Publications by authors named "Hong Liu"

4,815 Publications

  • Page 1 of 1

Nearly-Unity Quantum Yield and 12-Hour Afterglow from a Transparent Perovskite of Cs2NaScCl6:Tb.

Angew Chem Int Ed Engl 2022 Aug 11. Epub 2022 Aug 11.

University of Jinan, Institute for Advanced Interdisciplinary Research, Tejiao Building, 336# Nanxinzhuang Xilu, 250022, Jinan, CHINA.

Unlike afterglow phosphors, transparent crystal free of scattering issue held great potential in specific applications such as volumetric display and three-dimensional encryption. Here, a double-perovskite host of ~ 85% transparency, namely Cs2NaScCl6 single crystal, was grown in an aqueous solution. Through heavy doping of Tb ions, the crystal exhibited a high photoluminescence quantum yield (PL QY) up to 98.2%. Both steady-state and transient PL spectroscopic investigation revealed a cross-relaxation efficiency (ηCR ~ 99%) between Tb ions, which greatly mitigated the electron saturation on the 5D3 level and encouraged the radiative route against quenching. Importantly, the doped crystal displayed an intense afterglow up to 12 h after ceasing the excitation of X-ray, which inspired the design of a radiation-storage battery of linear scaling capacity with loading weight. Our work opened many possibilities in photo-stimulated volumetric display and three-dimensional information storage.
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http://dx.doi.org/10.1002/anie.202210853DOI Listing
August 2022

Multi-omics characterization and therapeutic liability of ferroptosis in melanoma.

Signal Transduct Target Ther 2022 Aug 10;7(1):268. Epub 2022 Aug 10.

Department of Dermatology, Hunan Key Laboratory of Skin Cancer and Psoriasis, Hunan Engineering Research Center of Skin Health and Disease, Xiangya Clinical Research Center for Cancer Immunotherapy, Xiangya Hospital, Central South University, Changsha, China.

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http://dx.doi.org/10.1038/s41392-022-01067-yDOI Listing
August 2022

Identification of linear epitopes and their major role in the immunoglobulin E-binding capacity of tropomyosin from .

Food Funct 2022 Aug 9. Epub 2022 Aug 9.

College of Ocean Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, Fujian 361021, China.

Tropomyosin (TM) is an important allergen in molluscans. However, there was a lack of information about TM as an allergen in oysters. TM was purified and identified from (ATM), and its primary sequence was cloned and encoded with 284 amino acids (AAs). Chemical denaturants were used to destroy the structure to confirm that linear epitopes played a major role in the immunoglobulin E-binding capacity of ATM. Subsequently, nine linear epitopes were identified using a serological test. The peptide with AA was regarded as the key epitope because it could be recognized strongly by most sera of oyster-sensitive individuals in comparison to other epitope peptides. Finally, the epitopes and the primary sequence of TM among shellfish were aligned to find the two conserved epitopes (AA and AA) in oyster, octopus, abalone, scallop, clam, shrimp, and crab. Overall, these data provide a foundation for the allergenicity and cross-reactivity of TM.
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http://dx.doi.org/10.1039/d2fo01713jDOI Listing
August 2022

Development and validation of a multiplex typing system with 32 Y-STRs for forensic application.

Forensic Sci Int 2022 Jul 22;339:111409. Epub 2022 Jul 22.

Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangdong, China; Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, China; Guangzhou Forensic Science Institute, Guangzhou, China. Electronic address:

Y-chromosomal short tandem repeats (Y-STRs) with high mutation rates will increase the potential to distinguish related males and improve discrimination capacity. The newly developed Y32 kit is a six-dye multiplex amplification kit that contains 21 rapidly mutating Y-STR loci (DYF387S1a/b, DYF399S1a/b/c, DYF403S1a1/a2/a3/b, DYF404S1a/b, DYS449, DYS518, DYS526a/b, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627), 8 fast mutating Y-STR loci (DYS458, DYS464a/b/c/d, DYS516, DYS534, and DYS713), and 3 moderately mutating Y-STR loci (Y-GATA-A10, DYS630, and DYS446). To verify the efficiency and accuracy of the Y32 kit, PCR reaction conditions, sensitivity, mixture, concordance, inhibition, species specificity, case samples, reproducibility, sizing precision, stutter, and population study were studied according to the SWGDAM developmental validation guidelines. The results showed that the Y32 kit is efficient, accurate, reliable, and highly informative for forensic applications.
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http://dx.doi.org/10.1016/j.forsciint.2022.111409DOI Listing
July 2022

Role of diffusion-weighted imaging in differentiating angiomatous meningioma from atypical meningioma.

Clin Neurol Neurosurg 2022 Aug 2;221:107406. Epub 2022 Aug 2.

Radiology of Department, Lanzhou University Second Hospital, Cuiyingmen No.82, Chengguan District, Lanzhou 730030, People's Republic of China; Second Clinical School, Lanzhou University, Lanzhou, People's Republic of China; Key Laboratory of Medical Imaging of Gansu Province, Lanzhou, People's Republic of China; Gansu International Scientific and Technological Cooperation Base of Medical Imaging Artificial Intelligence, Lanzhou, People's Republic of China. Electronic address:

Background: Conventional magnetic resonance imaging (MRI) characteristics of angiomatous meningioma (AM) and atypical meningioma (ATM) sometimes overlap. However, there are significant differences in the treatment and prognosis of the two tumors. The aim of the study was to assess the role of diffusion-weighted imaging (DWI) in differentiating AM from ATM.

Methods: Clinical, MRI, and pathological data of 25 patients with AM and 30 patients with ATM were retrospectively analyzed. Main clinical indexes, conventional MRI characteristics, and apparent diffusion coefficient (ADC) values were compared between the two groups, and receiver operating characteristic (ROC) curves were drawn to determine the diagnostic performance of ADC values in distinguishing AM from ATM.

Results: The minimum ADC value (ADCmin), average ADC value (ADCmean), and relative ADC value (rADC) for AM (908.00 ± 117.00 × 10 mm/s, 921.04 ± 67.09 × 10 mm/s, and 1.15 ± 0.09, respectively) were significantly higher than those for ATM (710.50 ± 79.80 × 10 mm/s, 748.50 ± 67.27 × 10 mm/s, and 0.96 ± 0.09, respectively; all P < 0.05). ROC analysis showed that ADCmin had the best diagnostic performance in distinguishing AM from ATM, with an area under the curve value of 0.977. When using 759.00 × 10 mm/s as the optimal threshold, the sensitivity, specificity, accuracy, positive predictive value, and negative predictive value were 90.00 %, 96.00 %, 92.72 %, 96.40 %, and 88.90 %, respectively.

Conclusions: DWI plays an important role in differentiating AM from ATM, and ADCmin is the most promising potential parameter that can improve the preoperative diagnostic accuracy of both tumors.
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http://dx.doi.org/10.1016/j.clineuro.2022.107406DOI Listing
August 2022

Characterization of immune-related alternative polyadenylation events in cancer immunotherapy.

Cancer Res 2022 Aug 5. Epub 2022 Aug 5.

Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Alternative polyadenylation (APA) is an important post-transcriptional modification commonly involved in tumor development. However, the functional roles of APA in tumor immunity remain largely unknown. Here, we performed an in-depth analysis of the 3'UTR usage of protein-coding genes and tumor immune response in 10,303 tumor samples across 31 cancer types to develop the immune-related APA event (ImmAPA) score pipeline, an integrated algorithm to characterize the regulatory landscape of APA events in cancer immunity-related pathways. Tumor-specific ImmAPAs that strongly correlate with immune cell infiltration and immune checkpoint blockade (ICB) treatment-related biomarkers were identified. Among these ImmAPAs, the top-ranking COL1A1 3'UTR usage was strongly associated with worse prognosis and tumor immune evasion. Furthermore, a machine learning approach to construct an ICB-related ImmAPA score model predicted immunotherapy efficacy. Overall, the characterization of immune-related APA that correspond to tumor progression and tumor immunity highlights the clinical utility of APA events as potential biomarkers in cancer immunotherapy.
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http://dx.doi.org/10.1158/0008-5472.CAN-22-1417DOI Listing
August 2022

Effects of length and type of the alkyl chain on the micellization behavior of mixed systems of HS15 with fatty acids.

Food Chem 2022 Jul 30;397:133830. Epub 2022 Jul 30.

College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Pharmaceutical Process and Quality Control, Chongqing 400715, China. Electronic address:

Due to volatility, low solubility and instability, the application of SCFAs and MCFAs is limited, which is expected to be solved by micelles. Taking SCFAs and MCFAs as models, this paper aims to research the influences of alkyl chain length and type on HS15 micelles. The critical micelle concentration (CMC) of various acid-HS15 systems was determined firstly. Then some air-water interface parameters and thermodynamic parameters were analyzed. Subsequently, particle size, cloud points (CP) and fluorophore release curves were measured. With the increase of CMC and G, the decrease of size and CP, and the rapid quenching of fluorophores, it is more difficult for acids with longer-chain to form stable micelles with HS15 because of the strength of iceberg structure. Showing smaller CMC and G, smaller size, higher CP and slower release of fluorescers, branched molecules can bind more closely to the hydrophobic part of HS15 due to their spatial flexibility.
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http://dx.doi.org/10.1016/j.foodchem.2022.133830DOI Listing
July 2022

Rh(III)-Catalyzed C-H Activation and [4+1+1] Sequential Cyclization Cascade to Give Highly Fused Indano[1,2-]azirines.

Org Lett 2022 Aug 3;24(31):5688-5692. Epub 2022 Aug 3.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China.

A Rh(III)-catalyzed C-H activation of α-keto oximes and a cyclization cascade with diazo compounds were developed to construct the highly fused indano[1,2-]azirine frameworks in good yields with a broad range of substrates under mild reaction conditions. More intriguingly, a [4+1+1] sequential annulation cascade is demonstrated for the first time in this reaction and opened a new reaction mode for α-keto oximes. These fused indano[1,2-]azirine derivatives could also be further transformed into intriguing privileged drug scaffolds.
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http://dx.doi.org/10.1021/acs.orglett.2c02070DOI Listing
August 2022

Ultrahigh Performance Triboelectric Nanogenerator Enabled by Charge Transmission in Interfacial Lubrication and Potential Decentralization Design.

Research (Wash D C) 2022 5;2022:9812865. Epub 2022 Jul 5.

School of Physics, State Key Laboratory of Power Transmission Equipment and System Security and New Technology, Chongqing University, Chongqing 400044, China.

Triboelectric nanogenerator (TENG) is a promising strategy for harvesting low frequency mechanical energy. However, the bottlenecks of limited electric output by air/dielectric breakdown and poor durability by material abrasion seriously restrict its further improvement. Herein, we propose a liquid lubrication promoted sliding mode TENG to address both issues. Liquid lubrication greatly reduces interface material abrasion, and its high breakdown strength and charge transmission effect further enhance device charge density. Besides, the potential decentralization design by the voltage balance bar effectively suppresses the dielectric breakdown. In this way, the average power density up to 87.26 W·m·Hz, energy conversion efficiency of 48%, and retention output of 90% after 500,000 operation cycles are achieved, which is the highest average power density and durability currently. Finally, a cell phone is charged to turn on by a palm-sized TENG device at 2 Hz within 25 s. This work has a significance for the commercialization of TENG-based self-powered systems.
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http://dx.doi.org/10.34133/2022/9812865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285635PMC
July 2022

Identification of Critical miRNAs as Novel Diagnostic Markers for Laryngeal Squamous Cell Carcinoma.

Dis Markers 2022 20;2022:6858411. Epub 2022 Jul 20.

Department of Head and Neck Cancer Center, Chongqing University Cancer Hospital, Chongqing 400030, China.

The prognosis of laryngeal squamous cell carcinoma (LSCC) patients remains poor, and early diagnosis can distinctly improve the long-term survival of LSCC patients. MicroRNAs (miRs) are a group of endogenous, noncoding, 18-24 nucleotide length single-strand RNAs and have been demonstrated to regulate the expression of many genes, thus modulating various cellular biological processes. In this study, we aimed to identify critical diagnostic miRNAs based on two machine learning algorithms. The GSE133632 dataset was acquired from the Gene Expression Omnibus (GEO) datasets, comprising LSCC tissular samples (57 specimens) and matched neighboring healthy mucosa tissular samples (57 specimens). Differentially expressed miRNAs (DEMs) were screened between 57 LSCC specimens and 57 normal specimens. The LASSO regression model and SVM-RFE analysis were carried out for the identification of critical miRNAs. ROC assays were applied to evaluate discriminatory ability. We identified 32 DEMs between LSCC specimens and normal specimens. Two machine learning algorithms confirmed that hsa-miR-615-3p, hsa-miR-4652-5p, hsa-miR-450a-5p, hsa-miR-196a-5p, hsa-miR-21-3p, hsa-miR-139-5p, and hsa-miR-424-5p were critical diagnostic factors. According to the ROC assays, seven miRNAs had an AUC value of >0.85 for LSCC. Taken together, our findings identified seven critical miRNAs in LSCC patients which can be used to diagnose LSCC patients with high sensitivity and specificity. These results must be verified by large-scale prospective studies.
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http://dx.doi.org/10.1155/2022/6858411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329032PMC
August 2022

Metabolomics of clinical samples reveal the treatment mechanism of lanthanum hydroxide on vascular calcification in chronic kidney disease.

Proc Jpn Acad Ser B Phys Biol Sci 2022 ;98(7):361-377

Department of Pharmacology, College of Pharmacy, Inner Mongolian Medical University.

Previous studies showed that lanthanum hydroxide (LH) has a therapeutic effect on chronic kidney disease (CKD) and vascular calcification, which suggests that it might have clinical value. However, the target and mechanism of action of LH are unclear. Metabolomics of clinical samples can be used to predict the mechanism of drug action. In this study, metabolomic profiles in patients with end-stage renal disease (ESRD) were used to screen related signaling pathways, and we verified the influence of LH on the ROS-PI3K-AKT-mTOR-HIF-1α signaling pathway by western blotting and quantitative real-time RT-qPCR in vivo and in vitro. We found that ROS and SLC16A10 genes were activated in patients with ESRD. The SLC16A10 gene is associated with six significant metabolites (L-cysteine, L-cystine, L-isoleucine, L-arginine, L-aspartic acid, and L-phenylalanine) and the PI3K-AKT signaling pathway. The results showed that LH inhibits the ESRD process and its cardiovascular complications by inhibiting the ROS-PI3K-AKT-mTOR-HIF-1α signaling pathway. Collectively, LH may be a candidate phosphorus binder for the treatment of vascular calcification in ESRD.
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http://dx.doi.org/10.2183/pjab.98.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9363596PMC
August 2022

Research note: genetic diversity of duck circoviruses circulating in partial areas of Guangdong province, southern China.

Poult Sci 2022 Jun 26;101(9):102032. Epub 2022 Jun 26.

School of Life Science and Engineering, Foshan University, Foshan 528225, Guangdong province, China; Foshan University Veterinary Teaching Hospital, Foshan 528225, Guangdong province, China. Electronic address:

Duck circovirus (DuCV) is the smallest known virus in waterfowl that infects both domestic and wild duck. Infected ducks often show stunted growth and immunosuppression, which increases the rate of secondary infection with other pathogens. In this study, 270 liver tissue samples were collected to screen the presence of DuCV in Guangdong province, China, and the complete genome sequences were recovered and systematically analyzed. Genetic analyses revealed that sequences determined in this study shared 81.6% to 100.0% genome-wide pairwise identity with previously identified DuCV genomes. Phylogenetic analyses showed that 2 DuCV genotypes with a high infection rate were co-circulating in duck population in Guangdong province, and extensive recombination events have occurred during the evolution of DuCV. Our results expand upon the knowledge regarding the genetic diversity and evolution of DuCV, and also indicate that extensive genetically divergent DuCV are co-circulating in the duck populations in Guangdong, southern China.
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http://dx.doi.org/10.1016/j.psj.2022.102032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334309PMC
June 2022

Multi-Phenotypic Exosome Secretion Profiling Microfluidic Platform for Exploring Single-Cell Heterogeneity.

Small Methods 2022 Jul 28:e2200717. Epub 2022 Jul 28.

Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong, 266000, China.

Cellular phenotypic and functional heterogeneities have advanced cancer evolution and treatment resistance. Although exosome-bound proteins reflect cellular functions, single-cell exosomes are rarely profiled owing to the lack of effective platforms. Herein, the authors developed an integrated microfluidic platform consisting of a single-cell trapping chip and a spatially coded antibody barcode chip for the multiplexed outline of exosome secretion by single cells. Using this platform, five phenotypic exosomes of over 1 000 single cells are simultaneously profiled, in addition to inflammatory factor secretion from the same single cell. Also, a robust analysis workflow for single-cell secretion profiling is proposed to explore the intercellular heterogeneity, which integrated unsupervised clustering and linear clustering. When applied to the tumor cell lines of epithelial-origin and normal epithelial cell lines, the strategy identifies functionally heterogeneous subpopulations with unique secretion patterns. Notably, special functional cell subsets for unique phenotypic exosomes (HSP70 , EPCAM ) are found within ovarian tumor cells. The strategy proposed offers a new analysis approach for cellular differential exosome secretion at single-cell resolution using inflammatory factors, ultimately reinforcing the understanding of cell-to-cell heterogeneity and tumor landscape, and providing a valuable universal platform for single-cell biomarker exploration in biological and clinical research.
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http://dx.doi.org/10.1002/smtd.202200717DOI Listing
July 2022

Locoregional Therapy in De novo Metastatic Breast Cancer: A Retrospective Cohort Study.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338221115356

Cangzhou Clinical College of Integrated Traditional Chinese and Western Medicine of Hebei Medical University, Cang Zhou, China.

Whether locoregional therapy (LRT) should be performed in patients with de novo metastatic breast cancer (dnMBC) has been debated. Here we report the survival outcomes of LRT in patients with dnMBC, focusing on the association of surgical timings and surgical margins with survival in this patient population. The retrospective study included patients with dnMBC in our hospital, between March 1, 2007 and December 31, 2017. Overall survival (OS) was evaluated by means of a stratified log-rank test and summarized with the use of Kaplan-Meier methods. A total of 153 patients were included, of whom 87 underwent LRT and 66 systemic therapy alone (STA). LRT showed a significant OS benefit over STA (HR, 0.62; 95% CI, 0.39 to 0.99;   =  .043). The OS of LRT group and STA group were 39 months (95% CI, 33.6 to 44.4 months) and 24.6 months (95% CI, 20.6 to 28.6 months), respectively. The benefit was consistent across most subgroups. The OS of patients undergoing surgery was better than that of patients without surgery (HR, 0.58; 95% CI, 0.35 to 0.94;   =  .0001), and there was difference in survival improvement at different surgical timings (surgery before chemotherapy, during chemotherapy, and after chemotherapy) (  =  .0001). The survival benefit of surgery after chemotherapy was significantly greater than that of the other 2 options (HR, 0.77; 95% CI, 0.63 to 0.94). Moreover, compared with patients with positive margins, the OS of patients with negative margins was significantly improved (HR, 0.22; 95% CI, 0.13 to 0.37;  < .001). Our results suggest that LRT is associated with improved OS in women with dnMBC, and patients who had surgery after systemic chemotherapy with negative surgical margins, are expected to benefit more.
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http://dx.doi.org/10.1177/15330338221115356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340403PMC
July 2022

Toxicity of different forms of antimony to rice plants: Photosynthetic electron transfer, gas exchange, photosynthetic efficiency, and carbon assimilation combined with metabolome analysis.

J Hazard Mater 2022 09 21;437:129433. Epub 2022 Jun 21.

Institute of Environmental Microbiology, College of Resources and Environment, Fujian Agriculture & Forestry University, Fuzhou 350002, China. Electronic address:

Antimony (Sb) is a toxic metalloid, and excess Sb causes damage to the plant photosynthetic system. However, the underlying mechanisms of Sb toxicity in the plant photosynthetic system are not clear. Hydroponic culture experiments were conducted to illustrate the toxicity differences of antimonite [Sb(III)] and antimonate [Sb(V)] to the photosynthetic system in a rice plant (Yangdao No. 6). The results showed that Sb(III) showed a higher toxicity than Sb(V), judging from (1) lower shoot and root biomass, leaf water moisture content, water use efficiency, stomatal conductance, net photosynthetic rate, and transpiration rate; (2) higher water vapor deficit, soluble sugar content, starch content, and oligosaccharide content (sucrose, stachyose, and 1-kestose). To further analyze the direction of the photosynthetic products, we conducted a metabonomic analysis. More glycosyls were allocated to the synthesis pathways of oligosaccharides (sucrose, stachyose, and 1-kestose), anthocyanins, salicylic acid, flavones, flavonols, and lignin under Sb stress to quench excess oxygen free radicals (ROS), strengthen the cell wall structure, rebalance the cell membrane, and/or regulate cell permeability. This study provides a complete mechanism to elucidate the toxicity differences of Sb(III) and Sb(V) by exploring their effects on photosynthesis, saccharide synthesis, and the subsequent flow directions of glycosyls.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129433DOI Listing
September 2022

Codelivery of HBx-siRNA and Plasmid Encoding IL-12 for Inhibition of Hepatitis B Virus and Reactivation of Antiviral Immunity.

Pharmaceutics 2022 Jul 9;14(7). Epub 2022 Jul 9.

National & Local Joint Engineering Research Center of High-Throughput Drug Screening Technology, College of Life Sciences, Hubei University, Wuhan 430062, China.

Chronic hepatitis B is a critical cause of many serious liver diseases such as hepatocellular carcinoma (HCC). The main challenges in hepatitis B treatment include the rebound of hepatitis B virus (HBV)-related antigen levels after drug withdrawal and the immunosuppression caused by the virus. Herein, we demonstrate that the HBV-related antigen can be effectively inhibited and antiviral immunity can be successfully reactivated through codelivery of the small interfering RNA (siRNA) targeting HBV X protein (HBx) and the plasmid encoding interleukin 12 (pIL-12) to hepatocytes and immune cells. After being treated by the siRNA/pIL-12 codelivery system, HBx mRNA and hepatitis B surface antigen (HBsAg) are dramatically reduced in HepG2.215 cells. More importantly, the downregulated CD47 and programmed death ligand 1 (PD-L1) and the upregulated interferon-β promoter stimulator-1 (IPS-1), retinoic acid-inducible gene-1 (RIG-1), CD80, and human leukocyte antigen-1 (HLA-1) in treated HepG2.215 cells indicate that the immunosuppression is reversed by the codelivery system. Furthermore, the codelivery system results in inhibition of extracellular regulated protein kinases (ERK) and phosphoinositide-3-kinase (PI3K)/protein kinase B (Akt) pathways, as well as downregulation of B-cell lymphoma-2 (Bcl-2) and upregulation of p53, implying its potential in preventing the progression of HBV-induced HCC. In addition, J774A.1 macrophages treated by the codelivery system were successfully differentiated into the M1 phenotype and expressed enhanced cytokines with anti-hepatitis B effects such as interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α). Therefore, we believe that codelivery of siRNA and pIL-12 can effectively inhibit hepatitis B virus, reverse virus-induced immunosuppression, reactivate antiviral immunity, and hinder the progression of HBV-induced hepatocellular carcinoma. This investigation provides a promising approach for the synergistic treatment of HBV infection.
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http://dx.doi.org/10.3390/pharmaceutics14071439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318813PMC
July 2022

Tumor-Derived Membrane Vesicles: A Promising Tool for Personalized Immunotherapy.

Pharmaceuticals (Basel) 2022 Jul 16;15(7). Epub 2022 Jul 16.

Zhuhai Jinan Selenium Source Nanotechnology Co., Ltd., Jinan University, Zhuhai 519000, China.

Tumor-derived membrane vesicles (TDMVs) are non-invasive, chemotactic, easily obtained characteristics and contain various tumor-borne substances, such as nucleic acid and proteins. The unique properties of tumor cells and membranes make them widely used in drug loading, membrane fusion and vaccines. In particular, personalized vectors prepared using the editable properties of cells can help in the design of personalized vaccines. This review focuses on recent research on TDMV technology and its application in personalized immunotherapy. We elucidate the strengths and challenges of TDMVs to promote their application from theory to clinical practice.
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http://dx.doi.org/10.3390/ph15070876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318328PMC
July 2022

Improved Electron Efficiency of Zero-Valent Iron towards Cr(VI) Reduction after Sequestering in AlO Microspheres.

Int J Environ Res Public Health 2022 Jul 8;19(14). Epub 2022 Jul 8.

Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Institute of Environmental Research at Greater Bay, Guangzhou University, Guangzhou 510006, China.

Zero-valent iron (ZVI) is widely used for groundwater remediation, but suffers from high electron consumption because of its free contact with non-target substrates such as O. Here, ZVI-ALOX particles were prepared via in situ NaBH aqueous-phase reduction of ferrous ions (Fe) preabsorbed into AlO microspheres. The electron efficiency (EE) and long-term performance of the material were improved by sequestering ZVI in the interspace of the AlO microspheres (ZVI-ALOX). During long-term (350 days) continuous flow, Cr(VI) was removed to below the detection limit for over 23 days. Based on the high reactivity of ZVI towards Cr(VI), the EE of ZVI-ALOX was evaluated by measuring its Cr(VI) removal efficiency at neutral pH and comparing it with that of ZVI. The results showed that the EE of ZVI-ALOX during long-term continuous flow could reach 39.1%, which was much higher than that of ZVI (8.68%). The long-term continuous flow results also demonstrated that treatment of the influent solution achieved higher EE values than in the batch mode, where the presence of dissolved oxygen reduced EE values. At lower pollutant concentrations, the sequestering of ZVI was beneficial to its performance and long-term utility. In addition, measurement of the acute toxicity of treated column effluent using the indicator organism T showed that ZVI-ALOX could reduce the toxicity of 5 mg/L Cr(VI) solution by ~70% in 350 d. The results from this study provide a basis for the development of permeable reactive barriers for groundwater remediation based on sequestered ZVI.
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http://dx.doi.org/10.3390/ijerph19148367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316081PMC
July 2022

Transformers Improve Breast Cancer Diagnosis from Unregistered Multi-View Mammograms.

Diagnostics (Basel) 2022 Jun 25;12(7). Epub 2022 Jun 25.

School of Electrical and Computer Engineering, University of Oklahoma, Norman, OK 73019, USA.

Deep convolutional neural networks (CNNs) have been widely used in various medical imaging tasks. However, due to the intrinsic locality of convolution operations, CNNs generally cannot model long-range dependencies well, which are important for accurately identifying or mapping corresponding breast lesion features computed from unregistered multiple mammograms. This motivated us to leverage the architecture of Multi-view Vision Transformers to capture long-range relationships of multiple mammograms from the same patient in one examination. For this purpose, we employed local transformer blocks to separately learn patch relationships within four mammograms acquired from two-view (CC/MLO) of two-side (right/left) breasts. The outputs from different views and sides were concatenated and fed into global transformer blocks, to jointly learn patch relationships between four images representing two different views of the left and right breasts. To evaluate the proposed model, we retrospectively assembled a dataset involving 949 sets of mammograms, which included 470 malignant cases and 479 normal or benign cases. We trained and evaluated the model using a five-fold cross-validation method. Without any arduous preprocessing steps (e.g., optimal window cropping, chest wall or pectoral muscle removal, two-view image registration, etc.), our four-image (two-view-two-side) transformer-based model achieves case classification performance with an area under ROC curve (AUC = 0.818 ± 0.039), which significantly outperforms AUC = 0.784 ± 0.016 achieved by the state-of-the-art multi-view CNNs ( = 0.009). It also outperforms two one-view-two-side models that achieve AUC of 0.724 ± 0.013 (CC view) and 0.769 ± 0.036 (MLO view), respectively. The study demonstrates the potential of using transformers to develop high-performing computer-aided diagnosis schemes that combine four mammograms.
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http://dx.doi.org/10.3390/diagnostics12071549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320758PMC
June 2022

A Novel Early Warning Model for Hand, Foot and Mouth Disease Prediction Based on a Graph Convolutional Network.

Biomed Environ Sci 2022 Jun;35(6):494-503

NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100026, China;Center for Biosafety Mega Science, Chinese Academy of Sciences, Wuhan 430071, Hubei, China.

Objectives: Hand, foot and mouth disease (HFMD) is a widespread infectious disease that causes a significant disease burden on society. To achieve early intervention and to prevent outbreaks of disease, we propose a novel warning model that can accurately predict the incidence of HFMD.

Methods: We propose a spatial-temporal graph convolutional network (STGCN) that combines spatial factors for surrounding cities with historical incidence over a certain time period to predict the future occurrence of HFMD in Guangdong and Shandong between 2011 and 2019. The 2011-2018 data served as the training and verification set, while data from 2019 served as the prediction set. Six important parameters were selected and verified in this model and the deviation was displayed by the root mean square error and the mean absolute error.

Results: As the first application using a STGCN for disease forecasting, we succeeded in accurately predicting the incidence of HFMD over a 12-week period at the prefecture level, especially for cities of significant concern.

Conclusions: This model provides a novel approach for infectious disease prediction and may help health administrative departments implement effective control measures up to 3 months in advance, which may significantly reduce the morbidity associated with HFMD in the future.
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http://dx.doi.org/10.3967/bes2022.065DOI Listing
June 2022

Engineering the Electronic Structure of Ni3FeS with Polyaniline for Enhanced Electrocatalytic Performance of Overall Water Splitting.

Nanotechnology 2022 Jul 25. Epub 2022 Jul 25.

Shandong University, State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, Shandong, China, Jinan, Shandong, 250100, CHINA.

Developing highly efficient and stable electrocatalysts for oxygen evolution reaction is of significant importance for applications in energy conversion and storage. Modulation of electronic structure of catalysts is critical for improving the performance of the resulting electrodes. Here, we report a facile way to engineer the electronic structure of Ni3FeS by coating a thin polyaniline (PANI) layer for improving electrocatalytic activity for overall water splitting. Experimental investigations unveil that the strong electronic interactions between the lone electron pairs of nitrogen in PANI and d orbitals of iron, nickel in Ni3FeS result in an electron-rich structure of Ni and Fe, and consequently optimize the adsorption and desorption processes to promote the OER activity. Remarkably, the resulting PANI/Ni3FeS electrode exhibited much enhanced OER performance with a low overpotential of 143 mV at a current density of 10 mA·cm-2 and good stability. Promisingly, coupled with the reported MoNi4/MoO2 electrode, the two-electrode electrolyzer achieved a current density of 10 mA·cm-2 with a relatively low potential of 1.55V, and can generate oxygen and hydrogen bubbles steadily driven by a commercial dry battery, endowed the composite electrocatalyst with high potential for practical applications.
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http://dx.doi.org/10.1088/1361-6528/ac83cbDOI Listing
July 2022

Three-Dimensional-Cultured MSC-Derived Exosome-Hydrogel Hybrid Microneedle Array Patch for Spinal Cord Repair.

Nano Lett 2022 Aug 25;22(15):6391-6401. Epub 2022 Jul 25.

Department of Neurosurgery, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, P.R. China.

Exosomes derived from mesenchymal stem cells (MSCs) have been proven to exhibit great potentials in spinal cord injury (SCI) therapy. However, conventional two-dimensional (2D) culture will inevitably lead to the loss of stemness of MSCs, which substantially limits the therapeutic potency of MSCs exosomes (2D-Exo). Exosomes derived from three-dimensional culture (3D-Exo) possess higher therapeutic efficiency which have wide applications in spinal cord therapy. Typically, conventional exosome therapy that relies on local repeated injection results in secondary injury and low efficiency. It is urgent to develop a more reliable, convenient, and effective exosome delivery method to achieve constant exosomes release. Herein, we proposed a controlled 3D-exohydrogel hybrid microneedle array patch to achieve SCI repair . Our studies suggested that MSCs with 3D-culturing could maintain their stemness, and consequently, 3D-Exo effectively reduced SCI-induced inflammation and glial scarring. Thus, it is a promising therapeutic strategy for the treatment of SCI.
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http://dx.doi.org/10.1021/acs.nanolett.2c02259DOI Listing
August 2022

The Clinical Noteworthiness of Plasma NT-ProBNP Standard in Sufferers with Cardiogenic Cerebral Embolism and Its Diagnostic Value for Such Sufferers.

Contrast Media Mol Imaging 2022 1;2022:2536415. Epub 2022 Jul 1.

Neurology Department, Huaian Rehabilitation Hospital, Jinhu County People's Hospital, Jinhu 211600, China.

In order to explore the clinical noteworthiness of plasma NT-proBNP standards in sufferers with cardiogenic cerebral embolism and its diagnostic value for such sufferers, a retrospective study is conducted by the clinical data of sufferers with cerebral embolism. 100 sufferers with cerebral embolism admitted to our hospital from January 2018 to December 2020 are selected. According to the heparin-like drug therapy of acute ischemic stroke test (TOAST) classification criteria, they are divided into cardiac sufferers with cerebral embolism set (43 cases) and noncardiac cerebral embolism set (57 cases). The analysis results show the correlation between serum NT-proBNP standard and neurological impairment score. The detection of-proBNP standard can be used as a diagnostic indicator of disease severity and prognosis for sufferers with cardiogenic cerebral embolism.
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http://dx.doi.org/10.1155/2022/2536415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270157PMC
July 2022

Incremental value of myocardial deformation in predicting postnatal coarctation of the aorta: establishment of a novel diagnostic model.

J Am Soc Echocardiogr 2022 Jul 18. Epub 2022 Jul 18.

Department of Ultrasound Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Clinical Research Center for Medical Imaging in Hubei Province, Wuhan 430022, China; Hubei Province Key Laboratory of Molecular Imaging, Wuhan 430022, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518057, China; Tongji Medical College and Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

Background: Prenatal detection of coarctation of the aorta (CoA) still suffers from high false-positive and false-negative rates. The objective of this study was to develop a novel model to improve the diagnostic accuracy of fetal CoA.

Methods: A retrospective study was conducted in 122 fetuses with suspected CoA who also had postnatal follow-ups. Fetuses with confirmed diagnosis of CoA after birth were defined as CoA group, and Non-CoA group were those false-positives. Conventional fetal echocardiographic measurements, including great arterial dimensions and flow characteristics were obtained. Left ventricular (LV) functional parameters were determined using two-dimensional speckle tracking echocardiography. A novel multi-parametric diagnostic model, including gestational age (GA) at diagnosis, aortic isthmus (AOI) Z-score and LV longitudinal strain (LVLS), was developed by univariate and multivariate logistic regression analyses. The model was validated prospectively by a validation cohort of 48 fetuses.

Results: CoA was confirmed in 62/122 (50.8%) cases after birth. Fetuses with postnatal CoA were diagnosed significantly earlier than false-positives (median (interquartile range), 24.5 (23.3-26.4) vs 27.8 (24.5-30.4) weeks; P < .001). The Z-scores of aortic dimensions (aortic valve annulus, ascending aorta, transverse aortic arch and AOI) were significantly smaller (all P < .001), while the Z-scores of pulmonary dimensions (pulmonary valve annulus and main pulmonary artery) were significantly greater (all P < .05), in cases of confirmed CoA than false-positives. Compared with Non-CoA group, CoA group displayed lower LV ejection fraction (P = .005), LV fractional area change (P < .001) and LVLS (P < .001). A multivariate logistic regression model incorporating GA (odds ratio (OR): 0.74, 95% confidence interval (CI): 0.60-0.88; P = .001), AOI Z-score (OR: 0.20, 95% CI: 0.08-0.41; P < .001) and LVLS (OR: 1.79, 95% CI: 1.41-2.42; P < .001) was established to diagnose CoA more accurately (Akaike information criterion: 81.77, C-statistics: 0.945). The performance of this model was confirmed prospectively in the validation cohort.

Conclusions: In fetuses with suspected CoA, speckle tracking analysis of LVLS may have an incremental value in predicting postnatal CoA. Our diagnostic model, including GA, AOI Z-score and LVLS, may provide a good tool for the stratification of the risk in fetal CoA and contribute to patient-specific perinatal management.
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http://dx.doi.org/10.1016/j.echo.2022.07.010DOI Listing
July 2022

Epidemiology and Genetic Diversity of in Rodents in Urban Areas of Guangzhou, Southern China.

Front Microbiol 2022 4;13:942587. Epub 2022 Jul 4.

School of Life Science and Engineering, Foshan University, Foshan, China.

spp. are gram-negative bacteria that can infect a wide spectrum of mammals. Rodents are considered to be the natural reservoir of many species that are transmitted by various blood-sucking arthropods. The close contact between rodents and humans in urban areas increased the chance of transmitting rodent-borne to humans. Investigation of the epidemiological characteristics of infection in rodents is of great significance for the prevention and control of human Bartonellosis. In this study, rodents were captured to monitor the prevalence of in urban areas of Guangzhou city. Six official or candidate species of , including two confirmed zoonotic species, were detected with an overall prevalence of 6.4% in rodents captured herein. In addition, was the predominant host species for infection, and was the dominant species circulating in rodents in these areas. These results provide insights into the prevalence and genetic diversity of species circulating in rodents in the urban areas of Guangzhou, and also urged the surveillance of rodent-associated species in these areas.
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http://dx.doi.org/10.3389/fmicb.2022.942587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289675PMC
July 2022

In vivo study of dual functionalized mussel-derived bioactive peptides promoting 3D-printed porous Ti6Al4V scaffolds for repair of rabbit femoral defects.

J Biomater Appl 2022 Jul 20:8853282221117209. Epub 2022 Jul 20.

599923Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, China.

The 3D printed porous titanium alloy scaffolds are beneficial to enhance angiogenesis, osteoblast adhesion, and promote osseointegration. However, titanium alloys are biologically inert, which makes the bond between the implant and bone tissue weak and prone to loosening. Inspired by the natural biological marine mussels, we designed four-claw-shaped mussel-derived bioactive peptides for the decoration of porous titanium alloy scaffolds: adhesion peptide-DOPA, anchoring peptide-RGD and osteogenic-inducing peptide-BMP-2. And the bifunctionalization of 3D-printed porous titanium alloy scaffolds was evaluated in vivo in a rabbit model of bone defect with excellent promotion of osseointegration and mechanical stability. Our results show that the in vivo osseointegration ability of the modified 3D printed porous titanium alloy test piece is significantly improved, and the bifunctional polypeptide coating group E has the strongest osseointegration ability. In conclusion, our experimental design partially solves the problems of stress shielding effect and biological inertness, and provides a convenient and feasible method for the clinical application of titanium alloy implants in biomedical implant materials.
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http://dx.doi.org/10.1177/08853282221117209DOI Listing
July 2022

Anxiety, depression, and related factors in hemodialysis patients during the lockdown period of COVID- 19 in China: a multicenter study.

Psychol Health Med 2022 Jul 18:1-7. Epub 2022 Jul 18.

Department of Nephrology, The Ninth People's Hospital of Chongqing, Chongqing, China.

The outbreak of coronavirus disease 2019 (COVID-19) made a huge impact on hemodialysis (HD) patients. The aim of the study was to survey the anxiety, depression and related factors of HD patients during the lockdown and to explore the factors that affect their anxiety and depression. From February 1 to 15, 2020, an online survey was conducted in HD patients; the survey collected information on demographic data, epidemic perception, travel status and Zung's Self-rating Anxiety Scale (SAS) and Zung's Self-rating Depression Scale (SDS). The original SAS and SDS data from 96 HD patients gathered between May and August 2018 were retained as data for the control group. A total of 506 HD patients were recruited, and the prevalence of anxiety and depression was 22.33% and 45.45%, respectively. They had significantly higher SAS and SDS scores than the HD patients before the epidemic ( < 0.01; < 0.05). It was found that self-threat perception, trouble related to transportation and skipping dialysis sessions were factors related to anxiety in HD patients during the lockdown ( ˂ 0.05). Education level, trouble related to transportation and the awareness of the seriousness of the epidemic were factors related to depression ( ˂ 0.05). During the COVID-19 lockdown period, HD patients reported higher levels of psychological distress than HD patients before the pandemic. Psychological support and transport policies should be provided to them.
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http://dx.doi.org/10.1080/13548506.2022.2100917DOI Listing
July 2022

Serum lipids and risk of incident psoriasis: a prospective cohort study from the UK Biobank study and Mendelian randomization analysis.

J Invest Dermatol 2022 Jul 15. Epub 2022 Jul 15.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China 410008; Hunan Engineering Research Center of Skin Health and Disease, Changsha, China 410008; National Clinical Research Center for Geriatric Disorders (Xiangya Hospital), Changsha, China 410008; Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha, China 410078. Electronic address:

The association between dyslipidemia and psoriasis has been studied widely. However, which individual indictors of serum lipids dominate increasing the risk of incident psoriasis is still underappreciated in prospective cohorts. Based on UK Biobank, we investigate the causal relationship between four serum lipids and incident psoriasis by Cox proportional hazard model and Mendelian randomization (MR) analysis. After adjusting for covariates, HDL deficiency (<1.0 mmol/L for men, <1.3 mmol/L for women) and high triglyceride level (≥1.7 mmol/L) at baseline were associated with 16.6% and 10.6% increased risk of incident psoriasis. The effects were more pronounced in women with 16.9% and 19.7% additional risk of psoriasis, respectively. The effects in the younger group (<60 years old) and obese group in women were more pronounced either. No similar effect was observed in LDL and total cholesterol. Our subsequent MR analysis reinforced the main finding that HDL deficiency and high triglyceride cause incident psoriasis genetically. In conclusion, serum HDL/triglyceride levels predict psoriasis particularly in women, indicating a distinct role of lipids engaging in the pathogenesis of psoriasis modified by sex. More metabolic-targeted, sex-specific management of psoriasis is suggested in the future.
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http://dx.doi.org/10.1016/j.jid.2022.06.015DOI Listing
July 2022

A Ferroptosis Molecular Subtype-Related Signature for Predicting Prognosis and Response to Chemotherapy in Patients with Chronic Lymphocytic Leukemia.

Biomed Res Int 2022 6;2022:5646275. Epub 2022 Jul 6.

Department of Hematology, The Second Xiangya Hospital; Molecular Biology Research Center, Center for Medical Genetics, School of Life Sciences; Hunan Province Key Laboratory of Basic and Applied Hematology, Central South University, Changsha 410011, China.

Ferroptosis is a type of regulated cell death catalyzed by the iron-dependent accumulation of lipid hydroperoxides to lethal levels. Chronic lymphocytic leukemia (CLL) is a chronic lymphoproliferative disorder. However, the understanding of ferroptosis in CLL remains largely poor. In this study, we investigated the stratification and prognostic role of ferroptosis-related genes in CLL patients of ICGC cohort. We obtained fourteen genes with prognostic value by screening 110 ferroptosis-related genes (FRGs). Based on the expression profiles of these 14 genes, we classified CLL patients into two clusters. Most of the FRGs were highly expressed in cluster 1, and cluster 1 was associated with better overall survival (OS). Subsequently, we developed an eight-gene signature (TP63, STEAP3, NQO1, ELAVL1, PRKAA1, HELLS, FANCD2, and CDKN2A) by using LASSO analysis. This risk signature divided CLL patients into high- and low-risk groups. We used Cox regression analysis and ROC analysis demonstrated the risk signature was reliable and robust. And we validated the risk model in an external cohort (GSE22762). We also conducted enrichment analysis and genomic mutation analysis. Finally, we explored the potential effect of chemotherapy between the two risk groups. Our study contributed to understanding the role of ferroptosis in CLL and facilitated personalized and precision treatment.
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http://dx.doi.org/10.1155/2022/5646275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279058PMC
July 2022

Next-generation sequencing reveals the presence of mutations in acute lymphoblastic leukemia and aplastic anemia.

EJHaem 2021 Aug 27;2(3):508-513. Epub 2021 Jun 27.

Divison of Laboratory Medicine Hebei Yanda Lu Daopei Hospital Langfang China.

Limited studies have been described DEAD-box helicase 41 ( mutations in hematological diseases other than myeloid neoplasms. In this study, mutations were identified in 0.8% of myeloid neoplasms, 0.9% of acute lymphoblastic leukemia (ALL), and 1.0% of aplastic anemia (AA). A total of 15 causal variants in 14 patients were detected; seven of which have not been reported previously. In myeloid neoplasms, the median age of patients with germline missense was lower than that of germline nonsense mutations. In ALL, the characteristics of mutation were distinct. This study first reported mutations in ALL and AA, expanding its mutation and phenotypic spectrum.
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http://dx.doi.org/10.1002/jha2.256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176149PMC
August 2021
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