Publications by authors named "Hong Chen"

4,478 Publications

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How does government regulation promote green product diffusion in complex network? An evolutionary analysis considering supply side and demand side.

Authors:
Menghua Yang Hong Chen Ruyin Long Qingqing Sun Jiahui Yang

J Environ Manage 2022 Sep 4;318:115642. Epub 2022 Jul 4.

School of Economics and Management, China University of Mining and Technology, China.

China has launched a series of regulation policies that promote the diffusion of green products to drive the green development of resources and environment. This study proposes an evolutionary game model of green product diffusion by providing a joint "supply side - demand side" regulatory framework. It simulates the effects of government regulation on green product diffusion in complex network, the related numerical simulation analysis is carried out through a case of electric vehicles diffusion. The study confirms that (1) On the supply side, green subsidies, environmental taxes, and carbon trading market can successfully increase green product diffusion to 0.84, 0.7, and 0.65. On the demand side, green consumption vouchers, as well as publicity and education can increase green product diffusion to 0.7 and 0.67. (2) Among the order-based regulatory instruments, high environmental taxes and poor participation in carbon trading market can inhibit the spread of green products, while low green consumption vouchers fail to stimulate the purchase of green products. It is crucial to enhance emotion-based regulatory instruments like publicity and education. (3) Neither order-based nor emotion-based regulation can achieve complete diffusion of green products. This study provides new insights of green product diffusion under government regulation and its implementation effects.
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http://dx.doi.org/10.1016/j.jenvman.2022.115642DOI Listing
September 2022

Data Resource Profile: A Protocol of China National Diabetic Chronic Complications Study.

Authors:
Xu Hong Hou Li Min Wang Si Yu Chen Ye Bei Liang Mei Zhang Zheng Jing Huang Hong Li Chen Jing Zhu Wu Jing Wu Wei Ping Jia

Biomed Environ Sci 2022 Jul;35(7):633-640

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai 200233, China.

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http://dx.doi.org/10.3967/bes2022.078DOI Listing
July 2022

Insights into rapidly recovering the autotrophic nitrogen removal performance of single-stage partial nitritation-anammox systems: Reconstructing granular sludge and its functional microbes synergy.

Authors:
Enzhe Yang Jing Chen Ziyi Jiang Zhengyu Deng Zhi Tu Hong Wang Sha Wu Zhe Kong Eli Hendrik Sanjaya Hong Chen

Bioresour Technol 2022 Aug 6;361:127750. Epub 2022 Aug 6.

School of Hydraulic and Environmental Engineering, Changsha University of Science & Technology, Changsha 410114, China; Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, Changsha 410114, China. Electronic address:

Partial nitritation-anammox (PNA) deteriorates easily and is difficult to recover. After an airlift inner-circulation partition bioreactor was impacted by low NH-N wastewater containing organic matter, Nitrospira and Denitratisoma propagated rapidly, granular sludge disintegrated, and the total nitrogen removal efficiency (TNRE) decreased from 68.27 % to 5.97 %. This study used a unique strategy to recover deteriorated single-stage PNA systems and explored the mechanism of rapid performance recovery. The TNRE of the system recovered up to 61.77 % in 43 days. The high nitrogen loading rate and hydraulic shear force from the airlift caused the sludge in the reactor to granulate again. The microbial community structure recovered, with a decrease in the abundance of Nitrospira (0.05 %) and enrichment of Candidatus Brocadia (8.82 %). A favorable synergy among functional microbes in the reactor was thus re-established, promoting the rapid recovery of the nitrogen removal performance. This study provides a feasible recovery strategy for PNA processes.
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http://dx.doi.org/10.1016/j.biortech.2022.127750DOI Listing
August 2022

Poly(,-dimethyl)acrylamide-based ion-conductive gel with transparency, self-adhesion and rapid self-healing properties for human motion detection.

Authors:
Ling Wang Shengjie Liu Jingjing Cheng Yao Peng Fangfei Meng Zhaoqiang Wu Hong Chen

Soft Matter 2022 Aug 9. Epub 2022 Aug 9.

Key Laboratory of Polymeric Materials Design and Synthesis for Biomedical Function, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.

Flexible strain sensors have been extensively studied for their potential value in monitoring human activity and health. However, it is still challenging to develop multifunctional flexible strain sensors with simultaneously high transparency, strong self-adhesion, fast self-healing and excellent tensile properties. In this study, we used ,-dimethylacrylamide (DMA) in the imidazolium-based ionic liquid 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl] imide ([BMIM][TfN]) for "one-step" UV irradiation. A poly(,-dimethyl)acrylamide (PDMA) ion-conductive gel was prepared by site polymerization. Based on the good compatibility between PDMA and ionic liquid, the prepared ion-conductive gel has good transparency (∼90%), excellent stretchability (1080%), strong self-adhesion (67.57 kPa), fast self-healing (2 s at room temperature) and great antibacterial activity (∼99% bacterial killing efficiency). Moreover, the strain sensor based on the PDMA ion-conductive gel has good electromechanical performance and can detect different human motions. Based on the simple and easy-to-operate preparation method and the endowed multifunctionality of the PDMA ion-conductive gel, it has broad application prospects in the field of flexible electronic devices.
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http://dx.doi.org/10.1039/d2sm00786jDOI Listing
August 2022

GSDMD-dependent neutrophil extracellular traps promote macrophage-to-myofibroblast transition and renal fibrosis in obstructive nephropathy.

Authors:
Yujia Wang Yinshuang Li Zhimin Chen Ying Yuan Qinglin Su Keng Ye Caiming Chen Guoping Li Yankun Song Hong Chen Yanfang Xu

Cell Death Dis 2022 Aug 8;13(8):693. Epub 2022 Aug 8.

Department of Nephrology, Blood Purification Research Center, the First Affiliated Hospital, Fujian Medical University, Fuzhou, China.

Renal fibrosis is a common consequence of various progressive nephropathies, including obstructive nephropathy, and ultimately leads to kidney failure. Infiltration of inflammatory cells is a prominent feature of renal injury after draining blockages from the kidney, and correlates closely with the development of renal fibrosis. However, the underlying molecular mechanism behind the promotion of renal fibrosis by inflammatory cells remains unclear. Herein, we showed that unilateral ureteral obstruction (UUO) induced Gasdermin D (GSDMD) activation in neutrophils, abundant neutrophil extracellular traps (NETs) formation and macrophage-to-myofibroblast transition (MMT) characterized by α-smooth muscle actin (α-SMA) expression in macrophages. Gsdmd deletion significantly reduced infiltration of inflammatory cells in the kidneys and inhibited NETs formation, MMT and thereby renal fibrosis. Chimera studies confirmed that Gsdmd deletion in bone marrow-derived cells, instead of renal parenchymal cells, provided protection against renal fibrosis. Further, specific deletion of Gsdmd in neutrophils instead of macrophages protected the kidney from undergoing fibrosis after UUO. Single-cell RNA sequencing identified robust crosstalk between neutrophils and macrophages. In vitro, GSDMD-dependent NETs triggered p65 translocation to the nucleus, which boosted the production of inflammatory cytokines and α-SMA expression in macrophages by activating TGF-β1/Smad pathway. In addition, we demonstrated that caspase-11, that could cleave GSDMD, was required for NETs formation and renal fibrosis after UUO. Collectively, our findings demonstrate that caspase-11/GSDMD-dependent NETs promote renal fibrosis by facilitating inflammation and MMT, therefore highlighting the role and mechanisms of NETs in renal fibrosis.
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http://dx.doi.org/10.1038/s41419-022-05138-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360039PMC
August 2022

Comparison of the cumulative live birth rates after 1 in vitro fertilization cycle in women using gonadotropin-releasing hormone antagonist protocol vs. progestin-primed ovarian stimulation: a propensity score-matched study.

Authors:
Hong Chen Xiao Ming Teng Zi Li Sun Di Yao Zheng Wang Zhi Qin Chen

Fertil Steril 2022 Aug 5. Epub 2022 Aug 5.

Center of Assisted Reproduction, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China. Electronic address:

Objective: To determine whether gonadotropin-releasing hormone (GnRH) antagonist protocol can improve cumulative live birth rates (CLBRs) and shorten the time to live birth (TTLB) in unselected patients compared with progestin-primed ovarian stimulation (PPOS).

Design: A propensity score-matched retrospective cohort study design.

Setting: Tertiary-care academic medical center.

Patient(s): A total of 6,520 women with infertility aged 20-50 years were included.

Intervention(s): Patients underwent either the GnRH antagonist protocol (n = 5,004) or PPOS (n = 1,516) on the basis of the assessment of the attending physicians. One-to-one propensity score matching was performed with a caliper of 0.02. Women who were not matched were excluded from the analyses.

Main Outcome Measure(s): The CLBR of which the ongoing status had to be achieved within 22 months from the day of ovarian stimulation and TTLB.

Result(s): Each group comprised 1,424 couples after propensity score matching, and the baseline demographic characteristics of the couples after matching were comparable between the 2 groups. The cycle cancellation rate was significantly lower in the GnRH antagonist group than in the PPOS group (12.9% vs. 19.6%). The implantation rate, clinical pregnancy rate, ongoing pregnancy rate, and live birth rate per transfer were comparable between the 2 groups. However, CLBRs after 1 complete IVF cycle were significantly higher in the GnRH antagonist group than in the PPOS group (36.0% vs. 32.2%; Risk ratio = 1.12; 95% confidence interval [CI], 1.01-1.24). The average TTLB was significantly shorter in the GnRH antagonist group than in the PPOS group (9.3 months vs. 12.4 months). Using the Kaplan-Meier analysis, the cumulative incidence of ongoing pregnancy leading to live birth was significantly higher in the GnRH antagonist group than in the PPOS group (85.1% vs. 66.1%, Log-rank test). A Cox proportional hazard model revealed that women who underwent the antagonist protocol were 2.32 times more likely to achieve a live birth than those who used PPOS (hazard ratio [HR] = 2.32; 95% CI, 1.91-2.83). Subgroup analysis revealed that women who used the antagonist protocol were more likely to achieve a live birth than women who used PPOS across the 3 antral follicle count (AFC) strata (AFC ≤ 5, AFC 6-15, and AFC > 15), 2 age strata (<35 and ≥35 years), and first cycle or repeated cycle. The difference was greatest among women whose AFC was ≤5 and who were aged ≥35 years, effectively becoming smaller in the group with high ovarian reserve and younger age.

Conclusion(s): In unselected women undergoing IVF, the GnRH antagonist protocol was associated with a higher CLBR and a shorter TTLB compared with PPOS.
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http://dx.doi.org/10.1016/j.fertnstert.2022.06.012DOI Listing
August 2022

A comparison of eating safety and quality of live and dead freshwater crayfish (Procambarus clarkii) at different stages.

Authors:
Yidan Chen Hong Chen Fangshuo Gong Fang Yang Qixing Jiang Yanshun Xu Wenshui Xia

Food Res Int 2022 Sep 9;159:111630. Epub 2022 Jul 9.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:

The risk of Procambarus clarkii eating safety attracts consumers' big concern, but it has not been addressed properly. Therefore, this study was aimed to investigate eating safety and quality of live and dead Procambarus clarkii at different stages by total volatile basic nitrogen (TVB-N), biogenic amines (BAs), total aerobic plate counts (TPC) and microbiota. The results showed that in live Procambarus clarkii, TVB-N and TPC values were below the limit despite vitality, while cadaverine in gills, intestines, and glands (GIG) exceeded in articulo-mortis Procambarus clarkii. For the dead, it showed that Procambarus clarkii posed a high risk in eating safety within one to two days after death; and BAs of high risk were putrescine and cadaverine. The dominant microorganisms threatening eating safety and quality were potentially pathogenic bacteria of Citrobacter and Acinetobacter from the environment; and spoilage bacteria of Shewanella from viscera.
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http://dx.doi.org/10.1016/j.foodres.2022.111630DOI Listing
September 2022

Fusion of CT images and clinical variables based on deep learning for predicting invasiveness risk of stage I lung adenocarcinoma.

Authors:
Haozhe Huang Dezhong Zheng Hong Chen Ying Wang Chao Chen Lichao Xu Guodong Li Yaohui Wang Xinhong He Wentao Li

Med Phys 2022 Aug 8. Epub 2022 Aug 8.

Department of Interventional Radiology, Fudan University Shanghai Cancer Center, 270 Dongan Road, Xuhui District, Shanghai, 200032, China.

Purpose: To develop a novel multimodal data fusion model by incorporating computed tomography (CT) images and clinical variables based on deep learning for predicting the invasiveness risk of stage I lung adenocarcinoma that manifests as ground-glass nodules (GGNs), and compare the diagnostic performance of it with that of radiologists.

Methods: A total of 1946 patients with solitary and histopathologically confirmed GGNs with maximum diameter less than 3 cm were retrospectively enrolled. The training dataset containing 1704 GGNs was augmented by resampling, scaling, random cropping, etc., to generate new training data. A multimodal data fusion model based on residual learning architecture and two multilayer perceptron with attention mechanism combining CT images with patient general data and serum tumor markers was built. The distance-based confidence scores (DCS) were calculated and compared among multimodal data models with different combinations. An observer study was conducted and the prediction performance of the fusion algorithms was compared with that of the two radiologists by an independent testing dataset with 242 GGNs.

Results: Among the whole GGNs, 606 GGNs are confirmed as invasive adenocarcinoma (IA) and 1340 are non-IA. The proposed novel multimodal data fusion model combining CT images, patient general data and serum tumor markers achieved the highest accuracy (88.5%), Area under a ROC curve (AUC) (0.957), F1 (81.5%), F1 (81.9%) and Matthews correlation coefficient (MCC) (73.2%) for classifying between IA and non-IA GGNs, which was even better than the senior radiologist's performance (accuracy, 86.1%). In addition, the DCSs for multimodal data suggested that CT image had a stronger influence (0.9540) quantitatively than general data (0.6726) or tumor marker (0.6971).

Conclusion: This study demonstrated that the feasibility of integrating different types of data including CT images and clinical variables, and the multimodal data fusion model yielded higher performance for distinguishing IA from non-IA GGNs. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/mp.15903DOI Listing
August 2022

Foot-and-mouth disease virus VP1 promotes viral replication by regulating the expression of chemokines and GBP1.

Authors:
Li Yang Hong Chen Liqing Liu Jingjing Song Tian Feng Yihan Li Chao Shen Lingbao Kong Xiu Xin

Front Vet Sci 2022 22;9:937409. Epub 2022 Jul 22.

Nanchang City Key Laboratory of Animal Virus and Genetic Engineering, Nanchang, China.

Foot-and-mouth disease virus (FMDV) is an acute, highly contagious, and economically destructive pathogen of vesicular disease that affects domestic and wild cloven-hoofed animals. The FMDV VP1 protein is an important part of the nucleocapsid and plays a significant role during FMDV infection. However, the signal pathways mediated by VP1 in the life cycle of FMDV and the related mechanisms are not yet fully understood. Here, we performed RNA-seq to compare gene expression profiles between pCAGGS-HA-VP1 transfected PK-15 cells and pCAGGS-HA (empty vector) transfected PK-15 cells. The results showed 5,571 genes with significantly different expression levels, of which 2,981 were up-regulated and 2,590 were down-regulated. GO enrichment analysis showed that 51 GO terms were significantly enriched in cell components including protein complex, membrane and organelle part. KEGG enrichment analysis showed 11 KEGG pathways were significantly enriched which were mainly related to the immune system, infectious viral disease, and signal transduction. Among the up-regulated genes, the chemokines such as CCL5, CXCL8, and CXCL10 in turn promoted FMDV replication. In contrast, GBP1, an interferon-stimulated gene that was suppressed by VP1 and FMDV, could effectively inhibit FMDV replication. Our research provides a comprehensive overview of the response of host cells to VP1 protein and a basis for further research to understand the roles of VP1 in FMDV infection including the genes involved in FMDV replication.
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http://dx.doi.org/10.3389/fvets.2022.937409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353127PMC
July 2022

The impact of intellectual property protection on the development of digital economy and regional entrepreneurial activity: Evidence from small and medium enterprises.

Authors:
Hong Chen

Front Psychol 2022 22;13:951696. Epub 2022 Jul 22.

Shanghai International College of Intellectual Property, Tongji University, Shanghai, China.

Substantial intellectual property management (IPM) is vital in retaining competitive advantage and managing outbound open innovation (OI), which may enhance an organization's commercialization and entrepreneurial performance. Thus, the objective of this study was to develop an understanding of the impact of intellectual property protection on the development of the digital economy, regional entrepreneurial activity, and explore how IPM can enhance the entrepreneurial performance (EP) through open innovation (OI) and commercialization performance (CP) in the context of small and medium enterprises (SMEs) in Mainland China. Our study also shows how open innovation model constraints (OIMC) and information communication technology (ICT) enhance the performance. Using the organizational performance theory, we developed our research framework and collected usable data from 530 respondents from the management of SMEs in Mainland China. Data analyses were performed using SPSS, and structural equation modeling was performed using Amos 24 to test the hypothesis. Our results highlighted the significant effect of IPM on OI, CP, and EP. This study suggests various conclusions, stressing the mediating function of CP in improving EP and the direct and indirect effects of OI and CP on EP. This study also emphasizes that business managers need to ensure collaboration among SMEs since it is the best strategy to use each other's resources, including OI ideas, to improve the EP, and it should be done utilizing ICT.
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http://dx.doi.org/10.3389/fpsyg.2022.951696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355399PMC
July 2022

Critical Roles of G3BP1 in Red-Spotted Grouper Nervous Necrosis Virus-Induced Stress Granule Formation and Viral Replication in Orange-Spotted Grouper ().

Authors:
Mengshi Sun Siting Wu Shaozhu Kang Jiaming Liao Luhao Zhang Zhuqing Xu Hong Chen Linting Xu Xin Zhang Qiwei Qin Jingguang Wei

Front Immunol 2022 22;13:931534. Epub 2022 Jul 22.

College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.

Viral infection causes changes in the internal environment of host cells, and a series of stress responses are generated to respond to these changes and help the cell survive. Stress granule (SG) formation is a type of cellular stress response that inhibits viral replication. However, the relationship between red-spotted grouper nervous necrosis virus (RGNNV) infection and SGs, and the roles of the SG marker protein RAS GTPase-activating protein (SH3 domain)-binding protein 1 (G3BP1) in viral infection remain unclear. In this study, RGNNV infection induced grouper spleen (GS) cells to produce SGs. The SGs particles co-located with the classic SG marker protein eIF3η, and some SGs depolymerized under treatment with the translation inhibitor, cycloheximide (CHX). In addition, when the four kinases of the eukaryotic translation initiation factor 2α (eIF2α)-dependent pathway were inhibited, knockdown of HRI and GCN2 with small interfering RNAs and inhibition of PKR with 2-aminopurine had little effect on the formation of SGs, but the PERK inhibitor significantly inhibited the formation of SGs and decreased the phosphorylation of eIF2α. G3BP1 of named as EcG3BP1) encodes 495 amino acids with a predicted molecular weight of 54.12 kDa and 65.9% homology with humans. Overexpression of EcG3BP1 inhibited the replication of RGNNV by up-regulating the interferon and inflammatory response, whereas knockdown of EcG3BP1 promoted the replication of RGNNV. These results provide a better understanding of the relationship between SGs and viral infection in fish.
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http://dx.doi.org/10.3389/fimmu.2022.931534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354888PMC
August 2022

A fluorescence biosensor based on double-stranded DNA and a cationic conjugated polymer coupled with exonuclease III for acrylamide detection.

Authors:
Yufei Liu Suyu Meng Jingjing Qin Ruiying Zhang Ningning He Yaoyao Jiang Hong Chen Na Li Ying Zhao

Int J Biol Macromol 2022 Aug 4;219:346-352. Epub 2022 Aug 4.

School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, PR China; Xinxiang Key Laboratory of Clinical Psychopharmacology, Xinxiang Medical University, Xinxiang 453003, PR China.

As a toxic substance on human health produced in food thermal treatment, simple analytical approaches are highly desired for the detection of acrylamide (ACR) in foods. With the aid of exonuclease III (Exo III), a simple fluorescence sensor was proposed based on carboxyfluorescein-labeled double-stranded DNA (FAM-dsDNA) and a cationic conjugated polymer (PFP). Fluorescence resonance energy transfer (FRET) efficiency between FAM and PFP was changed with and without ACR. When ACR was present, ACR and single-stranded DNA (P1, ssDNA) formed an adduct, allowing free FAM-labeled complementarity strand DNA (P2, FAM-csDNA) to appear in the solution and avoiding the digestion of P2 by Exo III. After the addition of PFP, the interaction of PFP and FAM induced strong FRET. Under optimized conditions, ACR was detected with a limit of detection (LOD) of 0.16 μM. According to this biosensor, a LOD of 1.3 μM in water extract samples was observed with a good recovery rate (95-110 %).
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.251DOI Listing
August 2022

Photo-assisted reductive cleavage and catalytic hydrolysis-mediated persulfate activation by mixed redox-couple-involved CuFeS for efficient trichloroethylene oxidation in groundwater.

Authors:
Junyi Huang Yuanhao Zhou Shimao Deng Yangzi Shangguan Ranhao Wang Qiuyue Ge Xuezhen Feng Zhigang Yang Yongfei Ji Ting Fan Baiyang Chen Boqiang Li Chunmiao Zheng Xijun Hu Hong Chen

Water Res 2022 Jul 21;222:118885. Epub 2022 Jul 21.

Shenzhen Key Laboratory of Interfacial Science and Engineering of Materials, State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address:

Persulfate (PS, SO) activation through transition metal sulfides (TMS) has gained increasing attention since it can decompose a wide variety of refractory halogenated organic compounds in groundwater and wastewater. However, the processes of PS activation by TMS and particularly the formation of •OH radical under anoxic and acidic conditions (pH ∼2.8) remain elusive. Herein, by employing mixed redox-couple-involved chalcopyrite (CuFeS) (150 mg/L) nanoparticles for PS (3.0 mM) activation, 96% of trichloroethylene was degraded within 120 min at pH 6.8 under visible light irradiation. The combination of experimental studies and theoretical calculations suggested that the Cu(I)/Fe(III) mixed redox-couple in CuFeS plays a crucial role to activate PS. Cu(I) acted as an electron donor to transfer electron to Fe(III), then Fe(III) served as an electron transfer bridge as well as a catalytic center to further donate this received electron to the O-O bond of PS, thus yielding SO for trichloroethylene oxidation. Moreover, for the first time, •OH radicals were found to form from the catalytic hydrolysis of PS onto CuFeS surface, where SO anion was hydrolyzed to yield HO and these ensuing HO were further transformed into •OH radicals via photoelectron-assisted O-O bond cleavage step. Our findings offer valuable insights for understanding the mechanisms of PS activation by redox-couple- involved TMS, which could promote the design of effective activators toward PS decomposition for environmental remediation.
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http://dx.doi.org/10.1016/j.watres.2022.118885DOI Listing
July 2022

Orbitofrontal neural dissociation of healthy and unhealthy food reward sensitivity in normal-weight binge eaters.

Authors:
Wei Li Ximei Chen Yijun Luo Lin Luo Hong Chen

Psychiatry Res 2022 Jul 22;316:114736. Epub 2022 Jul 22.

Key Laboratory of Cognition and Personality (SWU), Ministry of Education, Chongqing 400715, China; Faculty of Psychology, Southwest University, Chongqing 400715, China; Research Center of Psychology and Social Development, Chongqing 400715, China. Electronic address:

Objective: The orbitofrontal cortex (OFC) has been repeatedly found to play an important role in food reward processing and binge eating (BE) episodes. However, most studies have focused mainly on reward-related neural alterations in clinical binge eating patients, with little consideration of preclinical individuals with BE that are more likely to develop from non-clinical individuals to clinical patients in the future. This study aimed to examine whether preclinical binge eaters exhibited OFC-related resting-state functional connectivity (rsFC) in the context of food reward.

Method: Binge eaters (BE group, n = 28) and healthy controls (HCs, n = 28) matched for age and body mass index (BMI) underwent rs-fMRI scans and completed self-reported assessment of BE symptoms. Food reward sensitivity was measured using the modified food incentive delay task. Analysis of covariance was used to assess the between-group differences in the medial and lateral OFC (a priori selected regions of interest) connectivity patterns in the context of food reward, while controlling for age, sex, and BMI.

Results: Lower unhealthy food (UF) reward sensitivity was significantly associated with stronger inverse OFC-putamen connectivity for HCs, while the BE group showed no association between UF reward sensitivity and the OFC-putamen connectivity. Higher healthy food (HF) reward sensitivity in the BE group was significantly correlated with stronger positive OFC-middle frontal gyrus and OFC-inferior parietal gyrus connectivity, while the opposite was found for HCs.

Conclusions: Binge eaters showed less functional synchrony within reward regions contributing to the UF reward sensitivity, but enhanced neural interactions between reward and inhibitory control regions correlated with the HF reward sensitivity. These novel findings may demonstrate the potential orbitofrontal neural dissociation of unhealthy and healthy food reward sensitivity in normal-weight binge eaters.
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http://dx.doi.org/10.1016/j.psychres.2022.114736DOI Listing
July 2022

Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse.

Authors:
Haojie Li Hong Chen Xiangzheng Zhang Yaling Qi Bing Wang Yiqiang Cui Jie Ren Yichen Zhao Yu Chen Tianyu Zhu Yue Wang Liping Yao Yueshuai Guo Hui Zhu Yan Li Chenghao Situ Xuejiang Guo

Cell Mol Life Sci 2022 Aug 5;79(8):467. Epub 2022 Aug 5.

State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, 211166, China.

Meiosis, a highly conserved process in organisms from fungi to mammals, is subjected to protein phosphorylation regulation. Due to the low abundance of phosphorylation, there is a lack of systemic characterization of phosphorylation regulation of meiosis in mammals. Using the phosphoproteomic approach, we profiled large-scale phosphoproteome of purified primary spermatocytes undergoing meiosis I, and identified 14,660 phosphorylation sites in 4419 phosphoproteins. Kinase-substrate phosphorylation network analysis followed by in vitro meiosis study showed that CDK9 was essential for meiosis progression to metaphase I and had enriched substrate phosphorylation sites in proteins involved in meiotic cell cycle. In addition, histones and epigenetic factors were found to be widely phosphorylated. Among those, HASPIN was found to be essential for male fertility. Haspin knockout led to misalignment of chromosomes, apoptosis of metaphase spermatocytes and a decreased number of sperm by deregulation of H3T3ph, chromosomal passenger complex (CPC) and spindle assembly checkpoint (SAC). The complicated protein phosphorylation and its important regulatory functions in meiosis indicated that in-depth studies of phosphorylation-mediated signaling could help us elucidate the mechanisms of meiosis.
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http://dx.doi.org/10.1007/s00018-022-04507-8DOI Listing
August 2022

Efficacy, safety and immunogenicity of hexavalent rotavirus vaccine in Chinese infants.

Authors:
Zhiwei Wu Qingliang Li Yan Liu Huakun Lv Zhaojun Mo Fangjun Li Qingchuan Yu Fei Jin Wei Chen Yong Zhang Teng Huang Xiaosong Hu Wei Xia Jiamei Gao Haisong Zhou Xuan Bai Yueyue Liu Zhenzhen Liang Zhijun Jiang Yingping Chen Jiuwei Zhang Jialiang Du Biao Yang Bo Xing Yantao Xing Ben Dong Qinghai Yang Chen Shi Tingdong Yan Bo Ruan

Virol Sin 2022 Aug 1. Epub 2022 Aug 1.

National Engineering Technology Research Center for Combined Vaccines, Wuhan Institute of Biological Product Co., Ltd., Wuhan, 430207, China.

A randomized, double-blind, placebo-controlled multicenter trial was conducted in healthy Chinese infants to assess the efficacy and safety of a hexavalent live human-bovine reassortant rotavirus vaccine (HRV) against rotavirus gastroenteritis (RVGE). A total of 6400 participants aged 6-12 weeks were enrolled and randomly assigned to either HRV (n = 3200) or placebo (n = 3200) group. All the subjects received three oral doses of vaccine four weeks apart. The vaccine efficacy (VE) against RVGE caused by rotavirus serotypes contained in HRV was evaluated from 14 days after three doses of administration up until the end of the second rotavirus season. VE against severe RVGE, VE against RVGE hospitalization caused by serotypes contained in HRV, and VE against RVGE, severe RVGE, and RVGE hospitalization caused by naturally infecting any serotype of rotavirus were also investigated. All adverse events (AEs) were collected for 30 days after each dose. Serious AEs (SAEs) and intussusception cases were collected during the entire study. Our data showed that VE against RVGE caused by serotypes contained in HRV was 69.21% (95%CI: 53.31-79.69). VE against severe RVGE and VE against RVGE hospitalization caused by serotypes contained in HRV were 91.36% (95%CI: 78.45-96.53) and 89.21% (95%CI: 64.51-96.72) respectively. VE against RVGE, severe RVGE, and RVGE hospitalization caused by naturally infecting any serotype of rotavirus were 62.88% (95%CI: 49.11-72.92), 85.51% (95%CI: 72.74-92.30) and 83.68% (95%CI: 61.34-93.11). Incidences of AEs from the first dose to one month post the third dose in HRV and placebo groups were comparable. There was no significant difference in incidences of SAEs in HRV and placebo groups. This study shows that this hexavalent reassortant rotavirus vaccine is an effective, well-tolerated, and safe vaccine for Chinese infants.
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http://dx.doi.org/10.1016/j.virs.2022.07.011DOI Listing
August 2022

Association of Inhaled Corticosteroids With All-cause Death Risk in COPD Patients: A Meta-analysis of Sixty Randomized Controlled Trials.

Authors:
Hong Chen Zheng-Xu Deng Jian Sun Qiang Huang Lan Huang Yong-Hong He Chunlan Ma Ke Wang

Chest 2022 Jul 31. Epub 2022 Jul 31.

Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, China. Electronic address:

Background: Inhaled corticosteroids (ICSs) have been widely used in the maintenance therapy of COPD. However, whether inhaled therapy containing ICSs can reduce the all-cause death risk and the possible benefited patient subgroups are unclear.

Research Question: Does inhaled therapy containing ICSs reduce the all-cause death risk in COPD patients compared with other inhaled therapy not containing ICSs?

Study Design And Methods: We searched PubMed, Cochrane Library, Embase, and Clinical Trials.gov for relevant randomized clinical trials (RCTs). Pooled results were calculated using Peto odds ratios (Peto ORs) with corresponding 95% CIs.

Results: Sixty RCTs enrolling 103,034 patients were analyzed. Inhaled therapy containing ICSs (Peto OR, 0.90; 95% CI, 0.84-0.97), especially triple therapy (Peto OR, 0.73; 95% CI, 0.59-0.91), was associated with a reduction in the cause-death risk among COPD patients when compared to inhaled therapy without ICSs. Subgroup analyses revealed that treatment duration ＞ 6 months (Peto OR, 0.90; 95% CI, 0.83-0.97), medium-dose (Peto OR, 0.71; 95% CI, 0.56-0.91)/low-dose ICSs (Peto OR, 0.88; 95% CI, 0.79-0.97), and budesonide (Peto OR, 0.75; 95% CI, 0.59-0.94) were involved in this association. The predictors of this association included eosinophil counts ≥ 200/μL or percentage ≥ 2%, documented history of moderate and severe exacerbations in the previous year ≥ 2, GOLD stage III or IV, age ＜ 65 years old, and BMI ≥ 25, among which eosinophil counts ≥ 200/μL (Peto OR, 0.58; 95% CI, 0.36-0.95) was the strongest predictor.

Interpretation: Inhaled therapy containing ICSs, especially triple therapy, longer than 6 months, was associated with a reduction in the cause-death risk in COPD patients. The predictors of this association included medication factors and patient characteristics, among which eosinophil counts ≥ 200/μL was the strongest predictor.

Trial Registry: PROSPERO: CRD42022304725; https://www.crd.york.ac.uk/PROSPERO/.
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http://dx.doi.org/10.1016/j.chest.2022.07.015DOI Listing
July 2022

Aging in China: Challenges and Opportunities.

Authors:
Hongman Wang Hong Chen

China CDC Wkly 2022 Jul;4(27):601-602

Chinese Center of Disease Control and Prevention, Beijing, China.

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http://dx.doi.org/10.46234/ccdcw2022.130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339359PMC
July 2022

δ-Opioid receptors in primary sensory neurons tonically restrain nociceptive input in chronic pain but do not enhance morphine analgesic tolerance.

Authors:
Daozhong Jin Hong Chen Yuying Huang Shao-Rui Chen Hui-Lin Pan

Neuropharmacology 2022 Jul 31;217:109202. Epub 2022 Jul 31.

Center for Neuroscience and Pain Research, Department of Anesthesiology and Perioperative Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA. Electronic address:

δ-Opioid receptors (DORs, encoded by the Oprd1 gene) are expressed throughout the peripheral and central nervous system, and DOR stimulation reduces nociception. Previous studies suggest that DORs promote the development of analgesic tolerance of μ-opioid receptor (MOR) agonists. It is uncertain whether DORs expressed in primary sensory neurons are involved in regulating chronic pain and MOR agonist-induced tolerance. In this study, we generated Oprd1 conditional knockout (Oprd1-cKO) mice by crossing Advillin-Cre mice with Oprd1-floxed mice. DOR expression in the dorsal root ganglion was diminished in Oprd1-cKO mice. Systemic or intrathecal injection of the DOR agonist SNC-80 produced analgesia in wild-type (WT), but not Oprd1-cKO, mice. In contrast, intracerebroventricular injection of SNC-80 produced a similar analgesic effect in WT and Oprd1-cKO mice. However, morphine-induced analgesia, hyperalgesia, or analgesic tolerance did not differ between WT and Oprd1-cKO mice. Compared with WT mice, Oprd1-cKO mice showed increased mechanical and heat hypersensitivity after nerve injury or tissue inflammation. Furthermore, blocking DORs with naltrindole increased nociceptive sensitivity induced by nerve injury or tissue inflammation in WT, but not Oprd1-cKO, mice. In addition, naltrindole potentiated glutamatergic input from primary afferents to spinal dorsal horn neurons increased by nerve injury or CFA in WT mice; this effect was absent in Oprd1-cKO mice. Our findings indicate that DORs in primary sensory neurons are critically involved in the analgesic effect of DOR agonists but not morphine-induced analgesic tolerance. Presynaptic DORs at primary afferent central terminals constitutively inhibit inflammatory and neuropathic pain by restraining glutamatergic input to spinal dorsal horn neurons.
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http://dx.doi.org/10.1016/j.neuropharm.2022.109202DOI Listing
July 2022

Regulates Bovine Myoblasts Proliferation and Differentiation via the miR-411a/ Axis.

Authors:
Zhaoxin Yang Chengchuang Song Rui Jiang Yongzhen Huang Xianyong Lan Chuzhao Lei Xinglei Qi Chunlei Zhang Bizhi Huang Hong Chen

J Agric Food Chem 2022 Aug 2. Epub 2022 Aug 2.

College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Animal Genetics, Breeding and Reproduction, Yangling, Shaanxi 712100, China.

Circular RNA (circRNA) is endogenous noncoding RNA found throughout the eukaryotic genome. It regulates several biological activities at the transcription or post-transcription level. However, the underlying function of circRNA in bovine skeletal muscle development remains unknown. Here, we identified a novel circRNA, , and investigated its function and mechanism on the proliferation and differentiation of bovine myoblasts. At the molecular and cellular levels, could promote bovine myoblasts proliferation and inhibit differentiation. Mechanistically, is expressed in the cytoplasmic of myoblast and was enriched by protein Ago2. acts as a competing endogenous RNA that sponges miR-411a and alleviates the inhibitory effect on its target gene, . miR-411a and were also involved in regulating bovine myoblast proliferation and differentiation. These findings suggest that functions as a competing endogenous RNA and regulates bovine myoblast proliferation and differentiation through the miR-411a/ axis.
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http://dx.doi.org/10.1021/acs.jafc.1c08167DOI Listing
August 2022

Ellagic Acid Alters Muscle Fiber-Type Composition and Promotes Mitochondrial Biogenesis through the AMPK Signaling Pathway in Healthy Pigs.

Authors:
Huawei Li Xiaoling Chen Daiwen Chen Bing Yu Jun He Ping Zheng Yuheng Luo Hui Yan Hong Chen Zhiqing Huang

J Agric Food Chem 2022 Aug 2;70(31):9779-9789. Epub 2022 Aug 2.

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.

Ellagic acid (EA), because of its remarkable health-promoting ability, has aroused widespread interest in the fields of nutrition and medicine. However, no reports showed that EA regulates mitochondrial biogenesis as well as muscle fiber-type composition in pigs. Our study found that dietary 75 and 150 mg/kg EA obviously augmented the slow myosin heavy chain (MyHC) protein level, the number of slow-twitch muscle fibers, and the activity of malate dehydrogenase (MDH) in the longissimus thoracis (LT) muscle of growing-finishing pigs. In contrast, dietary 75 and 150 mg/kg EA decreased the fast MyHC level, the number of fast-twitch muscle fibers, and the activity of lactate dehydrogenase (LDH) in the LT muscle. In addition, our further study found that dietary 75 and 150 mg/kg EA promoted the mitochondrial DNA (mtDNA) content, the mRNA expressions of ATP synthase (), mtDNA transcription factor A (), AMP-activated protein kinase α1 (α), peroxisome proliferator-activated receptor-γ coactivator-1α (α) and sirtuin 1 (), and the level of phospho-LKB1 (P-LKB1), phospho-AMPK (P-AMPK), Sirt1, and PGC-1α in the LT muscle. , 5, 10, and 20 μmol/L EA treatment upregulated the level of slow MyHC, but only 10 μmol/L EA treatment decreased fast MyHC protein expression in porcine skeletal muscle satellite cells (PSCs). In addition, our data again found that 10 μmol/L EA treatment promoted the mtDNA content, the mRNA levels of , mitochondrial transcription factor b1 (), citrate synthase (), α, α, and , and the protein expressions of P-AMPK, P-LKB1, PGC-1α, and Sirt1 in PSCs. What is more, inhibition of the AMPK signaling pathway by AMPKα1 siRNA significantly eliminated the improvement of EA on muscle fiber-type composition as well as the mtDNA content in PSCs. In conclusion, EA altered muscle fiber-type composition and promoted mitochondrial biogenesis through the AMPK signaling pathway.
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http://dx.doi.org/10.1021/acs.jafc.2c04108DOI Listing
August 2022

The strigolactone receptor SlDWARF14 plays a role in photosynthetic pigment accumulation and photosynthesis in tomato.

Authors:
Zhifei Li Ying Pi Changsheng Zhai Dong Xu Wenyao Ma Hong Chen Yi Li Han Wu

Plant Cell Rep 2022 Jul 30. Epub 2022 Jul 30.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Key Message: Tomato DWARF14 regulates the development of roots, shoot branches and leaves, and also plays a role in photosynthetic pigment accumulation and photosynthetic capacity. Strigolactones (SLs) are a novel class of plant hormones. DWARF14 (D14) is the only SL receptor identified to date, but it is not functionally analyzed in tomato (Solanum lycopersicum). In the present study, we identified the potential SL receptor in tomato by bioinformatic analysis, which was designated as SlD14. SlD14 was expressed in roots, stems, flowers and developing fruits, with the highest expression level in leaves. sld14 mutant plants produced by the CRISPR/Cas9 system displayed reduced plant height and root biomass, increased shoot branching and altered leaf shape comparing with WT plants. The cytokinin biosynthetic gene ISOPENTENYLTRANSFERASE 3 (SlIPT3), auxin biosynthetic genes FLOOZY (SlFZY) and TRYPTOPHAN AMINOTRANSFERASE RELATED 1 (SlTAR1) and several auxin transport genes SlPINs, which are involved in branch formation, showed higher expression levels in the sld14 plant stem. In addition, sld14 plants exhibited light-green leaves, reduced chlorophyll and carotenoid contents, abnormal chloroplast structure and reduced photosynthetic capacity. Transcriptomic analysis showed that the transcript levels of six chlorophyll biosynthetic genes, three carotenoid biosynthetic genes and numerous chlorophyll a/b-binding protein genes were decreased in sld14 plants. These results suggest that tomato SL receptor gene SlD14 not only regulates the development of roots, shoot branches and leaves, but also plays a role in regulating photosynthetic pigment accumulation and photosynthetic capacity.
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http://dx.doi.org/10.1007/s00299-022-02908-4DOI Listing
July 2022

In Vivo Distribution and Therapeutic Efficacy of Radioiodine-Labeled pH-Low Insertion Peptide Variant 3 in a Mouse Model of Breast Cancer.

Authors:
Min Zhang Yue Xi Hong Chen Wangxi Hai Biao Li

Mol Imaging 2022 4;2022:7456365. Epub 2022 Jul 4.

Department of Nuclear Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Purpose: Extracellular acidity is a marker of highly aggressive breast cancer (BC). pH-low insertion peptides (pHLIPs) target the acidic tumor microenvironment. This study evaluates the distribution and therapeutic efficacy of radioiodine-labeled pHLIP variant 3 (Var3) in a mouse model of BC.

Methods: The binding of fluorescein isothiocyanate (FITC)- or radioiodine-125 (I) labeled Var3-pHLIP to MDA-MB-231, 4T1, and SK-BR-3 BC cell lines under different pH values was evaluated in vitro. The distribution of I-labeled Var3-pHLIP and wild-type- (WT-) pHLIP in tumor-bearing mice was analyzed in vivo using micro-SPECT/CT imaging. The therapeutic efficacy of radioiodine-131 (I)-labeled Var3-pHLIP in MDA-MB-231 xenografts was evaluated by relative tumor volume measurement and immunohistochemical analysis.

Results: The binding ability of FITC- or I-labeled Var3-pHLIP to tumor cells increased with the decrease in pH. The tumor-to-background ratio of I-Var3-pHLIP in BC xenografts showed the best imaging contrast at 24 h or 48 h postinjection. The uptake of I-Var3-pHLIP in MDA-MB-231 xenografts at 2 h postinjection was significantly higher than that of I-WT-pHLIP (3.76 ± 0.37 vs. 2.87 ± 0.60%ID/g, = 0.046). The relative tumor volume in MDA-MB-231 xenografts was significantly lower in the I-Var3-pHLIP-treated group than in the groups treated with Var3-pHLIP ( = 0.027), I ( = 0.001), and saline ( < 0.001). The I-Var 3-pHLIP group presented a lower expression of Ki67 and a higher expression of caspase 3.

Conclusion: Radioiodine-labeled Var3-pHLIP effectively targeted BC cells in an acidic environment and inhibited the growth of MDA-MB-231 xenografts by ionizing radiation.
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http://dx.doi.org/10.1155/2022/7456365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281440PMC
July 2022

Knowing what you feel: Inferior frontal gyrus-based structural and functional neural patterns underpinning adaptive body awareness.

Authors:
Lin Luo Mingyue Xiao Yijun Luo Haijing Yi Debo Dong Yong Liu Ximei Chen Wei Li Hong Chen

J Affect Disord 2022 Jul 25. Epub 2022 Jul 25.

Key Laboratory of Cognition and Personality (SWU), Ministry of Education, Chongqing, China; Department of Psychology, Southwest University, Chongqing, China. Electronic address:

Background: Heightened body awareness (BA) is conducive for increasing understanding of bodily state and improves individuals' health and well-being. Although there has been cumulative research concentrating on the self-perceived tendency to focus on negatively valenced interoceptive sensations, the specific structural and functional neural patterns underlying BA and their role in the relationship between BA and individual well-being remain unclear.

Methods: Voxel-based morphometry and whole brain functional connectivity analyses were conducted to examine the structural and functional neural patterns, respectively, in 686 healthy subjects. BA and subjective well-being were assessed using questionnaires.

Results: BA was inversely related to gray matter volume of the right inferior frontal gyrus, opercular part (IFGoperc). Higher BA was correlated with enhanced IFGoperc-precuneus and IFGoperc-anterior supramarginal gyrus connectivities, and with decreased IFGoperc-lateral occipital cortex and IFGoperc-medial frontal cortex connectivities. The inferior frontal gyrus, triangular part (in the fronto-parietal task control network) acted as the hub that linked the sensory/somatomotor network, the default mode network, and the dorsal and ventral attention network. The IFGoperc-precuneus connectivity moderated the association between BA and subjective well-being.

Limitations: We were unable to rank all the networks by their relative importance, because the absolute weighted value in each module was not calculated.

Conclusion: Our findings demonstrated that BA was reflected by specific neural patterns mainly involved in cognitive-affective control, attentional and self-referential processing, as well as multisensory integration, which could offer some references for current therapies (e.g., mindfulness, yoga training) that are dedicated to solving health problems and improving individual well-being.
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http://dx.doi.org/10.1016/j.jad.2022.07.051DOI Listing
July 2022

Targeting PDE4B (Phosphodiesterase-4 Subtype B) for Cardioprotection in Acute Myocardial Infarction via Neutrophils and Microcirculation.

Authors:
Qing Wan Chuansheng Xu Liyuan Zhu Yuze Zhang Zekun Peng Hong Chen Haojie Rao Erli Zhang Hongyue Wang Fei Chu Xuan Ning Xuejian Yang Jinqing Yuan Yongjian Wu Yu Huang Shengshou Hu De-Pei Liu Miao Wang

Circ Res 2022 Jul 28:CIRCRESAHA122321365. Epub 2022 Jul 28.

State Key Laboratory of Cardiovascular Disease (Q.W., C.X., L.Z., Y.Z., Z.P., H.C., H.R., F.C., X.N., X.Y., S.H., M.W.), Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Timely and complete restoration of blood flow is the most effective intervention for patients with acute myocardial infarction. However, the efficacy is limited by myocardial ischemia-reperfusion (MI/R) injury. PDE4 (phosphodiesterase-4) hydrolyzes intracellular cAMP and it has 4 subtypes A-D. This study aimed to delineate the role of PDE4B (phosphodiesterase-4 subtype B) in MI/R injury.

Methods: Mice were subjected to 30-minute coronary artery ligation, followed by 24-hour reperfusion. Cardiac perfusion was assessed by laser Doppler flow. Vasomotor reactivities were determined in mouse and human coronary (micro-)arteries.

Results: Cardiac expression of PDE4B, but not other PDE4 subtypes, was increased in mice following reperfusion. PDE4B was detected primarily in endothelial and myeloid cells of mouse and human hearts. PDE4B deletion strikingly reduced infarct size and improved cardiac function 24-hour or 28-day after MI/R. PDE4B in bone marrow-derived cells promoted MI/R injury and vascular PDE4B further exaggerated this injury. Mechanistically, PDE4B-mediated neutrophil-endothelial cell interaction and PKA (protein kinase A)-dependent expression of cell adhesion molecules, neutrophil cardiac infiltration, and release of proinflammatory cytokines. Meanwhile, PDE4B promoted coronary microcirculatory obstruction and vascular permeability in MI/R, without affecting flow restriction-induced thrombosis. PDE4B blockade increased flow-mediated vasodilatation and promoted endothelium-dependent dilatation of coronary arteries in a PKA- and nitric oxide-dependent manner. Furthermore, postischemia administration with piclamilast, a PDE4 pan-inhibitor, improved cardiac microcirculation, suppressed inflammation, and attenuated MI/R injury in mice. Incubation with sera from patients with acute myocardial infarction impaired acetylcholine-induced relaxations in human coronary microarteries, which was abolished by PDE4 inhibition. Similar protection against MI/R-related coronary injury was recapitulated in mice with PDE4B deletion or inhibition, but not with the pure vasodilator, sodium nitroprusside.

Conclusions: PDE4B is critically involved in neutrophil inflammation and microvascular obstruction, leading to MI/R injury. Selective inhibition of PDE4B might protect cardiac function in patients with acute myocardial infarction designated for reperfusion therapy.
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http://dx.doi.org/10.1161/CIRCRESAHA.122.321365DOI Listing
July 2022

Identification and Validation of an m7G-Related lncRNAs Signature for Prognostic Prediction and Immune Function Analysis in Endometrial Cancer.

Authors:
Jiani Sun Li Li Hong Chen Lei Gan Xiaoqing Guo Jing Sun

Genes (Basel) 2022 Jul 22;13(8). Epub 2022 Jul 22.

Department of Gynecology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China.

Background: N7-methylguanosine is a novel kind of internal modification that is widespread in human mRNA. The relationship between m7G-related lncRNAs (MRL) and endometrial cancer remains unknown. The aim of our study is to explore a predictive prognosis MRL signature in endometrial cancer and identify the underlying biological mechanism.

Methods: We obtained RNA-seq profiles, clinical data, and information on somatic mutations from the TCGA database and obtained m7G-related genes from a previous study. MRLs were identified through a co-expression network. The prognostic model was constructed based on 10 m7G-related lncRNAs. Differentially expressed genes between low- and high-risk groups were identified for further analysis, consisting of functional enrichment analysis, immune function analysis, somatic mutation analysis, and potential drugs exploration.

Results: We constructed a 10-MRLs signature. According to the risk score, the signature was classified into high- and low-risk groups. The signature had a reliable capacity for predicting the prognosis of endometrial cancer patients. The findings about differentially expressed genes were also of great significance for therapeutic treatments for endometrial cancer and gave novel insights into exploring the underlying molecular mechanism.

Conclusion: The prognostic model based on 10 MRLs is a reliable and promising approach for predicting clinical outcomes and suggesting therapeutic methods for endometrial cancer patients.
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http://dx.doi.org/10.3390/genes13081301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330151PMC
July 2022

Potential Similarities in Sex Difference in Key Genes and Their Expression, Network, EQTL and Pathways between COVID-19 and Chronic Kidney Disease Based on Mouse Model.

Authors:
Zhuo Yu Jingyu Zhan Wei Dong Lu Lu Monica M Jablonski Lotfi Aleya Jingyu Chen Peiqing Zhang Hong Chen Weikuan Gu

J Pers Med 2022 Jul 21;12(7). Epub 2022 Jul 21.

Department of Gastroenterology, Heilongjiang Academy of Traditional Chinese Medicine, Harbin 150001, China.

COVID-19 and chronic kidney disease (CKD) share similarity in sex bias and key genes in the disease pathway of sex difference. We investigated the sex difference of molecular pathways of four key players of these two diseases using an existing large set of whole genome expression profiles from the kidneys of female and male mouse models. Our data show that there is little to no correlation at the whole genome expression level between female and male mice among these four genes. There are considerable sex differences among genes in upstream regulation, complex interaction, and downstream pathways. Snap25 and Plcb4 may play important roles in the regulation of the expression level of , , and in females. In males, Adh4 is a candidate gene for the regulation of , while Asl, Auts2, and Rabger1 are candidates for . Within the complex, directly influences the expression level of and in the female, while in the male Adam potentially has a stronger influence on than that of . Among the top 100 most related genes, only one or two genes from four key genes and 11 from the control B-Actin were found to be the same between sexes. Among the top 10 sets of genes in the downstream pathway of , only two sets are the same between the sexes. We concluded that these known key genes and novel genes in CKD may play significant roles in the sex difference in the CKD and COVID-19 disease pathways.
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http://dx.doi.org/10.3390/jpm12071190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323909PMC
July 2022

Evaluation of Occupational Health and Safety Management of Listed Companies in China's Energy Industry Based on the Combined Weight-Cloud Model: From the Perspective of FPE Information Disclosure.

Authors:
Yujie Wang Hong Chen Ruyin Long Shiyan Jiang Bei Liu

Int J Environ Res Public Health 2022 Jul 7;19(14). Epub 2022 Jul 7.

School of Economics and Management, China University of Mining and Technology, Xuzhou 221116, China.

Protecting labor safety and health and actively carrying out occupational safety and health management (OSHM) is a common need worldwide, and it is also one of the important efforts of Chinese enterprises under the background of promoting the implementation of the Healthy China strategy. Based on in-depth thinking on the current stage of OHSM, this study incorporated "management framework, management process, management effectiveness" (FPE) into an integrated framework and constructed an FPE evaluation system for enterprise OHSM. This study innovatively collected and refined FPE information from the perspective of information disclosure and used the combined weight cloud model to evaluate the occupational health and safety management level (OHSML) of 69 listed companies in China's energy industry from 2009-2019. The results showed the following. (1) The OHSML of most listed companies in China's energy industry was still at a low-end level. Among the companies that have issued relevant information reports, only 5.58% (S = 30) of the sample companies' OHSML were at an acceptable level (Level IV) or declarable level (Level V). The OHSML comprehensive evaluation level of 92.56% (S = 498) of the sample companies was between the transitional level (Level III) and the improved level (Level II). (2) During 2009-2019, although the annual OHSML of listed companies in China's energy industry showed an upward trend, the growth rate was low, and even the OHSML of some listed companies in the energy industry showed the characteristics of reduced fluctuations. (3) From the perspective of the PFT three-dimensional subsystem level of OHSM, the evaluation level of the governance framework subsystem was the highest, whereas the evaluation level of the management process subsystem and the management effectiveness subsystem were relatively low. Finally, according to the relevant results, some suggestions were proposed to improve the OHSML of listed companies in China's energy industry. These findings can provide guidance for companies to improve their OSHM performance.
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http://dx.doi.org/10.3390/ijerph19148313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318156PMC
July 2022

Comparisons of Hematological and Biochemical Profiles in Brahman and Yunling Cattle.

Authors:
Yu Yang Shuling Yang Jia Tang Gang Ren Jiafei Shen Bizhi Huang Chuzhao Lei Hong Chen Kaixing Qu

Animals (Basel) 2022 Jul 15;12(14). Epub 2022 Jul 15.

Academy of Science and Technology, Chuxiong Normal University, Chuxiong 675000, China.

Brahman cattle are tolerant to parasite challenges and heat stress. Yunling cattle are three-way hybrids that are half Brahman cattle, a fourth Murray Grey cattle and a fourth Yunnan Yellow cattle, with good beef performance. The hematological and biochemical parameters can reflect the physiology and metabolic conditions of cattle, and there are valuable indicators of production performance and adaptability that can be found by studying the cattle. To assess the health status and differences, we compared 55 hematological and biochemical parameters of 28 Brahman cattle and 65 Yunling cattle using an automatic biochemical analyzer. Our results showed that 27 hematological and biochemical indices of Brahman cattle were lower than those of Yunling cattle, whereas the other parameters were higher. There are 20 indices with significant differences that were detected between Brahman and Yunling cattle (with ≤ 0.01 or 0.01 ≤ ≤ 0.05, respectively), and no significant differences were found for other indices ( > 0.05). Based on these results, Yunling cattle may have a better physical condition than Brahman cattle, may be better at adapting to local environments, and can maintain a good production and reproduction performance. As a new breed that is half Brahman, the abilities of Yunling cattle, including adaptability, stress resistance and tolerance to crude feed, were better than Brahman cattle under the same management conditions.
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http://dx.doi.org/10.3390/ani12141813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311853PMC
July 2022

Skin-derived epithelial lining facilitates orthotopic tracheal transplantation by protecting the tracheal cartilage and inhibiting granulation hyperplasia.

Authors:
Erji Gao Pengli Wang Feifan Chen Yong Xu Qianyi Wang Hong Chen Gening Jiang Guangdong Zhou Dan Li Yi Liu Liang Duan

Biomater Adv 2022 Aug 21;139:213037. Epub 2022 Jul 21.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China. Electronic address:

Long-segment tracheal defects caused by tumours, inflammation or trauma can cause serious damage to the quality of life of patients. Although many novel neotracheas have been constructed, the therapeutic effect of orthotopic transplantation was compromised mainly because of the lack of an epithelial lining in those neotracheas. In this study, we aimed to investigate the therapeutic function of skin-derived epithelial lining for orthotopic tracheal transplantation. Strips of auricular cartilage with fixed interval were interrupted sutured on a silicone tube to mimic the cartilage rings of the native trachea. Neotrachea in the with epithelium group retained the unilateral skin as the epithelial lining in the lumen, whereas the neotrachea in the without epithelium group consisted solely of cartilage strips. After revascularized in the sternohyoid muscle, 2-cm-long tracheal defects were made and were reconstructed using these neotracheas. Our results showed that the skin-derived epithelial lining simultaneously protected the engineered tracheal cartilage and inhibited granulation hyperplasia in the tracheal lumen; further, compared with the without epithelium group, the group with epithelium showed a marked improvement in the tracheal lumen patency and the survival rate of rabbits. Our study provides a critical cue for improvements in the repair of tracheal defects via skin-derived epithelial lining and may significantly advance the clinical translation of tissue-engineered trachea.
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http://dx.doi.org/10.1016/j.bioadv.2022.213037DOI Listing
August 2022
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