Publications by authors named "Hong Li"

5,277 Publications

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Randomized Control Study on Hemoperfusion Combined with Hemodialysis versus Standard Hemodialysis: Effects on Middle-Molecular-Weight Toxins and Uremic Pruritus.

Blood Purif 2022 Aug 11:1-11. Epub 2022 Aug 11.

Department of Nephrology, Shanghai Sixth People's Hospital, Shanghai, China.

Introduction: Classic hemodialysis schedules present inadequate middle-molecular-weight toxin clearance due to limitations of membrane-based separation processes. Accumulation of uremic retention solutes may result in specific symptoms (e.g., pruritus) and may affect clinical outcome and patient's quality of life. Hemoperfusion (HP) is a blood purification modality based on adsorption that can overcome such limitations, and thus, it may be interesting to test the efficacy of at least one session per week of HP combined with hemodialysis. This is a randomized, open-label trial, controlled, multicenter clinical study to investigate the effect of long-term HP combined with hemodialysis on middle-molecular-weight toxins and uremic pruritus in maintenance hemodialysis (MHD) patients.

Methods: 438 MHD patients from 37 HD centers in China with end-stage kidney disease (63.9% males, mean age 51 years) suffering from chronic intractable pruritus were enrolled in the study. Eligible patients were randomized into four groups: low-flux hemodialysis (LFHD), high-flux hemodialysis (HFHD), HP + LFHD, and HP + HFHD at a 1:1:1:1 ratio. Beta-2 microglobulin (β2M) and parathyroid hormone (PTH) were measured at baseline, 3-6, and 12 months. At the same time points, the pruritus score was evaluated. The primary outcome was the reduction of β2M and PTH, while the secondary outcome was the reduction of the pruritus score.

Results: In the two groups HP + LFHD and HP + HFHD, there was a significant decrease of β2M and PTH levels after 12 months compared to the control groups. No significant differences were noted between HP + LFHD and HP + HFHD. Pruritus score reduction was 63% in the HP + LFHD group and 51% in the HP + HFHD group, respectively.

Conclusion: The long-term HP + HD can reduce β2M and PTH levels and improve pruritus in MHD patients independently on the use of high- or low-flux dialyzers, showing that the results are linked to the effect of adsorption.
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http://dx.doi.org/10.1159/000525225DOI Listing
August 2022

Plasticity in astrocyte subpopulations regulates heroin relapse.

Sci Adv 2022 Aug 10;8(32):eabo7044. Epub 2022 Aug 10.

Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA.

Opioid use disorder (OUD) produces detrimental personal and societal consequences. Astrocytes are a major cell group in the brain that receives little attention in mediating OUD. We determined how astrocytes and the astroglial glutamate transporter, GLT-1, in the nucleus accumbens core adapt and contribute to heroin seeking in rats. Seeking heroin, but not sucrose, produced two transient forms of plasticity in different astroglial subpopulations. Increased morphological proximity to synapses occurred in one subpopulation and increased extrasynaptic GLT-1 expression in another. Augmented synapse proximity by astroglia occurred selectively at D2-dopamine receptor-expressing dendrites, while changes in GLT-1 were not neuron subtype specific. mRNA-targeted antisense inhibition of either morphological or GLT-1 plasticity promoted cue-induced heroin seeking. Thus, we show that heroin cues induce two distinct forms of transient plasticity in separate astroglial subpopulations that dampen heroin relapse.
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http://dx.doi.org/10.1126/sciadv.abo7044DOI Listing
August 2022

Purvalanol A induces apoptosis and reverses cisplatin resistance in ovarian cancer.

Anticancer Drugs 2022 Aug 9. Epub 2022 Aug 9.

Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan, Hubei Province 430060, P.R. China.

Cisplatin (DDP) resistance limits therapeutic efficacy in patients diagnosed with ovarian cancer. Purvalanol A (Pur) is a novel cyclin-dependent kinase (CDK) inhibitor that has been demonstrated to induce apoptosis in various cancer cells. The present study investigated the effect of the combination treatment of Pur and DDP, and the potential anticancer mechanisms in epithelial ovarian cancer (EOC) cells in vitro and in vivo. We found that Pur enhanced the anti-tumor efficacy of cisplatin in EOC cells. The combination of Pur and DDP had more significant effects on apoptosis induction in EOC cells compared with the individual-treatment groups and the control group. We further demonstrated that the combination of Pur and DDP may trigger apoptosis and autophagy in EOC cells by inducing reactive oxygen species (ROS). And the ROS/Akt/mammalian target of rapamycin signaling pathway as a potential mechanism for the initiation of autophagy induced by combination therapy. Similar results were observed in vivo. These results demonstrated that Pur sensitized the response of EOC cells to cisplatin in vitro and in vivo, reversing the resistance to cisplatin in ovarian cancer.
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http://dx.doi.org/10.1097/CAD.0000000000001339DOI Listing
August 2022

Frequently Expressed Glypican-3 As A Promising Novel Therapeutic Target for Osteosarcomas.

Cancer Sci 2022 Aug 9. Epub 2022 Aug 9.

South China University of Technology School of Medicine, Guangzhou, China.

Osteosarcoma (OS) is the most common bone malignancy without a reliable therapeutic target. Glypican-3 (GPC3) mutation and upregulation have been detected in multi-drug resistant OS, and anti-GPC3 immunotherapy can effectively suppress the growth of organoids. Further profiling of GPC3 mutations and expression patterns in OS is of clinical significance. To address these issues, fresh OS specimens were collected from 24 patients for cancer-targeted next-generation sequencing (NGS) and three-dimensional patient-derived organoid (PDO) culture. A tumor microarray was prepared using 37 archived OS specimens. Immunohistochemical (IHC) staining was performed on OS specimens and microarrays to profile GPC3 and CD133 expression as well as intratumoral distribution patterns. RT-PCR was conducted to semi-quantify GPC3 and CD133 expression levels in the OS tissues. Anti-GPC3 immunotherapy was performed on OS organoids with or without GPC3 expression and its efficacy was analyzed using multiple experimental approaches. No OS cases with GPC3 mutations were found, except for the positive control (OS-08). IHC staining revealed GPC3 expression in 73.77% (45/61) of OSs in weak (+; 29/45), moderate (++; 8/45), and strong (+++; 8/45) immunolabeling densities. The intratumoral distribution of GPC3-positive cells was variable in the focal (+; 10-30%; 8/45), partial (++; 31-70%; 22/45), and the most positive patterns (+++; > 71%; 15/45), which coincided with CD133 immunolabeling (P = 9.89×10 ). The anti-GPC3 antibody efficiently inhibits Wnt/β-catenin signaling and induces apoptosis in GPC3-positive PDOs and PDXs, as opposed to GPC3-negative PDOs and PDXs. The high frequency of GPC3 and CD133 co-expression and the effectiveness of anti-wildtype GPC3-ab therapy in GPC3-positive OS models suggest that GPC3 is a novel prognostic parameter and a promising therapeutic target for osteosarcoma.
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http://dx.doi.org/10.1111/cas.15521DOI Listing
August 2022

BRD7 restrains TNF-α-induced proliferation and migration of airway smooth muscle cells by inhibiting notch signaling.

Exp Lung Res 2022 Aug 9:1-14. Epub 2022 Aug 9.

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.

: Bromodomain-containing protein 7 (BRD7) is a key component of the switch/sucrose non-fermentable complex that participates in chromatin remodeling and transcriptional regulation. Although the emerging role of BRD7 in the pathophysiology of various diseases has been observed, its role in asthma remains unknown. Here, we assessed the function of BRD7 as a mediator of airway remodeling in asthma using an model. Airway smooth muscle cells (ASMCs) were challenged with tumor necrosis factor-α (TNF-α) to establish an airway remodeling model. Protein levels were examined using western blotting. Cell proliferation was measured using the cell counting kit-8 and 5-ethynyl-2'-deoxyuridine assays. Cell migration was assessed using a transwell migration assay. Exposure to TNF-α dramatically decreased BRD7 levels in ASMCs. BRD7 remarkably decreased TNF-α-induced proliferation and migration of ASMCs. In contrast, ASMCs with BRD7 deficiency were more sensitive to TNF-α-induced pro-proliferative and pro-migratory effects. Mechanistically, BRD7 could repress the expression of Notch1 and block the Notch pathway in TNF-α-challenged cells. Notably, reactivation of Notch signaling substantially reversed the BRD7 overexpression-mediated effects, whereas restraining Notch signaling abolished BRD7-depletion-mediated effects on TNF-α-challenged cells. BRD7 inhibits the proliferation and migration of ASMCs elicited by TNF-α by downregulating the Notch pathway. This study indicates that BRD7 may exert a suppressive effect on airway remodeling during asthma.
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http://dx.doi.org/10.1080/01902148.2022.2107730DOI Listing
August 2022

The Different Effects of Skeletal Muscle and Fat Mass on Height Increment in Children and Adolescents Aged 6-11 Years: A Cohort Study From China.

Front Endocrinol (Lausanne) 2022 22;13:915490. Epub 2022 Jul 22.

Department of Endocrinology and Metabolism, School of Medicine, Zhejiang University Affiliated Sir Run Shaw Hospital, Hangzhou, China.

Objective: This study aimed to investigate the contribution of body composition including skeletal muscle mass (SMM) and body fat mass (BFM) to longitudinal growth among children and adolescents aged 6-11 years old.

Methods: This cohort study was conducted from the annual health examination between 2019 and 2020. Annual height gain and weight gain and changes in SMM and BFM were calculated and compared between sexes, different nutritional status, and growth curve shifting mode. Spearman analyses and multiple linear regression analysis were performed to identify the impact of SMM, BFM, or body mass index (BMI) on height gain.

Results: Of the 584 subjects, the annual height gains of boys (4.76 cm in the 6-9-year group and 4.63 cm in the 10-11-year group) were significantly lower than those of girls (5.48 and 5.74 cm, respectively). Spearman analysis showed that SMM gain and height gain were positively and significantly correlated in each examination of all children ( = 0.535 for boys and 0.734 for girls, < 0.001). Conversely, BFM and height gains were negatively ( = -0.5240 for boys and -0.232 for girls, < 0.001) correlated. Multiple linear regression analysis identified SMM gain as an independent predictor (95% CI: 1.20,1.44) of height gain after adjusting for age, gender, BMI, BFM gain, and percentage of body fat (PBF).

Conclusion: SMM gains, rather than BFM gains, were associated with height gains in children and adolescents aged 6-11 years. Monitoring SMM changes in routine healthcare might motivate children and adolescents to achieve dietary and exercise recommendations, thereby growing taller without gaining excessive weight.
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http://dx.doi.org/10.3389/fendo.2022.915490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352863PMC
August 2022

Effect of Self-Determination Theory on Knowledge, Treatment Adherence, and Self-Management of Patients with Maintenance Hemodialysis.

Contrast Media Mol Imaging 2022 20;2022:1416404. Epub 2022 Jul 20.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Our aim of this study was to investigate the effect of self-determination theory on awareness of relevant knowledge, treatment compliance, and self-management level in maintenance hemodialysis (MHD) patients. A total of 90 patients who received MHD treatment in our hospital from April 2018 to June 2020 were selected and randomly divided into the intervention group and the routine group with 45 cases in each group. Both groups were given basic hemodialysis patient management measures, and the intervention group was given self-determination theory to manage patients on this basis. The awareness rate of hemodialysis patient-related knowledge, patient treatment compliance, patient self-management scores, and quality of life scores were compared between the two groups before intervention and 6 months after intervention. After intervention, the awareness rate of dialysis principle, reasonable diet, internal fistula protection, and complication prevention knowledge in the intervention group was higher ( < 0.05); before intervention, there was no significant difference in the scores of all dimensions of compliance between the two groups ( > 0.05); after intervention, the dietary compliance, fluid intake compliance, dialysis regimen compliance, and total score of compliance in the intervention group were higher than those in the conventional group ( < 0.05). After intervention, the total scores of problem solving, partnership, emotional processing, self-care activities, and self-management in the intervention group were higher than those in the conventional group, ( < 0.05). After intervention, the physical domain, psychological domain, social relationship domain, and total scores of quality of life in the intervention group were higher than those in the conventional group ( < 0.05). Self-determination theory management is effective in improving the awareness of hemodialysis-related knowledge, treatment compliance, self-management level, and quality of life in MHD patients.
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http://dx.doi.org/10.1155/2022/1416404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329035PMC
August 2022

High-Efficiency Near-Infrared Light Responsive Antibacterial System for Synergistic Ablation of Bacteria and Biofilm.

ACS Appl Mater Interfaces 2022 Aug 5. Epub 2022 Aug 5.

Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.

Bacterial infection is seriously threatening human health, and the design of high-efficiency and good biocompatibility antibacterial agents is an urgent problem to be solved. However, with the emergence of drug-resistant bacteria, the existing antibacterial agents have low killing efficiency, and the formation of biofilms has further weakened the therapeutic effect. Herein, we constructed an efficient antibacterial system mediated by near-infrared light for synergistic antibacterial and biofilm dissipation. Specifically, the ZnO/TiCT with heterojunction was synthesized by hydrothermal growth of ZnO on the surface of lamellar TiCT-MXene. The prepared ZnO/TiCT had better photothermal ability than ZnO and TiCT, respectively. The local thermal effect can not only destroy the integrity of the bacterial membrane but also promote the release of Zn ions and further improve the antibacterial performance. ZnO/TiCT achieved a 100% sterilization rate (better than either ZnO or TiCT) at 150 μg mL. The biofilm dissipation experiment further proved its excellent biofilm ablation effect. More importantly, the results of cell culture and animal experiments have demonstrated its good biological safety. In summary, this new type of nanomaterial shows strong local chemical photothermal sterilization ability and has great potential to replace traditional antibacterial agents.
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http://dx.doi.org/10.1021/acsami.2c08406DOI Listing
August 2022

Effects of Periodontal Treatment on Levels of Proinflammatory Cytokines in Patients with Chronic Periodontitis: A Meta-Analysis.

Authors:
Jiahui Ren Hong Li

Comput Math Methods Med 2022 26;2022:9349598. Epub 2022 Jul 26.

Dental Department, Xi'an Union Hospital, Xi'an City, Shaanxi Province 710199, China.

Background: During the progression of chronic periodontitis (CP), changes in the levels of inflammatory factors are detected in serum and gingival sulcus fluid (GCF). The aim of this meta-analysis was to systematically evaluate the effect of periodontal treatment on GCF and serum proinflammatory cytokines (IL-6, TNF-, and IL-8) in patients with CP.

Methods: Literature searches were performed through PubMed, Web of Science, Embase, China National Knowledge Infrastructure (CNKI), and Wanfang Database. Randomized controlled trials comparing cytokine levels in periodontal treatment (experimental group) and control group between 2015 and 2020 were included.

Results: There were a total of 13 studies included with 1220 patients. There were 630 cases in the experimental group and 590 cases in the control group. The meta-analysis showed that IL-6 levels in the GCF (SMD = -2.88, 95% CI (-3.68, -2.09), < 0.001) and serum (SMD = -1.27, 95% CI (-1.72, -0.81), < 0.001) were significantly lower in the experimental group compared with those before treatment. In addition, IL-8 levels in the GCF (SMD = -2.08, 95% CI (-3.40, -0.76), < 0.001) and serum (SMD = -1.73, 95% CI (-2.76, -0.70), < 0.001) were decreased after periodontal treatment, but more than that, a decrease was observed in TNF- levels of GCF (SMD = -3.98, 95% CI (-5.23, -2.73), < 0.001) and serum (SMD = -1.80, 95% CI (-3.16, -0.45), < 0.001) after treatment.

Conclusion: After periodontal therapy, the proinflammatory cytokines in the GCF and serum of patients with CP were significantly decreased compared with those before treatment, and the efficacy was remarkable.
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http://dx.doi.org/10.1155/2022/9349598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345718PMC
August 2022

Non-invasive brain stimulation associated mirror therapy for upper-limb rehabilitation after stroke: Systematic review and meta-analysis of randomized clinical trials.

Front Neurol 2022 19;13:918956. Epub 2022 Jul 19.

Department of Rehabilitation Medicine, Shijiazhuang People's Hospital, Shijiazhuang, China.

Background: Non-invasive brain stimulation (NIBS) techniques and mirror therapy (MT) are promising rehabilitation measures for stroke. While the combination of MT and NIBS has been employed for post-stroke upper limb motor functional rehabilitation, its effectiveness has not been examined.

Objective: This study aimed to evaluate the effectiveness of combined MT and NIBS in the recovery of upper limb motor function in stroke patients.

Methods: The search was carried out in PubMed, EMBASE, Cochrane Library, Web of Science, Science Direct, CNKI, WANFANG and VIP until December 2021. Randomized clinical trials (RCTs) comparing MT or NIBS alone with the combination of NIBS and MT in improving upper extremity motor recovery after stroke were selected. A meta-analysis was performed to calculate the mean differences (MD) or the standard mean differences (SMD) and 95% confidence intervals (CI) with random-effect models. Subgroup analyses were also conducted according to the types of control group, the types of NIBS, stimulation timing and phase poststroke.

Results: A total of 12 articles, including 17 studies with 628 patients, were reviewed in the meta-analysis. In comparison with MT or NIBS alone, the combined group significantly improved body structure and function (MD = 5.97; 95% CI: 5.01-6.93; < 0.05), activity levels (SMD = 0.82; 95% CI 0.61-1.02; < 0.05). For cortical excitability, the motor evoked potential cortical latency (SMD = -1.05; 95% CI:-1.57--0.52; < 0.05) and the central motor conduction time (SMD=-1.31 95% CI:-2.02--0.61; < 0.05) of the combined group were significantly shortened. A non-significant homogeneous summary effect size was found for MEP amplitude (SMD = 0.47; 95%CI = -0.29 to 1.23; = 0.23). Subgroup analysis showed that there is an interaction between the stimulation sequence and the combined treatment effect.

Conclusion: In this meta-analysis of randomized clinical trials, in comparison to the control groups, MT combined with NIBS promoted the recovery of upper extremity motor function after stroke, which was reflected in the analysis of body structure and function, activity levels, and cortical excitability.

Systematic Review Registration: https://www.crd.york.ac.uk/prospero/, identifier CRD42022304455.
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http://dx.doi.org/10.3389/fneur.2022.918956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345505PMC
July 2022

Whole cell response to receptor stimulation involves many deep and distributed subcellular biochemical processes.

J Biol Chem 2022 Aug 1:102325. Epub 2022 Aug 1.

Department of Pharmacological Sciences and Institute for Systems Biomedicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States. Electronic address:

Neurite outgrowth is an integrated whole-cell response triggered by the cannabinoid-1 receptor (CB1R). We sought to identify the many different biochemical pathways that contribute to this whole cell response. To understand underlying mechanisms, we identified subcellular processes composed of one or more biochemical pathways and their interactions required for this response. Differentially expressed genes and proteins were obtained from bulk transcriptomics and proteomic analysis of extracts from cells stimulated by a CB1R agonist. We then used these differentially expressed genes and proteins to build networks of SCPs and their interactions by combining the expression data with prior pathway knowledge. From these SCP networks, we identified additional genes that when ablated, experimentally validated the SCP involvement in neurite outgrowth. Our experiments and informatics modeling allowed us to identify diverse SCPs such as those involved in pyrimidine metabolism, lipid biosynthesis, and mRNA splicing and stability, along with more predictable SCPs such as membrane vesicle transport and microtubule dynamics. We find that SCPs required for neurite outgrowth are widely distributed among many biochemical pathways required for constitutive cellular functions, several of which are termed 'deep', since they are distal to signaling pathways and the key SCPs directly involved in extension of the neurite. In contrast, 'proximal' SCPs are involved in microtubule growth and membrane vesicle transport dynamics required for neurite outgrowth. From these bioinformatics and dynamical models based on experimental data, we conclude that receptor-mediated regulation of subcellular functions for neurite outgrowth is both distributed, i.e., involves many different biochemical pathways, and deep.
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http://dx.doi.org/10.1016/j.jbc.2022.102325DOI Listing
August 2022

Pattern and Predictive Factors of Metastasis in Lymph Nodes Posterior to the Right Recurrent Laryngeal Nerve in Papillary Thyroid Carcinoma.

Front Endocrinol (Lausanne) 2022 18;13:914946. Epub 2022 Jul 18.

Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Objective: The right cervical central lymph nodes include lymph nodes anterior to the right recurrent laryngeal nerve (LN-arRLN) and lymph nodes posterior to the right recurrent laryngeal nerve (LN-prRLN), and are separated by the right recurrent laryngeal nerve (RLN). LN-prRLN is a common site of nodal recurrence after the resection of papillary thyroid carcinoma (PTC). However, the complexity in anatomical structure brings difficulties in determining the surgical scope, so it is necessary to assess the pattern and predictive factors of right cervical central lymph nodes, especially LN-prRLN metastasis in papillary thyroid carcinoma.

Methods: A total of 562 diagnosed PTC patients who underwent right or total thyroidectomy were enrolled in this retrospective study. The clinicopathological features were collected, univariate and multivariate analyses were performed to determine predictive factors of the right central lymph node metastasis.

Results: In this study, the metastatic rates of the right CLN, the LN-arRLN and the LN-prRLN were 59.6% (335/562), 51.8% (291/562) and 30.4% (171/562), respectively. And 22.6% (127/562) of patients had both LN-arRLN and LN-prRLN metastasis. Among patients without LN-arRLN metastasis, the rate of LN-prRLN metastasis was 16.2% (44/271), accounting for 25.7% of the LN-prRLN metastasis group. Factors associated with an increased risk of LN-arRLN metastasis include male, age below 55 years, tumor size > 1cm, extrathyroidal extension (ETE), clinical lymph nodes metastasis(cN1), lateral lymph node metastasis, and left CLN metastasis. In addition, ETE, lateral lymph node metastasis, and LN-arRLN metastasis were independent factors of LN-prRLN metastasis. The predictive factors of LN-prRLN in cN0 PTC were further explored, revealing that tumor size ≥1.5cm, ETE, and LN-arRLN metastasis were independent predictors of LN-prRLN metastasis in cN0 PTC.

Conclusion: The LN-prRLN should not be ignored in surgery because of its high rate of metastasis. Our findings indicate that thorough dissection of central lymph nodes, especially LN-prRLN is crucial in clinical work.
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http://dx.doi.org/10.3389/fendo.2022.914946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339603PMC
August 2022

Study on strain sensing property of fiber Bragg grating based on surface strain measurement of mechanical structure.

Rev Sci Instrum 2022 Jul;93(7):075003

Institute of Disaster Prevention, Sanhe 065201, Hebei, China.

Strain monitoring is of great significance to identify the failure of key mechanical components and ensure the good operation of mechanical equipment. In terms of the common issue of the low sensitivity of fiber Bragg grating (FBG) strain sensor in strain measurement on the mechanical structure surface, this paper describes a flexible hinge strain sensor with the FBG as the sensitive element. A theoretical analysis of the sensitivity of the sensor is performed, a copper alloy material with a large elastic modulus is selected, the simulation analysis and optimization design for the structural parameters of the sensor are carried out by using ANSYS and Solidworks software, then the object of the sensor is developed according to the simulation results, and finally, the performance of the sensor is tested. The results show that the maximum deformation the sensor can measure is 0.5299 µε, the sensitivity of the sensor is ∼1.8870 pm/µε, which is approximately twice that of the bare FBG sensor, the linearity is greater than 99%, and the relative standard deviation of the repeatability is 0.9%. The results of the study provide a reference for developing the optical fiber strain sensor and further improving its sensitivity.
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http://dx.doi.org/10.1063/5.0093022DOI Listing
July 2022

Image Compression Using Stochastic-AFD Based Multi-signal Sparse Representation.

IEEE Trans Image Process 2022 Aug 3;PP. Epub 2022 Aug 3.

Adaptive Fourier decomposition (AFD) is a newly developed signal processing tool that can adaptively decompose any single signal using a Szegö kernel dictionary. To process multiple signals, a novel stochastic-AFD (SAFD) theory was recently proposed. The innovation of this study is twofold. First, a SAFD-based general multi-signal sparse representation learning algorithm is designed and implemented for the first time in the literature, which can be used in many signal and image processing areas. Second, a novel SAFD based image compression framework is proposed. The algorithm design and implementation of the SAFD theory and image compression methods are presented in detail. The proposed compression methods are compared with 13 other state-of-the-art compression methods, including JPEG, JPEG2000, BPG, and other popular deep learning-based methods. The experimental results show that our methods achieve the best balanced performance. The proposed methods are based on single image adaptive sparse representation learning, and they require no pre-training. In addition, the decompression quality or compression efficiency can be easily adjusted by a single parameter, that is, the decomposition level. Our method is supported by a solid mathematical foundation, which has the potential to become a new core technology in image compression.
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http://dx.doi.org/10.1109/TIP.2022.3194696DOI Listing
August 2022

Seasonal variation in structure and function of gut microbiota in .

Ecol Evol 2022 Aug 29;12(8):e9162. Epub 2022 Jul 29.

College of Biology and the Environment, Nanjing Forestry University Nanjing China.

Gut microbiota is associated with host health and its environmental adaption, influenced by seasonal variation. is one of the world's 100 worst invasive alien species. Here, we used high-throughput sequencing of the 16S rRNA gene to analyze the seasonal variation of gut microbiota of . The results suggested that the predominant gut microbial phyla of included Firmicutes and Proteobacteria, which helped digest plant food and accumulate energy. The gut microbiota of in summer group showed the highest diversity, whereas the winter group possessed the lowest, probably due to the shortage of food resources of in winter. Principal coordinate analysis analysis based on unweighted unifrac and weighted unifrac indicated that the composition of gut microbiota of significantly varied across seasons. Bacteroidetes tended to be enriched in summer by linear discriminant analysis effect size analysis. Actinobacteria and Cyanobacteria were extremely abundant in autumn, while Fusobacteria and enriched in winter. In conclusion, the structure of the gut microbiota of was significantly different among seasons, which was beneficial to the environment adaptation and the digestion and metabolism of food during different periods.
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http://dx.doi.org/10.1002/ece3.9162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336170PMC
August 2022

Automatic evaluation of endometrial receptivity in three-dimensional transvaginal ultrasound images based on 3D U-Net segmentation.

Quant Imaging Med Surg 2022 Aug;12(8):4095-4108

State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China.

Background: Endometrial thickness is an essential factor affecting female fertility. Clinically, ultrasound imaging is the first choice for the examination of uterine and endometrial-related diseases. However, the boundary of some endometrial is challenging to distinguish due to the effects of image resolution and noise. In addition, the irregular shape of the endometrium makes it more difficult for doctors to measure its thickness. Through the automatic segmentation and extraction of the endometrium, the maximum thickness of the endometrium can be measured automatically and accurately. This provides a quantitative index for doctors to use diagnostically.

Methods: In this study, 85 cases of three-dimensional transvaginal ultrasound (3D TVUS) images were collected retrospectively, including 75 cases of endometrial adhesion and 10 cases of non-adhesion. Firstly, the ultrasound images were filtered by block-matching and 3D filtering and speckle reducing anisotropic diffusion (SRAD). These two kinds of filtered images were combined with the original image to construct a three-channel image. Then, the augmented images were sent to 3D U-Net to realize endometrium segmentation. The performance of the segmentation models was evaluated using the Dice similarity coefficient (DSC), Jaccard, sensitivity, and 95th percentile Hausdorff distance (HD95). Finally, the medial axis transform was used to extract the endometrial centerline, based on which the endometrial thickness could be automatically measured.

Results: The endometrium segmentation method proposed in this paper achieved 90.83% in Dice, 83.35% in Jaccard, 90.85% in sensitivity, and 12.75 mm in HD95 in the testing set. Taking the doctor's manual measurement as the gold standard, 94.20% of the automatic endometrial thickness measurements based on the segmentation results were within the allowable error range of clinical diagnosis.

Conclusions: This paper presents an automatic endometrium segmentation and thickness measurement method for 3D TVUS images. The experimental results show that this method has high segmentation accuracy to recognize endometrial adhesion images. Furthermore, the thickness measurement based on the segmentation results has high reliability and repeatability, and the accuracy can meet clinical diagnosis needs.
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http://dx.doi.org/10.21037/qims-21-1155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338380PMC
August 2022

Antepartum sleep quality, mental status, and postpartum depressive symptoms: a mediation analysis.

BMC Psychiatry 2022 08 2;22(1):521. Epub 2022 Aug 2.

Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, 200011, China.

Background: Poor sleep quality and maternal mood disturbances are common during pregnancy and may play pivotal roles in the development of postpartum depression. We aim to examine the trajectories of sleep quality and mental health in women from early pregnancy to delivery and explore the mediating effects of sleep quality and mental status on the link between antepartum depressive symptoms and postpartum depressive symptoms.

Methods: In an ongoing prospective birth cohort, 1301 women completed questionnaires in the first, second and third trimesters and at 6 weeks postpartum. In each trimester, sleep quality was measured utilizing the Pittsburgh Sleep Quality Index (PSQI), and mental health was assessed with the Center for Epidemiologic Studies Depression Scale (CES-D), the Self-Rating Anxiety Scale (SAS) and the Perceived Stress Scale (PSS). Postpartum depressive symptoms were evaluated by the Edinburgh Postnatal Depression Scale (EPDS). The bootstrap method was used to test the mediation effect.

Results: The PSQI, CES-D, and SAS scores presented U-shaped curves across the antenatal period while the PSS score followed a descending trend. Antenatal sleep quality, depressive symptoms, anxiety symptoms and perceived stress all predicted depressive symptoms at 6 weeks postpartum. The influence of antepartum depressive symptoms on postpartum depressive symptoms was mediated by antepartum sleep quality and anxiety symptoms, which accounted for 32.14%, 39.25% and 31.25% in the first, second and third trimesters (P = 0.002, P = 0.001, P = 0.001, respectively).

Conclusions: Poor sleep quality and anxiety symptoms in pregnancy mediated the relationship between antepartum depressive symptoms and postpartum depressive symptoms. Interventions aimed at detecting and managing sleep quality and elevated anxiety among depressed women in pregnancy warrant further investigation as preventative strategies for postpartum depression.
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http://dx.doi.org/10.1186/s12888-022-04164-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344627PMC
August 2022

A Role for Basigin in Toxoplasma gondii Infection.

Infect Immun 2022 Aug 1:e0020522. Epub 2022 Aug 1.

Department of Medicine, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.

The role of specific host cell surface receptors during Toxoplasma gondii invasion of host cells is poorly defined. Here, we interrogated the role of the well-known malarial invasion receptor, basigin, in T. gondii infection of astrocytes. We found that primary astrocytes express two members of the BASIGIN (BSG) immunoglobulin family, basigin and embigin, but did not express neuroplastin. Antibody blockade of either basigin or embigin caused a significant reduction of parasite infectivity in astrocytes. The specific role of basigin during T. gondii invasion was further examined using a mouse astrocytic cell line (C8-D30), which exclusively expresses basigin. CRISPR-mediated deletion of basigin in C8-D30 cells resulted in decreased T. gondii infectivity. T. gondii replication and invasion efficiency were not altered by basigin deficiency, but parasite attachment to astrocytes was markedly reduced. We also conducted a proteomic screen to identify T. gondii proteins that interact with basigin. -encoded cyclophilins, the protein 14-3-3, and protein disulfide isomerase (TgPDI) were among the putative basigin-ligands identified. Recombinant TgPDI produced in E. coli bound to basigin and pretreatment of tachyzoites with a PDI inhibitor decreased parasite attachment to host cells. Finally, mutagenesis of the active site cysteines of TgPDI abolished enzyme binding to basigin. Thus, basigin and its related immunoglobulin family members may represent host receptors that mediate attachment of T. gondii to diverse cell types.
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http://dx.doi.org/10.1128/iai.00205-22DOI Listing
August 2022

Application of General Anesthesia Combined with Saphenous Nerve-Tibial Nerve Block in Total Knee Arthroplasty.

Evid Based Complement Alternat Med 2022 19;2022:7010492. Epub 2022 Jul 19.

Anesthesiology Operating Room, Dongying People's Hospital, Dongying, Shandong, China.

Background: The study aimed to evaluate the safety and efficiency of the saphenous nerve plus selective tibial nerve block combined with general anesthesia in total knee replacement surgery (TKRS).

Methods: Sixty-four patients who underwent unilateral TKRS between October 2019 and June 2020 were selected as study subjects. All patients were divided into the control and observation groups using the random number table method, with 32 patients in each group. Conventional general anesthesia was performed preoperatively in both groups. The control group was given an ultrasound-guided saphenous nerve block before anesthesia induction, and the observation group was given a selective tibial nerve block on the basis of the control group. The dosage of general anesthetic drugs, recovery time from general anesthesia, hemodynamic index, inflammatory response, postoperative analgesic effect, and adverse reaction rate were compared between the two groups.

Results: Compared with the control group, the total amount of propofol and remifentanil used in the observation group was significantly less ( < 0.05). Compared with the control group, patients in the observation group experienced remarkably shorter time to recovery from respiration, time to extubation, and time in the PACU ( < 0.05). Compared with the control group, the observation group showed a significantly reduced SBP and MAP at T2, T3, and T4, respectively, and also showed a prominently lower HR at T3 and T4 ( < 0.05). Markedly lower CRP and IL-6 levels at 6 h and 24 h after surgery were found in the observation group compared to the control group ( < 0.05). Compared with the control group, patients receiving nerve block intervention got significantly lower VAS scores at 6 h, 24 h, and 48 h postoperatively ( < 0.05). However, there was no statistically significant difference in the incidence of adverse reactions between the two groups of patients ( > 0.05).

Conclusion: The application of the saphenous nerve plus selective tibial nerve block combined with general anesthesia in TKRS yields a promising analgesic effect, stable hemodynamics, low levels of postoperative inflammatory responses, and high safety.
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http://dx.doi.org/10.1155/2022/7010492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325614PMC
July 2022

Flexible Recyclable Cellulose Paper Templated Cu-Doped Polydopamine Membranes with Dual Enzyme-Like Activity.

Small 2022 Jul 30:e2202405. Epub 2022 Jul 30.

Beijing National Laboratory for Molecular Sciences, CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

The development of high-efficiency enzyme mimics is of great significance in the field of biocatalysis. However, it remains challenging to design novel enzyme mimics with multiple enzyme-like activities, excellent stability, and good reusability. Herein, a facile molecular assembly strategy to construct dialdehyde cellulose (DAC) templated Cu-doped polydopamine ([email protected]/Cu) membrane with dual enzyme-like activities is presented. The Schiff base bonds formed between polydopamine (PDA) and DAC can not only accelerate the adhesion of PDA thin layer but also contribute to Cu-loading and high stability of [email protected]/Cu membrane. Importantly, the assembled [email protected]/Cu membrane exhibits a remarkable catalytic activity that is superior to the natural laccase along with high stability and excellent reusability. Moreover, the [email protected]/Cu membrane also demonstrates peroxidase-like activity, and it is successfully applied in the sensitive detection of ascorbic acid (AA). This work will provide a new paradigm methodology for rational design and practical applications of enzyme mimics based on bioinspired molecular assemblies.
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http://dx.doi.org/10.1002/smll.202202405DOI Listing
July 2022

Vertical distribution of antibiotics and antibiotic resistance genes in a representative municipal solid waste landfill, China.

Ecotoxicol Environ Saf 2022 Jul 25;242:113919. Epub 2022 Jul 25.

College of Civil Engineering, Zhejiang University of Technology, Hangzhou 310023, China; Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province, Zhejiang University of Science and Technology, Hangzhou 310023, China. Electronic address:

The vertical distribution of sulfonamides (SAs), tetracyclines (TCs), macrolides (MLs), and their related antibiotic resistance genes (ARGs) were comprehensively investigated and characterized in a representative municipal solid waste (MSW) landfill in China. The total concentrations of target antibiotics in the MSW landfill were SAs > TCs > MLs. The abundances of mexF (10.78 ± 0.65 logcopies/g) and sul genes (9.15 ± 0.54 logcopies/g) were relatively high, while the tet genes (7.19 ± 0.77 logcopies/g) were the lowest. Both the abundance of antibiotics and genes fluctuated with landfill depth, and the ARGs of the same antibiotics were consistent with depth change. Intl1 and sul genes (sul1, sul2) were tightly connected, and a close relationship also existed between tet genes (tetM, tetQ) and MLs resistance genes (ermB, mefA). High-throughput sequencing showed the dominant genera were Sporosarcina (38%) and Thiobacillus (17%) at sampling points A and C, while the microbial community varied with depth increase at point B were Brevundimonas (20%), Sporosarcina (20%), Pseudomonas (24%), Lysobacter (28%), and Thioalkalimicrobium (14%), respectively. Network analysis further visualized the relationship among antibiotics, genes, and microbial communities and the results indicated the non-random connection among them and the possible host of the target gene. Even at 12.0 m below the landfill surface, the pollution of antibiotics resistance was still serious, which posed difficulties for subsequent landfill remediation and pollution control.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113919DOI Listing
July 2022

The Progress of Research into Flexible Sensors in the Field of Smart Wearables.

Sensors (Basel) 2022 Jul 6;22(14). Epub 2022 Jul 6.

College of Textile, Zhongyuan University of Technology, Zhengzhou 450007, China.

In modern society, technology associated with smart sensors made from flexible materials is rapidly evolving. As a core component in the field of wearable smart devices (or 'smart wearables'), flexible sensors have the advantages of excellent flexibility, ductility, free folding properties, and more. When choosing materials for the development of sensors, reduced weight, elasticity, and wearer's convenience are considered as advantages, and are suitable for electronic skin, monitoring of health-related issues, biomedicine, human-computer interactions, and other fields of biotechnology. The idea behind wearable sensory devices is to enable their easy integration into everyday life. This review discusses the concepts of sensory mechanism, detected object, and contact form of flexible sensors, and expounds the preparation materials and their applicability. This is with the purpose of providing a reference for the further development of flexible sensors suitable for wearable devices.
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http://dx.doi.org/10.3390/s22145089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319532PMC
July 2022

Tissue engineered bone via templated hBMSCs mineralization and its application for bone repairing.

Biomater Adv 2022 Aug 30;139:212937. Epub 2022 May 30.

College of Chemistry and Materials Science, Jinan University, No. 601, West Huangpu Avenue, Guangzhou, Guangdong 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, PR China. Electronic address:

To develop bone implants, a novel tissue-engineered bone was constructed via templated human bone mesenchymal stem cells (hBMSCs) mineralization. Firstly, an osteoid-like template (Os-template) with aligned collagen fibers was prepared and followed by seeding hBMSCs to mimic the process of bone formation. After being cultured over weeks, the cells produced collagen fibers in an orderly aligned osteomorphic fashion. Further, a novel tissue-engineered bone with mineralized collagen fiber (mOs-ECM) was subsequently achieved after cell mineralization, showing a high degree of osteomimicry in terms of both composition and structure. When applied to the rat cranial bone defect model, the mOs-ECM significantly promoted the new bone formation and fused with the host bone. The study indicated that microscopic cell mineralization could be guided by artificially designed templates and successfully fabricated a macroscopic implant with a pronounced effect on bone repairing.
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http://dx.doi.org/10.1016/j.bioadv.2022.212937DOI Listing
August 2022

Examining the independent and interactive association of physical activity and sedentary behaviour with frailty in Chinese community-dwelling older adults.

BMC Public Health 2022 07 26;22(1):1414. Epub 2022 Jul 26.

The Shengli Clinical Medical College of Fujian Medical University, 134 Dongjie Road, Fuzhou, Fujian, 350100, People's Republic of China.

Background: While physical inactivity or prolonged sitting has been linked to an increased risk of frailty, the interaction between sitting time (ST), physical activity (PA) and frailty is not well understood. The aim of this study was to examine the dose-response relationship between PA, ST and frailty and further to evaluate the interaction effect of PA and ST on frailty in the context of regular COVID-19 epidemic prevention and control in China.

Methods: A cross-sectional analysis was performed on 1458 participants (age ≥ 60) enrolled from a prospective cohort study of frailty in elderly people of Fujian Province. PA and ST levels were assessed using the International Physical Activity Questionnaire. A 40-item frailty index (FI) quantified frailty. Multivariable logistic regression and linear regression models were applied to examine the dose-response relationship between PA or ST and frailty level. Interaction plots were used to visualise the interaction effects of PA and ST on frailty.

Results: Compared with light PA, the odds ratios (ORs) for frailty were significantly lower for moderate PA (OR, 0.609 [95% CI, 0.419, 0.885], P < .001) and vigorous PA (OR, 0.399 [95% CI, 0.236,0.673], P < .001). Comparing subjects with ST <  4 h/day, those with ST ≥ 8 h/day were significantly more likely to be diagnosed with frailty (OR, 3.140 [95% CI, 1.932, 5.106], P < .001), 6-8 h/day (OR, 1.289 [95% CI, 0.835, 1.989], P >0.05), and 4-6 h/day (OR, 1.400 [95% CI, 0.972, 2.018], P >0.05). Each one unit increase in metabolic equivalents (h/day) of PA was related to an average 0.928 (0.887, 0.971) decrease in prevalence of frailty, while each one unit increase in sitting time (h/day) was related to average 1.114 (1.046,1.185) increase in prevalence of frailty. Negative interactive effects of PA and ST on frailty were observed (P < 0.001).

Conclusion: There are nonlinear and linear dose-response relationships between PA, SB and frailty respectively. In addition, excess ST may counteract the beneficial effects of PA on frailty. Interventions that focus on reducing excess ST may be effective strategies to reduce the risk of frailty and should be taken seriously by public health authorities, especially in the context of regular epidemic prevention and control in China.
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http://dx.doi.org/10.1186/s12889-022-13842-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313602PMC
July 2022

Brain structure and synaptic protein expression alterations after antidepressant treatment in a Wistar-Kyoto rat model of depression.

J Affect Disord 2022 Oct 22;314:293-302. Epub 2022 Jul 22.

Department of Psychiatry, First Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, China; Shanxi Provincial Key Laboratory of Brain Science and Neuropsychiatric Diseases, First Hospital of Shanxi Medical University, Taiyuan, China; Department of Mental Health, Shanxi Medical University, Taiyuan, China.; National Key Disciplines, Key Laboratory for Cellular Physiology of Ministry of Education, Department of Neurobiology, Shanxi Medical University, Taiyuan, China.. Electronic address:

Background: Structural MRI has demonstrated brain alterations in depression pathology and antidepressants treatment. While synaptic plasticity has been previously proposed as the potential underlying mechanism of MRI findings at a cellular and molecular scale, there is still insufficient evidence to link the MRI findings and synaptic plasticity mechanisms in depression pathology.

Methods: In this study, a Wistar-Kyoto (WKY) depression rat model was treated with antidepressants (citalopram or Jie-Yu Pills) and tested in a series of behavioral tests and a 7.0 MRI scanner. We then measured dendritic spine density within altered brain regions. We also examined expression of synaptic marker proteins (PSD-95 and SYP).

Results: WKY rats exhibited depression-like behaviors in the sucrose preference test (SPT) and forced swim test (FST), and anxiety-like behaviors in the open field test (OFT). Both antidepressants reversed behavioral changes in SPT and OFT but not in FST. We found a correlation between SPT performance and brain volumes as detected by MRI. All structural changes were consistent with alterations of the corpus callosum (white matter), dendritic spine density, as well as PSD95 and SYP expression at different levels. Two antidepressants similarly reversed these macro- and micro-changes.

Limitations: The single dose of antidepressants was the major limitation of this study. Further studies should focus on the white matter microstructure changes and myelin-related protein alterations, in addition to comparing the effects of ketamine.

Conclusion: Translational evidence links structural MRI changes and synaptic plasticity alterations, which promote our understanding of SPT mechanisms and antidepressant response in WKY rats.
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http://dx.doi.org/10.1016/j.jad.2022.07.037DOI Listing
October 2022

Harm or protection? Two-sided consequences of females' susceptible responses to multiple threats.

Behav Brain Sci 2022 07 25;45:e141. Epub 2022 Jul 25.

Institute for Brain and Psychological Sciences, Sichuan Normal University, Chengdu, Sichuan, Chinahttps://www.researchgate.net/profile/Hong-Li-67.

The target article presented a plausible argument that females' susceptibility to threats might be self-protection for , but some evidence requires scrutiny. We need to consider (1) the biases of narrative reviews, (2) subjective life quality, and (3) the shadow side of extreme reactions to threats before concluding that females' threat-based response is a self-protection mechanism that promotes survival.
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http://dx.doi.org/10.1017/S0140525X22000607DOI Listing
July 2022

Effects of an art-based intervention in older adults with mild cognitive impairment: a randomised controlled trial.

Age Ageing 2022 Jul;51(7)

Research Center for Nursing Theory and Practice, Fujian Provincial Hospital, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, China.

Background: Art-based interventions may delay cognitive decline and improve health-related outcomes in older adults with mild cognitive impairment (MCI).

Objective: To examine the effects of the Creative Expressive Arts-based Storytelling (CrEAS) program compared to active and waitlist controls on neurocognitive and other health-related outcomes in older people with MCI.

Design: Three-arm parallel-group, randomised controlled design.

Participants: One-hundred and thirty-five adults with MCI (mean age: 70.93 ± 6.91 years).

Methods: Participants were randomly assigned to intervention (CrEAS, n = 45), active control (n = 45) or waitlist control (n = 45) groups. Interventions were applied once per week for 24 weeks. The primary outcome was global cognitive function; secondary outcomes were specific cognition domains (memory, executive function, language and attention) and other health-related outcomes (anxiety, depression and quality of life [QoL]). All variables were measured at baseline (T0), 24-week follow-up (T1) and 48-week follow-up (T2).

Results: Participants in the CrEAS group showed significantly higher global cognitive function (adjusted mean difference [MD] = -0.905, 95% confidence interval [CI] -1.748 to -0.062; P = 0.038) and QoL (adjusted MD = -4.150, 95% CI -6.447 to -1.853; P = 0.001) and lower depression symptoms (adjusted MD = 2.902, 95% CI 0.699-5.104; P = 0.011) post-intervention at the 24-week follow-up compared with the active control group. At 48-week follow-up, only the Auditory Verbal Learning Test Immediate recall score was significantly improved compared with the active control group (adjusted MD = -2.941, 95% CI -5.262 to -0.620; P = 0.014).

Conclusions: Older adults with MCI who participated in the CrEAS program improved their neuropsychological outcomes and QoL and reduced their rate of cognitive deterioration.
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http://dx.doi.org/10.1093/ageing/afac144DOI Listing
July 2022

Development and evaluation of an Ultra-Wide Bandwidth based electronic hand hygiene monitoring system.

Am J Infect Control 2022 Jul 19. Epub 2022 Jul 19.

Department of Hospital-Acquired Infection Control, Beijing Jishuitan Hospital, The Forth Medical College of Peking University, Beijing, China; Department of Burns and Plastic Surgery, Beijing Jishuitan Hospital, The Forth Medical College of Peking University, Beijing, China. Electronic address:

Background: A large variety of electronic hand hygiene monitoring systems (EHHMS) are being developed and applied in health care settings. Monitoring hand hygiene (HH) opportunity at bed-level has been the key technical challenge. Accuracy evaluation needs more attention as the prerequisite upon widespread acceptance and adoption.

Methods: For the first time, we explored, debugged and upgraded an EHHMS based on ultra-wide bandwidth (UWB) which can obtain HH opportunities at bed-level. The real-time positioning and electronic fence of UWB technology was applied for EHHMS. The accuracy of EHHMS was compared with the simultaneous manual direct observations in real-world clinical setting. Sensitivity and specificity were calculated for EHHMS capturing HH action and opportunity.

Results: Two generations of EHHMS were constructed. For the first generation, the system properly recorded 84.00% and 78% of the pre-identified HH actions and opportunities performed by experimenters. For the second generation, Sensitivity and specificity of the system capturing HH action were 89.00% (84.83-92.36) and 100.00% (98.26-100.00), respectively. For capturing HH opportunity, the system showed the sensitivity and specificity of 86.52% (82.52-89.89) and 88.10% (84.14-91.36)), respectively.

Conclusion: The EHHMS based on UWB could accurately identify HH action and opportunity with equivalent accuracy compared with simultaneous direct observation.
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http://dx.doi.org/10.1016/j.ajic.2022.07.008DOI Listing
July 2022

A novel treatment workflow of idiopathic hypereosinophilic syndrome: a single-center retrospective cohort study.

Chin Med J (Engl) 2022 Jul 25. Epub 2022 Jul 25.

Department of Allergy, Peking Union Medical College Hospital (PUMCH), Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS and PUMC), Beijing 100730, China.

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http://dx.doi.org/10.1097/CM9.0000000000002086DOI Listing
July 2022

Lithocholic acid inhibits dendritic cell activation by reducing intracellular glutathione via TGR5 signaling.

Int J Biol Sci 2022 11;18(11):4545-4559. Epub 2022 Jul 11.

The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, and Chongqing Branch of National Clinical Research Center for Ocular Diseases, Chongqing, P. R. China.

Dendritic cells (DCs) are the major antigen-presenting cells and play an important role in autoimmune uveitis. Emerging evidence suggests that bile acids (BAs) regulate DCs maturation. However, the underlying mechanisms by which BAs regulate the function of DCs still need to be clarified. Here, we demonstrate that lithocholic acid (LCA) inhibits the production of pro-inflammatory cytokines and the expression of surface molecules in bone marrow-derived dendritic cells (BMDCs). LCA attenuates the severity of EAU by modulating the maturation of splenic CD11CMHCII DCs. Notably, Takeda G-protein coupled receptor 5 (TGR5) deficiency partially reverses the inhibitory effect of LCA on DCs and . TGR5 activation also downregulates the NF-κB and MAPK pathways by inhibiting glutathione production and inducing oxidative stress in DCs, which leads to apoptosis and autophagy in DCs. In addition, LCA or INT-777 treatment increases the TGR5 expression in monocyte-derived dendritic cells (MD-DCs) of patients with active BD, whereas both LCA and TGR5 agonists inhibit the activation of MD-DCs. These results suggest that LCA and TGR5 agonists might be potential therapeutic drugs for the treatment of autoimmune uveitis.
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http://dx.doi.org/10.7150/ijbs.71287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295063PMC
July 2022
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