2F1, CgSox5, and CgPax6 gene expression during shell morphogenesis in Crassostrea gigas.

Authors:
Yue Min Qi Li Hong Yu

Comp Biochem Physiol B Biochem Mol Biol 2022 Aug 1;263:110783. Epub 2022 Aug 1.

Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, and College of Fisheries, Ocean University of China, Qingdao 266003, China.

Shell formation is a dynamic process involving organic matrix secretion and calcification. In this study, we characterized shell morphogenesis during larval development in Crassostrea gigas. Using scanning electron microscopy (SEM) and fluorescence staining, we demonstrated that shell field, the first morphologically distinguishable shell-forming tissue, became visible soon after enlargement of the blastopore at the anterior end of the trochophore. Shell organic matrix namely protein polysaccharides and calcified structure appeared as a slit at the dorsal side of the embryo. The early shell field began to extend along the dorsal side of the trochophore larvae, and became a saddle shaped shell field that gave rise to the prodissoconch I embryonic shell in the early D-shaped larvae. Subsequently, prodissoconch II shell was formed in the late D-shaped larvae with a characteristic appearance of growth lines. To identify gene expression markers for studying shell formation, we isolated three potential larval shell formation genes CgPOU2F1, CgSox5, and CgPax6 and analyzed their expression during shell morphogenesis. The three potential shell formation genes possessed a similar pattern of expression. Their expression was detected in the shell gland and shell field regions in early D-shaped larvae, hereafter, their expression was detected at the larval mantle edge in the calcified shell stages. Together, these studies provide knowledge of shell morphogenesis in pacific oyster and molecular markers for studying the molecular regulation of biomineralization and shell formation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpb.2022.110783DOI Listing
August 2022

The TBK1/IKKε inhibitor amlexanox improves dyslipidemia and prevents atherosclerosis.

JCI Insight 2022 Aug 2. Epub 2022 Aug 2.

Department of Medicine, University of California San Diego, La Jolla, United States of America.

Cardiovascular diseases, especially atherosclerosis and its complications, are a leading cause of death. Inhibition of the non-canonical IκB kinases TBK1 and IKKε with amlexanox restores insulin sensitivity and glucose homeostasis in diabetic mice and human subjects. Here we report that amlexanox improves diet-induced hypertriglyceridemia and hypercholesterolemia in Western diet (WD)-fed Ldlr-/- mice, and protects against atherogenesis. Amlexanox ameliorates dyslipidemia, inflammation and vascular dysfunction through synergistic actions that involve upregulation of bile acid synthesis to increase cholesterol excretion. Transcriptomic profiling demonstrates an elevated expression of key bile acid synthesis genes. Furthermore, we found that amlexanox attenuates monocytosis, eosinophilia and vascular dysfunction during WD-induced atherosclerosis. These findings demonstrate the potential of amlexanox as a new therapy for hypercholesterolemia and atherosclerosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1172/jci.insight.155552DOI Listing
August 2022

Selection and colorimetric application of ssDNA aptamers against metamitron based on magnetic bead-SELEX.

Anal Methods 2022 Aug 11;14(31):3021-3032. Epub 2022 Aug 11.

Shanghai Jiao Tong University, YunNan (Dali) Research Institute, Dali, Yunnan 671000, China.

Metamitron (MTM) is a typical and widely used triazine herbicide in agricultural production. Its moderate toxicity and high residue in the environment have deleterious impacts on human health. The establishment of a rapid and efficient MTM detection method is of great significance. In this study, a magnetic-bead SELEX (systematic evolution of ligands by exponential enrichment) system was developed to select the MTM aptamers with high affinity and specificity. Through 10 rounds of screening, six candidate aptamers with the highest abundance were obtained by high-throughput sequencing. The homology, secondary structure, and affinity analyses were performed. The aptamer named MTM-6 was selected as the optimal aptamer with the dissociation constant () value of 16 nM. Then, a colorimetric detection method for MTM based on aptamer MTM-6 and the aggregation of gold nanoparticles (AuNPs) induced by NaCl was established with a linear range from 20 to 1000 nM ( = 0.9966) and a limit of detection (LOD) of 4.58 nM. The average recovery rate of MTM in the application of actual aqueous samples ranged from 95.40 to 107.83% with a relative standard deviation (RSD) from 1.11 to 3.48%. With considerable sensitivity and specificity, this colorimetric aptasensor is convenient and efficient, and shows bright application potential in MTM detection in aqueous samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2ay00566bDOI Listing
August 2022

Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection.

FASEB J 2022 Sep;36(9):e22468

State Key Laboratory of Nature Medicines, Key Laboratory of Drug Metabolism and Pharmacokinetic, China Pharmaceutical University, Nanjing, China.

Cholestasis is a common complication of hepatitis B virus (HBV) infection, characterized by increased intrahepatic and plasma bile acid levels. Cholestasis was found negatively associated with hepatitis outcome, however, the exact mechanism by which cholestasis impacts anti-viral immunity and impedes HBV clearance remains elusive. Here, we found that cholestatic mice are featured with dysfunctional T cells response, as indicated by decreased sub-population of CD25 /CD69 CD4 and CD8 cells, while CTLA-4 CD4 and CD8 subsets were increased. Mechanistically, bile acids disrupt intracellular calcium homeostasis via inhibiting mitochondria calcium uptake and elevating cytoplasmic Ca concentration, leading to STIM1 and ORAI1 decoupling and impaired store-operated Ca entry which is essential for NFAT signaling and T cells activation. Moreover, in a transgenic mouse model of HBV infection, we confirmed that cholestasis compromised both CD4 and CD8 T cells activation resulting in poor viral clearance. Collectively, our results suggest that bile acids play pivotal roles in anti-HBV infection via controlling T cells activation and metabolism and that targeting the regulation of bile acids may be a therapeutic strategy for host-virus defense.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.202200332RDOI Listing
September 2022

The association between the pre-pregnancy vaginal microbiome and time-to-pregnancy: a Chinese pregnancy-planning cohort study.

BMC Med 2022 08 1;20(1):246. Epub 2022 Aug 1.

Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, No. 87 Dingjiaqiao Rd., Gulou District, Nanjing, Jiangsu, China.

Background: Although sexually transmitted infections are regarded as the main cause of tubal infertility, the association between the common vaginal microbiome and female fecundability has yet to be determined. The objective of this study was to find convincing evidence relating to the impact of the vaginal bacterial structure on the fecundability of women planning pregnancy.

Methods: We recruited women who took part in the Free Pre-pregnancy Health Examination Project from 13 June 2018 to 31 October 2018 (n = 89, phase I) and from 1 November 2018 to 30 May 2020 (n = 389, phase II). We collected pre-pregnancy vaginal swabs from each subject; then, we followed up each subject to acquire the pregnancy-planning outcome in 1 year. In phase I, 16S rRNA gene sequencing was performed to investigate the vaginal bacterial content between the pregnancy and non-pregnancy groups. These findings were verified in phase II by applying a quantitative real-time polymerase chain reaction for the measurement of the absolute abundance of specific species. Cox models were used to estimate fecundability ratios (FR) for each vaginal microbiome type.

Results: In phase I, 59.6% (53/89) of women became pregnant within 1 year. The principal coordinate analysis showed that the pre-pregnancy vaginal microbial community structures of the pregnant and non-pregnant groups were significantly different (PERMANOVA test, R = 0.025, P = 0.049). The abundance of the genus Lactobacillus in the pregnancy group was higher than that of the non-pregnant group (linear discriminant analysis effect size (LDA) > 4.0). The abundance of the genus Gardnerella in the non-pregnant group was higher than those in the pregnant group (LDA > 4.0). In phase II, female fecundability increased with higher absolute loads of Lactobacillus gasseri (quartile Q4 vs Q1, FR = 1.71, 95%CI 1.02-2.87) but decreased with higher absolute loads of Fannyhessea vaginae (Q4 vs Q1, FR = 0.62, 95%CI 0.38-1.00). Clustering analysis showed that the vaginal microbiome of type D (characterized by a higher abundance of Lactobacillus iners, a lower abundance of Lactobacillus crispatus and Lactobacillus gassri) was associated with a 55% reduction of fecundability (FR = 0.45, 95%CI 0.26-0.76) compared with type A (featuring three Lactobacillus species, low Gardnerella vaginalis and Fannyhessea vaginae abundance).

Conclusions: This cohort study demonstrated an association between the pre-pregnancy vaginal microbiome and female fecundability. A vaginal microbiome characterized by a higher abundance of L. iners and lower abundances of L. crispatus and L. gasseri appeared to be associated with a lower fecundability. Further research now needs to confirm whether manipulation of the vaginal microenvironment might improve human fecundability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12916-022-02437-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341075PMC
August 2022

Prognostic and predictive value of radiomic signature in stage I lung adenocarcinomas following complete lobectomy.

J Transl Med 2022 07 28;20(1):339. Epub 2022 Jul 28.

Department of Radiology, Shanghai Chest Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: The overall survival (OS) of stage I operable lung cancer is relatively low, and not all patients can benefit from adjuvant chemotherapy. This study aimed to develop and validate a radiomic signature (RS) for prediction of OS and adjuvant chemotherapy candidates in stage I lung adenocarcinoma.

Methods: A total of 474 patients from 2 centers were divided into 1 training (n = 287), 1 internal validation (n = 122), and 1 external validation (n = 65) cohorts. We extracted 1218 radiomic features from preoperative CT images and constructed RS. We further investigated the prognostic value of the RS in survival analysis. Interaction between treatment and RS was assessed to evaluate its predictive value. Propensity score matching (PSM) was conducted.

Results: Overall, 474 eligible patients with stage I lung adenocarcinoma (214 men [45.1%]; median age, 60 years) were identified. The RS was significantly associated with OS in the training and two validation cohorts (hazard ratios [HRs]  >  = 3.22). In multivariable analysis, the RS remained an independent prognostic factor adjusting for clinicopathologic variables (adjusted HRs >  = 2.63). The prognostic value of RS was also confirmed in PSM analysis. In stage I patients, the interaction between RS status and adjuvant chemotherapy was significant (interaction P = 0.020). Within the stratified analysis, good chemotherapy efficacy was only observed for patients with stage IB disease (interaction P < 0.001).

Conclusions: Our results suggested that the radiomic signature was associated with overall survival in patients with stage I lung adenocarcinoma and might predict adjuvant chemotherapy benefit, especially in stage IB patients. The potential of radiomic signature as a noninvasive predictor needed to be confirmed in future studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12967-022-03547-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331779PMC
July 2022

Morphological and Phylogenetic Characterization Reveals Five New Species of (Cordycipitaceae, Hypocreales).

J Fungi (Basel) 2022 Jul 19;8(7). Epub 2022 Jul 19.

Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650091, China.

is a very important fungal resource, with some species in the genus having great medical, economic and ecological value. This study reports five new species of from Yunnan Province and Guizhou Province in Southwestern China and Dole Province in Vietnam, providing morphological descriptions, illustrations, phylogenetic placements, associated hosts and comparisons with allied taxa. Based on morphological observations and phylogenetic analyses of combined nr, nr, , and sequence data, it was determined that these five new species were located in the clade of and different from other species of . The five novel species had morphologies similar to those of other species in the genus, with bright orange cylindrical to clavate stromata (gregarious). The fertile part lateral sides usually had a longitudinal ditch without producing perithecia, and superficial perithecia. The phialides had a swollen basal portion, tapering abruptly into a narrow neck and oval or fusiform one-celled conidia, often in chains. The morphological characteristics of 23 species in , including five novel species and 18 known taxa, were also compared in the present study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/jof8070747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321185PMC
July 2022

Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice.

Cell Discov 2022 Jul 26;8(1):71. Epub 2022 Jul 26.

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.

Chilling is a major abiotic stress harming rice development and productivity. The C-REPEAT BINDING FACTOR (CBF)-dependent transcriptional regulatory pathway plays a central role in cold stress and acclimation in Arabidopsis. In rice, several genes have been reported in conferring chilling tolerance, however, the chilling signaling in rice remains largely unknown. Here, we report the chilling-induced OSMOTIC STRESS/ABA-ACTIVATED PROTEIN KINASE 6 (OsSAPK6)-IDEAL PLANT ARCHITECTURE 1 (IPA1)-OsCBF3 signal pathway in rice. Under chilling stress, OsSAPK6 could phosphorylate IPA1 and increase its stability. In turn, IPA1 could directly bind to the GTAC motif on the OsCBF3 promoter to elevate its expression. Genetic evidence showed that OsSAPK6, IPA1 and OsCBF3 were all positive regulators of rice chilling tolerance. The function of OsSAPK6 in chilling tolerance depended on IPA1, and overexpression of OsCBF3 could rescue the chilling-sensitive phenotype of ipa1 loss-of-function mutant. Moreover, the natural gain-of-function allele ipa1-2D could simultaneously enhance seedling chilling tolerance and increase grain yield. Taken together, our results revealed a chilling-induced OsSAPK6-IPA1-OsCBF signal cascade in rice, which shed new lights on chilling stress-tolerant rice breeding.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41421-022-00413-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325753PMC
July 2022

Rapid and sensitive detection of adulteration in by recombinase polymerase amplification combined with lateral flow strip.

Acta Biochim Biophys Sin (Shanghai) 2022 Jul;54(7):1-4

Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Jiangsu Ocean University, Lianyungang, 222005, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3724/abbs.2022079DOI Listing
July 2022

Expression of tyrosinase-like protein genes and their functional analysis in melanin synthesis of Pacific oyster (Crassostrea gigas).

Gene 2022 Oct 19;840:146742. Epub 2022 Jul 19.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.

Color polymorphism in Mollusca is of great interest for consumer preference. Although the heritability of shell color variation has been conducted by experimental crossing, little is known about molecular basis involved in these patterns. Tyrosinase-like proteins are important enzymes which are members of the type-3 copper protein superfamily. In this research, two tyrosinase-like protein genes including CgTyp-1 and CgTyp-3 were identified in the Pacific oyster Crassostrea gigas. Tissue expression analysis showed that CgTyp-1 and CgTyp-3 were dominantly expressed in the mantle. Particularly, they were expressed significantly higher in the edge mantle than that in the central mantle whether on the left or right mantles. Additionally, expressions of CgTyp-1 and CgTyp-3 were mainly found in the black shell color oysters, with relative lower levels in the white shell color oysters. In situ hybridization showed that positive signals for CgTyp-1 and CgTyp-3 were both detected within the outer epithelium of the outer fold either in the black or white shell color oysters. After interference, the expression levels of CgTyp-1 and CgTyp-3 mRNA were significantly attenuated, and the efficiency of RNAi reached 84.72% and 71.58%, respectively. Besides, knockdown CgTyp-1 or CgTyp-3, obviously decreased the tyrosinase activity of mantles. Furthermore, the number of the melanosomes within epithelium of the outer fold was sharply reduced by silencing of each Typ. These findings argue that CgTyp-1 and CgTyp-3 may be involved in the melanin synthesis, which lends insight into regulation mechanism of shell pigmentation in C. gigas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2022.146742DOI Listing
October 2022

Effect of Volatile Anesthesia Versus Total Intravenous Anesthesia on Postoperative Pulmonary Complications in Patients Undergoing Cardiac Surgery: A Randomized Clinical Trial.

J Cardiothorac Vasc Anesth 2022 Jun 19. Epub 2022 Jun 19.

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China. Electronic address:

Objectives: The purpose of this study was to evaluate the effect of volatile anesthesia and propofol-based total intravenous anesthesia (TIVA) on postoperative pulmonary complications (PPCs) among patients undergoing cardiac surgery.

Design: Parallel-group, randomized controlled trial.

Setting: Single-center tertiary care hospital.

Participants: Five hundred twenty-four patients undergoing cardiac surgery with cardiopulmonary bypass.

Interventions: The patients were assigned randomly (1:1) to receive anesthesia maintenance with a volatile anesthetic (sevoflurane or desflurane) or propofol-based TIVA.

Measurements And Main Results: The primary outcome was a composite of postoperative pulmonary complications within the first 7 postoperative days. The PPCs occurred in 118 of 262 patients (45.0%) in the volatile anesthesia group compared with 105 of 262 patients (40.1%) in the propofol-based intravenous anesthesia group (relative risk: 1.17 [95% CI 0.96-1.42], p = 0.123). There were no significant differences in the severity of PPCs within 7 days postoperatively, the occurrence and severity grade of PPCs within 30 days, the incidence of hypoxia, and 30-day mortality.

Conclusions: In adult patients undergoing cardiac surgery with cardiopulmonary bypass, general anesthesia with a volatile anesthetic compared with propofol-based TIVA had not reduced pulmonary complications within the first 7 days after surgery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1053/j.jvca.2022.06.014DOI Listing
June 2022

Dietary iron modulates hepatic glucose homeostasis via regulating gluconeogenesis.

J Nutr Biochem 2022 Jul 19;109:109104. Epub 2022 Jul 19.

MoE Key Laboratory of Molecular Animal Nutrition, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China; Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address:

Iron exerts significant influences on glucose metabolism. However, the regulatory mechanisms underlying disordered glucose response remains largely unclear. The aim of this study was to examine the impact of dietary iron on hepatic gluconeogenesis in mice and in rat liver-derived cells. High iron models of C57BL/6J mice were fed with 1.25 g Fe/kg diets for 9 weeks, and high-iron BRL-3A cell models were treated with 250 μmol/L FeSO for 12 h and 24 h. Our data showed that higher iron intake resulted in higher hepatic iron without iron toxicity, and reduced body weight gain with no difference of food intakes. High dietary iron significantly increased 61% of hepatic glycogen deposition, but exhibited impairment in glucose responses in mice. Moreover, high dietary iron suppressed hepatic gluconeogenesis by repressing the expression of key gluconeogenic enzymes, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase. Meanwhile, mice fed with higher iron diets exhibited both decreased AMP-activated protein kinase (AMPK) activity and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) protein levels. Furthermore, in BRL-3A cells, iron treatment increased cellular glucose uptake, and altered gluconeogenesis rhythmically by regulating the activation of AMPK and expression of PGC-1α successively. This study demonstrated that dietary high iron was able to increase hepatic glycogen deposition by enhancement of glucose uptake, and suppress hepatic gluconeogenesis by regulation of AMPK and PGC-1α.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jnutbio.2022.109104DOI Listing
July 2022

WASH regulates the oxidative stress Nrf2/ARE pathway to inhibit proliferation and promote apoptosis of HeLa cells under the action of Jolkinolide B.

PeerJ 2022 13;10:e13499. Epub 2022 Jul 13.

Qiqihaer Medical University, Qiqihaer, China.

Jolkinolide B (JB), a diterpenoid compound isolated from the roots of , has gained research attention for its antitumor effects. In recent years, JB reportedly displayed anti-tumor activity in solid tumors, such as breast, ovarian, and prostate cancer, and leukemia. In this study, we evaluated the effect of JB on HeLa cells with a focus on cell growth inhibition and related mechanisms. HeLa cells were cultured and divided into a blank control group, HeLa-Scramble (0, 0.25, 0.5 mM), and Wiskott-Aldrich syndrome protein and SCAR homolog (WASH) protein silenced group, HeLa-shWASH (0, 0.25, 0.5 mM). Morphological changes were observed using an inverted microscope. The inhibition rate of cell proliferation was detected using the WST-1 method. Flow cytometry Brdu+PI double standard method was used to detect cell replication ability and FITC+PI double standard method was used to detect cell apoptosis rate. Western blot was used to verify the expression of Nrf2, HO-1, WASH, Bax, Bcl-2, and PCNA. The mRNA expression of cytokines (, , and ) was detected using RT-qPCR. The results showed that JB induced cell apoptosis and arrested cells at the G2/M phase in HeLa-shWASH cells compared with HeLa-Scramble cells ( < 0.05, < 0.01, respectively). In addition, JB upregulated , , and in HeLa-shWASH cells. We conclude that WASH protein participates in JB-induced regulation of the Nrf2/ARE pathway, aggravates inflammatory responses, and promotes cancer cell apoptosis, thus inhibiting the proliferation and invasion abilities of HeLa cells. JB may have anti-tumor effects and potential clinical value for the treatment of cervical cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.13499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288166PMC
July 2022

Microbial community composition and soil metabolism in the coexisting Cordyceps militaris and Ophiocordyceps highlandensis.

J Basic Microbiol 2022 Jul 19. Epub 2022 Jul 19.

Yunnan Herbal Laboratory, School of Ecology and Environmental Science, Yunnan University, Kunming, China.

Cordyceps sensu lato is a complex fungus-larva symbiote, and its distribution is affected by the geography, climate, soil environment, and cohabitating microorganisms. However, despite the fact Cordyceps militaris and Ophiocordyceps highlandensis are different species, they coexist in the Pinus armandi forest of Dashao in Songming County, Yunnan, China. We explored the microbial compositions and soil metabolism inhabited by C. militaris and O. highlandensis using high-throughput sequencing and nontargeted metabonomics. The results indicated that the bacterial and fungal compositions in the soil microhabitat communities of C. militaris, O. highlandensis, and null Cordyceps group were similar. However, the community compositions in the fruiting bodies of C. militaris and O. highlandensis were different. The dominant phylum Ascomycota and dominant genus Cordyceps were detected in the fruiting body of C. militaris. Except for Ascomycota, the dominant phylum Chytridiomycota and dominant genera Ophiocordyceps, Unclassified_k__Hypocreales, Circinotrichum, Cladosporium, and Unclassified_k__chytridiomycetesg were detected in the fruiting body of O. highlandensis. The plant probiotic bacteria Phyllobacterium was detected in the fruiting body of C. militaris. The growth-promoting bacteria Pseudomonas was detected in the fruiting body of O. highlandensis. Soil metabolism analysis revealed that pathways associated with amino acid metabolism was significantly enriched in O. highlandensis. Correlation analysis of bacterial diversity and soil metabolites revealed that the relative abundances of bacterial operational taxonomic units and the relative contents of metabolites were consistent. Our results provide insights into the microbial diversity and soil metabolism of naturally coexisting C. militaris and O. highlandensis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jobm.202200216DOI Listing
July 2022

[Bioinformatics analysis of IκB gene family expression in human lung adenocarcinoma].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2022 Aug;38(8):727-735

The School of Basic Medical Science, Southwest Medical University, Luzhou 646000, China.*Corresponding author, E-mail:

Objective To explore the clinical significance of inhibitor of NF-κB (IκB) family in lung adenocarcinoma (LUAD) through bioinformatics analysis. Methods The differentially expressed genes of IκB family in LUAD were screened by R language for survival analysis. The correlation between the expression of IκB family genes and clinicopathological characteristics was analyzed by R language, and the genes related to survival rate were selected for further study. GO and KEGG enrichment analyses were performed with LinkedOmics. The infiltration of immune cells was analyzed with TIMER. The correlation between the candidate genes and the prognosis of LUAD was analyzed through COX model. Results The expression levels of NF-κB inhibitor δ (NFKBID) and NF-κB inhibitor Zeta (NFKBIZ) were significantly downregulated in tumor tissues, and the patients with low expression levels of NFKBID and NFKBIZ had shorter overall survival. NFKBID and NFKBIZ were significantly correlated with T stage of LUAD. Enrichment analysis showed that low expression levels of NFKBID and NFKBIZ were correlated with energy metabolism and protein expression and transport. The expression levels of NFKBID and NFKBIZ were positively correlated with the infiltration of B cells, CD4 T cells, neutrophils, and dendritic cells. COX analysis indicated that NFKBIZ could be an independent prognostic factor for LUAD. Conclusion The expression levels of NFKBID and NFKBIZ were significantly downregulated in tumor tissues, and were correlated with overall survival. NFKBID and NFKBIZ could be involved in the occurrence and development of LUAD by regulating glycometabolism and multiple immune cells infiltration. NFKBIZ could be considered as an independent prognostic factor for LUAD.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2022

FB-EEGNet: A fusion neural network across multi-stimulus for SSVEP target detection.

J Neurosci Methods 2022 Sep 13;379:109674. Epub 2022 Jul 13.

College of Computer Science and Technology, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.

Background: Steady-state visual evoked potential (SSVEP) is a prevalent paradigm of brain-computer interface (BCI). Recently, deep neural networks (DNNs) have been employed for SSVEP target recognition. However, current DNN models can not fully extract information from SSVEP harmonic components, and ignore the influence of non-target stimuli.

New Method: To employ information of multiple sub-bands and non-target stimulus data, we propose a DNN model for SSVEP target detection, i.e., FB-EEGNet, which fuses features of multiple neural networks. Additionally, we design a multi-label for each sample and optimize the parameters of FB-EEGNet across multi-stimulus to incorporate the information from non-target stimuli.

Results: Under the subject-specific condition, FB-EEGNet achieves the average classification accuracies (information transfer rate (ITR)) of 76.75 % (50.70 bits/min) and 89.14 % (70.45 bits/min) in a time widow of 0.7 s under the public 12-target dataset and our experimental 9-target dataset, respectively. Under the cross-subject condition, FB-EEGNet achieved mean accuracies (ITRs) of 81.72 % (67.99 bits/min) and 92.15 % (76.12 bits/min) on the public and experimental datasets in a time window of 1 s, respectively.

Comparison With Existing Methods: FB-EEGNet shows superior performance than CCNN, EEGNet, CCA and FBCCA both for subject-dependent and subject-independent SSVEP target recognition.

Conclusion: FB-EEGNet can effectively extract information from multiple sub-bands and cross-stimulus targets, providing a promising way for extracting deep features in SSVEP using neural networks.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jneumeth.2022.109674DOI Listing
September 2022

Frequency-modulated continuous-wave 3D imaging with high photon efficiency.

Opt Lett 2022 Jul;47(14):3568-3571

Frequency-modulated continuous-wave (FMCW) light detection and ranging (LIDAR), which offers high depth resolution and immunity to environmental disturbances, has emerged as a strong candidate technology for active imaging applications. In general, hundreds of photons per pixel are required for accurate three-dimensional (3D) imaging. When it comes to the low-flux regime, however, depth estimation has limited robustness. To cope with this, we propose and demonstrate a photon-efficient approach for FMCW LIDAR. We first construct a FMCW LIDAR setup based on single-photon detectors where only a weak local oscillator is needed for the coherent detection. Further, to realize photon-efficient imaging, our approach borrows the data from neighboring pixels to enhance depth estimates, and employs a total-variation seminorm to smooth out the noise on the recovered depth map. Both simulation and experiment results show that our approach can produce high-quality 3D images from ∼10 signal photons per pixel, increasing the photon efficiency by 10-fold over the traditional processing method. The high photon efficiency will be valuable for low-power and rapid FMCW applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.463007DOI Listing
July 2022

HDL-C levels added to the MELD score improves 30-day mortality prediction in Asian patients with cirrhosis.

J Int Med Res 2022 Jul;50(7):3000605221109385

Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Objectives: Lower high-density lipoprotein cholesterol (HDL-C) levels have been observed in chronic liver disease patients. The aim of this study was to develop a model that incorporates HDL-C levels and the Model for End-stage Liver Disease (MELD) score to predict 30-day mortality in Asian cirrhosis patients.

Methods: Cirrhosis patients were recruited from two hospitals in this retrospective observational study. Propensity score matching was used. The model's performance was evaluated, including its ability to predict 30-day mortality, accuracy, and clinical utility. Net reclassification improvement (NRI) and integrated discrimination improvement (IDI) were calculated.

Results: The HDL-C + MELD model showed good ability to predict 30-day mortality (area under the curve, 0.784; sensitivity, 0.797; specificity, 0.632), which was better than that of the MELD score alone. It also showed good calibration and a net benefit for all patients, which was better than that of the MELD score, except at the threshold probability. NRI and IDI results confirmed that adding HDL-C levels to the MELD score improved the model's performance in predicting 30-day mortality.

Conclusion: We added HDL-C levels to the MELD score to predict 30-day mortality in Asian patients with cirrhosis. The HDLC + MELD model shows good ability to predict 30-day mortality and clinical utility.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/03000605221109385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9290124PMC
July 2022

Research on soft sensing method of photosynthetic bacteria fermentation process based on ant colony algorithm and least squares support vector machine.

Prep Biochem Biotechnol 2022 Jul 11:1-12. Epub 2022 Jul 11.

School of Electrical and Information Engineering, Jiangsu University, Zhenjiang, China.

Photosynthetic bacteria wastewater treatment is an efficient water pollution treatment method, but photosynthetic bacteria fermentation is a multivariable, non-linear, and time-varying process. So it is difficult to establish an accurate model. Aiming at the difficulty of online measurement of key parameters, such as bacterial concentration and matrix concentration in photosynthetic bacteria fermentation process, an improved ant colony algorithm least squares support vector machine (AC-LSSVM) soft sensing model method is proposed in this paper. Firstly, the virtual sensing subsystem of the photosynthetic bacteria fermentation process is proposed, with measurable parameters as input and unmeasurable key parameters as output, and the left inverse soft sensing model of virtual sensing is constructed. Then, the ant colony algorithm can quickly find the shortest path to optimize the parameters of the traditional PI regulation, to improve the dynamic performance and accuracy of parameter measurement in the fermentation process. After that, the ant colony algorithm is used to optimize penalty parameters C and kernel parameters σ of LSSVM, which effectively avoids the local optimization and improves the computing power and global optimization ability. Finally, the soft sensing prediction model of the photosynthetic bacteria fermentation process based on AC-LSSVM is established. Compared with SVM and LSSVM prediction models, the root mean square error of bacterial concentration and matrix concentration based on the AC-LSSVM model are 0.468 and 0.126, respectively. The simulation analysis shows that this model has less error and better prediction ability, and it can meet the needs of online prediction of key parameters of photosynthetic bacteria fermentation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10826068.2022.2090002DOI Listing
July 2022

The Influence of Long Working Hours, Occupational Stress, and Well-Being on Depression Among Couriers in Zhejiang, China.

Front Psychol 2022 23;13:928928. Epub 2022 Jun 23.

Occupational Health and Radiation Protection Institute, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.

Objective: This study aimed to examine the association between long working hours, occupational stress, depression, and well-being, and to explore the intermediary effect of occupational stress and well-being between working hours and depression among couriers in Zhejiang, China.

Methods: The study used a cluster random sampling method to select 1,200 couriers from mainstream express companies in Zhejiang, China. The data were collected and analyzed using the Core Occupational Stress Scale (COSS) to measure occupational stress, the Patient Health Questionnaire-9 (PHQ-9) scale to evaluate depression, and the World Health Organization five-item Well-Being Index (WHO-5) scale to assess well-being. Structural equation modeling (SEM) was used to test the hypothesized relationship among the variables.

Results: The phenomenon of long working hours (75.1%) was quite common among couriers in Zhejiang, China. Working hours had a direct positive effect on depression ( = 0.008,  < 0.001) and on occupational stress ( = 0.009,  < 0.001), and working hours had a negative effect on well-being ( = -0.013,  < 0.001). Occupational stress had a direct positive effect on depression ( = 0.272,  < 0.001), but well-being had no significant direct effect on depression. Working hours had an indirect effect on depression through the mediating effect of occupational stress while the mediating effect of well-being was not found.

Conclusion: Long working hours is associated with occupational stress, well-being, and depression. Our results confirmed that working hours, occupational stress, and well-being were strong predicators of depression. Working hours had a significant indirect effect on depression occupational stress. The result of this study showed that decreasing working hours and reducing occupational stress would be effective for couriers to prevent depression. However, more studies are needed to verify the relationship between working hours and depression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2022.928928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260285PMC
June 2022

Icariside II suppresses the tumorigenesis and development of ovarian cancer by regulating miR-144-3p/IGF2R axis.

Drug Dev Res 2022 Jul 9. Epub 2022 Jul 9.

Department of Pathology, Taizhou People's Hospital, Affiliated Hospital of NanJing Medical University, Taizhou, Jiangsu, China.

Ovarian cancer is one of the three major gynecological malignancies. It has been reported that Icariside II was able to block the occurrence and development of ovarian cancer. However, the detailed mechanism by which Icariside II regulates the development of ovarian cancer is widely unknown. EdU staining and transwell assays were applied to detect the proliferation, migration, and invasion of ovarian cancer cells. Next, the relationship between miR-144-3p and IGF2R was verified by the dual-luciferase reporter assay. Moreover, in vivo animal model was constructed to verify the effect of Icariside II on the development of ovarian cancer. Icariside II notably inhibited the proliferation, migration, and invasion and induced the apoptosis of ovarian cancer cells. Additionally, Icariside II markedly increased the level of miR-144-3p in ovarian cancer cells. Moreover, IGF2R was targeted by miR-144-3p directly. Icariside II significantly decreased the expression of IGF2R and the phosphorylation level of AKT and mTOR in ovarian cancer cells, which were partially reversed by miR-144-3p inhibitor. Meanwhile, Icariside II remarkably promoted the autophagy of ovarian cancer cells, as confirmed by the increased expression of Beclin-1 and ATG-5 and decreased expression of p62; however, co-treatment with miR-144-3p inhibitor notably decreased autophagy. Furthermore, the result of animal study suggested Icariside II notably inhibited ovarian tumor growth as well. Collectively, Icariside II could suppress the tumorigenesis and development of ovarian cancer by promoting autophagy via miR-144-3p/IGF2R axis. These results may be beneficial for future studies on the use of Icariside II to treat ovarian cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ddr.21967DOI Listing
July 2022

Some Preliminary Results to Eradicate Leukemic Cells in Extracorporeal Circulation by Actuating Doxorubicin-Loaded Nanochains of FeO Nanoparticles.

Cells 2022 Jun 23;11(13). Epub 2022 Jun 23.

The State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Sciences and Medical Engineering, Southeast University, Dingjiaqiao 87, Nanjing 210009, China.

Leukemia is a non-solid cancer which features the malignant proliferation of leukocytes. Excessive leukocytes of lesions in peripheral blood will infiltrate organs, resulting in intumescence and weakening treatment efficiency. In this study, we proposed a novel approach for targeted clearance of the leukocytes in the peripheral blood ex vivo, which employed magnetic nanochains to selectively destroy the leukocytes of the lesions. The nanochains were doxorubicin-loaded nanochains of FeO nanoparticles which were fabricated by the solvent exchange method combined with magnetic field-directed self-assembly. Firstly, the nanochains were added into the peripheral blood during extracorporeal circulation and subjected to a rotational magnetic field for actuation. The leukocytes of the lesion were then conjugated by the nanochains via folic acid (FA) targeting. Finally, the rotational magnetic field actuated the nanochains to release the drugs and effectively damage the cytomembrane of the leukocytes. This strategy was conceptually shown in vitro (K562 cell line) and the method's safety was evaluated in a rat model. The preliminary results demonstrate that the nanochains are biocompatible and suitable as drug carriers, showing direct lethal action to the leukemic cells combined with a rotational magnetic field. More importantly to note is that the nanochains can be effectively kept from entry into the body. We believe this extracorporeal circulation-based strategy by activating nanochains magnetically could serve as a potential method for leukemia treatment in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cells11132007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265363PMC
June 2022

OsMPK4 promotes phosphorylation and degradation of IPA1 in response to salt stress to confer salt tolerance in rice.

J Genet Genomics 2022 Jul 5. Epub 2022 Jul 5.

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Salt stress adversely affects plant growth, development, and crop yield. Rice (Oryza sativa L.) is one of the most salt-sensitive cereal crops, especially at the early seedling stage. Mitogen-activated protein kinase (MAPK/MPK) cascades have been shown to play critical roles in salt response in Arabidopsis. However, the roles of the MPK cascade signaling in rice salt response and substrates of OsMPK remain largely unknown. Here, we report that the salt-induced OsMPK4-Ideal Plant Architecture 1 (IPA1) signaling pathway regulates the salt tolerance in rice. Under salt stress, OsMPK4 could interact with IPA1 and phosphorylate IPA1 at Thr180, leading to degradation of IPA1. Genetic evidence shows that IPA1 is a negative regulator of salt tolerance in rice, whereas OsMPK4 promotes salt response in an IPA1-dependent manner. Taken together, our results uncover an OsMPK4-IPA1 signal cascade that modulates the salt stress response in rice and sheds new light on the breeding of salt-tolerant rice varieties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jgg.2022.06.009DOI Listing
July 2022

Improving the efficiency of prime editing with epegRNAs and high-temperature treatment in rice.

Sci China Life Sci 2022 Jun 23. Epub 2022 Jun 23.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310006, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11427-022-2147-2DOI Listing
June 2022

Transport properties of MoS/V(Bz) and graphene/V(Bz) vdW junctions tuned by bias and gate voltages.

RSC Adv 2022 Jun 13;12(27):17422-17433. Epub 2022 Jun 13.

HeiLongJiang Construction Investment Group Co. Ltd No. 523, Sanda Dongli Road Harbin 150040 P. R. China.

The MoS/V(Bz) and graphene/V(Bz) vdW junctions are designed and the transport properties of their four-terminal devices are comparatively investigated based on density functional theory (DFT) and the nonequilibrium Green's function (NEGF) technique. The MoS and graphene nanoribbons act as the source-to-drain channel and the spin-polarized one-dimensional (1D) benzene-V multidecker complex nanowire (V(Bz)) serves as the gate channel. Gate voltages applied on V(Bz) exert different influences of electron transport on MoS/V(Bz) and graphene/V(Bz). In MoS/V(Bz), the interplay of source and gate bias potentials could induce promising properties such as negative differential resistance (NDR) behavior, output/input current switching, and spin-polarized currents. In contrast, the gate bias plays an insignificant effect on the transport along graphene in graphene/V(Bz). This dissimilarity is attributed to the fact that the conductivity follows the sequence of MoS < V(Bz) < graphene. These transport characteristics are examined by analyzing the conductivity, the currents, the local density of states (LDOS), and the transmission spectra. These results are valuable in designing multi-terminal nanoelectronic devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2ra02196jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189623PMC
June 2022

An expanded access protocol of RT001 in amyotrophic lateral sclerosis---Initial experience with a lipid peroxidation inhibitor.

Muscle Nerve 2022 Jun 28. Epub 2022 Jun 28.

Sean M. Healey and AMG Center for ALS & the Neurological Clinical Research Institute, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.

Introduction/aims: Lipid peroxidation is thought to play a biologically important role in motor neuron death in amyotrophic lateral sclerosis (ALS). 11,11 Di-deuterated linoleic ethyl ester (RT001) prevents lipid peroxidation in cellular and mitochondrial membranes. Herein we report on the use of RT001 under expanded access (EA).

Methods: We provided RT001 to patients with ALS via EA at a single site. The starting dose was 2.88 g/day, which was increased to to 8.64 g/day as tolerated. Participants were not eligible for alternative clinical trials. Participants were followed for adverse events and pharmacokinetic (PK) parameters were measured approximately 3 months after RT001 initiation.

Results: Sixteen participants received RT001 (5.6 ± 1.6 g/day; dose range, 1.92 to 8.64 g/day) for a mean period of 10.8 ± 7.1 months. After 3 months of treatment, PK studies showed that RT001 was absorbed, metabolized, and incorporated into red blood cell membranes at concentrations expected to be therapeutic based on in vitro models. The most common adverse events were gastrointestinal, including diarrhea, which occurred in 25% of the participants, and were considered possibly related to RT001. One participant (6%) discontinued due to an adverse event. Ten serious adverse events occurred: these events were recognized complications of ALS and none were attributed to treatment with RT001.

Discussion: RT001 was administered safely to a small group of people living with ALS in the context of an EA protocol. Currently, there is an ongoing randomized, double-blind, controlled study of RT001 in ALS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mus.27672DOI Listing
June 2022

A novel irinotecan derivative ZBH-1207 with different anti-tumor mechanism from CPT-11 against colon cancer cells.

Mol Biol Rep 2022 Jun 29. Epub 2022 Jun 29.

Cell Biology Laboratory, Jilin Province Tumor Institute, Jilin Cancer Hospital, No.1018 Hu-Guang Road, Changchun, 130012, China.

Purpose: Irinotecan (CPT-11) is a camptothecin derivative whose potent anti-tumor activity depends on the rapid formation of an in vivo active metabolite, SN38 (7-ethyl-10-hydroxycamptothecin). CPT-11 combine with other agents are often the treatment of choice for patients with advanced or metastatic colorectal cancer (CRC). This study evaluates the cytotoxic mechanism of a novel CPT-11 derivative, ZBH-1207 in CRC cells in vitro.

Methods: The anti-proliferation effect of ZBH-1207 on tumor cells was assessed by MTT assay. The inhibition of TOP1, the alteration of cell cycle and apoptosis, and the expression of caspase-3 and PARP in CRC cells induced by ZBH-1207 were detected by DNA relaxation assay, flow cytometry, and Western blot, respectively.

Results: ZBH-1207 significantly inhibits the proliferation of seven tumor cell lines and retains the activity of TOP1 as compared with CPT-11. Treatment with ZBH-1207 results in more apparent cell cycle arrests and apoptosis of CRC cells than that of CPT-11 and SN38. Accordingly, up-regulation of active caspase-3 and PARP expression were relatively higher in ZBH-1207 group than that in CPT-11 and SN38 group.

Conclusion: ZBH-1207 has higher cytotoxicity than CPT-11/SN38 in CRC cells. Its molecular mechanism involves apoptosis signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-022-07652-2DOI Listing
June 2022
-->