Publications by authors named "Hong Xia"

534 Publications

Conducting molybdenum sulfide/graphene oxide/polyvinyl alcohol nanocomposite hydrogel for repairing spinal cord injury.

J Nanobiotechnology 2022 May 6;20(1):210. Epub 2022 May 6.

Guangdong Key Lab of Orthopedic Technology and Implant Materials, Key Laboratory of Trauma & Tissue Repair of Tropical Area of PLA, Orthopedic Center, General Hospital of Southern Theater Command of PLA, Guangzhou, 510010, Guangdong, China.

A sort of composite hydrogel with good biocompatibility, suppleness, high conductivity, and anti-inflammatory activity based on polyvinyl alcohol (PVA) and molybdenum sulfide/graphene oxide (MoS/GO) nanomaterial has been developed for spinal cord injury (SCI) restoration. The developed (MoS/GO/PVA) hydrogel exhibits excellent mechanical properties, outstanding electronic conductivity, and inflammation attenuation activity. It can promote neural stem cells into neurons differentiation as well as inhibit the astrocytes development in vitro. In addition, the composite hydrogel shows a high anti-inflammatory effect. After implantation of the composite hydrogel in mice, it could activate the endogenous regeneration of the spinal cord and inhibit the activation of glial cells in the injured area, thus resulting in the recovery of locomotor function. Overall, our work provides a new sort of hydrogels for SCI reparation, which shows great promise for improving the dilemma in SCI therapy.
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http://dx.doi.org/10.1186/s12951-022-01396-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074236PMC
May 2022

Reversal of hepatorenal syndrome and kidney recovery: When renal has more to offer.

Semin Dial 2022 Apr 22. Epub 2022 Apr 22.

Department of Nephrology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Hangzhou, China.

Hepatorenal syndrome (HRS) is one of the most severe complications in advanced cirrhosis. Type-1 HRS is relatively uncommon, yet carries considerably higher mortality rate. Effective treatment for HRS, especially therapy towards survival benefits, is still limited. However, the role for dialysis in HRS has been questioned over the years. The initiation of dialysis remains controversial for those who aren't transplant candidates. Meanwhile, there's a growing attention towards the successful use of peritoneal dialysis (PD) in cirrhotic patients. Herein, we report a case of HRS-1 in a 76-year-old male patient with decompensated cirrhosis. Through a series of adjustments of hemodialysis regimens and pharmacological prescriptions, patient stabilized and the opportunity for transjugular intrahepatic portosystemic shunt (TIPS) insertion was gained. PD was initiated after TIPS placement. With a gradual decrease of dialysis dose, patient successfully weaned off PD and achieved both reversal of HRS and kidney recovery. Markedly improved nutritional status and quality of life were reported. The potential role of dialysis and TIPS in HRS may be worth revisiting. Further studies regarding the optimal timing of dialysis initiation, choices of dialysis modality, and efficacy of dialysis therapy in combination with TIPS in HRS patients are warranted.
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http://dx.doi.org/10.1111/sdi.13083DOI Listing
April 2022

A dual-mode immunosensing strategy for prostate specific antigen detection: Integration of resonance Raman scattering and photoluminescence properties of ZnS:Mn nanoprobes.

Anal Chim Acta 2022 May 26;1205:339775. Epub 2022 Mar 26.

Key Laboratory of UV-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, Changchun, 130024, PR China. Electronic address:

Luminescence-based methods are widely used for the detection of prostate specific antigen (PSA), for example during the diagnosis of prostate cancer. However, the accuracy of these methods is sub-optimal. The aim of this study was to develop an accurate and sensitive dual-mode immunosensing technique using a combination of resonance Raman scattering (RRS) and photoluminescence (PL) signals for the detection of PSA. A ZnS:Mn nanoprobe was used as the signal reporter, which exhibits both multi-phonon RRS and PL properties. The RRS signal intensity at 348 cm and the PL signal intensity at 590 nm were used for the quantitative assay of PSA. The reproducibility, selectivity and specificity of this dual-mode immunosensing strategy demonstrated an improvement compared with commercial PSA ELISA kits in the analysis of serum samples. Therefore, the RRS-PL immunosensing technique developed in this study shows potential as a reliable technique to be used in the clinical diagnosis of prostate cancer.
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http://dx.doi.org/10.1016/j.aca.2022.339775DOI Listing
May 2022

A novel bone cement injector augments Chinese osteoporotic lumbar pedicle screw channel: a biomechanical investigation.

BMC Musculoskelet Disord 2022 Apr 12;23(1):353. Epub 2022 Apr 12.

Department of Orthopedics, General Hospital of Southern Theater Command of PLA, Guangzhou, 510000, People's Republic of China.

Background: The study aimed to (1) create a series of pedicle injectors with different number of holes on the sheath especially for the Chinese elderly patients and (2) further investigate the effects of the injectors on the augmentation of pedicle screw among osteoporotic lumbar pedicle channel.

Methods: This study used the biomechanical test module of polyurethane (Pacific Research Laboratory Corp, USA) to simulate the mechanical properties of human osteoporotic cancellous bone. The bone cement injectors were invented based on anatomical parameters of lumbar pedicle in Chinese elderly patients. Mechanical test experiments were performed on the bone cement injectors according to the three groups, namely, a local augmentation group, a full-length augmentation group, and a control group. The local augmentation group included three subgroups including 4-hole group, 6-hole group, and 8-hole group. All holes were laterally placed. The full-length augmentation group was a straight-hole injector. The control group was defined that pedicle screws were inserted without any cement augmentation. Six screws were inserted in each group and the maximum insertion torque was recorded. After 24 h of injecting acrylic bone cement, routine X-ray and CT examinations were performed to evaluate the distribution of bone cement. The axial pull-out force of screws was tested with the help of the material testing system 858 (MTS-858) mechanical tester.

Results: The bone cement injectors were consisted of the sheaths and the steel rods and the sheaths had different number of lateral holes. The control group had the lowest maximum insertion torque as compared with the 4-hole, 6-hole, 8-hole, and straight pore groups (P < 0.01), but the differences between the 4-hole, 6-hole, 8-hole, and straight pore groups were no statistical significance. The control group had the lowest maximum axial pull-out force as compared with the other four groups (P < 0.01). Subgroup analysis showed the 8-hole group (161.35 ± 27.17 N) had the lower maximum axial pull-out force as compared with the 4-hole group (217.29 ± 49.68 N), 6-hole group (228.39 ± 57.83 N), and straight pore group (237.55 ± 35.96 N) (P < 0.01). Bone cement was mainly distributed in 1/3 of the distal end of the screw among the 4-hole group, in the middle 1/3 and distal end of the screw among the 6-hole group, in the proximal 1/3 of the screw among the 8-hole group, and along the long axis of the whole screw body in the straight pore group. It might indicate that the 8-hole and straight-hole groups were more vulnerable to spinal canal cement leakage. After pullout, bone cement was also closely connected with the screw without any looseness or fragmentation.

Conclusions: The bone cement injectors with different number of holes can be used to augment the pedicle screw channel. The pedicle screw augmented by the 4-hole or 6-hole sheath may have similar effects to the straight pore injector. However, the 8-hole injector may result in relatively lower pull-out strength and the straight pore injector has the risks of cement leakage as well as cement solidarization near the screw head.
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http://dx.doi.org/10.1186/s12891-022-05181-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004128PMC
April 2022

Developing a novel calcium magnesium silicate/graphene oxide incorporated silk fibroin porous scaffold with enhanced osteogenesis, angiogenesis and inhibited osteoclastogenesis.

Biomed Mater 2022 May 3;17(3). Epub 2022 May 3.

Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, Guangzhou, 510010, People's Republic of China.

Recently, biofunctional ions (Mg, Si, etc) and graphene derivatives are proved to be promising in stimulating bone formation. In this study, a novel inorganic/organic composite porous scaffold based on silk fibroin (SF), graphene oxide (GO), and calcium magnesium silicate (CMS) was developed for bone repair. The porous scaffolds obtained by lyophilization showed a little difference in pore structure while GO and CMS displayed a good interaction with SF matrix. The addition of CMS with good mineralization potential and sustainedly release ability of biofunctional ions (Ca, Mgand Si) increased the strength of SF scaffolds a little and facilitated the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) by upregulating bone formation-related genes (ALP, COL1, OC and Runx2). The further incorporation of GO in SF scaffolds enhanced the compressive strength and water retention, and also remarkably promoted the osteogenic differentiation of BMSCs. Besides, the angiogenesis of human umbilical vein endothelial cells was significantly promoted by CMS/GO/SF scaffold extract through the upregulation of angiogenesis genes (eNOs and bFGF). Moreover, the osteoclastic formation ability of RAW264.7 cells was suppressed by the released ions from CMS/GO/SF scaffold through the down-regulation of CAK, MMP9 and TRAP. The promoted osteogenesis, angiogenesis and inhibited osteoclastogenesis functions of CMS/GO/SF composite scaffold may enable it as a novel therapy for bone repair and regeneration.
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http://dx.doi.org/10.1088/1748-605X/ac65ccDOI Listing
May 2022

Oridonin Relieves Angiotensin II-Induced Cardiac Remodeling via Inhibiting GSDMD-Mediated Inflammation.

Cardiovasc Ther 2022 14;2022:3167959. Epub 2022 Mar 14.

The Key Laboratory of Cardiovascular Disease of Wenzhou, Department of Cardiac Care Unit, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Myocardial remodeling is one of the main lesions in the late stage of chronic heart failure and seriously affects the prognosis of patients. Continuous activation of the renin-angiotensin-aldosterone system (RAAS) contributes to the development of myocardial remodeling greatly, and angiotensin II (Ang II), its main constituent, can directly lead to cardiac remodeling through an inflammatory response and oxidative stress. Since Ang II-induced myocardial remodeling is closely related to inflammation, we tried to explore whether the anti-inflammatory drug oridonin (Ori) can reverse this process and its possible mechanism. Our study investigated that hypertrophy and fibrosis can be induced after being treated with Ang II in cardiomyocytes (H9c2 cells and primary rat cardiomyocytes) and C57BL/6J mice. The anti-inflammatory drug oridonin could effectively attenuate the degree of cardiac remodeling both in vivo and vitro by inhibiting GSDMD, a key protein of intracellular inflammation which can further activate kinds of inflammation factors such as IL-1 and IL-18. We illustrated that oridonin reversed cardiac remodeling by inhibiting the process of inflammatory signaling through GSDMD. After inhibiting the expression of GSDMD in cardiomyocytes by siRNA, it was found that Ang II-induced hypertrophy was attenuated. These results suggest that oridonin is proved to be a potential protective drug against GSDMD-mediated inflammation and myocardial remodeling.
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http://dx.doi.org/10.1155/2022/3167959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938085PMC
April 2022

Comprehensive analysis of expression and prognosis for LMNB family genes in human sarcoma.

Medicine (Baltimore) 2022 Mar;101(11)

Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China,Department of Cardiology, The Fourth Hospital ofChangsha, Changsha, Hunan, China,Department of Spine Surgery, Longhui People's Hospital, Shaoyang, Hunan, China,Department of Orthopedics, The FirstPeople's Hospital of Changde City, Changde, Hunan, China,Department of Emergency, The First Hospital of Changsha City, Changsha, Hunan, China,Departmentof Orthopedics, Xiangtan Central Hospital, Xiangtan, Hunan, China.

Abstract: Previous studies indicated that lamin proteins were thought to be related to gene expression, chromatin structure, and unclear stability. There are 2 types of vertebrate lamins, including A and B. The 2 B type proteins are encoded by lamin B1 (LMNB1) and lamin B2 (LMNB2). The LMNBs factor has been found to be associated with the development of multiple tumors, but its association with sarcoma has been barely mentioned.The transcription levels of LMNBs were analyzed via Oncomine database. Gene Expression Profiling Interactive Analysis (GEPIA) dataset was adopted to analyze the differential expression of LMNBs in sarcoma. Cancer Cell Line Encyclopedia dataset was used to explore the expression of LMNBs in sarcoma cell line. We analyzed the prognostic value of LMNBs in GEPIA and Kaplan-Meier Plotter. Oncomine and GEPIA datasets were also used to detect the relationship between LMNBs and their co-expressed genes. We used the Database for Annotation, Visualization and Integrated Discovery to conduct the Gene Ontology analysis of LMNBs and their co-expressed genes. Kyoto Encyclopedia of Genes and Genomes was also used to analyze the pathway of LMNBs.LMNB1 and LMNB2 were reported to be hyperexpressed in sarcoma. The expression of LMNBs was elevated in various sarcoma cell lines. According to the results, we observed that LMNBs were connected to the poor overall survival, recurrence-free survival, and disease-free survival of sarcoma patients.This study indicated that hyperexpression of LMNBs was significantly related to worse outcome of sarcoma, LMNB1 and LMNB2 were expected to become potential biomarkers for human.
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http://dx.doi.org/10.1097/MD.0000000000028933DOI Listing
March 2022

The effect of anteromedial support plate with three cannulated screws in the treatment of Pauwels type III femoral neck fracture in young adults.

Eur J Trauma Emerg Surg 2022 Mar 15. Epub 2022 Mar 15.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou, 510515, Guangdong, People's Republic of China.

Purpose: This study is aimed to evaluate the clinical effects of anteromedial support plate combined with three cannulated screws on Pauwels type III femoral neck fractures in young adults.

Methods: We conducted a retrospective study of 27 patients with Pauwels type III femoral neck fracture treated by anteromedial support plate combined with three cannulated screws. The results of fracture healing and complications for all patients were assessed by postoperative imaging examination. The Harris hip score was used to evaluate the functional outcome of the hip at the last follow-up.

Results: The fracture union was achieved in 25 patients (92.6%). However, two patients required reoperation (two for nonunion). Early implant failure occurred in one patient, neck shortening in one patient and coxa vara in one patient. The excellent or good outcome of the Harris hip score was 96% for the 25 patients with satisfactory fracture union.

Conclusion: Our preliminary results indicate that anteromedial support plate combined with three cannulated screws is a good alternative in the treatment of Pauwels type III femoral neck fractures in young adults with high union rate and low incidence of complications including nonunion and avascular necrosis of the femoral head.
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http://dx.doi.org/10.1007/s00068-022-01931-0DOI Listing
March 2022

Long non-coding RNA ANRIL serves as a potential marker of disease risk, inflammation, and disease activity of pediatric inflammatory bowel disease.

Clin Res Hepatol Gastroenterol 2022 Feb 25;46(4):101895. Epub 2022 Feb 25.

Department of Pediatrics, The Second Affiliated Hospital of Baotou Medical College, Baotou, China.

Background: Long non-coding antisense RNAs in the INK4 locus (lnc-ANRIL) have been reported to be involved in inflammation and immunity. However, few studies have reported its clinical application in pediatric inflammatory bowel disease (IBD). Therefore, we conducted this study to investigate the correlation between lnc-ANRIL expression and disease risk, inflammation, and activity in pediatric IBD patients.

Methods: Pediatric patients with Crohn's disease (CD; n = 40), ulcerative colitis (UC; n = 40), and controls (n = 20) were recruited. For all pediatric IBD patients, lnc-ANRIL expression in peripheral blood mononuclear cells and serum inflammatory cytokine levels were measured by RT-qPCR and ELISA, respectively. For the controls, lnc-ANRIL expression was also measured.

Results: Lnc-ANRIL levels were lower in CD (P = 0.002) and UC (P = 0.001) patients compared with the controls; negatively correlated with C-reactive protein levels (P<0.01), erythrocyte sedimentation rate (P<0.01), disease activity (P<0.05), and severity (P<0.05) in CD and UC patients; and inversely associated with tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-17, and IL-23 levels in both CD and UC patients (all P<0.01). Further subgroup analyses revealed that the association between lnc-ANRIL and inflammatory cytokines and disease activity was more remarkable in pediatric patients with moderate or severe IBD.

Conclusion: Lnc-ANRIL may serve as a potential marker for evaluating disease risk and monitoring disease activity in pediatric IBD patients.
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http://dx.doi.org/10.1016/j.clinre.2022.101895DOI Listing
February 2022

Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy.

Front Genet 2022 8;13:781676. Epub 2022 Feb 8.

The Key Laboratory of Cardiovascular Disease of Wenzhou, Department of Cardiology, The First Affiliated Hospital of WenZhou Medical University, WenZhou, China.

Cardiac hypertrophy is an adaptive cardiac response that accommodates the variable hemodynamic demands of the human body during extended periods of preload or afterload increase. In recent years, an increasing number of studies have pointed to a potential connection between myocardial hypertrophy and abnormal expression of non-coding RNAs. Circular RNA (circRNA), as one of the non-coding RNAs, plays an essential role in cardiac hypertrophy. However, few studies have systematically analyzed circRNA-related competing endogenous RNA (ceRNA) regulatory networks associated with cardiac hypertrophy. Therefore, we used public databases from online prediction websites to predict and screen differentially expressed mRNAs and miRNAs and ultimately obtained circRNAs related to cardiac hypertrophy. Based on this result, we went on to establish a circRNAs-related ceRNA regulatory network. This study is the first to establish a circRNA-mediated ceRNA regulatory network associated with myocardial hypertrophy. To verify the results of our analysis, we used PCR to verify the differentially expressed mRNAs and miRNAs in animal myocardial hypertrophy model samples. Our findings suggest that three mRNAs (Col12a1, Thbs1, and Tgfbr3), four miRNAs (miR-20a-5p, miR-27b-3p, miR-342-3p, and miR-378a-3p), and four related circRNAs (circ_0002702, circ_0110609, circ_0013751, and circ_0047959) may play a key role in cardiac hypertrophy.
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http://dx.doi.org/10.3389/fgene.2022.781676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860901PMC
February 2022

Clogging and avalanches in quasi-two-dimensional emulsion hopper flow.

Phys Rev E 2022 Jan;105(1-1):014603

Department of Physics, Emory University, Atlanta, Georgia 30322, USA.

We experimentally and computationally study the flow of a quasi-two-dimensional emulsion through a constricting hopper shape. Our area fractions are above jamming such that the droplets are always in contact with one another and are in many cases highly deformed. At the lowest flow rates, the droplets often clog and thus exit the hopper via intermittent avalanches. At the highest flow rates, the droplets exit continuously. The transition between these two types of behaviors is a fairly smooth function of the mean strain rate. The avalanches are characterized by a power-law distribution of the time interval between droplets exiting the hopper, with long intervals between the avalanches. Our computational studies reproduce the experimental observations by adding a flexible compliance to the system (in other words, a finite stiffness of the sample chamber). The compliance results in continuous flow at high flow rates, and allows the system to clog at low flow rates leading to avalanches. The computational results suggest that the interplay of the flow rate and compliance controls the presence or absence of the avalanches.
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http://dx.doi.org/10.1103/PhysRevE.105.014603DOI Listing
January 2022

Smart Diffraction Gratings Based on the Shape Memory Effect.

Macromol Rapid Commun 2022 Apr 25;43(7):e2100863. Epub 2022 Feb 25.

State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.

The shape memory effect is the capability of a structure or a material that can be deformed into a certain temporary shape under external stimulus, and the shape will be fixed without the stimulus. The recovery process can be triggered by the same stimulus. The intelligent tunable device based on the shape memory effect has a wide range of applications in many fields. In the optical field, smart diffraction gratings can accomplish in situ optical diffractions according to requirements, meeting the high demand in the next generation of smart optical systems. However, it is essential to construct high-precision grating structures based on shape memory materials. Here, a smart diffraction grating based on UV-curable shape memory polymers (SMPs) via two-beam interference is reported, with nano-scale precision, excellent deformability and recovery ability, and adjustable spectroscopic performance. More importantly, based on the shape memory effect, grating structures that surpass the precision of the fabrication system can be obtained. The smart grating exhibits rapid deformation and recovery upon heating and long-term storage capability, which facilitates them to be applied in optics, electronics, and integrated sensing.
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http://dx.doi.org/10.1002/marc.202100863DOI Listing
April 2022

Cross-Cultural Adaptation and Validation of the Chinese Version of the Brace Questionnaire.

Front Pediatr 2021 13;9:763811. Epub 2022 Jan 13.

Department of Orthopedics, People's Liberation Army General Hospital of Southern Theater Command, Guangzhou, China.

To adapt the questionnaire cross-culturally and to analyze the adaptation and validation of the Chinese version of the Brace Questionnaire (C-BrQ). The adaptation was based on the International Quality of Life Assessment Project guidelines. A total of 79 patients with AIS were included to examine the psychometric properties of the C-BrQ. The reliability was assessed using internal consistency (the Cronbach's alpha coefficient) and test-retest reliability (intraclass correlation coefficient ICC, 95% CI). Floor and ceiling effects were calculated. Lin's concordance correlation coefficient (CCC, 95% CI) was used to compare the agreement between the Scoliosis Research Society-22 patient questionnaire (SRS-22) and C-BrQ. There were strong correlations between each item and its corresponding domain significantly. The correlations between the C-BrQ domains and their related questions vary from moderate to strong ( = 0.311-0.933, < 0.05). The Cronbach's was 0.891, showing good internal consistency of each domain of the BrQ, and the ICC in test-retest was 0.860 (0.8776, 0.912), which means an excellent test-retest reliability. The Lin's CCC between SRS-22 and C-BrQ was 0.773 (0.669, 0.848), showing great agreement. However, no significant floor and ceiling effects in C-BrQ was observed except the ceiling effect in school activity and bodily pain. BrQ was translated and cross-culturally adapted for use in China with good internal consistency and excellent test-retest reliability.
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http://dx.doi.org/10.3389/fped.2021.763811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8793733PMC
January 2022

"Off-On" typed upconversion fluorescence resonance energy transfer probe for the determination of Cu in tap water.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Apr 20;271:120920. Epub 2022 Jan 20.

Key Laboratory of UV-Emitting Materials and Technology at Ministry of Education, Northeast Normal University, Changchun 130024, PR China. Electronic address:

Detection of copper plays a prominent role in the environmental protection and human health. Herein, we firstly design and construct an "off-on" upconversion fluorescence resonance energy transfer (UFRET) probe with low toxicity for the Cu determination by using NaYF: Yb, Er upconversion nanoparticles (UCNPs) and Au NPs. UCNPs with positive charge and Au NPs with negative charge are respectively employed as the donor and acceptor, and bound together to form UFRET probe. The upconversion fluorescence quenching of UCNPs occurs by Au NPs through FRET (defined as "off" state). When Cu exists in samples, Cu reacts with 4-mercaptobenzoic acid (4-MBA) capped on the surface of Au NPs to make Au NPs detach from UCNPs, leading to the termination of FRET and the recovery of upconversion fluorescence (defined as "on" state). "Off-on" typed UFRET probe has excellent sensing performances, including linear range of 0.02-1 μM Cu concentration, the limit of detection of 18.2 nM, high selectivity to Cu and good recovery. The probe has been successfully used to determine Cu in spiked tap water with satisfactory results. The probe will provide theoretical and technical support for the design of new sensitive heavy metal ion detection probe.
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http://dx.doi.org/10.1016/j.saa.2022.120920DOI Listing
April 2022

Butyl Benzyl Phthalate in Urban Sewage by Magnetic-Based Immunoassay: Environmental Levels and Risk Assessment.

Biosensors (Basel) 2022 Jan 15;12(1). Epub 2022 Jan 15.

Institute of Environmental Health and Ecological Security, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.

A magnetic-based immunoassay (MBI) combined with biotin-streptavidin amplification was proposed for butyl benzyl phthalate (BBP) investigation and risk assessment. The values of LOD (limit of detection, IC) and IC were 0.57 ng/mL and 119.61 ng/mL, with a detection range of 0.57-24,977.71 ng/mL for MBI. The specificity, accuracy and precision are well demonstrated. A total of 36 environmental water samples of urban sewage from Zhenjiang, China, were collected and assessed for BBP contamination. The results show that BBP-positive levels ranged from 2.47 to 89.21 ng/mL, with a positive rate of 77.8%. The health effects of BBP in the urban sewage were within a controllable range, and the ambient severity for health (ASI) was below 1.49. The highest value of AS for ecology (ASII) was 7.43, which indicates a potential harm to ecology. The entropy value of risk quotient was below 100, the highest being 59.47, which poses a low risk to the environment and ecology, indicating that there is a need to strengthen BBP controls. The non-carcinogenic risk of BBP exposure from drinking water was higher for females than that for males, and the non-carcinogenic risk from drinking-water and bathing pathways was negligible. This study could provide an alternative method for detecting BBP and essential information for controlling BBP contamination.
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http://dx.doi.org/10.3390/bios12010045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773763PMC
January 2022

An injectable superior depot of Telratolimod inhibits post-surgical tumor recurrence and distant metastases.

Acta Biomater 2022 03 13;141:132-139. Epub 2022 Jan 13.

Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address:

The clinical success of Toll-like receptor (TLR) agonists is based on their capacity to efficiently mobilize both innate and adaptive immunity. However, rapid distribution of TLR agonists into the systemic circulation may result in systemic cytokine storms. Telratolimod (Tel) is a TLR 7/8 agonist whose structure has a hydrophobic long chain that helps to prolong its release. Despite this, the phase I study of Tel showed cytokine release syndromes in 3/35 patients. Herein, we designed an injectable phase transition gel (PGE) that served as a superior drug depot for fatty acid-modified drugs. PGE further minimized the systemic drug exposure of Tel and the possible cytokine storms. In vivo studies demonstrated that [email protected] facilitated the recruitment of effector CD8 T lymphocytes (T cells) and the polarization of myeloid-derived suppressor cells (MDSCs) and immunosuppressive M2-like macrophages to tumoricidal antigen-presenting cells. The reshaping of the tumor microenvironment (TME) by [email protected] elicited systematic immune responses to significantly prevent B16F10 or 4T-1 tumor postoperative recurrence and metastasis. Therefore, this platform of Tel is expected to provide a clinically available option for effective postoperative combined therapy. STATEMENT OF SIGNIFICANCE: A series of prodrugs or conjugates containing hydrophobic blocks were designed to achieve sustained release at the injection site by reducing the water solubility. However, this strategy sometimes failed short of expectations. Thus, we constructed a biocompatible and biodegradable injectable phase transition gel (PGE) with superior release properties that can be injected subcutaneously into the surgery site. In the long-lasting treatment, the melanoma and breast cancer immunotherapeutic effect significantly enhanced and the risk of cancer metastasis and relapse was reduced. Crucially, for some immune agonists, a superior release control can significantly reduce adverse effects which was decisive for the availability of the drugs.
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http://dx.doi.org/10.1016/j.actbio.2022.01.013DOI Listing
March 2022

Circ_SEC61A1 contributes to the progression of multiple myeloma cells via regulating miR-660-5p/CDK6 axis.

Leuk Res 2022 02 31;113:106774. Epub 2021 Dec 31.

Epartment of Orthopedics, Central Hospital of XiangTan, China. Electronic address:

Background: Circular RNAs (circRNAs) have been reported to play critical roles in the malignant progression of diverse human cancers, including multiple myeloma (MM). This study aimed to explore the functional role and underlying mechanism of circ_SEC61A1 in MM progression.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed to detect the expression levels of circ_SEC61A1, microRNA (miR)-660-5p and cyclin-dependent kinase 6 (CDK6) mRNA. The localization of circ_SEC61A1 in MM cells was tested by the subcellular fractionation location assay. Actinomycin D assay was conducted to determine the characteristics of circ_SEC61A1. Cell proliferation was evaluated by colony formation assay, 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay and 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Western blot assay was exploited to examine the expression of proteins. Cell migration and invasion were tested via transwell assay, and cell apoptosis was measured by flow cytometry analysis. Dual-luciferase reporter assay was utilized to confirm the interaction between miR-660-5p and circ_SEC61A1 or CDK6.

Results: Circ_SEC61A1 level was increased in MM tissues and cells. Circ_SEC61A1 was a stable circRNA and mainly located in cytoplasm. Circ_SEC61A1 silence restrained the proliferation, metastasis and expedited the apoptosis in MM cells. CDK6 was identified as the target of miR-660-5p, and circ_SEC61A1 sponged miR-660-5p to positively regulate CDK6 expression. The inhibitory impacts of circ_SEC61A1 knockdown on the progression of MM cells were mitigated by miR-660-5p inhibition. MiR-660-5p overexpression blocked the malignant phenotypes of MM cells by targeting CDK6.

Conclusion: Our study manifested that circ_SEC61A1 could accelerate MM progression at least partially through modulating miR-660-5p/CDK6 axis.
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http://dx.doi.org/10.1016/j.leukres.2021.106774DOI Listing
February 2022

High-resolution patterning of perovskite quantum dots femtosecond laser direct writing.

Nanoscale 2022 Jan 27;14(4):1174-1178. Epub 2022 Jan 27.

State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun 130012, China.

Colloidal quantum dots (QDs) have exhibited great potential for optoelectronic applications, including displays, lasers, anti-counterfeiting and information storage. However, the high-resolution patterning technique of QDs is still a challenge, while precise patterned QDs are of great value for practical applications. Here, a femtosecond laser direct writing strategy was demonstrated for the fabrication of high-resolution-patterned perovskite quantum dots (PQDs) by the laser-induced Marangoni flow to aggregate and deposit the PQDs based on the opto-thermoelectric mechanism. By regulating the laser power and the exposure time, the minimum line width could reach 1.58 μm. Importantly, through the patterning of red, green and blue PQDs, the strategy exhibited the applicability in full-color PQD materials. Moreover, the deposited PQDs can preserve the original photophysical properties including photoluminescence spectra and excited state lifetime. The approach provides a strategy to fabricate high-resolution patterned PQDs , which is a promising alternative in photonic applications including high-resolution displays and anti-counterfeiting.
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http://dx.doi.org/10.1039/d1nr07516kDOI Listing
January 2022

Novel and Variants Identified in Two Unrelated Han-Chinese Patients With Clinically Suspected Brugada Syndrome.

Front Cardiovasc Med 2021 8;8:758903. Epub 2021 Dec 8.

Center for Experimental Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.

Brugada syndrome (BrS) is a complexly genetically patterned, rare, malignant, life-threatening arrhythmia disorder. It is autosomal dominant in most cases and characterized by identifiable electrocardiographic patterns, recurrent syncope, nocturnal agonal respiration, and other symptoms, including sudden cardiac death. Over the last 2 decades, a great number of variants have been identified in more than 36 pathogenic or susceptibility genes associated with BrS. The present study used the combined method of whole exome sequencing and Sanger sequencing to identify pathogenic variants in two unrelated Han-Chinese patients with clinically suspected BrS. Minigene splicing assay was used to evaluate the effects of the splicing variant. A novel heterozygous splicing variant c.2437-2A>C in the sodium voltage-gated channel alpha subunit 5 gene () and a novel heterozygous missense variant c.161A>T [p.(Asp54Val)] in the glycerol-3-phosphate dehydrogenase 1 like gene () were identified in these two patients with BrS-1 and possible BrS-2, respectively. Minigene splicing assay indicated the deletion of 15 and 141 nucleotides in exon 16, resulting in critical amino acid deletions. These findings expand the variant spectrum of and , which can be beneficial to genetic counseling and prenatal diagnosis.
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http://dx.doi.org/10.3389/fcvm.2021.758903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692717PMC
December 2021

Pancreatic and gut hormone responses to mixed meal test in post-chronic pancreatitis diabetes mellitus.

Diabetes Metab 2021 Dec 17;48(3):101316. Epub 2021 Dec 17.

Department of Endocrinology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China; Institute of Pancreas, Southeast University, Nanjing, China. Electronic address:

Objective: More than one-third of chronic pancreatitis patients will eventually develop diabetes, recently classified as post-chronic pancreatitis diabetes mellitus (PPDM-C). This study was aimed to investigate the pancreatic and gut hormone responses to a mixed meal test in PPDM-C patients, compared with non-diabetic chronic pancreatitis (CP), and type 2 diabetes patients or healthy controls.

Design And Methods: Sixteen patients with PPDM-C, 12 with non-diabetic CP as well as 10 with type 2 diabetes and healthy controls were recruited. All participants underwent mixed meal tests, and blood samples were collected for measurements of blood glucose, C-peptide, insulin, glucagon, pancreatic polypeptide (PP), ghrelin, peptide YY, glucagon like peptide-1 (GLP-1) and gastric inhibitory peptide (GIP). Indices of insulin sensitivity and secretion were calculated. Repeated measures analysis of variance was performed.

Results: Participants with PPDM-C exhibited decreases in both fasting and postprandial responses of C-peptide (P < 0.001), insulin (P < 0.001), ghrelin (P < 0.001) and PYY (P = 0.006) compared to participants with type 2 diabetes and healthy controls. Patients with CP showed blunted glucagon, PP and incretin reactions, while the responses were increased in patients with PPDM-C compared to controls. The level of insulin sensitivity was higher for PPDM-C than type 2 diabetes (P < 0.01), however the indices for early/late-phase and overall insulin secretion (P < 0.01) were lower.

Conclusions: Patients with PPDM-C are characterized by decreased C-peptide, insulin, ghrelin and PYY responses, and similar levels of glucagon, PP, GIP and GLP-1 compared to those with type 2 diabetes. The above findings, when confirmed in a larger population, may prove helpful to establish the diagnosis of PPDM-C, and should promote study on underlying pathophysiological mechanisms.
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http://dx.doi.org/10.1016/j.diabet.2021.101316DOI Listing
December 2021

Expression signature, prognosis value and immune characteristics of cathepsin F in non-small cell lung cancer identified by bioinformatics assessment.

BMC Pulm Med 2021 Dec 20;21(1):420. Epub 2021 Dec 20.

Department of Medical Biology, School of Basic Medical Science, Hubei University of Medicine, Shiyan, 442000, Hubei Province, China.

Background: In recent years, immunotherapies and targeted therapies contribute to population-level improvement in NSCLC cancer-specific survival, however, the two novel therapeutic options have mainly benefit patients containing mutated driven genes. Thus, to explore other potential genes related with immunity or targeted therapies may provide novel options to improve survival of lung cancer patients without mutated driven genes. CTSF is unique in human cysteine proteinases. Presently, CTSF has been detected in several cell lines of lung cancer, but its role in progression and prognosis of lung cancer remains unclear.

Methods: CTSF expression and clinical datasets of lung cancer patients were obtained from GTEx, TIMER, CCLE, THPA, and TCGA, respectively. Association of CTSF expression with clinicopathological parameters and prognosis of lung cancer patients was analyzed using UALCAN and Kaplan-Meier Plotter, respectively. LinkedOmics were used to analyze correlation between CTSF and CTSF co-expressed genes. Protein-protein interaction and gene-gene interaction were analyzed using STRING and GeneMANIA, respectively. Association of CTSF with molecular markers of immune cells and immunomodulators was analyzed with Immunedeconv and TISIDB, respectively.

Results: CTSF expression was currently only available for patients with NSCLC. Compared to normal tissues, CTSF was downregulated in NSCLC samples and high expressed CTSF was correlated with favorable prognosis of NSCLC. Additionally, CTSF expression was correlated with that of immune cell molecular markers and immunomodulators both in LUAD and LUSC. Noticeably, high expression of CTSF-related CTLA-4 was found to be associated with better OS of LUAD patients. Increased expression of CTSF-related LAG-3 was related with poor prognosis of LUAD patients while there was no association between CTSF-related PD-1/PD-L1 and prognosis of LUAD patients. Moreover, increased expression of CTSF-related CD27 was related with poor prognosis of LUAD patients while favorable prognosis of LUSC patients.

Conclusions: CTSF might play an anti-tumor effect via regulating immune response of NSCLC.
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http://dx.doi.org/10.1186/s12890-021-01796-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686609PMC
December 2021

Nitride perovskite becomes polar.

Authors:
Xia Hong

Science 2021 Dec 16;374(6574):1445-1446. Epub 2021 Dec 16.

Department of Physics and Astronomy, University of Nebraska-Lincoln, Lincoln, NE 68588-0299, USA.

An oxygen-free polar perovskite offers several advantages over perovskite oxides.
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http://dx.doi.org/10.1126/science.abm7179DOI Listing
December 2021

Programmable fabrication of a miniaturized photodetector with thermal stability via femtosecond laser direct writing.

Opt Lett 2021 Dec;46(24):6075-6078

With the ever-increasing sophistication of integration of electronic devices, the problem of heat accumulation has become ever more serious. Here, a miniaturized photodetector with thermal stability was fabricated by combining the excellent characteristics of femtosecond laser direct writing (FsLDW) and silicon (Si). The sensing part of the photodetector is a Si microwire composed of Si nanoparticles and the sensing area is only 300 μm. As a result, the photodetector can work stably at a temperature as high as 100°C and the response speed of the photodetector becomes notably faster at high temperatures. Furthermore, an image sensor was successfully fabricated by integrating 16 photodetectors and the image sensor can also work stably at high temperatures. This work demonstrates the potential for application of photodetectors based on Si microwires prepared by FsLDW under harsh conditions.
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http://dx.doi.org/10.1364/OL.446556DOI Listing
December 2021

Finite Element Analysis of Horizontal Screw-Screw Crosslink Used in C1-C2 Pedicle Screw-Rod Fixation.

Med Sci Monit 2021 Dec 14;27:e932026. Epub 2021 Dec 14.

The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, China (mainland).

BACKGROUND In the craniocervical junction, a C1-C2 pedicle screw-rod (PSR) fixation is applied to provide stability. The horizontal rod-rod crosslink (hR-R CL) is often used to enhance segmental posterior instrumentation. However, the biomechanics of the alternative horizontal screw-screw crosslink (hS-S CL) in the craniocervical junction are unclear. MATERIAL AND METHODS A nonlinear atlantoaxial instability 3-dimensional C1-C2 finite element model was constructed using computed tomography images. On this basis, 2 fixation models were established with C1-C2 PSR fixation using (1) a rod-rod crosslink (R-R CL), and (2) a screw-screw crosslink (S-S CL). Range of motion (ROM) of the atlantoaxial joint, stress distribution of the implants, and maximum stress value of the vertebral bodies were calculated and compared under 4 loading conditions, including flexion, extension, lateral bending, and axial rotation. RESULTS Atlantoaxial joint ROM was reduced by 90.19% to 98.5% with the hR-R CL, and by 90.1% to 98.7% with the hS-S CL, compared with the instability model. During axial rotation, the total stress peak of the PSR fixation was smaller with hS-S CL than with hR-R CL. The peak stress values of the vertebral bodies were comparable between the 2 fixation models. CONCLUSIONS The 2 tested crosslink models provided comparable stability. However, during axial rotation, the total stress peak of hS-S CL fixation was smaller than that of hR-R CL fixation. Since the atlantoaxial joint primarily functions as a rotational joint, our results suggested that the use of hS-S CL can provide a more stable environment for the implants.
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http://dx.doi.org/10.12659/MSM.932026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684241PMC
December 2021

Predicting outcomes of phase III oncology trials with Bayesian mediation modeling of tumor response.

Stat Med 2022 02 9;41(4):751-768. Epub 2021 Dec 9.

Dell Medical School, The University of Texas at Austin, Austin, Texas, USA.

Pivotal cancer trials often fail to yield evidence in support of new therapies thought to offer promising alternatives to standards-of-care. Conducting randomized controlled trials in oncology tends to be considerably more expensive than studies of other diseases with comparable sample size. Moreover, phase III trial design often takes place with a paucity of survival data for experimental therapies. Experts have explained the failures on the basis of design flaws which produce studies with unrealistic expectations. This article presents a framework for predicting outcomes of phase III oncology trials using Bayesian mediation models. Predictions, which arise from interim analyses, derive from multivariate modeling of the relationships among treatment, tumor response, and their conjoint effects on survival. Acting as a safeguard against inaccurate pre-trial design assumptions, the methodology may better facilitate rapid closure of negative studies. Additionally the models can be used to inform re-estimations of sample size for under-powered trials that demonstrate survival benefit via tumor response mediation. The methods are applied to predict the outcomes of two colorectal cancer studies. Simulation is used to evaluate and compare models in the absence versus presence of reliable surrogate markers of survival.
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http://dx.doi.org/10.1002/sim.9268DOI Listing
February 2022

Electronic band structure of iridates.

Mater Horiz 2021 Aug 25;8(8):2151-2168. Epub 2021 May 25.

Department of Physics and Astronomy, Nebraska Center for Materials and Nanoscience, Theodore Jorgensen Hall, University of Nebraska, 855 N 16th, P. O. Box 880299, Lincoln, Nebraska 68588-0299, USA.

In this review, an attempt has been made to compare the electronic structures of various 5d iridates (iridium oxides), with an effort to note the common features and differences. Both experimental studies, especially angle-resolved photoemission spectroscopy (ARPES) results, and first-principles band structure calculations have been discussed. This brings to focus the fact that the electronic structures and magnetic properties of the high-Z 5d transition iridates depend on the intricate interplay of strong electron correlation, strong (relativistic) spin-orbit coupling, lattice distortion, and the dimensionality of the system. For example, in the thin film limit, SrIrO exhibits a metal-insulator transition that corresponds to the dimensionality crossover, with the band structure resembling that of bulk SrIrO.
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http://dx.doi.org/10.1039/d1mh00063bDOI Listing
August 2021

[Determination of bongkrekic acid in tremella and auricularia auricular by improved QuEChERS method combined with ultra-high performance liquid chromatography-triple quadrupole mass spectrometry].

Se Pu 2021 Dec;39(12):1368-1373

An improved QuEChERS (quick, easy, cheap, effective, rugged, safe) method, combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), was used to determine bongkrekic acid (BA) in tremella and auricularia auricular. BA is a fat-soluble, fatal bacterial toxin produced by the aerobic gram-negative bacteria Burkholderia gladioli pathovar cocovenenans. Tremella and auricularia auricular, which have rich nutritional values, are traditional edible fungi in China that are very popular among Chinese consumers. However, tremella and auricularia auricular are easily contaminated by BA during storage and foaming, and this poses a great threat to food safety and human health. Therefore, establishing a rapid and efficient analysis method for detecting BA in tremella and auricularia auricular is of considerable significance. However, the BA concentration in the actual sample is very low, and the matrices of tremella fuciformis and auricularia auricular are very complex. Thus, it is necessary to employ appropriate sample pretreatment technology to extract and purify BA from tremella and auricularia auricular samples prior to instrumental analysis. In this study, the QuEChERS method, combined with UHPLC-MS/MS, was used to detect BA in tremella and auricularia auricular. The key parameters, such as extraction solvent, extraction method, and adsorbent used for cleanup, were optimized to obtain high extraction efficiency. The content of acetic acid in the extraction solution strongly influenced the extraction efficiency of BA, and acetonitrile with 5%(v/v) acetic acid was determined to be the optimum extraction solvent. After salting out, the acetonitrile extract was purified by dispersive solid phase extraction using 200 mg C18 as a cleanup adsorbent. The sample was then separated on a Waters HSS T3 column (100 mm×2.1 mm, 1.8 μm), using a water solution containing 0.01% (v/v) formic acid and 0.05% (v/v) ammonia and methanol as mobile phases. MS analysis was performed using an electrospray ionization source in the negative and multiple reaction monitoring (MRM) modes. Under the optimized conditions, the matrix effects of UHPLC-MS/MS in tremella and auricularia auricular were -6.3% and -11.5%, respectively; this indicated that the method had a significant purification effect, and the sample matrix did not affect the MS detection of BA. Further study showed that in the concentration range of 1-200 μg/L, the square of the regression coefficient of the linear equation () was greater than 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.15 μg/kg and 0.5 μg/kg, respectively. The average recoveries in samples spiked with 0.5, 10, and 50 μg/kg BA in tremella ranged from 92.4% to 102.6%, and the intra-day and inter-day relative standard deviations (RSDs) were 4.3%-4.9% and 3.2%-3.5%, respectively. For auricularia auricular, the average recoveries ranged from 89.6% to 102.3%, and the intra-day and inter-day RSDs were 2.4%-9.5% and 3.6%-4.1%, respectively. These results indicate that the proposed method has satisfactory sensitivity, accuracy, and precision. Finally, the method showed good performance when applied to the analysis of real samples. Compared with other reported methods, the LOD and LOQ of our proposed method were lower, with satisfactory recovery and precision. Taken together, this study provides an effective detection technology for the monitoring and risk control of BA in tremella and auricularia auricular.
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http://dx.doi.org/10.3724/SP.J.1123.2021.06013DOI Listing
December 2021

Clinical Features and Temporal Lung Radiographic Changes in 25 Patients Recovering from COVID-19 Pneumonia: A Retrospective Case-Control Study.

Med Sci Monit 2021 Nov 14;27:e933381. Epub 2021 Nov 14.

Department of Pulmonary and Critical Care Medicine, Xiangtan Central Hospital, Xiangtan, Hunan, China (mainland).

BACKGROUND Little is known of the changes in lung radiographic characteristics over time in patients recovering from COVID-19. This study analyzed the clinical features and temporal lung radiographic changes in patients with moderate and severe COVID-19 pneumonia who did not require invasive mechanical ventilation during the acute and convalescent periods. MATERIAL AND METHODS The data of 25 patients with COVID-19 pneumonia from January 29, 2020, to November 24, 2020, who did not require invasive mechanical ventilation and who were followed up were retrospectively collected. The 25 patients were divided into severe and moderate groups. Clinical characteristics and computed tomography (CT) manifestations were compared. A total of 121 consecutive thin-slice CT scans were collected at 4 weeks, 2 months, and 5 months after admission to evaluate lung abnormalities in the patients. The CT score was used to assess disease severity. RESULTS The severe group had a lower rate of nucleic acid conversion within 10 days of admission and higher D-dimer, creatine kinase, and lactate dehydrogenase values. In the severe group, hospital stay was longer and hospitalization costs were higher. The average CT score of the severe group peaked in the second week, while the moderate group peaked in the first week and then decreased over time. There were no statistically significant differences in the average CT score between the 2 groups at the 5-month follow-up. CONCLUSIONS The pulmonary lesions of patients recovering from COVID-19 and who do not require invasive mechanical ventilation were gradually absorbed and resolved over time.
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http://dx.doi.org/10.12659/MSM.933381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600820PMC
November 2021

UPLC-Q-TOF-MS based metabolomics study of hawthorn leaves in different geographical regions.

Anal Methods 2021 11 25;13(45):5458-5466. Epub 2021 Nov 25.

Hebei Province Key Laboratory of Research and Development for Chinese Medicine, Chengde Medical University, Chengde 067000, Hebei, China.

The quality evaluation of hawthorn leaves in different geographical regions derived from the dried leaves of Bge. Var. Major N.E.Br. or Bge., a common blood-activating and stasis-eliminating traditional Chinese medicine, has hardly been reported. In this study, the chemical comparison of 40 batches of hawthorn leaf samples collected from Hebei, Liaoning, Shandong and Shanxi Provinces was performed using an ultra-high performance liquid chromatography with electrospray ionization-tandem mass spectrometry-based metabolic profile and pattern recognition analysis approach. A total of 233 compounds were determined. Among them, 40 compounds were selected as potential metabolites responsible for the differential clustering, and the differential metabolite-based evaluation model was applied to well distinguish the origin of seven batches of hawthorn leaves sold on the market. Further analysis of the KEGG pathway showed that five core metabolites containing flavonoids and lignins were mainly involved in flavonoid biosynthesis, flavone and flavonol biosynthesis, and stilbenoid, diarylheptanoid and gingerol biosynthesis. Taking the content of flavonoids, core markers, as the evaluation basis, it was found that the quality of hawthorn leaves in Hebei and Liaoning was better. The study provides a reference for the rational utilization of hawthorn leaves, and highlights that the metabolomics-driven analysis method is more suitable for the quality evaluation of traditional Chinese medicine.
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http://dx.doi.org/10.1039/d1ay01150bDOI Listing
November 2021
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