Publications by authors named "Hong Lin"

824 Publications

Atomic Chromium Coordinated Graphitic Carbon Nitride for Bioinspired Antibiofouling in Seawater.

Adv Sci (Weinh) 2022 Jan 20:e2105346. Epub 2022 Jan 20.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, P. R. China.

Artificial nanozymes exerting enzyme functionality are recognized as promising alternatives of natural enzymes in biomimetic chemistry. Natural haloperoxidases that utilize hydrogen peroxide (H O ) to catalytically convert halide into strong biocidal hypohalous acid hold great promise for thwarting biofouling, while their practical application remains highly questionable as instability of natural enzymes and inadequate H O . Herein a semiconducting nanozyme consisting of chromium single atoms coordinated on carbon nitride (Cr-SA-CN) that performs bifunctional roles of nonsacrificial H O photosynthesis and haloperoxidase-mimicking activity for antibiofouling is constructed. Such nanozyme is capable of generating H O from water and O upon visible-light illumination, and then sustainably self-supplying H O for haloperoxidase-mimicking reaction in a sequential manner. This dual-activity Cr-SA-CN overcomes H O dilemma and yields hypobromous acid continuously, inducing remarkable bactericidal capability. When used as an eco-friendly coating additive, it is successfully demonstrated that Cr-SA-CN enables an inert surface against marine biofouling. Thereby, this study not only illustrates an attractive strategy for antibiofouling but also opens an avenue to construct valuable nanoplatform with multifunctionality for future applications.
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http://dx.doi.org/10.1002/advs.202105346DOI Listing
January 2022

Quorum Sensing Promotes Phage Infection in Pseudomonas aeruginosa PAO1.

mBio 2022 Jan 18:e0317421. Epub 2022 Jan 18.

Food Safety Laboratory, College of Food Science and Engineering, Ocean University of Chinagrid.4422.0, Qingdao, China.

Quorum sensing (QS) is used to coordinate social behaviors, such as virulence and biofilm formation, across bacterial populations. However, the role of QS in regulating phage-bacterium interactions remains unclear. Preventing phage recognition and adsorption are the first steps of bacterial defense against phages; however, both phage recognition and adsorption are a prerequisite for the successful application of phage therapy. In the present study, we report that QS upregulated the expression of phage receptors, thus increasing phage adsorption and infection rates in Pseudomonas aeruginosa. In P. aeruginosa PAO1, we found that QS, instead of QS, upregulated the expression of for lipopolysaccharide synthesis. Lipopolysaccharides act as the receptor of the phage vB_Pae_QDWS. This QS-mediated phage susceptibility is a dynamic process, depending on host cell density. Our data suggest that inhibiting QS may reduce the therapeutic efficacy of phages. Phage resistance is a major limitation of phage therapy, and understanding the mechanisms by which bacteria block phage infection is critical for the successful application of phage therapy. In the present study, we found that Pseudomonas aeruginosa PAO1 uses QS to promote phage infection by upregulating the expression of , which is necessary for the synthesis of phage receptor lipopolysaccharides. In contrast to the results of previous reports, we showed that QS increases the efficacy of phage-mediated bacterial killing. Since QS upregulates the expression of virulence factors and promotes biofilm development, which are positively correlated with lipopolysaccharide production in P. aeruginosa, increased phage susceptibility is a novel QS-mediated trade-off. QS inhibition may increase the efficacy of antibiotic treatment, but it will reduce the effectiveness of phage therapy.
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http://dx.doi.org/10.1128/mbio.03174-21DOI Listing
January 2022

Hypertension Defined by 2017 ACC/AHA Guideline, Ideal Cardiovascular Health Metrics, and Risk of Cardiovascular Disease: A Nationwide Prospective Cohort Study.

Lancet Reg Health West Pac 2022 Mar 8;20:100350. Epub 2022 Jan 8.

The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: The updated definition of hypertension by the American College of Cardiology (ACC) and the American Heart Association (AHA) is an important paradigm shift and has lead to extensive discussion. We aimed to examine the association between the updated blood pressure (BP) categories and the risk of cardiovascular diseases (CVDs) with potential modifications from other cardiovascular health metrics (CVHMs).

Methods: This prospective study included 91,204 participants ≥40 years recruited from 20 community sites across mainland China. Participants were followed up during 2010-2016 for CVD events including nonfatal myocardial infarction, stroke, heart failure, and cardiovascular death. BP categories were defined according to the 2017 ACC/AHA guideline and CVHMs included smoking, physical activity, diet, body-mass index, total cholesterol, and fasting glucose.

Findings: Overall, 1,985 major CVD events occurred during a mean follow-up of 3.7 years. Having more ideal CVHMs significantly reduced the risk of CVD events in both stage 1 and stage 2 hypertension. Compared with participants without hypertension, participants having ≥4 ideal CVHMs were no longer associated with an increased CVD risk in stage 1 hypertension (HR=1·04; 95% CI=0·83-1·31), but less so in stage 2 hypertension (HR=1·90, 95% CI=1·70-2·13). Such pattern of association was more evident in participants aged <60 years ( for interaction <0·05).

Interpretation: Stage 1 hypertension defined by the ACC/AHA identifies individuals at increased CVD risk, which can be attenuated by achieving more preferable cardiovascular health, especially in adults aged <60 years.
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http://dx.doi.org/10.1016/j.lanwpc.2021.100350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749453PMC
March 2022

Expression of a phage-encoded Gp21 protein protects against phage infection.

J Virol 2022 Jan 12:JVI0176921. Epub 2022 Jan 12.

Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.

There is a continuously expanding gap between predicted phage gene sequences and their corresponding functions, which largely hampered the development of phage therapy. Previous studies reported several phage proteins that could interfere with the intracellular processes of the host to obtain efficient infection. But few phage proteins that protect host against phage infection has been identified and characterized in detail. Here, we isolate a phage vB_Pae_QDWS capable of infecting PAO1, and report its encoded Gp21 protein protects PAO1 against phage infection. Expressing of Gp21 regulate bacterial quorum sensing with an inhibitory effect in low cell density and activation effect in high cell density. By testing the TFPs-mediated twitching motility and transmission electron microscopy analysis, Gp21 was found decreased the pilus synthesis. Further constructing the TFPs synthesis gene mutant and performing adsorption and phage resistance assay, we demonstrated Gp21 protein could block phage infection via decreasing the TFPs-mediated phage adsorption. Gp21 is a novel protein that inhibit phage efficacy against bacteria. The study deepens our understanding of phage-host interactions. The majority of the annotated phage genes are currently deposited as "hypothetical protein" with unknown function. Researches revealed that some phage proteins serve to inhibit or redirect the host intracellular processes for phage infection. Differently, we report a phage encoded protein Gp21 that protect the host against phage infection. The pathways that Gp21 involved in anti-phage defense in PAO1 are interfering with quorum sensing and decreasing the type IV pilus-mediated phage adsorption. Gp21 is a novel protein with a low sequence homology with other reported twitching inhibitory proteins. As a lytic phage derived protein, Gp21 expression protects PAO1 from reinfection by phage vB_Pae_QDWS, which may explain the well-known pseudolysogeny caused by virulent phages. Our discoveries provide valuable new insight into the phage-host evolutionary dynamics.
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http://dx.doi.org/10.1128/JVI.01769-21DOI Listing
January 2022

Consensus of Linear Multivariable Discrete-Time Multiagent Systems: Differential Privacy Perspective.

IEEE Trans Cybern 2022 Jan 11;PP. Epub 2022 Jan 11.

Differential privacy, which has been widely applied in industries, is a privacy mechanism effective in preventing malicious entities from breaching the privacy of an individual participant. It is usually achieved by adding random variables in the data. This article investigates a class of multivariable discrete-time multiagent systems with ε-differential privacy preserved. A novel information-masking mechanism is proposed, in which the information of each state transmitted to different neighbors is obscured by adding independent random noises. Then, the mean-square consensus conditions, and the upper bound and lower bound of the convergence rate are obtained. Moreover, the conditions for the convergence rate reaching its upper bound are established. The results can be applied to the average mean-square consensus. In addition, a necessary and sufficient condition is presented under which agents can preserve the dynamics of agents ε-differentially private at any time instant.
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http://dx.doi.org/10.1109/TCYB.2021.3135933DOI Listing
January 2022

Gene is Associated With Sex Determination in .

Front Genet 2021 23;12:727260. Epub 2021 Dec 23.

College of Horticulture, Shenyang Agricultural University, Shenyang, China.

In the past decade, progress has been made in sex determination mechanism in . However, genes responsible for sexual differentiation and its mechanism in remain unknown. Here, we identify a sex determination candidate gene coding adenine phosphoribosyl transferase 3 () in . Cloning and sequencing of the gene allowed us to develop a molecular marker able to discriminate female individuals from males or hermaphrodites based on a 22-bp InDel. Gene expression and endogenous cytokinin content analysis revealed that the gene is involved in sex determination or, to be precise, in female organ differentiation, through regulating cytokinin metabolism in . This study enlarged the understanding of sex determination mechanism in the genus , and the sex marker could be used as a helpful tool for sexual identification in breeding programs as well as in investiation and collection of germplasms.
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http://dx.doi.org/10.3389/fgene.2021.727260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733387PMC
December 2021

Toward Hydrogen-Free and Dendrite-Free Aqueous Zinc Batteries: Formation of Zincophilic Protective Layer on Zn Anodes.

Adv Sci (Weinh) 2022 Jan 6:e2104866. Epub 2022 Jan 6.

School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, P. R. China.

Rechargeable aqueous Zn-ion batteries (ZIBs) are regarded as one of the most promising devices for the next-generation energy storage system. However, the uncontrolled dendrite growth on Zn metal anodes and the side hydrogen evolution reaction, which has not yet been well considered, hinder the practical application of these batteries. Herein, a uniform and robust metallic Sb protective layer is designed based on the theoretic calculation and decorated on Zn plate via in situ replacement reaction. Compared with the bare Zn plate, the as-prepared [email protected] electrode provides abundant zincophilic sites for Zn nucleation, and homogenizes the electric field around the Zn anode surface, both of which promote the uniform Zn deposition to achieve a dendrite-free morphology. Moreover, the Gibbs free energy (∆G ) calculation and in situ characterization demonstrate that hydrogen evolution reaction can be effectively suppressed by the Sb layer. Consequently, Sb-modified Zn anodes exhibit an ultralow voltage hysteresis of 34 mV and achieve excellent cycling stability over 1000 h with hydrogen- and dendrite-free behaviors. This work provides a facile and effective strategy to suppress both hydrogen evolution reaction and dendrite growth.
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http://dx.doi.org/10.1002/advs.202104866DOI Listing
January 2022

Immunological Cross-Reactivity Involving Mollusc Species and Mite-Mollusc and Cross-Reactive Allergen PM Are Risk Factors of Mollusc Allergy.

J Agric Food Chem 2022 Jan 3;70(1):360-372. Epub 2022 Jan 3.

College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province 266003, P. R. China.

Marine molluscs are seafood consumed worldwide and could cause food allergies, while investigation on their sensitizing components and cross-reactivity seems to be rare. Furthermore, allergy to mites may result in anaphylaxis in mollusc-allergic individuals owing to their cross-reactivity. The aim of the study was to identify cross-reactive allergens and investigate the cross-reactivity between different mollusc groups and mite-mollusc. The extracted mollusc and dust mite proteins were separated by SDS-PAGE, and IgE-binding components were recognized by immunoblotting with sera from patients sensitized to mollusc and mite. Cross-reactivity of different mollusc groups and mite-mollusc was assessed using ELISA and inhibition ELISA. The results of the immune detection, ELISA, and inhibition ELISA indicated that different mollusc groups and mite-mollusc showed varying degrees of cross-reactivity. The most frequently recognized cross-reactive protein was paramyosin from different mollusc groups and dust mite, while cross-reactive allergen paramyosin in the mite extract was identified and evaluated by MS and Allermatch, respectively. Inhibition ELISA studies also revealed that paramyosin played an important role in molluscan and mite-molluscan cross-reactivity. These findings contribute to a better understanding of the cross-reactivity involving mollusc species and mite-mollusc, which can be used to assist in the diagnosis and treatment of mite- and mollusc-allergic disorders.
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http://dx.doi.org/10.1021/acs.jafc.1c05421DOI Listing
January 2022

New clusters of serum electrolytes aid in stratification of diabetes and metabolic risk.

J Diabetes 2021 Dec 28. Epub 2021 Dec 28.

Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Serum electrolytes were found to associate with type 2 diabetes. Our study aimed to stratify nondiabetes by clusters based on multiple serum electrolytes and evaluate their associations with risk of developing diabetes and longitudinal changes in glucose and lipid metabolic traits.

Methods: We performed a data-driven cluster analysis in 4937 nondiabetes individuals aged ≥40 years at baseline from a cohort follow-up for an average of 4.4 years. Cluster analysis was based on seven commonly measured serum electrolytes (iron, chlorine, magnesium, sodium, potassium, calcium, and phosphorus) by using the k-means method.

Results: A total of 4937 nondiabetes individuals were classified into three distinct clusters, with 1635 (33.1%) assigned to Cluster A, 1490 (30.2%) to Cluster B, and 1812 (36.7%) to Cluster C. Individuals in Cluster A had higher serum chlorine, were older, and more were women. Individuals in Cluster B had higher serum iron and body mass index (BMI). Individuals in Cluster C had higher serum phosphorus, were younger, and had lower BMI. Cluster B had 1.41-fold higher risk of developing diabetes and Cluster C's risk was 1.33-fold higher compared with Cluster A. Over an average follow-up of 4.4 years, Cluster A showed a moderate and stable BMI, Cluster B showed an accelerated deterioration in glucose metabolism, and Cluster C showed the most sharply increased serum low-density lipoprotein cholesterol level.

Conclusions: Clusters based on seven common serum electrolytes differed in diabetes risk and progression of glucose and lipid metabolic traits. Serum electrolytes clusters could provide a powerful tool to differentiate individuals into different risk stratification for developing type 2 diabetes.
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http://dx.doi.org/10.1111/1753-0407.13244DOI Listing
December 2021

The Effect of Daily Teriparatide versus One-Time Annually Zoledronic Acid Administration After Transforaminal Lumbar Interbody Fusion in Osteoporotic Patients.

Clin Interv Aging 2021 5;16:1789-1799. Epub 2021 Oct 5.

Department of Orthopedics, Zhongshan Hospital, Fudan University, Shanghai, 200032, People's Republic of China.

Purpose: The research aimed to compare the therapeutic effect of teriparatide (TPTD) and zoledronic acid (ZOL) therapy on bone formation and spinal fusion in patients with osteoporosis (OP) who underwent transforaminal lumbar interbody fusion (TLIF).

Methods: On the basis of different anti-OP treatment options, the TPTD group was treated daily with TPTD (20 μg. ih. qd) for at least 6 months, while the ZOL group was treated with a single dose of ZOL (5 mg. ivgtt. st) postoperatively. The visual analogue scale (VAS), Oswestry Disability Index (ODI), bone mineral density (BMD), and concentration of bone turnover markers before, 6, and 12 months after surgery were evaluated. X-ray and three-dimensional computed tomography scans were performed at 6 and 12 months postoperatively to assess interbody fusion.

Results: The number of patients in the TPTD and ZOL groups was 29 and 38 patients, respectively. The VAS and ODI scores in both groups were significantly reduced at 6 and 12 months after TLIF. Compared with that of baseline, the lumbar spine BMD of TPTD patients increased significantly from 0.716±0.137 g/cm to 0.745±0.124 g/cm and 0.795±0.123 g/cm at 6 and 12 months, respectively, and was significantly higher than that of the ZOL group at 12 months (0.720±0.128 g/cm). The bone formation marker, P1NP, in the TPTD group increased significantly (145.48±66.64 ng/mL and 119.55±88.27 ng/mL) compared with baseline (44.67±25.15 ng/mL) and in the ZOL group (28.82±19.76 ng/mL and 29.94±20.67 ng/mL) at 6 and 12 months, respectively. The fusion rates in the TPTD and ZOL groups were 57% and 45% at 6 months, without statistical significance. However, TPTD had a more statistically significant positive influence on fusion rate than ZOL at 12 months (86% vs 70%).

Conclusion: TPTD was more efficient than ZOL in bone formation and spinal fusion in OP patients who underwent TLIF.
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http://dx.doi.org/10.2147/CIA.S333207DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678629PMC
December 2021

Analysis of Hybrid Facies, Hybrid Sequence, and Its Controlling Factors of the Devonian Yangmaba Formation in the Northwestern Sichuan Basin, Southern China.

ACS Omega 2021 Dec 1;6(49):33627-33638. Epub 2021 Dec 1.

Institute of Geological Sciences of Jiangxi Province, Nanchang, Jiangxi 330000, China.

Abundant hybrid sediments composed of clastic rocks and carbonate rocks were deposited in the Devonian Yangmaba Formation in the northwestern of Sichuan Basin. Based on the measurement of the Ganxi section in detail, combined with the observation of the 30 slices, the hybrid facies, and hybrid sequence, the hybrid deposit mechanism and its controlling factors of the Yangmaba Formation were analyzed. It shows that the hybrid facies consists of the hybrid shore at the lower and clastic hybrid shelf and carbonate hybrid shelf deposits at the middle-upper of the Yangmaba Formation. The hybrid sequence, which can be divided into four sedimentary system tracts: the shelf-margin systems tract (SMST), the transgressive system tract (TST), the early highstand systems tract (EHST), and the late highstand systems tract (LHST), was developed in the Yangmaba Formation. There are three hybrid mechanisms including punctuated mixing, facies mixing, and in situ mixing, and the first two are the main types in the Yangmaba Formation. The punctuated mixing and in situ mixing are the main hybrid mechanisms of hybrid shore and clastic hybrid shelf deposits, and the facies mixing is the main hybrid mechanism of clastic hybrid shelf and carbonate hybrid shelf deposits. The hybrid mechanisms are different among the system tracts: the punctuated mixing is the main mixing manner in the SMST and LHST and the facies mixing developed in the TST and EHST. Storm action is the most important controlling factor of punctuated mixing of the Yangmaba Formation. Relative sea level change, carbonate productivity or the rate of terrestrial clastic supply, and climate change are factors that control and affect hybrid deposits. SMST and HST are mainly related to changes in the relative sea level, while TST is controlled by sedimentary source recharge, and climate change affects the hybrid action of system tracts to varying degrees.
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http://dx.doi.org/10.1021/acsomega.1c04493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675035PMC
December 2021

Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells.

Front Bioeng Biotechnol 2021 30;9:706423. Epub 2021 Nov 30.

Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

In consideration of improving the interface problems of poly-L-lactic acid (PLLA) that hindered biomedical use, surface coatings have been explored as an appealing strategy in establishing a multi-functional coating for osteogenesis. Though the layer-by-layer (LBL) coating developed, a few studies have applied double-crosslinked hydrogels in this technique. In this research, we established a bilayer coating with double-crosslinked hydrogels [alginate-gelatin methacrylate (GelMA)] containing bone morphogenic protein (BMP)-2 [alginate-GelMA/hydroxyapatite (HA)/BMP-2], which displayed great biocompatibility and osteogenesis. The characterization of the coating showed improved properties and enhanced wettability of the native PLLA. To evaluate the biosafety and inductive ability of osteogenesis, the behavior (viability, adherence, and proliferation) and morphology of human bone mesenchymal stem cells (hBMSCs) on the bilayer coatings were tested by multiple exams. The satisfactory function of osteogenesis was verified in bilayer coatings. We found the best ratios between GelMA and alginate for biological applications. The Alg70-Gel30 and Alg50-Gel50 groups facilitated the osteogenic transformation of hBMSCs. In brief, alginate-GelMA/HA/BMP-2 could increase the hBMSCs' early transformation of osteoblast lineage and promote the osteogenesis of bone defect, especially the outer hydrogel layer such as Alg70-Gel30 and Alg50-Gel50.
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http://dx.doi.org/10.3389/fbioe.2021.706423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670089PMC
November 2021

Associations of body shapes with insulin resistance and cardiometabolic risk in middle-aged and elderly Chinese.

Nutr Metab (Lond) 2021 Dec 7;18(1):103. Epub 2021 Dec 7.

Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, 200025, Shanghai, China.

Background: We aimed to define refined body shapes by using multiple anthropometric traits that represent fat distribution, and evaluate their associations with risk of insulin resistance (IR) and cardiometabolic disorders in a Chinese population.

Methods: We performed a cross-sectional analysis in 6570 community-based participants aged ≥ 40 years. Four body circumferences (neck, waist, hip, and thigh) and their ratios were put simultaneously into an open-source Waikato Environment for Knowledge Analysis platform to select the worthiest indicators in determining IR. The ratio of the top 3 fat distribution indicators was used to define the refined body shapes.

Results: We defined 8 distinct body shapes based on sex-specific combinations of waist-to-hip ratio (WHR), waist-to-thigh ratio (WTR), and waist-to-neck ratio (WNR), which differed in participants' distribution and risk of IR and related cardiometabolic disorders. In women, as compared to the low WHR-low WTR-low WNR shape, all body shapes were significantly associated with IR and related cardiometabolic disorders; while in men, the low WHR-high WTR-high WNR shape and the higher WHR related shapes were significantly associated with IR and related cardiometabolic disorders. Stratified by WHR, the results were consistent in women; however, no significant associations were detected in men.

Conclusions: We defined 8 distinct body shapes by taking WHR, WTR, and WNR, simultaneously into account, which differed in association with the risk of IR and related cardiometabolic disorders in women. This study suggests that body shapes defined by multiple anthropometric traits could provide a useful, convenient, and easily available method for identifying cardiometabolic risk.
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http://dx.doi.org/10.1186/s12986-021-00629-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650554PMC
December 2021

Jintiange Capsules Ameliorate Osteoarthritis by Modulating Subchondral Bone Remodeling and Protecting Cartilage Against Degradation.

Front Pharmacol 2021 11;12:762543. Epub 2021 Nov 11.

Department of Orthopedics, Zhongshan Hospital, Fudan University, Shanghai, China.

Osteoarthritis (OA) is the most prevalent joint disease worldwide, making it a major cause of pain and disability. Identified as a chronic and progressive disease, effective treatment at the early stages of OA has become critical to its management. Jintiange (Jtg) capsules are a traditional Chinese medicine produced from multiple organic components of various animal bones and routinely used to treat osteoporosis in China. However, the effect of Jtg on subchondral bone and cartilage degeneration in OA remains unknown. The purpose of the present study was to investigate the biomolecular role and underlying mechanisms of Jtg in OA progression. Herein, we found that Jtg inhibited receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation and it functions through the NF-κB signaling pathway. Jtg also inhibited chondrocyte apoptosis via reducing the reactive oxygen species concentration in these cells. Moreover, evaluation revealed that Jtg significantly attenuates subchondral bone remodeling and cartilage destruction in anterior cruciate ligament transection (ACLT) mouse models. Taken together, our data demonstrate that Jtg inhibits osteoclast differentiation in subchondral bone and chondrocyte apoptosis in cartilage, supporting its potential therapeutic value for treating OA.
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http://dx.doi.org/10.3389/fphar.2021.762543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631927PMC
November 2021

A Systematic Investigation Unveils High Coinfection Status of Porcine Parvovirus Types 1 through 7 in China from 2016 to 2020.

Microbiol Spectr 2021 12 1;9(3):e0129421. Epub 2021 Dec 1.

College of Veterinary Medicine, Yangzhou Universitygrid.268415.c, Yangzhou, Jiangsu, China.

Porcine parvovirus genotype 1 (PPV1) causes reproductive disorder in swine and is prevalent in China. Recently, six new genotypes of PPVs (PPV2 through PPV7) have also been detected in Chinese swine herds. However, the coinfection status of all these seven genotypes of PPVs (PPV1-7) in China was not clarified yet. In this study, we developed a panel of PPV1-7 PCR assays with satisfied specificity, sensitivity and reproducibility and then applied to the detection of PPV1-7 in 435 clinical samples collected from eight provinces of China in 2016-2020. A total of 55.40% samples (241 out of 435) were PPV positive, while PPV2 and PPV3 (both 22.53%) belonging to the genus of were the most prevalent genotypes. Noticeably, PPV1-7 strains were more prevalent in nursery and finishing pigs than in suckling pigs. In addition, coinfection could be detected in all eight provinces and 27.36% (119/435) samples were coinfected with two to five genotypes of PPVs. Meanwhile, the coinfection of PPVs with PCV2 was 22.30% (97/435). Twenty complete genomes of representative PPV1-7 were determined, and phylogenetic analysis confirmed the genotyping results by sequence comparisons and PCR assays. Remarkably, the PPV7 HBTZ20180519-152 strain from domestic pig was recombined from parental JX15-like and JX38-like isolates from wild boars. Selective pressure analysis based on VP2 sequences of PPV1-7 showed that they were predominantly under negative selection, while few positive selection sites could be detected in VP2 of PPV7. Overall, this systematic investigation unveils high prevalence and coinfection of PPV1-7 in China from 2016 to 2020. Porcine parvoviruses (PPVs) are prevalent in China associating with reproductive failure in swine. The coinfection of seven genotypes of PPVs (PPV1-7) might have synergistic effects on PPV1 associated SMEDI syndrome. However, the coinfection status of PPV1-7 in China is not clear yet. This study showed that PPV1-7 strains are highly prevalent (55.40%) in China and mainly in nursery and finishing pigs in recent years. In addition, the coinfections of different genotypes of PPVs (27.36%) and PPVs with PCV2 (22.30%) are common. Geographic analysis indicated that different genotypes of PPVs are widely cocirculating in China. Intriguingly, a PPV7 strain from the domestic pig was detected as a recombinant from two wild boar isolates. Selective pressure analyses showed that PPV1-7 are mainly under purifying selection. Our findings provide the first systematic investigation on the prevalence, coinfection, and evolution of PPV1 through PPV7 in Chinese swineherds from 2016 to 2020.
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http://dx.doi.org/10.1128/Spectrum.01294-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635132PMC
December 2021

Microstructurally Tailored Thin β-Ag Se Films toward Commercial Flexible Thermoelectrics.

Adv Mater 2021 Nov 26:e2104786. Epub 2021 Nov 26.

Key Laboratory for Micro-Nano Energy Storage and Conversion Materials of Henan Province, College of Advanced Materials and Energy, Institute of Surface Micro and Nano Materials, Xuchang University, No. 88 Bayi Road, Xuchang City, Henan, 461000, P. R. China.

Aiming to overcome both the structural and commercial limitations of flexible thermoelectric power generators, an efficient room-temperature aqueous selenization reaction that can be completed in air within less than 1 min, to directly fabricate thin β-Ag Se films consisting of perfectly crystalline and large columnar grains with both in-plane randomness and out-of-plane [201] preferred orientation, is designed. A high power factor (PF) of 2590 ± 414 µW m K and a figure-of-merit (zT) of 1.2 ± 0.42 are obtained from a sample with a thickness of ≈1 µm. The maximum output power density of the best 4-leg thermoelectric generator sample reach 27.6 ± 1.95 and 124 ± 8.78 W m at room temperature with 30 and 60 K temperature differences, respectively, which may be useful in future flexible thermoelectric devices.
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http://dx.doi.org/10.1002/adma.202104786DOI Listing
November 2021

The effect of chlorophyll on the enzyme-linked immunosorbent assay (ELISA) of procymidone in vegetables and the way to overcome the matrix interference.

J Sci Food Agric 2021 Nov 25. Epub 2021 Nov 25.

Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, China.

Background: There is now an increasing demand for the immunoassay of procymidone residue in foodstuffs. However, the matrix interference could significantly affect the analysis. Till now there is no detailed information on the source of the interference and the mechanism involved, which greatly limits the real application of these techniques.

Results: Significant matrix effect was observed in the enzyme-linked immunosorbent assay (ELISA) of procymidone in negative vegetable samples (leek, broccoli and cucumber). By the investigation with both vegetable extracts and standard solutions, the chlorophyll was confirmed as an important source of the matrix effect. Therefore, a new strategy was proposed for the pretreatment based on the exploitation of 5-sulfosalicylic acid. It was demonstrated to effectively eliminate chlorophyll and exhibited little effect on procymidone and the competitive indirect ELISA (ci-ELISA) performance. The established technique was validated with different vegetables. With the spiking concentration of procymidone investigated, the recovery rate of ci-ELISA was 71.52-120.37%, and the relative standard deviation was 4.05-17.61%.

Conclusion: Chlorophyll was for the first time illuminated as an important source of matrix interference to the immunoassay of procymidone in vegetables. A new pretreatment based on 5-sulfosalicylic acid was established to remove chlorophyll and therefore eliminate the matrix effect. Validated with different vegetable samples, the new technique was demonstrated much better efficiency in comparison to conventional methods, which indicated its promising application for the development of immunoassays of herb-origin samples. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11686DOI Listing
November 2021

Metabolomics study reveals systematic metabolic dysregulation and early detection markers associated with incident pancreatic cancer.

Int J Cancer 2021 Nov 18. Epub 2021 Nov 18.

Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Biomarkers for early detection of pancreatic cancer are in urgent need. To explore systematic circulating metabolites unbalance and identify potential biomarkers for pancreatic cancer in prospective Chinese cohorts, we conducted an untargeted metabolomics study in subjects with incident pancreatic cancer and matched controls (n = 192) from the China Cardiometabolic Disease and Cancer Cohort (4C) Study. We characterized 998 metabolites in baseline serum and calculated 156 product-to-precursor ratios based on the KEGG database. The identified metabolic profiling revealed systematic metabolic network disorders before pancreatic cancer diagnosis. Forty-Five metabolites or product-to-precursor ratios showed significant associations with pancreatic cancer (P < .05 and FDR < 0.1), revealing abnormal metabolism of amino acids (especially alanine, aspartate and glutamate), lipids (especially steroid hormones), vitamins, nucleotides and peptides. A novel metabolite panel containing aspartate/alanine (OR [95% CI]: 1.97 [1.31-2.94]), androstenediol monosulfate (0.69 [0.49-0.97]) and glycylvaline (1.68 [1.04-2.70]) was significantly associated with risk of pancreatic cancer. Area under the receiver operating characteristic curves (AUCs) was improved from 0.573 (reference model of CA 19-9) to 0.721. The novel metabolite panel was validated in an independent cohort with AUC improved from 0.529 to 0.661. These biomarkers may have a potential value in early detection of pancreatic cancer.
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http://dx.doi.org/10.1002/ijc.33877DOI Listing
November 2021

Intervention of 4% salmon phospholipid on metabolic syndrome in mice based on colonic lipidomics analysis.

J Sci Food Agric 2021 Nov 14. Epub 2021 Nov 14.

Team of Neonatal and Infant Development, Health and Nutrition (NDHN), School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, PR China.

Background: The incidence of metabolic syndrome (MetS) is increasing, and n-3 polyunsaturated fatty acids (PUFAs) in salmon (Oncorhynchus) phospholipids can effectively reduce the risk of MetS.

Results: Under the intervention of 4% salmon phospholipid, the levels of fasting blood glucose (FBG), insulin, monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were significantly reduced in the plasma of MetS mice, whereas adiponectin was significantly increased. By screening, we found that the 18 differential metabolites, consisting of seven triglycerides (TGs), six diglycerides (DGs), one phosphatidylethanolamine (PE), three sphingomyelins (SMs) and one eicosanoid, could be the key differential metabolites, and two metabolic pathways were significantly affected: glycerolipid metabolism and glycerophospholipid metabolism.

Conclusion: 4% salmon phospholipids could affect MetS by inhibiting insulin resistance, reducing inflammatory factors and promoting the synthesis of PE, yet the mechanism required further study. Our results could help in the treatment of MetS. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11649DOI Listing
November 2021

Development of a sensitive sandwich-ELISA assay for reliable detection of fish residues in foods.

Anal Biochem 2021 12 4;635:114448. Epub 2021 Nov 4.

College of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao, Shandong Province, 266003, PR China. Electronic address:

A new sandwich-type Enzyme-Linked Immunosorbent Assay (ELISA) method was developed based on goat IgG as capturing antibody and rabbit IgG as detecting antibody targeting soluble antigenic fish proteins in foods as detection targets. The assay has provided a relatively lower limit of quantitation (LoQ) for fish proteins with LoQ 0.5 ng/ml and appears highly sensitive. The analysis of 24 different substances, both raw and boiled, revealed no cross-reactivity above the cut-off point of the limit of quantitation. Recoveries of the SB spiked food matrixes were in the range of 83-131%. Assay precision testing proved that repeatability (<5%) and reproducibility (<11%) had an acceptable level of variation. The sandwich ELISA was capable of detecting all tested commercially important fish. As a potential analytical tool, the newly developed immunoenzymatic method is suitable for detecting undeclared fish residues in real food samples available in the market, thereby will help to reduce the incidents of fish allergies.
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http://dx.doi.org/10.1016/j.ab.2021.114448DOI Listing
December 2021

Algae, shrimp grazing, and fecal pellets synergistically increase microbial activity and enhance N immobilization during Typha angustifolia leaf litter decomposition.

Environ Sci Pollut Res Int 2021 Oct 22. Epub 2021 Oct 22.

School of Life Sciences, Nanjing University, Nanjing, 210023, People's Republic of China.

Algae play an important role in ecological processes of aquatic ecosystems. Understanding the interactive effects of algae with invertebrates in litter decomposition is important for predicting the effects of global change on aquatic ecosystems. We manipulated Typha angustifolia litter to control exposure to shrimp fecal pellets and/or grazing, and the green alga Chlorella vulgaris were added to test their interactive effects on T. angustifolia litter decomposition. Our results showed that algae largely shortened microbial conditioning time and improved litter palatability (increasing litter quality), resulting in greater decomposition and higher fecal pellet production. Fecal pellets enhanced grazing effects on decomposition by increasing litter ash content. The effects of algae and especially fecal pellets on decomposition were dependent on or mediated by grazing. Without grazing, algae slightly promoted decomposition and marginally offset the negative effect of fecal pellets on litter decomposition. Shrimp grazing dramatically decreased microbial activity (extracellular enzyme activity and microbial respiration) at microbial conditioning stage while enhanced microbial activity after 84 days especially with both algae and fecal pellets present. Algae significantly upregulated N- and P-acquiring and slightly downregulated C-acquiring enzyme activity. Fecal pellets significantly depressed recalcitrant C-decomposition enzyme activity. Nevertheless, the three factors synergistically and significantly increased C loss and most enzyme activities, microbial respiration, and N immobilization, resulting in the decrease of litter C:N. Our results reveal the synergistic action of different trophic levels (autotrophs, heterotrophs, and primary consumers) in the complicated nutrient pathways of litter decomposition and provide support for predicting the effects of global changes (e.g., N deposition and CO enrichment), which have dramatically effects on alga dynamics and on ecological processes in aquatic ecosystems.
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http://dx.doi.org/10.1007/s11356-021-16848-1DOI Listing
October 2021

Health-Related Perceptions of Older Adults/Patients with Degenerative Lumbar Diseases (ODLs) are associated with their Quality of Life: a Mixed-Methods Study.

Patient Prefer Adherence 2021 8;15:2303-2311. Epub 2021 Oct 8.

Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.

Purpose: This study aimed to elucidate the quality of life of older adults/patients with degenerative lumbar diseases (ODLs) and analyse its association with some of their health-related perceptions.

Materials And Methods: This mixed-methods study consisted of a questionnaire survey and an in-depth interview, which was designed within this study. ODLs were recruited from January 12, 2017 to June 27, 2018. The independent sample -test and grounded theory coding method were employed for data analysis.

Results: Of the 125 participants who returned valid questionnaires, 18 were included in the in-depth interviews. ODLs' quality of life was associated with the following health-related perceptions: "life barriers", "subjective health status", and "treatment outcomes" across the domains of physiology, psychology, social relations, and environment.

Conclusion: Our findings indicate that ODLs' quality of life is associated with their health-related perceptions. Thus, to improve older adults' quality of life, more attention should be paid to enhancing non-medical factors such as their health-related perceptions.
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http://dx.doi.org/10.2147/PPA.S328532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8518139PMC
October 2021

SIRT3 protects bovine mammary epithelial cells from heat stress damage by activating the AMPK signaling pathway.

Cell Death Discov 2021 Oct 21;7(1):304. Epub 2021 Oct 21.

Key Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, China.

With global warming, heat stress has become an important challenge for the global dairy industry. Sirtuin 3 (SIRT3), an important mitochondrial NAD+dependent decarboxylase and a major regulator of cellular energy metabolism and antioxidant defense, is integral to maintaining normal mitochondrial function. The aim of this study was to assess the protective effect of SIRT3 on damage to bovine mammary epithelial cells (BMECs) induced by heat stress and to explore its potential mechanism. Our results indicate that SIRT3 is significantly downregulated in heat-stressed mammary tissue and high-temperature-treated BMECs. SIRT3 knockdown significantly increased the expression of HSP70, Bax, and cleaved-caspase 3 and inhibited the production of antioxidases, thus promoting ROS production and cell apoptosis in BMECs. In addition, SIRT3 knockdown can aggravate mitochondrial damage by mediating the expression of genes related to mitochondrial fission and fusion, including dynamin-related protein 1, mitochondrial fission 1 protein, and mitochondrial fusion proteins 1and 2. In addition, SIRT3 knockdown substantially decreased AMPK phosphorylation in BMECs. In contrast, SIRT3 overexpression in high-temperature treatment had the opposite effect to SIRT3 knockdown in BMECs. SIRT3 overexpression reduced mitochondrial damage and weakened the oxidative stress response of BMECs induced by heat stress and promoted the phosphorylation of AMPK. Taken together, our results indicate that SIRT3 can protect BMECs from heat stress damage through the AMPK signaling pathway. Therefore, the reduction of oxidative stress by SIRT3 may be the primary molecular mechanism underlying resistance to heat stress in summer cows.
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http://dx.doi.org/10.1038/s41420-021-00695-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531291PMC
October 2021

Regulatory roles of phytochemicals on circular RNAs in cancer and other chronic diseases.

Pharmacol Res 2021 Dec 12;174:105936. Epub 2021 Oct 12.

Department of Pharmacy, Sichuan Cancer Hospital & Institution, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. Electronic address:

As novel non-coding RNAs (ncRNAs), circular RNAs (circRNAs) play an essential role in the pathogenesis of many chronic diseases, and the regulation of these functional molecules has become a research hotspot gradually. Within the past decade, phytochemicals were reported to regulate the expression of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in various chronic diseases, and more recently, most studies focus on the regulatory roles of phytochemicals on circRNAs. Abnormal expression of circRNAs has been identified in chronic diseases like cancer, heart failure, depression and atherosclerosis, and numerous studies have revealed the modulation of circRNAs by phytochemicals including berberine, celastrol, cinnamaldehyde, curcumin, et al. The expression of circRNAs, such as circSATB2 and circFOXM1, were modulated by phytochemicals, and these regulations further affected cell proliferation, apoptosis, migration, invasion, autophagy, chemosensitivity, radiosensitivity and other biological processes. Mechanismly, the circRNAs mainly functioned as miRNA sponge, subsequently affecting miRNA-mediated regulation of target genes and related cell signaling pathways. In this review, we summarized the impact of phytochemicals on circRNAs expression and biological function, and discussed the mechanisms underlying phytochemicals regulating circRNAs in cancer and other chronic diseases.
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http://dx.doi.org/10.1016/j.phrs.2021.105936DOI Listing
December 2021

Sigma-1 Receptor Activation Suppresses Microglia M1 Polarization via Regulating Endoplasmic Reticulum-Mitochondria Contact and Mitochondrial Functions in Stress-Induced Hypertension Rats.

Mol Neurobiol 2021 Dec 2;58(12):6625-6646. Epub 2021 Oct 2.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.

Exposure to stress plays a detrimental role in the pathogenesis of hypertension via neuroinflammation pathways. Microglial neuroinflammation in the rostral ventrolateral medulla (RVLM) exacerbates stress-induced hypertension (SIH) by increasing sympathetic hyperactivity. Mitochondria of microglia are the regulators of innate immune response. Sigma-1R (σ-1R) localizes to the mitochondria-associated membranes (MAMs) and regulates endoplasmic reticulum (ER) and mitochondria communication, in part through its chaperone activity. The present study aims to investigate the protective role of σ-1R on microglial-mediated neuroinflammation. Stress-induced hypertension (SIH) was induced in rats using electric foot shocks and intermittent noise. Arterial blood pressure (ABP), heart rate (HR), and renal sympathetic nerve activity (RSNA) were measured to evaluate the sympathetic nervous system (SNS) activities. SKF10047 (100 µM), an agonist of σ-1R, was administrated to rats, then σ-1R localization and MAM alterations were detected by immuno-electron microscopy. Mitochondrial calcium homeostasis was examined in primary microglia and/or BV-2 microglia cells. The effect of SKF10047 treatment on the mitochondrial respiratory function of cultured microglia was measured using a Seahorse Extracellular Flux Analyzer. Confocal microscopic images were performed to indicate mitochondrial dynamics. Stress reduces σ-1R's localization at the MAMs, leading to decreased ER-mitochondria contact and IP3R-GRP75-VDAC calcium transport complexes expression in the RVLM of rats. SKF10047 promotes the length and coverage of MAMs in the prorenin-treated microglia. Prorenin treatment increases mitoROS levels, and inhibits Ca signalling between the two organelles, therefore negatively affects ATP production in BV2 cells, and these effects are reversed by SKF10047 treatment. We found mitochondrial hyperfusion and microglial M1 polarization in prorenin-treated microglia. SKF10047 suppresses microglial M1 polarization and RVLM neuroinflammation, subsequently ameliorates sympathetic hyperactivity in stress-induced hypertensive rats. Sigma-1 receptor activation suppresses microglia M1 polarization and neuroinflammation via regulating endoplasmic reticulum-mitochondria contact and mitochondrial functions in stress-induced hypertension rats.
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http://dx.doi.org/10.1007/s12035-021-02488-6DOI Listing
December 2021

The Association of Systemic Interleukin 6 and Interleukin 10 Levels with Sarcopenia in Elderly Patients with Chronic Obstructive Pulmonary Disease.

Int J Gen Med 2021 18;14:5893-5902. Epub 2021 Sep 18.

Department of Orthopedics, The Sixth Hospital of Shanxi Medical University, Taiyuan, 030000, People's Republic of China.

Objective: Chronic obstructive pulmonary disease (COPD) is often associated with sarcopenia, and systemic inflammation is an underlying pathogenesis of COPD. In this study, the effects of interleukin (IL)- 6 and IL-10 levels on sarcopenia in patients with COPD were investigated.

Methods: Seventy-three patients (59 males and 14 females), aged 60-93 years old, who had stable COPD (not including patients with advanced lung decline) were enrolled in this study. The patients were classified according to four stages of lung function, based on the Global Initiative for Chronic Obstructive Lung Disease 2018, and their symptoms were evaluated using the COPD assessment test and mMRC dyspnea Scale. The skeletal muscle index (SMI) was evaluated using a dual-energy X-ray absorptiometry test, and walking pace and handgrip strength were measured to reflect muscle mass and function. The diagnosis of sarcopenia was made according to the Asian Working Group for Sarcopenia and China guide recommendations. The inflammation biomarkers IL-6 and IL-10 were measured by enzyme-linked immunosorbent assay. Albumin (Alb) was measured using the bromocresol green method.

Results: Twenty-eight patients (38.36%) were diagnosed with sarcopenia. IL-6 (r = -0.38, = 0.001) and IL-10 (r = -0.38, = 0.001) were negatively correlated with the SMI, while the IL-10/IL-6 ratio had no correlation with sarcopenia. Multiple regression analysis showed that age (OR = 1.11, = 0.02) and IL-6 (OR = 1.07, <0.05), had a strong correlation with sarcopenia, while IL-10 was not statistically significant in sarcopenia. However, body mass index (OR = 0.54, = 0.002) had an opposite effect on muscle reduction.

Conclusion: The increase of IL-6 might be a factor associated with sarcopenia. However, the relationship between IL-10 and sarcopenia is unclear.
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http://dx.doi.org/10.2147/IJGM.S321229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457863PMC
September 2021

Development of cationic peptide chimeric lysins based on phage lysin Lysqdvp001 and their antibacterial effects against Vibrio parahaemolyticus: A preliminary study.

Int J Food Microbiol 2021 Nov 8;358:109396. Epub 2021 Sep 8.

College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province 266003, PR China. Electronic address:

Cationic peptide chimeric lysins, Lysqdvp001-5aa, Lysqdvp001-10aa and Lysqdvp001-15aa, were designed based on lysin Lysqdvp001 from Vibrio parahaemolyticus (V. parahaemolyticus) phage qdvp001. These chimeric lysins showed equivalent peptidoglycan hydrolysis activities with Lysqdvp001 and could lyse the bacteria from the outside. The antibacterial activity as well as outer and inner membrane permeabilization of Lysqdvp001 and chimeric lysins against V. parahaemolyticus were Lysqdvp001-15aa>Lysqdvp001-10aa>Lysqdvp001-5aa>Lysqdvp001. Lysqdvp001-15aa exhibited an excellent antibacterial activity with minimum inhibition and bactericidal concentrations (MIC and MBC) of 0.2 and 0.4 mg/mL, respectively, and its antibacterial spectrum was much broader than phage qdvp001. Membrane hyperpolarization and membrane phospholipid exposure of V. parahaemolyticus were observed after Lysqdvp001-15aa treatments. Transmission electron microscope (TEM) showed Lysqdvp001-15aa destroyed structure integrity of V. parahaemolyticus. Besides, MIC and MBC of Lysqdvp001-15aa decreased V. parahaemolyticus counts in oyster by 3.20 and 4.03 logCFU/g. Lysqdvp001-15aa at MBC eradicated about 50% of V. parahaemolyticus biofilms and inhibited over 90% of the formation of the bacterial biofilms.
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http://dx.doi.org/10.1016/j.ijfoodmicro.2021.109396DOI Listing
November 2021

Causal Associations of Obesity With Chronic Kidney Disease and Arterial Stiffness: A Mendelian Randomization Study.

J Clin Endocrinol Metab 2022 Jan;107(2):e825-e835

Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Context: Observational studies have been associated obesity with chronic kidney disease (CKD) and arterial stiffness, but the causality remains unclear.

Objective: We aimed to investigate the causality of obesity with CKD and arterial stiffness using mendelian randomization (MR) analysis.

Methods: We genotyped 14 body mass index (BMI)-associated variants validated in East Asians in 11 384 Chinese adults. A genetic risk score based on the 14 variants and the 14 individual single-nucleotide variations (SNVs, formerly single-nucleotide polymorphisms [SNPs]) were respectively used as instrumental variables (IVs). CKD was defined as estimated glomerular filtration rate less than 60 mL/min/1.73 m2. Arterial stiffness was defined as brachial-ankle pulse wave velocity greater than 1550 cm/s.

Results: Using the genetic risk score as the IV, we demonstrated causal relations of each 1-SD increment in BMI with CKD (odds ratio [OR]: 2.36; 95% CI, 1.11-5.00) and arterial stiffness (OR: 1.71; 95% CI, 1.22-2.39). Using the 14 SNVs individually as IVs, each 1-SD increment in BMI was casually associated with CKD (OR: 2.58; 95% CI, 1.39-4.79) and arterial stiffness (OR: 1.87; 95% CI, 1.24-2.81) in the inverse-variance weighted analysis, and MR-Egger regression revealed no evidence of horizontal pleiotropy (both P for intercept ≥ .34). The causality between obesity and CKD was validated in 2-sample MR analysis among Europeans (681 275 of Genetic Investigation of ANthropometric Traits and 133 413 of CKD Genetics).

Conclusion: This study provided novel insights into the causality of obesity with CKD and arterial stiffness, highlighting the importance of weight management for primary prevention and control of subclinical vascular diseases.
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http://dx.doi.org/10.1210/clinem/dgab633DOI Listing
January 2022

Reducing the Allergenicity of Shrimp Tropomyosin and Allergy Desensitization Based on Glycation Modification.

J Agric Food Chem 2021 Dec 24;69(49):14742-14750. Epub 2021 Aug 24.

Laboratory of Food Safety, College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, People's Republic of China.

Shrimp is a major allergic food that could trigger severe food allergy, with the most significant and potent allergen of shrimp referred to as tropomyosin (TM). Glycation modification (Maillard reaction) could reportedly weaken the allergenicity of TM and generate hypoallergenic TM, while up to now, there is still a lack of investigations on the hypoallergenic glycated tropomyosin (GTM) as a candidate immunotherapy for desensitizing the shrimp TM-induced allergy. This study analyzed the effects of glycation modification on decreasing the allergenicity of TM and generated hypoallergenic GTM and how GTM absorbed to the Al(OH) function as a candidate immunotherapy for desensitizing allergy. As the results, in comparison to TM, the saccharides of smaller molecular sizes could lead to more advanced glycation end products in GTMs than saccharides of greater molecular sizes, and TM glycated by saccharides of different molecular sizes (glucose, maltose, maltotriose, maltopentaose, and maltoheptaose) exhibited lower allergenicity as a hypoallergen upon activating the allergic reactions of the mast cell and mouse model, while TM glycated by maltose had insignificant allergenicity changes upon activating the allergic reactions of the mast cell and mouse model. In addition, the hypoallergenic GTM + Al(OH) was efficient as a candidate immunotherapy; this work intended to offer preclinical data to promote GTM + Al(OH) as a candidate allergen-specific immunotherapy for desensitizing the allergy reactions for patients allergic to shrimp food.
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http://dx.doi.org/10.1021/acs.jafc.1c03953DOI Listing
December 2021

CO2 pneumoperitoneum pressure: an important factor influenced ovarian function after laparoscopy.

Ann Palliat Med 2021 08 20;10(8):9326-9327. Epub 2021 Jul 20.

Department of Obstetrics and Gynecology, The Maternal and Child Health Care Hospital of Guizhou Medical University, Guiyang, China.

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http://dx.doi.org/10.21037/apm-2021-05DOI Listing
August 2021
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