Publications by authors named "Hong Wang"

4,937 Publications

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Platelet-rich plasma protects human melanocytes from oxidative stress and ameliorates melanogenesis induced by UVB irradiation.

Biosci Biotechnol Biochem 2021 May 11. Epub 2021 May 11.

Department of physiotherapy, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong, China.

To investigate the role of Platelet-rich plasma(PRP) from different sources in alleviating oxidative stress and ameliorating melanogenesis in UVB-irradiated PIG1 cells. PIG1 cells were irradiated with 80 mJ/cm2 UVB prior to 1% PRP application and the following experiments were taken: The viability of UVB-irradiated PIG1 cells, cellular malondialdehyde (MDA) and reactive oxygen species (ROS)content, and activities of anti-oxidant enzymes. Western blotting was utilized to detect the expression level of proteins associated with melanin synthesis, apoptosis and DNA lesions. We found that PRP intervention promoted cell proliferation, reduced MDA and ROS content, increased the activities of series of anti-oxidant enzymes and alleviated DNA damages in UVB-damaged PIG1 cells. Of notes, PRP treatment inhibited UVB-induced melanogenesis via PI3K/Akt/GSK3β signal pathway. Therefore, we suppose PRP treatment exerts protective role through their anti-oxidation effect on UVB-damaged PIG1 cells and hinders melanogenesis induced by UVB irradiation.
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http://dx.doi.org/10.1093/bbb/zbab085DOI Listing
May 2021

Morphological characteristics and ecological risk assessment of nitrogen and phosphorus in the sediments of Futunxi watershed in Fujian province.

Environ Monit Assess 2021 May 10;193(6):335. Epub 2021 May 10.

School of Geography Science, Nanjing Normal University, Nanjing, 210023, Jiangsu, China.

The total amount and morphology of nitrogen and phosphorus in sediments have important environmental significance. The study of the ecological risk assessment of nitrogen and phosphorus in sediments is important to understand the environmental quality of water body in the basin. In this study, taking the Futunxi Basin of Fujian Province as an example, the single factor index and bioavailability coefficient method were used to evaluate the ecological risk of nitrogen and phosphorus in the sediments, and reveal the spatial change and environmental significance of the nitrogen and phosphorus forms. The results showed that different morphological components of the bio-available nitrogen were distributed as organic sulfide bound nitrogen (SOEF-N) > iron-manganese oxidized nitrogen (SAEF-N) > weak acid leached nitrogen (WAEF-N) > ion exchange nitrogen (IEF-N). The inorganic phosphorus included the highest proportion of metal oxide bound phosphorus (NaOH-P), followed by calcium bound phosphorus (HCl-P). The proportion of reduced phosphorus (BD-P) was even lower, and the proportion of weakly adsorbed phosphorus (NH4Cl-P) was the lowest. The results of single factor pollution index showed that the sediment nitrogen in the study area mainly caused moderate pollution and phosphorus mainly caused light pollution. By contrast, the results of the bioavailability index method indicated that nitrogen mainly caused light pollution, and phosphorus mainly caused clean pollution in sediments. Combined with the characteristics of social economy and environment in the study area, it can be inferred that the bioavailability index method based on comprehensive evaluation of total amount and morphology can better characterize the spatial change and ecological risk of nitrogen and phosphorus in sediments.
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http://dx.doi.org/10.1007/s10661-021-09106-xDOI Listing
May 2021

Behcet's disease manifesting as esophageal variceal bleeding: A case report.

World J Clin Cases 2021 Apr;9(12):2854-2861

Department of Gastroenterology, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China.

Background: Behcet's disease (BD) is a chronic disease characterized by oral and vulvar ulcers as well as eye and skin damage and involves multiple systems. It presents as an alternating process of repeated attacks and remissions. Esophageal venous rupture and bleeding caused by BD is rarely reported at home and abroad. This paper reports a case of bleeding from oesophageal varices caused by BD, aiming to provide an additional dimension for considering the cause of bleeding from esophageal varices in the future.

Case Summary: A 38-year-old female patient was admitted due to a gradual increase in shortness of breath and chest tightness after the activity, and was admitted to our hospital for treatment. After admission, relevant examinations showed that the patient had multiple blood clots. Four days after admission, she suddenly experienced massive hematemesis. Emergency esophagogastroduodenoscopy revealed bleeding from esophageal and gastric varices. The patient had no history of viral hepatitis or drinking habits, and no history of special genetic diseases or congenital vascular diseases. There is no obvious abnormality in liver function. After reviewing the medical history, it was found that the patient had recurred oral ulcers since childhood, ulcers were visible in the perineum during menstruation, and there was an intermittent red nodular rash and uveitis. The current skin acupuncture reaction is positive, combined with the evaluation of the external hospital and our hospital, the main diagnosis is BD. She received methylprednisolone, cyclophosphamide, immunomodulation, acid suppression, gastric protection, and anticoagulation and anti-infection treatments, and was discharged from the hospital. During the 1-year follow-up period, the patient did not vomit blood again.

Conclusion: This case highlights bleeding from esophageal varices caused by BD, aiming to provide an additional dimension concerning the cause of bleeding from esophageal varices in the future.
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http://dx.doi.org/10.12998/wjcc.v9.i12.2854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058672PMC
April 2021

Albumin-Based LL37 Peptide Nanoparticles as a Sustained Release System against Lung Infection.

ACS Biomater Sci Eng 2021 May 31;7(5):1817-1826. Epub 2020 Aug 31.

Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China.

(PA) has emerged as a pressing challenge to pulmonary infection and lung damage. The LL37 peptide is an efficient antimicrobial agent against PA strains, but its application is limited because of fast clearance , biosafety concerns, and low bioavailability. Thus, an albumin-based nanodrug delivery system with reduction sensitivity was developed by forming intermolecular disulfide bonds to increase LL37 performance against PA. Cationic LL37 can be efficiently encapsulated via electrostatic interactions to exert improved antimicrobial effects. The LL37 peptide exhibits greater than 48 h of sustained released from LL37 peptide nanoparticles (LL37 PNP), and prolonged antimicrobial effects were noted as the incubation time increased. Levels of inflammatory cytokines secreted by peritoneal macrophages, including TNF-α and IL-6, were reduced significantly after LL37 PNP treatment following PA stimulation, indicating that LL37 PNP inhibits PA growth and exerts anti-inflammatory effects . In a murine model of acute PA lung infection, LL37 PNP significantly reduced TNF-α and IL-1β expression and alleviated lung damage. The accelerated clearance of PA indicates that LL37 PNP could improve PA lung infection and the subsequent inflammation response more efficiently compared with free LL37 peptide. In conclusion, this excellent biocompatible LL37 delivery strategy may serve as an alternative approach for the application of new types of clinical treatment in future.
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http://dx.doi.org/10.1021/acsbiomaterials.0c01084DOI Listing
May 2021

Identification of pseudolaric acid B as a novel Hedgehog pathway inhibitor in medulloblastoma.

Biochem Pharmacol 2021 May 5:114593. Epub 2021 May 5.

Institute of Clinical Pharmacology, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China; Research Center of Chinese Herbal Resources Science and Engineering, School of Pharmaceutical Sciences, Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. Electronic address:

Aberrant activation of the Hedgehog (Hh) pathway is implicated in the pathogenesis and development of multiple cancers, especially Hh-driven medulloblastoma (MB). Smoothened (SMO) is a promising therapeutic target of the Hh pathway in clinical cancer treatment. However, SMO mutations frequently occur, which leads to drug resistance and tumor relapse. Novel inhibitors that target both the wild-type and mutant SMO are in high demand. In this study, we identified a novel Hh pathway inhibitor, pseudolaric acid B (PAB), which significantly inhibited the expression of Gli1 and its transcriptional target genes, such as cyclin D1 and N-myc, thus inhibiting the proliferation of DAOY and Ptch1 primary MB cells. Mechanistically, PAB can potentially bind to the extracellular entrance of the heptahelical transmembrane domain (TMD) of SMO, based on molecular docking and the BODIPY-cyclopamine binding assay. Further, PAB also efficiently blocked ciliogenesis, demonstrating the inhibitory effects of PAB on the Hh pathway at multiple levels. Thus, PAB may overcome drug-resistance induced by SMO mutations, which frequently occurs in clinical setting. PAB markedly suppressed tumor growth in the subcutaneous allografts of Ptch1 MB cells. Together, our results identified PAB as a potent Hh pathway inhibitor to treat Hh-dependent MB, especially cases resistant to SMO antagonists.
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http://dx.doi.org/10.1016/j.bcp.2021.114593DOI Listing
May 2021

Sensory-based approaches in psychiatric care: A systematic mixed-methods review.

J Adv Nurs 2021 May 5. Epub 2021 May 5.

School of Nursing, Jilin University, Jilin, People's Republic of China.

Aims: Sensory-based approaches, including sensory room, sensory cart and specific sensory integration programs, feature various sensory stimulations to focus on a particular space or program. This systematic mixed-methods review describes the impact of sensory-based approaches in psychiatric care and summarizes the important components of sensory interventions.

Design: Systematic mixed-methods review was based on the guidelines by Pluye and Hong for comprehensively searching, appraising and synthesizing research evidence.

Data Sources: Data were collected from five databases: CINAHL, Embase, Pubmed, Web of Science and Cochrane before March 9, 2020.

Review Methods: Qualitative, quantitative, mixed-methods and original studies published in English on sensory-based approaches in psychiatric care were included. The studies were selected by screening titles, abstracts and full texts, and the quality of each study was assessed by two researchers independently. The data were analysed using thematic analysis.

Results: Sixteen studies were chosen for review. Through data integration, four subthemes with positive effects were formed: (1) calming of the patient's mood; (2) calming of the patient's body; (3) improvement of self-care ability; and (4) improvement of the nurse-patient relationship. The sensory-based approach may also lead to negative effects.

Conclusions: Several important components play important roles in the sensory-based approaches: (1) rich, culture-based, personalized sensory stimulation; (2) a quiet, safe, home-based physical environment; (3) a good one-to-one nurse-patient relationship; (4) and the cultivation of patient autonomy and self-management. Sensory-based approaches in a multicultural environment and home environment will be important topics of psychiatric care in the future.

Impact: There is a lack of synthesis of studies on results of sensory-based approaches in psychiatric care. Four components are important to sensory interventions. It is necessary for mental health service centres and home care for the patients with psychotic disorders to use sensory-based approaches for reference.
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http://dx.doi.org/10.1111/jan.14884DOI Listing
May 2021

Proteomic analysis identifies key differences in the cardiac interactomes of dystrophin and micro-dystrophin.

Hum Mol Genet 2021 May 5. Epub 2021 May 5.

Centre for Gene Therapy, The Research Institute at Nationwide Children's Hospital, Columbus, 43205, OH, USA.

ΔR4-R23/ΔCT micro-dystrophin (μDys) is a miniaturized version of dystrophin currently evaluated in a Duchenne muscular dystrophy (DMD) gene therapy trial to treat skeletal and cardiac muscle disease. In pre-clinical studies, μDys efficiently rescues cardiac histopathology, but only partially normalizes cardiac function. To gain insights into factors that may impact the cardiac therapeutic efficacy of μDys, we compared by mass spectrometry the composition of purified dystrophin and μDys protein complexes in the mouse heart. We report that compared to dystrophin, μDys has altered associations with α1- and β2-syntrophins, as well as cavins, a group of caveolae-associated signaling proteins. In particular, we found that membrane localization of cavins -1 and - 4 in cardiomyocytes requires dystrophin and is profoundly disrupted in the heart of mdx5cv mice, a model of DMD. Following cardiac stress/damage, membrane-associated cavin-4 recruits the signaling molecule ERK to caveolae, which activates key cardio-protective responses. Evaluation of ERK signaling revealed a profound inhibition, below physiological baseline, in the mdx5cv mouse heart. Expression of μDys in mdx5cv mice prevented the development of cardiac histopathology but did not rescue membrane localization of cavins nor did it normalize ERK signaling. Our study provides the first comparative analysis of purified protein complexes assembled in vivo by full-length dystrophin and a therapeutic micro-dystrophin construct. This has revealed disruptions in cavins and ERK signaling that may contribute to DMD cardiomyopathy. This new knowledge is important for ongoing efforts to prevent and treat heart disease in DMD patients.
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http://dx.doi.org/10.1093/hmg/ddab133DOI Listing
May 2021

Advances of α-activated cyclic isothiocyanate for the enantioselective construction of spirocycles.

Org Biomol Chem 2021 May 5. Epub 2021 May 5.

College of Pharmaceutical Science & Collaborative Innovation Centre of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, China.

The efficient and enantioselective synthesis of pharmaceutically important spirocycles has attracted the focus of organic and medicinal chemists. In this context, with the excellent reactivity of α-activated isothiocyanate as formal 1,3-dipoles in the (3 + 2) cyclization process, the cyclic isothiocyanates featuring important pharmacophores, such as oxindole, pyrazolone, and indanone moieties, have emerged as powerful precursors to access a variety of spirocycles with highly structural diversities. In addition, the facile transformations of these spirocycles have shown potential applications in drug design. This review will cover the recent advances of α-activated cyclic isothiocyanates in the enantioselective construction of spirocycles since 2015, and the applications of corresponding products in organic and medicinal chemistry.
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http://dx.doi.org/10.1039/d1ob00564bDOI Listing
May 2021

A turn-on homodimer fluorescent probe based on homo-FRET for the sensing of biothiols in lysosome: a trial of a new turn-on strategy.

Analyst 2021 May;146(9):2974-2982

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

Fluorescence resonance energy transfer (FRET) is often applied to construct fluorescent probes for acquiring high selectivity and sensitivity. According to the FRET theory, a homodimer composed of two identical fluorophores with a small Stokes shift has only weak fluorescence due to homo-FRET between fluorophores, and the fluorescence could be recovered after the destruction of the homodimer. In this study, we designed and synthesized a homodimer fluorescent probe, namely 1,3,5,7-tetramethyl-8-(4'-phenylthiophenol)-boron difluoride-dipyrrole methane dimer (D-TMSPB), based on this turn-on strategy. In D-TMSPB, the disulfide moiety was selected as the response moiety of biothiols, and BODIPY fluorophore was chosen as both donor and acceptor in FRET due to the ultra-small Stokes shifts and obvious overlap of its excitation/emission peak. D-TMSPB exhibited only weak fluorescence. After selective reaction with biothiols, FRET was destroyed and the derivative exhibited strong fluorescence at 514 nm with the limit of detection of about 0.15 μM for GSH. Notably, the derivative of biothiols shows remarkable fluorescence only in acidic conditions, which accords with the internal environment of lysosome. Thus, D-TMSPB was applied to image the biothiols of lysosome in living cells. The turn-on fluorescence of D-TMSPB indicated that homo-FRET is a practical strategy to design turn-on fluorescent probes, particularly for the sensing mechanism based on leaving groups.
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http://dx.doi.org/10.1039/d1an00161bDOI Listing
May 2021

Immune Regulatory Roles of Cells Expressing Taste Signaling Elements in Nongustatory Tissues.

Handb Exp Pharmacol 2021 May 5. Epub 2021 May 5.

Monell Chemical Senses Center, Philadelphia, PA, USA.

G protein-coupled taste receptors and their downstream signaling elements, including Gnat3 (also known as α-gustducin) and TrpM5, were first identified in taste bud cells. Subsequent studies, however, revealed that some cells in nongustatory tissues also express taste receptors and/or their signaling elements. These nongustatory-tissue-expressed taste receptors and signaling elements play important roles in a number of physiological processes, including metabolism and immune responses. Special populations of cells expressing taste signaling elements in nongustatory tissues have been described as solitary chemosensory cells (SCCs) and tuft cells, mainly based on their morphological features and their expression of taste signaling elements as a critical molecular signature. These cells are typically scattered in barrier epithelial tissues, and their functions were largely unknown until recently. Emerging evidence shows that SCCs and tuft cells play important roles in immune responses to microbes and parasites. Additionally, certain immune cells also express taste receptors or taste signaling elements, suggesting a direct link between chemosensation and immune function. In this chapter, we highlight our current understanding of the functional roles of these "taste-like" cells and taste signaling pathways in different tissues, focusing on their activities in immune regulation.
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http://dx.doi.org/10.1007/164_2021_468DOI Listing
May 2021

Development of a group-specific antibody-based immunoassay method for simultaneously detecting sildenafil-like adulterants in herbal spirit drinks.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 May 1:1-12. Epub 2021 May 1.

College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, P. R. China.

Phosphodiesterase type 5 (PDE-5) inhibitors are commonly used to treat erectile dysfunction. There is a problem with synthesis and illegal use of a wide range of analogues of the licenced drugs and a simple class-wide analytical method is required. In this work, based on structural modelling, we developed an immunological method using norneovardenafil as a hapten as it contains only the general sub-structure and the common features of sildenafil-like adulterants, such as hydrophobic centres, hydrogen-bond donor atoms and hydrogen-bond acceptor atoms. Thus theoretically it could induce production of antibody which could recognise multiple sildenafil-like adulterants. By immunising rabbits, a group-specific polyclonal antibody was obtained with the desired broad-spectrum molecular recognition performance against sildenafil-like adulterants. Then, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed for the detection of sildenafil-like adulterants in herbal spirit drinks. Under the optimised conditions, the icELISA method showed broad linear ranges for acetildenafil, sildenafil and vardenafil respectively of 0.7 to 27.7 μg/kg, 1.0 to 70.7 μg/kg and 1.5 to 22.7 μg/kg, with half-maximal inhibition concentration (IC) values of 4.5 μg/kg, 8.3 μg/kg and 5.7 μg/kg, respectively. For eleven herbal spirit drinks, there was good agreement between total levels of sildenafil-like adulterants measured by icELISA and levels of each of four individual adulterants determined by LC-MS/MS. In short, the developed icELISA can be employed for rapid and simple screening for adulteration of herbal spirit drinks with sildenafil-like compounds.
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http://dx.doi.org/10.1080/19440049.2021.1905185DOI Listing
May 2021

Association of the rs17250932, rs4794067, and rs2240017 polymorphism in the TBX21 gene with autoimmune diseases: A meta-analysis.

Allergol Immunopathol (Madr) 2021 1;49(3):83-90. Epub 2021 May 1.

Department of Epidemiology and Biostatistics, School of Public Health and Anhui Province Laboratory of Inflammation and Immune Mediated Diseases, Anhui Medical University, Hefei, Anhui, P.R. China;

Objective: To evaluate systematically the association between gene polymorphisms (rs17250932, rs2240017, and rs4794067) and the risk of autoimmune diseases in Asian populations.

Methods: The Medline, Web of Science, and Chinese Biomedical Literature Database were used to retrieve eligible studies that were published before July 2020. Pooled odds ratios (OR) and 95% confidence intervals (95% CI) were calculated by using the dominant model, heterozygote contrast model, and allelic contrast model. Publication bias was evaluated using contour-enhanced funnel plots and Egger's regression test. Sensitivity analysis was conducted to assess the robustness of this meta-analysis.

Results: A total of 12 eligible studies, including 3834 patients and 4824 healthy controls, were recruited in this meta-analysis. The pooled data demonstrated that rs2240017 and rs4794067 polymorphisms were significantly associated with the risk of autoimmune diseases in Asian populations in allelic contrast model (OR: 1.456, 95% CI: 1.131-1.875, P = 0.004; OR: 0.766, 95% CI: 0.615-0.954, P = 0.017), heterozygote comparison model (OR: 1.647, 95% CI: 1.239-2.189, P = 0.001; OR: 0.796, 95% CI: 0.634-0.999, P = 0.049), and dominant model (OR: 1.572, 95% CI: 1.194-2.071, P = 0.004; OR: 0.767, 95% CI: 0.607-0.970, P = 0.027). The G allele of rs2240017 may be a risk factor for autoimmune diseases, and the T allele of rs4794067 may increase the risk of autoimmune diseases. However, we failed to find evidence of the association between rs17250932 polymorphism and susceptibility to autoimmune diseases. No publication bias was established in this meta-analysis.

Conclusion: This meta-analysis indicated that rs2240017 and rs4794067 polymorphism confer susceptibility to autoimmune diseases, but not rs17250932.
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http://dx.doi.org/10.15586/aei.v49i3.80DOI Listing
May 2021

Postoperative morbidity adversely impacts long-term oncologic prognosis following hepatectomy for hepatocellular carcinoma: A multicenter observational study.

Eur J Surg Oncol 2021 Apr 26. Epub 2021 Apr 26.

Department of Hepatobiliary Pancreatic and Minimal Invasive Surgery, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China; School of Clinical Medicine, Hangzhou Medical College, Hangzhou, Zhejiang, China; Department of Hepatobiliary Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Navy Medical University, Shanghai, China. Electronic address:

Background & Aims: Postoperative morbidity following hepatectomy for hepatocellular carcinoma (HCC) is common and its impact on long-term oncological outcome remains unclear. This study aimed to investigate if postoperative morbidity impacts long-term survival and recurrence following hepatectomy for HCC.

Methods: The data from a multicenter Chinese database of curative-intent hepatectomy for HCC were analyzed, and independent risks of postoperative 30-day morbidity were identified. After excluding patients with postoperative early deaths (≤90 days), early (≤2 years) and late (>2 years) recurrence rates, overall survival (OS), and time-to-recurrence (TTR) were compared between patients with and without postoperative morbidity.

Results: Among 2,161 patients eligible for the study, 758 (35.1%) had postoperative 30-day morbidity. Multivariable logistic regression analysis showed that diabetes mellitus, obesity, Child-Pugh grade B, cirrhosis, and intraoperative blood transfusion were independent risks of postoperative morbidity. The rates of early and late recurrence among patients with postoperative morbidity were higher than those without (50.7% vs. 38.8%, P < 0.001; and 41.7% vs. 34.1%, P = 0.017). Postoperative morbidity was associated with decreased OS (median: 48.1 vs. 91.6 months, P < 0.001) and TTR (median: 19.8 vs. 46.1 months; P < 0.001). After adjustment of confounding factors, multivariable Cox-regression analyses revealed that postoperative morbidity was associated with a 27.8% and 18.7% greater likelihood of mortality (hazard ratio 1.278; 95% confidence interval: 1.126-1.451; P < 0.001) and recurrence (1.187; 1.058-1.331; P = 0.004).

Conclusion: This large multicenter study provides strong evidence that postoperative morbidity adversely impacts long-term oncologic prognosis after hepatectomy for HCC. The prevention and management of postoperative morbidity may be oncologically important.
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http://dx.doi.org/10.1016/j.ejso.2021.04.027DOI Listing
April 2021

Identifying reference values for serum lipids in Chinese children and adolescents aged 6-17 years old: A national multicenter study.

J Clin Lipidol 2021 Feb 27. Epub 2021 Feb 27.

Xiangya School of Public Health, Central South University, No.238 Shang Ma Yuan Ling Road, Changsha 410008, China. Electronic address:

Background: Current reference values for pediatric dyslipidemia used in China were not developed based on local population studies and did not consider age and sex differences.

Objective: In this study, we aimed to determine suitable reference values for total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), and non-high-density lipoprotein cholesterol (nonHDL-C) for Chinese children and adolescents using a national multicenter school-based study.

Methods: A total of 15,830 students aged 6-17 years were recruited from seven provinces of China. Age- and sex-specific percentile values for each lipid indicator were derived based on levels measured in the fasting state, and percentile curves of each indicator were plotted using the LMS method.

Results: Serum lipid levels varied considerably with age in both sexes. Among boys, the cut-off value for high TC, nonHDL-C, LDL-C, and TG, based on the value of the 95th percentiles, ranged from 4.58 to 5.39, 3.34 to 3.99, 2.69 to 3.31, and 1.22 to 1.83 mmol/L, respectively; among girls, the cut-off value for high TC, nonHDL-C, LDL-C, and TC ranged from 5.01 to 5.39, 3.66 to 3.97, 2.97 to 3.32, and 1.41 to 1.93 mmol/L, respectively. The cut-point for low HDL-C ranged from 0.84 to 1.08 mmol/L in boys and from 0.89 to 1.04 mmol/L in girls.

Conclusion: These findings may help to determine age- and sex-specific reference values for serum lipids among Chinese children and adolescents and provide valuable guidance for screening of dyslipidemia.
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http://dx.doi.org/10.1016/j.jacl.2021.02.001DOI Listing
February 2021

Multifunctional pathology-mapping theranostic nanoplatforms for US/MR imaging and ultrasound therapy of atherosclerosis.

Nanoscale 2021 Apr 30. Epub 2021 Apr 30.

Department of Ultrasound, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.

Atherosclerotic thrombosis is the leading cause of most life-threatening cardiovascular diseases (CVDs), particularly as a result of rupture or erosion of vulnerable plaques. Rupture or erosion-prone plaques are quite different in cellular composition and immunopathology, requiring different treatment strategies. The current imaging technology cannot distinguish the types of vulnerable plaques, and thus empirical treatment is still applied to all without a tailored and precise treatment. Herein, we propose a novel strategy called "Multifunctional Pathology-mapping Theranostic Nanoplatform (MPmTN)" for the tailored treatment of plaques based on the pathological classification. MPmTNs are made up of poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), containing contrast imaging materials Fe3O4 and perfluoropentane (PFP), and coated with specific plaque-targeted peptides PP1 and cyclic RGD. The PFP encapsulated inside the MPmTN can undergo a phase change from nanodroplets to gas microbubbles under therapeutic ultrasound (TUS) exposure. The acoustic and biological effects induced by TUS and disruption of microbubbles may further promote therapeutic effects. Hypothetically, MPmTN NPs can target the rupture-prone plaque via the binding of PP1 to class A scavenger receptors (SR-A) on macrophages, induce the apoptosis due to TUS exposure and thus reduce the chronic soakage of inflammatory cells. The MPmTN NPs can also target the erosion-prone plaque through the binding of cRGD to glycoprotein (GP) IIb/IIIa on activated platelets and promote platelet disaggregation under TUS exposure. Therefore, MPmTNs may work as a multifunctional pathology-mapping therapeutic agent. Our in vitro results show that the MPmTN with PP1 and cRGD peptides had a high binding affinity both for activated macrophages and blood clots. Under TUS exposure, the MPmTN could effectively induce macrophage apoptosis, destroy thrombus and exhibit good imaging properties for ultrasound (US) and MRI. In apoE-/- mice, MPmTNs can selectively accumulate at the plaque site and reduce the T2-weighted signal. The apoptosis of macrophages and disaggregation of activated platelets on the plaques were also confirmed in vivo. In summary, this study provides a potential strategy for a tailored treatment of vulnerable plaques based on their pathological nature and a multimodal imaging tool for the risk stratification and assessment of therapeutic efficacy.
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http://dx.doi.org/10.1039/d1nr01096dDOI Listing
April 2021

Targeting Antisense lncRNA PRKAG2-AS1, as a Therapeutic Target, Suppresses Malignant Behaviors of Hepatocellular Carcinoma Cells.

Front Med (Lausanne) 2021 13;8:649279. Epub 2021 Apr 13.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Army Medical University, Chongqing, China.

Increasing evidence highlights antisense long non-coding RNAs (lncRNAs) as promising therapeutic targets for cancers. Herein, this study focused on the clinical implications and functions of a novel antisense lncRNA PRKAG2-AS1 in hepatocellular carcinoma (HCC). PRKAG2-AS1 expression was examined in a cohort of 138 HCC patients by RT-qPCR. Overall survival (OS) and disease-free survival (DFS) analyses were presented based on PRKAG2-AS1 expression, followed by ROCs. After silencing PRKAG2-AS1, cell proliferation was assessed via CCK-8, colony formation and EdU staining assays. Migrated and invasive capacities were assessed by wound healing and transwell assays. The relationships between PRKAG2-AS1, miR-502-3p and BICD2 were validated by luciferase reporter, RIP and RNA pull-down assays. The expression and prognostic value of BICD2 were analyzed in TCGA database. PRKAG2-AS1 was up-regulated in HCC than normal tissue specimens. High PRKAG2-AS1 expression was indicative of poorer OS and DFS time. Area under the curves (AUCs) for OS and DFS were 0.8653 and 0.7891, suggesting the well predictive efficacy of PRKAG2-AS1 expression. Targeting PRKAG2-AS1 distinctly inhibited proliferation, migration, and invasion in HCC cells. PRKAG2-AS1 was mainly expressed in cytoplasm of HCC cells. PRKAG2-AS1 may directly bind to the sites of miR-502-3p. Up-regulation of BICD2 was found in HCC tissues and associated with unfavorable prognosis. BICD2 was confirmed to be a downstream target of miR-502-3p. PRKAG2-AS1 could regulate miR-502-3p/BICD2 axis. Our findings identified a novel lncRNA PRKAG2-AS1 that was associated with clinical implications and malignant behaviors. Thus, PRKAG2-AS1 could become a promising therapeutic target.
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http://dx.doi.org/10.3389/fmed.2021.649279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076551PMC
April 2021

Study on the Sleep-Improvement Effects of Baroni in and Targeted Screening to Identify Its Active Components and Mechanism.

Foods 2021 Apr 17;10(4). Epub 2021 Apr 17.

College of Food Science, South China Agricultural University, Guangzhou 510642, China.

Baroni (HC) is an edible plant in Asia, and it has been traditionally used for sleep-improvement. However, the bioactive components and mechanism of HC in sleep-improvement are still unclear. In this study, the sleep-improvement effect of HC hydroalcoholic extract was investigated based on a caffeine-induced insomnia model in (), and the ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-ESI-Orbitrap-MS) and network pharmacology strategy were further combined to screen systematically the active constituents and mechanism of HC in sleep-improvement. The results suggested HC effectively regulated the number of nighttime activities and total sleep time of in a dose-dependent manner and positively regulated the sleep bouts and sleep duration of . The target screening suggested that quercetin, luteolin, kaempferol, caffeic acid, and nicotinic acid were the main bioactive components of HC in sleep-improvements. Moreover, the core targets (Akt1, Cat, Ple, and Sod) affected by HC were verified by the expression of the mRNA of . In summary, this study showed that HC could effectively regulate the sleep of and further clarifies the multi-component and multi-target features of HC in sleep-improvement, which provides a new insight for the research and utilization of HC.
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http://dx.doi.org/10.3390/foods10040883DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072781PMC
April 2021

Difference in gene mutation profile in patients with refractory/relapsed versus newly diagnosed acute myeloid leukemia based on targeted next-generation sequencing.

Leuk Lymphoma 2021 Apr 29:1-12. Epub 2021 Apr 29.

Department of Hematology, Chinese PLA General Hospital, Beijing, China.

We have reported the genetic mutation profile in previously untreated acute myeloid leukemia (AML) patients using a targeted NGS screening method. In this study, we evaluated the characteristics and prognostic significance of gene mutations in refractory/relapsed (R/R) AML patients by comparing their gene mutation spectrum to those newly diagnosed. The frequencies of tumor suppressor mutations were increased, while the mutation frequencies of nucleophosmin and spliceosome complex were decreased in relapsed AML. The frequency of FLT3-ITD mutation was increased, while that of CEBPA biallelic mutation decreased in refractory AML. Activated signaling mutations predicted a lower complete remission rate. FLT3-ITD mutation predicted an inferior overall survival after relapse. DNMT3A mutation predicted an inferior relapse-free survival in R/R AML. These findings may shed light on the molecular mechanism study of leukemia refractory or relapse and provide new guidance for the dynamic risk assessment of AML.
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http://dx.doi.org/10.1080/10428194.2021.1919661DOI Listing
April 2021

Effects of Shuanghuanglian oral liquids on patients with COVID-19: a randomized, open-label, parallel-controlled, multicenter clinical trial.

Front Med 2021 Apr 28. Epub 2021 Apr 28.

Division of Cardiology, Department of Internal Medicine and Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

We conducted a randomized, open-label, parallel-controlled, multicenter trial on the use of Shuanghuanglian (SHL), a traditional Chinese patent medicine, in treating cases of COVID-19. A total of 176 patients received SHL by three doses (56 in low dose, 61 in middle dose, and 59 in high dose) in addition to standard care. The control group was composed of 59 patients who received standard therapy alone. Treatment with SHL was not associated with a difference from standard care in the time to disease recovery. Patients with 14-day SHL treatment had significantly higher rate in negative conversion of SARS-CoV-2 in nucleic acid swab tests than the patients from the control group (93.4% vs. 73.9%, P = 0.006). Analysis of chest computed tomography images showed that treatment with high-dose SHL significantly promoted absorption of inflammatory focus of pneumonia, which was evaluated by density reduction of inflammatory focus from baseline, at day 7 (mean difference (95% CI), -46.39 (-86.83 to -5.94) HU; P = 0.025) and day 14 (mean difference (95% CI), -74.21 (-133.35 to -15.08) HU; P = 0.014). No serious adverse events occurred in the SHL groups. This study illustrated that SHL in combination with standard care was safe and partially effective for the treatment of COVID-19.
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http://dx.doi.org/10.1007/s11684-021-0853-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079840PMC
April 2021

Schisandrin B inhibits epithelial‑mesenchymal transition and stemness of large‑cell lung cancer cells and tumorigenesis in xenografts via inhibiting the NF‑κB and p38 MAPK signaling pathways.

Oncol Rep 2021 Jun 28;45(6). Epub 2021 Apr 28.

Department of Anesthesiology, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China.

Lung cancer is one of the most common types of cancer in the world, resulting in numerous cancer‑associated deaths. The properties of cancer stem cells (CSCs) are important for the initiation and deterioration of lung cancer. Schisandrin B (SchB), an active compound extracted from , exerts anticancer effects in various malignancies, including lung cancer. Nevertheless, the potential of SchB in epithelial‑mesenchymal transition (EMT) and CSC features of large‑cell lung cancer remains unclear. The present study established cancer stem‑like cells derived from large‑cell lung cancer cells, NCI‑H460 and H661, and revealed that SchB inhibited the viability of cancer stem‑like cells at concentrations of ≥40 µmol/l. Moreover, SchB prominently inhibited cell migration, invasion and EMT. Sphere‑forming assays and western blotting demonstrated that the stemness of cancer stem‑like cells was alleviated by SchB treatment. Mechanistically, the current findings revealed that SchB contributed to the suppression of the NF‑κB and p38 MAPK signaling pathways. Notably, further results revealed that the malignant behaviors of NCI‑H460‑CSCs induced by the activation of the NF‑κB and p38 MAPK signaling pathways were suppressed by SchB treatment. Consistently, the inhibitory role of SchB in EMT and CSC activities, as well as in the activation of the NF‑κB and p38 MAPK signaling pathways, was confirmed . In conclusion, the present study demonstrated that SchB exerted inhibitory effects on large‑cell lung cancer cells via targeting the NF‑κB and p38 MAPK signaling pathways, suggesting that SchB may act as a potential therapeutic drug for large‑cell lung cancer.
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http://dx.doi.org/10.3892/or.2021.8066DOI Listing
June 2021

[Evaluation of the effect of 3D printed HAP-GEL scaffold combined with BMSCs and HUVECs in repairing rabbit skull defect].

Shanghai Kou Qiang Yi Xue 2021 Feb;30(1):28-32

Department of Stomatology, Weifang Medical University. Weifang 261053, China.

Purpose: To qualitatively analyze the effect of 3D printed hydroxyapatite-gel (HAP-GEL) scaffold combined with bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) in repairing rabbit skull defect.

Methods: The third generation BMSCs and HUVECs were co-cultured with 3D printed HAP-GEL scaffold to construct tissue engineering bone. The rabbit model of skull defect was established and randomly divided into 4 groups. HAP-GEL stent, HAP-GEL stent + BMSCs and HUVECs cells were implanted respectively, and positive control (autologous bone tissue) and blank control were set up. Twelve weeks after operation, X-ray, cone-beam CT (CBCT) scan and H-E staining were performed to observe and analyze the changes of bone defect qualitatively. RESULTS:Twelve weeks after operation, imaging examination (X-ray and CBCT) showed that there was still obvious circular transmission in the blank control group, and the density was increased and the defect boundary was blurred in both HAP-GEL stent combined cell group and HAP-GEL group, among which the bone was continuous and the bone mineral density was the highest in HAP-GEL stent composite cell group, which was close to normal tissue. The results of H-E staining at twelve weeks showed that compared with the blank control group and the HAP-GEL group, the defect area of the HAP-GEL composite group was filled with new bone and bone-like tissue, the scaffold material was degraded and there was new bone formation inside the scaffold, and the bone repair effect was good, and the osteogenic effect was similar to that of the positive control group.

Conclusions: 3D printed HAP-GEL scaffold + BMSCs + HUVECs cell complex has good osteogenic ability and biocompatibility with a good effect on repairing rabbit skull defect.
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February 2021

Alisol A 24-acetate protects oxygen-glucose deprivation-induced brain microvascular endothelial cells against apoptosis through miR-92a-3p inhibition by targeting the gene.

Pharm Biol 2021 Dec;59(1):513-524

The Affiliated Rehabilitation Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou, China.

Context: Alisol A 24-acetate has been used to treat vascular diseases. However, the underlying mechanisms still remain unclear.

Objective: The present study evaluated the antiapoptotic effect of alisol A 24-acetate on brain microvascular endothelial cells (BMECs) and explored the underlying mechanisms.

Materials And Methods: BMECs were injured through oxygen -glucose deprivation (OGD) after alisol A 24-acetate treatment. Cell viability and half-maximal inhibitory concentration (IC) were measured using CCK-8, whereas inflammatory factors and oxidative stress indicators were measured using enzyme linked immunosorbent assay. Cell invasion and wound healing assays were detected. Cell apoptosis was assessed using flow cytometry. B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X (Bax) expression were analyzed using Western blotting. Dual-luciferase assay was applied to detect target genes of miR-92a-3p.

Result: Alisol A 24-acetate had an IC of 98.53 mg/L and inhibited cell viability at concentrations over 50mg/L. OGD induced apoptosis and promoted miR-92a-3p overexpression in BMECs. However, alisol A 24-acetate treatment suppressed inflammation, improved migration and invasion abilities, increased Bcl-2 expression, inhibited Bax expression, and repressed apoptosis and miR92a-3p overexpression in OGD-induced BMECs. MiR-92a-3p overexpression promoted cell apoptosis and suppressed Bcl-2 expression, whereas its inhibitor reversed the tendency. Alisol A 24-acetate treatment relieved the effects of miR-92a-3p overexpression. Dual-luciferase assay confirmed that miR-92a-3p negatively regulated the Bcl-2 expression.

Conclusions: These findings suggest that alisol A 24-acetate exerts antiapoptotic effects on OGD-induced BMECs through miR-92a-3p inhibition by targeting the Bcl-2 gene, indicating its potential for BMECs protection and as a novel therapeutic agent for the treatment of cerebrovascular disease.
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http://dx.doi.org/10.1080/13880209.2021.1912117DOI Listing
December 2021

Orthogonal Demethylase-Activated Deoxyribozyme for Intracellular Imaging and Gene Regulation.

J Am Chem Soc 2021 May 27;143(18):6895-6904. Epub 2021 Apr 27.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.

The epigenetic modification of nucleic acids represents a versatile approach for achieving high-efficient control over gene expression and transcription and could dramatically expand their biosensing and therapeutic applications. Demethylase-involved removal of N6-methyladenine (mA) represents one of the vital epigenetic reprogramming events, yet its direct intracellular evaluation and as-guided gene regulation are extremely rare. The endonuclease-mimicking deoxyribozyme (DNAzyme) is a catalytically active DNA that enables the site-specific cleavage of the RNA substrate, and several strategies have imparted the magnificent responsiveness to DNAzyme by using chemical and light stimuli. However, the epigenetic regulation of DNAzyme has remained largely unexplored, leaving a significant gap in responsive DNA nanotechnology. Herein, we reported an epigenetically responsive DNAzyme system through the selection of an exquisite mA-caged DNAzyme that could be specifically activated by FTO (fat mass and obesity-associated protein) demethylation for precise intracellular imaging-directed gene regulation. Based on a systematic investigation, the active DNAzyme configuration was potently disrupted by the site-specific incorporation of mA modification and subsequently restored into the intact DNAzyme structure via the tunable FTO-specific removal of mA-caging groups under a variety of conditions. This orthogonal demethylase-activated DNAzyme amplifier enables the robust and accurate monitoring of FTO and its inhibitors in live cells. Moreover, the simple demethylase-activated DNAzyme facilitates the assembly of an intelligent self-adaptive gene regulation platform for knocking down demethylase with the ultimate apoptosis of tumor cells. As a straightforward and scarless mA removal strategy, the demethylase-activated DNAzyme system offers a versatile toolbox for programmable gene regulation in synthetic biology.
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http://dx.doi.org/10.1021/jacs.1c00570DOI Listing
May 2021

Characteristics of the extracellular products of pure oxygen aerated activated sludge in batch mode.

Water Sci Technol 2021 Apr;83(8):1946-1957

School of the Environment, Nanjing Normal University, Nanjing 210023, China E-mail: Key Laboratory of Virtual Geographic Environment (Nanjing Normal University), Ministry of Education, Nanjing 210023, China; State Key Laboratory Cultivation Base of Geographical Environment Evolution (Jiangsu Province), Nanjing 210023, China and Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023, China.

The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode. The structure of the extracellular products would change with different aeration methods or aeration rates. The proportion of SMP of most oxygen aerated sludge was less than 10%, while that in air aerated sludge was as high as 30-40%. The proportion of TB-EPS decreased from 56.95% to 30.63%, and the proportion of LB-EPS increased obviously with the increase of oxygen aeration rate. The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05). The zeta potential of each component in activated sludge was negative, gradually decreasing with the progress of biological treatment. The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions. The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.
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http://dx.doi.org/10.2166/wst.2021.099DOI Listing
April 2021

Direct Imaging of Integrated Circuits in CPU with 60 nm Super-Resolution Optical Microscope.

Nano Lett 2021 May 27;21(9):3887-3893. Epub 2021 Apr 27.

Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

Far-field super-resolution optical microscopies have achieved incredible success in life science for visualization of vital nanostructures organized in single cells. However, such resolution power has been much less extended to material science for inspection of human-made ultrafine nanostructures, simply because the current super-resolution optical microscopies modalities are rarely applicable to nonfluorescent samples or unlabeled systems. Here, we report an antiphase demodulation pump-probe (DPP) super-resolution microscope for direct optical inspection of integrated circuits (ICs) with a lateral resolution down to 60 nm. Because of the strong pump-probe (PP) signal from copper, we performed label-free super-resolution imaging of multilayered copper interconnects on a small central processing unit (CPU) chip. The label-free super-resolution DPP optical microscopy opens possibilities for easy, fast, and large-scale electronic inspection in the whole pipeline chain for designing and manufacturing ICs.
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http://dx.doi.org/10.1021/acs.nanolett.1c00403DOI Listing
May 2021

A urine-based DNA methylation assay to facilitate early detection and risk stratification of bladder cancer.

Clin Epigenetics 2021 Apr 26;13(1):91. Epub 2021 Apr 26.

AnchorDx Medical Co., Ltd, Unit 502, 3rd Luoxuan Road, International Bio-Island, Guangzhou, 510300, China.

Background: Current non-invasive tests have limited sensitivities and lack capabilities of pre-operative risk stratification for bladder cancer (BC) diagnosis. We aimed to develop and validate a urine-based DNA methylation assay as a clinically feasible test for improving BC detection and enabling pre-operative risk stratifications.

Methods: A urine-based DNA methylation assay was developed and validated by retrospective single-center studies in patients of suspected BC in Cohort 1 (n = 192) and Cohort 2 (n = 98), respectively. In addition, a prospective single-center study in hematuria patient group (Cohort 3, n = 174) was used as a second validation of the model.

Results: The assay with a dual-marker detection model showed 88.1% and 91.2% sensitivities, 89.7% and 85.7% specificities in validation Cohort 2 (patients of suspected BC) and Cohort 3 (patients of hematuria), respectively. Furthermore, this assay showed improved sensitivities over cytology and FISH on detecting low-grade tumor (66.7-77.8% vs. 0.0-22.2%, 0.0-22.2%), Ta tumor (83.3% vs. 22.2-41.2%, 44.4-52.9%) and non-muscle invasive BC (NMIBC) (80.0-89.7% vs. 51.5-52.0%, 59.4-72.0%) in both cohorts. The assay also had higher accuracies (88.9-95.8%) in diagnosing cases with concurrent genitourinary disorders as compared to cytology (55.6-70.8%) and FISH (72.2-77.8%). Meanwhile, the assay with a five-marker stratification model identified high-risk NMIBC and muscle invasive BC with 90.5% sensitivity and 86.8% specificity in Cohort 2.

Conclusions: The urine-based DNA methylation assay represents a highly sensitive and specific approach for BC early-stage detection and risk stratification. It has a potential to be used as a routine test to improve diagnosis and prognosis of BC in clinic.
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http://dx.doi.org/10.1186/s13148-021-01073-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072728PMC
April 2021

Effect of additives on properties and microstructure of lightweight aggregates produced from MSWI bottom ash sludge (MSWI-BAS).

J Air Waste Manag Assoc 2021 Apr 26. Epub 2021 Apr 26.

School of Environment, Nanjing Normal University, Nanjing, China.

In order to solve problems of land occupation and environment damage resulted from massive municipal solid waste incineration bottom ash sludge (MSWI-BAS), sintered lightweight aggregates (LWA) was prepared from MSWI-BAS. Additives are of great significance for the preparation of high-performance LWA and the utilization of MSWI-BAS resources, so their effect on properties of LWA was investigated. The results showed that when the content of water glass was 20%, compressive strength of LWA reached a maximum of 8.4 MPa, and 1h water absorption reached a minimum of 5%. The reason was the addition of water glass brought a lot of Na and Si(OH), the internal crystals of water glass were converted into rod-shaped zeolite crystals, thereby forming a high-density structure. The addition of coal powder led to the formation of gas in LWA, thus reducing the density of LWA. At the same time, it was also conducive to earlier generation of liquid phase in LWA, making its internal structure dense. When the content of coal powder was 5 %, 15 % and 20 %, the modification effect was better, and compressive strength of LWA was larger, about 4MPa. Additives are of great significance for the preparation of high-performance LWA and the utilization of MSWI-BAS resources. Implication StatementIn this study we have prepared LWA with MSWI-BAS. At the same time of XRD and FT-IR analysis of raw materials, we also investigated effect of water glass and coal powder on characteristics (particle density, 1h water absorption and compressive strength) of lightweight aggregates, and good results were obtained. For explanations, several characterizations were carried out, such as XRD, SEM and so on. The sludge disposal problem is reduced. It opens up a new way for the utilization of solid waste resources. In addition, it meets with the concept of green development of building materials, and makes the production of LWA have a broader development prospect.
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http://dx.doi.org/10.1080/10962247.2021.1918288DOI Listing
April 2021

Poor Stability of Li CO in the Solid Electrolyte Interphase of a Lithium-Metal Anode Revealed by Cryo-Electron Microscopy.

Adv Mater 2021 Apr 25:e2100404. Epub 2021 Apr 25.

Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.

The solid electrolyte interphase (SEI) dictates the cycling stability of lithium-metal batteries. Here, direct atomic imaging of the SEI's phase components and their spatial arrangement is achieved, using ultralow-dosage cryogenic transmission electron microscopy. The results show that, surprisingly, a lot of the deposited Li metal has amorphous atomic structure, likely due to carbon and oxygen impurities, and that crystalline lithium carbonate is not stable and readily decomposes when contacting the lithium metal. Lithium carbonate distributed in the outer SEI also continuously reacts with the electrolyte to produce gas, resulting in a dynamically evolving and porous SEI. Sulfur-containing additives cause the SEI to preferentially generate Li SO and overlithiated lithium sulfate and lithium oxide, which encapsulate lithium carbonate in the middle, limiting SEI thickening and enhancing battery life by a factor of ten. The spatial mapping of the SEI gradient amorphous (polymeric → inorganic → metallic) and crystalline phase components provides guidance for designing electrolyte additives.
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http://dx.doi.org/10.1002/adma.202100404DOI Listing
April 2021

Histone Lysine Methyltransferase SETD2 Regulates Coronary Vascular Development in Embryonic Mouse Hearts.

Front Cell Dev Biol 2021 9;9:651655. Epub 2021 Apr 9.

Department of Cardiovascular Surgery, Peking University Shenzhen Hospital, State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, China.

Congenital heart defects are the most common birth defect and have a clear genetic component, yet genomic structural variations or gene mutations account for only a third of the cases. Epigenomic dynamics during human heart organogenesis thus may play a critical role in regulating heart development. However, it is unclear how histone mark H3K36me3 acts on heart development. Here we report that histone-lysine N-methyltransferase SETD2, an H3K36me3 methyltransferase, is a crucial regulator of the mouse heart epigenome. is highly expressed in embryonic stages and accounts for a predominate role of H3K36me3 in the heart. Loss of in cardiac progenitors results in obvious coronary vascular defects and ventricular non-compaction, leading to fetus lethality in mid-gestation, without affecting peripheral blood vessel, yolk sac, and placenta formation. Furthermore, deletion of dramatically decreased H3K36me3 level and impacted the transcriptional landscape of key cardiac-related genes, including and . Taken together, our results strongly suggest that SETD2 plays a primary role in H3K36me3 and is critical for coronary vascular formation and heart development in mice.
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http://dx.doi.org/10.3389/fcell.2021.651655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063616PMC
April 2021

Intra-operative 3D hologram support with mixed reality technique based on CT-MRI fusion images: operation guidance for brain brachytherapy.

J Contemp Brachytherapy 2021 Apr 14;13(2):205-210. Epub 2021 Apr 14.

Interventional Center, the Affiliated Hospital of Qingdao University, Qingdao, China.

Purpose: The study investigated whether intra-operative 3D hologram, a computer graphics model of the brain, with mixed reality (MR) technique based on computed tomography-magnetic resonance imaging (CT-MRI) fusion images could be helpful during brachytherapy for brain metastasis.

Material And Methods: A patient with relapsed brain metastasis was reported. Pre-operative CT and MRI image fusion was performed, with software developed by our center and the Chinese University of Hong Kong. 3D polygon data segmented from pre-operative CT-MRI fusion images were registered into HoloLens (Microsoft Corporation, Redmond, WA, USA). I seed implantation was performed under MR guidance.

Results: Interventional surgeons could share the same hologram and move the hologram from their respective angles, while wearing the HoloLens without any monitors. The intra-operative hologram offered good visualization of the skull, tumor location, and main vessels around the tumor during brain brachytherapy of a patient with brain metastasis. The interventional surgeons could easily compare the patient's actual anatomy with the hologram before and during the brain brachytherapy procedure. D and V of post-operative plan and pre-operative plan were 131.8 Gy vs. 132.0 Gy and 94.8% vs. 94.0%, respectively. The patient's imaging findings together with clinical symptoms were greatly improved at 3 and 6 months after the implantation.

Conclusions: This initial experience suggests that the use of the intra-operative hologram with MR technique based on CT-MRI fusion images helped in brachytherapy guidance. Further research is needed to explore the potential of this approach in neuro-navigation.
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http://dx.doi.org/10.5114/jcb.2021.105289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060965PMC
April 2021