Publications by authors named "Hong Wang"

5,591 Publications

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Danshensu prevents thrombosis by inhibiting platelet activation via SIRT1/ROS/mtDNA pathways without increasing bleeding risk.

Phytomedicine 2022 Jun 12;104:154271. Epub 2022 Jun 12.

State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 301617 Tianjin, China; Key Laboratory of Pharmacology of Traditional Chinese Medical Formula, Ministry of Education, Tianjin University of Traditional Chinese Medicine, 301617 Tianjin, China; Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, 301617 Tianjin, China; Tianjin State Key Laboratory of Modern Chinese Medicine, 301617 Tianjin, China; School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, 301617 Tianjin, China. Electronic address:

Background: Coronary thrombosis and its correlated disorders are main healthcare problems globally. The therapeutic effects of current treatments involving antiplatelet drugs are not fully satisfactory. Danshensu (DSS) is an important monomer obtained from Salvia miltiorrhiza roots that have been widely employed for vascular diseases in medicinal practices. Nonetheless, the underlying mechanisms of DSS are not fully unraveled.

Purpose: The objective of this study was to penetrate the antithrombotic and antiplatelet mechanisms of DSS.

Methods: Network pharmacology assay was used to forecast the cellular mechanisms of DSS for treating thrombosis. The work focused the impacts of DSS on platelet activation by analyzing aggregation and adhesion in vitro. Flow cytometry, western blotting, CM-H2DCFDA staining and mitochondrial function assays were performed to reveal the molecular mechanisms. The model of common carotid artery thrombus induced by ferric chloride was established. The wet weight of thrombus was measured, and the thrombosis was observed by hematoxylin and eosin (H&E) staining, in order to support the inhibitory effect of DSS on thrombosis.

Results: Data mining found the antithrombotic effect of DSS is related to platelet activation and the core target is silent information regulator 1 (SIRT1). We confirmed that DSS dose-dependently inhibited platelet activation in vitro. DSS was further demonstrated to induce the expression of SIRT1 and decreased reactive oxygen species (ROS) burden and thereby prevented mitochondrial dysfunction. Mitochondrial function tests further indicated that DSS prevented mitochondrial DNA (mtDNA) release, which induced activation of platelet in a dendritic cell specific intercellular-adhesion-molecule-3 grabbing non-integrin (DC-SIGN)-dependent manner. In carotid artery injury model induced by ferric chloride, DSS inhibited the development of carotid arterial thrombosis. More encouragingly, in tail bleeding time assay, DSS did not augment bleeding risk.

Conclusion: These findings indicated that DSS effectively inhibited platelet activation by depressing the collection of ROS and the release of platelet mtDNA without arousing hemorrhage risk. DSS might represent a promising candidate drug for thrombosis and cardiovascular disease therapeutics.
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http://dx.doi.org/10.1016/j.phymed.2022.154271DOI Listing
June 2022

Electrospinning of PLA Nanofibers: Recent Advances and Its Potential Application for Food Packaging.

J Agric Food Chem 2022 Jul 1. Epub 2022 Jul 1.

College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.

Poly(lactic acid), also abbreviated as PLA, is a promising biopolymer for food packaging owing to its environmental-friendly characteristic and desirable physical properties. Electrospinning technology makes the production of PLA-based nanomaterials available with expected structures and enhanced barrier, mechanical, and thermal properties; especially, the facile process produces a high encapsulation efficiency and controlled release of bioactive agents for the purpose of extending the shelf life and promoting the quality of foodstuffs. In this study, different types of electrospinning techniques used for the preparation of PLA-based nanofibers are summarized, and the enhanced properties of which are also described. Moreover, its application in active and intelligent packaging materials by introducing different components into nanofibers is highlighted. In all, the review establishes the promising prospects of PLA-based nanocomposites for food packaging application.
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http://dx.doi.org/10.1021/acs.jafc.2c02611DOI Listing
July 2022

: A Prolific Source of Bioactive Secondary Metabolites with Therapeutic Potential.

J Med Chem 2022 Jun 29. Epub 2022 Jun 29.

School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.

, one of the most important actinomycetes genera, is well-known as the treasure trove of bioactive secondary metabolites (SMs). Herein, together with an in-depth genomic analysis of the reported strains, all SMs from this genus are comprehensively summarized, containing structural features, bioactive properties, and mode of actions as well as their biosynthetic and chemical synthesis pathways. The perspective enables a detailed view of -derived SMs, which will enrich the chemical diversity of natural products and inspire new drug discovery in the pharmaceutical industry.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00626DOI Listing
June 2022

Comparison of Reproductive Function Between Normal and Hyperandrogenemia Conditions in Female Mice With Deletion of Hepatic Androgen Receptor.

Front Endocrinol (Lausanne) 2022 9;13:868572. Epub 2022 Jun 9.

Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, United States.

Obesity, altered glucose homeostasis, hyperinsulinism, and reproductive dysfunction develops in female humans and mammals with hyperandrogenism. We previously reported that low dose dihydrotestosterone (DHT) administration results in metabolic and reproductive dysfunction in the absence of obesity in female mice, and conditional knock-out of the androgen receptor (Ar) in the liver (LivARKO) protects female mice from DHT-induced glucose intolerance and hyperinsulinemia. Since altered metabolic function will regulate reproduction, and liver plays a pivotal role in the reversible regulation of reproductive function, we sought to determine the reproductive phenotype of LivARKO mice under normal and hyperandrogenemic conditions. Using Cre/Lox technology, we deleted the in the liver, and we observed LivARKO female mice have normal puberty timing, cyclicity and reproductive function. After DHT treatment, like control mice, LivARKO experience altered estrous cycling, reduced numbers of corpus lutea, and infertility. Liver Ar is not involved in hyperandrogenemia-induced reproductive dysfunction. The reproductive dysfunction in the DHT-treated LivARKO lean females with normal glucose homeostasis indicates that androgen-induced reproductive dysfunction is independent from metabolic dysfunction.
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http://dx.doi.org/10.3389/fendo.2022.868572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218244PMC
June 2022

Aberrant nucleosome organization in mouse SCNT embryos revealed by ULI-MNase-seq.

Stem Cell Reports 2022 Jun 9. Epub 2022 Jun 9.

Clinical and Translation Research Center of Shanghai First Maternity & Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China. Electronic address:

Somatic cell nuclear transfer (SCNT) can reprogram terminally differentiated somatic cells into totipotent embryos, but with multiple defects. The nucleosome positioning, as an important epigenetic regulator for gene expression, is largely unexplored during SCNT embryonic development. Here, we mapped genome-wide nucleosome profiles in mouse SCNT embryos using ultra-low-input MNase-seq (ULI-MNase-seq). We found that the nucleosome-depleted regions (NDRs) around promoters underwent dramatic reestablishment, which is consistent with the cell cycle. Dynamics of nucleosome position in SCNT embryos were delayed compared to fertilized embryos. Subsequently, we found that the aberrant gene expression levels in inner cell mass (ICM) were positively correlated with promoter NDRs in donor cells, which indicated that the memory of nucleosome occupancy in donor cells was a potential barrier for SCNT-mediated reprogramming. We further confirmed that the histone acetylation level of donor cells was associated with the memory of promoter NDRs. Our study provides insight into nucleosome reconfiguration during SCNT preimplantation embryonic development.
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http://dx.doi.org/10.1016/j.stemcr.2022.05.020DOI Listing
June 2022

Controllable synthesis of gold nanoparticle dimers site-selective growth.

Chem Commun (Camb) 2022 Jun 24. Epub 2022 Jun 24.

Department of Environmental Science and Engineering, University of Science and Technology of China, 230026 Hefei, China.

Random aggregation of nanoparticles follows step-growth kinetics, and thus the synthesis of large nanoparticle dimers is still a challenge. Here, we report a site-selective growth route to producing gold nanoparticle dimers in a high yield of up to 71%. The polymer contraction exposed two tips of gold nanorods, which provided active sites for further growth, setting the ability for a high yield of large dimers. Methods to fine tune the reaction rate allowed us to control the spacing between nanoparticles, giving a good SERS performance. Our method was shown to make up for the shortcomings of the existing synthetic method, and to achieve the synthesis of large dimers in solution.
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http://dx.doi.org/10.1039/d2cc00801gDOI Listing
June 2022

In Situ Atomic-Scale Observation of Electrochemical (De)potassiation in Te Nanowires.

Small 2022 Jun 24:e2200844. Epub 2022 Jun 24.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, 430070, P. R. China.

Potassium-ion batteries (PIBs) have great potential in energy storage due to their high abundance and low cost of potassium resources. Tellurium (Te) is a promising PIB cathode due to its high volumetric capacity and good electronic conductivity. However, the electrochemical (de)potassiation mechanism of Te remains elusive due to the lack of an effective method of directly observing the dynamic reaction at atomic resolution. Here, the phase transformations of single crystal Te on (de)potassiation are clearly revealed by in situ high-resolution transmission electron microscopy and electron diffraction. Te undergoes a consecutive phase transformation during potassiation: from Te to K Te in the initial potassiation, and then part of the K Te to K Te on further potassiation. The reaction has extremely high reversibility in the following depotassiation. By atomic-scale observation, an anisotropic reaction mechanism where K intercalates into Te crystalline lattice preferentially through the (001) plane (having a large d-spacing) is established during potassiation. While in the depotassiation process, K ions extract from the polycrystalline K Te along the same diffusion path to form single crystal Te, indicating the potassium storage is highly reversible. The strong orientation-dependent (de)potassiation mechanism revealed by this work provides implications for the future design of nanostructured cathodes for high-performance PIBs.
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http://dx.doi.org/10.1002/smll.202200844DOI Listing
June 2022

Flexible Humidity Sensors Based on Multidimensional Titanium Dioxide/Cellulose Nanocrystals Composite Film.

Nanomaterials (Basel) 2022 Jun 8;12(12). Epub 2022 Jun 8.

Key Laboratory of Recycling and Eco-Treatment of Waste Biomass of Zhejiang Province, Zhejiang University of Science and Technology, Hangzhou 310023, China.

A humidity sensor is a crucial device in daily life; therefore, in the present study, a novel humidity sensor was designed to increase its specific surface area to improve its humid sensing capacity and conductivity. Titanium dioxide nanoparticles (TiNP) consisting of zero-dimensional nanospheres and one-dimensional nanotubes were prepared by anodic oxidation. Rod-shaped cellulose nanocrystals (CNCs) with average length and diameter of 60 nm and 800 nm, respectively, were obtained by enzymatic hydrolysis and high pressure homogenization. TiNP/CNC composite films exhibited superior hydrophilicity and large specific surface areas based on Fourier transform infrared spectroscopy and nitrogen adsorption-desorption results. The humidity sensing characteristics of sensors based on TiNP/CNC flexible composite films with varying contents of TiNP were investigated under a relative humidity range of 11-97%. The 6% TiNP/CNC-based humidity sensor exhibited high humidity response, rapid response/recovery speed, and high stability. Furthermore, the humidity sensing mechanism of TiNP/CNC composite films was analyzed based on the density functional theory. TiNP/CNC-based humidity sensors could be applied in flexible and wearable electronics.
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http://dx.doi.org/10.3390/nano12121970DOI Listing
June 2022

Investigation of the Uptake and Transport of Two Novel Camptothecin Derivatives in Caco-2 Cell Monolayers.

Molecules 2022 Jun 7;27(12). Epub 2022 Jun 7.

College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China.

Irinotecan and Topotecan are two Camptothecin derivatives (CPTs) whose resistance is associated with the high expression of breast cancer resistance protein (BCRP) and P-glycoprotein (P-gp). To reverse this resistance, two novel CPTs, FL77-28 (7-(3-Fluoro-4-methylphenyl)-10,11-methylenedioxy-20(S)-CPT) and FL77-29 (7-(4-Fluoro-3-methylphenyl)-10,11-methylenedioxy-20(S)-CPT), were synthesized by our group. In this study, the anti-tumor activities of FL77-28, FL77-29, and their parent, FL118 (10,11-methylenedioxy-20(S)-CPT), were evaluated and the results showed that FL77-28 and FL77-29 had stronger anti-tumor activities than FL118. The transport and uptake of FL118, FL77-28, and FL77-29 were investigated in Caco-2 cells for the preliminary prediction of intestinal absorption. The apparent permeability coefficient from apical to basolateral (P) values of FL77-28 and FL77-29 were (2.32 ± 0.04) × 10 cm/s and (2.48 ± 0.18) × 10 cm/s, respectively, suggesting that the compounds had moderate absorption. Since the transport property of FL77-28 was passive diffusion and the efflux ratio (ER) was less than 2, two chemical inhibitors were added to further confirm the involvement of efflux proteins. The results showed that FL77-28 was not a substrate of P-gp or BCRP, but FL77-29 was mediated by P-gp. In conclusion, FL77-28 might be a promising candidate to overcome drug resistance induced by multiple efflux proteins.
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http://dx.doi.org/10.3390/molecules27123669DOI Listing
June 2022

Vaccines Using Sporulation Proteins Reduce Necrotic Enteritis in Chickens.

Microorganisms 2022 May 27;10(6). Epub 2022 May 27.

Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.

is the prevalent enteric pathogen in humans and animals including chickens, and it remains largely elusive on the mechanism of -induced enteritis because of limited animal models available. In this study, we investigated the role of sporulation proteins as vaccine candidates in chickens to reduce necrotic enteritis (NE). soluble proteins of vegetative cells (CP-super1 and CP-super2) and spores (CP-spor-super1 and CP-spor-super2) were prepared, and cell and chicken experiments were conducted. We found that deoxycholic acid reduced invasion and sporulation using the and co-infection necrotic enteritis (NE) model. enterotoxin (CPE) was detected in the CP-spor-super1&2. CP-spor-super1 or 2 induced cell death in mouse epithelial CMT-93 and macrophage Raw 264.7 cells. CP-spor-super1 or 2 also induced inflammatory gene expression and necrosis in the Raw cells. Birds immunized with CP-spor-super1 or 2 were resistant to -induced severe clinical NE on histopathology and body weight gain loss. CP-spor-super1 vaccine reduced NE-induced proinflammatory gene expression as well as luminal colonization and tissue invasion in the small intestine. Together, this study showed that CP-spor-super vaccines reduced NE histopathology and productivity loss.
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http://dx.doi.org/10.3390/microorganisms10061110DOI Listing
May 2022

Reduction in RF Loss Based on AlGaN Back-Barrier Structure Changes.

Micromachines (Basel) 2022 May 26;13(6). Epub 2022 May 26.

The Engineering Research Center for Optoelectronics of Guangdong Province, School of Physics and Optoelectronics, South China University of Technology, Guangzhou 510640, China.

We designed a high electron mobility transistor (HEMT) epitaxial structure based on an AlGaN/GaN heterojunction, utilizing Silvaco TCAD, and selected AlGaN with an aluminum composition of 0.1 as the back-barrier of the AlGaN/GaN heterojunction. We enhanced the confinement of the two-dimensional electron gas (2DEG) by optimizing the structural parameters of the back barrier, so that the leakage current of the buffer layer is reduced. Through these optimization methods, a lower drain leakage current and a good radio frequency performance were obtained. The device has a cut-off frequency of 48.9 GHz, a maximum oscillation frequency of 73.20 GHz, and a radio frequency loss of 0.239 dB/mm (at 6 GHz). This work provides a basis for the preparation of radio frequency devices with excellent frequency characteristics and low RF loss.
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http://dx.doi.org/10.3390/mi13060830DOI Listing
May 2022

Associations between Polyfluoroalkyl Substances Exposure and Breast Cancer: A Meta-Analysis.

Toxics 2022 Jun 11;10(6). Epub 2022 Jun 11.

School of Public Health, Wuhan University, Wuhan 430071, China.

Polyfluoroalkyl substances (PFASs) are persistent pollutants that may cause breast cancer. However, associations between exposure to PFASs and the risk of breast cancer are controversial. We retrieved studies on the association between PFASs-perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorohexane sulfonic acid (PFHxS), and perfluorooctane sulfonic acid (PFOS)-and breast cancer risk in women from PubMed, Embase, and the Web of Science. The pooled odds ratios (ORs) or relative risks (RRs) and their 95% confidence intervals (CIs) were extracted or calculated from provided data. Moreover, subgroup and metaregression analyses were performed to distinguish the potential sources of heterogeneity between studies. Lastly, eight original studies were included in the meta-analysis. PFOA and PFHxS were positively correlated with breast cancer risk, and the pooled ORs (and 95% CIs) were 1.32 (1.19 and 1.46) and 1.79 (1.51 and 2.11), respectively. PFNA was negatively correlated with breast cancer risk and the pooled OR (and 95% CIs) was 0.76 (0.6 and 0.96), and PFOS was shown to have no correlation with breast cancer risk and the pooled OR (and 95% CIs) was 1.01 (0.87 and 1.17). All results were merged in a random-effects model with significant heterogeneities ( > 90%, < 0.001). The results demonstrated that PFASs might be potential risk factors for breast cancer, and the compounds in low exposure levels could have a more harmful impact on human health.
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http://dx.doi.org/10.3390/toxics10060318DOI Listing
June 2022

Genome Features and AntiSMASH Analysis of an Endophytic Strain sp. R1.

Metabolites 2022 Jun 4;12(6). Epub 2022 Jun 4.

School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.

Endophytic fungi are one of the most prolific sources of functional biomolecules with therapeutic potential. Besides playing an important role in serious plant diseases, strains possess the powerful capability to produce a diverse array of bioactive secondary metabolites (SMs). In order to in-depth mine gene clusters for SM biosynthesis of the genus , an endophytic strain sp. R1 isolated from Makino was extensively investigated by whole-genome sequencing and in-depth bioinformatic analysis, as well as antiSMASH annotation. The results displayed that strain R1 harbors a total of 51.8 Mb genome, which consists of 542 contigs with an N50 scaffold length of 3.21 Mb and 50.4% GC content. Meanwhile, 19,333 functional protein-coding genes, 338 tRNA and 111 rRNA were comprehensively predicted and highly annotated using various BLAST databases including non-redundant (Nr) protein sequence, nucleotide (Nt) sequence, Swiss-Prot, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG), as well as Pathogen Host Interactions (PHI) and Carbohydrate-Active enzymes (CAZy) databases. Antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) results showed that strain R1 has 37 SM biosynthetic gene clusters (BGCs), including 17 nonribosomal peptide synthetases (NRPSs), 13 polyketide synthetases (PKSs), 3 terpene synthases (Ts), 3 hybrid NRPS + PKS and 1 hybrid indole + NRPS. These findings improve our knowledge of the molecular biology of the genus and would promote the discovery of new bioactive SMs from strain R1 using gene mining strategies including gene knockout and heteroexpression.
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http://dx.doi.org/10.3390/metabo12060521DOI Listing
June 2022

Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain L14 Unveiling Its Vast Potential of Secondary Metabolites Biosynthesis.

J Fungi (Basel) 2022 May 31;8(6). Epub 2022 May 31.

School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.

is one of the most important sources of secondary metabolites (SMs), with a wide array of pharmacological effects, including anti-inflammatory, antitumor, immunomodulatory and antioxidant effects. However, the biosynthetic analysis of these bioactive components has been rarely reported owing to the lack of high-quality genome sequences and comprehensive analysis. In this study, the whole genome of one marine-sponge-derived strain L14 was sequenced and assembled as well as in-depth bioinformatic analysis. The results indicated that the sequence assembly of strain L14 generated one high-quality genome with a total size of 36.1 Mb, a G + C content of 45.3% and an N50 scaffold of 4.2 Mb. Gene annotation was extensively deployed using various BLAST databases, including non-redudant (Nr) protein sequence, nucleotide (Nt) sequence, Swiss-Prot, Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) as well as Pathogen Host Interactions (PHI) and Carbohydrate-active enzymes (CAZy) databases. AntiSMASH analysis revealed that this marine strain harbors a total of 69 SMs biosynthesis gene clusters (BGCs), including 17 PKSs, 18 NRPSs, 21 NRPS-likes, 9 terpenes, 2 indoles, 1 betalactone and 1 siderophore, suggesting its biosynthetic potential to produce a wide variety of SMs. These findings will assist in future investigations on the genetic basis of strain L14 and provide insights into its new bioactive SMs for new drug discovery.
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http://dx.doi.org/10.3390/jof8060591DOI Listing
May 2022

Volume-controlled ventilation versus pressure-controlled ventilation during spine surgery in the prone position: A meta-analysis.

Ann Med Surg (Lond) 2022 Jun 25;78:103878. Epub 2022 May 25.

Department of Spine Surgery, Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian, 116033, Liaoning, China.

Background: Many studies have investigated a comparison of the potency and safety of PCV versus VCV modes in spinal surgery in prone position. However, controversy about the maximal benefits of which ventilation modes remains. The main purpose of this meta-analysis was to investigate which one is the optimal ventilation for surgery patients undergoing spine surgery in prone position between the two ventilation modes as PCV and VCV.

Methods: We conducted a comprehensive search of PubMed, Embase, Web of Science, the Cochrane Library, and Google Scholar for potentially eligible articles. The continuous outcomes were analyzed using the mean difference and the associated 95% confidence interval. Meta-analysis was performed using Review Manager 5.4 software.

Results: Our meta-analysis included 8 RCTs involving a total of 454 patients between 2012 and 2020. The results demonstrated that IOB, Ppeak and CVP for VCV are significantly superior to PCV in spinal surgery in prone position. And PCV had higher Cdyn and PaO/FiO than VCV. But there was no significant difference between PCV and VCV in terms of POB, Hb, HCT, HR and MAP.

Conclusions: The PCV mode displayed a more satisfying effect than VCV mode. Compared to VCV mode in same preset of tidal volume, the patients with PCV mode in prone position demonstrated less IOB, lower Ppeak and CVP, and higher PaO/FiO in spinal surgery. However, there is no obvious difference between PCV and VCV in terms of hemodynamics variables (HR and MAP).
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http://dx.doi.org/10.1016/j.amsu.2022.103878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207057PMC
June 2022

Peptidylarginine Deiminase 2 Autoantibodies Are Linked to Less Severe Disease in Multiple Sclerosis and Post-treatment Lyme Disease.

Front Neurol 2022 6;13:874211. Epub 2022 Jun 6.

Division of Rheumatology, The Johns Hopkins University School of Medicine, Baltimore, MD, United States.

Background: Peptidylarginine deiminase 2 (PAD2) mediates the post-translational conversion of arginine residues in proteins to citrullines and is highly expressed in the central nervous system (CNS). Dysregulated PAD2 activity has been implicated in the pathogenesis of several neurologic diseases, including multiple sclerosis (MS). In this study, we sought to define the cellular and regional expression of the gene encoding for PAD2 (i.e. ) in the human CNS using publicly available datasets and evaluate whether anti-PAD2 antibodies were present in patients with various neurologic diseases.

Methods: A total of 491 study participants were included in this study: 91 people with MS, 32 people with neuromyelitis optica (NMO), 281 people with post-treatment Lyme disease (PTLD), and 87 healthy controls. To measure expression in the CNS from healthy individuals, publicly available tissue and single cell RNA sequencing data was analyzed. Anti-PAD2 antibodies were measured in the serum of study participants using anti-PAD2 ELISA. Clinical and demographic variables were compared according to anti-PAD2 antibody positivity for the MS and PTLD groups and correlations between anti-PAD2 levels and disease severity were examined.

Results: expression was highest in oligodendrocytes (mean ± SD; 6.4 ± 2.2), followed closely by astrocytes (5.5 ± 2.6), microglia/macrophages (4.5 ± 3.5), and oligodendrocyte precursor cells (3.2 ± 3.3). There was an increased proportion of anti-PAD2 positivity in the MS (19.8%; = 0.007) and PTLD groups (13.9%; = 0.057) relative to the healthy controls (5.7%), and these antibodies were not detected in NMO patients. There was a modest inverse correlation between anti-PAD2 levels and disease severity in people with MS (τ = -0.145, = 0.02), with levels being the highest in those with relapsing-remitting disease. Similarly, there was a modest inverse correlation between anti-PAD2 levels and neurocognitive score (τ = -0.10, = 0.027) in people with PTLD, with difficulty focusing, memory changes, fatigue, and difficulty finding words contributing most strongly to the effect.

Conclusion: expression was observed in diverse regions and cells of the CNS, and anti-PAD2 autoantibodies were associated with less severe symptoms in subsets of patients with MS and PTLD. These data suggest that anti-PAD2 antibodies may attenuate inflammation in diseases of different etiologies, which are united by high expression in the target tissue.
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http://dx.doi.org/10.3389/fneur.2022.874211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207393PMC
June 2022

Reduction of SLC7A11 and GPX4 Contributing to Ferroptosis in Sperm from Asthenozoospermia Individuals.

Reprod Sci 2022 Jun 21. Epub 2022 Jun 21.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Ferroptosis is a newly defined form of regulated cell death, which is involved in various pathophysiological conditions. However, the role of ferroptosis in male infertility remains unclear. In this study, 42 asthenozoospermic and 45 normozoospermic individuals participated. To investigate the ferroptosis level in the two groups, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and iron were measured, and mitochondrial membrane potential (MMP) was detected as an indicator of mitochondrial injuries. Compared with the normozoospermic group, ROS (p < 0.05), MDA (p < 0.001), and iron (p < 0.001) of the asthenozoospermic group were significantly increased. However, the asthenozoospermia group had a decreased MMP level (p < 0.05). In addition, the expression levels of GSH-dependent peroxidase 4 (GPX4) (p < 0.001) and solute carrier family 7 member 11 (SLC7A11) (p < 0.05) were also reduced in asthenozoospermic individuals. In asthenozoospermic samples, a significantly high positive correlation was observed between GPX4 mRNA levels and progressive motility (r = 0.397, p = 0.009) and total motility (r = 0.389, p = 0.011), while a negative correlation was observed between GPX4 and iron concentration (r =  - 0.276, p = 0.077). The function of ferroptosis in asthenozoospermic males has never been studied before. In our study, we concluded that GPX4 and SLC7A11 expression levels in asthenozoospermia patients were related to increased ferroptosis and impaired sperm function, revealing novel molecular insights into the complex systems involved in male infertility.
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http://dx.doi.org/10.1007/s43032-022-01004-yDOI Listing
June 2022

Concurrently Achieving High Discharged Energy Density and Efficiency in Composites by Introducing Ultralow Loadings of Core-Shell Structured [email protected] Nanoboxes.

ACS Appl Mater Interfaces 2022 Jun 21;14(25):29292-29301. Epub 2022 Jun 21.

Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

Polymer dielectrics have drawn tremendous attention worldwide due to their huge potential for pulsed power capacitors. Recent studies have demonstrated that linear/nonlinear layered composites, which can effectively balance energy density and efficiency, have huge potential for capacitive energy storage applications. However, further enhanced energy densities are strongly desired to meet the everincreasing demand for the miniaturization of electronic devices. Herein, a novel class of core-shell structured [email protected] dioxide nanoboxes is successfully synthesized and introduced into poly(vinylidene fluoride-hexafluoropropylene)-poly(ether imide) double-layer films. It is exciting to find that the introduction of merely 0.5 wt % nanoboxes results in a substantially enhanced energy density of 19.39 J/cm, which is over 2.6 times that of the film without nanoboxes (7.44 J/cm). Meanwhile, a high breakdown strength of 655 kV/mm and a high efficiency of 83% are achieved. Furthermore, the nanocomposites also show excellent power densities and cycling stabilities. These composites with excellent comprehensive energy storage performances have huge potential for advanced pulsed power capacitors.
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http://dx.doi.org/10.1021/acsami.2c07229DOI Listing
June 2022

Phylogenomics and evolutionary history of Oreocnide (Urticaceae) shed light on recent geological and climatic events in SE Asia.

Mol Phylogenet Evol 2022 Jun 17:107555. Epub 2022 Jun 17.

Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China. Electronic address:

Climate change and geological events have long been known to shape biodiversity, implying that these can likewise be viewed from a biological perspective. To study whether plants can shed light on this, and how they responded to climate change there, we examined Oreocnide, a genus widely distributed in SE Asia. Based on broad geographic sampling with genomic data, we employed an integrative approach of phylogenomics, molecular dating, historical biogeography, and ecological analyses. We found that Oreocnide originated in mainland East Asia and began to diversify ∼6.06 Ma, probably in response to a distinct geographic and climatic transition in East Asia at around that time, implying that the last important geological change in mainland SE Asia might be 1 Ma older than previously suggested. Around four immigration events to the islands of Malesia followed, indicating that immigration from the mainland could be an underestimated factor in the assembly of biotic communities in the region. Two detected increases of diversification rate occurred 3.13 and 1.19 Ma, which strongly implicated climatic rather than geological changes as likely drivers of diversification, with candidates being the Pliocene intensification of the East Asian monsoons, and Pleistocene climate and sea level fluctuations. Distribution modelling indicated that Pleistocene sea level and climate fluctuations were inferred to enable inter-island dispersal followed by allopatric separation, underpinning radiation in the genus. Overall, our study, based on multiple lines of evidence, linked plant diversification to the most recent climatic and geological events in SE Asia. We highlight the importance of immigration in the assembly and diversification of the SE Asian flora, and underscore the utility of plant clades, as independent lines of evidence, for reconstructing recent climatic and geological events in the SE Asian region.
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http://dx.doi.org/10.1016/j.ympev.2022.107555DOI Listing
June 2022

Tandem Reactions for the Synthesis of High-Density Polycyclic Biofuels with a Double/Triple Hexane Ring.

ACS Omega 2022 Jun 30;7(23):19158-19165. Epub 2022 May 30.

State Key Laboratory of Multiphase Flow in Power Engineering & Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710054, China.

Synthesizing high-density fuels from non-food biomass is of great interest in the field of biomass conversion because they can increase the loading capability and travel distance of vehicles and aircraft as compared to conventional low-density biofuels (<0.78 g/cm). In this work, we reported a new and facile strategy for the synthesis of high-density biofuels with polycyclic structures from lignocellulose-derived 5,5-dimethyl-1,3-cyclohexanedione and different aldehyde derivatives in two steps under mild reaction conditions, including a developed tandem reaction and a hydrodeoxygenation reaction. Theoretical approaches were used to estimate the fuel properties, which indicate that the obtained biofuels have a high density of 0.78-0.88 g/cm and high net heat of combustion (NHOC) values of 44.0-46.0 MJ/kg. A representative biofuel was measured to have a high NHOC of 43.4 MJ/kg, which matched well with the calculated NHOC value of 44.4 MJ/kg, indicating the high accuracy of the theoretical approaches. This work is expected to provide a green strategy for the synthesis of polycyclic high-density biofuels with platform chemicals.
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http://dx.doi.org/10.1021/acsomega.1c07241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202244PMC
June 2022

Effects of Incontro, Alleanza, Responsabilita, Autonomia Intervention Model Combined with Orem Self-Care Model and the Use of Smart Wearable Devices on Perceived Stress and Self-Efficacy in Patients after Total Hip Arthroplasty.

Comput Intell Neurosci 2022 9;2022:5780084. Epub 2022 Jun 9.

Medical College of Yangzhou University, Yangzhou, Jiangsu Province 225009, China.

Objective: To explore the effects of Incontro, Alleanza, Responsabilita, Autonomia (IARA) combined with Orem self-care model and the use of smart wearable devices on perceived stress and self-efficacy in patients after total hip arthroplasty (THA).

Methods: A total of 60 patients after THA in our hospital were enrolled. Patients were randomly divided into control group (IARA intervention model combined with Orem self-care model) and study group (intelligent wearable device combined conference-IARA and Orem self-care model). Harris hip function score, Western Ontario and McMaster Universities Arthritis Index (WOMAC) score, functional independence measure (FIM) score, social support level, perceived stress, and self-efficacy were compared between the two groups.

Results: Harris hip function score, WOMAC score, FIM score, and the level of social support of the study group were higher compared with the control group after operation ( < 0.05). Additionally, the perceptual pressure in the study group was lower compared with the control group after intervention ( < 0.05). The self-efficacy of the two groups was compared, and the self-efficacy of the study group was higher than that of the control group at 4, 6, 8, and 12 weeks after the intervention, and the difference was statistically significant ( < 0.05).

Conclusion: Patients after THA utilize an intelligent wearable device combined with IARA model and Orem self-care model, which can effectively reduce awareness pressure, improve self-efficacy, and facilitate the improvement of the hip fracture.
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http://dx.doi.org/10.1155/2022/5780084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203192PMC
June 2022

Autophagy Induced by Muscarinic Acetylcholine Receptor 1 Mediates Migration and Invasion Targeting Atg5 via AMPK/mTOR Pathway in Prostate Cancer.

J Oncol 2022 9;2022:6523195. Epub 2022 Jun 9.

Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China.

Increasing numbers of researchers discovered the expression of muscarinic acetylcholine receptor 1 in human cancers, while its function in human prostate cancer is still unclear. Our present study focused on CHRM1 to clarify its role in mediating autophagy in prostate cancer. We used immunohistochemistry, western blotting, and immunofluorescence experiments to observe the expression of muscarinic acetylcholine receptor 1 both in nude mice with subcutaneous tumors and in prostate cancer cells. The autophagy was observed through transmission electron microscopy, western blotting, quantitative real-time PCR, and immunofluorescence. After that, we used lentivirus to establish CHRM1 and Atg5 knockdown models. Then, the migration and invasion abilities after knocking down muscarinic acetylcholine receptor 1 and Atg5 were detected by transwell assays. In addition, the AMPK/mTOR pathway-related targets were detected by western blotting. We found that muscarinic acetylcholine receptor 1 was abundantly expressed both in vitro and in vivo in prostate cancer. The overexpression of muscarinic acetylcholine receptor 1 positively regulated migration and invasion in tumor cells as well as the activation of autophagy. Muscarinic acetylcholine receptor 1 was highly correlated with Atg5 and activated the AMPK/mTOR signaling pathway. Downregulation of Atg5 inhibited cell autophagy in prostate cancer cells and the migration and invasion of prostate cancer cells. Meanwhile, abnormal expressions of AMPK/mTOR pathway-related proteins were found. In conclusion, the present findings indicated that muscarinic acetylcholine receptor 1 is highly expressed in prostate cancer cells and promotes cell invasion and migration of prostate cancer. Autophagy is activated in prostate cancer cells and the activation of muscarinic acetylcholine receptor 1 positively regulates autophagy in prostate cancer cells. Moreover, muscarinic acetylcholine receptor 1 induces autophagy-mediated cell migration and invasion by targeting Atg5 in prostate cancer cells via AMPK/mTOR pathway, which uncovered that regulating muscarinic acetylcholine receptor 1, identified in this study, can be a promising solution for treating prostate cancer.
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http://dx.doi.org/10.1155/2022/6523195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203210PMC
June 2022

[Systematic review and Meta-analysis of efficacy and safety of Shuxuetong Injection in treatment of diabetic peripheral neuropathy].

Zhongguo Zhong Yao Za Zhi 2022 Jun;47(11):3088-3094

Beijing Hospital of Traditional Chinese Medicine, Capital Medical University Beijing 100010, China.

The present study evaluated the efficacy and safety of Shuxuetong Injection in the treatment of diabetic peripheral neuropathy(DPN). PubMed, Cochrane Library, EMbase, CNKI, VIP, SinoMed, and Wanfang were searched for randomized controlled trials(RCTs) of Shuxuetong Injection in the treatment of DPN from database inception to July 18, 2021. Literature screening was carried out according to the inclusion and exclusion criteria, and the required information was extracted. Cochrane risk-of-bias tool was used to evaluate the quality of the included trials, and RevMan 5.3 was used for Meta-analysis. Finally, six articles were included, involving 507 patients. The overall quality of included RCTs was not high. As revealed by Meta-analysis results, in terms of DPN score, one trial showed a significant difference in the experimental group before and after treatment, and the symptom of the experimental group was significantly improved compared with that in the control group after treatment. After treatment, a significant difference in DPN scores of the experimental group and the control group was observed(P<0.05), and the symptom improvement of the experimental group was significantly better than that of the control group. In terms of sensory nerve conduction velocity(SCV), the experimental group was superior to the control group in improving conduction velocities(MD_(median SCV)=4.45, 95%CI[2.79, 6.10], P<0.000 01; MD_(peroneal SCV)=6.70, 95%CI[5.64, 7.75], P<0.000 01; MD_(posterior tibial SCV)=4.03, 95%CI[0.56, 7.49], P=0.02). In terms of motor nerve conduction velocity(MCV), compared with the conditions before treatment, the conduction velocities of median nerve, peroneal nerve, and posterior tibial nerve in both groups increased after treatment(P<0.01). The experimental group was superior to the control group in improving MCV after treatment(P<0.05). In terms of average blood glucose change, there is no statistical significance of the improvement of average blood glucose between experimental group and the control group(MD=-0.15, 95%CI[-0.82, 0.51], P=0.65). In terms of overall response rate, the curative effect of the experimental group in the treatment of DPN was superior to that of the control group(RR=1.23, 95%CI[1.11, 1.37], P=0.000 1). In terms of adverse events, no obvious adverse events were reported in the included RCTs. In light of existing data and indexes, the clinical efficacy of Shuxuetong Injection combined with western medicine in the treatment of DPN may be superior to that of conventional western medicine. It can improve the clinical symptoms, SCV, MCV, and average blood glucose of DPN, with good safety. However, existing clinical trials are few and the quality is low. It is recommended to further verify the above conclusions with internationally recognized outcome indicators.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220303.501DOI Listing
June 2022

The spatially separated active sites for holes and electrons boost the radicals generation for toluene degradation.

J Hazard Mater 2022 Jun 13;437:129329. Epub 2022 Jun 13.

Research Center for Environmental and Energy Catalysis, Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 611731, China; Yangtze Delta Region Institute (Huzhou), University of Electronic Science and Technology of China, Huzhou 313000, China. Electronic address:

Hydroxyl (⸱OH) and superoxide (⸱O) radicals are the main drivers for photocatalysis in toluene degradation, but their generation mechanisms are still ambiguous due to the lack of direct evidence. The spatially separated active sites for holes and electrons can help to clarify the dynamic process of radicals generation. By performing theoretical calculations, it is demonstrated that the spatially separated active sites for holes and electrons on the BiOCO surface can be constructed by introducing oxygen vacancies in the [BiO] layer. HO and O molecules can be better adsorbed and activated at hole and electron active sites, separately. Accordingly, the pristine and defective BiOCO are prepared. The dynamic behavior of HO and O molecules at the matching active sites is revealed, which indicates the efficient adsorption of reactants and the substantial production of radicals. Significantly, the specificity of the spatially separated holes and electrons active sites for ⸱OH and ⸱O radicals generation, respectively, is demonstrated by in situ EPR with the HO vapor atmosphere. This work provides a design concept for unraveling reaction mechanisms to realize controllable radicals generation.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129329DOI Listing
June 2022

Effect of simulated earthquake loading on radon exhalation from uranium tailings dam.

Environ Sci Pollut Res Int 2022 Jun 17. Epub 2022 Jun 17.

School of Resource, Environment and Safety Engineering, University of South China, Hengyang, 421001, Hunan, China.

Uranium tailings sand will continuously release radon-222. When the external condition changes, the exhalation of radon will also change. Thus, radon is being recommended as a tracer for dam damage assessment. When an earthquake is simulated on the uranium tailings dam with a shaking table test and the change in radon concentration is measured, it is observed that the earthquake causes micro-fissures in the uranium tailings dam, which aggregate to form fractures. During the process, the radon concentration will climb dramatically, as will the radon exhalation rate. To verify that the radon monitoring date is accurate, the acceleration response, surface displacement, and interior displacement are all monitored. The results show that radon can be utilized as a tracer to evaluate uranium tailings dam damage.
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http://dx.doi.org/10.1007/s11356-022-20758-1DOI Listing
June 2022

The complete chloroplast genomes of two species (Orobanchaceae) from Southwest China.

Mitochondrial DNA B Resour 2022 10;7(6):971-973. Epub 2022 Jun 10.

Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, China.

We report the complete chloroplast genome (plastome) sequences of (147,087 bp) and (145,726 bp), endemic to southwestern China. Both plastomes have typical quadripartite structures with one large-single copy region, one small-single copy region, and two inverted repeat regions. Both plastome sequences contained 37 tRNA genes and eight rRNA genes, but they differed in the numbers of protein-coding genes: had 76 functional genes and 12 pseudogenes while had 74 functional genes and 13 pseudogenes. Phylogenetic analysis shows that and are closely related, then sister to in the family Orobanchaceae.
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http://dx.doi.org/10.1080/23802359.2022.2080018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196808PMC
June 2022

A robotic leg inspired from an insect leg.

Bioinspir Biomim 2022 Jun 14. Epub 2022 Jun 14.

School of Mechanical and Aerospace Engineering, Nanyang Technological University, N3.2-01-20, Singapore 639798, Singapore, 637460, SINGAPORE.

While most insect-inspired robots come with a simple tarsus such as a hemispherical foot tip, insect legs have complex tarsal structures and claws, which enable them to walk on complex terrain. Their sharp claws can smoothly attach and detach on plant surfaces by actuating a single muscle. Thus, installing insect-inspired tarsus on legged robots would improve their locomotion on complex terrain. This paper shows that the tendon-driven ball-socket structure provides the tarsus both flexibility and rigidity, which is necessary for the beetle to walk on a complex substrate such as a mesh surface. Disabling the tarsus' rigidity by removing the socket and elastic membrane of a tarsal joint, the claws could not attach to the mesh securely. Meanwhile, the beetle struggled to draw the claws out of the substrate when we turned the tarsus rigid by tubing. We then developed a cable-driven bio-inspired tarsus structure to validate the function of the tarsus as well as to show its potential application in the legged robot. With the tarsus, the robotic leg was able to attach and retract smoothly from the mesh substrate when performing a walking cycle.
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http://dx.doi.org/10.1088/1748-3190/ac78b5DOI Listing
June 2022

MrgX2-SNAP-tag/cell membrane chromatography model coupled with liquid chromatography-mass spectrometry for anti-pseudo-allergic compound screening in Arnebiae Radix.

Anal Bioanal Chem 2022 Jun 13. Epub 2022 Jun 13.

School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an, 710061, China.

Pseudo-allergic reactions (PARs) are IgE-independent hypersensitivity reactions. Mas-related G protein-coupled receptor-X2 (MrgX2) was proved the key receptor of PAR. The anti-pseudo-allergic compound discovery based on MrgX2 was of great value. Cell membrane chromatography (CMC) based on MrgX2 provides a convenient and effective tool in anti-pseudo-allergic compound screening and discovery, and further improvements of this method are still needed. In this work, SNAP-tag was introduced at C-terminal of Mas-related G protein-coupled receptor (MrgX2-SNAP-tag), and an MrgX2-SNAP-tag/CMC model was then conducted using CMC technique. Comparative experiments showed that the new model not only satisfied the good selectivity and specificity of screening but also exhibited more stable and longer life span than traditional MrgX2/CMC model. By coupling with HPLC-MS, two compounds were screened out from Arnebiae Radix and identified as shikonin and acetylshikonin. Nonlinear chromatography was performed to study the interactions between two screened compounds and MrgX2, and binding constant (K) of shikonin and acetylshikonin with MrgX2 were 2075.67 ± 0.34 M and 32201.36 ± 0.35 M, respectively. Furthermore, β-hexosaminidase and histamine release assay in vitro demonstrated that shikonin (1-5 μM) and acetylshikonin (2.5-10 μM) could both antagonize C48/80-induced allergic reaction. In conclusion, the MrgX2-SNAP-tag/CMC could be a reliable model for screening pseudo-allergy-related components from complex systems.
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http://dx.doi.org/10.1007/s00216-022-04167-4DOI Listing
June 2022

The Effect of Artificial Liver Support System on Prognosis of HBV-Derived Hepatorenal Syndrome: A Retrospective Cohort Study.

Dis Markers 2022 1;2022:3451544. Epub 2022 Jun 1.

Department of Infectious Disease, Zhejiang Hospital, Hangzhou, China.

Hepatorenal syndrome (HRS) could occur when patients get decompensated liver cirrhosis. Meanwhile, hepatitis B virus (HBV) infection raises the risk of mortality of the end-stage liver diseases. As the artificial liver support system (ALSS) has been applied in liver failure, whether ALSS could benefit HBV-derived HRS remains uncertain. We retrospectively enlisted eligible HRS patients and compared the baseline characteristics and prognosis between HBV-derived HRS and non-HBV-derived HRS. Furthermore, propensity score matching (PSM) and Cox regression analyses were used to assess the beneficial effect of ALSS on HBV-derived HRS. In addition, a stratified analysis was carried out according to the degree of acute kidney injury (AKI) and the number of organ failures to observe in which populations ALSS can obtain the most excellent therapeutic effect. 669 patients were diagnosed as HRS, including 298 HBV negative and 371 HBV positive. Baseline characteristics were different between patients with HBV positive and HBV negative. HBV-derived HRS has higher 28-day mortality, though without a statistical difference. After PSM, 50 patients treated with ALSS and 150 patients treated with standard medical treatment (SMT) constituted a new cohort for the following analysis. We found that ALSS could significantly benefit HRS patients ( = 0.025). Moreover, the median survival time of patients treated with ALSS was longer than those treated with SMT. INR, neutrophil percentage, and treatment with ALSS were independent predictive factors for short-term mortality in HBV-derived HRS. The stratified analysis showed that ALSS could reduce the 28-day mortality of patients with HBV-derived HRS, especially those in AKI stage 3 and with organ failure ≥ 2. Additionally, serum bilirubin was significantly lower after ALSS, and the alteration of INR and creatinine were independent predictive elements for the mortality of HBV-derived HRS. HBV-derived HRS is more severe than non-HBV-derived HRS and has a worse prognosis. ALSS could reduce the short-term mortality of patients with HBV-derived HRS, especially those in AKI stage 3 and with organ failure ≥ 2. INR and the change of creatinine and INR could predict the prognosis of HBV-derived HRS. ChiCTR2200060123.
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http://dx.doi.org/10.1155/2022/3451544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177308PMC
June 2022

Ultrasound elastography in the diagnosis of biliary atresia in pediatric surgery: a systematic review and meta-analysis of diagnostic test.

Transl Pediatr 2022 May;11(5):748-756

Department of Pediatric Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: Biliary atresia (BA) is one of the most fatal hepatobiliary diseases in infants and young children, and is easy to cause obstructive jaundice in infants and children, and liver transplantation in children. BA and infantile hepatitis syndrome are the main causes of cholestatic jaundice. The early clinical manifestations are extremely similar, and timely identification is difficult. Therefore, early and differential diagnosis of BA is crucial. At present, there are many imaging diagnosis methods for BA, such as ultrasound examination and nuclear magnetic resonance. Ultrasound elastography (USE) is the most popular method. However, the application of USE in BA is still in the exploratory stage.

Methods: PubMed, Web of Science, Embase, Cochrane Library, and other databases were searched for articles on USE diagnosis of pediatric surgical BA. Later, the articles were screened, and the included articles should meet the following requirements: the research objects were children with BA; the intervention measures were USE diagnosis; the control group was diagnosed by other methods than USE. RevMan5.3 was adopted for quality assessment, and forest maps and summary receiver operating characteristic (SROC) curves were constructed.

Results: Finally, seven articles covering 490 patients were included. There were 120 patients in the control group. The included articles showed good quality and there was no obvious bias. The area under the SROC curve (AUC) for USE diagnosis of BA was 0.93, the sensitivity was 0.93 (95% CI: 0.72-1.00), and the specificity was 0.95 (95% CI: 0.74-1.00).

Discussion: USE showed high diagnostic value for pediatric surgical biliary tract closure.
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http://dx.doi.org/10.21037/tp-22-159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173880PMC
May 2022
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