Publications by authors named "Hong Yao"

523 Publications

Investigation of properties change in the reacted molten salts after molten chlorides cyclic thermal treatment of toxic MSWI fly ash.

J Hazard Mater 2021 Jul 16;421:126536. Epub 2021 Jul 16.

State Key Laboratory of Coal Combustion, School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

To realize the thermal detoxification of municipal solid waste incineration (MSWI) fly ash in a relatively mild environment, molten salts thermal treatment technology was proposed in our previous research, which showed good effects. To investigate the properties of molten salts (NaCl-CaCl) during cycling reusing, the change of the main components and the physical properties of the used molten salts were estimated. Results showed that the salts in fly ash would dissolve into molten salts. During this process, the concentration of K, SO kept increasing while Cl was decreased. The changing trend of Na and Ca was dependent on the ratio of Ca/Na in raw fly ash. Ca(OH) in fly ash would react with CaCl to form CaClOH. Moreover, the introduction of the salt components on the thermal properties of molten salts were also studied. The melting point hardly changed by NaCl, CaSO, and SiO. Nevertheless, it was lowered to 431 °C with 15% CaCO addition, while increased to 523 °C with 20% KCl. Besides, there were no significant influences on the viscosity, stability, and thermal diffusivity of molten salts. KCl had the greatest influence on the specific heat capacity of molten salt, with an increase of about 20%.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126536DOI Listing
July 2021

Hdpa derivatives containing pentadentate ligands: An acyclic adjuvant potentiates meropenem activity in vitro and in vivo against metallo-β-lactamase-producing Enterobacterales.

Eur J Med Chem 2021 Jul 13;224:113702. Epub 2021 Jul 13.

School of Pharmaceutical Sciences, Institute of Drug Discovery and Development, Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, PR China. Electronic address:

The emergence and dissemination of metallo-β-lactamases (MBLs) producing Enterobacterales is a great concern for public health due to the limited therapeutic options. No MBL inhibitors are currently available in clinical practice. Herein, we synthesized a series of Hdpa derivatives containing pentadentate-chelating ligands and evaluated their inhibitory activity against MBLs. Related compounds inhibited clinically relevant MBLs (Imipenemase, New Delhi metallo-β-lactamase (NDM) and Verona integron-encoded metallo-β-lactamase) with IC values of 1-4.9 μM. In vitro, the most promising compounds, 5b and 5c, which had a chiral methyl at the acid adjacent to 5a, demonstrated potent synergistic activity against engineered strains, with fractional inhibitory concentration index values as low as 0.07-0.18. The addition of 5b and 5c restored meropenem efficacy against 42 MBL-producing Enterobacterales and Pseudomonas aeruginosa to satisfactory clinical levels. In addition, safety tests revealed that 5b/5c showed no toxicity in red blood cells, cell lines or mouse model. Further studies demonstrated that compounds 5b and 5c were non-competitive MBL inhibitors. In vivo compounds 5b and 5c potentiated meropenem efficacy and increased the survival rate from 0 to at least 83% in mice with sepsis caused by an NDM-1-positive clinical strain. The activity of the compounds exhibited consistency at the molecular, cellular, and in vivo levels. These data indicated that Hdpa derivatives 5b and 5c containing pentadentate-chelating ligands may be worthy of further study.
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http://dx.doi.org/10.1016/j.ejmech.2021.113702DOI Listing
July 2021

Torrefaction under mechanical pressure of 10-70 MPa at 250 °C and its effect on pyrolysis behaviours of leucaena wood.

Bioresour Technol 2021 Jul 5;338:125503. Epub 2021 Jul 5.

The Joint Graduate School of Energy and Environment, Center of Excellence on Energy Technology and Environment, King Mongkut's University of Technology Thonburi, Bangmod, Tungkru, Bangkok 10140, Thailand. Electronic address:

In this study, torrefaction under mechanical pressure of 10-70 MPa at 250 °C was proposed as a pretreatment method and its effect on pyrolysis behaviours of Leucaena (LC) was examined at 900 °C. It was found that the mechanical pressure applied during torrefaction could significantly increase the char yield at 900 °C. The char yield increased from 18.7% for Raw to 26.4% and 27.5% for MP40 and MP70, respectively. The %C of biochar prepared from MP40 (MP40-900) was 86.5%, whereas the %C of biochar prepared from raw (Raw-900) was 82.6%. From TG-MS analyses during the pyrolysis of MP, a large amount of oxygen was removed as HO and CO. The analyses of tars produced from MP showed higher fraction of acids and furans compared with tar produced from Raw. Furthermore, the mechanism of the pyrolysis of LC torrefied under mechanical pressure was discussed.
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http://dx.doi.org/10.1016/j.biortech.2021.125503DOI Listing
July 2021

The effects of Fe(III) and Fe(II) on anammox process and the Fe-N metabolism.

Chemosphere 2021 Jun 25;285:131322. Epub 2021 Jun 25.

Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, Department of Municipal and Environmental Engineering, School of Civil Engineering, Beijing Jiaotong University, Beijing, 100044, PR China.

This study aims to compare the effects of different Fe stress on anammox (anaerobic ammonium oxidation) process, therefore seven identical reactors were operated under different Fe(II)/Fe(III) concentrations. After 38 days of operation, the anammox activity was highest (10.49 ± 0.41 mg-TN/(g-VSS·h)) under conditions of 5 mg/L-Fe(II), while under 30 mg/L-Fe(III) displayed severe inhibition. The results showed that continuous addition of 30 mg/L-Fe(III) would damage the composition of EPS (extracellular polymeric substances) and make anammox bacteria more sensitive to environmental stress. While high Fe(II) concentrations could result in precipitates encasing granular sludge, affecting substrate utilization. Moreover, the results of ΔNO-N/ΔNH-N indicated that Fe(II)-dependent nitrate reduction was induced in reactors added with Fe(II). OM27_clade and norank_f__Burkholderiaceae might be candidates for this process according to the correlation of genera and functional genes (based on the PICRUSt 2 functional prediction). Overall, this research is expected to provide new ideas to the effects of Fe(II)/Fe(III) on anammox and to the practical application of coupled system based on anammox in wastewater treatment.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131322DOI Listing
June 2021

Sessile methanogens dominated cathodic biofilm: Distribution and network in physiological transitions.

Sci Total Environ 2021 Jul 1;795:148724. Epub 2021 Jul 1.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China; Shenzhen Key Laboratory of Organic Pollution Prevention and Control, Environmental Science and Engineering Research Center, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China; CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

A mature cathodic biofilm plays the key role in biocathode electron transfer. The physiological transitions of it were of great interests as the sessile and dispersed (planktonic) work in a balance while it is still lack of understanding. In this study, an improved sampling method was used for detecting sessile, detached, and dispersed microorganisms. The results indicated a certain number of methanogens (82.6%) and its associated phyla (60%-90%) are immobilized as sessile biofilm. The Tax4Fun predicted a lower abundance of mobility associated genes and a significant enrichment (t-test, P = 0.003) of c-di-GMP in sessile biofilm. Overall, the microbial interaction and motility were predicted as two factors to affect the physiological transitions of cathodic biofilm. This finding could shed a light on the investigation of cathodic biofilm in a dynamic transition rather than a static community, playing a pivotal role in understanding the relation between specific property of biofilm.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148724DOI Listing
July 2021

Cultivation of aerobic granular sludge coupled with built-in biochemical cycle galvanic-cells driven by dual selective pressure and its denitrification characteristics.

Bioresour Technol 2021 Oct 24;337:125454. Epub 2021 Jun 24.

School of Civil Engineering, Beijing Jiaotong University, Beijing 100044, PR China; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, Beijing 100044, PR China.

Dual selective pressure was applied as the driving condition to cultivate an enhanced aerobic granular sludge (AGS) with Fe(0)-based biochemical cycle galvanic-cells (BCGC) as the core. The BCGC-AGS coupled micro-electrolysis with synergistic autotrophic-heterotrophic denitrification to enhance nitrogen removal. COD and total nitrogen removal of 91.8% and 95.9% were achieved, respectively. The formation of circulation channel between Fe and Fe provided a solid foundation for the biochemical cycle of galvanic-cells with low consumption. The existence of micro-electrolysis selective pressure in BCGC-AGS was also confirmed. Facultative aerobic bacteria Methylocystis and Azospirillum were the most abundant genera. Facultative iron redox bacteria and autotrophic denitrifying bacteria Geobacter, Thiobacillus, Aquabacterium, Thauera and Azospirillum showed high abundance, affirming the success culture of EAGS system. Load shock test verified BCGC-AGS possessed excellent load shock resistance. Obtaining the advantages of fast-cultivation, high-efficiency and low galvanic-cells consumption, BCGC-AGS showed significant potential for practical application.
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http://dx.doi.org/10.1016/j.biortech.2021.125454DOI Listing
October 2021

Photocatalytically reductive defluorination of perfluorooctanoic acid (PFOA) using Pt/LaTiO nanoplates: Experimental and DFT assessment.

J Hazard Mater 2021 Jun 24;419:126452. Epub 2021 Jun 24.

John A. Reif, Jr. Department of Civil and Environmental Engineering, New Jersey Institute of Technology, Newark, NJ 07102, USA; Otto H. York Department of Chemical and Materials Engineering, New Jersey Institute of Technology, Newark, NJ 07102, USA. Electronic address:

To enable efficient degradation of perfluorooctanoic acid (PFOA), we prepared and investigated a nanosheet photocatalyst (Pt/LaTiO). Doping platinum nanoparticles on LaTiO varied the band structure and improved the photocatalytic activity due to the enhanced charge separation. Methanol as the electron donor and sacrificial reagent significantly promoted the photocatalytically reductive degradation of PFOA that reduced by 40% within 180 min under UV irradiation (1 mW∙cm). The density functional theory calculations were used to analyze the effects of Pt doping and band structures on interfacial electron transfer and degradation pathways. As opposed to photocatalytic oxidation, this UV/Pt/LaTiO/CHOH photocatalysis could abstract electrons from methanol and convert to free reactive radicals and photo-generated electrons to reductively defluorinate PFOA and potentially other polyhalogenated or perhalogenated organic compounds.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126452DOI Listing
June 2021

Development of Alectinib-Based PROTACs as Novel Potent Degraders of Anaplastic Lymphoma Kinase (ALK).

J Med Chem 2021 Jul 28;64(13):9120-9140. Epub 2021 Jun 28.

State Key Laboratory of Natural Medicines and Department of Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, P. R. China.

A series of novel anaplastic lymphoma kinase (ALK) degraders were designed and synthesized based on proteolysis-targeting chimera (PROTAC) technology by linking two alectinib analogs ( and ) with pomalidomide through linkers of different lengths and types. The most promising degrader possessed a high ALK-binding affinity and potent antiproliferative activity in the ALK-dependent cell lines and did not exhibit obvious cytotoxicity in ALK fusion-negative cells. More importantly, the efficacy of compound in a Karpas 299 xenograft mouse model was further evaluated based on its ALK-sustained degradation ability . The reduction in tumor weight in the compound -treated group (10 mg/kg/day, I.V.) reached 75.82%, while alectinib reduced tumor weight by 63.82% at a dose of 20 mg/kg/day (P.O.). Taken together, our findings suggest that alectinib-based PROTACs associated with the degradation of ALK may have promising beneficial effects for treating ALK-driven malignancies.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00270DOI Listing
July 2021

Performance and microbial community dynamics relationship within a step-feed anoxic/oxic/anoxic/oxic process (SF-A/O/A/O) for coking wastewater treatment.

Sci Total Environ 2021 Jun 4;792:148263. Epub 2021 Jun 4.

Beijing International Scientific and Technological Cooperation Base of Water Pollution Control Techniques for Antibiotics and Resistance Genes, Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Civil Engineering, Beijing Jiaotong University, Beijing 100044, PR China.

A step-feed anoxic/oxic/anoxic/oxic (SF-A/O/A/O) was developed and successfully applied to full-scale coking wastewater treatment. The performance and microbial community were evaluated and systematically compared with the anoxic/oxic/oxic (A/O/O) process. SF-A/OA/O process exhibited efficient removal of COD, NH-N, TN, phenols, and cyanide with corresponding average effluent concentrations of 317.9, 1.8, 46.2, 1.1, and 0.2 mg·L, respectively. In particular, the TN removal efficiency of A/O/O process was only 7.8%, with an effluent concentration of 300.6 mg·L. Furthermore, polycyclic aromatic hydrocarbons with high molecular weight were the dominant compounds in raw coking wastewater, which were degraded to a greater extent in SF-A/OA/O. The abundance in Thiobacillus, SM1A02, and Thauera could be the main reason why SF-A/O/A/O was superior to A/O/O in treating TN. The microbial community structure of SF-A/O/A/O was similar among stages in system (P ≥ 0.05, Welch's t-test) and was less affected by environmental factors, which may have been one of the important factors in the system's strong stability.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148263DOI Listing
June 2021

Synergistic adsorption and degradation of sulfamethoxazole from synthetic urine by hickory-sawdust-derived biochar: The critical role of the aromatic structure.

J Hazard Mater 2021 Jun 9;418:126366. Epub 2021 Jun 9.

Department of Civil Engineering, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA; Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA. Electronic address:

This study investigated the adsorptive removal and subsequent degradation of sulfamethoxazole (SMX) from a synthetic urine by biochar (BC). The BCs used in this study were prepared using two different feedstocks with different temperatures. Element analysis and Fourier transform infrared spectroscopy (FTIR) results suggested that the aromaticity of one of the BCs, 700HSBC was significantly different from the 700PSBC although both of them were prepared at the same temperature (700 °C) with similar pore size distributions and specific surface areas. Due to the presence of abundant aromatic structures, 700HSBC showed a higher SMX uptake than 700PSBC, suggesting that the π-π interaction was the main adsorption mechanism. The removal of SMX from the urine was significantly enhanced by adding hydrogen peroxide to the 700HSBC. The carbonate radicals degradation of SMX mechanism was proposed and verified. With 700HSBC having abundant aromatic structures acting as π-electron donors, it could be an efficient activator for peroxymonocarbonate (HCO) to generate carbonate radicals. Hence, it could be concluded that the aromatic structures on BCs play a key role in both of the adsorption and hydrogen peroxide degradation of the SMX resulting in its removal from urine.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126366DOI Listing
June 2021

Experimental Evidence of Chiral Symmetry Breaking in Kekulé-Ordered Graphene.

Phys Rev Lett 2021 May;126(20):206804

State Key Laboratory of Low-Dimensional Quantum Physics and Department of Physics, Tsinghua University, Beijing 100084, People's Republic of China.

The low-energy excitations of graphene are relativistic massless Dirac fermions with opposite chiralities at valleys K and K^{'}. Breaking the chiral symmetry could lead to gap opening in analogy to dynamical mass generation in particle physics. Here we report direct experimental evidences of chiral symmetry breaking (CSB) from both microscopic and spectroscopic measurements in a Li-intercalated graphene. The CSB is evidenced by gap opening at the Dirac point, Kekulé-O type modulation, and chirality mixing near the gap edge. Our work opens up opportunities for investigating CSB related physics in a Kekulé-ordered graphene.
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http://dx.doi.org/10.1103/PhysRevLett.126.206804DOI Listing
May 2021

Developing a Subjective Evaluation Scale for Assessing the Built Environments of China's Hygienic City Initiative.

Biomed Environ Sci 2021 May;34(5):372-378

Office of Epidemiology, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Objective: To develop a preliminary subjective evaluation scale for assessing the built environments of China's Hygienic City Initiative and to evaluate its reliability and validity.

Methods: The initial items of the scale were determined based on a review of policy documents and consultations with experts. The final items of the scale were confirmed through individual interviews with residents combined with the discretetrend method, critical ratio method, correlation coefficient method, and factor analysis method. Then, the dimensions of the scale were determined using exploratory factor analysis (EFA). The Cronbach's coefficient, split-half reliability coefficient, and confirmatory factor analysis (CFA) were used to assess the reliability and validity of the scale.

Results: A scale containing five dimensions with 22 items was established, including urban lifestyle, governance, basic functions, environmental sanitation, and amenities. The Cronbach's coefficient of the scale was 0.876, and the split-half reliability coefficient was 0.796. The CFA results indicate that each inspection level was within the standard limit.

Conclusion: The preliminarily subjective evaluation scale for assessing the built environments of China's Hygienic City Initiative demonstrates a high level of reliability and validity. Additional empirical studies should be carried out to further verify the value of the scale in terms of practical application.
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http://dx.doi.org/10.3967/bes2021.049DOI Listing
May 2021

Increases in Height among Chinese Children and Adolescents by Gender: An Age-Period-Cohort Analysis.

Biomed Environ Sci 2021 May;34(5):348-355

Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Beihang University & Capital Medical University, Beijing 100045, China;Big Data and Engineering Research Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.

Objective: To examine increases in average height among Chinese children and adolescents.

Methods: The data were obtained from the China Health and Nutrition Survey conducted during the period 1989-2015. A stratified multistage cluster sampling method was utilized to select participants aged 2-22 years in each province. Linear regression was used to examine the effects of age, birth cohort, and survey period on height.

Results: A total of 15,227 males and 13,737 females were included in the final analysis. Age (A) showed a continuous effect on height. The average heights of the investigated groups increased continuously during the investigation period. By 2015, the average height of the overall group increased by 7.87 cm compared to the average height during the 1989 survey. Moreover, birth year (cohort, C) also had a stable effect on height. Using the height of individuals born in or before 1975 as a reference, the average height of each birth cohort increased in comparison to the previous birth cohort.

Conclusions: The height of Chinese children and adolescents was affected by age, period, and cohort effects, and this effect is governed by certain rules. The age-period-cohort model can be used to analyze the trends of children's and adolescent's heights. The findings provide a scientific basis for the formulation of children's and adolescents' growth and development policies in China.
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http://dx.doi.org/10.3967/bes2021.046DOI Listing
May 2021

Discovery of Novel Tacrine-Pyrimidone Hybrids as Potent Dual AChE/GSK-3 Inhibitors for the Treatment of Alzheimer's Disease.

J Med Chem 2021 06 23;64(11):7483-7506. Epub 2021 May 23.

State Key Laboratory of Natural Medicines and Department of Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.

Based on a multitarget strategy, a series of novel tacrine-pyrimidone hybrids were identified for the potential treatment of Alzheimer's disease (AD). Biological evaluation results demonstrated that these hybrids exhibited significant inhibitory activities toward acetylcholinesterase (AChE) and glycogen synthase kinase 3 (GSK-3). The optimal compound possessed excellent dual AChE/GSK-3 inhibition both in terms of potency and equilibrium (AChE: IC = 51.1 nM; GSK-3β: IC = 89.3 nM) and displayed significant amelioration on cognitive deficits in scopolamine-induced amnesia mice and efficient reduction against phosphorylation of tau protein on Ser-199 and Ser-396 sites in glyceraldehyde (GA)-stimulated differentiated SH-SY5Y cells. Furthermore, compound exhibited eligible pharmacokinetic properties, good kinase selectivity, and moderate neuroprotection against GA-induced reduction in cell viability and neurite damage in SH-SY5Y-derived neurons. The multifunctional profiles of compound suggest that it deserves further investigation as a promising lead for the prospective treatment of AD.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00160DOI Listing
June 2021

A comparative study for phosphate adsorption on amorphous FeOOH and goethite (α-FeOOH): An investigation of relationship between the surface chemistry and structure.

Environ Res 2021 08 13;199:111223. Epub 2021 May 13.

Department of Civil Engineering, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA. Electronic address:

Eutrophication is generally caused by excess nitrogen and phosphorus being released into surface waters by runoff. Developing adsorbents for adsorbing phosphate within soil buffer zones and/or water treatment columns may be effective methods to mitigate this problem. In this study, an amorphous FeOOH (AF) and a well-crystallized α-FeOOH (CF) was formulated to compare phosphate adsorption behavior. The physicochemical properties between these species showed significant differences in morphology, crystallization, zeta potential, and specific surface area. The AF exhibited higher phosphate uptake than CF. X-ray photoelectron spectroscopy (XPS) verified that the hydroxyl groups within AF were 13.28% higher than that in CF. The triply coordinated hydroxyl groups (μ-OH) associated with AF and CF appeared at different positions as shown in the diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) analyses, confirming that AF contains more adsorption reactive sites (μ-OH). Mechanisms for monodentate formations and a stable six-member ring structure were proposed. The X-ray absorption near the edge structure (XANES) and XPS results suggested that the iron valence in AF was dominated by Fe (III). XANES also demonstrated that the amorphous structure found in the AF was caused by the disordered tetrahedron and octahedron alignments, leading to a higher phosphate adsorption.
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http://dx.doi.org/10.1016/j.envres.2021.111223DOI Listing
August 2021

formation of Hg-coordinated fluorescent nanoparticles through a supramolecular polymer network used for efficient Hg sensing and separation.

Nanoscale 2021 May;13(20):9172-9176

College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, P. R. China.

There have been many new methods for synthesizing novel nanomaterials with unique functions. Herein, a novel strategy to form fluorescent nanoparticles in situ has been developed, and it can be applied to efficiently sense Hg2+ in living cells and also separate Hg2+ from water.
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http://dx.doi.org/10.1039/d1nr01599kDOI Listing
May 2021

Estimating metal loading into the sea from tidal rivers using total suspended solids and water quality models.

Integr Environ Assess Manag 2021 May 12. Epub 2021 May 12.

School of Geographic and Oceanographic Science, Nanjing University, Nanjing, China.

Metals are mainly transported with suspended sediments in tidal rivers, and it is difficult to describe the transformation process of metals. Freely dissolved metals and suspended metals (including acid-soluble, reducible, oxidizable, and residual metals) were separately analyzed according to a sequential extraction scheme. The regression of the observed suspended metals and total suspended solids (TSS) concentrations allowed us to couple the two pollutant types. TSS is a highly dynamic parameter, and a model can be developed to simulate TSS transport. Thus, a method including the following five steps was proposed to estimate the loadings of metals entering the sea using TSS concentrations and water quality models: (1) collecting and observing basic information on the hydrological conditions, pollution sources, and water quality of the studied river; (2) obtaining a regression model between the suspended metals and TSS and analyzing the potential of establishing a suspended metal flux model; (3) introducing a model describing the fluxes of water-soluble metals into the sea; (4) establishing a calculation model to determine the fluxes of suspended metals into the sea; and (5) characterizing the fluxes of metals into the sea. The method was programmed, and metal concentrations and fluxes could be characterized quickly when the basic river data were sufficient. In addition, if regional development scenarios could be set, metal loadings in all scenarios could be predicted through the procedure developed herein, and some effective suggestions on regional sustainable development might be proposed for decision makers. Integr Environ Assess Manag 2021;00:1-11. © 2021 SETAC.
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http://dx.doi.org/10.1002/ieam.4440DOI Listing
May 2021

An assessment of the analytical performance of non-invasive prenatal testing (NIPT) in detecting sex chromosome aneuploidies: 34,717-patient sample in a single prenatal diagnosis Centre in China.

J Gene Med 2021 May 11:e3362. Epub 2021 May 11.

Department of Gynecology & Obstetrics, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Objective: The present study aimed to evaluate the efficacy of a non-invasive prenatal test (NIPT) in the detection of the sex chromosome aneuploidies (SCAs) at our prenatal diagnosis centre.

Methods: Among a cohort of 34,717 pregnancies, maternal plasma samples from our prenatal diagnosis centre were subject to analysis of SCAs using NIPT detection. Pregnant women with NIPT positive results of SCAs were recommended to undergo an invasive prenatal diagnosis (i.e. karyotyping and fluorescence in situ hybridization) to validate the prediction value of NIPT.

Results: From 34,717 clinical pregnancies, 229 (0.66%) pregnancies were identified with SCAs. Of these, 78 (34.1%) cases were positive for 45,X and 151 (65.9%) cases comprised a sex chromosome trisomy. Of the 229 positive NIPT results, 193 (84.3%) cases had accepted an invasive diagnosis involving karyotyping analysis of the amniotic fluid, which confirmed 67 cases (34.7%) as true positive, as well as 126 cases (65.3%) as false positive. The positive predictive values were 23.07%, 50%, 36% and 27.27% respectively. The remaining 36 (15.7%) cases declined a prenatal diagnosis. The termination rates of 45,X, 47,XXY, 47,XXX and 47,XYY were 20.5%,46%,12.9% and 11.5% respectively.

Conclusions: NIPT demonstrated a lower accuracy in predicting monosomy X than sex chromosome trisomies. After invasive testing, the fetal chromosome with 45,X and 47,XXY were terminated more often than those with 47,XXX, 47,XYY. Because NIPT is a screening test, false positive/negative cases exist, and pre- and post-test counselling is essential for informing patients about the benefits and limitations of the test. Confirmatory testing of abnormal results is recommended prenatally or after birth, and the importance of confirmatory testing and benefits of early diagnosis should be addressed.
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http://dx.doi.org/10.1002/jgm.3362DOI Listing
May 2021

Formic Acid as a Potential On-Board Hydrogen Storage Method: Development of Homogeneous Noble Metal Catalysts for Dehydrogenation Reactions.

ChemSusChem 2021 Jul 7;14(13):2655-2681. Epub 2021 Jun 7.

School of Metallurgy and Environment, Central South University, 932 Lushan Road, Changsha city, Hunan Province, 410083, P. R. China.

Hydrogen can be used as an energy carrier for renewable energy to overcome the deficiency of its intrinsically intermittent supply. One of the most promising application of hydrogen energy is on-board hydrogen fuel cells. However, the lack of a safe, efficient, convenient, and low-cost storage and transportation method for hydrogen limits their application. The feasibility of mainstream hydrogen storage techniques for application in vehicles is briefly discussed in this Review. Formic acid (FA), which can reversibly be converted into hydrogen and carbon dioxide through catalysis, has significant potential for practical application. Historic developments and recent examples of homogeneous noble metal catalysts for FA dehydrogenation are covered, and the catalysts are classified based on their ligand types. The Review primarily focuses on the structure-function relationship between the ligands and their reactivity and aims to provide suggestions for designing new and efficient catalysts for H generation from FA.
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http://dx.doi.org/10.1002/cssc.202100602DOI Listing
July 2021

Occurrence of per- and polyfluoroalkyl substances (PFASs) in wastewater of major cities across China in 2014 and 2016.

Chemosphere 2021 Sep 16;279:130590. Epub 2021 Apr 16.

Queensland Alliance for Environmental Health Sciences (QAEHS), The University of Queensland, Brisbane, QLD, 4102, Australia.

China produced and consumed a large amount of per- and polyfluoroalkyl substances (PFASs). whose persistency and possible toxicity to organisms have raised public health concerns. Analyzing influent wastewater could help to assess the composition and mass load of PFASs discharged into a wastewater treatment plant (WWTP) from its catchment. In this study, we analyzed 27 PFASs in wastewater samples collected from 42 WWTPs across China in 2014 and 2016. Results indicated that perfluorobutanoic acid (PFBA), perfluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) were the most common PFASs in wastewater. Population normalized mass loads of PFOA and PFOS were higher in Eastern China than in the other three regions, possibly due to their higher usage. Although the concentrations of PFASs in Central and West areas were showed lower than in East area, Wuhan (in Central area) and Lanzhou (in West area) were hotspots of PFASs pollution because of their industry structure. Population density and per capita Gross Domestic Product (GDP) have positive correlations with the concentration of PFBA, PFOA, PFHxA, and ∑PFASs in wastewater. The estimated annual release of PFASs to WWTPs in our study is much lower than the total emission to the environment. Our results suggest that although there was some reduction in the production volume, certain legacy PFASs were still released into wastewater and their composition and concentration vary among WWTPs.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130590DOI Listing
September 2021

Related norms of the ENGOs, one vital role in environmental improvement needed to be enhanced in China.

Environ Sci Pollut Res Int 2021 Jun 22;28(23):30366-30372. Epub 2021 Apr 22.

School of Liberal Arts, Nantong University, Nantong, 226019, China.

Four behavior subjects, including governments, enterprises, and public and environmental non-government organizations (abbreviated as ENGOs), all play vital roles in environmental protection. As an important component, the ENGOs of all the world focus on environmental improvement and provide services for the whole society. In developed countries, the ENGOs have been formed earlier, and all kinds of regulation, supervision, and maintenance systems on the organizations are relatively mature. The development of the ENGOs in China started late, but the development speed was very fast. Some issues, including the exploring on their existing situation and how to promote their role in environmental protection, should be concerned. In total, 2510 ENGOs' information in China was collected and analyzed in the study. The ENGOs in the nation were not uniformly distributed, and there were great differences in the number of the ENGOs among the 34 provinces of China. With a few exceptions, the ENGOs in China were generally small in the scale and poor in the viability. They were mostly research-oriented and often less participating. The organizations' development and improvement still needed a long-term arduous course. Many negative factors might influence the sustainable and healthy development of the ENGOs in China, and the legislation might be one cause. Several aspects-related norms, including the registration system, the financial support mechanism, and the identification on their plaintiff qualification, needed to be improved to encourage the development of these organizations and then promote the improvement of environmental quality better in China.
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http://dx.doi.org/10.1007/s11356-021-13981-9DOI Listing
June 2021

Kinetic Study on Continuous Sampling of Coal Char from a Micro Fluidized Bed.

ACS Omega 2021 Apr 24;6(13):9086-9094. Epub 2021 Mar 24.

State Key Laboratory of Coal Combustion, School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

We self-design a micro fluidized bed reactor (MFB) with combination of an online char particle sampling system to study the kinetics of coal char combustion and gasification. The system mainly contains two parts: a micro fluidized bed and vacuum online sampling. Vientiane coal was continuously sampled from the MFB. Both combustion and gasification reactivities of the sampled chars were tested in a thermogravimetric analyzer. Kinetic parameters of the sampled char were analyzed. Char reactivity in oxy-fuel combustion in the MFB obeys the rule of decrease-increase-decrease behavior with the sampling time. Pre-exponential factor and activation energy of the sampled char increase with the sampling time. The gasification reactivity of the sampled char increases with the sampling time even though there is a minor decrease in an initial gasification stage. The new designed MFB combining with the online sampling system will pave the path for the investigation of gas-solid reaction evolution in the future.
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http://dx.doi.org/10.1021/acsomega.1c00131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028157PMC
April 2021

A potential impact of SARS-CoV-2 on pituitary glands and pituitary neuroendocrine tumors.

Endocrine 2021 05 30;72(2):340-348. Epub 2021 Mar 30.

Department of Neurosurgery, Center of Pituitary Tumor, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China.

Introduction: Angiotensin-converting enzyme 2 (ACE2) is the receptor of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The effects of SARS-CoV-2 on normal pituitary glands function or pituitary neuroendocrine tumors (PitNETs) have not yet been elucidated. Thus, the present study aimed to investigate the potential risks of SARS-CoV-2 infection on the impairment of pituitary glands and the development of PitNETs.

Methods: PitNETs tissues were obtained from 114 patients, and normal pituitary gland tissues were obtained from the autopsy. The mRNA levels of ACE2 and angiotensin II receptor type 1 (AGTR1) were examined by quantitative real-time PCR. Immunohistochemical staining was performed for ACE2 in 69 PitNETs and 3 normal pituitary glands. The primary tumor cells and pituitary cell lines (MMQ, GH3 and AtT-20/D16v-F2) were treated with diminazene aceturate (DIZE), an ACE2 agonist, with various dose regimens. The pituitary hormones between 43 patients with SARS-CoV-2 infection were compared with 45 healthy controls.

Results: Pituitary glands and the majority of PitNET tissues showed low/negative ACE2 expression at both the mRNA and protein levels, while AGTR1 showed high expression in normal pituitary and corticotroph adenomas. ACE2 agonist increased the secretion of ACTH in AtT-20/D16v-F2 cells through downregulating AGTR1. The level of serum adrenocorticotropic hormone (ACTH) was significantly increased in COVID-19 patients compared to normal controls (p < 0.001), but was dramatically decreased in critical cases compared to non-critical patients (p = 0.003).

Conclusions: This study revealed a potential impact of SARS-CoV-2 infection on corticotroph cells and adenomas.
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http://dx.doi.org/10.1007/s12020-021-02697-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009460PMC
May 2021

The key roles of Fe-bearing minerals on arsenic capture and speciation transformation during high-As bituminous coal combustion: Experimental and theoretical investigations.

J Hazard Mater 2021 Aug 11;415:125610. Epub 2021 Mar 11.

State Key Laboratory of Coal Combustion, School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address:

The conversion of As vapor released from coal combustion to less hazardous solids is an important process to alleviate As pollution especially for high-As coal burning, but the roles of key ash components are still in debate. Here, we used multiple analytical methods across the micro to bulk scale and density functional theory to provide quantitative information on As speciation in fly ash and clarify the roles of ash components on As retention. Fly ash samples derived from the high-As bituminous coal-fired power plants showed a chemical composition of typical Class F fly ash. In-situ electron probe microanalysis (EPMA) was for the first time used to quantify and distinguish the inter-particle As distribution difference within coal fly ash. The spatial distribution of As was consistent with Fe, O, and sometimes with Ca. Grain-scale distribution of As in coal fly ash was quantified and As concentrations in single ash particles followed the order of Fe-oxides > aluminosilicates > unburned carbon > quartz. Sequential extraction and Wagner chemical plot of As confirmed that Fe minerals rather than Al-/Ca-bearing minerals played a vital role in capturing and oxidizing As into solid phase (As). Magnetite content in fly ash well-correlated with the increase ratio of As before and after magnetic separation, suggesting magnetite enhanced As enrichment in fly ash. Density functional theory (DFT) indicated that the bridges O sites of octahedral structure on FeO (111) surface were likely strong active sites for AsO adsorption. This study highlights the importance of magnetite on As transformation during bituminous or high-rank coal combustion in power plants and has great implications for developing effective techniques for As removal.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125610DOI Listing
August 2021

Multidimensional crosstalk between RNA-binding proteins and noncoding RNAs in cancer biology.

Semin Cancer Biol 2021 Mar 16. Epub 2021 Mar 16.

Center for Functional Genomics and Bioinformatics, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China. Electronic address:

RNA-binding proteins (RBPs) are well-known to bind RNA via a set of RNA-binding domains (RBDs) and determine the fate and function of their RNA targets; inversely, some RBPs, in certain cases, may be modulated by the bound RNAs rather than regulate their RNA partners. Current proteome-wide studies reveal that almost half of RBPs have no canonical RBDs, and the discovery of tens of thousands of noncoding RNAs (ncRNAs), especially those with the size larger than 200 nt (namely long noncoding RNAs, lncRNAs), makes the crosstalk between RBPs and RNAs more complicated. It is clear that macromolecular complexes formed by RBP and RNA are not only a form of existence of their RBP and RNA components in cells, but also represent a functional entity through which those RBPs and regulatory ncRNAs participate in the construction of regulatory networks in organism. In this review, we summarize the multidimensional crosstalk between RBPs and ncRNAs in cancer and discuss how RBPs achieve their function via the bound ncRNAs in different aspects of gene expression as well as how RBPs direct modification and processing of ncRNAs, in order to better understand tumor biology and provide new insights into development of strategies for cancer therapy and early detection.
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http://dx.doi.org/10.1016/j.semcancer.2021.03.007DOI Listing
March 2021

Discovery of novel N-benzylbenzamide derivatives as tubulin polymerization inhibitors with potent antitumor activities.

Eur J Med Chem 2021 Apr 24;216:113316. Epub 2021 Feb 24.

State Key Laboratory of Natural Medicines and Department of Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, PR China. Electronic address:

A series of novel N-benzylbenzamide derivatives were designed and synthesized as tubulin polymerization inhibitors. Among fifty-one target compounds, compound 20b exhibited significant antiproliferative activities with IC values ranging from 12 to 27 nM against several cancer cell lines, and possessed good plasma stability and satisfactory physicochemical properties. Mechanism studies demonstrated that 20b bound to the colchicine binding site and displayed potent anti-vascular activity. Notably, the corresponding disodium phosphate 20b-P exhibited an excellent safety profile with the LD value of 599.7 mg/kg (i.v. injection), meanwhile, it significantly inhibited tumor growth and decreased microvessel density in liver cancer cell H22 allograft mouse model without obvious toxicity. Collectively, 20b and 20b-P are novel promising anti-tubulin agents with more druggable properties and deserve to be further investigated for cancer therapy.
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http://dx.doi.org/10.1016/j.ejmech.2021.113316DOI Listing
April 2021

[Progress of studies on anti-breast tumor mechanism of alkaloids].

Zhongguo Zhong Yao Za Zhi 2021 Jan;46(2):312-319

Department of Pharmacy, Shanghai University of Medicine & Health Sciences Shanghai 201318, China Graduate School, Shanghai University of Chinese Medicine Shanghai 201203, China.

Breast tumor has become one of the malignant tumors with the highest incidence, and is a serious threat to human health, especially to women. Chemotherapy is an important anti-breast tumor therapy, which can be used in almost every stage of breast tumor therapy alone or in the combination with surgery and radiation therapy. Alkaloids are a kind of ubiquitous natural products, and important active components of various medicinal plants. A large number of studies have shown that alkaloids could exert an anti-breast tumor effect by inhibiting proliferation, metastasis and angiogenesis, resisting mitosis, promoting apoptosis and autophagy, and triggering cell cycle arrest. The extensive anti-breast tumor effect makes alkaloids an important candidate drug source. This paper reviews the anti-breast tumor mechanism of natural products of alkaloids.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20201015.601DOI Listing
January 2021

CD19 and CD30 CAR T-Cell Immunotherapy for High-Risk Classical Hodgkin's Lymphoma.

Front Oncol 2020 2;10:607362. Epub 2021 Feb 2.

Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University Guangdong, Guangzhou, China.

Background: In clinical applications of CAR T-cell therapy, life-threatening adverse events including cytokine release syndrome and neurotoxicity can lead to treatment failure. Outcomes of patients treated with anti-CD30 CAR T- cell have been disappointing in relapsing/refractory (r/r) classical Hodgkin's Lymphoma (cHL).

Methods: In order to understand the applicable population of multiple CAR T-cell therapy, we examined the expression of CD19, CD20, and CD30 by immunohistochemistry (IHC) in 38 paraffin-embedded specimens of cHL. In the past two years, we found only one patient with cHL who is eligible for combined anti-CD19 and CD30 CAR T-cell treatment. This patient's baseline characteristics were prone to severe adverse events. We treated this patient with low doses and multiple infusions of anti-CD19 and CD30 CAR T-cell.

Results: The positive expression of CD19 + CD30 in Reed-Sternberg (RS) cells is approximately 5.2% (2/38). The patient we treated with combined anti-CD19 and CD30 CAR T-cell did not experience severe adverse events related to CAR T-cell therapy and received long term progression-free survival (PFS).

Conclusion: For high risk r/r cHL patients, low doses of CAR T-cell used over different days at different times might be safe and effective. More clinical trials are warranted for CD19 and CD30 CAR T-cell combination therapy.
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http://dx.doi.org/10.3389/fonc.2020.607362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885818PMC
February 2021

Investigation and improvement of the desulfurization performance of molten carbonates under the influence of typical pyrolysis gases.

Waste Manag 2021 Apr 15;124:46-53. Epub 2021 Feb 15.

State Key Laboratory of Coal Combustion, School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

Co-pyrolysis with oxygen-lean waste tires could improve the quality of pyrolytic oil from the bio-wastes while HS/COS generated during co-pyrolysis process has a negative impact on the utilization of oil/syngas as well as the flue gas pollution control. Compared to traditional wet desulfurization process, high-temperature desulfurization via molten carbonates could reduce heat loss and favor the recycling of captured sulfur. Notably, small-molecule pyrolytic gases might change the species of sulfur-containing gases and promote the re-emission of absorbed sulfur from the molten salts. To fully understand the effects of pyrolysis gases (H/CO/HO/CO) on molten salts desulfurization efficiency as well as mutual conversion mechanism of HS and COS, equilibrium compositions calculations and adsorption experiments were carried out in the present study. The results showed that H/CO had few effects on molten salts desulfurization performance and mutual conversion of HS/COS. In contrast, CO and HO had obvious adverse effects on desulfurization efficiency through the transferring of free S into emitted sulfur-containing gases. More specifically, only a small amount of CO reacted with S to produce COS while more S was converted to HS and released from the reactor outlet when HO was introduced. Fortunately, the impact of HO or CO on molten salts desulfurization could be weakened with the addition of CaCO by transferring the molten free S into precipitated CaS. Besides, multi-stage desulfurization units connected in series and parallel were proposed and estimated, which was confirmed to show good performance to maintain the high desulfurization efficiency from the complicated pyrolytic gases.
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http://dx.doi.org/10.1016/j.wasman.2021.01.029DOI Listing
April 2021

Observations on the Particle Pollution of the Cities in China in the Coronavirus 2019 Closure: Characteristics and Lessons for Environmental Management.

Integr Environ Assess Manag 2021 Feb 10. Epub 2021 Feb 10.

School of Geographic and Oceanographic Science, Nanjing University, Nanjing, China.

Particulate matter in the air seriously affects human health and has been a hot topic of discussion. Because of the coronavirus disease 2019 (COVID-19) lockdown in cities in China, sources of particulate matter, including gasoline-burning vehicles, dust-producing building sites, and coal-fired factories, almost all ceased at the end of January 2020. It was not until early April that outdoor activities recovered. Ten cities were selected as observation sites during the period from 19 December 2019 to 30 April 2020, covering the periods of preclosure, closure, and gradual resumption. A total of 11 720 groups of data were obtained, and 4 indicators were used to assess the characteristics of the particle pollution in the period. The quality of the atmospheric environment was visibly influenced by human activities in those 5 mo. The concentrations of particulate matter with particle sizes below 10 µm (PM10) decreased slightly in February and March and then began to increase slowly after April with the gradual recovery of production. The concentrations of particulate matter with particle sizes below 2.5 µm (PM2.5) decreased greatly in most regions, especially in northern cities, during closure and maintained a relatively stable level in the following 3 mo. The trends of PM10 and PM2.5 indicated that the reduced human activities during the COVID-19 lockdown decreased the concentrations of particulate matter in the air, and the difference between the PM10 and PM2.5 trends might be due to the different sources of the 2 particles and their different aerodynamics. However, during closure, the particulate matter pollution in the cities remained at a high level, which indicated that some ignored factors other than outdoor production activities, automobile exhaust, and construction site dust might have contributed greatly to the PM10 and PM2.5 concentrations, and the tracing of the particulate matter should be given further attention in environmental management. Integr Environ Assess Manag 2021;00:1-11. © 2021 SETAC.
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http://dx.doi.org/10.1002/ieam.4399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014718PMC
February 2021
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