Publications by authors named "Hong Qin"

344 Publications

Impact of allograft types on outcomes after pediatric liver transplantation due to biliary atresia.

Pediatr Transplant 2022 Jun 23:e14342. Epub 2022 Jun 23.

Department of Pediatric transplantation, Organ Transplantation Center, Tianjin First Central Hospital, Tianjin, China.

Background: Several surgical strategies, including split donor transplantation and living donor transplantation, have been used to increase the donor liver pool. This report focuses on the effects of whole, split, and LDLT on recipient outcomes.

Methods: We retrospectively analyzed the records of all patients with biliary atresia at Tianjin First Central Hospital between April 2013 and December 2019.

Results: A total of 882 patients were included and divided into three groups by graft type, with 198 in the whole-liver-transplantation group, 78 in the split liver transplantation group, and 606 in the LDLT group. The median follow-up time was 39 months, patient survival rates of three groups were 94.4%, 88.5%, and 95.0%, respectively, and graft survival rates were 90.2%, 83.3%, and 94.7%, respectively. We divided the split liver transplantation group into two subgroups according to the donor's age, and patient survival rates exhibited a significant difference only in the group whose donor age was over 45 years. The postoperative complication rates were significantly higher with respect to hepatic artery thrombosis, portal stenosis, and AR; and lower in hepatic venous stenosis, PTLDs, CMV virus, and EBV infection in the WLT group. Our multivariate model showed that donor age ≥45 years, RBC transfusion, pneumonia, and HAT were the independent predictors of allograft loss.

Conclusions: The survival of split liver transplantation group was slightly lower. The types of complications are different from different graft types. Therefore, postoperative monitoring and treatment need to be adjusted according to the different graft types used.
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http://dx.doi.org/10.1111/petr.14342DOI Listing
June 2022

Outcome of Split Liver Transplantation from Pediatric Donors Weighing 25 kg or Less.

Liver Transpl 2022 Jun 17. Epub 2022 Jun 17.

Organ Transplantation Center, Tianjin, Tianjin, China.

The lower limit of body weight for "splitable" liver grafts remains unknown. To examine the outcome of split liver transplantation (SLT) from pediatric donors ≤ 25 kg relative to conventional graft type liver transplantation from deceased donors under corresponding conditions, a total of 158 patients who received primary liver transplantation, including 22 SLTs from donors ≤ 25 kg, 46 SLTs from donors > 25 kg, 76 whole liver transplantations (WLTs) and 14 reduced liver transplantations (RLTs) in donors ≤ 25 kg, between January 2018 and December 2019 were included in the study. There was no significant difference in the complications, patient survival and graft survival between each of the latter three groups and SLT ≤ 25 kg group. Pediatric end-stage liver disease (PELD) score was the independent predictor of graft loss (death or retransplantation). Graft weight was the independent predictor of hepatic artery thrombosis (HAT). SLT using well-selected pediatric donors ≤ 25 kg is an effective strategy to increase organ availability, especially for low body weight recipients, compared with conventional graft type from deceased donors under the condition of corresponding donor weight without increasing morbidity and mortality.
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http://dx.doi.org/10.1002/lt.26530DOI Listing
June 2022

Management and outcome of hepatic artery thrombosis with whole-liver transplantation using donors less than one year of age.

J Pediatr Surg 2022 May 16. Epub 2022 May 16.

Department of Pediatric transplantation, Organ Transplantation Center, Tianjin First Central Hospital, No. 24 Fukang Road, Nakao District, Tianjin 300192, PR China; Tianjin Key Laboratory of Organ Transplantation, Tianjin, PR China. Electronic address:

Background/purpose: The incidence of hepatic artery thrombosis (HAT) in recipients is high after pediatric LT using young donors. In this study we investigated the management and outcome of HAT after whole-LT using donors less than one year of age. And evaluate the safety of pediatric donors, and increase the utilization of pediatric donors overall.

Methods: We retrospectively analyzed the clinical data encompassing children who underwent whole-liver transplantation in our department from January 2014 to December 2019. Recipients receiving a liver from a donor ≥1 month and ≤12 months were included, and a total of 110 patients were included in this study.

Results: The results showed an incidence for HAT of 20% and the median time to HAT diagnosis was 3.0 (2.0, 5.3) days post-operation. Anticoagulant therapy was used for 19 cases and 94.7% of them achieved hepatic artery recanalization or collateral formation. The median time of recanalization was 12 (5, 15) days. Bile leakage and biliary strictures occurring in the HAT group were higher than in the non HAT group (13.6% vs. 1.1% and 31.8% vs. 3.4%). There were no significant differences in the survival rates of recipients or grafts among the two groups (P = 0.474, P = 0.208, respectively).

Conclusion: We confirmed that the incidence of HAT in LT recipients use donors less than 1 year is high, but recanalization can be performed using anticoagulant therapy. Although biliary complications increased significantly after HAT, the survival rates of patients and grafts were satisfactory.

Level Of Evidence: Level III.
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http://dx.doi.org/10.1016/j.jpedsurg.2022.05.009DOI Listing
May 2022

The development, validity, reliability, and norm of a preschool auditory processing assessment scale in China.

Res Dev Disabil 2022 Jun 4;128:104272. Epub 2022 Jun 4.

The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Jiangsu, China. Electronic address:

Background: Children with auditory processing deficits may face problems with language, learning, and social communication.

Aims: To develop a Chinese auditory processing assessment scale for preschool children and establish the norms of the scale.

Methods And Procedures: The predictive version of the scale was formed by a literature review, qualitative interviews, expert consultation, and a pre-test with a small sample. Nine kindergartens in Nanjing were selected by a stratified cluster sampling plan. First, 734 children from two kindergartens were selected for the large sample pre-test of the scale. Then, 1526 children from four kindergartens and 1151 children from three kindergartens were selected for the reliability and validity analysis and confirmatory factor analysis, respectively. The standardized norm data of the scale were established based on the 3411 points of scale data of the nine kindergartens. Finally, the clinical usefulness of the scale was analyzed by comparing the results of objective auditory processing tests in children with normal and abnormal auditory processing prompted by the score on the scale.

Outcomes And Results: The preschool auditory processing assessment scale includes 5 dimensions and 30 items. The Cronbach's alpha value of the scale is greater than 0.9. The confirmatory factor analysis results verify that the scale structure is reasonable. The percentile norm of the scale was established. The results of electrophysiological tests of the normal and abnormal auditory processing groups were statistically different (P < 0.05).

Conclusions And Implications: The developed preschool auditory processing assessment scale has good reliability and validity. The scale is suitable for clinical application.
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http://dx.doi.org/10.1016/j.ridd.2022.104272DOI Listing
June 2022

Structural and Temporal Dynamics of Mesenchymal Stem Cells in Liver Diseases From 2001 to 2021: A Bibliometric Analysis.

Front Immunol 2022 19;13:859972. Epub 2022 May 19.

Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China.

Background: Mesenchymal stem cells (MSCs) have important research value and broad application prospects in liver diseases. This study aims to comprehensively review the cooperation and influence of countries, institutions, authors, and journals in the field of MSCs in liver diseases from the perspective of bibliometrics, evaluate the clustering evolution of knowledge structure, and discover hot trends and emerging topics.

Methods: The articles and reviews related to MSCs in liver diseases were retrieved from the Web of Science Core Collection using Topic Search. A bibliometric study was performed using CiteSpace and VOSviewer.

Results: A total of 3404 articles and reviews were included over the period 2001-2021. The number of articles regarding MSCs in liver diseases showed an increasing trend. These publications mainly come from 3251 institutions in 113 countries led by China and the USA. Li L published the most papers among the publications, while Pittenger MF had the most co-citations. Analysis of the most productive journals shows that most are specialized in medical research, experimental medicine and cell biology, and cell & tissue engineering. The macroscopical sketch and micro-representation of the whole knowledge field are realized through co-citation analysis. Liver scaffold, MSC therapy, extracellular vesicle, and others are current and developing areas of the study. The keywords "machine perfusion", "liver transplantation", and "microRNAs" also may be the focus of new trends and future research.

Conclusions: In this study, bibliometrics and visual methods were used to review the research of MSCs in liver diseases comprehensively. This paper will help scholars better understand the dynamic evolution of the application of MSCs in liver diseases and point out the direction for future research.
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http://dx.doi.org/10.3389/fimmu.2022.859972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160197PMC
June 2022

Clinical parameters combined with radiomics features of PET/CT can predict recurrence in patients with high-risk pediatric neuroblastoma.

BMC Med Imaging 2022 May 28;22(1):102. Epub 2022 May 28.

Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, 95 Yong An Road, Xi Cheng District, Beijing, 100045, China.

Background: This retrospective study aimed to develop and validate a combined model based [F]FDG PET/CT radiomics and clinical parameters for predicting recurrence in high-risk pediatric neuroblastoma patients.

Methods: Eighty-four high-risk neuroblastoma patients were retrospectively enrolled and divided into training and test sets according to the ratio of 3:2. [F]FDG PET/CT images of the tumor were segmented by 3D Slicer software and the radiomics features were extracted. The effective features were selected by the least absolute shrinkage and selection operator to construct the radiomics score (Rad_score). And the radiomics model (R_model) was constructed based on Rad_score for prediction of recurrence. Then, univariate and multivariate analyses were used to screen out the independent clinical risk parameters and construct the clinical model (C_model). A combined model (RC_model) was developed based on the Rad_score and independent clinical risk parameters and presented as radiomics nomogram. The performance of the above three models was assessed by the area under the receiver operating characteristic curve (AUC) and decision curve analysis (DCA).

Results: Seven radiomics features were selected for building the R_model. The AUCs of the C_model in training and test sets were 0.744 (95% confidence interval [CI], 0.595-0.874) and 0.750 (95% CI, 0.577-0.904), respectively. The R_model yielded AUCs of 0.813 (95% CI, 0.685-0.916) and 0.869 (95% CI, 0.715-0.985) in the training and test sets, respectively. The RC_model demonstrated the largest AUCs of 0.889 (95% CI, 0.794-0.963) and 0.892 (95% CI, 0.758-0.992) in the training and test sets, respectively. DCA demonstrated that RC_model added more net benefits than either the C_model or the R_model for predicting recurrence in high-risk pediatric neuroblastoma.

Conclusions: The combined model performed well for predicting recurrence in high-risk pediatric neuroblastoma, which can facilitate disease follow-up and management in clinical practice.
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http://dx.doi.org/10.1186/s12880-022-00828-zDOI Listing
May 2022

Technology investigation on time series classification and prediction.

PeerJ Comput Sci 2022 18;8:e982. Epub 2022 May 18.

National Engineering Laboratory for Agri-product Quality Traceability, Beijing Technology and Business University, Beijing, China.

Time series appear in many scientific fields and are an important type of data. The use of time series analysis techniques is an essential means of discovering the knowledge hidden in this type of data. In recent years, many scholars have achieved fruitful results in the study of time series. A statistical analysis of 120,000 literatures published between 2017 and 2021 reveals that the topical research about time series is mostly focused on their classification and prediction. Therefore, in this study, we focus on analyzing the technical development routes of time series classification and prediction algorithms. 87 literatures with high relevance and high citation are selected for analysis, aiming to provide a more comprehensive reference base for interested researchers. For time series classification, it is divided into supervised methods, semi-supervised methods, and early classification of time series, which are key extensions of time series classification tasks. For time series prediction, from classical statistical methods, to neural network methods, and then to fuzzy modeling and transfer learning methods, the performance and applications of these different methods are discussed. We hope this article can help aid the understanding of the current development status and discover possible future research directions, such as exploring interpretability of time series analysis and online learning modeling.
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http://dx.doi.org/10.7717/peerj-cs.982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138170PMC
May 2022

Development and Validation of a Novel Diagnostic Model for Childhood Autism Spectrum Disorder Based on Ferroptosis-Related Genes.

Front Psychiatry 2022 12;13:886055. Epub 2022 May 12.

Department of Child Health Care, Nanjing Maternity and Child Health Care Hospital, Women's Hospital of Nanjing Medical University, Nanjing, China.

Autism spectrum disorder (ASD) is a highly genetic heterogeneous neurodevelopmental disorder, which is usually considered a heritable and heterogeneous neurodevelopmental disorder and has caused a great burden to society and families. Emerging roles of ferroptosis have been observed in neurological disorders. This study aimed to construct a diagnostic model based on ferroptosis-related genes (FRGs) to contribute to the early and precise diagnosis of childhood ASD. In the candidate FRGs, we identified 27 differentially expressed genes (DEGs) between ASD patients and typically developing (TD) controls. Four key FRGs were identified using the random forest analysis for further analysis. Utilization of the four gene expression, we constructed a diagnostic model and the AUC value in the training dataset (GSE18123) is 0.7002. We deem that a patient with a score less than 0.9904 is likely to have ASD. Three validation datasets (GSE111176, GSE113834, and GSE28521) were collected and the AUC value is 0.7442, 0.7444, and 0.6474, respectively. A multi-factor regulatory network based on four FRGs indicated that RORA, EAF1, NFYB, miR-4703-3p, and miR-6073 may play a role in the development of ASD. In addition, we found piperaquine may have the potential to be a promising drug for the treatment of ASD. Overall, we constructed a diagnostic model of childhood ASD, which could contribute to the precision diagnosis and timely treatment of childhood ASD.
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http://dx.doi.org/10.3389/fpsyt.2022.886055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133509PMC
May 2022

Facile synthesis of cadmium-doped graphite carbon nitride for photocatalytic degradation of tetracycline under visible light irradiation.

Environ Sci Pollut Res Int 2022 May 28. Epub 2022 May 28.

College of Environmental Science and Engineering, Hunan University, Lushan South Road, Yuelu District, Changsha, 410082, People's Republic of China.

In recent years, using semiconductor photocatalysts for antibiotic contaminant degradation under visible light has become a hot topic. Herein, a novel and ingenious cadmium-doped graphite phase carbon nitride (Cd-g-CN) photocatalyst was successfully constructed via the thermal polymerization method. Experimental and characterization results revealed that cadmium (Cd) was well doped at the g-CN surface and exhibited high intercontact with g-CN. Additionally, the introduction of cadmium significantly improved the photocatalytic activity, and the optimum degradation efficiency of tetracycline (TC) reached 98.1%, which was exceeded 2.0 times that of g-CN (43.9%). Meanwhile, the Cd-doped sample presented a higher efficiency of electrical conductivity, light absorption property, and photogenerated electron-hole pair migration compared with g-CN. Additionally, the quenching experiments and electron spin-resonance tests exhibited that holes (h), hydroxyl radicals (•OH), superoxide radicals (•O) were the main active species involved in TC degradation. The effects of various conditions on photocatalytic degradation, such as pH, initial TC concentrations, and catalyst dosage, were also researched. Finally, the degradation mechanism was elaborated in detail. This work gives a reasonable point to synthesizing high-efficiency and economic metal-doped photocatalysts.
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http://dx.doi.org/10.1007/s11356-022-21051-xDOI Listing
May 2022

Scene-graph-driven semantic feature matching for monocular digestive endoscopy.

Comput Biol Med 2022 Jul 16;146:105616. Epub 2022 May 16.

Department of Computer Science, Stony Brook University, New York, USA.

Background And Objective: Registration of the preoperative 3D model with the video of the digestive tract is the key task in endoscopy surgical navigation. Accurate 3D reconstruction of soft tissue surfaces is essential to complete registration. However, existing feature matching methods still fall short of desirable performance, due to the soft tissue deformation and smooth but less-textured surface.

Methods: In this paper, we present a new semantic description based on the scene graph to integrate contour features and SIFT features. Firstly, we construct the semantic feature descriptor using the SIFT features and dense points in the contour regions to obtain more dense point feature matching. Secondly, we design a clustering algorithm based on the proposed semantic feature descriptor. Finally, we apply the semantic description to the structure from motion (SfM) reconstruction framework.

Results: Our techniques are validated by the phantom tests and real surgery videos. We compare our approaches with other typical methods in contour extraction, feature matching, and SfM reconstruction. On average, the feature matching accuracy reaches 75.6% and improves 16.6% in pose estimation. In addition, 39.8% of sparse points are increased in SfM results, and 35.31% more valid points are obtained for the DenseDescriptorNet training in 3D reconstruction.

Conclusions: The new semantic feature description has the potential to reveal more accurate and dense feature correspondence and provides local semantic information in feature matching. Our experiments on the clinical dataset demonstrate the effectiveness and robustness of the novel approach.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105616DOI Listing
July 2022

Vitamin A supplementation ameliorates prenatal valproic acid-induced autism-like behaviors in rats.

Neurotoxicology 2022 May 17;91:155-165. Epub 2022 May 17.

Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, 123 Tianfei Alley, Mochou Road, Nanjing 210004, PR China; Institute of Pediatrics, Nanjing Medical University, 72 Guangzhou Road, Nanjing 210008, PR China. Electronic address:

Introduction: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by social deficits and repetitive stereotyped behaviors. Prenatal exposure to the anticonvulsant drug valproic acid (VPA) is reported to induce ASD in human and ASD-like phenotypes in rodents. Unfortunately, the etiology and pathogenesis of ASD remains unclear.

Methods: Pregnant rats received an intraperitoneal injection of 600 mg/kg VPA on E12.5 to construct the ASD rat model in offspring. The different expression of long non-coding RNA (lncRNA) and mRNA profiles in the hippocampus were determined by RNA sequencing to investigate potential mechanisms of VPA-induced ASD. Gene Ontology (GO) and pathway enrichment analysis were performed to predict the function of dysregulated lncRNAs. Co-expression network and real-time polymerase chain reaction (RT-PCR) analysis were conducted to validate the potential regulatory lncRNA-mRNA network.

Results: VPA increased the total distance, time spent in the central zone and self-grooming (open field test) in rats. Meanwhile, VPA induced social impairment (three-chamber sociability test) and repetitive behaviors (marble burying test). A total of 238 lncRNAs and 354 mRNAs were differentially expressed in the VPA group. In addition, the dysregulated lncRNAs were involved in neural function and developmental processes of ASD. 5 lncRNAs and 7 mRNAs were differently expressed and included in the lncRNA-mRNA co-expression network. RT-PCR confirmed the upregulation of 4 lncRNAs and 6 mRNAs, and identified a potential regulatory network of NONRATT021475.2 (lncRNA) and Desert hedgehog (Dhh). Moreover, VPA decreased the serum vitamin A (VA) levels in offspring rats on postnatal day (PND) 21 and 49. Importantly, VA supplementation significantly restored VPA-induced autism-related behaviors and upregulation of NONRATT021475.2 and Dhh in the hippocampus of ASD rats.

Conclusion: This study not only contributed to understand the importance of lncRNAs and mRNAs in the progression of ASD, but also identified VA as a potential therapy for the condition.

Data Availability: The data that support the findings of this study are available from the corresponding author with reasonable request.
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http://dx.doi.org/10.1016/j.neuro.2022.05.008DOI Listing
May 2022

Estimation and Identification of Nonlinear Parameter of Motion Index Based on Least Squares Algorithm.

Authors:
Hong Qin

Comput Intell Neurosci 2022 2;2022:7383074. Epub 2022 May 2.

Department of Physical Education, Zhongyuan University of Technology, Zhengzhou, Henan 450007, China.

Parameter identification is an important branch of automatic control. Due to its special function, it has been widely used in various fields, especially the modeling of complex systems or systems whose parameters are not easy to determine. With the development of control technology, the scale of the control object is getting larger and larger, which makes the calculation amount of the identification algorithm larger and larger. For the nonlinear system with complex structure, especially the nonlinear system containing the product of unknown parameters, the number of parameters of the over-parameterized identification method increases greatly, and the calculation amount of the identification algorithm also increases sharply. Therefore, a parameter estimation method with a small amount of calculation is explored. The results show that the proposed method can overcome the phenomenon of "data saturation", thus improving the parameter identification results.
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http://dx.doi.org/10.1155/2022/7383074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085361PMC
May 2022

Cobalt Single Atoms Anchored on Oxygen-Doped Tubular Carbon Nitride for Efficient Peroxymonosulfate Activation: Simultaneous Coordination Structure and Morphology Modulation.

Angew Chem Int Ed Engl 2022 May 5:e202202338. Epub 2022 May 5.

College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education), Hunan University, Changsha, 410082, P. R. China.

Simultaneous regulation of the coordination environment of single-atom catalysts (SACs) and engineering architectures with efficient exposed active sites are efficient strategies for boosting peroxymonosulfate (PMS) activation. We isolated cobalt atoms with dual nitrogen and oxygen coordination (Co-N O ) on oxygen-doped tubular carbon nitride (TCN) by pyrolyzing a hydrogen-bonded cyanuric acid melamine-cobalt acetate precursor. The theoretically constructed Co-N O moiety on TCN exhibited an impressive mass activity of 7.61×10  min  mol with high O selectivity. Theoretical calculations revealed that the cobalt single atoms occupied a dual nitrogen and oxygen coordination environment, and that PMS adsorption was promoted and energy barriers reduced for the key *O intermediate that produced O . The catalysts were attached to a widely used poly(vinylidene fluoride) microfiltration membrane to deliver an antibiotic wastewater treatment system with 97.5 % ciprofloxacin rejection over 10 hours, thereby revealing the suitability of the membrane for industrial applications.
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http://dx.doi.org/10.1002/anie.202202338DOI Listing
May 2022

Metal-organic frameworks as a good platform for the fabrication of multi-metal nanomaterials: design strategies, electrocatalytic applications and prospective.

Adv Colloid Interface Sci 2022 Jun 10;304:102668. Epub 2022 Apr 10.

College of Environmental Science and Engineering and Key Laboratory of Environmental Biology and Pollution Control (Ministry of Education), Hunan University, Changsha 410082, PR China; Department of Urology, Second Xiangya Hospital, Central South University, Changsha 410011, PR China. Electronic address:

MOF-derived multi-metal nanomaterials are attracting numerous attentions in widespread applications such as catalysis, sensors, energy storage and conversion, and environmental remediation. Compared to the monometallic counterparts, the presence of foreign metal is expected to bring new physicochemical properties, thus exhibiting synergistic effect for enhanced performance. MOFs have been proved as a good platform for the fabrication of polymetallic nanomaterials with requisite features. Herein, various design strategies related to constructing multi-metallic nanomaterials from MOFs are summarized for the first time, involving metal nodal substitution, seed epitaxial growth, ion-exchange strategy, guest species encapsulation, solution impregnation and combination with extraneous substrate. Afterwards, the recent advances of multi-metallic nanomaterials for electrocatalytic applications, including oxygen reduction reaction (ORR), oxygen evolution reaction (OER) and hydrogen evolution reaction (HER), are systematically discussed. Finally, a personal outlook on the future trends and challenges are also presented with hope to enlighten deeper understanding and new thoughts for the development of multi-metal nanomaterials from MOFs.
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http://dx.doi.org/10.1016/j.cis.2022.102668DOI Listing
June 2022

Establishing Normative Data for the Number Cancelation Test Among Children in Kindergartens and Primary Schools in China.

Front Psychiatry 2022 11;13:788825. Epub 2022 Apr 11.

Key Laboratory of Child Development and Learning Science of Ministry of Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.

This study aimed to suggest an attention assessment tool using a Digital Pen for measuring the temporal-spatial parameters during the Number Cancelation Test (NCT), and then to establish the normative data for the NCT among children in kindergartens and primary schools in China by recruiting a total of 989 children (496 males). Four measures, i.e., selective attention (SA), speed of cognitive processing (SpC), averaged time of circlings (ATC), and averaged circumference of circled curves (ACCC), were proposed to evaluate the NCT performance. They basically have a development trend with fast speed in the beginning before Grade 1 or 2 of primary schools, and then enter an extremely slow development period (with ceiling or floor effect). SA and SpC have gender and grade main effects, while ATC and ACCC have the grade main effect, only. In particular, females have higher SA scores than males in middle class of kindergarten, and Grade 2-Grade 5 of primary school, but no gender differences in other grades; females have higher SpC scores than males in middle class of kindergarten, and Grade 3-4 of primary school, but no gender differences in other grades. More importantly, in clinical practice, if SA or SpC measure of a child is below than the 5th centile (i.e., p5 level) of his/her grade-specific normative data, then this child may be predicted to have a high-risk of learning disabilities. Findings suggest that the proposed method can be used for early screening of learning disabilities by setting appropriate cut-off values.
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http://dx.doi.org/10.3389/fpsyt.2022.788825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035546PMC
April 2022

Noninvasive isolation of transcervical trophoblast cells for fetal identification.

J Obstet Gynaecol Res 2022 Apr 22. Epub 2022 Apr 22.

Obstetrics and Gynecology Department, the 8th People's Hospital of Shanghai, Shanghai, China.

Objective: To identify trophoblastic cells retrieved from the cervix at a gestational age (GA) of 5-9 weeks by a noninvasive modality in fetuses.

Method: Transcervical cells (TCCs) were noninvasively extracted by a cytobrush using the Papanicolaou sampling method. TCCs were immunostained with antihuman leukocyte antigen (HLA)-G and anticytokeratin (CK)-7 antibodies to identify trophoblastic cells. Maternal finger blood, gestational sacs, and 20 trophoblastic cells collected by a laser-guided microscopic single-cell capture system were examined and compared by short tandem repeat (STR) genotyping.

Results: Forty-nine pregnant women with GA of 5-9 weeks and six nonpregnant healthy women were included in the study. Trophoblastic cells were identified in 37 (75.5%) TCC samples, among which 34 (69.4%) were eligible for STR genotyping analysis. No trophoblastic cells were identified in nonpregnant healthy women. The STR genotyping analyses revealed 24 female and 10 male fetuses. TCC trophoblastic cells exhibited the same STR profiles as gestational sac and maternal blood in all samples, which indicated that the TCC trophoblastic cells originated from fetuses.

Conclusion: This primary study validated that trophoblastic cells from TCCs at GA 5-9 weeks originated from the fetus. Further studies are needed to verify whether this method can be used for early noninvasive prenatal diagnosis and paternity testing.
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http://dx.doi.org/10.1111/jog.15256DOI Listing
April 2022

N6-Methyladenine-Related Signature for Immune Microenvironment and Response to Immunotherapy in Hepatocellular Carcinoma.

Int J Gen Med 2022 30;15:3525-3540. Epub 2022 Mar 30.

Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China.

Background: The prognostic value of m6A-related genes in hepatocellular carcinoma (HCC) and its correlation with the immune microenvironment still requires further investigation.

Methods: Consensus clustering by m6A related genes was used to classify 374 patients with HCC from The Cancer Genome Atlas (TCGA) database. Then we performed the least absolute shrinkage and selection operator (LASSO) to construct the m6A related genes model. The International Cancer Genome Consortium (ICGC) and Gene Expression Omnibus (GEO) datasets were used to verify and evaluate the model. ESTIMATE, CIBERSORTx, the expression levels of immune checkpoint genes, and TIDE were used to investigate the tumor microenvironment (TME) and the response to immunotherapy. Gene set enrichment analyses (GSEA), tumor-associated macrophages (TAMs), and gene-drug sensitivity were also analyzed.

Results: By expression value and regression coefficient of five m6A related genes, we constructed the risk score of each patient. The patients with a higher risk score had a considerably poorer prognosis in the primary and validated cohort. For further discussing TME and the response to immunotherapy, we divided the entire set into two groups based on the risk score. Our findings implied that the tumor-infiltrating lymphocytes (TILs) were proportional to the risk scores, which seemed to contradict that patients with higher scores had a poor prognosis. Further, we found that the high-risk group had higher expression of PD-L1, CTLA-4, and PDCD1, indicating immune dysfunction, which may be a fundamental reason for poor prognosis. This was further reinforced by the fact that the low-risk group responded better than the high-risk group to monotherapy and combination therapy.

Conclusion: The m6A related risk score is a new independent prognostic factor that correlates with immunotherapy response. It can provide a new therapeutic strategy for improving individual immunotherapy in HCC.
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http://dx.doi.org/10.2147/IJGM.S351815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978579PMC
March 2022

Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism.

Food Nutr Res 2022 17;66. Epub 2022 Mar 17.

Department of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, Changsha, China.

Background: Globally, obesity is a significant public problem, especially when aging. Sesamol, a phenolic lignan present in sesame seeds, might have a positive effect on high-fat diet (HFD)-induced obesity associated with aging.

Objective: The purpose of current research study was to explore salutary effects and mechanisms of sesamol in treating alimentary obesity and associated metabolic syndrome in middle-aged mice.

Methods: C57BL/6J mice aged 4-6 weeks and 6-8 months were assigned to the young normal diet group, middle-aged normal diet group, middle-aged HFD group, and middle-aged HFD + sesamol group. At the end of experiment, glucose tolerance test and insulin tolerance test were performed; the levels of lipids and oxidative stress-related factors in the serum and skeletal muscle were detected using chemistry reagent kits; lipid accumulation in skeletal muscle was observed by oil red O staining; the expressions of muscular glucose and lipid metabolism associated proteins were measured by Western blotting.

Results: Sesamol decreased the body weight and alleviated obesity-associated metabolism syndrome in middle-aged mice, such as glucose intolerance, insulin resistance, dyslipidemia, and oxidative stress. Moreover, muscular metabolic disorders were attenuated after treatment with sesamol. It increased the expression of glucose transporter type-4 and down-regulated the protein levels of pyruvate dehydrogenase kinase isozyme 4, implying the increase of glucose uptake and oxidation. Meanwhile, sesamol decreased the expression of sterol regulatory element binding protein 1c and up-regulated the phosphorylation of hormone-sensitive lipase and the level of carnitine palmityl transferase 1α, which led to the declined lipogenesis and the increased lipolysis and lipid oxidation. In addition, the SIRT1/AMPK signaling pathway was triggered by sesamol, from which it is understood how sesamol enhances glucose and lipid metabolism.

Conclusions: Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism, which might be associated with the stimulation of the SIRT1/AMPK pathway.
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http://dx.doi.org/10.29219/fnr.v66.8231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941404PMC
March 2022

Prediction of MYCN Amplification, 1p and 11q Aberrations in Pediatric Neuroblastoma Pre-therapy 18F-FDG PET/CT Radiomics.

Front Med (Lausanne) 2022 18;9:840777. Epub 2022 Mar 18.

Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Purpose: This study aimed to assess the predictive ability of 18F-FDG PET/CT radiomic features for MYCN, 1p and 11q abnormalities in NB.

Method: One hundred and twenty-two pediatric patients (median age 3. 2 years, range, 0.2-9.8 years) with NB were retrospectively enrolled. Significant features by multivariable logistic regression were retained to establish a clinical model (C_model), which included clinical characteristics. 18F-FDG PET/CT radiomic features were extracted by Computational Environment for Radiological Research. The least absolute shrinkage and selection operator (LASSO) regression was used to select radiomic features and build models (R-model). The predictive performance of models constructed by clinical characteristic (C_model), radiomic signature (R_model), and their combinations (CR_model) were compared using receiver operating curves (ROCs). Nomograms based on the radiomic score (rad-score) and clinical parameters were developed.

Results: The patients were classified into a training set ( = 86) and a test set ( = 36). Accordingly, 6, 8, and 7 radiomic features were selected to establish R_models for predicting MYCN, 1p and 11q status. The R_models showed a strong power for identifying these aberrations, with area under ROC curves (AUCs) of 0.96, 0.89, and 0.89 in the training set and 0.92, 0.85, and 0.84 in the test set. When combining clinical characteristics and radiomic signature, the AUCs increased to 0.98, 0.91, and 0.93 in the training set and 0.96, 0.88, and 0.89 in the test set. The CR_models had the greatest performance for MYCN, 1p and 11q predictions ( < 0.05).

Conclusions: The pre-therapy 18F-FDG PET/CT radiomics is able to predict MYCN amplification and 1p and 11 aberrations in pediatric NB, thus aiding tumor stage, risk stratification and disease management in the clinical practice.
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http://dx.doi.org/10.3389/fmed.2022.840777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971895PMC
March 2022

Research on the Evaluation Method of Enterprises' Independent Innovation Ability Based on Improved BP Neural Network and DQN Algorithm.

Comput Intell Neurosci 2022 24;2022:8250879. Epub 2022 Mar 24.

College of Management, Ocean University of China, Qingdao, Shandong 266100, China.

The development of enterprises has a very important influence on promoting national economic growth and improving comprehensive economic strength. This work evaluates the independent innovation ability of enterprises, analyzes the characteristics and difficulties of technological innovation of enterprises, and proposes corresponding solutions to promote independent technological innovation of enterprises. Firstly, the characteristics of the research object are clarified, and on the basis of relevant research, the theory of technological innovation and evaluation at home and abroad is expounded. At the same time, the basic theory of the improved BP neural network and DQN algorithm is introduced, which provides a theoretical basis for the research of the thesis. Secondly, according to the characteristics of enterprise technological innovation, an index system for evaluating the technological innovation capability of enterprises is constructed. Then, according to the related theory of the improved BP neural network and DQN algorithm, a neural network model for evaluating the technological innovation capability of enterprises is designed, and the validity of the model is verified through empirical research. Finally, this paper applies the evaluation model to the surveyed enterprises, comprehensively analyzes the characteristics and existing problems of independent technological innovation of enterprises, and proposes practical and feasible countermeasures to improve technological innovation capabilities from the perspective of enterprises themselves. The research results of this paper can be used as an effective supplement to the research on independent technological innovation of enterprises, and at the same time promote the continuous improvement of independent technological innovation capabilities of enterprises.
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http://dx.doi.org/10.1155/2022/8250879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8970940PMC
April 2022

Corrigendum to "The role of KLF4 in melanogenesis and homeostasis in sheep melanocytes" [Acta Histochem. 124 (2022) 151839].

Acta Histochem 2022 May 25;124(4):151889. Epub 2022 Mar 25.

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, No. 1 Mingxian South Road, Taigu 030801, China. Electronic address:

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http://dx.doi.org/10.1016/j.acthis.2022.151889DOI Listing
May 2022

Liquiritigenin Inhibits Lipid Accumulation in 3T3-L1 Cells via mTOR-Mediated Regulation of the Autophagy Mechanism.

Nutrients 2022 Mar 18;14(6). Epub 2022 Mar 18.

Department of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, China.

Liquiritigenin (LQG) is a natural flavonoid from the herb Glycyrrhiza uralensis Fisch that exhibits multiple biological activities. However, its specific role in antiobesity and its related underlying molecular mechanisms remain unknown. The primary purpose of this study is to explore the effects and regulatory mechanisms of LQG on lipid accumulation in 3T3-L1 adipocytes. The results show that LQG significantly reduced triglyceride levels and downregulated the expression of transcription factors such as CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ) in 3T3-L1 adipocytes. Additionally, the expression of sterol-regulatory element-binding protein 1c (SREBP1c), acetyl-CoA carboxylase 1 (ACC1), and fatty acid synthase (FASN) involved in lipogenesis was reduced by treatment with LQG. The protein expression levels of light chain 3B (LC3B), autophagy-related protein 7 (ATG7) and p62 were also modulated by LQG, leading to the suppression of autophagy. Further, LQG activated the phosphorylation of the mammalian target of rapamycin (mTOR), the inhibition of which was followed by the restored expression of autophagy-related proteins. Pretreatment with an mTOR inhibitor also reverted the expression of several genes or proteins involved in lipid synthesis. These results suggest that LQG inhibited lipid accumulation via mTOR-mediated autophagy in 3T3-L1 white adipocytes, indicating the role of LQG as a potential natural bioactive component for use in dietary supplements for preventing obesity.
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http://dx.doi.org/10.3390/nu14061287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954126PMC
March 2022

The single nucleotide polymorphism rs1814521 in long non-coding RNA ADGRG3 associates with the susceptibility to silicosis: a multi-stage study.

Environ Health Prev Med 2022 ;27(0)

Department of Occupational Health, Center for Disease Control and Prevention of Wuxi.

Background: This study aimed to evaluate the correlation between long non-coding RNA (lncRNA)-related single nucleotide polymorphisms (SNPs) and susceptibility to silicosis.

Methods: First, RNA-sequencing (RNA-seq) data were comprehensively analyzed in the peripheral blood lymphocytes of eight participants (four silicosis cases and four healthy controls) exposed to silica dust to identify differentially expressed lncRNAs (DE-lncRNAs). The functional SNPs in the identified DE-lncRNAs were then identified using several databases. Finally, the association between functional SNPs and susceptibility to silicosis was evaluated by a two-stage case-control study. The SNPs of 155 silicosis cases and 141 healthy silica-exposed controls were screened by genome-wide association study (GWAS), and the candidate SNPs of 194 silicosis cases and 235 healthy silica-exposed controls were validated by genotyping using the improved Mutiligase Detection Reaction (iMLDR) system.

Results: A total of 76 DE-lncRNAs were identified by RNA-seq data analysis (cut-offs: fold change > 2 or fold change < 0.5, P < 0.05), while 127 functional SNPs among those 76 DE-lncRNAs were identified through multiple public databases. Furthermore, five SNPs were found to be significantly correlated with the risk of silicosis by GWAS screening (P < 0.05), while the results of GWAS and iMLDR validation indicated that the variant A allele of rs1814521 was associated with a reduced risk of silicosis (OR = 0.76, 95% CI = 0.62-0.94, P = 0.011).

Conclusion: The presence of the SNP rs1814521 in the lncRNA ADGRG3 is associated with susceptibility to silicosis. Moreover, ADGRG3 was found to be lowly expressed in silicosis cases. The underlying biological mechanisms by which lncRNA ADGRG3 and rs1814521 regulate the development of silicosis need further study.
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http://dx.doi.org/10.1265/ehpm.21-00338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9093617PMC
March 2022

Mechanism of Alleviating Acquired Hyperlipidemia Induced by High-Fat Diet through Regulating Lipid Metabolism.

Nutrients 2022 Feb 23;14(5). Epub 2022 Feb 23.

Department of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, China.

(AM) is a food and medicinal homologous plant. The current research is aimed to investigate the beneficial effects and mechanisms of AM in treating acquired hyperlipidemia. The network pharmacology and bioinformatics analysis results showed 481 AM-related targets and 474 acquired hyperlipidemia-associated targets, and 101 candidate targets were obtained through the intersection, mainly enriched in endocrine resistance, AGE-RAGE in diabetic complications and p53 signaling pathways. , , , were determined as the candidate active components of AM in the treatment of acquired hyperlipidemia. Moreover, key targets of AM, namely, AKT serine/threonine kinase 1 (AKT1), vascular endothelial growth factor A (VEGFA), cyclin D1 (CCND1) and estrogen receptor 1 (ESR1), were screened out, which were closely related to adipogenesis, fatty acid metabolism and bile acid metabolism. The subsequent animal experiments showed that AM extract treatment improved the lipid profiles of the high-fat diet (HFD)-fed mice by reducing lipogenesis and increasing lipolysis and lipid β-oxidation, which were associated with the downregulating of AKT1 and CCND1, and the upregulating of VEGFA and ESR1 in liver and adipose tissue. Overall, AM alleviated acquired hyperlipidemia through regulating lipid metabolism, and AKT1, VEGFA, CCND1 and ESR1 might be the key targets.
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http://dx.doi.org/10.3390/nu14050954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912611PMC
February 2022

Molecular basis of receptor binding and antibody neutralization of Omicron.

Nature 2022 04 28;604(7906):546-552. Epub 2022 Feb 28.

State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.

The SARS-CoV-2 Omicron variant exhibits striking immune evasion and is spreading rapidly worldwide. Understanding the structural basis of the high transmissibility and enhanced immune evasion of Omicron is of high importance. Here, using cryo-electron microscopy, we present both the closed and the open states of the Omicron spike (S) protein, which appear more compact than the counterparts of the G614 strain, potentially related to enhanced inter-protomer and S1-S2 interactions induced by Omicron residue substitution. The closed state showing dominant population may indicate a conformational masking mechanism for the immune evasion of Omicron. Moreover, we captured three states for the Omicron S-ACE2 complex, revealing that the substitutions on the Omicron RBM result in new salt bridges and hydrogen bonds, more favourable electrostatic surface properties, and an overall strengthened S-ACE2 interaction, in line with the observed higher ACE2 affinity of Omicron S than of G614. Furthermore, we determined the structures of Omicron S in complex with the Fab of S3H3, an antibody that is able to cross-neutralize major variants of concern including Omicron, elucidating the structural basis for S3H3-mediated broad-spectrum neutralization. Our findings shed light on the receptor engagement and antibody neutralization or evasion of Omicron and may also inform the design of broadly effective vaccines against SARS-CoV-2.
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http://dx.doi.org/10.1038/s41586-022-04581-9DOI Listing
April 2022

Outflow reconstruction of left lateral graft with two widely spaced hepatic veins in pediatric living donor liver transplantation.

Surgery 2022 Jul 23;172(1):391-396. Epub 2022 Feb 23.

Pediatric Transplant Department, Tianjin First Central Hospital, China; The Key Subject of Tianjin First Central Hospital, China. Electronic address:

Background: Living donor liver transplantation using the left lateral segment of the liver is the most common type of pediatric liver transplantation. An appropriate surgical approach is crucial for decreasing the risk of vascular complications using these grafts with anatomical variations.

Methods: Between January 2017 and December 2020, 631 living donor liver transplantations using left lateral segment grafts were performed at Tianjin First Central Hospital. The grafts from 162 (25.7%) donors have 2 hepatic vein openings. A total number of 21 transplantations using left lateral segment grafts with 2 widely spaced hepatic vein openings were performed. In group 1, the unification venoplasty technique with interposition vein graft was used at the back table for the reconstruction of hepatic vein from grafts. In group 2, dual hepatic vein reconstructions were performed, in which venoplasty of recipients' left hepatic vein, middle hepatic vein, and inferior vena cava was performed to create a large orifice for anastomosis with segment Ⅱ hepatic vein from the graft. Segment III hepatic vein from the graft was anastomosed with the recipient's right hepatic vein. The incidence, treatment, and outcomes of hepatic venous outflow obstruction were compared between the 2 groups.

Results: The median follow-up time was 12.8 months. There was no significant difference in the incidence of hepatic venous outflow obstruction between the 2 groups.

Conclusion: Dual hepatic vein reconstruction is an alternate surgical option for grafts with 2 widely spaced hepatic veins, and it is associated with ideal graft recovery and vascular condition. However, long-term follow-up is still needed to verify the efficacy and safety of this approach.
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http://dx.doi.org/10.1016/j.surg.2022.01.026DOI Listing
July 2022

Prediction for Mitosis-Karyorrhexis Index Status of Pediatric Neuroblastoma via Machine Learning Based F-FDG PET/CT Radiomics.

Diagnostics (Basel) 2022 Jan 20;12(2). Epub 2022 Jan 20.

Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.

Accurate differentiation of intermediate/high mitosis-karyorrhexis index (MKI) from low MKI is vital for the further management of neuroblastoma. The purpose of this research was to investigate the efficacy of F-FDG PET/CT-based radiomics features for the prediction of MKI status of pediatric neuroblastoma via machine learning. A total of 102 pediatric neuroblastoma patients were retrospectively enrolled and divided into training (68 patients) and validation sets (34 patients) in a 2:1 ratio. Clinical characteristics and radiomics features were extracted by XGBoost algorithm and were used to establish radiomics and clinical models for MKI status prediction. A combined model was developed, encompassing clinical characteristics and radiomics features and presented as a radiomics nomogram. The predictive performance of the models was evaluated by AUC and decision curve analysis. The radiomics model yielded AUC of 0.982 (95% CI: 0.916, 0.999) and 0.955 (95% CI: 0.823, 0.997) in the training and validation sets, respectively. The clinical model yielded AUC of 0.746 and 0.670 in the training and validation sets, respectively. The combined model demonstrated AUC of 0.988 (95% CI: 0.924, 1.000) and 0.951 (95% CI: 0.818, 0.996) in the training and validation sets, respectively. The radiomics features could non-invasively predict MKI status of pediatric neuroblastoma with high accuracy.
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http://dx.doi.org/10.3390/diagnostics12020262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871335PMC
January 2022

Cardiac Rehabilitation Improves Long-Term Prognosis for People with Chronic Kidney Disease Undergoing Percutaneous Coronary Intervention: A Propensity Matching Analysis.

J Healthc Eng 2022 9;2022:1196682. Epub 2022 Feb 9.

Department of Emergency Department, First People's Hospital of Chongqing Liangjiang New Area, Chongqing, China.

Objectives: According to researches, many people with chronic kidney disease (CKD) had the higher incidence rate and mortality rate of coronary artery disease (CAD) after percutaneous coronary intervention than those who did not receive percutaneous coronary intervention, while coronary rehabilitation was beneficial for patients who received percutaneous coronary intervention I. This study aims to analyze whether coronary rehabilitation was beneficial to patients with CKD after percutaneous coronary intervention. . A retrospective survey was used to collect clinical data of patients undergoing percutaneous coronary intervention due to CAD, and CKD patients were screened for further analysis. According to whether patients had received coronary rehabilitation treatment, research subjects were divided into two groups, the coronary rehabilitation group and the noncoronary rehabilitation group. The baseline characteristics of the propensity score matching between the two groups were compared. Survival analysis used the Cox hazard ratio (HR) model as regression method to compare the relative risk of the endpoints in the coronary rehabilitation group and the noncoronary rehabilitation group.

Results: From January 2007 to January 2012, a total of 246 CKD patients treated with percutaneous coronary intervention were included in this study, and 106 of them obtained coronary rehabilitation after surgery. After propensity score matching, there were 89 pairs of patients in the two groups who had no significant difference in demographic and clinical characteristics (all > 0.05). CKD patients receiving coronary rehabilitation had a significant reduction in all-cause mortality (HR 0.465, 95% CI 0.233-0.926, =0.029) and cardiac complications (HR 0.532, 95% CI 0.287-0.984, =0.044). Survival analysis showed that the survival rate of the coronary rehabilitation group was significantly higher than that of the noncoronary rehabilitation group (=0.024).

Conclusions: For CKD patients undergoing percutaneous coronary intervention, receiving cardiac rehabilitation can significantly improve long-term survival and reduce cardiac events.
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http://dx.doi.org/10.1155/2022/1196682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8849798PMC
April 2022

Structural basis for SARS-CoV-2 Delta variant recognition of ACE2 receptor and broadly neutralizing antibodies.

Nat Commun 2022 02 15;13(1):871. Epub 2022 Feb 15.

State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.

The SARS-CoV-2 Delta variant is currently the dominant circulating strain in the world. Uncovering the structural basis of the enhanced transmission and altered immune sensitivity of Delta is particularly important. Here we present cryo-EM structures revealing two conformational states of Delta spike and S/ACE2 complex in four states. Our cryo-EM analysis suggests that RBD destabilizations lead to population shift towards the more RBD-up and S1 destabilized fusion-prone state, beneficial for engagement with ACE2 and shedding of S1. Noteworthy, we find the Delta T478K substitution plays a vital role in stabilizing and reshaping the RBM loop, enhancing interaction with ACE2. Collectively, increased propensity for more RBD-up states and the affinity-enhancing T478K substitution together contribute to increased ACE2 binding, providing structural basis of rapid spread of Delta. Moreover, we identify a previously generated MAb 8D3 as a cross-variant broadly neutralizing antibody and reveal that 8D3 binding induces a large K478 side-chain orientation change, suggesting 8D3 may use an "induced-fit" mechanism to tolerate Delta T478K mutation. We also find that all five RBD-targeting MAbs tested remain effective on Delta, suggesting that Delta well preserves the neutralizing antigenic landscape in RBD. Our findings shed new lights on the pathogenicity and antibody neutralization of Delta.
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http://dx.doi.org/10.1038/s41467-022-28528-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847413PMC
February 2022

Sesamol Reverses Myofiber-Type Conversion in Obese States via Activating the SIRT1/AMPK Signal Pathway.

J Agric Food Chem 2022 Feb 15;70(7):2253-2264. Epub 2022 Feb 15.

Department of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, Hunan Province China.

Obesity can evoke changes of skeletal muscle structure and function, which are characterized by the conversion of myofiber from type I to type II, leading to a vicious cycle of metabolic disorders. Reversing the muscle fiber-type conversion in obese states is a novel strategy for treating those with obesity. Sesamol, a food ingredient compound isolated from sesame seeds, exerted potential antiobesity effects. The present research aimed to explore the therapeutic effects of sesamol on obesity-related skeletal muscle-fiber-type conversion and elucidate the underlying molecular mechanisms through utilizing a high-fat-diet-induced obese C57BL/6J mice model and palmitic acid-exposed C2C12 myotubes. The results showed that sesamol attenuated obesity-related metabolic disturbances, elevated exercise endurance of obese mice, and decreased lipid accumulation and insulin resistance in skeletal muscle. After the treatment with sesamol, the muscular mitochondrial content and biogenesis were increased, accompanied by the enzyme activities and myosin heavy-chain isoform changed from type II fiber to type I fiber. Mechanistic studies revealed that the effects of sesamol on reversing skeletal muscle-fiber-type conversion in obese states were associated with the stimulation of the muscular sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) signal pathway, and these effects could be inhibited by a specific inhibitor of SIRT1, EX-527. In conclusion, our research provided novel evidence that sesamol could regulate myofiber-type conversion to treat obesity and obesity-related metabolic disorders by stimulating the muscular SIRT1/AMPK signal pathway.
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http://dx.doi.org/10.1021/acs.jafc.1c08036DOI Listing
February 2022
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