Publications by authors named "Hong Chang"

1,223 Publications

  • Page 1 of 1

Factors That Influence Compliance to Long-Term Remote Ischemic Conditioning Treatment in Patients With Ischemic Stroke.

Front Neurol 2021 30;12:711665. Epub 2021 Aug 30.

Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Disease, Beijing, China.

To investigate the treatment compliance of patients with ischemic stroke to remote ischemic conditioning (RIC) and to determine the factors that influence compliance. We conducted a retrospective study of patients with ischemic stroke who were treated with RIC. Treatment compliance was determined and analyzed in patients who had received 1 year of RIC training. Factors that influenced patient compliance were also determined using univariate and multivariate regression analyses. Between March 2017 and February 2018, 91 patients were recruited into this study. The mean (±SD) age was 57.98 ± 10.76 years, and 78 (85.7%) patients were male. The baseline Kolcaba comfort scale of patients with good compliance scores were higher than those with poor compliance. The scores of the four dimensions in the scale and the total score are as follows: physiological dimensions, 15.0 (12.0,17.0) vs 17.0 (13.0,19.0); psychological dimensions, 30.0 (25.0,34.0) vs 31.0 (27.0,35.0); sociological dimensions, 20.0 (18.0,24.0) vs 21.0 (18.0,23.0); environmental dimensions, 19.0 (12.0,24.0) vs 20.0 (17.0,22.0); and total points, 82.0 (69.0,94.0) vs 91.0 (78.0,98.0). the differences between the groups were significant ( < 0.05), except for the sociological dimensions. A history of hypertension, number of follow-ups, and the physiological, psychological, and environmental dimensions of the comfort scale were related to patient compliance, out of which the number of follow-ups (Adjusted OR = 2.498, 95% confidence interval (CI) 1.257-4.964) and the physiological discomfort (Adjusted OR = 1.128, 95% CI 1.029-1.236) independently influenced compliance ( < 0.05). In patients with ischemic cerebrovascular disease who were treated with RIC, the number of follow-up visits and physiological discomfort associated with RIC treatment independently influenced patient compliance. Further studies are needed to investigate the RIC protocols and their corresponding nursing models.
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http://dx.doi.org/10.3389/fneur.2021.711665DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435569PMC
August 2021

Clinical efficacy of regional citrate anticoagulation in continuous renal replacement therapy: systematic review and meta-analysis.

Ann Palliat Med 2021 Aug;10(8):8939-8951

EICU, Qinghai Provincial People's Hospital, Xining, China.

Background: A systematic review and meta-analysis were conducted to explore the clinical efficacy and coagulation function of regional citrate anticoagulation (RCA) in continuous renal replacement therapy (CRRT) in critically ill patients, to provide an effective treatment options for CRRT in severe patients.

Methods: The English databases Embase, Medline, PubMed, Ovid, Springer, and Web of Science were searched to screen for randomized controlled trials (RCTs) on RCA in the CRRT treatment of critically ill patients published before June 1, 2020. Meta analysis using the RevMan5.3 provided by the Cochrane collaboration network. The search terms included "citrate anticoagulation", "patient in severe condition", "CRRT", "clinical effect", and "coagulation function".

Results: ten articles meeting requirements were included, comprising 1,411 subjects. Meta-analysis results showed that after treatment, total calcium/ionized calcium (totCa/ionCa) [mean difference (MD) =0.05; 95% confidence interval (CI): (-0.02 to 0.12); Z=1.31; P=0.19], prothrombin time [MD =4.51; 95% CI: (2.77, 6.24); Z=5.10; P<0.00001], activated partial thromboplastin time [MD =2.56; 95% CI: (1.17, 3.95); Z=3.61; P=0.0003], and thrombin time [MD =4.22; 95% CI: (2.07, 6.36); Z=3.85; P=0.0001] all increased. However, platelet count [MD =-5.75; 95% CI: (-8.85, -2.64); Z=3.63; P=0.0003], cystatin [MD =-0.39; 95% CI: (-0.63, -0.15); Z=3.22; P=0.001], alanine aminotransferase [MD =-17.63; 95% CI: (-20.09, -15.16); Z=14.02; P<0.00001], aspartate aminotransferase [MD =-6.49; 95% CI: (-11.94, -1.04); Z=2.33; P=0.02], creatinine [MD =-3.70; 95% CI: (-5.08, -2.32); Z=5.24; P<0.00001], and total bilirubin [MD =-3.65; 95% CI: (-5.91, -1.40); Z=3.18; P=0.001] all decreased. Except for totCa/ionCa, the differences in other indicators were not statistically significant compared with the control group.

Discussion: RCA can significantly improve the clinical efficacy and blood coagulation indicators of CRRT for severely ill patients.
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http://dx.doi.org/10.21037/apm-21-1693DOI Listing
August 2021

Comparison between early surgical treatment and conservative treatment of appendicitis in cancer patients.

ANZ J Surg 2021 Sep 3. Epub 2021 Sep 3.

Center for Colorectal Cancer, Research Institute and Hospital, National Cancer Center, Goyang, South Korea.

Backgrounds: In cancer patients, the optimal appendicitis treatment has not been established. Therefore, we aimed to determine the ideal treatment option for appendicitis in cancer patients.

Methods: This retrospective study included 185 cancer patients with acute appendicitis who were divided into the early surgical group (n = 152) involving surgery performed within 48 h following the appendicitis diagnosis or the conservative group (n = 33) involving intravenous antibiotics. We compared the appendicitis treatment efficacy between the groups.

Results: In the early surgical group, the antibiotic duration [5.5 days (4.0-8.0) vs. 17.0 days (12.5-25.0), p < 0.001] and hospital stay length [7.0 days (5.0-11.75) vs. 10.0 days (8.0-32.0), p < 0.001] were significantly shorter. Regarding pathology, 16/171 (9.4%) patients who underwent surgery exhibited appendiceal tumours. During the 1-year follow-up period, one recurrence occurred in each group [1/152 (0.7%) vs. 1/33 (3.0%), p = 0.326]. The 1-year treatment success rate was higher in the early surgical group [99.3% (151/152) vs. 42.4% (14/33), p < 0.001].

Conclusion: Early surgical treatment yielded a significantly higher success rate than conservative treatment for appendicitis in cancer patients. Surgery for appendicitis in cancer patients should be considered not only for treatment but also for pathologic confirmation.
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http://dx.doi.org/10.1111/ans.17180DOI Listing
September 2021

PRDP: Person Reidentification With Dirty and Poor Data.

IEEE Trans Cybern 2021 Sep 2;PP. Epub 2021 Sep 2.

In this article, we propose a novel method to simultaneously solve the data problem of dirty quality and poor quantity for person reidentification (ReID). Dirty quality refers to the wrong labels in image annotations. Poor quantity means that some identities have very few images (FewIDs). Training with these mislabeled data or FewIDs with triplet loss will lead to low generalization performance. To solve the label error problem, we propose a weighted label correction based on cross-entropy (wLCCE) strategy. Specifically, according to the influence range of the wrong labels, we first classify the mislabeled images into point label error and set label error. Then, we propose a weighted triplet loss (WTL) to correct the two label errors, respectively. To alleviate the poor quantity issue, we propose a feature simulation based on autoencoder (FSAE) method to generate some virtual samples for FewID. For the authenticity of the simulated features, we transfer the difference pattern of identities with multiple images (MultIDs) to FewIDs by training an autoencoder (AE)-based simulator. In this way, the FewIDs obtain richer expressions to distinguish from other identities. By dealing with a dirty and poor data problem, we can learn more robust ReID models using the triplet loss. We conduct extensive experiments on two public person ReID datasets: 1) Market-1501 and 2) DukeMTMC-reID, to verify the effectiveness of our approach.
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http://dx.doi.org/10.1109/TCYB.2021.3105970DOI Listing
September 2021

Location Sensitive Network for Human Instance Segmentation.

IEEE Trans Image Process 2021 8;30:7649-7662. Epub 2021 Sep 8.

Location is an important distinguishing information for instance segmentation. In this paper, we propose a novel model, called Location Sensitive Network (LSNet), for human instance segmentation. LSNet integrates instance-specific location information into one-stage segmentation framework. Specifically, in the segmentation branch, Pose Attention Module (PAM) encodes the location information into the attention regions through coordinates encoding. Based on the location information provided by PAM, the segmentation branch is able to effectively distinguish instances in feature-level. Moreover, we propose a combination operation named Keypoints Sensitive Combination (KSCom) to utilize the location information from multiple sampling points. These sampling points construct the points representation for instances via human keypoints and random points. Human keypoints provide the spatial locations and semantic information of the instances, and random points expand the receptive fields. Based on the points representation for each instance, KSCom effectively reduces the mis-classified pixels. Our method is validated by the experiments on public datasets. LSNet-5 achieves 56.2 mAP at 18.5 FPS on COCOPersons. Besides, the proposed method is significantly superior to its peers in the case of severe occlusion.
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http://dx.doi.org/10.1109/TIP.2021.3107210DOI Listing
September 2021

Hemophagocytosis arising during disease progression of chronic myelomonocytic leukemia.

Int J Lab Hematol 2021 Aug 31. Epub 2021 Aug 31.

Laboratory Medicine Program, Toronto General Hospital, University Health Network, University of Toronto, Toronto, ON, Canada.

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http://dx.doi.org/10.1111/ijlh.13685DOI Listing
August 2021

Clinicopathological analysis of primary carcinoid tumour of the ovary arising in mature cystic teratomas.

J Int Med Res 2021 Aug;49(8):3000605211034666

Department of Peritoneal Cancer Surgery, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Objective: To investigate the clinicopathological features, diagnosis and differential diagnosis of patients with primary ovarian carcinoid tumours arising in mature cystic teratomas.

Methods: This retrospective case series analysed the data from patients with primary ovarian carcinoid tumours arising in mature cystic teratomas.

Results: The study enrolled four patients. Histopathological analysis of the tumours identified the following subtypes: insular ( = 1), trabecular ( = 1) and strumal ( = 2). All four primary ovarian carcinoid tumours originated from a mature teratoma. The morphology of the primary ovarian carcinoids was similar to other neuroendocrine tumours. Strumal carcinoids were composed of different proportions of thyroid tissue intimately admixed with carcinoid tumour. Tumour tissue was arranged in insular and/or trabecular patterns. The nucleus of tumour cells displayed exquisite chromatin without obvious mitotic figures. Tumour tissues were positively stained for neuroendocrine markers chromogranin A, synaptophysin and CD56 to varying degrees. Strumal carcinoid tumours were cytokeratin 19 positive and thyroid transcription factor 1 negative. No recurrence or metastasis occurred during follow-up (12-71 months).

Conclusion: Primary ovarian carcinoid tumours arising in mature cystic teratomas are rare. Diagnosis and differential diagnosis should be confirmed by clinical features, histopathological characteristics and specific immunophenotyping.
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http://dx.doi.org/10.1177/03000605211034666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408898PMC
August 2021

Gibberellin Signaling Promotes the Secondary Growth of Storage Roots in .

Int J Mol Sci 2021 Aug 13;22(16). Epub 2021 Aug 13.

Department of Biology, Chungbuk National University, Cheongju 28644, Korea.

Gibberellins (GAs) are an important group of phytohormones associated with diverse growth and developmental processes, including cell elongation, seed germination, and secondary growth. Recent genomic and genetic analyses have advanced our knowledge of GA signaling pathways and related genes in model plant species. However, functional genomics analyses of GA signaling pathways in , a perennial herb, have rarely been carried out, despite its well-known economical and medicinal importance. Here, we conducted functional characterization of GA receptors and investigated their physiological roles in the secondary growth of storage roots. We found that the physiological and genetic functions of gibberellin-insensitive dwarf1s (PgGID1s) have been evolutionarily conserved. Additionally, the essential domains and residues in the primary protein structure for interaction with active GAs and DELLA proteins are well-conserved. Overexpression of in completely restored the GA deficient phenotype of the () double mutant. Exogenous GA treatment greatly enhanced the secondary growth of tap roots; however, paclobutrazol (PCZ), a GA biosynthetic inhibitor, reduced root growth in . Transcriptome profiling of roots revealed that GA-induced root secondary growth is closely associated with cell wall biogenesis, the cell cycle, the jasmonic acid (JA) response, and nitrate assimilation, suggesting that a transcriptional network regulate root secondary growth in . These results provide novel insights into the mechanism controlling secondary root growth in .
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http://dx.doi.org/10.3390/ijms22168694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395461PMC
August 2021

Analysis of factors influencing improvement of idiopathic flatfoot.

Medicine (Baltimore) 2021 Aug;100(32):e26894

Department of Orthopedic Surgery, Soon Chun Hyang University Cheonan Hospital, Cheonan, South Korea.

Abstract: Idiopathic flatfoot is common in infants and children, and patients with this condition are frequently referred to pediatric orthopedic clinics. Flatfoot is a physiologic process, and that the arch of the foot elevates spontaneously in most children during the first decade of life. To achieve a consensus as the rate of spontaneous improvement of flatfoot, the present study aimed to estimate the rate of spontaneous improvement of flatfoot and to analyze correlating factors.We reviewed the records of patients examined between May 2013 and May 2019 so as to identify those factors associated with idiopathic flatfoot below 12 years of age. We included patients with who had been followed for >6 months, and those for whom ≥2 (anteroposterior and lateral) weight-bearing bilateral radiographs of the foot had been obtained. The progression rates of the anteroposterior (AP) talo-first metatarsal angle, talonavicular coverage angle, lateral talo-first metatarsal angle, and calcaneal pitch angle were adjusted by multiple factors using a linear mixed model, with sex, body mass index, and Achilles tendon contracture as the fixed effects and age and each subject as the random effects.We found that 4 of the radiographic measurements improved as patients grew older. The AP talo-first metatarsal angle, talonavicular coverage angle, and the lateral talo-first metatarsal angle decreased, while the calcaneal pitch angle increased. The AP talo-first metatarsal angle (P < .001), talonavicular coverage angle (P < .001), and lateral talo-first metatarsal angle (P < .001) improved significantly; however, the calcaneal pitch angle (P = .367) did not show any significant difference. In general, the flatfeet showed an improving trend; after analyzing the factors, no sex difference was observed (P = .117), while body mass index (P < .001) and Achilles tendon contracture (P < .001) showed a negative correlation.The study demonstrated that children's flatfeet spontaneously improved at the age of 12 years. It would be more beneficial if the clinician shows the predicted appearance of the foot at the completion of growth by calculating the radiographic indices and identifying the correlating factors in addition to explaining that flatfoot may gradually improve. This will prevent unnecessary medical expenses and the psychological adverse effects to the children caused by unnecessary treatment.
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http://dx.doi.org/10.1097/MD.0000000000026894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360408PMC
August 2021

Diverting ileostomy itself may not increase the rate of postoperative readmission related to dehydration after low anterior resection.

Ann Surg Treat Res 2021 Aug 29;101(2):111-119. Epub 2021 Jul 29.

Center for Colorectal Cancer, Research Institute and Hospital, National Cancer Center, Goyang, Korea.

Purpose: This study was performed to evaluate the risk of readmission in the first year after low anterior resection (LAR) for patients with rectal cancer and to identify the contributing factors for readmission related to dehydration specifically.

Methods: This was a retrospective analysis of 570 patients who underwent LAR for rectal cancer at National Cancer Center, Republic of Korea. A diverting loop ileostomy was performed in 357 (62.6%) of these patients. Readmission was defined as an unplanned visit to the emergency room or admission to the ward. The reasons for readmission were reviewed and compared between the ileostomy (n = 357) and no-ileostomy (n = 213) groups. The risk factors for readmission and readmission due to dehydration were analyzed using multivariable logistic and Cox proportional hazard model.

Results: Dehydration was the most common cause of readmission in both groups (ileostomy group, 6.7%, and no-ileostomy group, 4.7%, P = 0.323). On multivariable analysis, risk factors for readmission were an estimated intraoperative blood loss of ≥400 mL (odds ratio [OR], 1.757; 95% confidence interval [CI], 1.058-2.918; P = 0.029), and postoperative chemotherapy (OR, 2.914; 95% CI, 1.824-4.653; P < 0.001). On multivariable analysis, postoperative chemotherapy, and not a diverting loop ileostomy, was an independent risk factor for dehydration-related readmission (OR, 5.102; 95% CI, 1.772-14.688; P = 0.003).

Conclusion: The most common cause of readmission after LAR for rectal cancer was dehydration, as reported previously. Postoperative chemotherapy, not the creation of a diverting ileostomy, was identified as the risk factor associated with readmission related to dehydration.
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http://dx.doi.org/10.4174/astr.2021.101.2.111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8331557PMC
August 2021

Clinical validation of optimised RT-LAMP for the diagnosis of SARS-CoV-2 infection.

Sci Rep 2021 08 10;11(1):16193. Epub 2021 Aug 10.

Department of Microbiology, Oxford University NHS Foundation Trust, Oxford, UK.

We have optimised a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SARS-CoV-2 from extracted RNA for clinical application. We improved the stability and reliability of the RT-LAMP assay by the addition of a temperature-dependent switch oligonucleotide to reduce self- or off-target amplification. We then developed freeze-dried master mix for single step RT-LAMP reaction, simplifying the operation for end users and improving long-term storage and transportation. The assay can detect as low as 13 copies of SARS-CoV2 RNA per reaction (25-μL). Cross reactivity with other human coronaviruses was not observed. We have applied the new RT-LAMP assay for testing clinical extracted RNA samples extracted from swabs of 72 patients in the UK and 126 samples from Greece and demonstrated the overall sensitivity of 90.2% (95% CI 83.8-94.7%) and specificity of 92.4% (95% CI 83.2-97.5%). Among 115 positive samples which Ct values were less than 34, the RT-LAMP assay was able to detect 110 of them with 95.6% sensitivity. The specificity was 100% when RNA elution used RNase-free water. The outcome of RT-LAMP can be reported by both colorimetric detection and quantifiable fluorescent reading. Objective measures with a digitized reading data flow would allow for the sharing of results for local or national surveillance.
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http://dx.doi.org/10.1038/s41598-021-95607-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355225PMC
August 2021

A metabolomic approach to elucidate the inhibitory effects of baicalin in pulmonary fibrosis.

Pharm Biol 2021 Dec;59(1):1016-1025

Department of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.

Context: Baicalin, a major flavonoid extracted from Georgi (Lamiaceae), has been shown to exert therapeutic effects on pulmonary fibrosis (PF).

Objective: To use serum metabolomics combined with biochemical and histopathological analyses to clarify anti-PF mechanisms of baicalin on metabolic pathways and the levels of potential biomarkers.

Materials And Methods: Forty male Sprague-Dawley rats were randomly divided into the control, PF model, prednisolone acetate-treated (4.2 mg/kg/day) and baicalin-treated (25 and 100 mg/kg/day) groups. A rat model of PF was established using a tracheal injection of bleomycin, and the respective drugs were administered intragastrically for 4 weeks. Histomorphology of lung tissue was examined after H&E and Masson's trichrome staining. Biochemical indicators including SOD, MDA and HYP were measured. Serum-metabonomic analysis based on UPLC-Q-TOF/MS was used to clarify the changes in potential biomarkers among different groups of PF rats.

Results: Both doses of baicalin effectively alleviated bleomycin-induced pathological changes, and increased the levels of SOD (from 69.48 to 99.50 and 112.30, respectively), reduced the levels of MDA (from 10.91 to 5.0 and 7.53, respectively) and HYP (from 0.63 to 0.41 and 0.49, respectively). Forty-eight potential biomarkers associated with PF were identified. Meanwhile, the metabolic profiles and fluctuating metabolite levels were normalized or partially reversed after baicalin treatment. Furthermore, baicalin was found to improve PF potentially by the regulation of four key biomarkers involving taurine and hypotaurine metabolism, glutathione metabolism, and glycerophospholipid metabolism.

Conclusions: These findings revealed the anti-fibrotic mechanisms of baicalin and it may be considered as an effective therapy for PF.
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http://dx.doi.org/10.1080/13880209.2021.1950192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354164PMC
December 2021

Fuzzy -Means Clustering Algorithm-Based Magnetic Resonance Imaging Image Segmentation for Analyzing the Effect of Edaravone on the Vascular Endothelial Function in Patients with Acute Cerebral Infarction.

Contrast Media Mol Imaging 2021 14;2021:4080305. Epub 2021 Jul 14.

Department of Encephalopathy, Qingdao Fifth People's Hospital, Qingdao 266002, China.

This paper aimed to discuss the denoising ability of magnetic resonance imaging (MRI) images based on fuzzy C-means clustering (FCM) algorithm and the influence of Butylphthalide combined with Edaravone treatment on nerve function and vascular endothelial function in patients with acute cerebral infarction (ACI). Based on FCM algorithm, Markov Random Field (MRF) model algorithm was introduced to obtain a novel algorithm (NFCM), which was compared with FCM and MRF algorithm in terms of misclassification rate (MCR) and difference of Kappa index (KI). 90 patients with ACI diagnosed in hospital from December 2018 to December 2019 were selected as subjects, who were divided into combined treatment group (conventional treatment + Edaravone + Butylphthalide) and Edaravone group (conventional treatment + Edaravone) randomly, each consisting of 45 cases. The National Institutes of Health Stroke Scale (NIHSS) score and endothelial function index level such as plasma nitric oxide (NO), human endothelin-1 (ET-1), and vascular endothelial cell growth factor (VEGF) were compared before and after treatment between the two groups. The results showed that the MCR of NFCM was evidently inferior to FCM and MRF, and the KI was notably higher relative to the other two algorithms. After treatment, the NIHSS score of the combined treatment group was (9.09 ± 1.86) points and that of Edaravone group was (14.97 ± 3.44) points, with evident difference between the two groups ( < 0.05). After treatment, the NO of the combined treatment was (54.63 ± 4.85), and that of Edaravone group was (41.54 ± 5.27), which was considerably different ( < 0.01), and the VEGF and ET-1 of combined treatment group were greatly inferior to Edaravone group ( < 0.01). It was revealed that the novel algorithm based on FCM can obtain more favorable quality and segmentation accuracy of MRI images. Moreover, Butylphthalide combined with Edaravone treatment can effectively improve nerve function, vascular endothelial function, and short-term prognosis in ACI, which was safe and worthy of clinical adoption.
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http://dx.doi.org/10.1155/2021/4080305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295001PMC
July 2021

CtBP modulates Snail-mediated tumor invasion in Drosophila.

Cell Death Discov 2021 Aug 4;7(1):202. Epub 2021 Aug 4.

The First Rehabilitation Hospital of Shanghai, Shanghai Key Laboratory of Signaling and Diseases Research, School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai, 200092, China.

Cancer is one of the most fatal diseases that threaten human health, whereas more than 90% mortality of cancer patients is caused by tumor metastasis, rather than the growth of primary tumors. Thus, how to effectively control or even reverse the migration of tumor cells is of great significance for cancer therapy. CtBP, a transcriptional cofactor displaying high expression in a variety of human cancers, has become one of the main targets for cancer prediction, diagnosis, and treatment. The roles of CtBP in promoting tumorigenesis have been well studied in vitro, mostly based on gain-of-function, while its physiological functions in tumor invasion and the underlying mechanism remain largely elusive. Snail (Sna) is a well-known transcription factor involved in epithelial-to-mesenchymal transition (EMT) and tumor invasion, yet the mechanism that regulates Sna activity has not been fully understood. Using Drosophila as a model organism, we found that depletion of CtBP or snail (sna) suppressed Ras/lgl-triggered tumor growth and invasion, and disrupted cell polarity-induced invasive cell migration. In addition, loss of CtBP inhibits Ras/Sna-induced tumor invasion and Sna-mediated invasive cell migration. Furthermore, both CtBP and Sna are physiologically required for developmental cell migration during thorax closure. Finally, Sna activates the JNK signaling and promotes JNK-dependent cell invasion. Given that CtBP physically interacts with Sna, our data suggest that CtBP and Sna may form a transcriptional complex that regulates JNK-dependent tumor invasion and cell migration in vivo.
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http://dx.doi.org/10.1038/s41420-021-00516-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339073PMC
August 2021

Predicting prognosis in patients with stroke treated with intravenous alteplase through the 24-h trajectory of blood pressure changes.

J Clin Hypertens (Greenwich) 2021 Aug 4. Epub 2021 Aug 4.

Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatic Disease, Beijing, China.

Blood pressure (BP) monitored within 24 h from the beginning of intravenous thrombolysis (IVT) with alteplase, is one of the important factors affecting the prognosis of patients with acute ischemic stroke (AIS). This study aimed to explore longitudinal BP trajectory patterns and determine their association with stroke prognosis after thrombolysis. From November 2018 to September 2019, a total of 391 patients were enrolled consecutively during the study period, and 353 patients were ultimately analyzed. Five systolic (SBP) and four diastolic blood pressure (DBP) trajectory subgroups were identified. The regression analysis showed that when compared with the rapidly moderate stable group, the continuous fluctuation-very high level SBP group (odds ratio [OR]: 2.743, 95% confidence interval [CI]: 1.008-7.467) was associated with early neurological deterioration (END). Both the rapid drop-high level SBP (OR: 0.448, 95% CI: 0.219-0.919) and DBP groups (OR: 0.399, 95% CI: 0.219-0.727) were associated with early neurological improvement (ENI). Moreover, there was a U-shaped correlation between the OR value of SBP trajectory group and favorable outcome (the modified Rankin Scale [mRS] score 0-2) at 3 months: the slow drop-low level SBP group represent a well-established unfavorable outcome risk factor (OR:5.239, 95% CI: 1.271-21.595), and extremely high SBP-the continuous fluctuation-very high level SBP group, are equally associated with elevated unfavorable outcome risk (OR:3.797, 95% CI: 1.486-9.697). The continuous fluctuation-very high level DBP group was statistically significant in mRS (OR: 3.387, CI: 1.185-9.683). The BP trajectory groups show varying clinical features and risk of neurological dysfunction. The findings may help identify potential candidates for clinical BP monitoring, control, and specialized care.
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http://dx.doi.org/10.1111/jch.14331DOI Listing
August 2021

Diamine Functionalization of a Metal-Organic Framework by Exploiting Solvent Polarity for Enhanced CO Adsorption.

ACS Appl Mater Interfaces 2021 Aug 3;13(32):38358-38364. Epub 2021 Aug 3.

Department of Chemistry, Korea University, Seoul 02841, Republic of Korea.

Diamine-appended metal-organic frameworks (MOFs) exhibit exceptional CO adsorption capacities over a wide pressure range because of the strong interaction between basic amine groups and acidic CO. Given that their high CO working capacity is governed by solvent used during amine functionalization, a systematic investigation on solvent effect is essential but not yet demonstrated. Herein, we report a facile one-step solvent exchange route for the diamine functionalization of MOFs with open metal sites, using an efficient method to maximize diamine loading. We employed an MOF, Mg(dobpdc) (dobpdc = 4,4'-dioxido-3,3'-biphenyldicarboxylate), which contains high-density open metal sites. Indirect grafting with -ethylethylenediamine (een) was performed with a minimal amount of methanol (MeOH) via multiple MeOH exchanges and diamine functionalization, resulting in a top-tier CO adsorption capacity of 16.5 wt %. We established the correlation between ,-dimethylformamide (DMF) loading and infrared peaks, which provides a simple method for determining the amount of the remaining DMF in Mg(dobpdc). All interactions among Mg, DMF, diamine, and solvent were analyzed by van der Waals (vdw)-corrected density functional theory (DFT) calculations to elucidate the effect of chemical potential on diamine grafting.
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http://dx.doi.org/10.1021/acsami.1c10659DOI Listing
August 2021

Doubly Modulated Optical Lattice Clock: Interference and Topology.

Phys Rev Lett 2021 Jul;127(3):033601

Key Laboratory of Time and Frequency Primary Standards, National Time Service Center, Chinese Academy of Sciences, Xi'an 710600, China.

The quantum system under periodical modulation is the simplest path to understand the quantum nonequilibrium system because it can be well described by the effective static Floquet Hamiltonian. Under the stroboscopic measurement, the initial phase is usually irrelevant. However, if two uncorrelated parameters are modulated, their relative phase cannot be gauged out so that the physics can be dramatically changed. Here, we simultaneously modulate the frequency of the lattice laser and the Rabi frequency in an optical lattice clock (OLC) system. Thanks to the ultrahigh precision and ultrastability of the OLC, the relative phase could be fine-tuned. As a smoking gun, we observed the interference between two Floquet channels. Finally, by experimentally detecting the eigenenergies, we demonstrate the relation between the effective Floquet Hamiltonian and the one-dimensional topological insulator with a high winding number. Our experiment not only provides a direction for detecting the phase effect but also paves a way in simulating the quantum topological phase in the OLC platform.
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http://dx.doi.org/10.1103/PhysRevLett.127.033601DOI Listing
July 2021

Metabolomics reveals the renoprotective effect of -butanol extract and amygdalin extract from in rats with renal fibrosis.

Artif Cells Nanomed Biotechnol 2021 Dec;49(1):556-564

Department of Pharmacy, Baotou Medical College, Baotou, China.

Renal fibrosis (RF) is a pathological process of progression from chronic kidney disease to end-stage renal disease. is a traditional Chinese medicine, and our previous studies demonstrated that the n-butanol extract (BUT) and amygdalin extract (AMY) from its seeds can prevent RF. However, the underlying mechanism remains unclear. The present study investigated the exact mechanism of the protective effect of on RF. A renal fibrosis rat model was induced with unilateral ureteral obstruction. Biochemical indicators were measured and combined with histopathology of renal tissue to evaluate the anti-RF effects. A serum metabonomic method was used to clarify the changes in the metabolic profile. The tubulointerstitial damage and fibrosis were significantly improved and metabolic perturbations were restored after treatment with BUT and AMY. Thirty-eight metabolites associated with RF progression and related to the regulation of arginine and proline metabolism, nicotinate and nicotinamide metabolism, and histidine metabolism were identified. They were restored to levels similar to those in controls after treatment. Moreover, no significant differences in efficacy were observed between the BUT and AMY groups. This study reveals and compares the potential mechanisms of the renoprotective effects after treatment with BUT and AMY from a metabolomic perspective.
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http://dx.doi.org/10.1080/21691401.2021.1952212DOI Listing
December 2021

The Conservation of Chloroplast Genome Structure and Improved Resolution of Infrafamilial Relationships of Crassulaceae.

Front Plant Sci 2021 1;12:631884. Epub 2021 Jul 1.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.

Crassulaceae are the largest family in the angiosperm order Saxifragales. Species of this family are characterized by succulent leaves and a unique photosynthetic pathway known as Crassulacean acid metabolism (CAM). Although the inter- and intrageneric relationships have been extensively studied over the last few decades, the infrafamilial relationships of Crassulaceae remain partially obscured. Here, we report nine newly sequenced chloroplast genomes, which comprise several key lineages of Crassulaceae. Our comparative analyses and positive selection analyses of Crassulaceae species indicate that the overall gene organization and function of the chloroplast genome are highly conserved across the family. No positively selected gene was statistically supported in Crassulaceae lineage using likelihood ratio test (LRT) based on branch-site models. Among the three subfamilies of Crassulaceae, our phylogenetic analyses of chloroplast protein-coding genes support Crassuloideae as sister to Kalanchoideae plus Sempervivoideae. Furthermore, within Sempervivoideae, our analyses unambiguously resolved five clades that are successively sister lineages, i.e., Telephium clade, Sempervivum clade, Aeonium clade, Leucosedum clade, and Acre clade. Overall, this study enhances our understanding of the infrafamilial relationships and the conservation of chloroplast genomes within Crassulaceae.
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http://dx.doi.org/10.3389/fpls.2021.631884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281817PMC
July 2021

MiR-222-3p induced by hepatitis B virus promotes the proliferation and inhibits apoptosis in hepatocellular carcinoma by upregulating THBS1.

Hum Cell 2021 Jul 17. Epub 2021 Jul 17.

Department of Hepatobiliary Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinnan, 250022, Shandong, People's Republic of China.

This study aimed to explore the role of miR-222-3p in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). MiR-222-3p expression in tumor tissues of HBV (+) or HBV (-) HCC patients and corresponding cell lines was detected by quantitative reverse transcription PCR (qRT-PCR). Cell proliferation was assessed by cell counting kit-8 (CCK-8) and colony formation assays. Cell apoptosis was evaluated by flow cytometry. The potential targets of miR-222-3p were predicted by Targetscan, and the binding relationship between miR-222-3p and thrombospondin-1 (THBS1) was determined by luciferase reporter assay and RNA immunoprecipitation (RIP) assay. MiR-222-3p was significantly upregulated in HCC tissues and cell lines and further elevated by HBV infection. MiR-222-3p downregulation effectively inhibited the proliferation and induced the apoptosis of HBV (-) HepG2 cells, HBV (+) HepG2.2.15 cells, Huh7-V cells, and Huh7-HBV cells. In addition, miR-222-3p overexpression enhanced the proliferation of these cell lines but exhibited no obvious effect on their apoptosis. Mechanistically, miR-222-3p was directly bound to the 3'-UTR of THBS1 and acted as its competing endogenous RNA (ceRNA). Interestingly, THBS1 silencing attenuated the inhibitory effect of miR-222-3p downregulation on the proliferation of these cell lines in vitro. Our results revealed that HBV infection further increased miR-222-3p expression and promoted HCC progression via miR-222-3p-mediated THBS1 downregulation. Our findings suggest that miR-222-3p might be a potential diagnostic and therapeutic target for HCC and HBV-related HCC.
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http://dx.doi.org/10.1007/s13577-021-00577-1DOI Listing
July 2021

Intratumor Heterogeneity of MIF Expression Correlates With Extramedullary Involvement of Multiple Myeloma.

Front Oncol 2021 29;11:694331. Epub 2021 Jun 29.

Department of Hematology, Institute of Hematology, West China Hospital, Sichuan University, Chengdu, China.

Macrophage migration inhibitory factor (MIF) has been shown to promote disease progression in many malignancies, including multiple myeloma (MM). We previously reported that MIF regulates MM bone marrow homing and knockdown of MIF favors the extramedullary myeloma formation in mice. Here, based on MIF immunostaining of myeloma cells in paired intramedullary and extramedullary biopsies from 17 patients, we found lower MIF intensity in extramedullary MM (EMM) versus intramedullary MM (IMM). Flow cytometry and histology analysis in xenograft models showed a portion of inoculated human MM cells lost their MIF expression (MIF) . Of note, IMM had dominantly MIF cells, while EMM showed a significantly increased ratio of MIF cells. Furthermore, we harvested the extramedullary human MM cells from a mouse and generated single-cell transcriptomic data. The developmental trajectories of MM cells from the MIF to MIF state were indicated. The MIF cells featured higher proliferation. The MIF ones were more quiescent and harbored abundant ribosomal protein genes. Our findings identified differential regulation of MIF expression in MM and suggested a potential pathogenic role of MIF in the extramedullary spread of disease.
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http://dx.doi.org/10.3389/fonc.2021.694331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276700PMC
June 2021

Mitigation of global warming potential and greenhouse gas intensity in arable soil with green manure as source of nitrogen.

Environ Pollut 2021 Jul 6;288:117724. Epub 2021 Jul 6.

Department of Life Science and Environmental Biochemistry, Pusan National University, Miryang, 50463, Republic of Korea; Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Republic of Korea. Electronic address:

This study was conducted to determine the effect of different green manure treatments on net GWP and GHGI in upland soil. Barley (B), hairy vetch (HV), and a barley/hairy vetch mixture (BHV) were sown on an upland soil on November 4, 2017 and October 24, 2018. The aboveground biomass of these green manures was incorporated into soil on June 1, 2018 and May 8, 2019. In addition, a fallow treatment (F) was installed as the control. Maize was transplanted as the subsequent crop after incorporation of green manures. Green manuring significantly affected CO and NO emission, but not CH. Average cumulative soil respiration across years with HV and BHV were 37.0 Mg CO ha yr and 35.8 Mg CO ha yr, respectively and significantly higher than those with under F and B (32.7 Mg CO ha yr and 33.0 Mg CO ha yr, respectively). Cumulative NO emissions across years with F and HV were 6.29 kg NO ha yr and 5.44 kg NO ha yr, respectively and significantly higher than those with B and BHV (4.26 kg NO ha yr and 4.42 kg NO ha yr, respectively). The net ecosystem carbon budget for HV (-0.5 Mg C ha yr) was the greatest among the treatments (F; -1.61 Mg C ha yr, B; -3.98 Mg C ha yr, and BHV; -0.91 Mg C ha yr) because of its high biomass yields and the yield of maize after incorporation of HV. There was no significant difference of GHGI among F, HV, and BHV. Incorporation of HV or BHV could reduce net CO emissions per unit of maize grain production as well as F.
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http://dx.doi.org/10.1016/j.envpol.2021.117724DOI Listing
July 2021

Nanostructured TiO Sensitized with MoS Nanoflowers for Enhanced Photodegradation Efficiency toward Methyl Orange.

ACS Omega 2021 Jul 23;6(26):17071-17085. Epub 2021 Jun 23.

Nanomaterials Research Laboratory, Department of Chemistry, Shivaji University, Kolhapur, Maharashtra 416004, India.

Nanostructured titanium dioxide (TiO) has a potential platform for the removal of organic contaminants, but it has some limitations. To overcome these limitations, we devised a promising strategy in the present work, the heterostructures of TiO sensitized by molybdenum disulfide (MoS) nanoflowers synthesized by the mechanochemical route and utilized as an efficient photocatalyst for methyl orange (MO) degradation. The surface of TiO sensitized by MoS was comprehensively characterized by X-ray diffraction (XRD), Raman spectroscopy, Fourier transform-infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), energy dispersive spectroscopy (EDS), UV-vis diffuse reflectance spectroscopy (UV-vis DRS), photoluminescence spectroscopy (PL), Brunauer-Emmett-Teller (BET) surface area, and thermogravimetric analysis (TGA). From XRD results, the optimized MoS-TiO (5.0 wt %) nanocomposite showcases the lowest crystallite size of 14.79 nm than pristine TiO (20 nm). The FT-IR and XPS analyses of the MoS-TiO nanocomposite exhibit the strong interaction between MoS and TiO. The photocatalytic results show that sensitization of TiO by MoS drastically enhanced the photocatalytic activity of pristine TiO. According to the obtained results, the optimal amount of MoS loading was assumed to be 5.0 wt %, which exhibited a 21% increment of MO photodegradation efficiency compared to pristine TiO under UV-vis light. The outline of the overall study describes the superior photocatalytic performance of 5.0 wt % MoS-TiO nanocomposite which is ascribed to the delayed recombination by efficient charge transfer, high surface area, and elevated surface oxygen vacancies. The context of the obtained results designates that the sensitization of TiO with MoS is a very efficient nanomaterial for photocatalytic applications.
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http://dx.doi.org/10.1021/acsomega.1c02194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264933PMC
July 2021

Prognostic significance of preoperative systemic inflammatory biomarkers in patients with hepatocellular carcinoma after microwave ablation and establishment of a nomogram.

Sci Rep 2021 Jul 5;11(1):13814. Epub 2021 Jul 5.

Department of Hepatobiliary Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, No. 9677, Jingshi Road, Lixia District, Jinan, 500212, Shandong, China.

The study aimed to evaluate the prognostic significance of preoperative systemic inflammatory biomarkers including albumin to globulin ratio (AGR), neutrophil to lymphocyte ratio (NLR), lymphocyte to monocyte ratio (LMR), and platelet to lymphocyte ratio (PLR) and establish a nomogram in hepatocellular carcinoma (HCC) patients after microwave ablation (MWA). 192 HCC patients receiving MWA as initial therapy from the first ward of hepatobiliary surgery were classified as training cohort. Whereas, 84 patients from the second of hepatobiliary surgery were classified as validation cohort. Kaplan-Meier (KM) method and univariate analyses showed that AGR, NLR, LMR, and PLR were significantly associated with OS in the training cohort. Multivariate analysis including clinicopathologic features screened out independent predictors including ascites, tumor size, cancer embolus, AGR, and PLR. Based on those variables, a nomogram for predicting OS was established. The C-index was 0.794 in the training cohort and 0.772 in the validation cohort. Calibration plots identified the nomogram performed well with an ideal model. Compared with Barcelona Clinic Liver Cancer (BCLC) staging system and simple tumor size, the nomogram showed better predictive ability. Besides, the nomogram discovered the highest diagnostic accuracy in predicting postoperative clinical outcome than the combination of the present models with tumor size. In conclusion, the constructed nomogram could accurately predict individualized survival probability and might support clinician in individual treatment optimization and clinical decision-making.
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http://dx.doi.org/10.1038/s41598-021-93289-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257620PMC
July 2021

Whole-genome, transcriptome, and methylome analyses provide insights into the evolution of platycoside biosynthesis in Platycodon grandiflorus, a medicinal plant.

Hortic Res 2020 Jul 1;7(1):112. Epub 2020 Jul 1.

Genomics Division, National Institute of Agricultural Sciences (NAS), Jeonju, 54874, Korea.

Triterpenoid saponins (TSs) are common plant defense phytochemicals with potential pharmaceutical properties. Platycodon grandiflorus (Campanulaceae) has been traditionally used to treat bronchitis and asthma in East Asia. The oleanane-type TSs, platycosides, are a major component of the P. grandiflorus root extract. Recent studies show that platycosides exhibit anti-inflammatory, antiobesity, anticancer, antiviral, and antiallergy properties. However, the evolutionary history of platycoside biosynthesis genes remains unknown. In this study, we sequenced the genome of P. grandiflorus and investigated the genes involved in platycoside biosynthesis. The draft genome of P. grandiflorus is 680.1 Mb long and contains 40,017 protein-coding genes. Genomic analysis revealed that the CYP716 family genes play a major role in platycoside oxidation. The CYP716 gene family of P. grandiflorus was much larger than that of other Asterid species. Orthologous gene annotation also revealed the expansion of β-amyrin synthases (bASs) in P. grandiflorus, which was confirmed by tissue-specific gene expression. In these expanded gene families, we identified key genes showing preferential expression in roots and association with platycoside biosynthesis. In addition, whole-genome bisulfite sequencing showed that CYP716 and bAS genes are hypomethylated in P. grandiflorus, suggesting that epigenetic modification of these two gene families affects platycoside biosynthesis. Thus whole-genome, transcriptome, and methylome data of P. grandiflorus provide novel insights into the regulation of platycoside biosynthesis by CYP716 and bAS gene families.
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http://dx.doi.org/10.1038/s41438-020-0329-xDOI Listing
July 2020

Clinicopathological features of desmoplastic small round cell tumors: clinical series and literature review.

World J Surg Oncol 2021 Jun 30;19(1):193. Epub 2021 Jun 30.

Department of Pathology, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, People's Republic of China.

Background And Purpose: Desmoplastic small round cell tumor (DSRCT) is a highly malignant sarcoma that occurs in the abdominopelvic cavities of adolescents. The accurate diagnosis of DSRCT is challenging owing to limited literatures. Our study aimed to investigate the relationship between clinicopathological features and prognosis in patients with DSRCTs.

Methods: Data of 8 patients with DSRCT originating from the abdominal cavity were retrospectively reviewed. The clinical manifestations, pathological characteristics, treatment approaches, and prognosis were analyzed. The histopathological (identified using hematoxylin-eosin staining), immunohistochemical, and molecular diagnostic (using fluorescence in situ hybridization) features were also reviewed.

Results: All patients were male aged between 24 and 45 years (median age, 30 years). The main clinical symptoms included abdominal distension, abdominal pain, and constipation. Seven of the 8 patients developed metastases to either distant organs or lymph nodes. Multiple gray nodules with diameters of 1-10 cm and poorly defined boundaries were scattered throughout the omentum and mesentery. Histopathological examination demonstrated well-defined nests composed of small round blue cells separated by markedly desmoplastic stroma. Immunohistochemical analysis revealed positive expressions of desmin, vimentin and C-terminal of Wilm's tumor suppressor (WT-1). The Ewing sarcoma breakpoint region 1 gene fused with WT1 (EWSR1-WT1) gene fusion was detected in all patients. Cytoreductive surgery (CRS) was performed in 6 patients. Follow-up period ranged from 7.5 to 28.5 months with a median of 17.2 months. Three patients died during follow-up.

Conclusion: DSRCT is highly aggressive and presents distinctive morphological features. CRS is the essential therapy for DSRCT. A test for the combined expression of desmin, cytokeratins, and C-terminal of WT-1, as well as the analysis of morphologic features, might be helpful during DSRCT diagnosis, and the EWSR1-WT1 gene fusion is the gold standard for definitive diagnosis. Our work will provide new insights into the diagnosis and treatment of DSRCTs.
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http://dx.doi.org/10.1186/s12957-021-02310-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247134PMC
June 2021

Prognostic significance of CEA, Ki67 and p53 in pseudomyxoma peritonei of appendiceal origin.

J Int Med Res 2021 Jun;49(6):3000605211022297

Department of Pathology, 117968Beijing Shijitan Hospital, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Objective: To determine the levels of carcinoembryonic antigen (CEA), proliferating nuclear antigen Ki67 and p53 in pseudomyxoma peritonei (PMP) of appendiceal origin and to correlate the levels with clinicopathological characteristics and overall survival.

Methods: This retrospective study collected data on clinicopathological features and immunohistochemical staining of CEA, Ki67 and p53 in patients with PMP of appendiceal origin. Overall survival was evaluated using Kaplan-Meier plots. Median survival time was estimated by Log-rank tests. Potential prognostic factors were evaluated by Cox proportional hazards regression models.

Results: A total of 141 patients with PMP of appendiceal origin were enrolled in the study with a median age of 54 years. Of these, 93 (66.0%) were diagnosed with low-grade mucinous carcinoma, 43 (30.5%) with high-grade mucinous carcinoma and five (3.5%) with high-grade with signet ring cells. CEA exhibited ubiquitous immunopositivity in most cases and was not associated with overall survival. Ki67 labelling index (LI) and p53 status were related to histological grade and overall survival. The main pathological indicators affecting survival included histological grade, lymph node involvement, angiolymphatic invasion, Ki67 LI and p53.

Conclusion: Combined analysis of high Ki67 LI and aberrant p53 may provide the basis for evaluating the biological behaviour of PMP and predicting clinical outcome.
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http://dx.doi.org/10.1177/03000605211022297DOI Listing
June 2021

Synthesis and Crystal Structures of New Strontium Complexes with Aminoalkoxy and β-Diketonato Ligands.

ACS Omega 2021 Jun 7;6(24):15948-15956. Epub 2021 Jun 7.

Thin Film Materials Research Center, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, Republic of Korea.

New heteroleptic strontium complexes were synthesized using substitution reaction of bis(trimethylsilyl)amide of Sr(btsa)·2DME with aminoalkoxide and β-diketonate ligands. The complexes [Sr(bdmp)(btsa)]·2THF (), [Sr(bdeamp)(btsa)] (), [Sr(dadamb)(btsa)] (), [Sr(bdmp)(hfac)] (), [Sr(bdeamp)(hfac)] (), [Sr(dadamb)(hfac)] (), and [Sr(dadamb)(tmhd)] () were prepared and characterized by means of various analysis techniques such as Fourier transform infrared, NMR, thermogravimetric analysis, and elemental analysis. Complexes were further structurally confirmed by single-crystal X-ray crystallography, and they displayed dimeric structures in which strontium atoms were connected by alkoxide oxygen atoms of the μ type. Compound has a trigonal prismatic structure, whereas and have a distorted square pyramidal structure. In complexes , trimeric structures were obtained with strontium atoms connected by μ-O bonds of alkoxide oxygen atoms and μ-O bonds of alkoxide and β-diketonate oxygen atoms. The crystal structures of , , and showed distorted capped octahedral geometry, while (middle Sr atom) displayed a distorted trigonal prism geometry. Complexes displayed ∼70% mass loss in the temperature range from 25 to 315 °C.
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http://dx.doi.org/10.1021/acsomega.1c01624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223417PMC
June 2021

Identifying novel genetic variants for brain amyloid deposition: a genome-wide association study in the Korean population.

Alzheimers Res Ther 2021 06 21;13(1):117. Epub 2021 Jun 21.

Department of Digital Health, SAIHST, Sungkyunkwan University, Samsung Medical Center, 81 Irwon-ro, Gangnam-gu, Seoul, 06351, Republic of Korea.

Background: Genome-wide association studies (GWAS) have identified a number of genetic variants for Alzheimer's disease (AD). However, most GWAS were conducted in individuals of European ancestry, and non-European populations are still underrepresented in genetic discovery efforts. Here, we performed GWAS to identify single nucleotide polymorphisms (SNPs) associated with amyloid β (Aβ) positivity using a large sample of Korean population.

Methods: One thousand four hundred seventy-four participants of Korean ancestry were recruited from multicenters in South Korea. Discovery dataset consisted of 1190 participants (383 with cognitively unimpaired [CU], 330 with amnestic mild cognitive impairment [aMCI], and 477 with AD dementia [ADD]) and replication dataset consisted of 284 participants (46 with CU, 167 with aMCI, and 71 with ADD). GWAS was conducted to identify SNPs associated with Aβ positivity (measured by amyloid positron emission tomography). Aβ prediction models were developed using the identified SNPs. Furthermore, bioinformatics analysis was conducted for the identified SNPs.

Results: In addition to APOE, we identified nine SNPs on chromosome 7, which were associated with a decreased risk of Aβ positivity at a genome-wide suggestive level. Of these nine SNPs, four novel SNPs (rs73375428, rs2903923, rs3828947, and rs11983537) were associated with a decreased risk of Aβ positivity (p < 0.05) in the replication dataset. In a meta-analysis, two SNPs (rs7337542 and rs2903923) reached a genome-wide significant level (p < 5.0 × 10). Prediction performance for Aβ positivity increased when rs73375428 were incorporated (area under curve = 0.75; 95% CI = 0.74-0.76) in addition to clinical factors and APOE genotype. Cis-eQTL analysis demonstrated that the rs73375428 was associated with decreased expression levels of FGL2 in the brain.

Conclusion: The novel genetic variants associated with FGL2 decreased risk of Aβ positivity in the Korean population. This finding may provide a candidate therapeutic target for AD, highlighting the importance of genetic studies in diverse populations.
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http://dx.doi.org/10.1186/s13195-021-00854-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215820PMC
June 2021
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