Holger Spiegel

Holger Spiegel

UNVERIFIED PROFILE

Are you Holger Spiegel?   Register this Author

Register author
Holger Spiegel

Holger Spiegel

Publications by authors named "Holger Spiegel"

Are you Holger Spiegel?   Register this Author

36Publications

802Reads

35Profile Views

Ready-to-Use Stocks of Agrobacterium tumefaciens Can Simplify Process Development for the Production of Recombinant Proteins by Transient Expression in Plants.

Biotechnol J 2019 Oct 18;14(10):e1900113. Epub 2019 Jul 18.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074, Aachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/biot.201900113DOI Listing
October 2019

Simplified Tracking of a Soy Allergen in Processed Food Using a Monoclonal Antibody-Based Sandwich ELISA Targeting the Soybean 2S Albumin Gly m 8.

J Agric Food Chem 2019 Aug 29;67(31):8660-8667. Epub 2019 Jul 29.

Fraunhofer Institute for Cell Therapy and Immunology IZI , Perlickstraße 1 , 04103 Leipzig , Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.9b02717DOI Listing
August 2019

Critical Analysis of the Commercial Potential of Plants for the Production of Recombinant Proteins.

Front Plant Sci 2019 11;10:720. Epub 2019 Jun 11.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2019.00720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579924PMC
June 2019

Aspergillus-specific antibodies - Targets and applications.

Biotechnol Adv 2018 Jul - Aug;36(4):1167-1184. Epub 2018 Mar 30.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biotechadv.2018.03.016DOI Listing
February 2019

Downstream processing of a plant-derived malaria transmission-blocking vaccine candidate.

Protein Expr Purif 2018 12 27;152:122-130. Epub 2018 Jul 27.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany; Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany. Electronic address:

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S10465928173059
Publisher Site
http://dx.doi.org/10.1016/j.pep.2018.07.012DOI Listing
December 2018

Proteomic analysis of CHO cell lines producing high and low quantities of a recombinant antibody before and after selection with methotrexate.

J Biotechnol 2018 Jan 12;265:65-69. Epub 2017 Nov 12.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2017.11.008DOI Listing
January 2018

A Plant-Based Transient Expression System for the Rapid Production of Malaria Vaccine Candidates.

Methods Mol Biol 2016 ;1404:597-619

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074, Aachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-3389-1_39DOI Listing
December 2016

Development of a Competitive Cystatin C-Specific Bioassay Suitable for Repetitive Measurements.

PLoS One 2016 22;11(1):e0147177. Epub 2016 Jan 22.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany.

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0147177PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4723070PMC
July 2016

Optimized Blanching Reduces the Host Cell Protein Content and Substantially Enhances the Recovery and Stability of Two Plant-Derived Malaria Vaccine Candidates.

Front Plant Sci 2016 17;7:159. Epub 2016 Feb 17.

Integrated Production Platforms, Fraunhofer-Institute for Molecular Biology and Applied Ecology IMEAachen, Germany; Institute for Molecular Biotechnology, RWTH Aachen UniversityAachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2016.00159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756251PMC
February 2016

Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development.

Front Plant Sci 2015 23;6:1169. Epub 2015 Dec 23.

Fraunhofer Institute for Molecular Biology and Applied Ecology IMEAachen, Germany; Institute for Molecular Biotechnology, RWTH Aachen UniversityAachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2015.01169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4688378PMC
January 2016

Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50.

BMC Biotechnol 2015 Dec 1;15:108. Epub 2015 Dec 1.

Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Forckenbeckstrasse 6, 52074, Aachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12896-015-0225-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4665938PMC
December 2015

From gene to harvest: insights into upstream process development for the GMP production of a monoclonal antibody in transgenic tobacco plants.

Plant Biotechnol J 2015 Oct 27;13(8):1094-105. Epub 2015 Jul 27.

Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.12438DOI Listing
October 2015

Heat-precipitation allows the efficient purification of a functional plant-derived malaria transmission-blocking vaccine candidate fusion protein.

Biotechnol Bioeng 2015 Jul 13;112(7):1297-305. Epub 2015 Mar 13.

Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Forckenbeckstrasse 6, 52074, Aachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.25548DOI Listing
July 2015

A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates.

Biotechnol Bioeng 2015 May 16;112(5):867-78. Epub 2015 Jan 16.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, Aachen, 52074, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.25502DOI Listing
May 2015

An immunohistochemical assay on human tissue using a human primary antibody.

J Immunoassay Immunochem 2014 ;35(3):322-34

a Fraunhofer Institute for Molecular Biology and Applied Ecology IME , Aachen , Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/15321819.2013.864974DOI Listing
November 2014

Protective oral vaccination against infectious bursal disease virus using the major viral antigenic protein VP2 produced in Pichia pastoris.

PLoS One 2013 20;8(12):e83210. Epub 2013 Dec 20.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany ; Institute for Phytopathology and Applied Zoology, Phytopathology Department, Justus-Liebig University Giessen, Giessen, Germany.

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0083210PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869785PMC
October 2014

Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system.

BMC Biotechnol 2014 May 3;14:37. Epub 2014 May 3.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstr, 6, 52074 Aachen, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/1472-6750-14-37DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101825PMC
May 2014

Characterization of the single-chain Fv-Fc antibody MBP10 produced in Arabidopsis alg3 mutant seeds.

Transgenic Res 2011 Oct 25;20(5):1033-42. Epub 2010 Dec 25.

Business Unit Bioscience, Plant Research International BV, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6700 AA Wageningen, The Netherlands.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11248-010-9475-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3174376PMC
October 2011

Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit.

J Immunol Methods 2008 Dec 9;339(2):115-23. Epub 2008 Sep 9.

Royal College of Surgeons, Department of Surgery, Connolly Hospital, Blanchardstown, D15, Ireland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jim.2008.08.005DOI Listing
December 2008