Publications by authors named "Ho Yoo"

23 Publications

  • Page 1 of 1

Improved tumor-suppressive effect of OZ-001 combined with cisplatin mediated by mTOR/p70S6K and STAT3 inactivation in A549 human lung cancer cells.

Biomed Pharmacother 2021 Jul 28;142:111961. Epub 2021 Jul 28.

Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, 26, Kyungheedae-ro, Seoul 02447, Republic of Korea; Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Seoul 02447, Republic of Korea. Electronic address:

We previously reported the anticancer activity of 4-(4-fluorobenzylcarbamoylmethyl)-3-(4-cyclohexylphenyl)-2-[3-(N,N-dimethylureido)-N'-methylpropylamino]-3,4-dihydroquinazoline (OZ-001), a T-type calcium channel (TTCC) blocker, against non-small cell lung cancer (NSCLC) in vitro and in vivo. Here, we evaluated the synergistic effect of OZ-001 and cisplatin on A549 human lung cancer cells and A549 xenograft mice. Our study demonstrated that treatment with OZ-001 and cisplatin sensitized A549 cells to cisplatin and significantly inhibited cell growth, increased the number of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells, and induced poly (ADP-ribose) polymerase (PARP) cleavage in A549 cells and an A549 xenograft tumor mouse model. Moreover, our findings showed that mechanistic target of rapamycin (mTOR), ribosomal protein S6 kinase (p70S6K), and signal transducer and activator of transcription (STAT3) inactivation was required for apoptosis induced by the combination of OZ-001 and cisplatin in in vitro and in vivo experiments. Our results suggest that combined treatment with OZ-001 and cisplatin could potentiate antiproliferative effects via suppression of the mTOR/p70S6K and STAT3 pathways and may be considered a potential therapeutic agent for NSCLC.
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http://dx.doi.org/10.1016/j.biopha.2021.111961DOI Listing
July 2021

Luteolin Induces Apoptosis and Autophagy in HCT116 Colon Cancer Cells via p53-Dependent Pathway.

Nutr Cancer 2021 Mar 24:1-10. Epub 2021 Mar 24.

Department of Food and Nutrition, Seoul National University, Seoul, Korea.

Although a dietary phytochemical luteolin has been shown to regulate various anticancer mechanisms, a role of luteolin in autophagy regulation is mostly unidentified. Here, we investigated whether luteolin exhibits its anticancer effects by induction of apoptosis and autophagy in a p53-dependent manner in colon cancer cells. Cell viability was determined using trypan blue exclusion test. The expressions of proteins and mRNAs were measured by immunoblotting and reverse transcription polymerase chain reaction, respectively. Luteolin at 10 - 20 μM induced cytotoxicity in p53 wild-type HCT116 colon cancer cells but not in p53 mutant HT-29 cells and normal colon cells. Luteolin exhibited its anticancer effect by increasing p53 phosphorylation and p53 target gene expression, leading to apoptosis and cell cycle arrest in HCT116 cells. We identified that luteolin can induce autophagy in p53 wild-type cells but not in p53 mutant cells, suggesting that luteolin-induced autophagy is p53-dependent; however, chloroquine-mediated inhibition of autophagy did not alter cytotoxicity and apoptosis of cells treated with luteolin. In conclusion, the present data showed that luteolin inhibits the growth of HCT116 colon cancer cells through p53-dependent regulation of apoptosis and cell cycle arrest regardless of the induction of autophagy.
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http://dx.doi.org/10.1080/01635581.2021.1903947DOI Listing
March 2021

Design, synthesis, and biological evaluation of novel imidazole derivatives possessing terminal sulphonamides as potential BRAFinhibitors.

Bioorg Chem 2021 01 24;106:104508. Epub 2020 Nov 24.

Center for Biomaterials, Korea Institute of Science & Technology (KIST School), Seongbuk-gu, Seoul 02792, Republic of Korea; University of Science & Technology (UST), Yuseong-gu, Daejeon 34113, Republic of Korea. Electronic address:

BRAF mutation has been detected in various malignant tumours. Developing of potent BRAF inhibitors is considered a leading step in the way to cure different cancer types. In the current work, a series of 38 4-(1H-imidazol-5-yl)pyridin-2-amine derivatives was designed and synthesized using Dabrafenib as a lead compound for structural-guided optimization. The target compounds were evaluated as potential anticancer agents against NCI 60 human cancer cell lines. In 5-dose testing mode, two compounds 14h and 16e were tested to determine their IC values over each of the 60 cell lines. The selected candidates exhibited promising activity with mean IC values of 2.4 µM and 3.6 µM, respectively. Melanoma cancer cell lines exhibited the highest sensitivity after the treatment with the tested compounds 14h and 16e. The mean IC values of compounds 14h and 16e against Melanoma cancer cell lines are 1.8 µM and 1.88 µM, respectively. In addition, BRAF kinase inhibitory activity was determined for each derivative. Compounds 15i, 15j, 16a, and 16d were the most potent inhibitors against BRAF with IC 76 nM, 32 nM, 35 nM, and 68 nM. The newly developed compounds represent a therapeutically promising approach for the treating various cancer types.
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http://dx.doi.org/10.1016/j.bioorg.2020.104508DOI Listing
January 2021

Structural optimization of imidazothiazole derivatives affords a new promising series as B-Raf V600E inhibitors; synthesis, in vitro assay and in silico screening.

Bioorg Chem 2020 07 22;100:103967. Epub 2020 May 22.

Center for Biomaterials, Korea Institute of Science & Technology (KIST School), Seoul, Seongbuk-gu 02792, Republic of Korea; University of Science & Technology (UST), Daejeon, Yuseong-gu 34113, Republic of Korea. Electronic address:

BRAF mutation is commonly known in a number of human cancer types. It is counted as a potential component in treating cancer. In this study, based on structural optimization of previously reported inhibitors (3-fluro substituted derivatives of imidazo[2,1-b]thiazole-based scaffold), we designed and synthesized sixteen new imidazo[2,1-b]thiazole derivatives with m-nitrophenyl group at position 6. The electron withdrawing properties was reserved while the polarity was modified compared to previously synthesized compounds (-F). Furthermore, the new substituted group (-NO) provided an additional H-bond acceptor(s) which may bind with the target enzyme through additional interaction(s). In vitro cytotoxicity evaluation was performed against human cancer cell line (A375). In addition, in vitro enzyme assay was performed against mutated B-Raf (B-Raf V600E). Compounds 13a, 13g and 13f showed highest activity on mutated B-Raf with IC 0.021, 0.035 and 0.020 µM. All target compounds were tested for in vitro cytotoxicity against NCI 60 cell lines. Compounds 13a and 13g were selected for 5 doses test mode. Moreover, in silico molecular simulation was explored in order to explore the possible interactions between the designed compounds and the B-Raf V600E active site.
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http://dx.doi.org/10.1016/j.bioorg.2020.103967DOI Listing
July 2020

Drug-Induced Parkinsonism Manifesting as Gait Freezing in a Patient With Traumatic Brain Injury: A Case Report.

Clin Neuropharmacol 2019 May/Jun;42(3):94-96

Department and Research Institute of Rehabilitation Medicine, College of Medicine, Yonsei University, Seoul, Republic of Korea.

Background: Among the neuropsychiatric complications commonly induced by traumatic brain injury (TBI), behavioral disorders, such as agitation and aggression, can hinder neurological recovery and deteriorate rehabilitation outcomes. Pharmacological treatment for behavioral disorders might be beneficial but could lead to drug-induced parkinsonism. We report a case of a patient with drug-induced parkinsonism manifested as freezing of gait after TBI, which improved with the cessation of the offending drugs and comprehensive rehabilitation.

Case Presentation: A 35-year-old male patient left with a TBI after a car accident was referred to our hospital. He had been on many neuropsychiatric medications, including atypical antipsychotics, for his agitated behaviors. He could walk independently but showed freezing of gait at the initiation of his gait, when turning to the side, when reaching his destination, and passing through narrow corridors. Under the impression of drug-induced parkinsonism, we gradually tapered the patient off his neuropsychiatric medications. He also underwent comprehensive rehabilitation, including gait training under visual and auditory cues and balance training. Five weeks after admission to the hospital, the patient's freezing of gait improved, with disappearance of his hesitation at gait initiation and a decreased freezing duration while turning around.

Conclusions: This is a rare report of drug-induced parkinsonism manifested as freezing of gait, which showed improvement after discontinuation of the causative drugs and subsequent rehabilitation.
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http://dx.doi.org/10.1097/WNF.0000000000000333DOI Listing
November 2019

Cognitive improvement effects of electro-acupuncture for the treatment of MCI compared with Western medications: a systematic review and Meta-analysis.

BMC Complement Altern Med 2019 Jan 8;19(1):13. Epub 2019 Jan 8.

Department of Oriental Neuropsychiatry, College of Korean Medicine, College of Oriental Medicine, Daejeon University, Yongun-dong, Dong-gu, Daejeon, South Korea.

Background: Almost half of mild cognitive impairment (MCI) patients progress to dementia, which is associated with decreased quality of life and obstacles to independent living. Relevant management is expected to prevent MCI patients from progressing to dementia. In recent years, electroacupuncture (EA) has been used to treat various kinds of neurological disorders including MCI. This study evaluates the use of EA for MCI patients to increase cognitive function through a comparison with Western medications.

Methods: Randomized controlled trials (RCT) or systematical reviews (SR) of EA versus Western medications for MCI were searched using the following 10 databases: Pubmed, Cochrane Library, CINAHL, EMBASE, China National Knowledge Infrastructure (CNKI), National Digital Science Library (NDSL), Journal of Oriental Neuropsychiatry (JON), Korean Medical Database (KMBASE), KoreaMed, and OASIS, from October 2007 to August 2017, without language restriction. A methodological quality assessment of RCTs or SRs that met inclusion criteria was conducted using Cochrane Risk of bias (RoB) tool and a meta-analysis by RevMan (Review Manager) 5.3.5 version of Cochrane collaboration.

Results: Five RCTs with 257 patients met inclusion criteria and those were randomly divided into two groups: the EA group (n = 103) and Western medications group (n = 154). The methodological quality of the included studies showed high risk or/and unclear of risk of bias. The meta-analysis of five studies reported that the EA group was better than the Western medications group, improving the Mini Mental State Examination (MMSE) score by 0.65 [95% CI 0.28~1.01] higher mean difference, Montreal Cognitive Assessment (MoCA) score by 0.66 [95% CI 0.00~1.32] higher mean difference. Adverse effects were not reported in the selected studies.

Conclusion: Electroacupuncture was an effective treatment for MCI patients by improving cognitive function. However, the included studies presented a low methodological quality and no adverse effects were reported. Thus, further comprehensive studies with a design in depth are needed to derive significant results.
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http://dx.doi.org/10.1186/s12906-018-2407-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325879PMC
January 2019

Stability of pre-orthodontic orthognathic surgery depending on mandibular surgical techniques: SSRO vs IVRO.

J Craniomaxillofac Surg 2016 Sep 25;44(9):1209-15. Epub 2016 Jul 25.

Department of Orthodontics, Institute of Craniofacial Deformity, College of Dentistry, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea. Electronic address:

The aim of this retrospective cohort study was to evaluate the postoperative stability of sagittal split ramus osteotomy (SSRO) and intraoral vertical ramus osteotomy (IVRO) in pre-orthodontic orthognathic surgery (POGS) for skeletal Class III malocclusion. Thirty-seven patients (SSRO, n = 18; IVRO, n = 19) who underwent bimaxillary surgery were divided into two groups according to the type of surgery. During the postoperative period, there were no significant differences in anterior and superior movements of the mandible at point B between the two groups, but occlusal plane angle of the SSRO group significantly decreased more than that of the IVRO group (P = 0.003). Only the SSRO group showed a linear relationship between the amount of postoperative horizontal and vertical movements of the mandible (R(2) = 0.254; P = 0.033), indicating that the amount of postoperative upward movement of the mandible increased as the amount of postoperative forward movement increased (r = -0.504; P = 0.033). The mandible after SSRO in POGS rotated counterclockwise due to rigid fixation between two segments, whereas the mandible after IVRO without rigid fixation in POGS moved mainly in a superior direction. These differences must be considered before surgery to ensure postsurgical stability for patients with mandibular prognathism.
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http://dx.doi.org/10.1016/j.jcms.2016.07.020DOI Listing
September 2016

Acupuncture lowers blood pressure in mild hypertension patients: a randomized, controlled, assessor-blinded pilot trial.

Complement Ther Med 2015 Oct 15;23(5):658-65. Epub 2015 Jul 15.

Korea Institute of Oriental Medicine, Daejeon, South Korea; Korea University of Science and Technology, Daejeon, South Korea. Electronic address:

Objectives: To preliminarily assess the effects of acupuncture on prehypertension and stage I hypertension, and to provide data for further research.

Design: A randomized, controlled, assessor-blinded study with an 8-week intervention period and a 4-week follow-up.

Interventions: Participants were patients with systolic blood pressure (SBP) of 120-159mmHg or diastolic blood pressure (DBP) of 80-99mmHg.Thirty participants were allocated to acupuncture group or untreated control group at a 1:1 ratio. The acupuncture group received standard acupuncture twice weekly for 8 weeks, and was followed-up for 4 weeks after treatment; the control group did not receive any type of anti-hypertensive treatment for 12 weeks.

Main Outcome Measures: Primary outcome measure was SBP and DBP at post-treatment. The secondary outcomes were SBP and DBP at follow-up; Euro Quality of life (EQ-5D), heart rate variability (HRV), body mass index (BMI), and blood lipid profile.

Results: DBP (-5.7mmHg; P=0.025), but not SBP (-6.0mmHg; P=0.123), was significantly different between groups at post-treatment. Both DBP (-7.8mmHg; P=0.004) and SBP (-8.6mmHg; P=0.031) were significantly different at follow-up. Among the HRV indices, only high frequency power was significantly different between groups at weeks 4 and 8 (P=0.047 and P=0.030, respectively). There were no differences between groups in EQ-5D, BMI or lipid profile.

Conclusion: The results of this study show that acupuncture might lower blood pressure in prehypertension and stage I hypertension, and further RCT need 97 participants in each group. The effect of acupuncture on prehypertension and mild hypertension should be confirmed in larger studies.

Trial Registration: KCT0000496.
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http://dx.doi.org/10.1016/j.ctim.2015.06.014DOI Listing
October 2015

Synthesis of (2-amino)ethyl derivatives of quercetin 3-O-methyl ether and their antioxidant and neuroprotective effects.

Bioorg Med Chem 2015 Aug 21;23(15):4970-4979. Epub 2015 May 21.

Medicinal Chemistry Laboratory, Department of Pharmacy, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address:

Reactive oxygen species have been implicated in several diseases, particularly in ischemia-reperfusion injury. Quercetin 3-O-methyl ether has been reported to show potent antioxidant and neuroprotective activity against neuronal damage induced by reactive oxygen species. Several aminoethyl-substituted derivatives of quercetin 3-O-methyl ether have been synthesized to increase water solubility while retaining antioxidant and neuroprotective activity. Among such derivatives, compound 3a shows potent and well-balanced antioxidant activity in three types of cell-free assay systems and has in vivo neuroprotective effects on transient focal ischemic injury induced by the occlusion of the middle cerebral artery in rats.
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http://dx.doi.org/10.1016/j.bmc.2015.05.023DOI Listing
August 2015

Chemoselective reduction and oxidation of ketones in water through control of the electron transfer pathway.

Sci Rep 2015 May 28;5:10366. Epub 2015 May 28.

1] Department of Energy Science, Sungkyunkwan University, Suwon 440-746, Republic of Korea [2] IBS Centre for Integrated Nanostructure Physics, Institute for Basic Science, Suwon, Republic of Korea.

The selective synthesis of different products from the same starting materials in water, which is the most abundant solvent in nature, is a crucial issue as it maximizes the utilization of materials. Realizing such reactions for ketones is of considerable importance because numerous organic functionalities can be obtained via nucleophilic addition reactions. Herein, we report chemoselective reduction and oxidation reactions of 1,2-diketones in water, which initiates anionic electron transfer from the inorganic electride [Ca24Al28O64](4+)·4e(-), through controlling the pathway of the electrons to substrates. The generation of different radical species for transient intermediates was the key process required to control the reaction selectivity, which was achieved by reacting the anionic electrons with either diketones or O2, leading to the formation of ketyl dianion and superoxide radicals in the reduction and oxidation reactions, respectively. This methodology that utilizes electrides may provide an alternative to the pulse radiolysis of water in synthetic chemistry.
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http://dx.doi.org/10.1038/srep10366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603701PMC
May 2015

Correlation between obesity and clinicopathological factors in patients with papillary thyroid cancer.

Surg Today 2015 Jun 25;45(6):723-9. Epub 2014 Jul 25.

Department of Surgery, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul, 137-701, Republic of Korea.

Purpose: Epidemiological studies have shown that obesity is associated with an increased risk of thyroid cancer. However, the exact nature of the relationship, especially with respect to the behavior of the cancer, remains uncertain. The objective of this study was to evaluate the correlation between the body mass index (BMI) and clinicopathological features of thyroid cancer patients.

Methods: From January 2009 to April 2010, 716 consecutive patients (602 females and 114 males; mean age 47.02 ± 11.73 years) with papillary thyroid cancer (PTC) were analyzed retrospectively. Patients were divided into two subgroups according to age (<45 years, ≥45 years). The BMI groupings were based on standardized categories set by the World Health Organization. The relationships between the BMI and these parameters were assessed.

Results: A non-overweight BMI was associated with a younger age and female gender. Tumor multiplicity was related to a higher BMI. In an age-related subgroup analysis, a higher BMI was correlated with more lymph node involvement (p = 0.004), lymphatic invasion (p = 0.003) and tumor multiplicity (p = 0.008) in patients ≥45 years of age. The absence of an association between the BMI and T stage, nodal status, vascular invasion, lymphatic invasion, and extrathyroidal extension was noted in a statistical analysis. In the subgroup of patients <45 years of age, no positive associations were observed between the BMI and any parameters other than age and sex.

Conclusions: In PTC patients ≥45 years of age, a higher BMI was associated with more aggressive tumor features, such as lymph node metastasis, lymphatic invasion, and tumor multiplicity.
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http://dx.doi.org/10.1007/s00595-014-0984-3DOI Listing
June 2015

A novel quartz crystal microbalance gas sensor based on porous film coatings. A high sensitivity porous poly(methylmethacrylate) water vapor sensor.

Anal Chim Acta 2013 Jun 6;785:98-103. Epub 2013 May 6.

Department of Chemistry, University at Buffalo, SUNY, Buffalo, NY 14260-3000, USA.

We describe a novel and generally applicable approach for creating voids in films deposited on the surface of solid substrates. Such films are advantageous when a quartz crystal microbalance (QCM) is the basis of a sensor. We show that films with large void volumes produce more sensitive sensors than with the original film. Poly(methylmethacrylate) (PMMA) was used as the polymer layer deposited on a quartz crystal microbalance (QCM) to demonstrate our technique for the model system of water vapor analysis in flowing nitrogen gas. A film of pure PMMA on a QCM is a sensor for water vapor in a gas phase. A more sensitive sensor was created by dip coating QCM crystals into solutions containing mixtures of PMMA and poly(D,L-lactide) (PDLL) and then evaporating the solution films on the QCM crystals to form mixed polymer films of varying PDLL content. The PDLL was then removed from the mixed polymer films by exposure to a NaOH solution to form pure PMMA films having various void volumes. A leached PMMA film that originally contained 50% by weight PDLL had a 3.7 times larger QCM sensitivity for water vapor than a pure PMMA film.
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http://dx.doi.org/10.1016/j.aca.2013.04.052DOI Listing
June 2013

Comparison of Metabolic Characteristics of Metabolically Healthy but Obese (MHO) Middle-Aged Men According to Different Criteria.

Korean J Fam Med 2013 Jan 28;34(1):19-26. Epub 2013 Jan 28.

Department of Family Medicine, Dankook University College of Medicine, Cheonan, Korea.

Background: To compare the prevalence and metabolic characteristics of metabolically healthy but obese (MHO) individuals according to different criteria.

Methods: We examined 186 MHO middle-aged men (age, 37.2 years; body mass index [BMI], 27.2 kg/m(2)). The following methods were used to determine MHO: the National Cholesterol Education Program (NCEP) Adult Treatment Panel III criteria, 0-2 cardiometabolic abnormalities; the Wildman criteria, 0-1 cardiometabolic abnormalities; the Karelis criteria, 0-1 cardiometabolic abnormalities; the homeostasis model assessment [HOMA] criteria (lowest quartile of HOMA). After dividing the overall subjects into two age groups, we compared the prevalence and clinical characteristics between MHO and at-risk groups according to four different criteria.

Results: The prevalence of MHO using the NCEP, Wildman, Kaleris, and HOMA criteria were 70.4%, 59.7%, 28.5%, and 24.2%, respectively. The agreement between the groups according to the NCEP and Wildman criteria was substantial (kappa = 0.8, P < 0.001). Among individuals 35 years or younger, and regardless of method, the MHO subjects had significantly lower weight, waist circumference, BMI, body fat percentage, insulin, HOMA, alanine aminotransferase, triglyceride (TG), and TG/high density lipoprotein cholesterol (HDL-C) ratio than the at-risk subjects (P < 0.05); However, among individuals older than 35 years old, and regardless of method, the MHO subjects had different insulin, HOMA, HDL-C, and TG/HDL-C levels than the at-risk subjects (P < 0.05).

Conclusion: The differences in metabolic profile between MHO and at-risk groups varied according to age. MHO prevalence varies considerably according to the criteria employed. Expert consensus is needed in order to define a standardized protocol for determining MHO.
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http://dx.doi.org/10.4082/kjfm.2013.34.1.19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560335PMC
January 2013

Pattern of distant recurrence according to the molecular subtypes in Korean women with breast cancer.

World J Surg Oncol 2012 Jan 8;10. Epub 2012 Jan 8.

Department of Surgery, The Catholic University of Korea College of Medicine, Seoul, Korea.

Background: Distant recurrence is one of the most important risk factors in overall survival, and distant recurrence is related to a complex biologic interaction of seed and soil factors. The aim of the study was to investigate the association between the molecular subtypes and patterns of distant recurrence in patients with breast cancer.

Methods: In an investigation of 313 women with breast cancer who underwent surgery from 1994 and 2000, the expressions of estrogen and progestrone receptor (ER/PR), and human epithelial receptor-2 (HER2) were evaluated. The subtypes were defined as luminal-A, luminal-HER2, HER2-enriched, and triple negative breast cancer (TNBC) according to ER, PR, and HER2 status.

Results: Bone was the most common site of distant recurrence. The incidence of first distant recurrence site was significantly different among the subtypes. Brain metastasis was more frequent in the luminal-HER2 and TNBC subtypes. In subgroup analysis, overall survival in patients with distant recurrence after 24 months after surgery was significantly different among the subtypes.

Conclusions: Organ-specific metastasis may depend on the molecular subtype of breast cancer. Tailored strategies against distant metastasis concerning the molecular subtypes in breast cancer may be considered.
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http://dx.doi.org/10.1186/1477-7819-10-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286379PMC
January 2012

Comparison of surgical outcomes between endoscopic and robotic thyroidectomy.

J Surg Oncol 2012 Jun 27;105(7):705-8. Epub 2011 Sep 27.

Department of Surgery, The Catholic University of Korea, Korea.

Objectives: Gasless transaxillary endoscopic thyroidectomy offers a distinct advantage over the conventional open operation because it leaves no visible neck scar. Indications have expanded with the development of new surgical techniques and instruments. However, because of the two-dimensional view and the nonflexible instruments, this procedure is not easily amenable for total thyroidectomy. So, robotic surgery has been introduced and offers improved visualization and dexterity. But, it remains unclear whether robotic thyroidectomy offers any potential benefits over endoscopic thyroidectomy. The aim of this present study was to determine whether robotic surgery is superior to endoscopic surgery through a comparison of surgical outcomes.

Methods: Between May 2009 and February 2011, 165 patients underwent endoscopic thyroidectomy (endoscopy group) and 46 patients underwent robotic thyroidectomy (robot group). A gasless transaxillary approach was used in both groups. The two groups were compared in terms of patient characteristics, perioperative clinical results, pathologic findings, and postoperative complication.

Results: Both patient groups were similar in terms of patient characteristics, mean number of retrieved central lymph nodes, pathological features, length of hospital stays, postoperative complication rate, and serum Tg level. However, the mean total operation time for thyroidectomy was 126.2 ± 37.84 min in the endoscopy group and 179.6 ± 44.34 min in the robot group (P < 0.001). Postoperative total drainage for lobectomy was 153.3 ± 45.64 for the endoscopy group and 179.9 ± 49.15 for the robot group (P = 0.031). Cost effectiveness is also an important consideration when evidence for predominance of two surgical techniques is lacking. The mean cost of robotic thyroidectomy was $6,655, compared with $829 for endoscopic thyroidectomy (P < 0.001). There was no significant difference in postoperative complications as hypocalcemia, recurrent laryngeal nerve injury, chyle leakage and tracheal injury in the two groups (P = 0.332).

Conclusion: Robotic thyroidectomy was lengthier in duration than endoscopic thyroidectomy, more costly, and associated with increased postoperative drainage with no improvement in oncologic outcomes or complication rates. Therefore our data do not support any advantage of robotic surgery over endoscopic surgery.
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http://dx.doi.org/10.1002/jso.22106DOI Listing
June 2012

Impact of developmental maturity of soybean on the seasonal abundance of soybean aphid (Hemiptera: Aphididae).

Environ Entomol 2010 Apr;39(2):484-91

Department of Entomology, Purdue University, Smith Hall, 901 West State St, West Lafayette, IN 47907-2089, USA.

The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), alternates between a primary overwintering host (buckthorn, Rhamnus sp.) and a secondary summer host (soybean, Glycine max). Selection of soybean cultivars with different maturity groups may provide growers with a management tool for A. glycines, either directly through its effect on summer populations that cause economic damage or indirectly through its effect on the production of migrants that disperse to the primary host in fall. This study investigated the abundance and seasonality of A. glycines on soybean cultivars with different maturity rates in central Indiana. The abscission of soybean foliage occurred earlier for early maturing than late maturing cultivars, but no other consistent difference in development or yield was detected among the cultivars tested in this study. The abundance of aphids did not vary consistently among cultivars when soybean was most susceptible to economic damage. A laboratory assay evaluating the larviposition preference of A. glycines alate females, combined with a 7-yr survey documenting the colonization of buckthorn by winged aphids, indicated that the production of gynoparae on soybean began in mid-September and continued until leaf abscission. The abundance of aphids during this period was higher on late maturing cultivars than on early maturing cultivars in both 2006 and 2008, whereas no significant effect was detected in 2007. Altogether, these results suggest that planting early maturing soybean cultivars has little effect on damage by aphids on the current season crop but may reduce the number of fall migrants to the primary host.
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http://dx.doi.org/10.1603/EN09034DOI Listing
April 2010

Potential of suction traps as a monitoring tool for Aphis glycines (Hemiptera: Aphididae) in soybean fields.

J Econ Entomol 2010 Feb;103(1):186-9

Department of Entomology, Purdue University, Smith Hall, 901 West State Street, West Lafayette, IN 47907, USA.

The current study evaluated the potential of using counts of winged adults captured in suction traps to forecast the local abundance of soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), in soybean, Glycine max (L.) Merr., fields. The abundance of aphids was evaluated weekly by sampling plants in four to 11 soybean fields and recording the number of aphids in suction traps between 2006 and 2008 in four counties in Indiana and Illinois. Fields in each county were located within 10 km of their respective suction trap, which allowed us to evaluate the relation between aphid abundance on soybean plants and in suction traps at the county level. Migrant soybean aphids caught in suction traps exhibited distinct seasonal trends each year: in 2006, trapped migrants consisted predominantly of individuals dispersing from soybean to buckthorn (Rhamnus sp.); in 2007, in contrast, the majority of trapped migrants were apparently individuals dispersing among soybean fields. The cumulative number of aphids captured in suction traps was positively related to aphid densities on soybean plants. However, the utility of suction traps as a monitoring tool may be limited by the variation in temporal patterns observed in suction traps and on soybean plants each year, and the spatial variation in aphid abundance among soybean fields within a county.
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http://dx.doi.org/10.1603/ec09121DOI Listing
February 2010

Tracking the role of alternative prey in soybean aphid predation by Orius insidiosus: a molecular approach.

Mol Ecol 2007 Oct 4;16(20):4390-400. Epub 2007 Sep 4.

Department of Entomology, University of Kentucky, S-225 Agricultural Science Center North, Lexington, Kentucky 40546-0091, USA.

The soybean aphid, Aphis glycines (Hemiptera: Aphididae), is a pest of soybeans in Asia, and in recent years has caused extensive damage to soybeans in North America. Within these agroecosystems, generalist predators form an important component of the assemblage of natural enemies, and can exert significant pressure on prey populations. These food webs are complex and molecular gut-content analyses offer nondisruptive approaches for examining trophic linkages in the field. We describe the development of a molecular detection system to examine the feeding behaviour of Orius insidiosus (Hemiptera: Anthocoridae) upon soybean aphids, an alternative prey item, Neohydatothrips variabilis (Thysanoptera: Thripidae), and an intraguild prey species, Harmonia axyridis (Coleoptera: Coccinellidae). Specific primer pairs were designed to target prey and were used to examine key trophic connections within this soybean food web. In total, 32% of O. insidiosus were found to have preyed upon A. glycines, but disproportionately high consumption occurred early in the season, when aphid densities were low. The intensity of early season predation indicates that O. insidiosus are important biological control agents of A. glycines, although data suggest that N. variabilis constitute a significant proportion of the diet of these generalist predators. No Orius were found to contain DNA of H. axyridis, suggesting intraguild predation upon these important late-season predators during 2005 was low. In their entirety, these results implicate O. insidiosus as a valuable natural enemy of A. glycines in this soybean agroecosystem.
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http://dx.doi.org/10.1111/j.1365-294X.2007.03482.xDOI Listing
October 2007

Voluntary exercise and clomipramine treatment elevate prepro-galanin mRNA levels in the locus coeruleus in rats.

Neurosci Lett 2006 Nov 22;408(1):1-4. Epub 2006 Sep 22.

Neuroscience and Behavior Program, Psychology Department, The University of Georgia, Athens, GA 30602, USA.

Exercise exerts antidepressant effects in humans and rodent models of affective disorders. These effects may be mediated by the upregulation of endogenous factors that exert antidepressant actions. The physiological functions and behavioral actions of the neuropeptide galanin (GAL) suggest antidepressant activity. Previous studies have shown that various modes of exercise elevate GAL gene expression in the locus coeruleus (LC) in rats. The present experiments examined the interaction between voluntary exercise and antidepressant pharmacotherapy. Male Sprague-Dawley rats were provided access to activity wheels (exercise condition) or inoperative wheels (sedentary condition) for 28 days. Rats in each group were injected with clomipramine (10mg/kg/day) or vehicle throughout this period (for 3 weeks). Prepro-GAL mRNA in the LC was measured by in situ hybridization histochemistry. Exercise and clomipramine treatment significantly elevated GAL gene expression, though prepro-GAL mRNA levels in rats receiving both interventions did not differ from sedentary controls that received vehicle. Prepro-GAL mRNA levels were significantly correlated with running distance. The results further implicate a role for GAL in the antidepressant effects of exercise and pharmacotherapy, though the mechanisms through which these treatments influence GAL gene expression appear to differ significantly.
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http://dx.doi.org/10.1016/j.neulet.2006.04.057DOI Listing
November 2006

Local population size in a flightless insect: importance of patch structure-dependent mortality.

Authors:
Ho Jung S Yoo

Ecology 2006 Mar;87(3):634-47

Department of Ecology, Evolution, and Marine Biology, University of California, Santa Barbara, California 93106-9610, USA.

In spatially heterogeneous systems, utilizing population models to integrate the effects of multiple population rates can yield powerful insights into the relative importance of the component rates. The relative importance of demographic rates and dispersal in shaping the distribution of the western tussock moth (Orgyia vetusta) among patches of its host plant was explored using stage-structured population models. Tussock moth dispersal occurs passively in first-instar larvae and is poor or absent in all other life stages. Spatial surveys suggested, however, that moth distribution is not well explained by passive dispersal; moth populations were greater on small patches and on isolated ones. Further analysis showed that several local demographic rates varied significantly with patch characteristics. Two mortality factors in particular may explain the observed patterns. First, crawler mortality both increased with patch size and was density-dependent. A single-patch difference equation model showed mortality related to patch size is strong enough to overcome the homogenizing effect of density dependence; greater equilibrium densities were predicted for smaller patches. Second, although three rates were found to vary with local patch density, only pupal parasitism by a chalcid wasp could potentially account for higher moth abundances on isolated patches. A spatially explicit simulation model of the multiple-patch system showed that spatial variation in pupal parasitism is indeed strong enough to generate such a pattern. These results demonstrate that habitat spatial structure can affect multiple population processes simultaneously, and even relatively low attack rates imposed on a reproductively valuable life stage of the host can have a dominant effect on population distribution among habitat patches.
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http://dx.doi.org/10.1890/05-0509DOI Listing
March 2006

Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase.

FEBS Lett 2005 Feb 24;579(5):1243-8. Epub 2005 Jan 24.

Division of Molecular Life Science, Center for Cell Signaling Research, Ewha Womans University, Seoul 120-750, Korea.

We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism.
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http://dx.doi.org/10.1016/j.febslet.2005.01.018DOI Listing
February 2005

TIMP-1 inhibits apoptosis in breast carcinoma cells via a pathway involving pertussis toxin-sensitive G protein and c-Src.

Biochem Biophys Res Commun 2003 Dec;312(4):1196-201

Department of Biochemistry, College of Science, and Protein Network Research Center, Yonsei University, Seoul, Republic of Korea.

In addition to inhibiting matrix metalloproteinases, tissue inhibitor of metalloproteinase-1 (TIMP-1) is involved in the regulation of cell growth and survival. To determine its mechanism of action, we investigated effects of TIMP-1 on cell proliferation and survival and signaling pathways induced by TIMP-1 in the human breast carcinoma T-47D cell line. Treatment of T-47D cells with TIMP-1 strongly inhibited apoptosis induced by serum deprivation, but did not affect cell proliferation. TIMP-1 induced phosphorylation of Akt and extracellular signal-regulated protein kinases (ERKs), but pertussis toxin and specific inhibitors of Src family tyrosine kinases, protein tyrosine kinases, and phosphatidylinositol-3 kinase (PI3 kinase) blocked the ability of TIMP-1 to activate Akt and ERKs as well as the anti-apoptotic effect of TIMP-1. We found that TIMP-1 enhanced the kinase activities of c-Src and PI3 kinase and that this enhancement was inhibited by pertussis toxin. Inhibition of ERK activation, however, resulted in a slight decrease of the TIMP-1-induced anti-apoptotic effect. These findings demonstrate that the ability of TIMP-1 to inhibit apoptosis in T-47D cells is mediated by the sequential activation of pertussis toxin-sensitive G protein, c-Src, PI3 kinase, and Akt.
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http://dx.doi.org/10.1016/j.bbrc.2003.11.050DOI Listing
December 2003

Synthesis of styrylbenzofuran derivatives as styrylquinoline analogues for HIV-1 integrase inhibitor.

Farmaco 2003 Dec;58(12):1243-50

Medicinal Chemistry Research Center, Life Sciences Division, Korea Institute of Science & Technology, P.O. Box 131 Cheongryang, Seoul 130-650, South Korea.

A series of styrylbenzofuran derivatives (8a-i) as styrylquinoline isosters were efficiently prepared by Wittig reaction and evaluated for inhibitory activity against HIV-1 integrase. In this series, compounds 8g, 8h and 8i containing a free catechol ring showed moderate inhibitory activities (IC50= approximately 40 microM) against HIV-1 integrase, while less than the corresponding styrylquinoline compound (I).
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http://dx.doi.org/10.1016/j.farmac.2003.08.001DOI Listing
December 2003
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