Publications by authors named "Hong Xu"

1,780 Publications

  • Page 1 of 1

Characterization of chitin-glucan complex from Tremella fuciformis fermentation residue and evaluation of its antibacterial performance.

Int J Biol Macromol 2021 Jun 9. Epub 2021 Jun 9.

College of Food and Light Industry, Nanjing Tech University, Nanjing 211800, PR China.

Submerged fermentation of fungi is an efficient way to obtain extracellular polysaccharides, however, in this process, excess discarded biomass is produced. In this study, Tremella fuciformis mycelia were reused as the raw material to isolate a novel fungal chitin-glucan complex (CGC-TFM) using alkaline extraction. Characteristic analysis revealed that the CGC-TFM consisted of glucosamine/acetylglucosamine and glucose (GlcN:Glc = 26:74 mol%), indicating a reference to the β polymorphism of chitin-glucan complex, with the molecular weight and crystallinity index of 256 ± 3.0 kDa and 54.25 ± 1.04%, respectively. Fourier transform infrared spectroscopy, X-ray diffraction, nuclear magnetic resonance, and scanning electron microscopy analyses confirmed that the chitin portion of the CGC-TFM exhibited a typical β configuration and N-acetylation degree of 70.52 ± 2.09%. Furthermore, the CGC-TFM exhibited good thermal stability and effective Escherichia coli inhibition ability, indicating that it could be applied as a potential food packaging material.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.06.048DOI Listing
June 2021

Infrastructure-driven development and sustainable development goals: Subjective analysis of residents' perception.

J Environ Manage 2021 Jun 8;294:112931. Epub 2021 Jun 8.

Department of Administration, Department of Administration, Zhou Enlai School of Government, Nankai University, China.

Belt and Road initiatives (BRI) transportation infrastructural development project started a new bilateral relationship amongst countries and acquired an important position to contribute to tourism and sustainable development goals (SDGs) 2030. The SDG1 (Poverty alleviation) and SDG 15 (Environmental protection) are globally highly challenging goals. Therefore, the current study using an integrative conceptual model addresses the fundamental role of BRI in achieving SDG 1 and SDG 15 in the presence of tourism development (TD) as a mediating variable. Based on stakeholder theory, using subjective analysis, the present study examines residents' perception of the BRI transportation infrastructural development, tourism development, SDG 1, and SDG 15. Using a self-administered questionnaire, collected a sample of 800 questionnaires data. The structural equation modelling analysis (SEM) indicated that the residents' perceive that the BRI transportation infrastructural development as a catalyst positively contributing to achieving SDG 1; however, it negatively correlates with SDG15. Besides, residents' perception of tourism development partially mediates between BRI transportation infrastructural development and SDG1 and SDG15. The study contributes to stakeholder theory and understanding residents' perceptions that may help key stakeholders in future policy and planning.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2021.112931DOI Listing
June 2021

Persistent Discrimination of TB in Southeastern China: Results from Four Repeated Population-Based Surveys During the Period of 2006-2018.

Risk Manag Healthc Policy 2021 3;14:2333-2344. Epub 2021 Jun 3.

Department of Tuberculosis Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.

Objective: To analyze the changes in discriminatory attitudes and isolated behaviors of the public toward tuberculosis (TB) in the Zhejiang Province and to determine the associated factors with TB discrimination.

Methods: Data were obtained from four cross-sectional population-based investigations from 2006 to 2018. A total of 26,246 respondents were interviewed using unified questionnaires that measured knowledge, attitudes, and behaviors regarding TB. The changes in public attitudes and behaviors towards TB over time were analyzed. The effect of socio-demographic factors and the level of TB awareness on TB discriminatory attitudes and isolated behaviors were evaluated.

Results: The results of these four cross-sectional studies found that TB discrimination had not changed much over the decade. Overall, discriminatory attitudes were present in 63.5% of the respondents who knew about TB (81.2%). Nearly 31.2% of those who reported being surrounded by people with TB (5.8%) showed isolated behaviors. Older respondents, those with a low education level, and farmers were prone to having discriminatory attitudes or behaviors. Those aware of the infectiousness and transmission routes of TB, and those who felt that TB was serious were more likely to discriminate against TB ( < 0.001). Those aware that TB can be cured were non-discriminatory (aOR = 0.77, 95% CI: 0.72-0.82).

Conclusion: Discriminatory attitudes and isolated behaviors toward TB have not changed significantly in southeastern China over the survey years, and persistent discrimination against TB still exists among the public. The multiple causes of discrimination cannot be addressed through basic health education. Tailor-made strategies, relevant policy measures, and an enabling social environment for TB are urgently required.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/RMHP.S311869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184293PMC
June 2021

Comparative study on the pharmacodynamic difference of oral administration of Xiaojin Pills accompanied with Chinese Baijiu and water.

J Ethnopharmacol 2021 Jun 7:114284. Epub 2021 Jun 7.

State Key Laboratory of Southwestern Chinese Medicine Resources, Pharmacy School, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China. Electronic address:

Ethnopharmacological Relevance: Xiaojin Pills is a classic prescription for the treatment of mammary glands hyperplasia with a history of nearly 300 years, and is also the first choice of Chinese patent medicine for the clinical treatment of mammary glands hyperplasia in contemporary traditional Chinese medicine clinic. Clinical and animal studies have shown that Xiaojin Pills has the effects of anti-mammary glands hyperplasia, promoting blood circulation, anti-inflammation and analgesia. However, its initial administration method was "taking orally after soaked with Chinese Baijiu", the modern method was changed to "taking orally with water" in recent 20 years. Whether there is any difference in the efficacy of the two administration methods is still unknown.

Aim Of The Study: To reveal the difference in efficacy and metabolic mechanism of anti-mammary gland hyperplasia between the oral administration of Xiaojin Pills accompanied with Chinese Baijiu (XJP&B) and water (XJP&W).

Materials And Methods: COX-2 inhibition rate test and anti-platelet aggregation activity test were used to investigate the efficacy difference between the 40 Vol% Chinese Baijiu and water extracts of Xiaojin Pills on anti-inflammatory and blood-activating in vitro. Kunming male mice (20 ± 5 g) and SD female rats (200-220 g) were orally treated with XJP&B and XJP&W, respectively. Then the difference in anti-inflammatory and analgesic effects between XJP&B and XJP&W were evaluated via xylene-induced ear swelling test, formaldehyde-induced pain test, and acetic acid-induced writhing test in mice. Determination of nipple diameter, pathological examination of mammary gland tissue, determination of serum E, P and FSH content and hemorheological parameters of rats with mammary gland hyperplasia were performed to explore the efficacy difference in anti-mammary gland hyperplasia between XJP&B and XJP&W. Metabolomics was used to study the difference of anti-mammary gland hyperplasia mechanism between XJP&B and XJP&W.

Results: The results showed that the effect of XJP&B was superior to that of XJP&W in anti-platelet aggregation, inhibition of inflammation and pain, and anti-mammary gland hyperplasia. Interestingly, the advantages were more significant under low-dose condition. In addition, the mechanism of the two combinations against mammary gland hyperplasia was indeed different. Their common metabolic pathways include tryptophan metabolism and alanine, aspartic acid and glutamic acid metabolism. However, Chinese Baijiu and XJP&B also have additional regulatory effects on linoleic acid metabolic pathway.

Conclusion: In brief, this research demonstrated that the efficacy of XJP&B was better than that of XJP&W in activating the blood, anti-inflammation, analgesia and anti-mammary gland hyperplasia, which means that XJP&B has synergistic and superior effects. The special dose-effect relationship under the condition of XJP&B was also found, laying the foundation for clinical treatment to reduce the dosage and shorten the medication cycle, which is beneficial to reduce the economic burden of patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2021.114284DOI Listing
June 2021

Hydroxide Anion Transport in Covalent Organic Frameworks.

J Am Chem Soc 2021 Jun 10. Epub 2021 Jun 10.

Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

Hydroxide anion transport is essential for alkaline fuel cells, but hydroxide anion has an inherently low conductivity owing to its small diffusion coefficient and high mass. Ordered open channels found in covalent organic frameworks are promising as pathways to enable hydroxide anion transport, but this remains to be explored. Here we report designed synthesis of anionic covalent organic frameworks that promote hydroxide anion transport across the one-dimensional channels. Engineering cationic chains with imidazolium termini onto the pore walls self-assembles a supramolecular interface of single-file hydroxide anion chains in the channels. The frameworks facilitate hydroxide anion transport to achieve an exceptional conductivity of 1.53 × 10 S cm at 80 °C, which is 2-6 orders of magnitude higher than those of linear polymers and other porous frameworks. Impedance spectroscopy at different temperatures and studies on deuterated samples reveal that hydroxide anions transport via a proton-exchange hopping mechanism. These results open a way to design framework materials for energy conversions via engineering an anionic interface.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.1c03268DOI Listing
June 2021

Dynamic Coordination between a Triphenylamine-Functionalized Salicylaldehyde Schiff Base and a Copper(II) Ion.

Inorg Chem 2021 Jun 7. Epub 2021 Jun 7.

Guangdong Provincial Key Laboratory of Functional Soft Condensed Matter, School of Material and Energy, Guangdong University of Technology, Guangzhou 510006, P. R. China.

The coordination between a ligand and a metal is a spontaneous and uncontrollable process. In this Article, we successfully observe the formation of metal coordination in a triphenylamine-functionalized salicylaldehyde Schiff base with a copper(II) ion. The ligand first reacts with Cu in a stepwise process to afford the dynamic complex ([ligand]:[Cu] = 1:1), which further reacts with an extra copper(II) ion to afford with the following stepwise (or cumulative) stability constants: = 4.0694 × 10 and = 1.0761 × 10, respectively. The entire metal coordination process can be visualized, and the coordination mode of the probe toward copper was further evaluated by ultraviolet-visible/fluorescence spectra, single-crystal X-ray diffraction, density functional theory calculations, high-resolution mass spectra, and nuclear magnetic resonance spectroscopic titrations. Compound exhibited excellent sensitivity and specificity toward copper(II) ions in THF/water media with a low limit of detection of 2.687 × 10 mol L. In addition, can be applied to the detection of Cu in real samples with satisfactory recoveries in the range of 100-112% in lake water and 98-101% in tap water. This Article not only reports an excellent fluorescence probe for copper(II) ion detection but also presents an instance for more fully understanding the metal coordination process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.inorgchem.1c00523DOI Listing
June 2021

Combined exome sequencing and deep phenotyping in highly selected fetuses with skeletal dysplasia during the first and second trimesters improves diagnostic yield.

Prenat Diagn 2021 Jun 6. Epub 2021 Jun 6.

Department of Obstetrics and Gynecology, 1st Medical Center of Chinese PLA General Hospital, Beijing, China.

Objective: To investigate the genetic etiology of skeletal dysplasia in highly selected fetuses during the first and second trimesters using deep phenotyping and exome sequencing (ES).

Method: Fetuses with short femurs were identified using the established prenatal diagnostic approach. A multidisciplinary team reviewed fetal phenotypic information (prenatal ultrasound findings, fetal postmortem, and radiographs) in a cohort of highly selected fetuses with skeletal dysplasia during the first and second trimesters. The affected families underwent multiplatform genetic tests.

Results: Of the 27 affected fetuses, 21 (77.8%) had pathogenic or potential pathogenic variations in the following genes: COL1A1, FGFR3, COL2A1, COL1A2, FLNB, DYNC2LI1, and TRIP11. Two fetuses had compound heterozygous mutations in DYNC2LI1 and TRIP11, respectively, and the other 19 carried de novo autosomal dominant variants. Novel variants were identified in COL1A1, COL2A1, COL1A2, DYNC2LI1, and TRIP11 in 11 fetuses. We also included the first description of the phenotype of odontochondrodysplasia in a prenatal setting.

Conclusions: ES or panel sequencing offers a high diagnostic yield for fetal skeletal dysplasia during the first and second trimesters. Comprehensive and complete phenotypic information is indispensable for genetic analysis and the expansion of genotype-phenotype correlations in fetal skeletal abnormalities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/pd.5974DOI Listing
June 2021

Mucosal microbial microenvironment in early gastric neoplasia and non-neoplastic gastric disease.

J Gastroenterol Hepatol 2021 Jun 5. Epub 2021 Jun 5.

Department of Gastroenterology and Endoscopy Center, The First Hospital of Jilin University, Changchun, China.

Background And Aim: The biological characterization of microbial environment in early gastric cancer (EGC), other than Helicobacter pylori, is limited. This study aimed to explore the microbial microenvironment in chronic gastritis (CG), fundic gland polyps (FGPs), low-grade intraepithelial neoplasia (LGIN) and EGC.

Methods: 16S-rRNA gene sequencing and bioinformatic analysis were performed on 63 individuals with 252 mucosal biopsies or endoscopic submucosal dissection margin samples from endoscopy.

Results: The microbiota in gastric LGIN functions analogously to EGC in terms of functional prediction. Neoplastic lesions showed a significant difference to CG or FGPs in beta diversity of the microbiota. Bacteria genera including Paracoccus, Blautia, Barnesiella, Lactobacillus, Thauera, Collinsella were significantly enriched in gastric neoplastic mucosa (LGIN and EGC) compared to non-neoplastic tissues (CG and FGPs). Whilst Pseudomonas and Kingella were depleted in neoplastic tissues. FGPs showed a distinctive microbial network system that negatively interacted with Helicobacter.

Conclusions: In terms of the mucosal microbial microenvironment, gastric LGIN and EGC showed no significant difference as early neoplastic lesions. We observed a coordinated microbial microenvironment that correlated negatively with Helicobacter.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jgh.15565DOI Listing
June 2021

Influence of Texture on the Mechanical Properties of a Mg-6Al-1Zn-0.9Sn Alloy Processed by ECAP.

Materials (Basel) 2021 May 19;14(10). Epub 2021 May 19.

Key Laboratory of Automobile Materials of Ministry of Education, School of Materials Science and Engineering, Nanling Campus, Jilin University, No. 5988 Renmin Street, Changchun 130025, China.

The microstructure and mechanical properties of a Mg-6Al-1Zn-0.9Sn alloy processed by equal channel angular pressing (ECAP) at temperatures of 250 °C and 300 °C were investigated. It was found that the refinement of the microstructure was very dependent on the processing temperature. The main reason for the difference in grain refinement was the precipitation of secondary-phase particles. Texture information obtained by electron back-scatter diffraction (EBSD) showed the gradual formation of a 45° texture during the ECAP process, while the maximum intensity was different for processing temperatures at 250 °C and 300 °C. By calculating the contribution from different strengthening mechanisms, it was found that a 45° texture had a huge influence on grain boundary strengthening and thus the yield strength.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14102664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160751PMC
May 2021

A 1-Hydroxy-2,4-Diformylnaphthalene-Based Fluorescent Probe and Its Detection of Sulfites/Bisulfite.

Molecules 2021 May 21;26(11). Epub 2021 May 21.

The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550004, China.

A novel 1-hydroxy-2,4-diformylnaphthalene-based fluorescent probe was synthesized by a Knoevenagel reaction and exhibited excellent sensitivity and selectivity towards sulfite ions (SO) and bisulfite ions (HSO). The detection limits of the probe were 0.24 μM using UV-Vis spectroscopy and 9.93 nM using fluorescence spectroscopy, respectively. Furthermore, the fluorescent probe could be utilized for detection in real water samples with satisfactory recoveries in the range 99.20%~104.30% in lake water and 100.00%~104.80% in tap water by UV-Vis absorption spectrometry, and in the range 100.50%~108.60% in lake water and 102.70%~103.80% in tap water by fluorescence spectrophotometry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules26113064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196617PMC
May 2021

Responsible genes in children with primary vesicoureteral reflux: findings from the Chinese Children Genetic Kidney Disease Database.

World J Pediatr 2021 May 31. Epub 2021 May 31.

Department of Nephrology, Children's Hospital of Fudan University, National Children's Medical Center, 399 Wan Yuan Road, Shanghai, 201102, People's Republic of China.

Background: Primary vesicoureteral reflux (VUR) is a common congenital anomaly of the kidney and urinary tract (CAKUT) in childhood. The present study identified the possible genetic contributions to primary VUR in children.

Methods: Patients with primary VUR were enrolled and analysed based on a national multi-center registration network (Chinese Children Genetic Kidney Disease Database, CCGKDD) that covered 23 different provinces/regions in China from 2014 to 2019. Genetic causes were sought using whole-exome sequencing (WES) or targeted-exome sequencing.

Results: A total of 379 unrelated patients (male: female 219:160) with primary VUR were recruited. Sixty-four (16.9%) children had extrarenal manifestations, and 165 (43.5%) patients showed the coexistence of other CAKUT phenotypes. Eighty-eight patient (23.2%) exhibited impaired renal function at their last visit, and 18 of them (20.5%) developed ESRD at the median age of 7.0 (IQR 0.9-11.4) years. A monogenic cause was identified in 28 patients (7.39%). These genes included PAX2 (n = 4), TNXB (n = 3), GATA3 (n = 3), SLIT2 (n = 3), ROBO2 (n = 2), TBX18 (n = 2), and the other 11 genes (one gene for each patient). There was a significant difference in the rate of gene mutations between patients with or without extrarenal complications (14.1% vs. 6%, P = 0.035). The frequency of genetic abnormality was not statistically significant based on the coexistence of another CAKUT (9.6% vs. 5.6%, P = 0.139, Chi-square test) and the grade of reflux (9.4% vs. 6.7%, P = 0.429). Kaplan-Meier survival curve showed that the presence of genetic mutations did affect renal survival (Log-rank test, P = 0.01). PAX2 mutation carriers (HR 5.1, 95% CI 1.3-20.0; P = 0.02) and TNXB mutation carriers (HR 20.3, 95% CI 2.4-168.7; P = 0.01) were associated with increased risk of progression to ESRD.

Conclusions: PAX2, TNXB, GATA3 and SLIT2 were the main underlying monogenic causes and accounted for up to 46.4% of monogenic VUR. Extrarenal complications and renal function were significantly related to the findings of genetic factors in children with primary VUR. Like other types of CAKUT, several genes may be responsible for isolated VUR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12519-021-00428-xDOI Listing
May 2021

Basophils as a potential marker of lupus nephritis by flow cytometry.

Future Sci OA 2021 Feb 16;7(5):FSO690. Epub 2021 Feb 16.

Department of Rheumatology & Immunology, West China Hospital, Sichuan University, Chengdu, China.

Objective: To establish a convenient and simple flow cytometry immunophenotyping panel to explore immune cellular alterations and potential cellular biomarkers in systemic lupus erythematosus.

Materials And Methods: This is a cross-sectional, case-control study including 60 patients with systemic lupus erythematosus and 20 sex- and age-matched healthy controls. A 14-color immunophenotyping panel was applied to detect proportions of circulating immune mononuclear cells, and comparisons between patients and healthy controls, and subgroups of patients, were performed. Correlations between cellular proportions and other parameters were investigated.

Results: After multivariate analysis, significantly decreased proportions of CD4CD8 T cells, natural killer cells and innate lymphoid cells were observed in patients compared with healthy controls. The proportions of basophils were decreased significantly in patients with lupus nephritis (LN) compared with those in patients without LN.

Conclusion: In the present study, we found that basophil proportions may be a biomarker of LN.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2144/fsoa-2020-0212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147755PMC
February 2021

T cell engaging bispecific antibodies targeting CD33 IgV and IgC domains for the treatment of acute myeloid leukemia.

J Immunother Cancer 2021 May;9(5)

Pediatrics, Memorial Sloan Kettering Cancer Center, New York, New York, USA

Background: Acute myeloid leukemia (AML) remains one of the most challenging hematological malignancies. Despite progress in therapeutics, majority of patients succumb to this neoplasm. CD33 is a proven therapeutic target, given its expression on most AML cells. Almost all anti-CD33 antibodies target the membrane distal immunoglobulin V (IgV) domain of the CD33 extracellular domain.

Methods: In this manuscript, we present data on three bispecific antibodies (BsAbs) against the CD33 IgV and membrane proximal immunoglobulin C (IgC) domains. We use in vitro binding and cytotoxicity assays to show the effect of these BsAbs on AML cell lines. We also use immunodeficient mice-bearing leukemias from cell lines and patient-derived xenografts to show the effect of these BsAbs in vivo.

Results: In vitro, the IgV-targeting BsAb had higher binding to AML cell lines using flow cytometry and delivered more potent cytotoxicity in T-cell-dependent cytotoxicity assays; importantly, the IgC domain-targeting outperformed the IgV domain-targeting BsAb in medullary and extramedullary leukemia animal models.

Conclusions: These data support further clinical development of this BsAb for first-in-human phase I clinical trial.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/jitc-2021-002509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154967PMC
May 2021

[Formation and Prevention of Secondary Nitrate in PM in Tianjin].

Huan Jing Ke Xue 2021 Jun;42(6):2616-2625

Tianjin Eco-Environmental Monitoring Center, Tianjin 300191, China.

To study the formation and approaches to controlling secondary nitrate in PM, the ionic compositions of PM, pH of aerosols, variations in NH-NH and HNO-NO concentrations, and the joint NH/HNO sensitivity regime map of ammonium nitrate were investigated based on high-resolution online monitoring data for an urban site in central Tianjin from 2018 to 2019. The results showed that the average concentration of PM was 58μg·m, and the main ionic compositions of PM were nitrate (NO), ammonium (NH), sulfate (SO), Cl, and K with corresponding mass percentages of 18.4%, 11.6%, 10.3%, 3.3%, and 2.6%, respectively. Concentrations of PM and the main components were relatively high during the heating season and relatively low during the non-heating season. The aerosols were weakly acidity with an average pH of 5.21; pH was higher in spring and winter and lower in summer and autumn, and diurnal variations pH were lower in the morning (00:00-08:00) and slightly higher at other times. The concentrations of NH(g) (gas NH) and HNO(g) (gas HNO) were 16.7μg·mand 1.2μg·m, respectively. The concentrations of NH(g) were relatively higher from April to September and lower from October to February of the following year. HNO(g) concentrations did not show any clear monthly pattern. Except during the summer, NH(g) concentrations were higher in the morning and evening, and HNO(g) concentrations were higher during the day. No clear linear relationships were observed between the concentrations of NH(g) and NH nor the concentrations of HNO(g) and NO at different pH levels. Higher concentrations of NO and NH were observed in the morning and evening, while no linear relationships were observed between the pH and concentrations of NH(g)-NH and HNO(g)-NO. The joint NH/HNO sensitivity regime map showed that most of the points were located in the HNO sensitive region with some in the NH & HNO sensitive region. In spring, autumn, and winter, most of the points were located in the HNO sensitive region while in summer, a significant quantity of the points were located in the NH & HNO sensitive region. Therefore, the precursors of HNO (such as NO) should be controlled in the spring, autumn, and winter, and attention should be given to the control of the precursors of HNO (NO) and NH in the summer to effectively control nitrate and ammonium aerosols in PM in Tianjin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13227/j.hjkx.202010119DOI Listing
June 2021

Diagnostic and clinical utility of genetic testing in children with kidney failure.

Pediatr Nephrol 2021 May 24. Epub 2021 May 24.

Department of Nephrology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, 201102, China.

Background: Genetic kidney disease is well established as an important cause of pediatric kidney failure, and genetic testing might increase diagnostic accuracy, but evidence is limited. This study was conducted to determine the diagnostic yield and clinical impact of genetic testing for children with kidney failure.

Methods: Patients who were diagnosed with kidney failure before 19 years of age at Children's Hospital of Fudan University from 2009 to 2018 and received next-generation sequencing (NGS) were enrolled. The results for likely pathogenic variants in genes known to cause chronic kidney disease (CKD) were analyzed.

Results: A molecular diagnosis was identified in 39.9% (75/188) of children with kidney failure. Specific subtype of clinical category was discerned in 54 (72.0%) patients, kidney disease was reclassified in 7 (9.3%) patients, the unknown etiology of 5 (6.7%) patients was molecularly diagnosed, and the clinical diagnoses of the other 9 (12.0%) patients were confirmed. In addition, genetic diagnosis was considered to have contributed to clinical management, including negating the need for kidney biopsy (26/75, 34.7%), avoiding immunosuppressive therapy (24/75, 32.0%), changing surveillance (48/75, 64.0%), guiding specific treatment (21/75, 28.0%), and guiding peri-transplant management and options for kidney transplantation (12/75, 16.0%). Furthermore, cascade testing was subsequently offered to 34.7% (26/75) of families.

Conclusions: Genetic testing identified a molecular diagnosis in nearly 40% of children with kidney failure. Our results confirm that in children with kidney failure, genetic testing can not only establish a specific molecular diagnosis, but has a significant impact on clinical management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00467-021-05141-5DOI Listing
May 2021

Phase, microstructure and service character of as-deposited and short-time heat-treated Ni-Mo alloys with mixed state.

PLoS One 2021 21;16(5):e0249875. Epub 2021 May 21.

College of Engineering of Zhejiang Normal University, Jinhua, China.

Considering the amorphous and nano-crystalline cluster structure and their activity, on the basis of the mixed structure Ni-Mo alloys, the crystallization kinetics of the alloys and the performance of the alloys after heat treatment with different mixed structure were studied. The phase structure and composition were determined by X-ray powder diffraction. The crystallization activation energy of the mixed structure was obtained by differential scanning calorimetry. The electrochemical activity of the mixed structure alloy was determined by electrochemical analysis. The experimental results show that the structural stability of the mixed-structure alloy is better, but the crystallization activation energy is much lower than that of the amorphous alloy. The crystallization process consists of a meta-stable structure transition and a new phase formation. The electrochemical properties of the alloy indicated that the alloy with the mixed structure has higher electrochemical activity, with higher hardness and better corrosion resistance, which results from the large true contact surface and the large number of active centers in this material structure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249875PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139472PMC
May 2021

Effect of predictive trauma care on fracture healing and complications of traumatic fracture patients in emergency department.

Am J Transl Res 2021 15;13(4):3752-3758. Epub 2021 Apr 15.

Department of Oncology, Affiliated Hospital of Jiangnan University Wuxi, Jiangsu Province, China.

Objective: To explore the advantages of predictive trauma care in fracture healing and prevention of complications in patients with traumatic fracture.

Methods: Through prospective research methods, 80 patients with traumatic fractures were divided into a research group and a control group, each with 40 cases. The control group was given regular emergency care, while the research group was given predictive trauma care. The related clinical indicators, complications and scores on changes in joint range of motion (ROM), mental status and quality of life before and after intervention were compared between the two groups.

Results: Compared with the control group, the time of pain relief, fracture recovery and hospital stay of the research group was significantly shortened (all P<0.01). The overall incidence of complications in the research group was lower than that in the control group (5.00% vs 20.00%, P<0.05). For patients with limb fractures, the ROM scores of the two groups after intervention were significantly higher than those before intervention, and the scores of the research group were higher than those of the control group (all P<0.05). Compared with the condition before intervention, the Hamilton Anxiety Scale (HAMA) and Hamilton Depression Scale (HAMD) scores of the two groups of patients were significantly reduced after intervention, and the scores of research group were lower than those of the control group (all P<0.05). Compared with the condition before intervention, the scores of physical function, social function, psychological function and daily life condition of the two groups of patients were significantly increased 3 months after discharge from hospital, and the scores of the research group were significantly higher than those of the control group (all P<0.05).

Conclusion: Predictive trauma care can effectively reduce the risk of postoperative complications in patients with traumatic fractures, promote the fracture healing, relieve the patients' anxiety and depression, and improve their quality of life.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129251PMC
April 2021

Effect of PDCA-based nursing management model on the quality of life and complications of patients with acute leukemia undergoing chemotherapy.

Am J Transl Res 2021 15;13(4):3246-3253. Epub 2021 Apr 15.

Department of Emergency, Affiliated Hospital of Jiangnan University Wuxi, Jiangsu Province, China.

Objective: To assess the influence of PDCA-based nursing management model on the quality of life (QOL) and complications of patients with acute leukemia undergoing chemotherapy.

Methods: In this study, we randomly divided 118 patients with acute leukemia undergoing chemotherapy in our hospital into an observation group (n=59) and a control group (n=59). The control group was implemented routine nursing while the observation group was implemented PDCA-based nursing management. The anxiety, depression, QOL, cancer fatigue, total complication rate, sleep quality, and patients' satisfaction were compared between the two groups.

Results: The SAS and SDS scores of the observation group were significantly lower than those of the control group (P<0.05). The scores of physical function, general health, social function, emotional role, and mental health of the observation group were superior to those of the control group (all P<0.05). The 4 dimensions of behavior/severity, sense, emotion and cognition/mood in the observation group were significantly lower than those of the control group (all P<0.05). The incidence of complications such as nausea and vomiting, infection, bleeding, phlebitis, bone marrow suppression and mucositis in the observation group was significantly lower than that in the control group (P<0.05). The scores of sleep quality in the observation group were significantly lower than those of the control group (all P<0.05). The observation group's satisfaction with PDCA-based nursing management was markedly higher than that of the control group (P<0.05).

Conclusion: The PDCA-based nursing management model applied to patients with acute leukemia undergoing chemotherapy has shown good effects, which can improve depression, anxiety, cancer fatigue, sleep quality and QOL. It also significantly reduced the incidence of complications and improved patients' satisfaction, which was worth of clinical application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129232PMC
April 2021

Importance of clinical practice guidelines to practicing pediatric nephrologists and IPNA survey.

Pediatr Nephrol 2021 May 20. Epub 2021 May 20.

Department of Pediatric Kidney, Liver and Metabolic Diseases, Children's Hospital, Hannover Medical School, Carl-Neuberg-Str 1, 30625, Hannover, Germany.

Clinical practice guidelines (CPGs) are systematically developed statements backed by scientific evidence to assist practitioners in management in clinical practice. An international cross-sectional survey was conducted by the IPNA to examine the perceptions of pediatric nephrologists on guidelines and their usage and to identify important diseases for future clinical practice guidelines (CPGs). The survey found that the majority of pediatric nephrologists find CPGs useful in clinical practice and admitted to using them most of the time. Developing CPGs is challenging and there are standards available to develop trustworthy guidelines. While evidence-based global guidelines are ideal, pediatric nephrologists expressed the desire that they address regional differences. Most respondents (89.2%) to the survey agreed that adult guidelines did not cover the pediatric perspective adequately and 71.4% opined that consensus-based pediatric guidelines can be developed when evidence for the pediatric population is lacking. The development of high-quality practice guidelines requires substantial resources and may not be feasible in resource-poor countries. Adaptation of an existing guideline has been suggested as an alternative and the ADAPTE collaboration provides a systematic approach to adapting guidelines. Several diseases where pediatric guidelines are needed as a priority including IgA and C3 glomerulopathy were identified in the survey. Implementation of guideline-based care is challenging and the survey found that lack of availability of guidelines (43%) and resources (22.8%) are important reasons for poor implementation in lower-middle and low-income countries. Perceived complexity of guidelines, physician attitudes, and lack of training also contribute to non-adherence to guidelines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00467-021-05105-9DOI Listing
May 2021

Recommendations regarding the admission, infection prevention and control of pediatric patients during coronavirus disease 2019 outbreak in Shanghai China.

Transl Pediatr 2021 Apr;10(4):692-700

Department of Nephrology/Rheumatology, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/tp-20-271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107857PMC
April 2021

One-Pass Conversion of Benzene and Syngas to Alkylbenzenes by Cu-ZnO-AlO and ZSM-5 Relay.

Catal Letters 2021 May 13:1-13. Epub 2021 May 13.

Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, 030001 Shanxi Province China.

Alkylbenzenes have a wide range of uses and are the most demanded aromatic chemicals. The finite petroleum resources compels the development of production of alkylbenzenes by non-petroleum routes. One-pass selective conversion of benzene and syngas to alkylbenzenes is a promising alternative coal chemical engineering route, yet it still faces challenge to industrialized applications owing to low conversion of benzene and syngas. Here we presented a Cu-ZnO-AlO/ZSM-5 bifunctional catalyst which realizes one-pass conversion of benzene and syngas to alkylbenzenes with high efficiency. This bifunctional catalyst exhibited high benzene conversion (benzene conversion of 50.7%), CO conversion (CO conversion of 55.0%) and C7&C8 aromatics total yield (C7&C8 total yield of 45.0%). Characterizations and catalytic performance evaluations revealed that ZSM-5 with well-regulated acidity, as a vital part of this Cu-ZnO-AlO/ZSM-5 bifunctional catalyst, substantially contributed to its performance for the alkylbenzenes one-pass synthesis from benzene and syngas due to depress methanol-to-olefins (MTO) reaction. Furthermore, matching of the mass ratio of two active components in the dual-function catalyst and the temperature of methanol synthesis with benzene alkylation reactions can effectively depress the formation of unwanted by-products and guarantee the high performance of tandem reactions.

Supplementary Information: The online version contains supplementary material available at 10.1007/s10562-021-03617-5.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10562-021-03617-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116197PMC
May 2021

Integrating Population Variants and Protein Structural Analysis to Improve Clinical Genetic Diagnosis and Treatment in Nephrogenic Diabetes Insipidus.

Front Pediatr 2021 29;9:566524. Epub 2021 Apr 29.

Department of Nephrology, National Pediatric Medical Center of China, Children's Hospital of Fudan University, Shanghai, China.

Congenital nephrogenic diabetes insipidus (NDI) is a rare genetic disorder characterized by renal inability to concentrate urine. We utilized a multicenter strategy to investigate the genotype and phenotype in a cohort of Chinese children clinically diagnosed with NDI from 2014 to 2019. Ten boys from nine families were identified with mutations in or along with dehydration, polyuria-polydipsia, and severe hypernatremia. Genetic screening confirmed the diagnosis of seven additional relatives with partial or subclinical NDI. Protein structural analysis revealed a notable clustering of diagnostic mutations in the transmembrane region of and an enrichment of diagnostic mutations in the C-terminal region of . The pathogenic variants are significantly more likely to be located inside the domain compared with population variants. Through the structural analysis and prediction, the eight mutations identified in this study were presumed to be disease-causing. The most common treatments were thiazide diuretics and non-steroidal anti-inflammatory drugs (NSAIDs). Emergency treatment for hypernatremia dehydration in neonates should not use isotonic saline as a rehydration fluid. Genetic analysis presumably confirmed the diagnosis of NDI in each patient in our study. We outlined methods for the early identification of NDI through phenotype and genotype, and outlined optimized treatment strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fped.2021.566524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116627PMC
April 2021

Population Pharmacokinetics and Initial Dose Optimization of Sirolimus Improving Drug Blood Level for Seizure Control in Pediatric Patients With Tuberous Sclerosis Complex.

Front Pharmacol 2021 20;12:647232. Epub 2021 Apr 20.

Department of Pharmacy, Children's Hospital of Fudan University, Shanghai, China.

The purposes of this study were to explore the population pharmacokinetics and initial dose optimization of sirolimus improving drug blood level for seizure control in pediatric patients with tuberous sclerosis complex (TSC). Eighty pediatric patients diagnosed with TSC-related epilepsy were included for analysis. Sirolimus concentrations, physiological and biochemical indexes, and drug combination were collected to build a nonlinear mixed effect (NONMEM) model. Initial dose optimization was simulated by the Monte Carlo method. The weight and concomitant medication of oxcarbazepine affected sirolimus clearance. Without oxcarbazepine, for once-daily sirolimus regimen, the doses of 0.07, 0.06, 0.05, 0.04, and 0.03 mg/kg/day were recommended for weights of 5-7.5, 7.5-11.5, 11.5-19, 19-40, and 40-70 kg, respectively; for twice-daily sirolimus regimen, the doses of 0.05, 0.04, and 0.03 were recommended for weights of 5-8, 8-20, and 20-70 kg, respectively. With oxcarbazepine, for once-daily sirolimus regimen, the doses of 0.09, 0.08, 0.07, 0.06, 0.05, and 0.04 mg/kg/day were recommended for weights of 5-7.5, 7.5-10, 10-13.5, 13.5-20, 20-35, and 35-70 kg, respectively; for twice-daily sirolimus regimen, the doses of 0.06, 0.05, 0.04, and 0.03 were recommended for weights of 5-7, 7-14.5, 14.5-38, and 38-70 kg, respectively. The present study was the first to establish a population pharmacokinetic model of sirolimus improving drug blood level for seizure control in pediatric patients with TSC and recommend the initial dosage regimen.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2021.647232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114543PMC
April 2021

Prognostic value of circulating tumor DNA in lymphoma: a meta-analysis.

Clin Exp Med 2021 May 15. Epub 2021 May 15.

Department of Hematology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China.

Circulating tumor DNA (ctDNA) can be used to evaluate the prognosis of lymphoma. However, there is no uniform consensus about the mechanistic role that ctDNA plays in the prognosis of lymphoma. This meta-analysis explores the prognostic value of ctDNA in lymphoma, especially in diffuse large B cell lymphoma (DLBCL). All relevant reports published as of May 14, 2020, were retrieved by searching electronic databases in Pubmed, Embase and Cochrane Library. The prognostic value of ctDNA was evaluated using meta-analysis. Revman 5.3 software was used for prognostic data extraction and analysis. Eight studies, including a total of 767 lymphoma patients, were enrolled in this meta-analysis. Five out of eight studies investigated the association between ctDNA levels and progression-free survival (PFS) in 501 lymphoma patients, indicating that high levels of ctDNA were significantly associated with poor PFS (HR 2.24, 95%CI: 1.63-3.08, P < 0.00001). We conducted a subgroup analysis of 379 patients with DLBCL across three of the studies and came to the same conclusion (HR 2.01, 95%CI: 1.42-2.85, P < 0.0001). Two studies with a total of 192 lymphoma patients described the association between ctDNA levels and event-free survival (EFS), showing that high levels of ctDNA were also associated with adverse EFS (HR 4.53, 95%CI: 1.79-11.47, P = 0.001). The remaining two studies analyzed the potential clinical value of ctDNA for predicting the overall survival time (OS) of DLBCL patients, demonstrating that high levels of ctDNA correlated with inferior OS (HR 3.09, 95%CI: 1.50-6.35, P = 0.002). Our meta-analysis showed that high levels of ctDNA were associated with poor prognosis in patients with lymphoma, especially DLBCL.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10238-021-00718-8DOI Listing
May 2021

Burden and causes of hospital admissions and readmissions in patients undergoing hemodialysis and peritoneal dialysis: a nationwide study.

J Nephrol 2021 May 13. Epub 2021 May 13.

Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden.

Background: High rates of hospitalization in dialysis patients impose an increasing healthcare burden. We explored and compared hospital admission rates among patients starting hemodialysis (HD) and peritoneal dialysis (PD), and investigated causes of admission/readmission in search of potentially preventable risks.

Methods: Observational study recruiting 8902 patients (3101 on PD) who started maintenance dialysis in Sweden between 2006 and 2016 and were followed-up for 2 years. We compared the Hazard Ratios (HR) for hospital admission and in-hospital death, and calculated the odds ratios (OR) of readmission within 30 days after discharge.

Results: Six thousand four hundred ninety-three (73%) patients were hospitalized at least once, and 246 admissions ended with in-hospital death. Compared with HD, patients on PD had a higher risk of hospitalization (HR 1.07; 95% CI 1.01-1.13), longer length of stay (mean difference of 2.06; 1.39-2.73 days), and higher risk of in-hospital death (HR 1.18; 1.03-1.37). Peritonitis and cardiovascular events were the most frequent causes of admission. Of 5810 patients discharged from the hospital, 1447 (25%) were readmitted and 124 (2%) died within 30 days. No differences in readmission risk were observed between dialysis modalities. There was frequently discordance between the cause of hospital admission and readmission, and we identified a consistent pattern of readmission attributed to complications from infections and their interplay with cardiovascular diseases.

Conclusions: Our study illustrates a high burden of hospitalization in patients on dialysis, suggests the risk of longer hospitalizations for patients on PD, and identifies cardiovascular events and infections as complications that may benefit from closer post-discharge monitoring.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40620-021-01023-zDOI Listing
May 2021

Potent ex vivo armed T cells using recombinant bispecific antibodies for adoptive immunotherapy with reduced cytokine release.

J Immunother Cancer 2021 May;9(5)

Pediatrics, Memorial Sloan Kettering Cancer Center, New York, New York, USA

Background: T cell-based immunotherapies using chimeric antigen receptors (CAR) or bispecific antibodies (BsAb) have produced impressive responses in hematological malignancies. However, major hurdles remained, including cytokine release syndrome, neurotoxicity, on-target off-tumor effects, reliance on autologous T cells, and failure in most solid tumors. BsAb armed T cells offer a safe alternative.

Methods: We generated ex vivo armed T cells (EATs) using IgG-[L]-scFv-platformed BsAb, where the anti-CD3 (huOKT3) scFv was attached to the light chain of a tumor-binding IgG. BsAb density on EAT, in vitro cytotoxicity, cytokine release, in vivo trafficking into tumors, and their antitumor activities were evaluated in multiple cancer cell lines and patient-derived xenograft mouse models. The efficacy of EATs after cryopreservation was studied, and gamma delta (γδ) T cells were investigated as unrelated alternative effector T cells.

Results: The antitumor potency of BsAb armed T cells was substantially improved using the IgG-[L]-scFv BsAb platform. When compared with separate BsAb and T cell injection, EATs released less TNF-α, and infiltrated tumors faster, while achieving robust antitumor responses. The in vivo potency of EAT therapy depended on BsAb dose for arming, EAT cell number per injection, total number of EAT doses, and treatment schedule intensity. The antitumor efficacy of EATs was preserved following cryopreservation, and EATs using γδ T cells were safe and as effective as αβ T cell-EATs.

Conclusions: EATs exerted potent antitumor activities against a broad spectrum of human cancer targets with remarkable safety. The antitumor potency of EATs depended on BsAb dose, cell number and total dose, and schedule. EATs were equally effective after cryopreservation, and the feasibility of third-party γδ-EATs offered an alternative for autologous T cell sources.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/jitc-2020-002222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126293PMC
May 2021

Isolation of sp. nov., a novel bacterium in the family from marine mangrove sediment.

Int J Syst Evol Microbiol 2021 May;71(5)

Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China.

A Gram-stain-negative, strictly aerobic and oval-shaped bacterial strain with a flagellum, designated GS-10, was isolated from mangrove wetland sediment. GS-10 grew at 20-40 °C (optimum, 37 °C), in the pH range of 5.0-11.0 (optimum, 6.0-8.0) and under various NaCl concentrations from 1 to 11 % (w/v) (optimum, 5-6 %). The respiratory quinone was ubiquinone-10, and the predominant polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids (>10 % of the total fatty acids) were summed feature 4 (Ciso I/anteiso B) and summed feature 8 (Cω7 and/or Cω6). The G+C content of the genomic DNA was 63.71 %. On the basis of the results from comparative analysis of the 16S rRNA gene sequence, GS-10 represents a member of the family and had the highest sequence similarity to CECT 4357 (97.47 %), followed by W11-2B (97.03 %), CL-SK44 (96.83 %), CECT 5118 (96.75 %) and CECT 5383 (96.68 %). Phylogenetic trees based on 16S rRNA gene sequences, multilocus sequence analysis (MLSA) and whole genome sequences revealed that GS-10 clustered with species within the genus . The average nucleotide identity (ANI) and the average amino acid identity (AAI) values were calculated from complete genome sequences and indicated that GS-10 represented a novel species of the genus , and the name sp. nov. is proposed for this species. The type strain of is GS-10 (=MCCC 1K03624=KCTC 82131).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004801DOI Listing
May 2021

[Carrier screening model for Duchenne muscular dystrophy for women of reproductive age based on a pre-pregnancy birth defect control platform].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 May;38(5):485-487

Hangzhou Health Service Center for Children and Women, Hangzhou, Zhejiang 310006, China.

Objective: To establish a screening model for females of reproductive age carrying Duchenne muscular dystrophy (DMD) variants based on a current community health examination platform.

Methods: A total of 61 870 participants were recruited between October 2017 and October 2019. Serum creatine kinase (CK) was measured with a Roche Cobasc 701/702 using an enzymatic rate method. Genetic testing was offered to those with a CK level of ≥ 200 U/L. For carriers of DMD variants, genetic counseling and follow up were provided.

Results: For the 61 870 females participating in the program, 1078 were found with raised serum CK (≥ 200 U/L), of which 618 (57.33%) accepted CK re-measurement after at least a two-week interval. One hundred and twenty cases were found with sustained serum CK elevation, of which 6 were confirmed to be definite DMD carriers regardless of family history. Genetic testing was provided to 33 females with a family history for DMD, and 13 were determined as definite carriers. An affected fetus was detected by prenatal diagnosis. After genetic counseling, the parents had opted induced abortion.

Conclusion: Large-scale DMD carrier screening through a three-step approach based on the current community health examination platform is both feasible and cost effective.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3760/cma.j.cn511374-20200331-00223DOI Listing
May 2021

Ubiquitin-mediated DNA damage response is synthetic lethal with G-quadruplex stabilizer CX-5461.

Sci Rep 2021 May 7;11(1):9812. Epub 2021 May 7.

Department of Molecular Oncology, BC Cancer Agency, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada.

CX-5461 is a G-quadruplex (G4) ligand currently in trials with initial indications of clinical activity in cancers with defects in homologous recombination repair. To identify more genetic defects that could sensitize tumors to CX-5461, we tested synthetic lethality for 480 DNA repair and genome maintenance genes to CX-5461, pyridostatin (PDS), a structurally unrelated G4-specific stabilizer, and BMH-21, which binds GC-rich DNA but not G4 structures. We identified multiple members of HRD, Fanconi Anemia pathways, and POLQ, a polymerase with a helicase domain important for G4 structure resolution. Significant synthetic lethality was observed with UBE2N and RNF168, key members of the DNA damage response associated ubiquitin signaling pathway. Loss-of-function of RNF168 and UBE2N resulted in significantly lower cell survival in the presence of CX-5461 and PDS but not BMH-21. RNF168 recruitment and histone ubiquitination increased with CX-5461 treatment, and nuclear ubiquitination response frequently co-localized with G4 structures. Pharmacological inhibition of UBE2N acted synergistically with CX-5461. In conclusion, we have uncovered novel genetic vulnerabilities to CX-5461 with potential significance for patient selection in future clinical trials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-88988-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105411PMC
May 2021

A facile polymer mediated dye incorporation method for fluorescence encoded microbeads with large encoding capacities.

Chem Commun (Camb) 2021 May;57(37):4548-4551

School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.

Here we report a facile dye incorporation method for fluorescence encoded microbeads, which is achieved by tuning the mixed polymer type (blank and dye-labeled polymers) and their doping ratio through electrostatic loading into mesoporous beads. This method is universal to various carriers and could render large encoding capacities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cc08202cDOI Listing
May 2021