Publications


Molecular mechanism underlying promiscuous polyamine recognition by spermidine acetyltransferase.
Int J Biochem Cell Biol 2016 Jul 6;76:87-97. Epub 2016 May 6.
SOSHO Inc., Suita, Osaka 565-0871, Japan; Department of Biotechnology, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. Electronic address:



Structure-based design of a streptavidin mutant specific for an artificial biotin analogue.
J Biochem 2015 Jun 2;157(6):467-75. Epub 2015 Feb 2.
Division of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 153-8904, Japan; and Radioisotope Center, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan


The N-terminal acidic residue of the cytosolic helix 8 of an odorant receptor is responsible for different response dynamics via G-protein.
FEBS Lett 2015 Apr 31;589(10):1136-42. Epub 2015 Mar 31.
Health Research Institute, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan. Electronic address:

Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of a seed storage protein.
Acta Crystallogr F Struct Biol Commun 2015 Feb 28;71(Pt 2):132-5. Epub 2015 Jan 28.
Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.


Unliganded estrogen receptor α stimulates bone sialoprotein gene expression.
Gene 2014 Apr 2;539(1):50-7. Epub 2014 Feb 2.
Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan; Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan. Electronic address:

Strategy for cold adaptation of the tryptophan synthase α subunit from the psychrophile Shewanella frigidimarina K14-2: crystal structure and physicochemical properties.
J Biochem 2014 Feb 25;155(2):73-82. Epub 2013 Oct 25.
Department of Ocean Sciences, Graduate school of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato, Tokyo 108-8477; Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871; Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871; Department of Biomolecular Chemistry, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522; Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871; Department of Life Science, University of Hyogo, 3-2-1, Kouto, Kamigori, Ako-gun, Hyogo 678-1297; and RIKEN SPring-8 Center, RIKEN Harima Institute, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148.

Structural insights into the function of a thermostable copper-containing nitrite reductase.
J Biochem 2014 Feb 30;155(2):123-35. Epub 2013 Nov 30.
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; Department of Applied Chemistry, School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Faculty of Life and Environmental Sciences, Department of Environmental Information, Kyoto Prefectural University, Shimogamo-Hanki-cho, Sakyou, Kyoto 606-8522, Japan; Molecular-Recognition Structure Analysis Team, Molecular Profiling Research Center for Drug Discovery (molprof) National Institute of Advanced Industrial Science and Technology (AIST) 2-3-26 Aomi, Koto-ku, Tokyo 135-0064, Japan; Microbial Genome Research Group, Japan Agency of Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan; and RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.


Expression, purification, crystallization and preliminary crystallographic study of FtsA from methicillin-resistant Staphylococcus aureus.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2013 Aug 27;69(Pt 8):895-8. Epub 2013 Jul 27.
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Crystal structure of rice Rubisco and implications for activation induced by positive effectors NADPH and 6-phosphogluconate.
J Mol Biol 2012 Sep 17;422(1):75-86. Epub 2012 May 17.
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan.


Crystallization and preliminary X-ray diffraction analysis of orotate phosphoribosyltransferase from the human malaria parasite Plasmodium falciparum.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2012 Feb 27;68(Pt 2):244-6. Epub 2012 Jan 27.
Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.

Crystallization and preliminary X-ray crystallographic analysis of a helicase-like domain from a tomato mosaic virus replication protein.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2011 Dec 30;67(Pt 12):1649-52. Epub 2011 Nov 30.
Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

Structure basis for the regulation of glyceraldehyde-3-phosphate dehydrogenase activity via the intrinsically disordered protein CP12.
Structure 2011 Dec;19(12):1846-54
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

cAMP and fibroblast growth factor 2 regulate bone sialoprotein gene expression in human prostate cancer cells.
Gene 2011 Jan 19;471(1-2):1-12. Epub 2010 Oct 19.
Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan; Tianjin Stomatology Hospital, Tianjin, China.

Conformational plasticity of RNA for target recognition as revealed by the 2.15 A crystal structure of a human IgG-aptamer complex.
Nucleic Acids Res 2010 Nov 30;38(21):7822-9. Epub 2010 Jul 30.
Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, Narashino-shi, Chiba 275-0016, Japan.

Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase.
J Biol Chem 2009 Apr 16;284(14):9566-77. Epub 2008 Dec 16.
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-Oka, Suita, Osaka 565-0871, Japan.

Structure of HIV-1 protease in complex with potent inhibitor KNI-272 determined by high-resolution X-ray and neutron crystallography.
Proc Natl Acad Sci U S A 2009 Mar 9;106(12):4641-6. Epub 2009 Mar 9.
Molecular Structural Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan.

Crystallization and preliminary X-ray analysis of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2009 Feb 31;65(Pt 2):147-50. Epub 2009 Jan 31.
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Crystallization and preliminary X-ray crystallographic analysis of Ca2+-free primary Ca2+-sensor of Na+/Ca2+ exchanger.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2008 Dec 28;64(Pt 12):1125-7. Epub 2008 Nov 28.
Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka 569-1094, Japan.




Requirement of left-handed glycine residue for high stability of the Tk-subtilisin propeptide as revealed by mutational and crystallographic analyses.
J Mol Biol 2007 Dec 17;374(5):1359-73. Epub 2007 Oct 17.
Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Crystallization and preliminary X-ray diffraction study of glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2007 Feb 27;63(Pt 2):126-9. Epub 2007 Jan 27.
Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Application of femtosecond laser ablation for detaching grown protein crystals from glass capillary tube.
J Biosci Bioeng 2006 Oct;102(4):372-4
Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

Crystallization and preliminary X-ray diffraction study of an active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 Sep 18;62(Pt 9):902-5. Epub 2006 Aug 18.
Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Crystallization and preliminary crystallographic analysis of orotidine 5'-monophosphate decarboxylase from the human malaria parasite Plasmodium falciparum.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 Jun 31;62(Pt 6):542-5. Epub 2006 May 31.
Unit of Biochemistry, Department of Medical Science, Faculty of Science, Rangsit University, Patumthani 12000, Thailand.


Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA(Glu).
Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 Apr 10;62(Pt 4):368-71. Epub 2006 Mar 10.
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8501, Japan.


Overproduction and preliminary crystallographic study of a human kynurenine aminotransferase II homologue from Pyrococcus horikoshii OT3.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2005 Mar 24;61(Pt 3):319-22. Epub 2005 Feb 24.
Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Crystallization and preliminary X-ray diffraction analysis of thioredoxin peroxidase from the aerobic hyperthermophilic archaeon Aeropyrum pernix K1.
Acta Crystallogr Sect F Struct Biol Cryst Commun 2005 Mar 24;61(Pt 3):323-5. Epub 2005 Feb 24.
National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka 563-8577, Japan.

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